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Neuropeptide Technology

Neuropeptide Technology Book
Author : P. Michael Conn
Publisher : Academic Press
Release : 2013-10-22
ISBN : 1483259498
Language : En, Es, Fr & De

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Book Description :

Methods in Neurosciences, Volume 6: Neuropeptide Technology: Synthesis, Assay, Purification, and Processing describes procedures and tools of assay useful for the identification, purification, and quantification of neuropeptides and their receptors. This volume is divided into four sections— chemical synthesis and biosynthesis; measurement of neuropeptides; purification and characterization; and neuropeptide degrading and processing enzymes. In these sections, this book specifically discusses the synthesis of peptide substrates for protein kinase C; synthesis of glycosyl neuropeptides; and ultrastructural localization of peptides. The measurement of neurokinin B by radioimmunoassay; purification and characterization of neuroendocrine peptides from rat brain; and preparation of glia maturation factor ß are also elaborated. This text likewise covers the assays for arginine/lysine carboxypeptidases and enzymes that metabolize atrial natriuretic peptide. This publication is beneficial to neuroscientists and students researching on the synthesis, assay, purification, and processing of neuropeptides.

QUANTIFICATION OF NEUROPEPTIDE

QUANTIFICATION OF NEUROPEPTIDE Book
Author : 翁建霖,Kin-Lam Ken Yung
Publisher : Open Dissertation Press
Release : 2017-01-27
ISBN : 9781374735941
Language : En, Es, Fr & De

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Book Description :

This dissertation, "Quantification of Neuropeptides in the Central Nervous System of the Wobbler Mouse During the Progression of the Motor Neuron Disease: a Study by Radioimmunoassay and Immunocytochemistry" by 翁建霖, Kin-lam, Ken, Yung, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. DOI: 10.5353/th_b3121067 Subjects: Mice - Physiology Central nervous system Neuropeptides Motor neurons - Diseases Radioimmunoassay Immunocytochemistry - Technique

Neuropeptide Technology

Neuropeptide Technology Book
Author : P. Michael Conn
Publisher : Academic Press
Release : 2013-10-22
ISBN : 1483268136
Language : En, Es, Fr & De

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Book Description :

Methods in Neurosciences, Volume 5: Neuropeptide Technology: Gene Expression and Neuropeptide Receptors discusses procedures and tools of assay useful for the identification, purification, and quantification of neuropeptides and their receptors. This volume is divided into two sections—neuropeptide gene expression and neuropeptide receptors. In these sections, this book specifically discusses the assays for peptide products of somatostatin gene expression; molecular assays for rat thyrotropin-releasing hormone gene; and structure, expression, and chromosomal localization of human gene. The gastrin-releasing peptide or mammalian bombesin; semiquantitative analysis of cellular somatostatin mRNA Levels; and atrial natriuretic factor receptor assays are also elaborated. This text likewise covers the receptors for opioid peptides in brain and autoradiography of somatostatin receptors in rat cerebellum. This publication is beneficial to neuroscientists and students intending to acquire knowledge of gene expression and neuropeptide receptors.

Quantification of Neuropeptides in the Central Nervous System of the Wobbler Mouse During the Progression of the Motor Neuron Disease

Quantification of Neuropeptides in the Central Nervous System of the Wobbler Mouse During the Progression of the Motor Neuron Disease Book
Author : Anonim
Publisher : Unknown
Release : 1992
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Quantification of Neuropeptides in the Central Nervous System of the Wobbler Mouse During the Progression of the Motor Neuron Disease book written by , available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Determination of Structure activity Relationships for the Y2 Neuropeptide Y Receptor Subtype and Their Application Towards the Design of Small Highly Constrained Y2 Receptor Ligands

Determination of Structure activity Relationships for the Y2 Neuropeptide Y Receptor Subtype and Their Application Towards the Design of Small  Highly Constrained Y2 Receptor Ligands Book
Author : Robert Douglas Feinstein
Publisher : Unknown
Release : 1992
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Determination of Structure activity Relationships for the Y2 Neuropeptide Y Receptor Subtype and Their Application Towards the Design of Small Highly Constrained Y2 Receptor Ligands book written by Robert Douglas Feinstein, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Methods in Neurotransmitter and Neuropeptide Research

Methods in Neurotransmitter and Neuropeptide Research Book
Author : S.H. Parvez,M. Naoi,T. Nagatsu
Publisher : Elsevier
Release : 2013-10-22
ISBN : 1483290220
Language : En, Es, Fr & De

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Book Description :

This two-part book provides the most advanced biochemical and histochemical analytical methods on neurotransmitters, neuromodulators, and neuropeptides described by international specialists. The biochemical methods include liquid chromatography/electrochemistry, liquid chromatography/fluorescence and chemiluminescence detection, gas chromatography or liquid chromatography with mass spectrometry, and immunoassays. Morphological and physiological methods are also described, including immunohistochemistry, in situ hybridization, in vivo microdialysis, electrophysiological analysis, microwave irradiation, electrophysiological analysis, micro-anatomical dissection, peptide sequence analysis, quantitative autoradiographic analysis, and isolated CNS preparations. The neurotransmitters and neuropeptides include catecholamines, indoleamines, histamine, polyamines, and various neuropeptides, and their related enzymes. The book is the most comprehensive, advanced and versatile volume on the biochemical, morphological and physiological analysis of neurotransmitters and neuropeptides.

Advancing Mass Spectrometry Methods for Quantification and Characterization of Peptides and Proteins in Complex Biological Samples

Advancing Mass Spectrometry Methods for Quantification and Characterization of Peptides and Proteins in Complex Biological Samples Book
Author : Anonim
Publisher : Unknown
Release : 2014
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Protein quantification using liquid chromatography mass spectrometry (LC-MS) is a critical component in the discovery and verification of protein candidate biomarkers. Label-free and label-based quantitative methods are both commonly employed, and both have advantages and disadvantages. Label-based methods are capable of analyzing relative protein abundances from multiple samples in one LC-MS experiment, thus enabling high throughput analysis. Unfortunately, the high cost of commercial labels prevents many groups from including them in their proteomics workflows. Our group previously developed a cost-effective isobaric label set, DiLeu, through dimethylation of L-leucine isotopes. The DiLeu reagent performed comparably to commercial options, but multiple improvements and variations to this reagent were needed. For example, the multiplexing capacity of the original DiLeu reagent only permits four quantitative channels, necessitating development of a new label with greater throughput. A portion of this thesis is dedicated to the design, synthesis, and characterization of a new 8-plex DiLeu reagent. This dissertation also recounts the development of isotopic DiLeu (iDiLeu), a 5-plex mass difference variant of DiLeu that allows for researchers to quantify analyte peptide concentrations from a four-point calibration curve in one LC-MS run. One glaring omission in the development of noncommercial isobaric labels is that no protein candidate biomarkers have been discovered by them. Our work rectifies this oversight by identifying several potential biomarkers using custom-synthesized 4-plex DiLeu isobaric tags from the urine of men diagnosed with lower urinary tract symptoms (LUTS). Quantitative strategies presented in this dissertation are complemented by advances in our neuropeptidomics methodologies. In one project, we study the role of protein degradation in crustacean neuropeptidomics and apply a heat stabilization strategy to mitigate the number of protein fragments that interfere with neuropeptide identification. The final project investigates an alternative method to matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) in crab neuropeptide and lipid imaging. This technique, nanostructure initiator mass spectrometry (NIMS), was unable to ionize neuropeptides in C. borealis brain tissue despite its capability to ionize lipids, showing that sometimes traditional methods like MALDI MSI remain advantageous over more recent but less vetted techniques. The future outlook for our LUTS collaboration and quantitative tag projects is positive because of the new directions still being explored. Overall, the new quantitative techniques developed in this dissertation and the advances in neuropeptide identification will benefit research groups interested in cost-effective label-based quantification methods and neuropeptide characterization in LC-MS and MSI studies.

Mass Spectrometry and Hyphenated Techniques in Neuropeptide Research

Mass Spectrometry and Hyphenated Techniques in Neuropeptide Research Book
Author : Jerzy Silberring,Ralph Ekman
Publisher : John Wiley & Sons
Release : 2002-05-02
ISBN : 9780471354932
Language : En, Es, Fr & De

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Book Description :

The first authoritative guide to the application of this vital analytical technique Mass spectrometry is a powerful analytical technique that is used to identify unknown compounds, to quantify known materials, and to elucidate the structural and chemical properties of molecules. In analyzing the effects of experimental drugs on the brain, it is the sole technique for identifying the presence and structure of neuropeptides-substances that indicate the effect of the drug. Mass Spectrometry and Hyphenated Techniques in Neuropeptide Research specifically explains how to apply the technology to this process. Because the book is written by mass spectrometry users, as opposed to mass spectrometrists, the focus remains on practical applications of the technique. The authors demonstrate how mass spectrometry works, how to apply the technique to research, which types of instrumentation should be used for particular requirements, and how to plan experiments. Readers will learn why mass spectrometry provides more outcome features than other techniques in neuropeptide analysis, including simultaneous detection, identification of substances present in mixtures, and sequence information even when the residues are modified, blocked, or unusual. Among the chapters in this comprehensive text are: * Sequencing of Peptides by Nanospray Mass Spectrometry * Laser-Machined Microdevices for Mass Spectrometry * Electron Capture Dissociation of Peptides * Synthesis of Combinatorial Peptide Libraries * Analysis of Tissues That Reflect Nervous System Disease Doctoral students, researchers, and industry professionals in pharmacology, chemistry, biochemistry/biotechnology, and medicine will find Mass Spectrometry and Hyphenated Techniques in Neuropeptide Research to be an indispensable starting point for understanding peptides, their function, and identification.

Neuropeptide Protocols

Neuropeptide Protocols Book
Author : G. Brent Irvine,Carvell H. Williams
Publisher : Springer Science & Business Media
Release : 1997
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Leading scientists offer an unprecedented suite of master protocols for analyzing neuropeptide structure and function using optimized classic methods and the latest state-of-the-art techniques. This comprehensive collection includes valuable techniques for extracting and purifying neuropeptides from biological sources, for their sequence determination by Edman degradation and PCR, and for the solid-phase synthesis and purification of peptides and peptide analogs. Also covered are physical techniques for the characterization of synthetic or natural peptides, methods for immunocytochemical sample preparation and visualization at both light and electron levels, and techniques for neuropeptide-receptor interaction. Chapters on molecular modeling, radioisotopic labeling, production of antisera, the measurement of efflux from brain to blood, and the assay and inhibition of neuropeptidases complete this indispensable collection of readily reproducible protocols.

Neuropeptide Protocols Methods in Molecular Biology

Neuropeptide Protocols  Methods in Molecular Biology Book
Author : G. Brent Irvine,Carvell H. Wlliiams
Publisher : Unknown
Release : 1997
ISBN : 9781280836794
Language : En, Es, Fr & De

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Book Description :

Leading scientists offer an unprecedented suite of master protocols for analyzing neuropeptide structure and function using optimized classic methods and the latest state-of-the-art techniques. This comprehensive collection includes valuable techniques for extracting and purifying neuropeptides from biological sources, for their sequence determination by Edman degradation and PCR, and for the solid-phase synthesis and purification of peptides and peptide analogs. Also covered are physical techniques for the characterization of synthetic or natural peptides, methods for immunocytochemical sample preparation and visualization at both light and electron levels, and techniques for neuropeptide-receptor interaction. Chapters on molecular modeling, radioisotopic labeling, production of antisera, the measurement of efflux from brain to blood, and the assay and inhibition of neuropeptidases complete this indispensable collection of readily reproducible protocols.

Peptides Advances in Research and Application 2012 Edition

Peptides   Advances in Research and Application  2012 Edition Book
Author : Anonim
Publisher : ScholarlyEditions
Release : 2012-12-26
ISBN : 1464994994
Language : En, Es, Fr & De

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Book Description :

Peptides—Advances in Research and Application: 2012 Edition is a ScholarlyEditions™ eBook that delivers timely, authoritative, and comprehensive information about Peptides. The editors have built Peptides—Advances in Research and Application: 2012 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Peptides in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Peptides—Advances in Research and Application: 2012 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.

Analysis of Ovarian Ecdysteroidogenesis in the Yellow Fever Mosquito Aedes Aegypti linnaeus diptera Culicidae Ecdysone Quantification Neuropeptide Signaling and Cholesterol Mobilization

Analysis of Ovarian Ecdysteroidogenesis in the Yellow Fever Mosquito  Aedes Aegypti  linnaeus   diptera  Culicidae   Ecdysone Quantification  Neuropeptide Signaling  and Cholesterol Mobilization Book
Author : David Arthur McKinney
Publisher : Unknown
Release : 2017
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

The synthesis of ecdysone in insects is fundamental to their development and reproduction. Understanding how the production of this hormone occurs is vital to understanding these processes in insects, and the quantification of this hormone is a key component of this. With the dominant older method of routine ecdysteroid quantification becoming unavailable, a new, refined enzyme-linked immunoassay is developed in parallel with superior extraction methods for insect steroid hormones. Activation of ecdysteroid synthesis occurs in mosquito ovaries following a blood meal, mediated primarily by the binding of the neuropeptides ovary ecdysteroidogenic hormone (OEH) and insulin-like peptide 3 (ILP3) to their respective receptors in follicle cell membranes of the ovary. Both neuropeptides activate ecdysteroidogenesis through the protein kinase Akt, but the relationship of calcium to this activation is unknown. Given that calcium is an important component of ecdysteroid production in the prothoracic glands, it was predicted to have import for ovarian ecdysteroidogenesis. This work demonstrates a regulatory role of calcium in ecdysteroidogenesis. Another important aspect of ecdysone production by the ovaries is that of the cholesterol precursor for steroid synthesis. Since cholesterol is stored in esterified form, it is necessary to mobilize it for ecdysteroid production. The mobilization of cholesterol is relatively unexplored in insect hormone production, which is surprising, given the inability of insects to produce cholesterol de novo. The work here shows that the mosquito ovary, and specifically the follicle cells of the ovary, is proportionally richer in cholesterol than most of the other tissues in the mosquito. The mobilization of cholesterol was confirmed in that the same signals that activate ecdysteroidogenesis in the ovaries increase the amount of free cholesterol in isolated ovaries. This indicates that the source of cholesterol for such synthesis is contained within the ovaries. Additionally, a stringent requirement for follicle cell plasma membrane cholesterol was observed in cholesterol depletion and in vitro activation experiments. This suggests the role of increased free cholesterol in the follicle cell membranes is related to membrane receptor function.

Neuropeptide Receptors

Neuropeptide Receptors Book
Author : Ivone Gomes,Jonathan H. Wardman,Steven D. Jr. Stockton
Publisher : Unknown
Release : 2013-04-01
ISBN : 9781615044689
Language : En, Es, Fr & De

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Book Description :

Neuropeptides mediate their effects by binding and activating receptors that are responsible for converting these extracellular stimuli into intracellular responses. Most neuropeptides interact with G protein-coupled receptors that transduce the signal by activating heterotrimeric G proteins leading to alterations in second messenger systems to amplify the signal and elicit the intracellular response. In this review, we describe the general structure of G protein-coupled receptors including the information obtained from crystal structure determination that has given an insight into the activation mechanism of these receptors. In addition, we summarize the components of the signal transduction system (including G proteins, effectors and second messengers generally activated by the neuropeptide receptors). Using select examples of neuropeptide-receptor systems, we highlight the neuropeptides and corresponding receptors involved in modulation of pain and analgesia, body weight regulation, and hormonal regulation. Finally, we discuss the enzyme-linked tyrosine kinase receptors activated by growth factors and discuss the emerging concepts in targeting neuropeptide receptors for the identification of novel therapeutics targeting these systems. Table of Contents: Abbreviations / Overview of Neuropeptide Receptors / G Protein-Coupled Receptors: General Structure & Function / G Protein-Coupled Receptor Signaling / Neuropeptide Processing and Regulation / Neuropeptide Receptors / Perspectives / References

Mass Spectrometry in Cancer Research

Mass Spectrometry in Cancer Research Book
Author : John Roboz
Publisher : CRC Press
Release : 2002-03-25
ISBN : 1420042696
Language : En, Es, Fr & De

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Book Description :

Cancer research is becoming multidisciplinary. The complex structural and therapeutic problems require synergistic approaches employing an assortment of biochemical manipulations, chromatographic or electrophoretic separations, sequencing strategies, and ... more and more mass spectrometry. Mass Spectrometry in Cancer Research provides a broad examination of current strategies and techniques and their application to the study of: (i) occupational and environmental carcinogens; (ii) antineoplastic and chemopreventive agents; (iii) pertinent proteins, lipids, nucleic acids and glycoconjugates. Also included are a chapter on instrumentation and methodologies for biologists and physicians and a brief review of the relevant concepts of cancer biology and medicine for mass spectrometrists. This book is intended for: mass spectrometrists in research or those providing core services; researchers in biological, medical, pharmaceutical or environmental sciences; physicians in academic medicine; and academic/industrial research managers.

Cumulated Index Medicus

Cumulated Index Medicus Book
Author : Anonim
Publisher : Unknown
Release : 1996
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Cumulated Index Medicus book written by , available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Studies in Natural Products Chemistry

Studies in Natural Products Chemistry Book
Author : Atta-ur-Rahman
Publisher : Elsevier
Release : 2011-08-30
ISBN : 9780080458489
Language : En, Es, Fr & De

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Book Description :

Natural products present in the plant and animal kingdom offer a huge diversity of chemical structures which are the result of biosynthetic processes that have been modulated over the millennia through genetic effects. With the rapid developments in spectroscopic techniques and accompanying advances in high-throughput screening techniques, it has become possible to isolate, determine the structures and biological activity of natural products rapidly, thus opening up exciting new opportunities in the field of new drug development to the pharmaceutical industry. The present volume contains 22 articles written by leading experts in natural product chemistry on biologically active natural products. It includes research on a variety of different classes of natural products including sesquiterpenes, quassinoids, diterpenoids, lignans, oligostilbenes, phenylethanoids, phenylpropanoid glycosides, curcumin analogues, glycosphingolipids etc. Many of these have been found to be active in a number of different disease conditions. * Timely reviews written by international authorities in the field * Topics ranging from purely chemical to very biological * The 13th volume in the series to be devoted to bioactive natural products

Protein and Peptide Mass Spectrometry in Drug Discovery

Protein and Peptide Mass Spectrometry in Drug Discovery Book
Author : Michael L. Gross,Guodong Chen,Birendra Pramanik
Publisher : John Wiley & Sons
Release : 2011-09-26
ISBN : 1118116542
Language : En, Es, Fr & De

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Book Description :

The book that highlights mass spectrometry and its application in characterizing proteins and peptides in drug discovery An instrumental analytical method for quantifying the mass and characterization of various samples from small molecules to large proteins, mass spectrometry (MS) has become one of the most widely used techniques for studying proteins and peptides over the last decade. Bringing together the work of experts in academia and industry, Protein and Peptide Mass Spectrometry in Drug Discovery highlights current analytical approaches, industry practices, and modern strategies for the characterization of both peptides and proteins in drug discovery. Illustrating the critical role MS technology plays in characterizing target proteins and protein products, the methods used, ion mobility, and the use of microwave radiation to speed proteolysis, the book also covers important emerging applications for neuroproteomics and antigenic peptides. Placing an emphasis on the pharmaceutical industry, the book stresses practice and applications, presenting real-world examples covering the most recent advances in mass spectrometry, and providing an invaluable resource for pharmaceutical scientists in industry and academia, analytical and bioanalytical chemists, and researchers in protein science and proteomics.

Handbook of Biologically Active Peptides

Handbook of Biologically Active Peptides Book
Author : Abba Kastin
Publisher : Academic Press
Release : 2013-01-26
ISBN : 0123850967
Language : En, Es, Fr & De

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Book Description :

Handbook of Biologically Active Peptides, Second Edition, is the definitive, indispensable reference for peptide researchers, biochemists, cell and molecular biologists, neuroscientists, pharmacologists, and endocrinologists. Its chapters are designed to be a source for workers in the field and enable researchers working in a specific area to examine related areas outside their expertise. Peptides play a crucial role in many physiological processes, including actions as neurotransmitters, hormones, and antibiotics. Research has shown their importance in such fields as neuroscience, immunology, pharmacology, and cell biology. The second edition of Handbook of Biologically Active Peptides presents this tremendous body of knowledge in the field of biologically active peptides in one single reference. The section editors and contributors represent some of the most sophisticated and distinguished scientists working in basic sciences and clinical medicine. Presents all aspects of biologically active peptides in one resource Features more than 20 sections spanning plant, bacterial, fungal, venom, and invertebrate peptides to general peptides Includes immunological, inflammatory, cancer, vaccine, and neurotrophic peptides Discusses peptide precursors, mRNA distribution, processing, and receptors, not just pathophysiological implications

Ingle s Endodontics

Ingle s Endodontics Book
Author : Ilan Rotstein, DDS,John I. Ingle, DDS
Publisher : PMPH USA
Release : 2019-06-01
ISBN : 1607959356
Language : En, Es, Fr & De

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Book Description :

Ingle’s Endodontics, 7th edition, is the most recent revision of the text that has been known as the “Bible of Endodontics” for half a century. The new edition, published in two volumes, continues the tradition of including the expertise of international leaders in the field. Eighty-six authors contributed cutting-edge knowledge and updates on topics that have formed the core of this book for years. New chapters reflect the ways in which the field of endodontics has evolved over the 50 years since the pioneer John I. Ingle authored Endodontics. Ingle’s Endodontics will continue to be the standard against which all other endodontic texts will be measured. The 40 chapters are arranged in two volumes under three sections: The Science of Endodontics; The Practice of Endodontics: Diagnosis, Clinical Decision Making, Management, Prognosis; and Interdisciplinary Endodontics. With contributions from the world’s experts in all phases of the specialty, Ingle’s Endodontics, 7th edition promises to be an indispensable dentistry textbook, an essential part of every endodontist’s library.

Progress in Neuropeptide Research

Progress in Neuropeptide Research Book
Author : Elizabeth P. Farley
Publisher : Nova Publishers
Release : 2007
ISBN : 9781600216428
Language : En, Es, Fr & De

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Book Description :

The explosion of research activity in the field of neuropeptides has led to the identification of numerous naturally occurring endogenous peptides which act as neurotransmitters, neuromodulators, or trophic factors, to mediate nervous system functions. Increasing numbers of non-peptide ligands of neuropeptide receptors have been developed, which act as agonists or antagonists in peptidergic systems. The scope of this new and important book includes gene regulation of peptide expression, peptide receptor subtypes, transgenic and knockout mice with mutations in genes for neuropeptides and peptide receptors, neuroanatomy, physiology, behaviour, neurotrophic factors, preclinical drug evaluation, clinical studies, and clinical trials.