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Reactive Species Detection in Biology

Reactive Species Detection in Biology Book
Author : Frederick A. Villamena
Publisher : Elsevier
Release : 2016-10-23
ISBN : 012420080X
Language : En, Es, Fr & De

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Book Description :

Reactive Species Detection in Biology: From Fluorescence to Electron Paramagnetic Resonance Spectroscopy discusses the reactive oxygen species that have been implicated in the pathogenesis of various diseases, presenting theories, chemistries, methodologies, and various applications for the detection of reactive species in biological systems, both in-vitro and in-vivo. Techniques covered include fluorescence, high performance chromatography, mass spectrometry, immunochemistry, and electron paramagnetic resonance spectroscopy. Probe design and development are also reviewed in order to advance new approaches in radical detection through synthesis, computations, or experimental applications. Reviews all current advances in radical detection Emphasizes chemical structures and reaction schemes fundamental to radical detection and identification Describes the uses, advantages, and disadvantages of various probe designs Examines new approaches to radical probe development

Reactive Oxygen Species in Chemistry Biology and Medicine

Reactive Oxygen Species in Chemistry  Biology  and Medicine Book
Author : A. Quintanilha
Publisher : Springer Science & Business Media
Release : 2013-06-29
ISBN : 1475704178
Language : En, Es, Fr & De

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Book Description :

A NATO Advanced Study Institute on "Oxygen Radicals in Biological Systems: Recent Progress and New Methods of Study" was held in Braga, Portugal between Sep tember 1 and September 14, 1985, in order to consider the basic chemistry and biochem istry of activated oxygen (both radical and non-radical species) and their effect in biolog ical systems. This book summarizes the main lectures given at this meeting. While there is no attempt to cover all the major topics in the expanding subject of oxidative mechanisms in biology, an effort has been made to provide overviews on some key aspects of this field. The authors have attempted to convey a clear picture of both what is known and what remains unclear in their respective subjects. Not only are some of the techniques used for detecting activated oxygen species described, but also their strengths and limi tations. The chemistry of many of these species is discussed and the biological and/or pathological implications are carefully reviewed. The medical and therapeutic aspects of some of the well established pathways of damage and protection are analyzed. It is our hope that the material included in this book might be useful for both researchers and teachers at the graduate level. The success of this meeting was to a large extent due to the tireless committment of Professor Alberto Amaral and Dr. Concei~a:o Rangel; without their outstanding efforts in dealing with all the aspects of the organization, this summer school would not have been possible.

Frontiers of Reactive Oxygen Species in Biology and Medicine

Frontiers of Reactive Oxygen Species in Biology and Medicine Book
Author : Kozi Asada,Toshikazu Yoshikawa
Publisher : Elsevier Science & Technology
Release : 1994
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Reflecting upon the progress since the discovery of superoxide dismutase, these papers cover such topics as: reactive species of oxygen and its detection in vivo and in vitro; antioxidative enzymes and compounds; and the molecular mechanisms for adaption to oxidative stress.

Peroxynitrite Detection in Biological Media

Peroxynitrite Detection in Biological Media Book
Author : Sabine Szunerits,Mekki Bayachou
Publisher : Royal Society of Chemistry
Release : 2015-10-21
ISBN : 1782620850
Language : En, Es, Fr & De

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Book Description :

Peroxynitrite is a powerful oxidiser which can damage a wide array of molecules within cells, including DNA and proteins, leading to apoptosis, inflammation or cancer. Peroxynitrite detection and quantification provides critical information in understanding its biological implications. Attempts to investigate the behavior of peroxynitrite in vivo and in vitro have been hampered by the difficulty in detecting this highly reactive oxygen species. This book presents the current state of the art in this research field with contributions from scientific leaders in the field. The chapters make clear the associated challenges and development for selective and sensitive detection of peroxynitrite. This book is a timely addition to the literature, as the first in the field, dedicated to detecting this molecule in vivo. It will be welcomed by the community particularly medicinal and analytical chemists, developers of sensors and probes and analytical equipment manufacturers.

Correlative Light and Electron Microscopy IV

Correlative Light and Electron Microscopy IV Book
Author : Anonim
Publisher : Academic Press
Release : 2021-03-18
ISBN : 0128220597
Language : En, Es, Fr & De

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Book Description :

Correlative Light and Electron Microscopy IV, Volume 162, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Besides the detailed description of protocols for CLEM technologies including time-resolution, Super resolution LM and Volume EM, new chapters cover Workflow (dis)-advantages/spiderweb, Serial section LM + EM, Platinum clusters as CLEM probes, Correlative Light Electron Microscopy with a transition metal complex as a single probe, SEM-TEM-SIMS, HPF-CLEM, A new workflow for high-throughput screening of mitotic mammalian cells for electron microscopy using classic histological dyes, and more. Contains contributions from experts in the field Covers topics using nano-SIMS and EDX for CLEM Presents recent advances and currently applied correlative approaches Gives detailed protocols, allowing for the application of workflows in one’s own laboratory setting Covers CLEM approaches in the context of specific applications Aims to stimulate the use of new combinations of imaging modalities

Oxidative Stress and Redox Regulation

Oxidative Stress and Redox Regulation Book
Author : Ursula Jakob,Dana Reichmann
Publisher : Springer Science & Business Media
Release : 2013-03-15
ISBN : 9400757875
Language : En, Es, Fr & De

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Book Description :

Many physiological conditions such as host defense or aging and pathological conditions such as neurodegenerative diseases, and diabetes are associated with the accumulation of high levels of reactive oxygen species and reactive nitrogen species. This generates a condition called oxidative stress. Low levels of reactive oxygen species, however, which are continuously produced during aerobic metabolism, function as important signaling molecules, setting the metabolic pace of cells and regulating processes ranging from gene expression to apoptosis. For this book we would like to recruit the experts in the field of redox chemistry, bioinformatics and proteomics, redox signaling and oxidative stress biology to discuss how organisms achieve the appropriate redox balance, the mechanisms that lead to oxidative stress conditions and the physiological consequences that contribute to aging and disease.

Studies on Fluorescent Probes for the Specific Detection of Reactive Oxygen Species and Reactive Nitrogen Species in Living Cells

Studies on Fluorescent Probes for the Specific Detection of Reactive Oxygen Species and Reactive Nitrogen Species in Living Cells Book
Author : Zhenning Sun,孫振宁
Publisher : Unknown
Release : 2017-01-27
ISBN : 9781361427873
Language : En, Es, Fr & De

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Book Description :

This dissertation, "Studies on Fluorescent Probes for the Specific Detection of Reactive Oxygen Species and Reactive Nitrogen Species in Living Cells" by Zhenning, Sun, 孫振宁, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Abstract of thesis entitled STUDIES ON FLUORESCENT PROBES FOR THE SPECIFIC DETECTION OF REACTIVE OXYGEN SPECIES AND REACTIVE NITROGEN SPECIES IN LIVING CELLS Submitted by SUN Zhen-Ning for the Degree of Doctor of Philosophy at The University of Hong Kong in September 2006 This project mainly focuses on the development of fluorescent probes for two biologically important oxidants - peroxynitrite and hypochlorite. On the basis of the similar chemical behavior between peroxynitrite - - (ONOO ) and peroxymonosulfate (HOOSO ), a reaction specific to peroxynitrite has been discovered (Scheme 1). On the basis of this specific reaction, several BODIPY-type fluorescent probes have been designed and synthesized according to the photoinduced electron transfer (PET) mechanism (Scheme 2). Among those probes, SZN-2 was found to be highly sensitive in specific detection of peroxynitrite 1 -- without interference from other competing oxidants, such as H O, O, NO, O, 2 2 2 2 and alkylperoxyl radical (ROO). Upon treatment with 7 equiv of peroxynitrite, probe SZN-2 showed up to 69-fold increase in fluorescence intensity. A linear correlation between emission intensity and concentration of peroxynitrite was observed. Moreover, the formation of peroxynitrite in living cells (J744.1 macrophages) can be visualized selectively by using probe SZN-2. This novel fluorescent probe should significantly facilitate studies of the biological roles of peroxynitrite in oxidative stress and many peroxynitrite-associated human diseases, such as rheumatoid arthritis, heart disease, atherosclerosis, and stroke. A fluorescent probe, SZN-3, has also been developed for the detection of hypochlorite on the basis of a specific reaction between methoxyphenol and hypochlorite (Scheme 3). This probe is highly sensitive and specific to hypochlorite. Upon treatment with 5 equiv of hypochlorite, probe SZN-3 showed up to 754-fold increase in fluorescence intensity. The reactivities of SZN-3 toward different reactive oxygen species (ROS) and reactive nitrogen species (RNS), including H O, 2 2 1 -- - - - - O, NO, O, OH, OCl, ONOO and alkylperoxyl radical (ROO ) were compared. 2 2 The results showed that fluorescence increased only upon reaction with OCl, demonstrating that probe SZN-3 has much higher selectivity towards hypochlorite than other ROS and RNS in an abiotic system. Moreover, using probe SZN-3, the formation of OCl has been successfully detected in an enzymatic system - (Myeloperoxidase/H O /Cl system) and in macrophages upon stimulation. This 2 2 new probe has provided an efficient tool for the evaluation of the roles of OCl in biological systems. Scheme 1 ONOO O OH CF CF RO 2.2 R = Me 2.1a R = H 3.23 Oxidant CF 3 No Reaction H O, OH, NO 2 2 RO 1 . O O 2, 2 R = Me 2.1a R = H 3.23 Scheme 2 O O CF CF 3 3 RO MeO NN NN B B Et NOC CONEt Et NOC CONEt F F F F 2 2 2 2 3.8 R = Me 2.14 (SZN-1) R = MOM 3.22 3.29 (SZN-2) R = H OH CF CF HO ONOO NN NN Et NOC CONEt Et NOC CONEt 2 2 F F 2 2 F F 3.29 (SZN-2) 2.15 non-fluorescent fluorescent Scheme 3 O O OMe OCl O O OH 4.1 4.2 OMe Oxidant No Reaction H O, OH, NO 2 2 1 . O O 2, 2 OH OMe

Reactive Oxygen Species in Biology and Human Health

Reactive Oxygen Species in Biology and Human Health Book
Author : Shamim I. Ahmad
Publisher : CRC Press
Release : 2017-12-19
ISBN : 1498735460
Language : En, Es, Fr & De

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Book Description :

Unlike other narrowly focused books, Reactive Oxygen Species in Biology and Human Health provides a comprehensive overview of ROS. It covers the current status of research and provides pointers to future research goals. Additionally, it authoritatively reviews the impact of reactive oxygen species with respect to various human diseases and discusses antioxidants and other compounds that counteract oxidative stress. Comprised of seven sections, the first section describes the introduction, detection, and production of ROS, emphasizing phenolic compounds and vitamin E for their abilities to act as antioxidants. This section also highlights the role of lipoprotein-associated oxidative stress. Section two addresses the importance of iron accumulation in the brain resulting in the development of a group of neurodegenerative disorders (NDs) and identifies several causative genes for neurodegeneration with brain iron accumulation (NBIA) associated with Parkinsonism-related disorders. The third section discusses a number of NDs, including amyotrophic lateral sclerosis (ALS), Alzheimer’s disease (AD), Huntington's disease (HD), epilepsy, and multiple sclerosis (MS). Section four addresses autoimmune diseases caused by ROS, including asthma, autoimmune liver diseases, rheumatoid arthritis, thyroid disease, primary biliary cirrhosis, and systemic lupus. Section five analyzes a number of different cancers, including lung cancer, breast cancer, and melanoma, along with possible treatment regimens. Section six discusses cardiovascular diseases (CVDs) induced by ROS, presents the ROS-associated complex biochemical processes inducing inflammation as an important cause of CVDs, and explains the roles carotenoids play in preventing CVDs. The final section addresses other human diseases induced by oxidative stress, including sickle cell disease, nonalcoholic steatohepatitis, retinopathy, fibromyalgia, chronic obstructive pulmonary disease, asthma, pulmonary hypertension, infertility, and aging of human skin.

Nanostructured Photocatalysts

Nanostructured Photocatalysts Book
Author : Van-Huy Nguyen,Dai-Viet N. Vo,Sonil Nanda
Publisher : Elsevier
Release : 2021-06-25
ISBN : 0128235969
Language : En, Es, Fr & De

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Book Description :

Nanostructured Photocatalysts: From Fundamental to Practical Applications offers a good opportunity for academic, industrial researchers and engineers to gain insights on the fundamental principles and updated knowledge on the engineering aspects and various practical applications of photocatalysis. This book comprehensively and systematically reviews photocatalytic fundamental aspects, ranging from reaction mechanism, kinetic modeling, nanocatalyst synthesis and design, essential material characterization using advanced techniques, and novel reactor design and scale-up. Future perspectives, techno-economical evaluation and lifecycle assessment of photocatalytic processes are also provided. Finally, a wide range of practical, important and emerging photocatalytic applications, namely wastewater treatment, air pollution remediation, renewable and green energy generation, and vital chemical production are thoroughly covered, making this book useful and beneficial for engineers, scientists, academic researchers, undergraduates and postgraduates. Provides a fundamental understanding of photocatalysis Covers all aspects of recent developments in photocatalytic processes and photocatalytic materials Focuses on advanced photocatalytic applications and future research advancements on energy, environment, biomedical, and other specialty fields Contains contributions from leading international experts in photocatalysis Presents a valuable reference for academic and industrial researchers, scientists and engineers

Turn on Fluorescent Probes for Detecting Nitric Oxide in Biology

Turn on Fluorescent Probes for Detecting Nitric Oxide in Biology Book
Author : Lindsey Elizabeth McQuade
Publisher : Unknown
Release : 2010
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Chapter 1. Investigating the Biological Roles of Nitric Oxide and Other Reactive Nitrogen Species Using Fluorescent Probes: This chapter presents an overview of recent progress in the field of reactive nitrogen species (RNS) sensing. Reactive nitrogen species, such as nitric oxide (NO) and its higher oxides, play important roles in cell signaling during many physiological and pathological events. Elucidation of the exact functions of these important biomolecules has been hampered by the inability to detect RNS reliably under biological conditions. A surge of research into RNS chemistry has resulted in the design of a new generation of fluorescent probes that are specific and sensitive for their respective RNS analytes. Progress in the field of nitric oxide, peroxynitrite, and nitroxyl sensing promises to advance our knowledge of important signaling events involving these species and should lead to a better understanding of oxidative biochemistry crucial to health and disease. Chapter 2. Mechanism of Nitric Oxide Reactivity and Fluorescence Enhancement of the NO-Specific Probe, CuFu1: The mechanism of the reaction of CuFu1 (FL1 = 2-{2-chloro-6-hydroxy-5-[(2- methylquinolin-8-ylamino)-methyl]-3-oxo-3H-xanthen-9-yl}benzoic acid) with NO to form FL1-NO in aqueous, buffered solutions was investigated. The reaction is first order in concentration of CuFL1, NO, and hydroxide ion. Rate saturation at high base concentrations is consistent with the fact that the protonation state of the secondary amine of the complex is crucial for reactivity. Based on this information, faster-reacting probes can be obtained by lowering the pKa of the secondary amine. The activation parameters for the reaction indicate that the mechanism is associative (ASI = -29 ± 3 cal/K-mol) and occurs with a modest thermal barrier (AHI = 9.7 ± 0.5 kcal/mol; Ea = 10.3 ± 0.5 kcal/mol). Variable pH EPR experiments indicate that as the secondary amine of CuFu1 is deprotonated, the electron density shifts yielding new spin-active species that has electron density localized on the deprotonated nitrogen atom. This result suggests that FL1-NO formation occurs when NO attacks the deprotonated secondary amine of the coordinated ligand, causing inner-sphere electron transfer to Cu(II) to form Cu(I) and subsequent FL 1-NO release from the metal. Chapter 3. Fluorescence-Based Nitric Oxide Sensing by Cu(II) Complexes that Can Be Trapped in Living Cells: A series of symmetrical, fluorescein-derived ligands appended with two derivatized 2- methyl-8-aminoquinolines were prepared and spectroscopically characterized. The ligands 2-{6-hydroxy-4,5-bis[(2-methylquinolin-8-ylamino)methyl]-3-oxo-3H-xanthen5 9-yl}benzoic acid (FL2), 2-{4,5-bis[(6-(2-ethoxy-2-oxoethoxy)-2-methylquinolin-8- ylamino)methyl]-6-hydroxy-3-oxo-3H-xanthen-9-yl}benzoic acid (FL2E), and 2,2'-{8,8'- [9-(2-Carboxyphenyl)-6-hydroxy-3-oxo-3H-xanthene-4,5-diyl]bis(methylene)bis(azanediyl) bis(2-methylquinolin-8,6-diyl)}bis(oxy)diacetic acid (FL2A) were designed to improve the dynamic range of previously described asymmetric systems, and the copper complex Cu2FL2E was constructed as a trappable NO probe that is hydrolyzed intracellularly to form Cu2FL2A. The ligands themselves are only weakly emissive and completely quenched in their Cu(II) complexes, which were generated in situ by combining each ligand with two equivalents of CuCl2 . The resulting complexes were investigated as fluorescent probes for nitric oxide. Upon introduction of excess NO under anaerobic conditions to buffered solutions of Cu2(FL2), Cu 2(FL2E), and Cu2(FL2A), the fluorescence increased by factors of 23 ± 3, 17 ± 2, and 27 ± 3, respectively. The corresponding rate constants for fluorescence turn-on were determined to be 0.006 ± 0.003 s-, 0.0058 ± 0.0009 s-4 and 0.010 ± 0.002 s4. The probes are highly specific for NO over other biologically relevant reactive oxygen and nitrogen species, as well as Zn(II), the metal ion for which structurally similar probes were designed to detect. Chapter 4. Visualization of Nitric Oxide Production in the Mouse Main Olfactory Bulb by a Cell-Trappable Copper(II) Fluorescent Probe: The visualization of NO production using fluorescence in tissue slices of the mouse main olfactory bulb is reported. This discovery was possible through the use of a novel, celltrappable probe for intracellular nitric oxide detection based on a symmetric scaffold with two NO-reactive sites. Ester moieties installed onto the fluorescent probe are cleaved by intracellular esterases to yield the corresponding negatively charged, cell-impermeable acids. The trappable ester probe Cu2(FL2E) and the membrane-impermeable acid derivative Cu2(FL2A) respond rapidly and selectively to NO in buffers that simulate biological conditions. Application of Cu2(FL2E) leads to detection of endogenously produced NO in cell cultures and olfactory bulb brain slices. Chapter 5. Dextran-Based Cell-Trappable Fluorescent Probes for Nitric Oxide Visualization in Living Cells: Two new cell-trappable fluorescent probes for nitric oxide are reported based on either incorporation of hydrolyzable esters or conjugation to aminodextran polymers. Both probes are highly selective for NO over other reactive oxygen and nitrogen species (RONS). The ability of these probes to image nitric oxide produced endogenously in Raw 264.7 cells by fluorescence is demonstrated. Chapter 6. A Cell-Trappable Fluorescent Probe for Detecting Biological Zinc: The synthesis and spectroscopic characterization of a new, cell-trappable fluorescent probe for Zn(II) is presented. This probe, 2-(4,5-bis((6-(2-ethoxy-2-oxoethoxy)quinolin- 8-yl)amino)methyl)-6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid (QZ2E) is poorly emissive in the off-state, but exhibits a dramatic, 120 ± 10-fold increase in fluorescence upon Zn(II) binding. This binding is selective for Zn(II) over other biologically relevant metal cations, toxic heavy metals, and most first-row transition metals, and is of appropriate affinity (Kdl = 150 ± 100 [tM, Kd2 = 3.5 ± 0.1 mM) to bind Zn(II) at physiological levels reversibly. In live cells, QZ2E localizes to the Gogli apparatus where it can detect Zn(II). It is cell membrane permeable until cleavage of its ester groups by intracellular esterases produces QZ2A, a negatively-charged acid that cannot cross the cell membrane. Appendix 1. Screening for bNOS Inhibitors in Bacillus anthracis: The incidence of anthrax infection by the Gram-positive bacterium Bacillus anthracis and the challenges of its treatment are presented. B. anthracis pathogenesis is critically dependent on NO production by the enzyme bacterial nitric oxide synthase (bNOS), a variant of the eukaryotic NOSes that does not contain a reductase domain required for catalysis. Using non-committed reductases in the cell, B. anthracis

Reactive Oxygen Species ROS Nanoparticles and Endoplasmic Reticulum ER Stress Induced Cell Death Mechanisms

Reactive Oxygen Species  ROS   Nanoparticles  and Endoplasmic Reticulum  ER  Stress Induced Cell Death Mechanisms Book
Author : Loutfy H. Madkour
Publisher : Academic Press
Release : 2020-06-27
ISBN : 0128224967
Language : En, Es, Fr & De

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Book Description :

Reactive Oxygen Species (ROS), Nanoparticles, and Endoplasmic Reticulum (ER) Stress-Induced Cell Death Mechanisms presents the role of ROS‒mediated pathways cellular signaling stress, endoplasmic reticulum (ER) stress, oxidative stress, oxidative damage, nanomaterials, and the mechanisms by which metalloids and nanoparticles induce their toxic effects. The book covers the ecotoxicology of environmental heavy metal ions and free radicals on macromolecules cells organisms, heavy metals‒induced cell responses, oxidative stress, the source of oxidants, and the roles of ROS, oxidative stress and oxidative damage mechanisms. It also examines the nanotoxicity, cytotoxicity and genotoxicity mechanisms of nanomaterials and the effects of nanoparticle interactions. Antioxidant defense therapy and strategies for treatment round out the book, making it an ideal resource for researchers and professional scientists in toxicology, environmental chemistry, environmental science, nanomaterials and the pharmaceutical sciences. Covers the ecotoxicology of environmental heavy metal ions and the interactions between specific heavy metals‒induced cell responses and oxidative stress Provides a better understanding of the mechanism of nanomaterial-induced toxicity as a first defense for hazard prevention Covers recent advances in new nanomedication technologies for the effects of NPs on oxidative stress, ROS and ER stress Discusses the effects of interactions between antioxidant defense therapy, ROS and strategies for treatment

Nitric Oxide Part C Biological and Antioxidant Activities

Nitric Oxide  Part C  Biological and Antioxidant Activities Book
Author : Lester Packer,Enrique Cadenas
Publisher : Gulf Professional Publishing
Release : 1999
ISBN : 9780121822026
Language : En, Es, Fr & De

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Book Description :

General Description of the Volume: Nitric Oxide, recently designated "Molecule of the Year," impinges on a wide range of fields in biological research, particularly in the areas of biomedicine and cell and organismal biology, as well as in fundamental chemistry. This volume will be a valuable resource for the experienced researcher as well as for those newly entering the field. This volume continues the coverage of new and important tools for the elucidation of Nitric Oxide action initiated in Volumes 268 and 269 of Methods in Enzymology. Techniques for researching the physiology and toxicity of nitric oxide in cellular and organismal systems are highlighted. General Description of the Series: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. Key Features * Biological Activity * NO Donors: Nitrosothiols and Nitroxyls * Peroxynitrite * Oxidant and Antioxidant Action

Biological Oxidants Generation and Injurious Consequences

Biological Oxidants  Generation and Injurious Consequences Book
Author : Charles G. Cochrane,Michael A. Gimbrone
Publisher : Elsevier
Release : 2013-10-22
ISBN : 1483191540
Language : En, Es, Fr & De

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Book Description :

Biological Oxidants: Generation and Injurious Consequences is a collection of papers that describes research work done concerning molecules of the intracellular and plasma membrane generating system in leukocytes; the intracellular interactions in oxidant-induced cellular injury; malignant transformation; and intracellular mechanism of oxidant formation. One paper reviews biological oxidants and the molecular basis of chronic granulomatous disease, particularly on compositions found within the plasma membrane. One such composition concerns the discovery of a flavocytochrome b as the central electron-transporting molecule in this system. Another paper examines the early injury of cells caused by external oxidants and the consequences of DNA damage. Other papers review the metabolism of vascular endothelial-derived reactive species, the calcium-modulated cellular effects of oxidants, as well as, the mechanism of inflammatory oxidant damage in tissues. This book is suitable for biochemists, microbiologists, and academicians whose works involve research of cell membranes.

Lipid Soluble Antioxidants

Lipid Soluble Antioxidants Book
Author : 3Island Press
Publisher : Unknown
Release : 1992-07-31
ISBN : 9783034874335
Language : En, Es, Fr & De

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Book Description :

Download Lipid Soluble Antioxidants book written by 3Island Press, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Electron Paramagnetic Resonance Volume 27

Electron Paramagnetic Resonance  Volume 27 Book
Author : Bela E. Bode,Damien M. Murphy,Victor Chechik
Publisher : Royal Society of Chemistry
Release : 2020-11-25
ISBN : 183916171X
Language : En, Es, Fr & De

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Book Description :

Electron paramagnetic resonance (EPR) applications remain highly significant in modern analytical science and this volume compiles critical coverage of developments in the recent literature. The topics covered in this volume describe contrasting types of EPR application, including rapid scan EPR, using the EPR toolkit to investigate the structural dynamics of membrane proteins and pulse dipolar EPR spectroscopy for investigating biomolecular binding events. An additional chapter reviewing the PARACAT collaboration from the EU has also been included. Providing a snapshot of the area by a handpicked group of researchers at the cutting-edge of the field, this book is a useful addition to any library supporting this research.

Spectroscopic Techniques Artificial Intelligence for Food and Beverage Analysis

Spectroscopic Techniques   Artificial Intelligence for Food and Beverage Analysis Book
Author : Ashutosh Kumar Shukla
Publisher : Springer Nature
Release : 2020-08-20
ISBN : 9811564957
Language : En, Es, Fr & De

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Book Description :

This informative book discusses the various spectroscopic techniques applied in the analysis of food and beverages. The respective chapters cover techniques such as Laser-Induced Breakdown Spectroscopy (LIBS), FTIR spectroscopy, Electron Spin Resonance (ESR) spectroscopy and Thermoluminescence. The book also presents artificial intelligence applications that can be used to enhance the spectral data analysis experience in food safety and quality analysis. Given its scope, the book will appeal to novice researchers and students in the area of food science. It offers an equally exciting read for food scientists and engineers working in the food industry.

Oxidative Stress Diagnostic Methods and Applications in Medical Science

Oxidative Stress  Diagnostic Methods and Applications in Medical Science Book
Author : Pawan Kumar Maurya,Pranjal Chandra
Publisher : Springer
Release : 2017-10-24
ISBN : 9811047111
Language : En, Es, Fr & De

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Book Description :

This book discusses the importance of oxidative stress, related biomarkers, and its diagnostic methods including bio-analytical and advanced detection systems. Oxidative stress is associated with diminished capacity of a biological system to overcome the overproduction of reactive oxygen species. Since oxidative stress has been suggested as a causative factor in many diseases, its prevention is vital, and there is an urgent need for oxidative stress monitoring using in vitro and in vivo models. Interdisciplinary approaches have lead to the development of various oxidative stress monitoring models for real-world clinical and biomedical applications. The development of such methods requires a broad understanding of biology, chemistry, molecular biology, immunology and microbiology. As such, the book is a valuable resource for students, researchers and clinicians interested in the fundamental as well as applied aspects of oxidative stress associated diseases.

Enzymes as Sensors

Enzymes as Sensors Book
Author : Anonim
Publisher : Academic Press
Release : 2017-03-21
ISBN : 0128092912
Language : En, Es, Fr & De

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Book Description :

Volume 589, the latest volume in the Methods in Enzymology series, focuses on enzymes as sensors. Contain contributions from leading authorities Informs and updates on all the latest developments in the field

Lipid Soluble Antioxidants Biochemistry and Clinical Applications

Lipid Soluble Antioxidants  Biochemistry and Clinical Applications Book
Author : ONG,PACKER
Publisher : Birkhäuser
Release : 2013-03-09
ISBN : 3034874324
Language : En, Es, Fr & De

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Book Description :

Antioxidants inhibit the formation and spread of free radicals which can be damaging in biological systems. Free radicals form in biological systems through metabolism, but it is also realized that exogenous environmental sources, such as radiation, food, and drugs, contribute significantly to the generation of free radicals in biological systems. Being reactive species, free radicals are short-lived and do not travel far from cellular targets. Their concentration in biological systems is very low and is difficult to detect directly by electron spin resonance spectroscopy (ESR). Indirect methods of reactions of radicals with specific biomolecules are also sufficiently sensitive to detect quantitatively their presence. Thus the response of antioxidant defenses which react with radical species, can serve as an indirect measure that free radicals have been formed. Redox-based antioxidants change their oxidation state and antioxidants become free radicals themselves. Often, however, the antioxidants give rise to more persistent free radicals, sometimes owing to delocalization of the lone electron around ring structures (in vitamin E, ubiquinones, and certain carotenes). Persistent free radicals react only rarely and the precursors often can be regenerated in biological systems. In recent years, it is becoming clearer from biochemical studies on how the major lipophilic antioxidants work. Particular attention has been given to vitamin E and quinones found in animal and plant membranes and in carotenoids, for the protection of membranes in lipoprotein systems. Flavonoids form another rich and varied source of natural antioxidants.

Handbook of Biochemistry and Molecular Biology Fourth Edition

Handbook of Biochemistry and Molecular Biology  Fourth Edition Book
Author : Roger L. Lundblad,Fiona Macdonald
Publisher : CRC Press
Release : 2010-05-21
ISBN : 1466515155
Language : En, Es, Fr & De

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Book Description :

Edited by renowned protein scientist and bestselling author Roger L. Lundblad, with the assistance of Fiona M. Macdonald of CRC Press, this fourth edition of the Handbook of Biochemistry and Molecular Biology represents a dramatic revision — the first in two decades — of one of biochemistry's most referenced works. This edition gathers a wealth of information not easily obtained, including information not found on the web. Offering a molecular perspective not available 20 years ago, it provides physical and chemical data on proteins, nucleic acids, lipids, and carbohydrates. Presented in an organized, concise, and simple-to-use format, this popular reference allows quick access to the most frequently used data. Covering a wide range of topics, from classical biochemistry to proteomics and genomics, it also details the properties of commonly used biochemicals, laboratory solvents, and reagents. Just a small sampling of the wealth of information found inside the handbook: Buffers and buffer solutions Heat capacities and combustion levels Reagents for the chemical modification of proteins Comprehensive classification system for lipids Biological characteristics of vitamins A huge variety of UV data Recommendations for nomenclature and tables in biochemical thermodynamics Guidelines for NMR measurements for determination of high and low pKa values Viscosity and density tables Chemical and physical properties of various commercial plastics Generic source-based nomenclature for polymers Therapeutic enzymes About the Editors: Roger L. Lundblad, Ph.D. Roger L. Lundblad is a native of San Francisco, California. He received his undergraduate education at Pacific Lutheran University and his PhD degree in biochemistry at the University of Washington. After postdoctoral work in the laboratories of Stanford Moore and William Stein at the Rockefeller University, he joined the faculty of the University of North Carolina at Chapel Hill. He joined the Hyland Division of Baxter Healthcare in 1990. Currently Dr. Lundblad is an independent consultant and writer in biotechnology in Chapel Hill, North Carolina. He is an adjunct Professor of Pathology at the University of North Carolina at Chapel Hill and Editor-in-Chief of the Internet Journal of Genomics and Proteomics. Fiona M. Macdonald, Ph.D., F.R.S.C. Fiona M. Macdonald received her BSc in chemistry from Durham University, UK. She obtained her PhD in inorganic biochemistry at Birkbeck College, University of London, studying under Peter Sadler. Having spent most of her career in scientific publishing, she is now at Taylor and Francis and is involved in developing chemical information products.