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Proteomic Profiling And Analytical Chemistry

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Proteomic Profiling and Analytical Chemistry

Proteomic Profiling and Analytical Chemistry Book
Author : Pawel Ciborowski,Jerzy Silberring
Publisher : Newnes
Release : 2012-12-31
ISBN : 0444593918
Language : En, Es, Fr & De

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Book Description :

Proteomic Profiling and Analytical Chemistry helps scientists without a strong background in analytical chemistry to understand basic analytical principles and apply them to proteomics profiling. In most proteomic profiling experiments, liquid chromatography is used; this method is also used widely in analytical chemistry. This book bridges the gap between overly specialized courses and books in mass spectrometry, proteomics and analytical chemistry. It also helps researchers with an analytical chemistry background to break into the proteomics field. Proteomic Profiling and Analytical Chemistry focuses on practical applications for proteomic research helping readers to design better experiments and to more easily interpret, analyze and validate the resulting data. Experimental aspects such as sample preparation, protein extraction and precipitation, gel electrophoresis, microarrays, dynamics of fluorescent dyes, and more are all covered in detail. Covers the analytical consequences of protein and peptide modifications that may have a profound effect on how and what researchers actually measure Includes practical examples illustrating the importance of problems in quantitation and validation of biomarkers Helps in designing and executing proteomic experiments with sound analytics

Proteomic Profiling and Analytical Chemistry

Proteomic Profiling and Analytical Chemistry Book
Author : Pawel Ciborowski,Jerzy Silberring
Publisher : Elsevier
Release : 2016-03-02
ISBN : 0444636900
Language : En, Es, Fr & De

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Book Description :

Proteomic Profiling and Analytical Chemistry: The Crossroads, Second Edition helps scientists without a strong background in analytical chemistry to understand principles of the multistep proteomic experiment necessary for its successful completion. It also helps researchers who do have an analytical chemistry background to break into the proteomics field. Highlighting points of junction between proteomics and analytical chemistry, this resource links experimental design with analytical measurements, data analysis, and quality control. This targeted point of view will help both biologists and chemists to better understand all components of a complex proteomic study. The book provides detailed coverage of experimental aspects such as sample preparation, protein extraction and precipitation, gel electrophoresis, microarrays, dynamics of fluorescent dyes, and more. The key feature of this book is a direct link between multistep proteomic strategy and quality control routinely applied in analytical chemistry. This second edition features a new chapter on SWATH-MS, substantial updates to all chapters, including proteomic database search and analytical quantification, expanded discussion of post-hoc statistical tests, and additional content on validation in proteomics. Covers the analytical consequences of protein and peptide modifications that may have a profound effect on how and what researchers actually measure Includes practical examples illustrating the importance of problems in quantitation and validation of biomarkers Helps in designing and executing proteomic experiments with sound analytics

Mass Spectrometry Based Chemical Proteomics

Mass Spectrometry Based Chemical Proteomics Book
Author : W. Andy Tao,Ying Zhang
Publisher : John Wiley & Sons
Release : 2019-09-04
ISBN : 1118969553
Language : En, Es, Fr & De

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Book Description :

Emphasizing mass spectrometry, this book provides strategies and concepts for understanding and analyzing chemical proteomics, their functions, modifications, and interactions. Chapters cover technical advances and applications in drug discovery, from target identification to validation and potential treatments. Provides a basic overview of chemical proteomics, up-to-date concepts, analysis, and target validation Covers fundamentals and strategies, including proteome profiling and chemical probes Explains technical advances in the field and describes on shotgun proteomics, protein profiling, quantitative proteomics, peptide biosensors, and glycoprotein analysis Includes a wide variety of applications in drug discovery, from drug targets to the chemoproteomics analysis of natural products

Odorant Binding and Chemosensory Proteins

Odorant Binding and Chemosensory Proteins Book
Author : Anonim
Publisher : Academic Press
Release : 2020-09-02
ISBN : 012821158X
Language : En, Es, Fr & De

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Book Description :

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series

Advances in Biological Science Research

Advances in Biological Science Research Book
Author : Surya Nandan Meena,Milind Naik
Publisher : Academic Press
Release : 2019-05-17
ISBN : 0128174986
Language : En, Es, Fr & De

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Book Description :

Advances in Biological Science Research: A Practical Approach provides discussions on diverse research topics and methods in the biological sciences in a single platform. This book provides the latest technologies, advanced methods, and untapped research areas involved in diverse fields of biological science research such as bioinformatics, proteomics, microbiology, medicinal chemistry, and marine science. Each chapter is written by renowned researchers in their respective fields of biosciences and includes future advancements in life science research. Discusses various research topics and methods in the biological sciences in a single platform Comprises the latest updates in advanced research techniques, protocols, and methods in biological sciences Incorporates the fundamentals, advanced instruments, and applications of life science experiments Offers troubleshooting for many common problems faced while performing research experiments

Applications of Modern Mass Spectrometry Volume 1

Applications of Modern Mass Spectrometry  Volume 1 Book
Author : Atta-ur-Rahman, M. Iqbal Choudhary,Syed Ghulam Musharraf
Publisher : Bentham Science Publishers
Release : 2020-07-02
ISBN : 9811433801
Language : En, Es, Fr & De

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Book Description :

Applications of Modern Mass Spectrometry covers the latest advances in the use of mass spectrometry in scientific research. The series attempts to present readers information on the broad range of mass spectrometry techniques and configurations, data analysis and practical applications. Each volume contains contributions from eminent researchers who present their findings in an easy to read format. The multidisciplinary nature of the works presented in each volume of this book series make it a valuable reference on mass spectrometry to academic researchers and industrial R&D specialists in applied sciences, biochemistry, life sciences and allied fields. The first volume of the series presents 5 reviews: - Applications of mass spectrometry for the determination of the microbial crude protein synthesis in ruminants - Qualitative and quantitative LC-MS analysis in food proteins and peptides - Chemometrics as a powerful and complementary tool for mass spectrometry applications in life sciences - Recent developments of allied techniques of qualitative analysis of heavy metal ions in aqueous solutions with special reference to modern mass spectrometry - New techniques and methods in explosive analysis.

Applications in Forensic Proteomics

Applications in Forensic Proteomics Book
Author : Eric D. Merkley
Publisher : Unknown
Release : 2019
ISBN : 9780841236431
Language : En, Es, Fr & De

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Book Description :

Download Applications in Forensic Proteomics book written by Eric D. Merkley, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Essentials of Pharmaceutical Analysis

Essentials of Pharmaceutical Analysis Book
Author : Muhammad Sajid Hamid Akash,Kanwal Rehman
Publisher : Springer Nature
Release : 2019-12-17
ISBN : 9811515476
Language : En, Es, Fr & De

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Book Description :

Recent advances in the pharmaceutical sciences and biotechnology have facilitated the production, design, formulation and use of various types of pharmaceuticals and biopharmaceuticals. This book provides detailed information on the background, basic principles, and components of techniques used for the analysis of pharmaceuticals and biopharmaceuticals. Focusing on those analytical techniques that are most frequently used for pharmaceuticals, it classifies them into three major sections and 19 chapters, each of which discusses a respective technique in detail. Chiefly intended for graduate students in the pharmaceutical sciences, the book will familiarize them with the components, working principles and practical applications of these indispensable analytical techniques.

Issues in Industrial Applied and Environmental Chemistry 2011 Edition

Issues in Industrial  Applied  and Environmental Chemistry  2011 Edition Book
Author : Anonim
Publisher : ScholarlyEditions
Release : 2012-01-09
ISBN : 1464963770
Language : En, Es, Fr & De

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Book Description :

Issues in Industrial, Applied, and Environmental Chemistry: 2011 Edition is a ScholarlyEditions™ eBook that delivers timely, authoritative, and comprehensive information about Industrial, Applied, and Environmental Chemistry. The editors have built Issues in Industrial, Applied, and Environmental Chemistry: 2011 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Industrial, Applied, and Environmental Chemistry in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Issues in Industrial, Applied, and Environmental Chemistry: 2011 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.

Mass Spectrometry

Mass Spectrometry Book
Author : Marek Smoluch,Giuseppe Grasso,Piotr Suder,Jerzy Silberring
Publisher : John Wiley & Sons
Release : 2019-07-11
ISBN : 1119377307
Language : En, Es, Fr & De

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Book Description :

Provides a comprehensive description of mass spectrometry basics, applications, and perspectives Mass spectrometry is a modern analytical technique, allowing for fast and ultrasensitive detection and identification of chemical species. It can serve for analysis of narcotics, counterfeit medicines, components of explosives, but also in clinical chemistry, forensic research and anti-doping analysis, for identification of clinically relevant molecules as biomarkers of various diseases. This book describes everything readers need to know about mass spectrometry—from the instrumentation to the theory and applications. It looks at all aspects of mass spectrometry, including inorganic, organic, forensic, and biological MS (paying special attention to various methodologies and data interpretation). It also contains a list of key terms for easier and faster understanding of the material by newcomers to the subject and test questions to assist lecturers. Knowing how crucial it is for young researchers to fully understand both the power of mass spectrometry and the importance of other complementary methodologies, Mass Spectrometry: An Applied Approach teaches that it should be used in conjunction with other techniques such as NMR, pharmacological tests, structural identification, molecular biology, in order to reveal the true function(s) of the identified molecule. Provides a description of mass spectrometry basics, applications and perspectives of the technique Oriented to a broad audience with limited or basic knowledge in mass spectrometry instrumentation, theory, and its applications in order to enhance their competence in this field Covers all aspects of mass spectrometry, including inorganic, organic, forensic, and biological MS with special attention to application of various methodologies and data interpretation Includes a list of key terms, and test questions, for easier and faster understanding of the material Mass Spectrometry: An Applied Approach is highly recommended for advanced students, young scientists, and anyone involved in a field that utilizes the technique.

Identifying Microbes by Mass Spectrometry Proteomics

Identifying Microbes by Mass Spectrometry Proteomics Book
Author : Charles H. Wick
Publisher : CRC Press
Release : 2013-09-10
ISBN : 146650496X
Language : En, Es, Fr & De

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Book Description :

All microbes, including bacteria, viruses, and fungi, can be classified and identified by matching a few peptides known to be unique to each organism. Identifying Microbes by Mass Spectrometry Proteomics describes ways to identify microorganisms using powerful new techniques combining hardware and software and yielding highly accurate methods for detection, identification, and classification of microbes. This straightforward technology can be used to detect unknown and unsequenced microorganisms as well as microbes in complex environmental samples. This book reviews various mass analyzers used for detection and describes ionization methods frequently used for analysis of microbial constituents, a necessary step in the preparation of mass spectrometry (MS) samples. The text also discusses diverse processing methods, which are used to analyze MS files for matching mass spectral profiles, and examines protein and nucleic acid sequence-based methods capable of classification and identification of microbial agents. The book also covers sample collection methods and specific sample preparation techniques. The text addresses using computer software and bioinformatics approaches for data mining to discriminate microbes using mass spectrometry proteomics (MSP). It also discusses historical pattern recognition-based methods and other approaches such as analysis of pyrolysis products, chemical ionization (CI) of fatty acid methyl esters, and MALDI-MS. The text contains examples of the application of the MSP technique for microbe detection and includes a survey of suitable and commercially available MS-based platforms. Successful applications include the identification of unknown microbes in honey bees associated with colony collapse disorder and the analysis of virus strains from the 2009 influenza pandemic. The final chapter outlines future trends in these groundbreaking uses of MS techniques, which are fast, not limited by sample type, and show potential in answering complex environmental questions.

Principles and Applications of Soil Microbiology

Principles and Applications of Soil Microbiology Book
Author : Terry J. Gentry,Jeffry J. Fuhrmann,David A. Zuberer
Publisher : Elsevier
Release : 2021-06-06
ISBN : 0323851401
Language : En, Es, Fr & De

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Book Description :

Written by leading experts in their respective fields, Principles and Applications of Soil Microbiology 3e, provides a comprehensive, balanced introduction to soil microbiology, and captures the rapid advances in the field such as recent discoveries regarding habitats and organisms, microbially mediated transformations, and applied environmental topics. Carefully edited for ease of reading, it aids users by providing an excellent multi-authored reference, the type of book that is continually used in the field. Background information is provided in the first part of the book for ease of comprehension. The following chapters then describe such fundamental topics as soil environment and microbial processes, microbial groups and their interactions, and thoroughly addresses critical nutrient cycles and important environmental and agricultural applications. An excellent textbook and desk reference, Principles and Applications of Soil Microbiology, 3e, provides readers with broad, foundational coverage of the vast array of microorganisms that live in soil and the major biogeochemical processes they control. Soil scientists, environmental scientists, and others, including soil health and conservation specialists, will find this material invaluable for understanding the amazingly diverse world of soil microbiology, managing agricultural and environmental systems, and formulating environmental policy. Includes discussion of major microbial methods, embedded within topical chapters Includes information boxes and case studies throughout the text to illustrate major concepts and connect fundamental knowledge with potential applications Study questions at the end of each chapter allow readers to evaluate their understanding of the materials

Analytical Chemistry

Analytical Chemistry Book
Author : Harold H. Trimm
Publisher : CRC Press
Release : 2011-04-15
ISBN : 1466559764
Language : En, Es, Fr & De

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Book Description :

This title includes a number of Open Access chapters. This collection presents a broad selection of recent research on analytical chemistry, including methods of determination and analysis as applied to plants, pharmaceuticals, foods, proteins, and more. Analytical chemistry is the study of what chemicals are present and in what amount in natural and artificial materials. Because these understandings are fundamental in just about every chemical inquiry, analytical chemistry is used to obtain information, ensure safety, and solve problems in many different chemical areas, and is essential in both theoretical and applied chemistry. Analytical chemistry is driven by new and improved instrumentation.

Technologies to Enable Autonomous Detection for BioWatch

Technologies to Enable Autonomous Detection for BioWatch Book
Author : National Research Council,Institute of Medicine,Board on Life Sciences,Board on Health Sciences Policy
Publisher : National Academies Press
Release : 2013-12-31
ISBN : 0309292549
Language : En, Es, Fr & De

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Book Description :

The BioWatch program, funded and overseen by the Department of Homeland Security (DHS), has three main elements--sampling, analysis, and response--each coordinated by different agencies. The Environmental Protection Agency maintains the sampling component, the sensors that collect airborne particles. The Centers for Disease Control and Prevention coordinates analysis and laboratory testing of the samples, though testing is actually carried out in state and local public health laboratories. Local jurisdictions are responsible for the public health response to positive findings. The Federal Bureau of Investigation is designated as the lead agency for the law enforcement response if a bioterrorism event is detected. In 2003 DHS deployed the first generation of BioWatch air samplers. The current version of this technology, referred to as Generation 2.0, requires daily manual collection and testing of air filters from each monitor. DHS has also considered newer automated technologies (Generation 2.5 and Generation 3.0) which have the potential to produce results more quickly, at a lower cost, and for a greater number of threat agents. Technologies to Enable Autonomous Detection for BioWatch is the summary of a workshop hosted jointly by the Institute of Medicine and the National Research Council in June 2013 to explore alternative cost-effective systems that would meet the requirements for a BioWatch Generation 3.0 autonomous detection system, or autonomous detector, for aerosolized agents . The workshop discussions and presentations focused on examination of the use of four classes of technologies--nucleic acid signatures, protein signatures, genomic sequencing, and mass spectrometry--that could reach Technology Readiness Level (TRL) 6-plus in which the technology has been validated and is ready to be tested in a relevant environment over three different tiers of temporal timeframes: those technologies that could be TRL 6-plus ready as part of an integrated system by 2016, those that are likely to be ready in the period 2016 to 2020, and those are not likely to be ready until after 2020. Technologies to Enable Autonomous Detection for BioWatch discusses the history of the BioWatch program, the role of public health officials and laboratorians in the interpretation of BioWatch data and the information that is needed from a system for effective decision making, and the current state of the art of four families of technology for the BioWatch program. This report explores how the technologies discussed might be strategically combined or deployed to optimize their contributions to an effective environmental detection capability.

Proteomics in Biology

Proteomics in Biology Book
Author : Anonim
Publisher : Academic Press
Release : 2017-01-19
ISBN : 0128105348
Language : En, Es, Fr & De

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Book Description :

Proteomics in Biology Part A, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field, and a focus on proteomics for this updated volume. Continues the legacy of this premier serial with quality chapters that focus on proteomics Contains contributions from leading authorities

Proteomics in Biology

Proteomics in Biology Book
Author : Anonim
Publisher : Academic Press
Release : 2017-01-27
ISBN : 0128105356
Language : En, Es, Fr & De

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Book Description :

Proteomics in Biology, Part B, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in proteomics. Continues the legacy of this premier serial with quality chapters that focus on proteomics Contains contributions from leading authorities

Advances in Clinical Chemistry

Advances in Clinical Chemistry Book
Author : Anonim
Publisher : Academic Press
Release : 2015-04-27
ISBN : 012802464X
Language : En, Es, Fr & De

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Book Description :

Volume 69 in the internationally acclaimed Advances in Clinical Chemistry contains chapters authored by world renowned clinical laboratory scientists, physicians and research scientists. The serial provides the latest and most up-to-date technologies related to the field of Clinical Chemistry and is the benchmark for novel analytical approaches in the clinical laboratory. Expertise of international contributors Latest cutting-edge technologies Comprehensive in scope

Chemical Analysis of Food

Chemical Analysis of Food Book
Author : Yolanda Pico
Publisher : Academic Press
Release : 2020-06-16
ISBN : 0128132671
Language : En, Es, Fr & De

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Book Description :

Chemical Analysis of Food: Techniques and Applications, Second Edition, reviews the latest technologies and challenges in all stages of food analysis, from selecting the right approach, how to perform analytic procedures, and how to measure and report the results. The book is structured in two parts: the first describes the role of the latest developments in analytical and bio-analytical techniques, with the second reviewing innovative applications and issues in food analysis. The techniques discussed range from the non-invasive and non-destructive, such as infrared spectroscopy and ultrasound, to newly emerging areas, such as nanotechnology, biosensors and electronic noses and tongues. This thoroughly updated edition includes new chapters on ambient mass spectrometry, imaging techniques, omics approaches in food analysis, natural toxins analysis, food contact materials, nanomaterials and organic foods. All chapters are updated or rewritten to bring the content completely up-to-date. Reviews the attributes, benefits, limits and potential of all relevant analytic modalities, including spectroscopy, ultrasound and nanotechnology applications Provides in-depth coverage of each technology, including near-infrared, mid-infrared, and Raman spectroscopy, low intensity ultrasound, microfluidic devices and biosensors, electronic noses and tongues, mass spectrometry and molecular techniques Outlines practical solutions to challenging problems in food analysis, including how to combine techniques for improved efficacy Covers all relevant applications of food analysis, such as traceability, authenticity and fraud, biologically-active food components, novel food and nutritional supplements, flavors and fragrances, and contaminants and allergens Provides researchers with a single source of current research and includes contributions from internationally renowned experts in food science and technology and nutrition

Proteome Analysis

Proteome Analysis Book
Author : David W Speicher
Publisher : Elsevier
Release : 2004-04-09
ISBN : 9780444510242
Language : En, Es, Fr & De

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Book Description :

State-of-the-art protein profiling methods include high resolution two-dimensional gels, two-dimensional differential in-gel electrophoresis, LC-MS and LC-MS/MS using accurate mass tags, and protein identifications of proteins from gels using mass spectrometry methods are discussed in depth. Other chapters describe comprehensive characterization of proteomes using electrophoretic prefractionation and analyses of sub-proteomes based on specific posttranslational modifications including the phospho-proteome, the glyco-proteome, and nitrated proteins. These conventional proteome analysis chapters are complemented by discussion of emerging technologies and approaches such as affinity based biosensor proteomics as well as the use of protein microarrays, microfluidics and nanotechnology. Strategies for improving throughput by automation are also discussed.-

Natural Products Analysis

Natural Products Analysis Book
Author : Vladimir Havlicek,Jaroslav Spizek
Publisher : John Wiley & Sons
Release : 2014-09-17
ISBN : 1118876024
Language : En, Es, Fr & De

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Book Description :

This book highlights analytical chemistry instrumentation andpractices applied to the analysis of natural products and theircomplex mixtures, describing techniques for isolating andcharacterizing natural products. • Applies analytical techniques to natural productsresearch – an area of critical importance to drugdiscovery • Offers a one-stop shop for most analytical methods:x-ray diffraction, NMR analysis, mass spectrometry, and chemicalgenetics • Includes coverage of natural products basics andhighlights antibacterial research, particularly important asefforts to combat drug resistance gain prominence • Covers instrumental techniques with enough detail forboth current practitioners and beginning researchers