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Monoclonal Antibodies

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Monoclonal Antibody and Immunosensor Technology

Monoclonal Antibody and Immunosensor Technology Book
Author : A.M. Campbell
Publisher : Elsevier
Release : 1991-12-12
ISBN : 9780080887357
Language : En, Es, Fr & De

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Book Description :

This highly practical book, and successor to Volume 13 in the Laboratory Techniques series, explores further and provides more comprehensive, autoritative information on the production of Mabs. Much new and illuminating material has been included covering the concepts behind the application of recombinant DNA technology and biosensor technology to monoclonal antibodies, and all the human Mab technology facilitated by PCR of antibody genes. Also included in this latest volume is a section focussing on other methods of obtaining B cell clones such as short-term culture and oncogene transformation and an interesting section on Mab patents.

Monoclonal Antibodies

Monoclonal Antibodies Book
Author : Johann Hinrich Peters
Publisher : Springer Verlag
Release : 1992
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Monoclonal Antibodies book written by Johann Hinrich Peters, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Trends in Monoclonal Antibody Research

Trends in Monoclonal Antibody Research Book
Author : Marie A. Simmons
Publisher : Nova Publishers
Release : 2005
ISBN : 9781594545504
Language : En, Es, Fr & De

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Book Description :

This new and important international source of information brings together leading-edge research dedicated to monoclonal antibodies. Monoclonal antibodies (MABs) are: antibodies of exceptional purity and specificity; components of the immune system; able to recognise and bind to a specific antigen. Monoclonal antibodies are currently utilised in many diagnostic procedures, including: measuring protein and drug levels in serum; typing tissue and blood; identifying infectious agents; identifying clusters of differentiation for the classification and follow-up therapy of leukaemias and lymphomas; identifying tumour antigens and auto-antibodies; identifying the specific cells involved in the immune response; identifying and quantifying hormones. For example, monoclonal antibodies (MABs or MOABs) work on cancer cells in the same way natural antibodies work, by identifying and binding to the target cells. They then alert other cells in the immune system to the presence of the cancer cells. MABs are specific for a particular antigen-one designed for a B-cell lymphoma will not work on cells for ovarian cancer cells for example.

Monoclonal Antibodies

Monoclonal Antibodies Book
Author : Vincent Ossipow,Nicolas Fischer
Publisher : Humana Press
Release : 2016-08-23
ISBN : 9781493963225
Language : En, Es, Fr & De

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Book Description :

Monoclonal Antibodies: Methods and Protocols, Second Edition expands upon the previous edition with current, detailed modern approaches to isolate and characterize monoclonal antibodies against carefully selected epitopes. This edition includes new chapters covering the key steps to generate high quality monoclonals via different methods, from antigen generation to epitope mapping and quality control of the purified IgG. Chapters are divided into four parts corresponding to four distinct objectives. Part I covers monoclonal antibody generation, Part II deals with monoclonal antibody expression and purification, Part III presents methods for monoclonal antibody characterization and modification, and Part IV describes selected applications of monoclonal antibodies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Monoclonal Antibodies: Methods and Protocols, Second Edition provides crucial initial steps of monoclonal antibody generation and characterization with state-of-the art protocols.

Monoclonal Antibodies

Monoclonal Antibodies Book
Author : Roger H. Kennett
Publisher : Springer
Release : 2012-03-30
ISBN : 9781461575078
Language : En, Es, Fr & De

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Book Description :

On August 7, 1975, Kohler and Milstein published in Nature (256:495) a report describing "Continuous cultures of fused cells secreting antibody of predefined specificity. " Their report has become a classic and has already had a profound effect on basic and applied research in biology and medicine. By the time the first Workshop on Lymphocyte Hybridomas (Current Topics in Microbiology and Im munology 81, 1978) was held on April 3-5, 1978, in Bethesda, Maryland, investi gators from many laboratories had made hybrids between plasmacytomas and spleen cells from immunized animals and had obtained monoclonal antibodies reacting with a broad variety of antigenic determinants. At the time Kohler and Milstein introduced this new technology, the editors of this volume were involved in the production of antisera against differentiation antigens (K. B. B. ), histocompatibility antigens (T. ]. McK. ), and human tumor associated antigens (R. H. K. ). Because of the potential usefulness of monoclonal antibodies in these areas, we each began production of hybridomas and analysis of the resulting monoclonal reagents. One of the most interesting aspects of participation in the early stages of the development and application of hybrid oma technology has been observing how the implications of the initial observa tions gradually spread first among the practitioners of immunology and immu nogenetics, and then to other areas of the biological sciences, such as developmental biology, biochemistry, human genetics, and cell and tumor biology.

A Practical Guide to Monoclonal Antibodies

A Practical Guide to Monoclonal Antibodies Book
Author : J. Eryl Liddell,A. Cryer
Publisher : John Wiley & Sons
Release : 1991-08-26
ISBN : 9780471929055
Language : En, Es, Fr & De

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Book Description :

Includes all of the information required to produce monoclonal antibodies in the laboratory and to prepare them for use in a multitude of given applications. Production procedures are treated in chronological order, beginning with basic tissue culture techniques, immunization strategies and screening test design, followed by production of hybridoma cell lines and basic antibody characterization, purification and labeling. Each chapter contains explanatory text on each step with comparative analysis of methods where appropriate. All necessary experimental protocols are presented in a self-contained format that is easy to follow in the laboratory. Alternative protocols are provided where relevant; for others not included in full, source references are presented. Surveys the current status of human hybridoma production and antibody engineering using molecular biology techniques.

Applications And Engineering Of Monoclonal Antibodies

Applications And Engineering Of Monoclonal Antibodies Book
Author : David J. King
Publisher : CRC Press
Release : 1998-11-27
ISBN : 9780748404223
Language : En, Es, Fr & De

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Book Description :

A valuable resource for researchers and workers in the fields of both pharmaceuticals and biotechnology as well as undergraduates in biochemistry, applied biology, biomedical sciences and pharmacy, this book compares established techniques of antibody production with the new. Antibody structure and the implications of antibody engineering are fully discussed, and a case study approach illustrates how antibodies are finding increasing use in the diagnosis and treatment of disease. The volume ends with commercial expression, purification and large-scale manufacture of antibodies and their future potential, particularly as therapeutic agents.

The Lock and Key of Medicine

The Lock and Key of Medicine Book
Author : Lara Marks
Publisher : Yale University Press
Release : 2015-01-01
ISBN : 0300167733
Language : En, Es, Fr & De

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Book Description :

This book is the first to tell the extraordinary yet unheralded history of monoclonal antibodies, or Mabs. Though unfamiliar to most nonscientists, these microscopic protein molecules are everywhere, quietly shaping our lives and healthcare. They have radically changed understandings of the pathways of disease, enabling faster, cheaper, and more accurate clinical diagnostic testing. And they lie at the heart of the development of genetically engineered drugs such as interferon and blockbuster personalized therapies such as Herceptin. Lara V. Marks recounts the risks and opposition that a daring handful of individuals faced while discovering and developing Mabs, and she addresses the related scientific, medical, technological, business, and social challenges that arose. She offers a saga of entrepreneurs who ultimately changed the healthcare landscape and brought untold relief to millions of patients. Even so, controversies over Mabs remain, which the author explores through the current debates on their cost-effectiveness.

Monoclonal Antibodies

Monoclonal Antibodies Book
Author : Philip Shepherd,Christopher J. Dean
Publisher : Practical Approach (Paperback)
Release : 2000
ISBN : 9780199637225
Language : En, Es, Fr & De

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Book Description :

Monoclonal Antibodies: A Practical Approach covers the preparation, testing, derivation, and applications of monoclonal antibodies. New immunological techniques incorporating tried and tested methodologies are described, making the book of interest to established and inexperienced immunologists. Both the standard somatic hybridization technique and recombinant techniques, including the use of phage libraries, for the preparation of rodent and human monoclonal antibodies are described. Protocols for both the small and large scale production are detailed, as well as purification and labelling (with both radioisotopes and non-radioisotopes) methods. The applications of monoclonal antibodies in immunoblotting, enzyme linked immunoassays, immunofluorescence, and FACS analysis are all covered in detail. Finally protocols are given for the use of monoclonal antibodies in rheumatoid arthritis, tissue typing, detecting DNA modified during chemotherapy, and in the clinical analysis of transplantation samples for malignancy. This book will therefore be an invaluable laboratory companion to anyone using monoclonal antibodies in their research.

Monoclonal Antibodies

Monoclonal Antibodies Book
Author : H. Zola
Publisher : Springer
Release : 2000
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Monoclonal antibodies are highly specific reagents that play a crucial role in medical and biological research, and promise to yield major therapeutic advances. This book provides detailed coverage of the 'classical' methods of antibody production, including hybridization and cloning, and describes the latest techniques for genetically engineering antibodies and their derivatives. The major applications and the selection of the most appropriate anti-bodies for particular applications are also discussed.

Monoclonal Antibodies in Headache

Monoclonal Antibodies in Headache Book
Author : Antoinette Maassen van den Brink,Paolo Martelletti
Publisher : Springer
Release : 2021-05-07
ISBN : 9783030690311
Language : En, Es, Fr & De

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Book Description :

Intended to promote a more appropriate and modern therapeutic approach to migraine management, this book is the first to deal with monoclonal antibodies in this context. Authored by the most respected migraine experts from around the globe and drawing on the lessons learned in both clinical trials and clinical practice, it reviews the current state of knowledge on this important therapeutic innovation, which has produced impressive data in randomized controlled trials, and the efficacy and safety of which have been confirmed in day-to-day real-world use. Given its scope, the book will appeal to a broad range of specialists, including pharmacologists, clinical pharmacologists, neurologists and internists, but also to residents and medical students.

Monoclonal Antibodies and Functional Cell Lines

Monoclonal Antibodies and Functional Cell Lines Book
Author : Roger H. Kennett
Publisher : Springer Science & Business Media
Release : 2013-03-08
ISBN : 1468446738
Language : En, Es, Fr & De

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Book Description :

This volume serves as a follow-up to our previous book, MonoclonalAntibodies Hybridomas: A New Dimension in Biological Analyses. We continue the theme of monoclonal antibodies and their applications, attempting to cover some of the areas not covered in the previous volume. We again include an appendix de scribing methods useful to those who ar-e beginning to apply these techniques in their own laboratories. This volume will be followed by another concentrating on the combination of monoclonal antibody techniques with molecular genetic techniques to study structure/function relationships at the level of both the gene and gene product. Roger H. Kennett Kathleen B. Bechtol Philadelphia, Pennsylvania Thomas J. McKearn Princeton, New Jersey IX Acknowledgments Roger Kennett acknowledges the patience and support of his wife, Carol, and his family, friends, and colleagues during the work on this volume, and again thanks, above all, the Lord, Jesus Christ. Kathleen Bechtol wishes to thank colleagues and friends for their support and understanding during the months of preparation of this volume. Tom McKearn acknowledges and thanks his wife, Pat, and his family for their support and encouragement. Xl Contents PART I INTRODUCTION 1 Introduction: Reflections on Nine Years of Monoclonal Antibodies from Hybridomas 3 ROGER H. KENNETT, KATHLEEN B. BECHTOL, AND THOMAS J. McKEARN 1. Biotechnology'S "Coming of Age". . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 II. Monoclonal Antibodies-An Overview of Applications. . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 III. Commercialization of Monoclonal Antibody Technology.. . . .. ... . .... .... .. . ... . . 10 References. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 . . . . . . . . . . . . . . . . . . . . .

Monoclonal Antibodies

Monoclonal Antibodies Book
Author : James W. Goding
Publisher : Unknown
Release : 1986
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

This book represents the distillation and critical evaluation of many hundreds of publications relating to the production and use of antibodies. Therefore it is restricted to the "core" techniques of production and handling of antibodies, and their use in studies of antigen analysis, purification and localization.

Human Monoclonal Antibodies

Human Monoclonal Antibodies Book
Author : Michael Steinitz
Publisher : Humana Press
Release : 2019-02-04
ISBN : 9781493989577
Language : En, Es, Fr & De

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Book Description :

This second edition volume expands on the previous edition with descriptions of recent developments in the field. The chapters in this book cover topics such as monoclonal antibodies for the treatment of melanoma; production and purification of human monoclonal antibodies; humanization and optimization of monoclonal antibodies; rapid chimerization of monoclonal antibodies; epitope mapping via phage display from single gene libraries; recombinant antibodies made by combining phage and yeast display selections; production of stabilized antibody fragments in the E. coli bacterial cytoplasm and transfected mammalian cells; and analysis of CAR T cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Unique and thorough, Human Monoclonal Antibodies: Methods and Protocols, Second Edition is a valuable tool for novice and expert researchers interested in learning more about this evolving field.

Monoclonal Antibodies

Monoclonal Antibodies Book
Author : Steven Shire
Publisher : Woodhead Publishing
Release : 2015-04-24
ISBN : 0081002971
Language : En, Es, Fr & De

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Book Description :

Monoclonal antibodies (MAbs) are currently the major class of protein bio therapeutic being developed by biotechnology and pharmaceutical companies. Monoclonal Antibodies discusses the challenges and issues revolving around development of a monoclonal antibody produced by recombinant DNA technology into a therapeutic agent.This book covers downstream processing which includes design of processes to manufacture the formulation, formulation design, fill and finish into closure systems and routes of administration. The characterization of the final drug product is covered where the use of biophysical methods combined with genetic engineering is used to understand the solution properties of the formulation. The latter has become very important since many indications such as arthritis and asthma require the development of formulations for subcutaneous delivery (SC). The development of formulations for IV delivery is also important and comes with a different set of challenges. The challenges and strategies that can overcome these limitations are discussed in this book, starting with an introduction to these issues, followed by chapters detailing strategies to deal with them. Subsequent chapters explore the processing and storage of mAbs, development of delivery device technologies and conclude with a chapter on the future of mAbs in therapeutic remedies. Discusses the challenges to develop MAbs for intravenous (IV) and subcutaneous delivery (SC) Presents strategies to meet the challenges in development of MAbs for SC and IV administration Discusses the use of biophysical analytical tools coupled with MAb engineering to understand what governs MAb properties at high concentration

Monoclonal Antibody Protocols

Monoclonal Antibody Protocols Book
Author : William C. Davis
Publisher : Springer Science & Business Media
Release : 1995-05-23
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Monoclonal Antibody Protocols provides researchers in biomedical, agricultural, and biological science with a set of detailed, easy-to-follow methods for developing and using monoclonal antibodies. The protocols emphasize techniques that optimize the outgrowth of hybridomas from primary cultures of fused cells and the use of an alternative, electric-field-mediated cell fusion technique to increase the yield of hybridomas. The book stresses antibodies produced in mice, but includes methods of producing xenogeneic hybrids that yield human, bovine, equine, and porcine monoclonal antibodies. With its detailed instructions, its comments on how to alter the various steps of a protocol in order to accommodate different materials, and its hints and tips that often make the difference between success and failure, Monoclonal Antibody Protocols provides you with a ready and indispensable source of information for preparing and using monoclonal antibodies successfully in your laboratory.

Radiolabeled Monoclonal Antibodies for Imaging and Therapy

Radiolabeled Monoclonal Antibodies for Imaging and Therapy Book
Author : Suresh C. Srivastava
Publisher : Springer
Release : 2013-01-18
ISBN : 9781468455403
Language : En, Es, Fr & De

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Book Description :

The advent of hybridoma technology leading to the successful produc tion of monoclonal antibodies against a variety of tumor-associated antigens has, during the last decade, provided a very powerful tool for research and clinical investigations. These highly specific reagents have essentially replaced the polysera of the earlier days. The successful demonstration of the many wide ranging capabilities of the monoclonal antibody technique has already begun to exert an enormous impact on diverse areas of research in basic science and medicine. In particular, the potential of monoclonal antibodies to serve as carriers for selective targeting of radionuclides to tumors for diagnosis or therapy, has stimulated an intense surge of research interest and even revived hopes of realizing Ehrlich's concept of the "magic bullet". Indeed, the technology appears to be on the threshold of a revolution in diagnosing and treating malignant disease. Much work remains to be done, however, and even though the progress has been impressive, results to date have shown only moderate success. There is no question that the limited success we have achieved thus far is merely a prelude to the many more exciting developments yet to come.

Monoclonal Antibodies

Monoclonal Antibodies Book
Author : Heddy Zola
Publisher : CRC Press
Release : 2013-11-20
ISBN : 1482242680
Language : En, Es, Fr & De

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Book Description :

This book describes, in detail, tested techniques for the produc-tion and use of monoclonal antibodies. It covers those aspects of interest to all scientists working with monoclonal antibodies and presents methods in a step-by-step format for easy refer-ence. The text serves as a laboratory manual; and discusses rationale behind each method, and th

The Pharmacology of Monoclonal Antibodies

The Pharmacology of Monoclonal Antibodies Book
Author : Martin Rosenberg,Gordon P. Moore
Publisher : Springer Science & Business Media
Release : 2012-12-06
ISBN : 3642784321
Language : En, Es, Fr & De

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Book Description :

A sample of the most exciting developments in the cloning, manipulation, expression and application of genetically-engineered monoclonal antibodies. This rapidly-evolving field has witnessed the PCR combinatorial cloning of vast immunological diversity, in vitro mutagenesis of MAbs, MAbs created by transgenic animals, novel expression systems in plants, animals and lower systems, as well as a rich variety of genetically modified MAbs as potential therapeutic agents. Leading scientists from academia and industry present their own findings as well as short reviews of these research areas.

Monoclonal Antibody Technology The Production and Characterization of Rodent and Human Hybridomas

Monoclonal Antibody Technology  The Production and Characterization of Rodent and Human Hybridomas Book
Author : A.M. Campbell
Publisher : Elsevier
Release : 2000-04-01
ISBN : 9780080858821
Language : En, Es, Fr & De

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Book Description :

This volume contains detailed, comprehensive advice on rat, mouse and human hybridoma production. It begins with a general introduction, then describes the practical applications of the technology with photographs and protocols for everything from animal dissection to epitope analysis of antigens.