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Single Molecule Biology

Single Molecule Biology Book
Author : Alexander E. Knight
Publisher : Academic Press
Release : 2009-02-26
ISBN : 9780080921235
Language : En, Es, Fr & De

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Book Description :

Single molecule techniques, including single molecule fluorescence, optical tweezers, and scanning probe microscopy, allow for the manipulation and measurement of single biological molecules within a live cell or in culture. These approaches, amongst the most exciting tools available in biology today, offer powerful new ways to elucidate biological function, both in terms of revealing mechanisms of action on a molecular level as well as tracking the behaviour of molecules in living cells. This book provides the first complete and authoritative treatment of this rapidly emerging field, explicitly from a biological perspective. The contents are organized by biological system or molecule. Each chapter discusses insights that have been revealed about their mechanism, structure or function by single molecule techniques. Among the topics covered are enzymes, motor proteins, membrane channels, DNA, ribozymes, cytoskeletal proteins, and other key molecules of current interest. An introduction by the editor provides a concise review of key principles and an historical overview. The last section discusses applications in molecular diagnostics and drug discovery. * Organized by biological system or molecule. * Each chapter discusses insights into mechanism of action, structure, and function * Covers enzymes, motor proteins, membrane channels, DNA, ribozymes, etc. * Includes an introduction to key principles and an historical overview. * Discusses applications in molecular diagnostics and drug discovery. * Provides an expert's perspective on future developments.

Single molecule Biophysics and Molecular Biology

Single molecule Biophysics and Molecular Biology Book
Author : Anonim
Publisher : Unknown
Release : 2000
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Single molecule Biophysics and Molecular Biology book written by , available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Biology at the Single Molecule Level

Biology at the Single Molecule Level Book
Author : S.H. Leuba,J. Zlatanova
Publisher : Elsevier
Release : 2001-12-17
ISBN : 9780080560618
Language : En, Es, Fr & De

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Book Description :

This is the first book solely devoted to single-molecule biochemistry and molecular biology. Authors were selected on the basis of their contribution to this new and exciting field, and were asked to focus more on the biological problems that can be approached using single-molecule techniques rather than on the techniques per se. It is thought that such techniques will eventually dominate the physical characterization of biologically important macromolecules.

Single Molecule Biophysics

Single Molecule Biophysics Book
Author : Tamiki Komatsuzaki,Masaru Kawakami,Satoshi Takahashi,Haw Yang,Robert J. Silbey
Publisher : John Wiley & Sons
Release : 2011-11-16
ISBN : 111813138X
Language : En, Es, Fr & De

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Book Description :

Discover the experimental and theoretical developments inoptical single-molecule spectroscopy that are changing the ways wethink about molecules and atoms The Advances in Chemical Physics series provides thechemical physics field with a forum for critical, authoritativeevaluations of advances in every area of the discipline. Thislatest volume explores the advent of optical single-moleculespectroscopy, and how atomic force microscopy has empowered novelexperiments on individual biomolecules, opening up new frontiers inmolecular and cell biology and leading to new theoreticalapproaches and insights. Organized into two parts—oneexperimental, the other theoretical—this volume exploresadvances across the field of single-molecule biophysics, presentingnew perspectives on the theoretical properties of atoms andmolecules. Single-molecule experiments have provided freshperspectives on questions such as how proteins fold to specificconformations from highly heterogeneous structures, how signaltransductions take place on the molecular level, and how proteinsbehave in membranes and living cells.This volume is designed tofurther contribute to the rapid development of single-moleculebiophysics research. Filled with cutting-edge research reported in a cohesive mannernot found elsewhere in the literature, each volume of theAdvances in Chemical Physics series serves as the perfectsupplement to any advanced graduate class devoted to the study ofchemical physics.

Single Molecule Spectroscopy in Chemistry Physics and Biology

Single Molecule Spectroscopy in Chemistry  Physics and Biology Book
Author : Astrid Graslund
Publisher : Springer Science & Business Media
Release : 2008
ISBN : 3642025986
Language : En, Es, Fr & De

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Book Description :

Written by the leading experts in the field, this book describes the development and current state of the art in single molecule spectroscopy. The application of this technique, which started 1989, in physics, chemistry and biosciences is displayed.

Single Molecule Analysis

Single Molecule Analysis Book
Author : Erwin J. G. Peterman,Gijs J. L. Wuite
Publisher : Humana Press
Release : 2011-09-15
ISBN : 9781617792816
Language : En, Es, Fr & De

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Book Description :

Life scientists believe that life is driven, directed, and shaped by biomolecules working on their own or in concert. It is only in the last few decades that technological breakthroughs in sensitive fluorescence microscopy and single-molecule manipulation techniques have made it possible to observe and manipulate single biomolecules and measure their individual properties. The methodologies presented in Single Molecule Techniques: Methods and Protocols are being applied more and more to the study of biologically relevant molecules, such as DNA, DNA-binding proteins, and motor proteins, and are becoming commonplace in molecular biophysics, biochemistry, and molecular and cell biology. The aim of Single Molecule Techniques: Methods and Protocols is to provide a broad overview of single-molecule approaches applied to biomolecules on the basis of clear and concise protocols, including a solid introduction to the most widely used single-molecule techniques, such as optical tweezers, single-molecule fluorescence tools, atomic force microscopy, magnetic tweezers, and tethered particle motion. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Single Molecule Techniques: Methods and Protocols serves as an ideal guide to scientists of all backgrounds and provides a broad and thorough overview of the exciting and still-emerging field of single-molecule biology.

Single molecule Biochemistry and Molecular Biology

Single molecule Biochemistry and Molecular Biology Book
Author : Anonim
Publisher : Unknown
Release : 2001
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Single molecule Biochemistry and Molecular Biology book written by , available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Focussed Issue Single molecule Biophysics and Molecular Biology

Focussed Issue  Single molecule Biophysics and Molecular Biology Book
Author : S. Leuba
Publisher : Unknown
Release : 2000
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Focussed Issue Single molecule Biophysics and Molecular Biology book written by S. Leuba, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Single molecule Studies of Proteins

Single molecule Studies of Proteins Book
Author : Andres F. Oberhauser
Publisher : Springer Science & Business Media
Release : 2012-11-09
ISBN : 1461449219
Language : En, Es, Fr & De

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Book Description :

In Single Molecule Studies of Proteins, expert researchers discuss the successful application of single-molecule techniques to a wide range of biological events, such as the imaging and mapping of cell surface receptors, the analysis of the unfolding and folding pathways of single proteins, the analysis interaction forces between biomolecules, the study of enzyme catalysis or the visualization of molecular motors in action. The chapters are aimed at established investigators and post-doctoral researchers in the life sciences wanting to pursue research in the various areas in which single-molecule approaches are important; this volume also remains accessible to advanced graduate students seeking similar research goals.

Biophysics of DNA Protein Interactions

Biophysics of DNA Protein Interactions Book
Author : Mark C. Williams,L. James Maher, III
Publisher : Springer Science & Business Media
Release : 2010-10-05
ISBN : 9780387928081
Language : En, Es, Fr & De

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Book Description :

Depite the rapid expansion of the field of biophysics, there are very few books that comprehensively treat specific topics in this area. Recently, the field of single molecule biophysics has developed very quickly, and a few books specifically treating single molecule methods are beginning to appear. However, the promise of single molecule biophysics is to contribute to the understanding of specific fields of biology using new methods. This book would focus on the specific topic of the biophysics of DNA-protein interactions, and would include the use of new approaches, including both bulk methods as well as single molecule methods. This would make the book attractive to anyone working in the general area of DNA-protein interactions, which is of course a much wider market than just single molecule biophysicists or even biophysicists. The subject of the book will be the biophysics of DNA-protein interactions, and will include new methods and results that describe the physical mechanism by which proteins interact with DNA. For example, there has been much recent work on the mechanism by which proteins search for specific binding sites on DNA. A few chapters will be devoted to experiments and theory that shed light on this important problem. We will also cover proteins that alter DNA properties to facilitate interactions important for transcription or replication. Another section of the book will cover the biophysical mechanism by which motor proteins interact with DNA. Finally, we will cover larger protein-DNA complexes, such as replication forks, recombination complexes, DNA repair interactions, and their chromatin context.

Single molecule Techniques

Single molecule Techniques Book
Author : Paul R. Selvin,Taekjip Ha
Publisher : CSHL Press
Release : 2008
ISBN : 087969775X
Language : En, Es, Fr & De

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Book Description :

As molecular and cellular biologists move toward nano-techniques for performing experiments on single molecules rather than on populations of molecules, a comprehensive manual on how (and why) to carry out such experiments is needed. Single-Molecule Techniques: A Laboratory Manualfills this requirement – it is the first to take researchers who know nothing about single-molecule analyses to the point where they can successfully design and execute appropriate experiments. Geared toward research scientists in structural and molecular biology, biochemistry, and biophysics, the manual will be useful to all who are interested in observing, manipulating, and elucidating the molecular mechanisms and discrete properties of macromolecules. Techniques range from in vivo and in vitro fluorescent-based methods to the use of atomic force microscopy, optical and magnetic tweezers, and nanopores. The book is edited by Paul R. Selvin and Taekjip Ha, two pioneers in the field of experimental biophysics who have made significant contributions to the development and application of single-molecule technologies.

Cell Signaling Reactions

Cell Signaling Reactions Book
Author : Yasushi Sako,Masahiro Ueda
Publisher : Springer Science & Business Media
Release : 2010-11-04
ISBN : 9789048198641
Language : En, Es, Fr & De

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Book Description :

This book encompasses the exciting developments and challenges in the fast-moving and rapidly expanding research field of single-molecule kinetic analysis of cell signaling that promises to be one of the most significant and exciting areas of biological research for the foreseeable future. Cell signaling is carried out by complicated reaction networks of macromolecules, and single-molecule analyses has already demonstrated its power to unravel complex reaction dynamics in purified systems. To date, most of the published research in the field of single-molecule processes in cells, focus on the dynamic properties (translational movements of the centre of mass) of biological molecules. However, we hope that this book presents as many kinetic analyses of cell signaling as possible. Although single-molecule kinetic analysis of cellular systems is a relatively young field when compared with the analysis of single-molecule movements in cells, this type of analysis is highly important because it directly relates to the molecular functions that control cellular behavior and in the future, single-molecule kinetic analysis will be largely directed towards cellular systems. Thus, we hope that this book will be of interest to all those working in the fields of molecular and cell biology, as well as biophysics and biochemistry.

Single Molecules and Nanotechnology

Single Molecules and Nanotechnology Book
Author : Rudolf Rigler,H. Vogel
Publisher : Springer Science & Business Media
Release : 2007-12-07
ISBN : 9783540739241
Language : En, Es, Fr & De

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Book Description :

This book focuses on recent advances in the rapidly evolving field of single molecule research. These advances are of importance for the investigation of biopolymers and cellular biochemical reactions, and are essential to the development of quantitative biology. Written by leading experts in the field, the articles cover a broad range of topics, including quantum photonics of organic dyes and inorganic nanoparticles and monitoring of single molecule (enzymatic) reactions.

Single Molecule Approaches to Biology 2010 Gordon Research Conference June 27 JULY 2 2010 Italy

Single Molecule Approaches to Biology  2010 Gordon Research Conference  June 27 JULY 2  2010  Italy Book
Author : Anonim
Publisher : Unknown
Release : 2010
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

The 2010 Gordon Conference on Single-Molecule Approaches to Biology focuses on cutting-edge research in single-molecule science. Tremendous technical developments have made it possible to detect, identify, track, and manipulate single biomolecules in an ambient environment or even in a live cell. Single-molecule approaches have changed the way many biological problems are addressed, and new knowledge derived from these approaches continues to emerge. The ability of single-molecule approaches to avoid ensemble averaging and to capture transient intermediates and heterogeneous behavior renders them particularly powerful in elucidating mechanisms of biomolecular machines: what they do, how they work individually, how they work together, and finally, how they work inside live cells. The burgeoning use of single-molecule methods to elucidate biological problems is a highly multidisciplinary pursuit, involving both force- and fluorescence-based methods, the most up-to-date advances in microscopy, innovative biological and chemical approaches, and nanotechnology tools. This conference seeks to bring together top experts in molecular and cell biology with innovators in the measurement and manipulation of single molecules, and will provide opportunities for junior scientists and graduate students to present their work in poster format and to exchange ideas with leaders in the field. A number of excellent poster presenters will be selected for short oral talks. Topics as diverse as single-molecule sequencing, DNA/RNA/protein interactions, folding machines, cellular biophysics, synthetic biology and bioengineering, force spectroscopy, new method developments, superresolution imaging in cells, and novel probes for single-molecule imaging will be on the program. Additionally, the collegial atmosphere of this Conference, with programmed discussion sessions as well as opportunities for informal gatherings in the afternoons and evenings in the beauty of the Il Ciocco site in Tuscany, provides an avenue for scientists from different disciplines to interact and brainstorm and promotes cross-disciplinary collaborations directed toward compelling biological problems.

Single Molecule Biophysics

Single Molecule Biophysics Book
Author : Tamiki Komatsuzaki,Masaru Kawakami,Satoshi Takahashi,Haw Yang,Robert J. Silbey
Publisher : Wiley
Release : 2011-11-16
ISBN : 9781118131404
Language : En, Es, Fr & De

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Book Description :

Discover the experimental and theoretical developments in optical single-molecule spectroscopy that are changing the ways we think about molecules and atoms The Advances in Chemical Physics series provides the chemical physics field with a forum for critical, authoritative evaluations of advances in every area of the discipline. This latest volume explores the advent of optical single-molecule spectroscopy, and how atomic force microscopy has empowered novel experiments on individual biomolecules, opening up new frontiers in molecular and cell biology and leading to new theoretical approaches and insights. Organized into two parts—one experimental, the other theoretical—this volume explores advances across the field of single-molecule biophysics, presenting new perspectives on the theoretical properties of atoms and molecules. Single-molecule experiments have provided fresh perspectives on questions such as how proteins fold to specific conformations from highly heterogeneous structures, how signal transductions take place on the molecular level, and how proteins behave in membranes and living cells.This volume is designed to further contribute to the rapid development of single-molecule biophysics research. Filled with cutting-edge research reported in a cohesive manner not found elsewhere in the literature, each volume of the Advances in Chemical Physics series serves as the perfect supplement to any advanced graduate class devoted to the study of chemical physics.

Single Molecule Enzymology

Single Molecule Enzymology Book
Author : Gregory I. Mashanov,Christopher Batters
Publisher : Humana Press
Release : 2011-08-04
ISBN : 9781617792601
Language : En, Es, Fr & De

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Book Description :

The last fifteen years have witnessed the birth and maturation of many original methods and the development of protocols specific to single molecule measurements and their analysis, including techniques involving optical imaging, electron microscopy, optical and magnetic trapping, and developments in atomic force microscopy. In Single Molecule Enzymology: Methods and Protocols, experts in the field provide procedures which enable the extraction of detailed information about enzyme work cycles, their static and kinetic properties, and information about their location and activity within cells. The detailed volume offers practical advice on many aspects of single molecule enzymology and includes strategic overviews of interconnected methods involved in sample preparation, single molecule measurements, and data analysis. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Single Molecule Enzymology: Methods and Protocols is intended for use within the diverse community of molecular biologists, biochemists, and biophysicists studying enzymes in detail and can be used by researchers planning their first single molecule study or to aid more experienced researchers in further developing their existing studies.

Advances in Single molecule Research for Biology Nanoscience

Advances in Single molecule Research for Biology   Nanoscience Book
Author : Anonim
Publisher : Unknown
Release : 2011
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Advances in Single molecule Research for Biology Nanoscience book written by , available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Single Molecule Tools Part A Fluorescence Based Approaches

Single Molecule Tools  Part A  Fluorescence Based Approaches Book
Author : Anonim
Publisher : Academic Press
Release : 2010-08-17
ISBN : 9780080959276
Language : En, Es, Fr & De

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Book Description :

Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. Single Molecule Tools, Part A: Fluorescence Based Approaches captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools. Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools Methods included are useful to both established researchers and newcomers to the field Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines

Single Molecule Biophysics

Single Molecule Biophysics Book
Author : Tamiki Komatsuzaki,Masaru Kawakami,Satoshi Takahashi,Haw Yang,Robert J. Silbey
Publisher : Wiley
Release : 2011-11-16
ISBN : 9781118131404
Language : En, Es, Fr & De

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Book Description :

Discover the experimental and theoretical developments in optical single-molecule spectroscopy that are changing the ways we think about molecules and atoms The Advances in Chemical Physics series provides the chemical physics field with a forum for critical, authoritative evaluations of advances in every area of the discipline. This latest volume explores the advent of optical single-molecule spectroscopy, and how atomic force microscopy has empowered novel experiments on individual biomolecules, opening up new frontiers in molecular and cell biology and leading to new theoretical approaches and insights. Organized into two parts—one experimental, the other theoretical—this volume explores advances across the field of single-molecule biophysics, presenting new perspectives on the theoretical properties of atoms and molecules. Single-molecule experiments have provided fresh perspectives on questions such as how proteins fold to specific conformations from highly heterogeneous structures, how signal transductions take place on the molecular level, and how proteins behave in membranes and living cells.This volume is designed to further contribute to the rapid development of single-molecule biophysics research. Filled with cutting-edge research reported in a cohesive manner not found elsewhere in the literature, each volume of the Advances in Chemical Physics series serves as the perfect supplement to any advanced graduate class devoted to the study of chemical physics.

Biology s Wires and Motors

Biology s Wires and Motors Book
Author : Ahmad Samir Khalil,Massachusetts Institute of Technology. Department of Mechanical Engineering
Publisher : Unknown
Release : 2009
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

(cont.) Single-molecule assays centered around the "zinc fingered-phages" are being developed to establish M13 as a standalone, single-molecule building block for achieving universal connectivity and for unlocking future studies on the nature of protein-DNA interactions. Kinesin is an ATPase that "walks" processively along biofilament tracks to perform vital cellular processes. Whereas M13 mechanics were studied in the context of classical rod or polymer bending, our focus within kinesin mechanics is the molecular aspects of motility. While significant progress has been made in elucidating the broad features of the kinesin mechanochemical cycle, details of the force generation mechanism remain a mystery. Com3 bined efforts in molecular biology, optical trapping, and molecular simulation were employed to put forth a novel mechanism for the motor's power stroke, namely that it is produced when the conserved N-terminal cover strand forms a [beta]-sheet with the neck linker to yield the cover-neck bundle. In agreement with simulation, single-molecule motility data revealed impairment of the force-generating capacity of cover strand mutants, as measured by a reduction in stall force from the wild-type. Motility data also suggest that targeting forcegenerating elements, such as the cover strand, is a plausible strategy for designing biological motors with tunable motile properties, bringing us one step closer to a complete blueprint of kinesin's parts and their collective functioning.