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Understanding PCR

Understanding PCR Book
Author : Sarah Maddocks,Rowena Jenkins
Publisher : Academic Press
Release : 2016-10-27
ISBN : 0128026979
Language : En, Es, Fr & De

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Book Description :

Understanding PCR: A Practical Bench-Top Guide gives you all of the information you need to plan your first PCR, from reagents to conditions to analysis and beyond. It is a user friendly book that has step-by-step basic protocols, which can be adapted to your needs. Includes helpful information such as where to order your reagents and basic troubleshooting hints and tips. Includes resources for reagents Explains basic laboratory preparation Provides straightforward experimental protocols Incorporates fundamental analytical techniques Contains a troubleshooting guide

PCR

PCR Book
Author : MCPHERSON.,S. G. Møller
Publisher : Garland Science
Release : 2000
ISBN : 1859960170
Language : En, Es, Fr & De

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Book Description :

A thoroughly updated version of the successful first edition, with a new chapter on Real-Time PCR, more prokaryotic applications, and more detail in the complex mutagenesis sections.

PCR

PCR Book
Author : Mike McPherson,Simon Møller
Publisher : Garland Science
Release : 2007-01-25
ISBN : 0203002679
Language : En, Es, Fr & De

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Book Description :

A thoroughly updated version of the successful first edition with a new chapter on Real-Time PCR, more prokaryotic applications, and more detail in the complex mutagenesis sections. Information on PCR applications in genomics and proteomics have been expanded and integrated throughout the text. There is also advice on available products and specific pointers to the most appropriate methods. As with the first edition, this will be an ideal practical introduction and invaluable guide to PCR and its applications.

Understanding Viruses

Understanding Viruses Book
Author : Teri Shors
Publisher : Jones & Bartlett Publishers
Release : 2011-11-08
ISBN : 1449677541
Language : En, Es, Fr & De

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Book Description :

The ideal text for undergraduate students majoring in biology, microbiology, medical technology, or pre-med, the Second Edition of Understanding Viruses provides a balanced approach to this fascinating discipline, combining the molecular, clinical, and historical aspects of virology. Updated throughout to keep pace with this fast-paced field, the text provides a strong, comprehensive introduction to human viral diseases. New material on molecular virology as well as new virus families presented coupled with chapters on viral diseases of animals; the history of clinical trials, gene therapy, and xenotranplantation; prions and viroids; plant viruses; and bacteriophages add to the scope of the text. Chapters discussing specific viral diseases weave in an epidemiological and global perspective and include treatment and prevention information. Contemporary case studies, Refresher Boxes, and Virus Files engage students in the learning process. With a wealth of student and instructor support tools, Understanding Viruses is an accessible, exciting, and engaging text for your virology course.

PCR Strategies

PCR Strategies Book
Author : Michael A. Innis,David H. Gelfand,John J. Sninsky
Publisher : Elsevier
Release : 1995-07-06
ISBN : 0080538541
Language : En, Es, Fr & De

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Book Description :

PCR Strategies expands and updates the landmark volume PCR Protocols. It is a companion laboratory manual that provides a completely new set of up-to-date strategies and protocols for getting the most from PCR. The editors have organized the book into four sections, focusing on principles, analyses, research applications, and alternative strategies for a wide variety of basic and clinical needs. If you own PCR Protocols, you will want PCR Strategies. If you don't own PCR Protocols, you will want to buy both! Concepts explained Methods detailed Trouble-shooting emphasized Novel applications highlighted Key concepts for PCR Analysis of PCR products Research applications Alternative amplification strategies

PCR Protocols

PCR Protocols Book
Author : John M. S. Bartlett,David Stirling
Publisher : Springer Science & Business Media
Release : 2008-02-03
ISBN : 1592593844
Language : En, Es, Fr & De

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Book Description :

In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.

Gene Quantification

Gene Quantification Book
Author : Francois Ferre
Publisher : Springer Science & Business Media
Release : 2012-12-06
ISBN : 1461241642
Language : En, Es, Fr & De

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Book Description :

Geneticists and molecular biologists have been interested in quantifying genes and their products for many years and for various reasons (Bishop, 1974). Early molecular methods were based on molecular hybridization, and were devised shortly after Marmur and Doty (1961) first showed that denaturation of the double helix could be reversed - that the process of molecular reassociation was exquisitely sequence dependent. Gillespie and Spiegelman (1965) developed a way of using the method to titrate the number of copies of a probe within a target sequence in which the target sequence was fixed to a membrane support prior to hybridization with the probe - typically a RNA. Thus, this was a precursor to many of the methods still in use, and indeed under development, today. Early examples of the application of these methods included the measurement of the copy numbers in gene families such as the ribosomal genes and the immunoglo bulin family. Amplification of genes in tumors and in response to drug treatment was discovered by this method. In the same period, methods were invented for estimating gene num bers based on the kinetics of the reassociation process - the so-called Cot analysis. This method, which exploits the dependence of the rate of reassociation on the concentration of the two strands, revealed the presence of repeated sequences in the DNA of higher eukaryotes (Britten and Kohne, 1968). An adaptation to RNA, Rot analysis (Melli and Bishop, 1969), was used to measure the abundance of RNAs in a mixed population.

Principles and Technical Aspects of PCR Amplification

Principles and Technical Aspects of PCR Amplification Book
Author : Elizabeth van Pelt-Verkuil,Alex van Belkum,John P. Hays
Publisher : Springer Science & Business Media
Release : 2008-03-14
ISBN : 1402062419
Language : En, Es, Fr & De

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Book Description :

Kary Mullis was awarded a Nobel Prize for inventing the PCR technique more than a decade ago in 1993. Since its "discovery", multiple adaptations and variations of the standard PCR technique have been described. This publication aims to provide the reader with a guide to the standard PCR technique and its many available variants, with particular emphasis being placed on the role of these PCR techniques in the clinical diagnostic laboratory (the central theme of this book).

Understanding Genetics

Understanding Genetics Book
Author : Genetic Alliance,New York-Mid-Atlantic Consortium for Genetic and Newborn Screening Services
Publisher : Lulu.com
Release : 2009
ISBN : 0982162219
Language : En, Es, Fr & De

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Book Description :

The purpose of this manual is to provide an educational genetics resource for individuals, families, and health professionals in the New York - Mid-Atlantic region and increase awareness of specialty care in genetics. The manual begins with a basic introduction to genetics concepts, followed by a description of the different types and applications of genetic tests. It also provides information about diagnosis of genetic disease, family history, newborn screening, and genetic counseling. Resources are included to assist in patient care, patient and professional education, and identification of specialty genetics services within the New York - Mid-Atlantic region. At the end of each section, a list of references is provided for additional information. Appendices can be copied for reference and offered to patients. These take-home resources are critical to helping both providers and patients understand some of the basic concepts and applications of genetics and genomics.

Clinical Applications of PCR

Clinical Applications of PCR Book
Author : Y. M. Dennis Lo
Publisher : Springer Science & Business Media
Release : 1998
ISBN : 1592596002
Language : En, Es, Fr & De

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Book Description :

Clinical Applications of PCR offers an unprecedented collection of core PCR techniques for the study and diagnosis of human diseases. Cutting-edge and essential for today's diagnostic laboratories, these techniques heavily utilize nonisotopic, solution phase, and in situ amplification methods. A significant number of chapters describe applications exploiting the exquisite sensitivity of PCR in the detection of rare or single cells, as in identifying fetal cells circulating in maternal blood, preimplantation embryo diagnosis, or detecting circulating cancer cells. The methods described in Clinical Applications of PCR will well serve diverse clinical specialties ranging from hematology/oncology, human genetics, and microbiology, to virology, pathology, and infectious diseases. The book repeatedly demonstrates the power of PCR-its high sensitivity, specificity, and ability to rapidly discriminate sequence variations.

PCR Protocols in Molecular Toxicology

PCR Protocols in Molecular Toxicology Book
Author : John P. Vanden Heuvel
Publisher : CRC Press
Release : 2019-05-07
ISBN : 9781439805800
Language : En, Es, Fr & De

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Book Description :

Molecular toxicology is an emerging discipline that utilizes molecular and cell biology to understand how drugs and chemicals result in their unwanted effects. PCR Protocols in Molecular Toxicology is a practical guide to the use of polymerase chain reaction (PCR) to help examine, on a molecular and cellular level, how toxic responses are manifested. It offers a basic understanding of PCR and its optimization, as well as describing specific, high-impact areas of molecular toxicology and recent advances. The following techniques are described in detail: Quantitative reverse transcriptase PCR and methods to examine gene expression Differential display cloning Cloning and library screening by PCR Genotype and polymorphism analysis of drug and toxicant metabolizing enzymes Basic, non-PCR based molecular biology methods PCR Protocols in Molecular Toxicology will aid both novices and experienced PCR practitioners in using PCR to its fullest potential.

Essentials of Nucleic Acid Analysis

Essentials of Nucleic Acid Analysis Book
Author : Jacquie T. Keer,Lyndsey Birch
Publisher : Royal Society of Chemistry
Release : 2008
ISBN : 0854043675
Language : En, Es, Fr & De

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Book Description :

An indispensable handbook of the highest standard for those working in the fields of food analysis and forensic applications.

Advances in Understanding Kingella kingae

Advances in Understanding Kingella kingae Book
Author : Joseph W. St. Geme, III
Publisher : Springer
Release : 2016-08-26
ISBN : 3319437291
Language : En, Es, Fr & De

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Book Description :

This book describes the growing body of information on the epidemiology, clinical manifestations, transmission, pathogenesis, diagnosis, and treatment of Kingella kingae infections in young children. In addition, it covers experimental methods that have been developed to study the microbiology, genetics, and virulence factors of K. kingae, information that provides the foundation for new approaches to treatment and prevention of K. kingae disease. With this content in mind, excerpts from the book will be of relevance for clinicians who care for pediatric patients, for clinical microbiologists who are involved in detecting organisms in clinical specimens, and for scientists who are studying K. kingae in an effort to develop novel targets for antimicrobial therapy and new approaches to prevention. First isolated in the 1960s by Elizabeth O. King, a bacteriologist at the CDC, Kingella kingae was largely ignored over the next two decades as a human pathogen because of its uncommon recovery from patients with disease. However, in recent years K. kingae has been increasingly recognized as a clinically important pathogen in young children, and is currently recognized as the leading cause of osteoarticular infections in young children in a growing number of countries. Research into this organism has grown tremendously over the past 15 years, resulting in a better appreciation of the importance of K. kingae in pediatric patients and of the molecular mechanisms of disease.

PCR Protocols in Molecular Toxicology

PCR Protocols in Molecular Toxicology Book
Author : John P. Vanden Heuvel
Publisher : CRC Press
Release : 2019-05-07
ISBN : 0429526091
Language : En, Es, Fr & De

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Book Description :

Molecular toxicology is an emerging discipline that utilizes molecular and cell biology to understand how drugs and chemicals result in their unwanted effects. PCR Protocols in Molecular Toxicology is a practical guide to the use of polymerase chain reaction (PCR) to help examine, on a molecular and cellular level, how toxic responses are manifested. It offers a basic understanding of PCR and its optimization, as well as describing specific, high-impact areas of molecular toxicology and recent advances. The following techniques are described in detail: Quantitative reverse transcriptase PCR and methods to examine gene expression Differential display cloning Cloning and library screening by PCR Genotype and polymorphism analysis of drug and toxicant metabolizing enzymes Basic, non-PCR based molecular biology methods PCR Protocols in Molecular Toxicology will aid both novices and experienced PCR practitioners in using PCR to its fullest potential.

PCR Detection of Microbial Pathogens

PCR Detection of Microbial Pathogens Book
Author : Konrad Sachse,Joachim Frey
Publisher : Springer Science & Business Media
Release : 2003
ISBN : 1588290492
Language : En, Es, Fr & De

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Book Description :

Hands-on laboratory experts present a set of "classic" PCR-based methods for the identification and detection of important animal and food microbial pathogens, including several zoonotic agents. These proven techniques can be precisely applied to a wide variety of microbes, among them Campylobacter spp., chlamydiae, toxigenic clostridia, Escherichia coli (STEC), Listeria monocytogenes, mycoplasmas, salmonellae, and Yersinia enterocolitica. Additional chapters review the specificity and performance of diagnostic PCR analysis, the pre-PCR processing of samples, the critical aspects of standardizing PCR methods, and the general issues involved in using PCR technology for microbial diagnosis.

The PCR Revolution

The PCR Revolution Book
Author : Stephen A. Bustin
Publisher : Cambridge University Press
Release : 2010
ISBN : 0521882311
Language : En, Es, Fr & De

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Book Description :

Examines the latest innovations and the overall impact of PCR on areas of molecular research.

Basic Science Methods for Clinical Researchers

Basic Science Methods for Clinical Researchers Book
Author : Morteza Jalali,Francesca Yvonne Louise Saldanha,Mehdi Jalali
Publisher : Academic Press
Release : 2017-03-31
ISBN : 012803078X
Language : En, Es, Fr & De

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Book Description :

Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow’s clinician scientists and future leaders in discovery science. Serves as a helpful guide for clinical researchers who lack a conventional science background Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)

Molecular Biology of the Cell

Molecular Biology of the Cell Book
Author : Bruce Alberts
Publisher : Unknown
Release : 2004
ISBN : 9780815332183
Language : En, Es, Fr & De

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Book Description :

Download Molecular Biology of the Cell book written by Bruce Alberts, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Basic Molecular Protocols in Neuroscience Tips Tricks and Pitfalls

Basic Molecular Protocols in Neuroscience  Tips  Tricks  and Pitfalls Book
Author : John T. Corthell
Publisher : Academic Press
Release : 2014-04-16
ISBN : 0128015276
Language : En, Es, Fr & De

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Book Description :

Basic Neuroscience Protocols: Tips, Tricks, and Pitfalls contains explanatory sections that describe the techniques and what each technique really tells the researcher on a scientific level. These explanations describe relevant controls, troubleshooting, and reaction components for some of the most widely used neuroscience protocols that remain difficult for many neuroscientists to implement successfully. Having this additional information will help researchers ensure that their experiments work the first time, and will also minimize the time spent working on a technique only to discover that the problem was them, and not their materials. Describes techniques in very specific detail with step-by-step instructions, giving researchers in-depth understanding Offers many details not present in other protocol books Describes relevant controls for each technique and what those controls mean Chapters include references (key articles, books, protocols) for additional study Describes both the techniques and the habits necessary to get quality results, such as aseptic technique, aliquoting, and general laboratory rules

PCR Protocols

PCR Protocols Book
Author : Michael A. Innis,David H. Gelfand,John J. Sninsky,Thomas J. White
Publisher : Academic Press
Release : 2012-12-02
ISBN : 008088671X
Language : En, Es, Fr & De

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Book Description :

The correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive manual. Avoid contamination--with specific instructions on setting up your lab Avoid cumbersome molecular biological techniques Discover new applications