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Introduction to Single Cell Omics

Introduction to Single Cell Omics Book
Author : Xinghua Pan,Shixiu Wu,Sherman M. Weissman
Publisher : Frontiers Media SA
Release : 2019-09-19
ISBN : 2889459209
Language : En, Es, Fr & De

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Book Description :

Single-cell omics is a progressing frontier that stems from the sequencing of the human genome and the development of omics technologies, particularly genomics, transcriptomics, epigenomics and proteomics, but the sensitivity is now improved to single-cell level. The new generation of methodologies, especially the next generation sequencing (NGS) technology, plays a leading role in genomics related fields; however, the conventional techniques of omics require number of cells to be large, usually on the order of millions of cells, which is hardly accessible in some cases. More importantly, harnessing the power of omics technologies and applying those at the single-cell level are crucial since every cell is specific and unique, and almost every cell population in every systems, derived in either vivo or in vitro, is heterogeneous. Deciphering the heterogeneity of the cell population hence becomes critical for recognizing the mechanism and significance of the system. However, without an extensive examination of individual cells, a massive analysis of cell population would only give an average output of the cells, but neglect the differences among cells. Single-cell omics seeks to study a number of individual cells in parallel for their different dimensions of molecular profile on genome-wide scale, providing unprecedented resolution for the interpretation of both the structure and function of an organ, tissue or other system, as well as the interaction (and communication) and dynamics of single cells or subpopulations of cells and their lineages. Importantly single-cell omics enables the identification of a minor subpopulation of cells that may play a critical role in biological process over a dominant subpolulation such as a cancer and a developing organ. It provides an ultra-sensitive tool for us to clarify specific molecular mechanisms and pathways and reveal the nature of cell heterogeneity. Besides, it also empowers the clinical investigation of patients when facing a very low quantity of cell available for analysis, such as noninvasive cancer screening with circulating tumor cells (CTC), noninvasive prenatal diagnostics (NIPD) and preimplantation genetic test (PGT) for in vitro fertilization. Single-cell omics greatly promotes the understanding of life at a more fundamental level, bring vast applications in medicine. Accordingly, single-cell omics is also called as single-cell analysis or single-cell biology. Within only a couple of years, single-cell omics, especially transcriptomic sequencing (scRNA-seq), whole genome and exome sequencing (scWGS, scWES), has become robust and broadly accessible. Besides the existing technologies, recently, multiplexing barcode design and combinatorial indexing technology, in combination with microfluidic platform exampled by Drop-seq, or even being independent of microfluidic platform but using a regular PCR-plate, enable us a greater capacity of single cell analysis, switching from one single cell to thousands of single cells in a single test. The unique molecular identifiers (UMIs) allow the amplification bias among the original molecules to be corrected faithfully, resulting in a reliable quantitative measurement of omics in single cells. Of late, a variety of single-cell epigenomics analyses are becoming sophisticated, particularly single cell chromatin accessibility (scATAC-seq) and CpG methylation profiling (scBS-seq, scRRBS-seq). High resolution single molecular Fluorescence in situ hybridization (smFISH) and its revolutionary versions (ex. seqFISH, MERFISH, and so on), in addition to the spatial transcriptome sequencing, make the native relationship of the individual cells of a tissue to be in 3D or 4D format visually and quantitatively clarified. On the other hand, CRISPR/cas9 editing-based In vivo lineage tracing methods enable dynamic profile of a whole developmental process to be accurately displayed. Multi-omics analysis facilitates the study of multi-dimensional regulation and relationship of different elements of the central dogma in a single cell, as well as permitting a clear dissection of the complicated omics heterogeneity of a system. Last but not the least, the technology, biological noise, sequence dropout, and batch effect bring a huge challenge to the bioinformatics of single cell omics. While significant progress in the data analysis has been made since then, revolutionary theory and algorithm logics for single cell omics are expected. Indeed, single-cell analysis exert considerable impacts on the fields of biological studies, particularly cancers, neuron and neural system, stem cells, embryo development and immune system; other than that, it also tremendously motivates pharmaceutic RD, clinical diagnosis and monitoring, as well as precision medicine. This book hereby summarizes the recent developments and general considerations of single-cell analysis, with a detailed presentation on selected technologies and applications. Starting with the experimental design on single-cell omics, the book then emphasizes the consideration on heterogeneity of cancer and other systems. It also gives an introduction of the basic methods and key facts for bioinformatics analysis. Secondary, this book provides a summary of two types of popular technologies, the fundamental tools on single-cell isolation, and the developments of single cell multi-omics, followed by descriptions of FISH technologies, though other popular technologies are not covered here due to the fact that they are intensively described here and there recently. Finally, the book illustrates an elastomer-based integrated fluidic circuit that allows a connection between single cell functional studies combining stimulation, response, imaging and measurement, and corresponding single cell sequencing. This is a model system for single cell functional genomics. In addition, it reports a pipeline for single-cell proteomics with an analysis of the early development of Xenopus embryo, a single-cell qRT-PCR application that defined the subpopulations related to cell cycling, and a new method for synergistic assembly of single cell genome with sequencing of amplification product by phi29 DNA polymerase. Due to the tremendous progresses of single-cell omics in recent years, the topics covered here are incomplete, but each individual topic is excellently addressed, significantly interesting and beneficial to scientists working in or affiliated with this field.

Single Cell Omics

Single Cell Omics Book
Author : Debmalya Barh,Vasco Azevedo
Publisher : Academic Press
Release : 2019-06-06
ISBN : 0128149205
Language : En, Es, Fr & De

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Book Description :

Single-Cell Omics: Volume 1: Technological Advances and Applications provides the latest technological developments and applications of single-cell technologies in the field of biomedicine. In the current era of precision medicine, the single-cell omics technology is highly promising due to its potential in diagnosis, prognosis and therapeutics. Sections in the book cover single-cell omics research and applications, diverse technologies applied in the topic, such as pangenomics, metabolomics, and multi-omics of single cells, data analysis, and several applications of single-cell omics within the biomedical field, for example in cancer, metabolic and neuro diseases, immunology, pharmacogenomics, personalized medicine and reproductive health. This book is a valuable source for bioinformaticians, molecular diagnostic researchers, clinicians and members of the biomedical field who are interested in understanding more about single-cell omics and its potential for research and diagnosis. Covers not only the technological aspects, but also the diverse applications of single cell omics in the biomedical field Summarizes the latest progress in single cell omics and discusses potential future developments for research and diagnosis Written by experts across the world, bringing different points-of-view and case studies to give a comprehensive overview on the topic

Single Cell Omics

Single Cell Omics Book
Author : Debmalya Barh,Vasco Azevedo
Publisher : Academic Press
Release : 2019-07-30
ISBN : 0128175338
Language : En, Es, Fr & De

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Book Description :

Single-cell Omics, Volume 2: Advances in Applications provides the latest single-cell omics applications in the field of biomedicine. The advent of omics technologies have enabled us to identify the differences between cell types and subpopulations at the level of the genome, proteome, transcriptome, epigenome, and in several other fields of omics. The book is divided into two sections: the first is dedicated to biomedical applications, such as cell diagnostics, non-invasive prenatal testing (NIPT), circulating tumor cells, breast cancer, gliomas, nervous systems and autoimmune disorders, and more. The second focuses on cell omics in plants, discussing micro algal and single cell omics, and more. This book is a valuable source for bioinformaticians, molecular diagnostic researchers, clinicians and several members of biomedical field interested in understanding more about single-cell omics and its potential for research and diagnosis. Covers the diverse single cell omics applications in the biomedical field Summarizes the latest progress in single cell omics and discusses potential future developments for research and diagnosis Written by experts across the world, it brings different points-of-view and study cases to fully give a comprehensive overview of the topic

Single Cell Omics

Single Cell Omics Book
Author : BCC Research
Publisher : Unknown
Release : 2017-12
ISBN : 9781622966158
Language : En, Es, Fr & De

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Book Description :

Download Single Cell Omics book written by BCC Research, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Single cell Omics

Single cell Omics Book
Author : Debmalya Barh,Vasco Azevedo
Publisher : Academic Press
Release : 2019-08-15
ISBN : 9780128149195
Language : En, Es, Fr & De

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Book Description :

Single-cell Omics: Technological Advances and Applications provides the latest technological developments and the applications of single-cell technologies in the field of biomedicine. The advent of omics technologies have enable us to identify the differences between cell types and subpopulations at the level of the genome, proteome, transcriptome, epigenome, and in several other fields of omics. In the current era of precision medicine, the single-cell omics technology is highly promising due to its potential in diagnosis, prognosis, and therapeutics. The book is divided in five parts: the first one brings an overview of single-cell omics researches and applications; the second deals with the diverse technologies applied in the topic, such as pangenomics, metabolomics, and multi-omics of single cells; the third is dedicated to data analysis; the forth one discusses the several applications of single-cell omics within biomedical field, for example in cancer, metabolic and neuro diseases, immunology, pharmacogenomics, personalized medicine and reproductive health; and a final discussion on future perspectives. This book is a valuable source for bioinformaticians, molecular diagnostic researchers, clinicians and several members of biomedical field interested in understanding more about single-cell omics and its potential for research and diagnosis. Covers not only the technological aspects, but also the diverse applications of single cell omics in the biomedical field Summarizes the latest progress in single cell omics and discusses potential future developments for research and diagnosis Written by experts across the world, bringing different points-of-view and case studies to give a comprehensive overview on the topic

Novel Single cell Omics Assays of Corticogenesis

Novel Single cell Omics Assays of Corticogenesis Book
Author : Ryan M.. Mulquee
Publisher : Unknown
Release : 2020
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Novel Single cell Omics Assays of Corticogenesis book written by Ryan M.. Mulquee, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Single Cell Analysis

Single Cell Analysis Book
Author : Anonim
Publisher : Unknown
Release : 2010
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Cellular heterogeneity arising from stochastic expression of genes, proteins, and metabolites is a fundamental principle of cell biology, but single cell analysis has been beyond the capabilities of 'Omics' technologies. This is rapidly changing with the recent examples of single cell genomics, transcriptomics, proteomics, and metabolomics. The rate of change is expected to accelerate owing to emerging technologies that range from micro/nanofluidics to microfabricated interfaces for mass spectrometry to third- and fourth-generation automated DNA sequencers. As described in this review, single cell analysis is the new frontier in Omics, and single cell Omics has the potential to transform systems biology through new discoveries derived from cellular heterogeneity.

Single Cell Analysis

Single Cell Analysis Book
Author : Tuhin Subhra Santra,Fan-Gang Tseng
Publisher : MDPI
Release : 2021-06-02
ISBN : 3036506284
Language : En, Es, Fr & De

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Book Description :

Cells are the most fundamental building block of all living organisms. The investigation of any type of disease mechanism and its progression still remains challenging due to cellular heterogeneity characteristics and physiological state of cells in a given population. The bulk measurement of millions of cells together can provide some general information on cells, but it cannot evolve the cellular heterogeneity and molecular dynamics in a certain cell population. Compared to this bulk or the average measurement of a large number of cells together, single-cell analysis can provide detailed information on each cell, which could assist in developing an understanding of the specific biological context of cells, such as tumor progression or issues around stem cells. Single-cell omics can provide valuable information about functional mutation and a copy number of variations of cells. Information from single-cell investigations can help to produce a better understanding of intracellular interactions and environmental responses of cellular organelles, which can be beneficial for therapeutics development and diagnostics purposes. This Special Issue is inviting articles related to single-cell analysis and its advantages, limitations, and future prospects regarding health benefits.

Handbook of Single Cell Technologies

Handbook of Single Cell Technologies Book
Author : Tuhin Subhra Santra,Fan-Gang Tseng
Publisher : Springer
Release : 2021-10-29
ISBN : 9789811089527
Language : En, Es, Fr & De

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Book Description :

This book provides a brief overview of single-cell analysis using recent advanced technologies. The different sections cover different aspect of single cell analysis and applications with their advantages, limitations, and future challenges. The book has covered how different physical energies such as optical, electrical, and mechanical energy have been applied for single cell therapy and analysis. The recent advanced micro/nanofluidic devices have been employed for single-cell counting, manipulation, cultivation, separation, isolation, lysis, printing and patterning and host-viral interaction at single-cell level. Various chemical approaches for single-cell analysis have been discussed, such as liposome mediated materials transfer at single-cell and their analysis, discovery of antibody via single-cell, high-throughput screening of antigen-specific antibody-secreting cells, and biomolecular secretion analysis of individual cells. Moreover, different single-cell omics such as genomics, proteomics and transcriptomics have been discussed using microfluidic technologies as well as conventional approaches. The role of single cell analysis in system biology and biocatalysis have been discussed in detail. The book describes single-cell phenotyping of heterogeneous tissue, stimulation, and instant reaction quenching technology for biochemical kinetic analysis, large scale single-cell assay for the identification of biocatalysts and analytical techniques for single-cell studies in microbiology. The role of single-cell analysis in cancer, such as single-cell adhesion and cancer progression, single-cell technologies for cancer therapy, analytical technology for single cancer cell analysis, and biophysical markers for cancer cell analysis have been discussed. The flow cytometry based high throughput single-cell analysis have been well emphasized. Finally this book has covered single-cell electrophysiology, single-cell sensing and size measurement using mechanical and microwave resonators, molecular force spectroscopy for cell adhesion measurement, micro-tweezers and force microscopy techniques for single-cell mechanobiological analysis, mass spectrometry and acoustic tweezers for single-cell manipulation and analysis. This book is intended for academic and industrial researchers, undergraduate and graduate students in the fields of biomedical engineering, bio-micro/nanoengineering, and bio-micro/nano fabrication for single-cell analysis. It can be used for courses on bio-MEMS/bio-NEMS, biomicrofluidics, bio-micro/nanofabrications, micro/nanofluidics, biophysics, single cell analysis, bionanotechnology, drug delivery systems and biomedical microdevices. Collective contributions from respected experts, have brought diverse aspects of single-cell technologies in a single hand book. This will benefit researchers and practitioners in the biotechnology industry for different diseases analysis, therapeutics, diagnostics, drug discovery, drug screening etc. In addition to hard copies, the book will be available online and will often be updated by the authors.

The Individual Microbe Single Cell Analysis and Agent Based Modelling

The Individual Microbe  Single Cell Analysis and Agent Based Modelling Book
Author : Johan H. J. Leveau,Ferdi L. Hellweger,Jan-Ulrich Kreft,Clara Prats,Weiwen Zhang
Publisher : Frontiers Media SA
Release : 2019-02-19
ISBN : 2889457494
Language : En, Es, Fr & De

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Book Description :

Recent technological advances in single-cell microbiology, using flow cytometry, microfluidics, x-ray fluorescence microprobes, and single-cell -omics, allow for the observation of individuals within populations. Simultaneously, individual-based models (or more generally agent-based models) allow for individual microbes to be simulated. Bridging these techniques forms the foundation of individual-based ecology of microbes (µIBE). µIBE has elucidated genetic and phenotypic heterogeneity that has important consequences for a number of human interests, including antibiotic or biocide resistance, the productivity and stability of industrial fermentations, the efficacy of food preservatives, and the potential of pathogens to cause disease. Individual-based models can help us to understand how these sets of traits of individual microbes influence the above. This eBook compiles all publications from a recent Research Topic in Frontiers in Microbiology. It features recent research where individual observational and/or modelling techniques are applied to gain unique insights into the ecology of microorganisms. The Research Topic “The Individual Microbe: Single-Cell Analysis and Agent-Based Modelling” arose from the 2016 @ASM conference of the same name hosted by the American Society for Microbiology at its headquarters in Washington, D.C. We are grateful to ASM for funding and hosting this conference.

Single Cell Methods

Single Cell Methods Book
Author : Valentina Proserpio
Publisher : Unknown
Release : 2019
ISBN : 9781493992423
Language : En, Es, Fr & De

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Book Description :

This volume provides a comprehensive overview for investigating biology at the level of individual cells. Chapters are organized into eight parts detailing a single-cell lab, single cell DNA-seq, RNA-seq, single cell proteomic and epigenetic, single cell multi-omics, single cell screening, and single cell live imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Single Cell Methods: Sequencing and Proteomics aims to make each experiment easily reproducible in every lab.

Single Cell Sequencing and Systems Immunology

Single Cell Sequencing and Systems Immunology Book
Author : Xiangdong Wang
Publisher : Springer
Release : 2015-03-27
ISBN : 9401797536
Language : En, Es, Fr & De

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Book Description :

The volume focuses on the genomics, proteomics, metabolomics, and bioinformatics of a single cell, especially lymphocytes and on understanding the molecular mechanisms of systems immunology. Based on the author’s personal experience, it provides revealing insights into the potential applications, significance, workflow, comparison, future perspectives and challenges of single-cell sequencing for identifying and developing disease-specific biomarkers in order to understand the biological function, activation and dysfunction of single cells and lymphocytes and to explore their functional roles and responses to therapies. It also provides detailed information on individual subgroups of lymphocytes, including cell characters, function, surface markers, receptor function, intracellular signals and pathways, production of inflammatory mediators, nuclear receptors and factors, omics, sequencing, disease-specific biomarkers, bioinformatics, networks and dynamic networks, their role in disease and future prospects. Dr. Xiangdong Wang is a Professor of Medicine, Director of Shanghai Institute of Clinical Bioinformatics, Director of Fudan University Center for Clinical Bioinformatics, Director of the Biomedical Research Center of Zhongshan Hospital, Deputy Director of Shanghai Respiratory Research Institute, Shanghai, China.

The Applications of Single cell Genomic Analysis in Development and Disease

The Applications of Single cell Genomic Analysis in Development and Disease Book
Author : QIN AN
Publisher : Unknown
Release : 2020
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Single-cell genomics is the study of molecular modalities, or "-omes" from individual cells. Many protocols have been developed to profile genome, epigenome, transcriptome, and proteome from single cells. Among all these protocols, single-cell transcriptome profiling using single-cell RNA sequencing is the most popular and mature one. This technique has been demonstrated to be very powerful in dissecting cell types within a heterogeneous tissue, as well as revealing cell type specific responses to stimuli. It has also been used to reconstruct cell trajectory during complex biological progress, such as cell differentiation. More importantly, it can be used to reveal gene co-expression networks among cell types, and ultimately the molecular mechanism of gene regulation. In the first two projects of my thesis, I described how we use single-cell RNA sequencing to understand the molecular mechanism controlling trophoblasts proliferation and differentiation during human peri-implantation embryo development, as well as the mechanism of retinal progenitor cells commitment during early human retinogenesis. In the first project, we profiled human embryonic stem cell derived retinal organoids using single-cell RNA sequencing, to understand the molecular mechanism of early retinogenesis. The development of the mammalian retina is a complicated process involving generating distinct types of neurons from retinal progenitor cells (RPCs) in a spatiotemporal-specific manner. The progression of RPCs during retinogenesis includes RPC proliferation, cell fate commitment, and specific neuronal differentiation. In this study, by performing single-cell RNA-sequencing (scRNA-seq) on cells isolated from human embryonic stem cell (hESC)-derived 3D retinal organoids, we successfully deconstructed the temporal progression of RPCs during early human retinogenesis. We identified two distinct subtypes of RPCs with unique molecular profiles, namely multipotent RPCs and neurogenic RPCs. We found genes related to the Notch and Wnt signaling pathway, as well as chromatin remodeling, were dynamically regulated during RPC commitment. Interestingly, our analysis identified CCND1, a G1-phase cell cycle regulator, was co-expressed with ASCL1 in a cell-cycle independent manner. Temporally-controlled overexpression of CCND1 in retinal organoids demonstrated a role for CCND1 in promoting early retinal neurogenesis. Together, our results revealed critical pathways and novel genes in the early retinogenesis of humans. In the second project, we profiled transcriptome from individual trophoblast cells collected from human peri-implantation embryos, to reveal how these cells proliferate and differentiate to establish placenta. Multipotent trophoblasts undergo dynamic morphological movement and cellular differentiation after conceptus implantation to generate placenta. However, the mechanism controlling trophoblast development and differentiation during peri-implantation development in human remains elusive. In this study, we modeled human conceptus peri-implantation development from blastocyst to early post-implantation stages by using an in vitro coculture system and profiled the transcriptome of 476 individual trophoblast cells from these conceptuses. We revealed the genetic networks regulating peri-implantation trophoblast development. While determining when trophoblast differentiation happens, our bioinformatic analysis identified T-box transcription factor 3 (TBX3) as a key regulator for the differentiation of cytotrophoblast into syncytiotrophoblast. The function of TBX3 in trophoblast differentiation is then validated by a loss-of-function experiment. In conclusion, our results provided a valuable resource to study the regulation of trophoblasts development and differentiation during human peri-implantation development. In parallel with the development of single-cell RNA sequencing, many efforts have been put in profiling other molecular modalities from single cells, such as genome, epigenome, and proteome. By elaborately combining these protocols, we can profile more than one types of "omes" from individual cells simultaneously. These techniques, commonly termed as "single-cell multimodal profiling", can generate data that has certain advantages compared to single-cell "mono-omics" approaches. Specifically, since each molecular modality provides orthogonal information about cell identities and status, the joint clustering of single-cell multi-omics data can better resolve cell types within a heterogeneous cell population. Also, because more than one molecular modalities were profiled simultaneously from every single cell, we could have better inferences about the relationship between these omics. In the third project, we demonstrated how to use scMT-seq (simultaneous profiling of transcriptome and DNA methylome from a single cell), to investigate the gene regulatory role of DNA methylation in sensory neurons during peripheral nerve injury response and regeneration. DNA methylation is implicated in neuronal injury response and regeneration, but its role in regulating stable transcription changes in different types of dorsal root ganglion (DRG) neurons is unclear. In this study, we simultaneously profiled both the DNA methylome and mRNA transcriptome from single DRG neurons at different ages under either control or peripheral nerve injury condition. We found that age-related expression changes in Notch signaling genes and methylation changes at Notch receptor binding sites are associated with the age-dependent decline in peripheral nerve regeneration potential. Moreover, selective hypomethylation of AP-1 complex binding sites on regeneration-associated gene (RAG) promoters coincides with RAG transcriptional upregulation after injury. Consistent with the findings that different subtypes of DRG neurons exhibit distinct methylome changes upon injury responses, in a hybrid CAST/Ei; C57BL/6 genetic background, we further observed allele-specific gene regulation and methylation changes for many RAGs after injury. We suggest that the genetic background determines distinct allele-specific DNA methylomes, which contribute to age-dependent regulation and neuronal subtype-specific injury-responses in different mouse strains.

Statistical Methods for the Integrative Analysis of Single cell Multi omics Data

Statistical Methods for the Integrative Analysis of Single cell Multi omics Data Book
Author : Ricardo Argelaguet
Publisher : Unknown
Release : 2020
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Statistical Methods for the Integrative Analysis of Single cell Multi omics Data book written by Ricardo Argelaguet, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Novel Droplet Techniques for Integrative Single Cell Analyses

Novel Droplet Techniques for Integrative Single Cell Analyses Book
Author : Kara Kimberly Brower
Publisher : Unknown
Release : 2020
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

The last decade has yielded transformative new methods to analyze single cells, revealing new insights into cellular diversity, tissue organization, and organism development. Droplet microfluidics, in particular, has made it possible to characterize single cells by their genome, epigenome, and transcriptome at unprecedented scale. These techniques have given rise to the first cell atlases, catalogs of hundreds of thousands of cells classified by type across tissues, systems, and even whole organisms. However, despite these advances, researchers lack an unbiased, high-throughput means to link cellular phenotype to genotype within the same cell. This capability would allow single cell analyses to move beyond simply characterizing the heterogeneity within systems and toward a more mechanistic understanding of whole-cell regulation. Here I describe the development of a new technology, Droception, that marries the three most powerful single cell analysis techniques -- droplet microfluidics, flow cytometry, and next-generation sequencing -- to screen and sort cells by complex phenotypes with downstream sequencing. This technology has the potential to expand our mechanistic understanding of rare biological populations, identify novel cell types unresolved by current techniques, and unlock new avenues for single cell multi-omics.

Analyses of Genome Structures in Testate Amoeba Arcellinida Amoebozoa Uning Single cell omics and Flurescence Microscopy

Analyses of Genome Structures in Testate Amoeba  Arcellinida  Amoebozoa  Uning Single cell  omics and Flurescence Microscopy Book
Author : Ketty Munyenyembe
Publisher : Unknown
Release : 2020
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Analyses of Genome Structures in Testate Amoeba Arcellinida Amoebozoa Uning Single cell omics and Flurescence Microscopy book written by Ketty Munyenyembe, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Single cell Multi omics Analysis Reveals the Progression and the Specific T Cell Receptor of Ankylosing Spondylitis in Adalimumab Treatment

Single cell Multi omics Analysis Reveals the Progression and the Specific T Cell Receptor of Ankylosing Spondylitis in Adalimumab Treatment Book
Author : Anonim
Publisher : Unknown
Release : 2020
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Single cell Multi omics Analysis Reveals the Progression and the Specific T Cell Receptor of Ankylosing Spondylitis in Adalimumab Treatment book written by , available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Essentials of Single Cell Analysis

Essentials of Single Cell Analysis Book
Author : Fan-Gang Tseng,Tuhin Subhra Santra
Publisher : Springer
Release : 2016-01-21
ISBN : 3662491184
Language : En, Es, Fr & De

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Book Description :

This book provides an overview of single-cell isolation, separation, injection, lysis and dynamics analysis as well as a study of their heterogeneity using different miniaturized devices. As an important part of single-cell analysis, different techniques including electroporation, microinjection, optical trapping, optoporation, rapid electrokinetic patterning and optoelectronic tweezers are described in detail. It presents different fluidic systems (e.g. continuous micro/nano-fluidic devices, microfluidic cytometry) and their integration with sensor technology, optical and hydrodynamic stretchers etc., and demonstrates the applications of single-cell analysis in systems biology, proteomics, genomics, epigenomics, cancer transcriptomics, metabolomics, biomedicine and drug delivery systems. It also discusses the future challenges for single-cell analysis, including the advantages and limitations. This book is enjoyable reading material while at the same time providing essential information to scientists in academia and professionals in industry working on different aspects of single-cell analysis. Dr. Fan-Gang Tseng is a Distinguished Professor of Engineering and System Science at the National Tsing Hua University, Taiwan. Dr. Tuhin Subhra Santra is a Research Associate at the California Nano Systems Institute, University of California at Los Angeles, USA.

Single Cell Methods

Single Cell Methods Book
Author : Valentina Proserpio
Publisher : Unknown
Release : 2019
ISBN : 9781493992416
Language : En, Es, Fr & De

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Book Description :

This volume provides a comprehensive overview for investigating biology at the level of individual cells. Chapters are organized into eight parts detailing a single-cell lab, single cell DNA-seq, RNA-seq, single cell proteomic and epigenetic, single cell multi-omics, single cell screening, and single cell live imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Single Cell Methods: Sequencing and Proteomics aims to make each experiment easily reproducible in every lab.

Microfluidic Tools for Connecting Single Cell Optical and Gene Expression Phenotype

Microfluidic Tools for Connecting Single Cell Optical and Gene Expression Phenotype Book
Author : Jesse Qiuxu Zhang
Publisher : Unknown
Release : 2020
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

The single cell is the fundamental unit of biology. Understanding how the identity of individual cells in multicellular organisms contribute to their function remains a key question in Biology. Traditionally, most observations of cells were made through imaging-based techniques. Today, advances in Next Generation Sequencing have led to the widespread adoption of sequencing-based techniques for investigating the genotype and phenotype at single-cell resolution. Microfluidics, including droplet-based microfluidics, have been instrumental in the most successful commercial single-cell genomics platforms.Integrating sequencing and imaging techniques will provide additional information than either of the techniques alone. Both single-cell imaging and genomics techniques measure orthogonal targets, and when combined reveal additional insights into cellular function. However, when performing sequential single-cell assays, there currently exists a tradeoff between throughput and information content. This dissertation will describe progress made towards reducing that gap. I will describe novel microfluidic platforms and techniques and applications involving integrating single-cell sequencing and optical measurements at high throughput. The microfluidics tools that will be discussed in this Dissertation aim to be a platform for performing single-cell multi-parameter and multi-omics techniques that will help further our understanding of cellular identity and how genotype informs phenotype at the single-cell level.