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Understanding PCR

Understanding PCR Book
Author : Sarah Maddocks,Rowena Jenkins
Publisher : Academic Press
Release : 2016-10-27
ISBN : 0128026979
Language : En, Es, Fr & De

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Book Description :

Understanding PCR: A Practical Bench-Top Guide gives you all of the information you need to plan your first PCR, from reagents to conditions to analysis and beyond. It is a user friendly book that has step-by-step basic protocols, which can be adapted to your needs. Includes helpful information such as where to order your reagents and basic troubleshooting hints and tips. Includes resources for reagents Explains basic laboratory preparation Provides straightforward experimental protocols Incorporates fundamental analytical techniques Contains a troubleshooting guide

Principles and Technical Aspects of PCR Amplification

Principles and Technical Aspects of PCR Amplification Book
Author : Elizabeth van Pelt-Verkuil,Alex van Belkum,John P. Hays
Publisher : Springer Science & Business Media
Release : 2008-03-14
ISBN : 9781402062414
Language : En, Es, Fr & De

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Book Description :

Kary Mullis was awarded a Nobel Prize for inventing the PCR technique more than a decade ago in 1993. Since its "discovery", multiple adaptations and variations of the standard PCR technique have been described. This publication aims to provide the reader with a guide to the standard PCR technique and its many available variants, with particular emphasis being placed on the role of these PCR techniques in the clinical diagnostic laboratory (the central theme of this book).

Understanding PCR

Understanding PCR Book
Author : Sarah Maddocks,Rowena Jenkins
Publisher : Academic Press
Release : 2016-11-11
ISBN : 9780128026830
Language : En, Es, Fr & De

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Book Description :

Understanding PCR: A Practical Bench-Top Guide gives you all of the information you need to plan your first PCR, from reagents to conditions to analysis and beyond. It is a user friendly book that has step-by-step basic protocols, which can be adapted to your needs. Includes helpful information such as where to order your reagents and basic troubleshooting hints and tips. Includes resources for reagents Explains basic laboratory preparation Provides straightforward experimental protocols Incorporates fundamental analytical techniques Contains a troubleshooting guide

Understanding Cycle Threshold Ct in SARS CoV 2 RT PCR

Understanding Cycle Threshold  Ct  in SARS CoV 2 RT PCR Book
Author : Anonim
Publisher : Unknown
Release : 2020
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Understanding Cycle Threshold Ct in SARS CoV 2 RT PCR book written by , available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

PCR Protocols in Molecular Toxicology

PCR Protocols in Molecular Toxicology Book
Author : John P. Vanden Heuvel
Publisher : CRC Press
Release : 2019-05-07
ISBN : 1439805806
Language : En, Es, Fr & De

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Book Description :

Molecular toxicology is an emerging discipline that utilizes molecular and cell biology to understand how drugs and chemicals result in their unwanted effects. PCR Protocols in Molecular Toxicology is a practical guide to the use of polymerase chain reaction (PCR) to help examine, on a molecular and cellular level, how toxic responses are manifested. It offers a basic understanding of PCR and its optimization, as well as describing specific, high-impact areas of molecular toxicology and recent advances. The following techniques are described in detail: Quantitative reverse transcriptase PCR and methods to examine gene expression Differential display cloning Cloning and library screening by PCR Genotype and polymorphism analysis of drug and toxicant metabolizing enzymes Basic, non-PCR based molecular biology methods PCR Protocols in Molecular Toxicology will aid both novices and experienced PCR practitioners in using PCR to its fullest potential.

Applications of PCR in Mycology

Applications of PCR in Mycology Book
Author : P. D. Bridge
Publisher : CABI
Release : 1998-01-01
ISBN : 0851992331
Language : En, Es, Fr & De

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Book Description :

Since the initial report of the amplification of specific DNA fragments using the polymerase chain reaction (PCR) in 1985, this technique has revolutionized molecular biology. It enables the production of large quantities of DNA from minute amounts of sample material, which can then be readily analyzed. This facility has had an enormous influence on the way both fundamental and diagnostic questions are approached and its use is now considered essential for molecular work in all branches of biology. The purpose of this book is to highlight the wide-ranging applications of PCR in pure and applied mycology and to increase understanding of its potential benefits. After a brief overview, a group of internationally-renowned mycologists give definitive descriptions of the use of PCR in their own specialized fields. These include fungal gene expression and cloning, taxonomy and speciation, fungal mycobionts, mycorrhizal fungi, entomopathogenic fungi, mycotoxin-producing fungi, diagnosis of fungal infections in animals, seed-borne diseases, fungal/plant interactions and applications with industrially-important fungi. Finally, potential future directions for PCR work in mycology are discussed.

PCR

PCR Book
Author : Mike McPherson,Simon Møller
Publisher : Garland Science
Release : 2007-01-24
ISBN : 1134254555
Language : En, Es, Fr & De

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Book Description :

A thoroughly updated version of the successful first edition with a new chapter on Real-Time PCR, more prokaryotic applications, and more detail in the complex mutagenesis sections. Information on PCR applications in genomics and proteomics have been expanded and integrated throughout the text. There is also advice on available products and specific pointers to the most appropriate methods. As with the first edition, this will be an ideal practical introduction and invaluable guide to PCR and its applications.

Principles and Technical Aspects of PCR Amplification

Principles and Technical Aspects of PCR Amplification Book
Author : Elizabeth van Pelt-Verkuil,Alex van Belkum,John P. Hays
Publisher : Springer
Release : 2008-03-28
ISBN : 9781402062407
Language : En, Es, Fr & De

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Book Description :

Kary Mullis was awarded a Nobel Prize for inventing the PCR technique more than a decade ago in 1993. Since its "discovery", multiple adaptations and variations of the standard PCR technique have been described. This publication aims to provide the reader with a guide to the standard PCR technique and its many available variants, with particular emphasis being placed on the role of these PCR techniques in the clinical diagnostic laboratory (the central theme of this book).

Stable Isotopes to Trace Migratory Birds and to Identify Harmful Diseases

Stable Isotopes to Trace Migratory Birds and to Identify Harmful Diseases Book
Author : G.J. Viljoen,A.G. Luckins,I. Naletoski
Publisher : Springer
Release : 2016-06-22
ISBN : 3319282980
Language : En, Es, Fr & De

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Book Description :

This manuscript discusses the potentials of the approaches as mentioned below to monitor the AIVs in WMW. Molecular diagnostic platforms enable for accurate detection of the AIVs in the feces of infected birds. Similar technologies can be used to determine the bird species through DNA barcoding, enabling non-invasive research on the epidemiology of the disease. Wild migratory waterfowl (WMW) play significant role in the transmission of avian influenza viruses (AIVs) on large distances. Understanding bird migrations may therefore significantly contribute towards understanding of the disease epidemiology, however most conventional approaches to trace WMW migrations are based on capturing, tagging (mostly ringing or GPS devices) and their re-capturing to link the departure and arrival places. Stable isotope ratios in metabolically inert tissues (feathers, beaks, claws) reflect the ratios present at the point of intake (drinking or feeding), thus enabling for tracing bird origins at stopover places. Molecular diagnostic platforms such as the polymerase chain reaction (PCR) enable for accurate detection of the AIVs in the feces of infected birds. Similar technologies (genetic sequencing) can be used to determine the bird species through DNA barcoding. Simple and easy collection of feather and fecal samples at the stopover places may generate a full information package on which species of WMW carries the AIVs (PCR+DNA barcoding on the feces), as well as the origin of these species (SI+DNA barcoding on the feathers). Therefore, such approaches enable for research on the epidemiology and the ecology of the AIVs in WMW using a non-invasive platform, which does not require capturing of WMW. This manuscript discusses the potentials of these approaches to monitor the AIVs in WMW. p>

PCR Strategies

PCR Strategies Book
Author : Michael A. Innis,David H. Gelfand,John J. Sninsky
Publisher : Elsevier
Release : 1995-07-06
ISBN : 0080538541
Language : En, Es, Fr & De

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Book Description :

PCR Strategies expands and updates the landmark volume PCR Protocols. It is a companion laboratory manual that provides a completely new set of up-to-date strategies and protocols for getting the most from PCR. The editors have organized the book into four sections, focusing on principles, analyses, research applications, and alternative strategies for a wide variety of basic and clinical needs. If you own PCR Protocols, you will want PCR Strategies. If you don't own PCR Protocols, you will want to buy both! Concepts explained Methods detailed Trouble-shooting emphasized Novel applications highlighted Key concepts for PCR Analysis of PCR products Research applications Alternative amplification strategies

Understanding Sporulation and Dissemination of Podosphaera Macularis Hop Powdery Mildew

Understanding Sporulation and Dissemination of Podosphaera Macularis  Hop Powdery Mildew Book
Author : Amy B. Peetz
Publisher : Unknown
Release : 2008
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Podosphaera macularis causes one of the most important diseases, powdery mildew, of Humulus lupulus (hop). If left unmanaged, hop powdery mildew can cause total crop loss due to disease or browning of hop cones rendering the cones unmarketable to buyers. The Hop Powdery Mildew Infection Risk Index (HOPS) is heavily relied on for management of the disease. However, HOPS assumes constant inoculum presence, which is not likely to be true. Evidence suggests that temperature fluctuations influence inoculum availability. As such, knowledge of inoculum presence and density and their relationship to environmental conditions may increase management efficacy by reducing pesticide use and cost of crop production. Molecular methods for detecting and quantifying P. macularis were developed and used to analyze air samples taken from hop fields for two seasons. A protocol for DNA extraction from field samples was modified in order to use PCR to detect and quantify P. macularis DNA from the field. Primers and a probe designed to the internal transcribed spacer (ITS) region of P. macularis ribosomal DNA have made inoculum detection possible using both conventional polymerase chain reaction (PCR) and real-time quantitative PCR (qPCR). DNA extracted from conidia impinged on glass rods coated in silicon grease has been used as template for qPCR, and we have successfully detected as DNA extracted from 10 spores Specific and sensitive detection of P. macularis DNA with conventional PCR was possible under conditions typically used to evaluate qPCR; however results from field samples indicated that sensitivity was an issue, in that spores were not detected in samples from fields with high disease pressure. The effects of constant exposure (5, 10, 15, 20, 25, 30, and 35°C) and 6 hr exposure (18, 22, 26, 30, 34, and 38°C) on sporulation of P. macularis were tested. Exposure to constant low and high temperatures decreases sporulation, which indicates that inoculum may not always be available once the epidemic has started. Sporulation is also decreased during brief exposures to temperatures above 30°C. These data indicate that inoculum availability is reduced when the temperature exceeds 30°C in the field. A modification of HOPS should be examined to incorporate an algorithm which takes the findings in this study into account.

Understanding Viruses

Understanding Viruses Book
Author : Teri Shors
Publisher : Jones & Bartlett Publishers
Release : 2011-11-08
ISBN : 1449677541
Language : En, Es, Fr & De

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Book Description :

The ideal text for undergraduate students majoring in biology, microbiology, medical technology, or pre-med, the Second Edition of Understanding Viruses provides a balanced approach to this fascinating discipline, combining the molecular, clinical, and historical aspects of virology. Updated throughout to keep pace with this fast-paced field, the text provides a strong, comprehensive introduction to human viral diseases. New material on molecular virology as well as new virus families presented coupled with chapters on viral diseases of animals; the history of clinical trials, gene therapy, and xenotranplantation; prions and viroids; plant viruses; and bacteriophages add to the scope of the text. Chapters discussing specific viral diseases weave in an epidemiological and global perspective and include treatment and prevention information. Contemporary case studies, Refresher Boxes, and Virus Files engage students in the learning process. With a wealth of student and instructor support tools, Understanding Viruses is an accessible, exciting, and engaging text for your virology course.

Omics Approaches to Understanding Muscle Biology

Omics Approaches to Understanding Muscle Biology Book
Author : Jatin George Burniston,Yi-Wen Chen
Publisher : Springer Nature
Release : 2019-11-05
ISBN : 1493998021
Language : En, Es, Fr & De

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Book Description :

This book is a collection of principles and current practices in omics research, applied to skeletal muscle physiology and disorders. The various sections are categorized according to the level of biological organization, namely, genomics (DNA), transcriptomics (RNA), proteomics (protein), and metabolomics (metabolite). With skeletal muscle as the unifying theme, and featuring contributions from leading experts in this traditional field of research, it highlights the importance of skeletal muscle tissue in human development, health and successful ageing. It also discusses other fascinating topics like developmental biology, muscular dystrophies, exercise, insulin resistance and atrophy due to disuse, ageing or other muscle diseases, conveying the vast opportunities for generating new hypotheses as well as testing existing hypotheses by combining high-throughput techniques with proper experiment designs, bioinformatics and statistical analyses. Presenting the latest research techniques, this book is a valuable resource for the physiology community, particularly researchers and grad students who want to explore the new opportunities for omics technologies in basic physiology research.

Understanding Cerebral Palsy

Understanding Cerebral Palsy Book
Author : Daniel A. Hatton,Erie County Crippled Children's Society
Publisher : Unknown
Release : 1962
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Understanding Cerebral Palsy book written by Daniel A. Hatton,Erie County Crippled Children's Society, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Conflict and Disaster

Conflict and Disaster Book
Author : Sarah Najmilah
Publisher : Unknown
Release : 2019
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

In recent times, the global community has been dealing with more complex crises than ever before. The pairing of conflict and disaster is one such complex combination that challenges the ability of nations to ensure safety and well-being for their constituents. The growing number of disciplines that have concerned themselves with this area of scholarship indicates a need for an integrated framework in dealing with both issues as they should not be treated separately. This study intends to contribute to the discussion around the conflict and disaster nexus, particularly on how conflict resolution influences the degree of disaster risk. It employs a case study of Aceh, Indonesia, which is considered successful in ending and recovering from a prolonged conflict between Government of Indonesia (GoI) and Gerakan Aceh Merdeka/Free Aceh Movement (GAM), while also dealing with post-disaster recovery at the same time. To assess the interaction between post-conflict recovery (PCR) and efforts of reducing future disaster risk, a framework, inspired by Wisner, et al.’s (2012) Pressure and Release (PAR) model and Barakat’s (2005) pillars of PCR is presented. The research finds that Aceh is at a lower risk of disaster compared with before the 2004 Indian Ocean tsunami. To some extent, PCR efforts have contributed to addressing people’s vulnerability and enhancing individuals’ capacities, which ultimately lead to reducing disaster risk. These improvements are due to the increased recognition and awareness of disaster issues as well as the re-establishment of governance systems in the region following the end of conflict. Nonetheless, some obstacles in achieving optimal results of PCR recovery remain. Identifying and understanding these concerns would be helpful in the pursuit of significantly reduced disaster risk through PCR process in conflict-and disaster-prone areas.

Clinical Applications of PCR

Clinical Applications of PCR Book
Author : Y. M. Dennis Lo
Publisher : Springer Science & Business Media
Release : 1998
ISBN : 1592596002
Language : En, Es, Fr & De

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Book Description :

Clinical Applications of PCR offers an unprecedented collection of core PCR techniques for the study and diagnosis of human diseases. Cutting-edge and essential for today's diagnostic laboratories, these techniques heavily utilize nonisotopic, solution phase, and in situ amplification methods. A significant number of chapters describe applications exploiting the exquisite sensitivity of PCR in the detection of rare or single cells, as in identifying fetal cells circulating in maternal blood, preimplantation embryo diagnosis, or detecting circulating cancer cells. The methods described in Clinical Applications of PCR will well serve diverse clinical specialties ranging from hematology/oncology, human genetics, and microbiology, to virology, pathology, and infectious diseases. The book repeatedly demonstrates the power of PCR-its high sensitivity, specificity, and ability to rapidly discriminate sequence variations.

PCR Primer Design

PCR Primer Design Book
Author : Anton Yuryev
Publisher : Springer Science & Business Media
Release : 2007
ISBN : 1597455288
Language : En, Es, Fr & De

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Book Description :

In the past decade, molecular biology has been transformed from the art of cloning a single gene to a statistical science measuring and calculating properties of entire genomes. New high-throughput methods have been developed for genome sequencing and studying the cell at different systematic levels such as transcriptome, proteome, metabolome and other -omes. At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). PCR Primer Design focuses on primer design, which is critical to both the efficiency and the accuracy of the PCR. With intricate descriptions of basic approaches as well as specialized methods, this volume is an exceptional reference for all those involved in studying the genome. In PCR Primer Design, authors describe basic approaches for PCR primer design in addition to specialized methods. These state-of-the-art methods can be used for both genome-scale experiments and for small-scale individual PCR amplifications. This volume will be useful for organizations performing whole genome studies, companies designing instruments that utilize PCR, and individual scientists ??? geneticists, molecular biologists, molecular geneticists, and more ??? who routinely use PCR in their research.

PCR Detection of Microbial Pathogens

PCR Detection of Microbial Pathogens Book
Author : Konrad Sachse,Joachim Frey
Publisher : Springer Science & Business Media
Release : 2003
ISBN : 1588290492
Language : En, Es, Fr & De

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Book Description :

The polymerase chain reaction has become an increasingly important tool in microbial diagnosis in recent years, mainly because of its rapidity, high sensitivity, and specificity. This book provides microbiologists and biochemists state-of-the-art protocols for the use of PCR-based identification and detection of important bacterial and other microbial pathogens. The chapters have been contributed by leading investigators well-conversant with PCR, who also offer practical expertise to the important area of pre-amplification processing. All micrbiologists, biochemists, and microbial researchers will find this to be a valuable reference for their PCR diagnosis of microbial pathogens.

PCR Protocols

PCR Protocols Book
Author : John M. S. Bartlett,David Stirling
Publisher : Springer
Release : 2011-03
ISBN : 9781617371110
Language : En, Es, Fr & De

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Book Description :

In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.