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Protocols In Biochemistry And Clinical Biochemistry

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Protocols in Biochemistry and Clinical Biochemistry

Protocols in Biochemistry and Clinical Biochemistry Book
Author : Shweta Pandey,Shyamal K. Goswami,Buddhi Prakash Jain
Publisher : Academic Press
Release : 2020-11-27
ISBN : 0128220082
Language : En, Es, Fr & De

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Book Description :

Protocols in Biochemistry and Clinical Biochemistry offers clear, applied instruction to fundamental biochemistry methods and protocols, from buffer preparation to nucleic acid purification, protein, lipid, carbohydrate, and enzyme testing, and clinical testing of vitamins, glucose and cholesterol levels, among other diagnostics. Each protocol is illustrated with step-by-step instructions, labeled diagrams, and color images, as well as a thorough overview of materials and equipment, precursor techniques, safety considerations and standards, analysis and statistics, alternative methods and troubleshooting. Includes full listings and discussion of materials and equipment, precursor techniques, safety considerations and standards, analysis and statistics, alternative methods and troubleshooting Features clear, step-by-step protocols and instructions with color diagrams and images

A Manual for Biochemistry Protocols

A Manual for Biochemistry Protocols Book
Author : Markus R. Wenk,Aaron Zefrin Fernandis
Publisher : World Scientific
Release : 2007
ISBN : 9812708316
Language : En, Es, Fr & De

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Book Description :

Biochemistry plays an important role in all areas of the biological and medical sciences. With most of the research or diagnosis involved in these areas being based on biochemically obtained observations, it is essential to have a profile of well standardized protocols. This manual is a basic guide for all students, researchers and experts in biochemistry, designed to help readers in directly starting off their experiments without prior knowledge of the protocol. The book dwells on the concepts used in designing the methodologies, thereby giving ample room for researchers to modify them according to their research requirements.

Basic Concepts in Clinical Biochemistry A Practical Guide

Basic Concepts in Clinical Biochemistry  A Practical Guide Book
Author : Vijay Kumar,Kiran Dip Gill
Publisher : Springer
Release : 2018-05-04
ISBN : 9789811081859
Language : En, Es, Fr & De

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Book Description :

This book is a practical guidebook in biochemistry, for medical as well as life sciences' students. The book covers reference values, sample collection procedure and detailed protocol to perform experiments. Each experiment starts with a brief introduction of the protocol, followed by specimen requirements and procedure. The procedures are presented in a very lucid manner and discuss details of calculations and clinical interpretations,The book is divided into 29 chapters, It offers references, general guidelines and abbreviations and provides principles and procedures of clinical biochemistry tests, along with their diagnostic importance.

Basic Concepts in Clinical Biochemistry A Practical Guide

Basic Concepts in Clinical Biochemistry  A Practical Guide Book
Author : Vijay Kumar,Kiran Dip Gill
Publisher : Springer
Release : 2019-02-11
ISBN : 9789811340864
Language : En, Es, Fr & De

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Book Description :

This book is a practical guidebook in biochemistry, for medical as well as life sciences' students. The book covers reference values, sample collection procedure and detailed protocol to perform experiments. Each experiment starts with a brief introduction of the protocol, followed by specimen requirements and procedure. The procedures are presented in a very lucid manner and discuss details of calculations and clinical interpretations,The book is divided into 29 chapters, It offers references, general guidelines and abbreviations and provides principles and procedures of clinical biochemistry tests, along with their diagnostic importance.

Advances in Clinical Chemistry

Advances in Clinical Chemistry Book
Author : Anonim
Publisher : Elsevier
Release : 2005-06-24
ISBN : 9780080458298
Language : En, Es, Fr & De

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Book Description :

Volume thirty-nine in the internationally acclaimed Advances in Clinical Chemistry, contains chapters submitted from leading experts from academia and clinical laboratory science. Authors are from a diverse field of clinical chemistry disciplines and diagnostics ranging from basic biochemical exploration to cutting edge microarray technology. In keeping with the tradition of the series, this volume emphasizes novel laboratory advances with application not only to both clinical laboratory diagnostics, but as well as practical basic science studies. This volume of Advances in Clinical Chemistry is an indispensable resource and practical guide for twenty-first century practitioners of clinical chemistry, molecular diagnostics, pathology, and clinical laboratory sciences in general. *Presents advances in assay methods such as immuno-PCR technology and proteomic assessment *Discusses the development and potential applications of novel biomarkers of chronic conditions (i.e,. Alzheimer's disease, cancer, cardiovascular disease and depression) *Addresses molecular and biochemical findings in the aging process

Clinical Metabolomics

Clinical Metabolomics Book
Author : Martin Giera
Publisher : Unknown
Release : 2018
ISBN : 9781493975921
Language : En, Es, Fr & De

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Book Description :

This detailed volume presents a comprehensive compendium of clinical metabolomics protocols covering LC-MS, GC-MS, CE-MS, and NMR-based clinical metabolomics as well as bioinformatics and study design considerations. The methodologies explored here form the core of several very promising initiatives evolving around personalized health care and precision medicine, which can be seen as complimentary to the field of clinical chemistry and aid the aforementioned field with novel disease markers and diagnostic patterns. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.uthoritative and practical, Clinical Metabolomics: Methods and Protocols aims to serve as the basis for successful communication between scientists from several fields, including chemists, biologist, bioinformaticians, and clinicians, ultimately leading to effective study design and completion.

Basic Concepts in Clinical Biochemistry

Basic Concepts in Clinical Biochemistry Book
Author : Vijay Kumar,Kiran Dip Gill
Publisher : Unknown
Release : 2018
ISBN : 9789811081873
Language : En, Es, Fr & De

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Book Description :

This book is a practical guidebook in biochemistry, for medical as well as life sciences' students. The book covers reference values, sample collection procedure and detailed protocol to perform experiments. Each experiment starts with a brief introduction of the protocol, followed by specimen requirements and procedure. The procedures are presented in a very lucid manner and discuss details of calculations and clinical interpretations, The book is divided into 29 chapters, It offers references, general guidelines and abbreviations and provides principles and procedures of clinical biochemistry tests, along with their diagnostic importance.

Advances in Clinical Chemistry

Advances in Clinical Chemistry Book
Author : Anonim
Publisher : Academic Press
Release : 2017-01-02
ISBN : 012812427X
Language : En, Es, Fr & De

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Book Description :

Advances in Clinical Chemistry, Volume 78, the latest installment in this internationally acclaimed series, contains chapters authored by world-renowned clinical laboratory scientists, physicians, and research scientists. The serial discusses the latest and most up-to-date technologies related to the field of clinical chemistry, and is the benchmark for novel analytical approaches in the clinical laboratory. Provides the most up-to-date technologies in clinical chemistry and clinical laboratory science Authored by world renowned clinical laboratory scientists, physicians, and research scientist Presents the international benchmark for novel analytical approaches in the clinical laboratory

Understanding Cancer

Understanding Cancer Book
Author : Buddhi Prakash Jain,Shweta Pandey
Publisher : Academic Press
Release : 2022-01-01
ISBN : 0323983960
Language : En, Es, Fr & De

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Book Description :

Understanding Cancer: From Basics to Therapeutics presents both basic concepts and research prospects of the field of cancer biology. It summarizes the fundamental aspects of cancer, and presents detailed description of molecular aspects as well as treatment and therapeutics for patients. The book is split into three parts: the first part deals with basics of the cancer, including aetiology and medical diagnosis; second part is about the molecular mechanisms associated with cancer, focusing on cell metabolism, epigenetics, and stem cells; and the third part is dedicated to therapeutics, discussing chemo and radiotherapies, gene, hormone and immunotherapies. It is a valuable resource for cancer researchers, oncologists, graduate students, and members of biomedical research who need to understand the fundamental topics related to cancer to apply in their research work or clinical setting. Presents fundamental aspects of cancer in a didactic way to make the content easily applicable by readers Illustrates the content through detailed images developed by the authors exclusively to the book to facilitate the comprehension of the topics Summarizes the content of each chapter with several tables and schematic diagrams for quick consult

High Throughput Metabolomics

High Throughput Metabolomics Book
Author : Angelo D'Alessandro
Publisher : Humana
Release : 2020-06-06
ISBN : 9781493992386
Language : En, Es, Fr & De

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Book Description :

This detailed volume focuses on recent technological, computational, and biostatistical advances in the field of high-throughput metabolomics. Chapters encompass methods, platforms, and analytical strategies for steady state measurements and metabolic flux analysis with stable isotope-labeled tracers, in biological matrices of clinical relevance and model organisms. Mass spectrometry-based or orthogonal methods are discussed, along with computational and statistical methods to address data sparsity in high-throughput metabolomics approaches. As a part of the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, High-Throughput Metabolomics: Methods and Protocols provides tools that can bring about the next generation of clinical biochemistry in a cost-effective, rigorous fashion, exponentially advancing our capacity to investigate nature while hastening the advent of personalized medicine.

Laboratory Protocols in Applied Life Sciences

Laboratory Protocols in Applied Life Sciences Book
Author : Prakash Singh Bisen
Publisher : CRC Press
Release : 2014-02-26
ISBN : 1466553146
Language : En, Es, Fr & De

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Book Description :

As applied life science progresses, becoming fully integrated into the biological, chemical, and engineering sciences, there is a growing need for expanding life sciences research techniques. Anticipating the demands of various life science disciplines, Laboratory Protocols in Applied Life Sciences explores this development. This book covers a wide spectrum of areas in the interdisciplinary fields of life sciences, pharmacy, medical and paramedical sciences, and biotechnology. It examines the principles, concepts, and every aspect of applicable techniques in these areas. Covering elementary concepts to advanced research techniques, the text analyzes data through experimentation and explains the theory behind each exercise. It presents each experiment with an introduction to the topic, concise objectives, and a list of necessary materials and reagents, and introduces step-by-step, readily feasible laboratory protocols. Focusing on the chemical characteristics of enzymes, metabolic processes, product and raw materials, and on the basic mechanisms and analytical techniques involved in life science technological transformations, this text provides information on the biological characteristics of living cells of different origin and the development of new life forms by genetic engineering techniques. It also examines product development using biological systems, including pharmaceutical, food, and beverage industries. Laboratory Protocols in Applied Life Sciences presents a nonmathematical account of the underlying principles of a variety of experimental techniques in disciplines, including: Biotechnology Analytical biochemistry Clinical biochemistry Biophysics Molecular biology Genetic engineering Bioprocess technology Industrial processes Animal Plant Microbial biology Computational biology Biosensors Each chapter is self-contained and written in a style that helps students progress from basic to advanced techniques, and eventually design and execute their own experiments in a given field of biology.

Clinical Applications of Capillary Electrophoresis

Clinical Applications of Capillary Electrophoresis Book
Author : Terry M. Phillips
Publisher : Unknown
Release : 2019
ISBN : 9781493992133
Language : En, Es, Fr & De

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Book Description :

Download Clinical Applications of Capillary Electrophoresis book written by Terry M. Phillips, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Nutritional Elements and Clinical Biochemistry

Nutritional Elements and Clinical Biochemistry Book
Author : Marge A. Brewster
Publisher : Springer Science & Business Media
Release : 2012-12-06
ISBN : 1461331684
Language : En, Es, Fr & De

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Book Description :

The important role that the nutritional status exerts in determining the course of life from birth to death in the human being and especially its impact in disease states is only par tially appreciated at this time. Nutritional deficiencies are usually considered to be major problems only in under-privileged or developing populations, except for those occurring in specific diseases. This attitude is incorrect as indicated by reports of Bestrian et al (1974, 1976) and Merritt and Suskin (1979) and others who found evidence of nutritional depletion in as much as 50% of the patients in varied groups of hospitalized patients in the United States. Other studies, some of which are included in this book, emphasized the existence of deficiencies of certain specific nutrients. Despite evidence of nutritional deficiencies occurring more frequently than previously appreciated, there is no well established protocol of laboratory studies that the clinical chemist or scientist should provide to help the physician detect lack of essential nutrients before extensive and possibly irreparable damage has occurred to the individual patient. Considerable research data are needed to determine the best biologic material (i.e., erythrocytes, leucocytes, plasma, serum, urine, cerebrospinal fluid, lymph) for analysis to determine accurately at an early stage metabolic deficiencies due to a specific nutritional element. Improved techniques for analysis of key metabolites and nutrients are available in the research labora tory and many of them can be adapted to the clinical laboratory.

Clinical Proteomics

Clinical Proteomics Book
Author : Antonia Vlahou
Publisher : Humana Press
Release : 2010-11-19
ISBN : 9781617377990
Language : En, Es, Fr & De

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Book Description :

In this book, a select group of researchers has contributed their state-of-the-art methodologies on protein profiling and identification of disease biomarkers in tissues, microdissected cells and body fluids. The book integrates biochemistry, pathology, analytical technology, bioinformatics, and proteome informatics. Experimental approaches are thoroughly detailed and explained through a step-by-step instructional format that ensures successful results.

Basic Techniques in Biochemistry Microbiology and Molecular Biology

Basic Techniques in Biochemistry  Microbiology and Molecular Biology Book
Author : Aakanchha Jain,Richa Jain,Sourabh Jain
Publisher : Humana
Release : 2020-01-24
ISBN : 9781493998609
Language : En, Es, Fr & De

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Book Description :

This book presents key methodologies, tools and databases for biochemistry, microbiology and molecular biology in simple and straightforward language. Covering all aspects related to experimental principles and procedures, the protocols included here are brief and clearly defined, and include essential precautions to be taken while conducting experiments. The book is divided into two major sections: one on constructing, working with, and standard operating procedures for laboratory instruments; and one on practical procedures used in molecular biology, microbiology and biochemical analysis experiments, which are described in full. Each chapter describes both the basic theory and relevant practical details for a given experiment, and helps readers recognize both the experiment’s potential and limitations. Intended as an intensive introduction to the various tools used in molecular biology, the book covers all basic methods and equipment, including cloning, PCR, spectrophotometers, ELISA readers, sonicators, etc. As such, it offers a valuable asset for final year undergraduate (especially project) students, graduate research students, research scientists and technicians who wish to understand and employ new techniques in the field of biotechnology.

Clinical Biochemistry

Clinical Biochemistry Book
Author : William J. Marshall,Stephen K. Bangert
Publisher : Elsevier Health Sciences
Release : 2008
ISBN : 0443101868
Language : En, Es, Fr & De

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Book Description :

Now fully revised and updated, Clinical Biochemistry, third edition is essential reading for specialty trainees, particularly those preparing for postgraduate examinations. It is also an invaluable current reference for all established practitioners, including both medical and scientist clinical biochemists. Building on the success of previous editions, this leading textbook primarily focuses on clinical aspects of the subject, giving detailed coverage of all conditions where clinical biochemistry is used in diagnosis and management - including nutritional disorders, diabetes, inherited metabolic disease, metabolic bone disease, renal calculi and dyslipidaemias. The acquisition and interpretation of clinical biochemical data are also discussed in detail. Expanded sections on haematology and immunology for clinical biochemists provide a thorough understanding of both laboratory and clinical aspects New chapters are included on important evolving areas such as the metabolic response to stress, forensic aspects of clinical biochemistry and data quality management An extended editorial team - including three expert new additions - ensures accuracy of information and relevance to current curricula and clinical practice A superb new accompanying electronic version provides an enhanced learning experience and rapid reference anytime, anywhere! Elsevier ExpertConsult.com Enhanced eBooks for medical professionals Compatible with PC, Mac®, most mobile devices and eReaders, browse, search, and interact with this title - online and offline. Redeem your PIN at expertconsult.com today! Straightforward navigation and search across all Elsevier titles Seamless, real-time integration between devices Adjustable text size and brightness Notes and highlights sharing with other users through social media Interactive content

Foundations and Applications of Medical Biochemistry in Clinical Practice

Foundations and Applications of Medical Biochemistry in Clinical Practice Book
Author : John Neustadt Nd,Steve Pieczenik MD Phd
Publisher : iUniverse
Release : 2009-06-13
ISBN : 9781440125348
Language : En, Es, Fr & De

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Book Description :

The culmination of more than ten years of research by the authors, this book describes for the first time ever the scientific basis and clinical applications of medical biochemistry, a fundamental paradigm shift in medicine. This paradigm shift is so revolutionary that it has been called the Neustadt-Pieczenik Paradigm, which is the fusion and clinical applications of biochemistry, thermodynamics, physiology, fractal enzymology, nutritional medicine and laboratory testing to identify and correct the underlying causes of many diseases that are considered genetic in nature (eg, Phenylketonuria) and those that are not considered genetic (eg, mature onset asthma, depression, fatigue). In this new medicine, doctors must reject the failed, purely symptomatic treatments they learned in medical school and focus on learning and treating the underlying biochemical causes of disease. From the first documented clinical observations of biochemical individuality in the early 1900s to the development of sophisticated biochemical tests, the authors provide a detailed and stunning analysis of a new medical model to help millions and cure our ailing healthcare system. They uniquely contrast the conventional medical approach with the functional biochemical approach through extensive case studies on depression, arthritis, migraine headaches, seizures, rashes and more. This book is a must-read for physicians, medical students, nutritionists, and anyone looking to take charge of their health.

Reviews and Protocols in DT40 Research

Reviews and Protocols in DT40 Research Book
Author : Jean-Marie Buerstedde,Shunichi Takeda
Publisher : Springer Science & Business Media
Release : 2006-10-21
ISBN : 9781402048968
Language : En, Es, Fr & De

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Book Description :

The vertebrate genome DT40 has proven to be a reliable and robust research subject, with fast doubling time, easy clonability and a relatively stable karyotype. This book provides an up to date overview of the different facets of research, and also intends to help newcomers get started and avoid looming pitfalls. The collection of protocols which have been provided by a number of laboratories will be particularly useful in this regard.

The Urinary Proteome

The Urinary Proteome Book
Author : Alex J. Rai
Publisher : Humana
Release : 2010-04-21
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Urine is one of the most easily accessible biological samples, and it provides a treasure trove of molecules essential for clinical diagnostics. In The Urinary Proteome: Methods and Protocols, expert researchers review briefly the classical urine tests that are performed in the clinical laboratory and then delve into the state of the art methods for proteomic analysis using urine specimens. Featuring the most recent advances in sample preparation, data analysis, and methods and applications, the book contains a multitude of detailed methods, including procedural details for the identification and characterization of urine biomarkers, which hold great potential for the diagnosis and treatment of many different disease conditions. Written in the highly successful Methods in Molecular BiologyTM series format, chapters present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Authoritative and systematic, The Urinary Proteome: Methods and Protocols is an ideal guide for scientific researchers, clinical laboratorians, clinical and translational scientists, and others interested in the vital study of proteomics and biomarker discovery.

Mass Spectrometry for the Clinical Laboratory

Mass Spectrometry for the Clinical Laboratory Book
Author : Hari Nair,William Clarke
Publisher : Academic Press
Release : 2016-11-02
ISBN : 0128009926
Language : En, Es, Fr & De

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Book Description :

Mass Spectrometry for the Clinical Laboratory is an accessible guide to mass spectrometry and the development, validation, and implementation of the most common assays seen in clinical labs. It provides readers with practical examples for assay development, and experimental design for validation to meet CLIA requirements, appropriate interference testing, measuring, validation of ion suppression/matrix effects, and quality control. These tools offer guidance on what type of instrumentation is optimal for each assay, what options are available, and the pros and cons of each. Readers will find a full set of tools that are either directly related to the assay they want to adopt or for an analogous assay they could use as an example. Written by expert users of the most common assays found in a clinical laboratory (clinical chemists, toxicologists, and clinical pathologists practicing mass spectrometry), the book lays out how experts in the field have chosen their mass spectrometers, purchased, installed, validated, and brought them on line for routine testing. The early chapters of the book covers what the practitioners have learned from years of experience, the challenges they have faced, and their recommendations on how to build and validate assays to avoid problems. These chapters also include recommendations for maintaining continuity of quality in testing. The later parts of the book focuses on specific types of assays (therapeutic drugs, Vitamin D, hormones, etc.). Each chapter in this section has been written by an expert practitioner of an assay that is currently running in his or her clinical lab. Provides readers with the keys to choosing, installing, and validating a mass spectrometry platform Offers tools to evaluate, validate, and troubleshoot the most common assays seen in clinical pathology labs Explains validation, ion suppression, interference testing, and quality control design to the detail that is required for implementation in the lab