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Genetics Classical to Modern

Genetics  Classical to Modern Book
Author : P. K. Gupta
Publisher : Unknown
Release : 2014-05-14
ISBN : 9781441672100
Language : En, Es, Fr & De

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Book Description :

1. Genetics, Epigenetics and Genomics: An Overview 2. Mendel's Laws of Inheritance 3. Lethality and Interaction of Genes 4. Genetics of Quantitative Traits (QTs): 1. Mendelian Approach (Multiple Factor Hypothesis) 5. Genetics of Quantitative Traits: 2. Biometrical Approach 6. Genetics of Quantitative Traits: 3. Molecular Markers and QTL Analysis 7. Genetics of Quantitative Traits: 4. Linkage Disequilibrium (LD) and Association Mapping 8. Multiple Alleles and Isoalleles 9. Physical Basis of Heredity 1. The Chromosome Theory of Inheritance 10. Physical Basis of Heredity 2. The Nucleus and the Chromosome 11. Physical Basis of Heredity 3. Cell Division (Mitosis and Meiosis) 12. The Cell Division Cycle: Molecular Basis 13. Linkage and Crossing Over in Diploid Organisms (Higher Eukaryotes) 14. Tetrad Analysis, Mitotic Recombination and Gene Conversion in Haploid Organisms (Fungi and Single Celled Algae) 15. Genetics of Sexuality and Recombination in Bacteria and Viruses 16. Molecular Basis of Division of Bacterial Cells and Eukaryotic Organelles (Including Sporulation in Bacteria) 17. Molecular Basis of Homologous Recombination (HR) 18. Molecular Basis of Site-Specific Recombination (Gene Targeting) 19. Recombination and Resolution of Gene Structure (A Modified Concept of Allelomorphism) 20. Accessory Genetic Elements: Plasmids, Transposons and Retroelements 21. Sex-Linked, Sex-Influenced and Sex-Limited Traits (Including Sex-Biased Inheritance) 22. Genetics of Sex Determination, Sex Differentiation and Dosage Compensation 23. Maternal Effects and Cytoplasmic Inheritance 24. Structural Changes In Chromosomes 25. Numerical Changes in Chromosomes 26. Mutations: 1. Morphological Level (Including Lethal Mutations) 27. Mutations: 2. Biochemical Level (Biochemical and Microbial Genetics) 28. Mutations: 3. Molecular Mechanism and Use m Functional Genomics 29. Human Genetics and Genomics 30. Chemistry of the Gene 1. Nucleic Acids and Their Structure 31. Chemistry of the Gene 2. Synthesis, Modification and Repair of DNA 32. Organization of Genetic Material 1. Genome Size, C- Value Paradox and Repetitive DNA Sequences 33. Organisation of Genetic Material 2. Packaging of DNA as Nucleosomes ID Eukaryotes 34. Organization of Genetic Material 3. Mitochondrial and Chloroplast Genomes 35. Organization of Genetic Material 4. Split Genes, Overlapping Genes, Pseudogenes, Retrogenes and Cryptic Genes 36. The Genetic Code 37. Expression of Gene and Protein Synthesis 1. Transcription in Prokaryotes and Eukaryotes 38. Expression of Gene and Protein Synthesis: 2. RNA Processing 39. Expression of Gene and Protein Synthesis: 3. Protein Structure and Molecular Machines for Translation of mRNA (Ribosome, tRNA and aaRS) 40. Expression of Gene and Protein Synthesis: 4. Translation of mRNA III Prokaryotes and Eukaryotes 41. Protein Modification, Folding, Translocation and Degradation 42. Regulation of Gene Expression 1. Operon Circuits in Bacteria and Other Prokaryotes 43. Regulation of Gene Expression 2. Regulation Cascades in Bacteriophages 44. Regulation of Gene Expression 3. A Variety of Mechanisms m Eukaryotes 45. Regulation of Gene Expression 4. Chromatin Remodeling and Cellular Memory 46. Cell Receptors and Signal Transduction 47. Genes in Development 48. Behavioural Genetics 49. Epigenetics and Epigenomics 50. Genetic Engineering and Biotechnology 1. Recombinant DNA, Molecular Probes, Gene Libraries, PCR (Cloning and Amplification of DNA) and DNA Chips 51. Genetic Engineering and Biotechnology 2. Restriction Maps and Molecular Marker Maps 52. Genetic Engineering and Biotechnology 3. Isolation, Sequencing and Synthesis of Genes 53. Genetic Engineering and Biotechnology 4. Gene Transfer Methods and Transgenic Organisms 54. Genetic Engineering and Biotechnology 5. Hybridoma and Monoclonal Antibodies 55. Multigene Families In Eukaryotes 56. Genomics and Proteomics (Animals, Plants and Microbes) 57. Genetics of Cancer: Proto-oncogenes, Oncogenes

The Gene

The Gene Book
Author : Siddhartha Mukherjee
Publisher : Simon and Schuster
Release : 2016
ISBN : 147673352X
Language : En, Es, Fr & De

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Book Description :

Prologue: Families -- "The missing science of heredity" 1865-1935 -- "In the sum of the parts, there are only the parts" 1930-1970 -- "The dreams of geneticists" 1970-2001 -- "The proper study of mankind is man" 1970-2005 -- Through the looking glass 2001-2015 -- Post-genome 2015- ... -- Epilogue: Bheda, Abheda

Evolution and the Genetics of Populations Volume 2

Evolution and the Genetics of Populations  Volume 2 Book
Author : Sewall Wright
Publisher : University of Chicago Press
Release : 1984-06-15
ISBN : 9780226910390
Language : En, Es, Fr & De

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Book Description :

These volumes discuss evolutionary biology through the lense of population genetics.

Super Gene 2 Anthology

Super Gene 2 Anthology Book
Author : Twelve-Winged Dark Seraphim
Publisher : BEIJING BOOK CO. INC.
Release : 101-01-01
ISBN : 7999092150
Language : En, Es, Fr & De

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Book Description :

In the magnificent interstellar era, mankind has finally developed teleport technology, but when trying to teleport, they are not sent to the future, the past, or any land known to men... This mysterious space is called God¡¯s Sanctuary, where lived numerous unknown creatures. Here, humans will make the greatest leap in their evolution to create the most glorious epoch in history. "Sacred-blood creature black beetle killed. Beast soul of sacred-blood black beetle gained. Eat the meat of sacred-blood black beetle to gain 0 to 10 geno points randomly."

Neurofibromatosis 2

Neurofibromatosis 2 Book
Author : Markku Sainio
Publisher : Unknown
Release : 2000
ISBN : 9789514591228
Language : En, Es, Fr & De

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Book Description :

Download Neurofibromatosis 2 book written by Markku Sainio, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Bacteriophage M13 gene 2 protein and its function in DNA replication

Bacteriophage M13 gene 2 protein and its function in DNA replication Book
Author : Shin-chsiang Lin
Publisher : Unknown
Release : 1973
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Bacteriophage M13 gene 2 protein and its function in DNA replication book written by Shin-chsiang Lin, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Neurofibromatosis 2

Neurofibromatosis 2 Book
Author : Markku Sainio
Publisher : Unknown
Release : 2000
ISBN : 9789529117680
Language : En, Es, Fr & De

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Book Description :

Download Neurofibromatosis 2 book written by Markku Sainio, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Alien Gene Transfer in Crop Plants Volume 2

Alien Gene Transfer in Crop Plants  Volume 2 Book
Author : Aditya Pratap,Jitendra Kumar
Publisher : Springer Science & Business Media
Release : 2014-02-03
ISBN : 1461495725
Language : En, Es, Fr & De

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Book Description :

Genetic engineering and biotechnology along with conventional breeding have played an important role in developing superior cultivars by transferring economically important traits from distant, wild and even unrelated species to the cultivated varieties which otherwise could not have been possible with conventional breeding. There is a vast amount of literature pertaining to the genetic improvement of crops over last few decades. However, the wonderful results achieved by crop scientists in food legumes’ research and development over the years are scattered in different journals of the World. The two volumes in the series ‘Alien Gene Transfer in Crop Plants’ address this issue and offer a comprehensive reference on the developments made in major food crops of the world. These volumes aim at bringing the contributions from globally renowned scientists at one platform in a reader-friendly manner. The second volume entitled, “Alien Gene Transfer in Crop Plants: Achievements and Impact” will deal more with the practical aspects. This volume will cover achievements of alien gene transfer in major food crops of the world and their impact on development of newer genetic variability and additional avenues for selection; development of superior cultivars for increased yield, resistance to biotic and abiotic stresses, improved nutritional and industrial quality; innovation of new techniques and positive as well as negative environmental implications. This volume has been divided into four groups with an aim to cover all major cereals, pulses, oilseeds and other crops (vegetable and horticultural crops) which are of economic importance.

Molecular Biology and Genetic Engineering

Molecular Biology and Genetic Engineering Book
Author : P. K. Gupta
Publisher : Rastogi Publications
Release : 2008
ISBN : 9788171337194
Language : En, Es, Fr & De

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Book Description :

PART I Molecular Biology 1. Molecular Biology and Genetic Engineering Definition, History and Scope 2. Chemistry of the Cell: 1. Micromolecules (Sugars, Fatty Acids, Amino Acids, Nucleotides and Lipids) Sugars (Carbohydrates) 3. Chemistry of the Cell . 2. Macromolecules (Nucleic Acids; Proteins and Polysaccharides) Covalent and Weak Non-covalent Bonds 4. Chemistry of the Gene: Synthesis, Modification and Repair of DNA DNA Replication: General Features 5. Organisation of Genetic Material 1. Packaging of DNA as Nucleosomes in Eukaryotes Techniques Leading to Nucleosome Discovery 6. Organization of Genetic Material 2. Repetitive and Unique DNA Sequences 7. Organization of Genetic Material: 3. Split Genes, Overlapping Genes, Pseudogenes and Cryptic Genes Split Genes or .Interrupted Genes 8. Multigene Families in Eukaryotes 9. Organization of Mitochondrial and Chloroplast Genomes 10. The Genetic Code 11. Protein Synthesis Apparatus Ribosome, Transfer RNA and Aminoacyl-tRNA Synthetases Ribosome 12. Expression of Gene . Protein Synthesis 1. Transcription in Prokaryotes and Eukaryotes 13. Expression of Gene: Protein Synthesis: 2. RNA Processing (RNA Splicing, RNA Editing and Ribozymes) Polyadenylation of mRNA in Prokaryotes Addition of Cap (m7G) and Tail (Poly A) for mRNA in Eukaryotes 14. Expression of Gene: Protein Synthesis: 3. Synthesis and Transport of Proteins (Prokaryotes and Eukaryotes) Formation of Aminoacyl tRNA 15. Regulation of Gene Expression: 1. Operon Circuits in Bacteria and Other Prokaryotes 16. Regulation of Gene Expression . 2. Circuits for Lytic Cycle and Lysogeny in Bacteriophages 17. Regulation of Gene Expression 3. A Variety of Mechanisms in Eukaryotes (Including Cell Receptors and Cell Signalling) PART II Genetic Engineering 18. Recombinant DNA and Gene Cloning 1. Cloning and Expression Vectors 19. Recombinant DNA and Gene Cloning 2. Chimeric DNA, Molecular Probes and Gene Libraries 20. Polymerase Chain Reaction (PCR) and Gene Amplification 21. Isolation, Sequencing and Synthesis of Genes 22. Proteins: Separation, Purification and Identification 23. Immunotechnology 1. B-Cells, Antibodies, Interferons and Vaccines 24. Immunotechnology 2. T-Cell Receptors and MHC Restriction 25. Immunotechnology 3. Hybridoma and Monoclonal Antibodies (mAbs) Hybridoma Technology and the Production of Monoclonal Antibodies 26. Transfection Methods and Transgenic Animals 27. Animal and Human Genomics: Molecular Maps and Genome Sequences Molecular Markers 28. Biotechnology in Medicine: l.Vaccines, Diagnostics and Forensics Animal and Human Health Care 29. Biotechnology in Medicine 2. Gene Therapy Human Diseases Targeted for Gene Therapy Vectors and Other Delivery Systems for Gene Therapy 30. Biotechnology in Medicine: 3. Pharmacogenetics / Pharmacogenomics and Personalized Medicine Phannacogenetics and Personalized 31. Plant Cell and Tissue Culture' Production and Uses of Haploids 32. Gene Transfer Methods in Plants 33. Transgenic Plants . Genetically Modified (GM) Crops and Floricultural Plants 34. Plant Genomics: 35. Genetically Engineered Microbes (GEMs) and Microbial Genomics References

The Genetic Approach to Human Disease

The Genetic Approach to Human Disease Book
Author : Vincent M. Riccardi
Publisher : Oxford University Press, USA
Release : 1977
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download The Genetic Approach to Human Disease book written by Vincent M. Riccardi, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Proceedings of the National Academy of Sciences of the United States of America

Proceedings of the National Academy of Sciences of the United States of America Book
Author : National Academy of Sciences (U.S.)
Publisher : Unknown
Release : 2006
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Proceedings of the National Academy of Sciences of the United States of America book written by National Academy of Sciences (U.S.), available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Soviet Genetics

Soviet Genetics Book
Author : Anonim
Publisher : Unknown
Release : 1980
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Soviet Genetics book written by , available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

The Genetics of Type 2 Diabetes and Related Traits

The Genetics of Type 2 Diabetes and Related Traits Book
Author : Jose C. Florez
Publisher : Springer
Release : 2016-04-11
ISBN : 3319015745
Language : En, Es, Fr & De

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Book Description :

This book presents the state of the art of type 2 diabetes genetics, from the process of genetic discovery to its interpretation and clinical application, and illustrates a model for other complex human phenotypes.The first section explores genome-wide association studies, the extension of this method to less accessible phenotypes and the arrival of next-generation sequencing. A further section goes beyond genetics to illustrate how other data sources can help interpret genetic data, such as leveraging population diversity, the correlation of genetic associations with physiological measurements, gene expression modulation, environmental factors and our microbial commensals. The third section describes advances in elucidating the complex path from association to function using in-depth sequencing and functional studies of the cellular and molecular effects of genes in the loci identified by genetics. The final section links our current understanding with clinically relevant questions, such as prediction, interactions with drugs or nutrients, and disease prevention, and paints a realistic but hopeful vision of the future. ​

Concepts of Genetics

Concepts of Genetics Book
Author : William S. Klug,Michael R. Cummings
Publisher : Unknown
Release : 2003
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

For one- or two-term courses in Genetics in the departments of Biology, Zoology, Agriculture or Health Science. This text is known for its clear writing style, emphasis on concepts, visual art program and thoughtful coverage of all areas of genetics. The authors capture students' interest with up-to-date coverage of cutting-edge topics and research. The authors emphasize those concepts that students should come to understand and take away with them, not a myriad of details and exceptions that need to memorized and are soon forgotten. The seventh edition boasts the next generation of interactive learning tutorials and animations integrated within the Companion Website.

Genomics of Plant Genetic Resources

Genomics of Plant Genetic Resources Book
Author : Roberto Tuberosa,Andreas Graner,Emile Frison
Publisher : Springer Science & Business Media
Release : 2013-12-23
ISBN : 940077575X
Language : En, Es, Fr & De

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Book Description :

Our lives and well being intimately depend on the exploitation of the plant genetic resources available to our breeding programs. Therefore, more extensive exploration and effective exploitation of plant genetic resources are essential prerequisites for the release of improved cultivars. Accordingly, the remarkable progress in genomics approaches and more recently in sequencing and bioinformatics offers unprecedented opportunities for mining germplasm collections, mapping and cloning loci of interest, identifying novel alleles and deploying them for breeding purposes. This book collects 48 highly interdisciplinary articles describing how genomics improves our capacity to characterize and harness natural and artificially induced variation in order to boost crop productivity and provide consumers with high-quality food. This book will be an invaluable reference for all those interested in managing, mining and harnessing the genetic richness of plant genetic resources.

Automated Design and Optimization of Wire Antennas Using Genetic Algorithms

Automated Design and Optimization of Wire Antennas Using Genetic Algorithms Book
Author : Derek Scott Linden
Publisher : Unknown
Release : 1997
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

A Genetic Algorithm (GA) has been used in conjunction with the Numerical Electromagnetics Code, Version 2 (NEC2) to create and optimize atypical wire antenna designs with impressive characteristics. Antenna design parameters are encoded into an ordered series of numbers and/or symbols analogous to a biological chromosome. A cost function that quantifies how well a design meets the engineer's specifications is created. The GA uses these to generate and evaluate a population of designs. The most successful designs are then promoted and mixed through mating and mutation, while poor designs are removed. This process, difficult to trap in local minima, continues until convergence criteria are met, generally yielding excellent designs with no user intervention or initial guesses. Three antennas have been optimized: a monopole loaded with a modified folded dipole, the Yagi antenna, and the crooked-wire genetic antenna. Prior study of the loaded monopole had shown hemispherical coverage was possible. The GA found an asymmetric loaded monopole with an average variation in gain over the hemisphere of only 0.4dB, confirmed by measurement. GA-optimized Yagi antennas surpassed the gain of conventional Yagis by about 1dB, improvement also confirmed by measurement. The GA designed a Yagi with a beamwidth of 50 deg -60 deg, sidelobes nearly 25dB down, and a 14% bandwidth-specifications difficult to achieve using conventional techniques. The crooked-wire genetic antenna is several wires joined in series; locations and lengths are determined by the GA. Optimization for hemispherical coverage with right-hand circular polarization (RHCP) produced highly unusual shapes unrealizable using a conventional approach. RHCP hemispherical coverage was achieved with less than 4dB variation. Measurements verify the results.

Genetics and the Behavior of Domestic Animals

Genetics and the Behavior of Domestic Animals Book
Author : Per Jensen,Dominic Wright
Publisher : Elsevier Inc. Chapters
Release : 2013-04-22
ISBN : 0128059931
Language : En, Es, Fr & De

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Book Description :

In this chapter, we begin by defining domestication as a genetic process, whereby animals change phenotypically and genetically as a response to living under human supervision. This has caused a convergent set of phenotypic modifications across numerous different species, through a combination of relaxed natural selection, increased human selection, and correlated responses. The genetic mechanisms underlying these processes are discussed in addition to genetic drift and inbreeding. The driving types of mutations are dealt with, as is the way in which genes are organized in the genome to give rise to a specific genetic architecture facilitating selection responses. The different methods used to discover genes and mutations are divided into either top-down approaches, where one starts with the phenotype and uses mapping methods to find the causative genes, or bottom-up, where the genetic polymorphism is the starting point and the phenotype the desired response variable. A number of examples of single genes and mutations that have been coupled to behavioral variation are given, among them genes showing pleiotropic effects on pigmentation, and genes involved in regulation of the effects of neuropeptides. Finally, the relatively novel area of behavioral epigenetics is examined, in particular with its possible relation to domestication effects.

Handbook of Human Molecular Evolution 2 Volume Set

Handbook of Human Molecular Evolution  2 Volume Set Book
Author : David N. Cooper,Hildegard Kehrer-Sawatzki
Publisher : Wiley
Release : 2008-10-13
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

"This splendid compendium ... will be the standard reference work for years to come: a handbook to browse, to consult, to look things up in, and to read with pleasure, wonder and post-Darwinian exhilaration." —Richard Dawkins "This is a marvellous book… It should be in every university library - preferably in several copies - and every reader of this journal should add it to their next grant application. It really is that good... I have already found this book to be invaluable… For many years to come, these two volumes will be the starting point for anyone wishing to find out about virtually any subject relating to human genetics… Any scientist working on humans or other animals will find many things in these pages that will stimulate, inform and inspire. The authors, editors and publishers are to be congratulated for their work… order a copy now!" —HUMAN GENETICS "The publishers and editors deserve to be congratulated for publishing this major book which coincides with the 200th anniversary of the birth of Charles Darwin. The book is well-timed, with biologists, theologians and sociologists engaged in intense debate on the Darwinian Theory on the origin of species, evolution and natural selection… There is little doubt that this marvellous publication should be in the library of universities and academic institutions dealing with basic and applied biology research and education… It will not be surprising if the individual academic or researcher decides to invest in this resource and enrich their personal collection of leading books in genetics and genomics." —GENOMIC MEDICINE A Unique Collection of High-Quality Articles – Derived from the Acclaimed Encyclopedia of Life Sciences The revolution in human molecular genetics which has taken place over the last three decades has yielded a wealth of information not only on the structure and function of our genes, but also on gene expression, mutation and polymorphic variation. Over the last five years, the focus has moved from genes to genomes. Even though the annotation of our ~30,000 genes is still in progress, genome-wide studies have already yielded abundant evidence for the signatures of past selection and adaptive evolution within human gene sequences. Further, the completion of the sequencing of the 3 billion base-pair human genome, coupled with the increasing availability of other vertebrate genome sequences, has ushered in a new era of comparative genomics. We are now able to identify many of the molecular events (from the chromosomal level down to the single base-pair) that have occurred during vertebrate, mammalian, primate and hominid evolution. Indeed, the detailed comparison of the human and chimpanzee genomes has begun to reveal some of the genetic changes that have been involved in the development of human lineage-specific traits. We are thus acquiring the ability to ask searching questions about our origins, about the demographic processes associated with the global radiation of humankind, as well as some of the unique adaptations that make us human. Evolutionary biology has become so broad that its impact may be felt across the spectrum of the biological sciences. The aim of the Handbook of Human Molecular Evolution is relatively straightforward: to bring together under the same cover the many and varied strands of our knowledge of human/primate/vertebrate molecular evolution. Hence, the 282 chapters that comprise this essential reference work have been thematically arranged into twelve sections, covering the whole scope of research into human molecular evolution: General Concepts in Evolutionary Genetics Mutation, Adaptation and Natural Selection Evolutionary and Population Genetics Human Evolution Human Genome Evolution Evolution of Human Gene Structure and Function Evolution of Gene Expression Mitochondrial Genome Evolution Chromosomal Evolution Comparative Genomics Evolution and Disease Susceptibility Analysis of Ancient DNA This conceptual outline informed the selection of the chapters themselves and the connections between them. Some of these chapters are intended to be introductory, aimed at undergraduates and non-specialists. They provide basic information and a list of recommended further reading to encourage the reader to explore a topic in more depth. This approach helps the student reader progress from textbook material to primary literature. Some chapters are overviews that address topics of broad interest and importance, while others focus on quite specialized topics. These chapters are written for postgraduate students and research workers; they contain more detailed information and key references allowing the reader to investigate a specific area in more depth. This format allows professionals to use the books as a quick reference source. The chapters are richly supplied with website information to allow access to relevant data sources over the internet. The self-contained, peer-reviewed articles in this unique handbook have been written by leading scientists in each field. Key topics include the evolution of enzyme function, the use of nucleic acid divergence as a "molecular clock", the origin of non-functional or junk DNA, the role of gene duplication in the emergence of novel gene function and the identification of molecular changes responsible for various human characteristics especially those pertaining to infection, cognition, disease and disease susceptibility. The Handbook of Human Molecular Evolution has adopted an integrated approach to the study of human evolution and seeks throughout to emphasize the interplay between molecular genetic concepts and principles on the one hand, and information acquisition and interpretation on the other. In this way, it is hoped that the ‘documents of evolutionary history’ written into the fabric of our genome, will become accessible to the widest possible audience.

Molecular Biology of Dictyostelium Development

Molecular Biology of Dictyostelium Development Book
Author : Alan R. Kimmel
Publisher : Alan R. Liss
Release : 1988
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Molecular Biology of Dictyostelium Development book written by Alan R. Kimmel, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Gene Delivery to Mammalian Cells

Gene Delivery to Mammalian Cells Book
Author : William C. Heiser
Publisher : Humana Press
Release : 2010-11-10
ISBN : 9781617373534
Language : En, Es, Fr & De

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Book Description :

The efficiency of delivering DNA into mammalian cells has increased t- mendously since DEAE dextran was first shown to be capable of enhancing transfer of RNA into mammalian cells in culture. Not only have other chemical methods been developed and refined, but also very efficient physical and viral delivery methods have been established. The technique of introducing DNA into cells has developed from transfecting tissue culture cells to delivering DNA to specific cell types and organs in vivo. Moreover, two important areas of biology—assessment of gene function and gene therapy—require succe- ful DNA delivery to cells, driving the practical need to increase the efficiency and efficacy of gene transfer both in vitro and in vivo. TM These two volumes of the Methods in Molecular Biology series, Gene Del- ery to Mammalian Cells, are designed as a compendium of those techniques that have proven most useful in the expanding field of gene transfer in mammalian cells. It is intended that these volumes will provide a thorough background on chemical, physical, and viral methods of gene delivery, a synopsis of the myriad techniques currently available to introduce genes into mammalian cells, as well as a practical guide on how to accomplish this. It is my expectation that it will be useful to the novice in the field as well as to the scientist with expertise in gene delivery.