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Molecular Biology Techniques

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Molecular Biology Techniques

Molecular Biology Techniques Book
Author : Sue Carson,Heather Miller,Melissa C. Srougi,D. Scott Witherow
Publisher : Academic Press
Release : 2019-03-05
ISBN : 0128157755
Language : En, Es, Fr & De

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Book Description :

Molecular Biology Techniques: A Classroom Laboratory Manual, Fourth Edition is a must-have collection of methods and procedures on how to create a single, continuous, comprehensive project that teaches students basic molecular techniques. It is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology—or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students will gain hands-on experience on subcloning a gene into an expression vector straight through to the purification of the recombinant protein. Presents student-tested labs proven successful in real classroom laboratories Includes a test bank on a companion website for additional testing and practice Provides exercises that simulate a cloning project that would be performed in a real research lab Includes a prep-list appendix that contains necessary recipes and catalog numbers, providing staff with detailed instructions

Molecular Biology Techniques

Molecular Biology Techniques Book
Author : Sue Carson,Susan Carson,Heather Miller,D. Scott Witherow
Publisher : Academic Press
Release : 2012
ISBN : 0123855446
Language : En, Es, Fr & De

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Book Description :

This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project" approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions

Techniques for Molecular Biology

Techniques for Molecular Biology Book
Author : D Tagu,C Moussard
Publisher : Science Publishers
Release : 2006-01-04
ISBN : 9781578083619
Language : En, Es, Fr & De

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Book Description :

With the underpinning role of forage legumes in the nitrogen economy and animal productivity from temperate grasslands certain to expand in the future, particularly in regions where their potential has not yet been realized, it is essential that the wealth of information currently available is widely disseminated. This book serves the purpose with very detailed information on and illustrations of 35 selected forage legume species that will contribute to more efficient and viable grassland farming.

Morphology Methods

Morphology Methods Book
Author : Ricardo V. Lloyd
Publisher : Springer Science & Business Media
Release : 2001-06-15
ISBN : 1592591906
Language : En, Es, Fr & De

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Book Description :

The past several decades have witnessed an impressive array of conceptual and techno logical advances in the biomedical sciences. Much of the progress in this area has developed directly as a result of new morphology-based methods that have permitted the assessment of chemical, enzymatic, immunological, and molecular parameters at the cellular and tissue levels. Additional novel approaches including laser capture microdissection have also emerged for the acquisition of homogeneous cell popula tions for molecular analyses. These methodologies have literally reshaped the approaches to fundamental biological questions and have also had a major impact in the area of diagnostic pathology. Much of the groundwork for the development of morphological methods was estab th lished in the early part of the 19 century by Francois-Vincent Raspail, generally acknowledged as the founder of the science of histochemistry. The earliest work in the field was primarily in the hands of botanists and many of the approaches to the under standing of the chemical composition of cells and tissues involved techniques such as microincineration, which destroyed structural integrity. The development of aniline th dyes in the early 20 century served as a major impetus to studies of the structural rather than chemical composition of tissue. Later in the century, however, the focus returned to the identification of chemical constituents in the context of intact cell and tissue structure.

Molecular Biology Techniques

Molecular Biology Techniques Book
Author : Walt Ream,Katharine G. Field
Publisher : Academic Press
Release : 1998-11-17
ISBN : 0080536824
Language : En, Es, Fr & De

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Book Description :

This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. Key Features * Provides well-tested experimental protocols for each technique * Lists the reagents and preparation of each experiment separately * Contains a complete schedule of experiments and the preparation required * Includes study questions at the end of each chapter

Principles and Techniques of Biochemistry and Molecular Biology

Principles and Techniques of Biochemistry and Molecular Biology Book
Author : Keith Wilson,John Walker
Publisher : Cambridge University Press
Release : 2010-03-04
ISBN : 1107393493
Language : En, Es, Fr & De

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Book Description :

This best-selling undergraduate textbook provides an introduction to key experimental techniques from across the biosciences. It uniquely integrates the theories and practices that drive the fields of biology and medicine, comprehensively covering both the methods students will encounter in lab classes and those that underpin recent advances and discoveries. Its problem-solving approach continues with worked examples that set a challenge and then show students how the challenge is met. New to this edition are case studies, for example, that illustrate the relevance of the principles and techniques to the diagnosis and treatment of individual patients. Coverage is expanded to include a section on stem cells, chapters on immunochemical techniques and spectroscopy techniques, and additional chapters on drug discovery and development, and clinical biochemistry. Experimental design and the statistical analysis of data are emphasised throughout to ensure students are equipped to successfully plan their own experiments and examine the results obtained.

Wood Formation in Trees

Wood Formation in Trees Book
Author : Nigel J Chaffey
Publisher : CRC Press
Release : 2002-01-10
ISBN : 9780203166444
Language : En, Es, Fr & De

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Book Description :

Trees are a major component of the biosphere and have played an important part in the world's history and culture. With the modern challenges of global warming and dwindling fossil fuel reserves, trees, and in particular their wood, can provide solutions. Unfortunately, too little is known about the biology of these plants, due largely to a lack of

Techniques in Molecular Biology

Techniques in Molecular Biology Book
Author : J.M. Walker,W. Gaastra
Publisher : Springer Science & Business Media
Release : 2012-12-06
ISBN : 9401165637
Language : En, Es, Fr & De

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Book Description :

The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly introduced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have contributed a wide range of new techniques. The purpose of this book therefore is to introduce the reader to a selection of the more advanced analytical and preparative techniques which the editors consider to be frequently used by research workers in the field of molecular biology. In choosing techniques for this book we have obviously had to be selective, and for the sake of brevity a knowledge of certain basic biochemical techniques and terminology has been assumed. However, since many areas of molecular biology are developing at a formidable rate and constantly generating new terminology, a glossary of terms has been included. The techniques chosen for this book are essentially based on those used in a series of workshops on 'techniques in molecular biology' that have been held at The Hatfield Polytechnic in recent years. In choosing these chapters we have taken into account many useful suggestions and observations made by participants at these workshops. Each chapter aims to describe both the theory and relevant practical details for a given technique, and to identify both the potential and limitations of the technique. Each chapter is written by authors who regularly use the technique in their own laboratories.

Exam Prep for Molecular Biology Techniques

Exam Prep for  Molecular Biology Techniques Book
Author : Anonim
Publisher : Unknown
Release : 2021-05-13
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Exam Prep for Molecular Biology Techniques book written by , available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Basic Techniques in Molecular Biology

Basic Techniques in Molecular Biology Book
Author : Stefan Surzycki
Publisher : Springer Science & Business Media
Release : 2012-12-06
ISBN : 3642569684
Language : En, Es, Fr & De

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Book Description :

This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.

Basic Techniques in Biochemistry and Molecular Biology

Basic Techniques in Biochemistry and Molecular Biology Book
Author : R. K. Sharma
Publisher : I. K. International Pvt Ltd
Release : 2008-11-15
ISBN : 819074626X
Language : En, Es, Fr & De

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Book Description :

Fundamentals of biochemistry and molecular biology is an important component of all disciplines of Biology. In the era of multidisciplinary approach, the basic techniques in Biochemistry and Molecular Biology are much needed by the students of Botany, Zoology, Microbiology, Biotechnology, Fisheries, Veterinary, Pharmacology, Physiology, Medicine, Genetics, Agriculture and allied subjects both at undergraduate and postgraduate levels. This book includes 15 chapters covering more than 135 experimental protocols. It discussed all the relevant topics like pH and buffers, spectrophotometry, chromatography, carbohydrates, lipids, proteins, electrophoresis, enzyme immunology, vitamins and pigments, metabolites and molecular biology. It includes a wide range of experiments from preparation of culture media to PCR, Southern and Western blotting. All the experiments have been meticulously designed and special care has been taken to the safety in laboratory and precautions are given wheresoever required.

Laboratory Techniques in Biochemistry and Molecular Biology

Laboratory Techniques in Biochemistry and Molecular Biology Book
Author : Roy Hunter Burdon,P. H. van Knippenberg
Publisher : Unknown
Release : 1986
ISBN : 9780444807373
Language : En, Es, Fr & De

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Book Description :

Download Laboratory Techniques in Biochemistry and Molecular Biology book written by Roy Hunter Burdon,P. H. van Knippenberg, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Analytical Techniques in Biochemistry and Molecular Biology

Analytical Techniques in Biochemistry and Molecular Biology Book
Author : Rajan Katoch
Publisher : Springer Science & Business Media
Release : 2011-07-19
ISBN : 9781441997852
Language : En, Es, Fr & De

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Book Description :

Advances in biochemistry now allow us to control living systems in ways that were undreamt of a decade ago. This volume guides researchers and students through the full spectrum of experimental protocols used in biochemistry, plant biology and biotechnology.

Molecular Biology Techniques

Molecular Biology Techniques Book
Author : Source Wikipedia
Publisher : University-Press.org
Release : 2013-09
ISBN : 9781230545653
Language : En, Es, Fr & De

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Book Description :

Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online. Pages: 92. Chapters: Polymerase chain reaction, Plasmid, Southern blot, Northern blot, Gene knockout, Western blot, Cell culture, DNA sequencing, DNA microarray, Immunoprecipitation, Transmission electron microscopy DNA sequencing, Methods to investigate protein-protein interactions, Exome sequencing, ChIP-on-chip, Forster resonance energy transfer, Nucleic acid structure determination, Chromatin immunoprecipitation, Eastern blotting, ChIA-PET, COLD-PCR, Paired-end Tags, Combined bisulfite restriction analysis, Primer dimer, Kodecyte, DNA footprinting, Suspension array technology, PBR322, Allele-specific oligonucleotide, Chip-Sequencing, Streptamer, Oligomer restriction, Reverse transfection, Strep-tag, Selection and amplification binding assay, Knockout moss, Native PAGE, Isoelectric focusing, Helicase-dependent amplification, Subcloning, Plant transformation vector, Chemically defined medium, Magnet-assisted transfection, Branched DNA assay, TA cloning, Nuclease protection assay, Bio-Layer Interferometry, Southwestern blot, Synchronous culture, MassTag-PCR, Far-Eastern blotting, Ribosomal Intergenic Spacer analysis, Ligase chain reaction, Isopeptag, Far-western blotting, Gene knockin, Baby hamster kidney cell, Univec, VectorDB, Chromogenic in situ hybridization, Alanine scanning, Calcium chloride transformation, Fluorophore assisted carbohydrate electrophoresis, Reverse northern blot, Gel Doc, Racker unit, Promoter bashing, PBLU.

Algorithms in Computational Molecular Biology

Algorithms in Computational Molecular Biology Book
Author : Mourad Elloumi,Albert Y. Zomaya
Publisher : John Wiley & Sons
Release : 2011-04-04
ISBN : 1118101987
Language : En, Es, Fr & De

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Book Description :

This book represents the most comprehensive and up-to-date collection of information on the topic of computational molecular biology. Bringing the most recent research into the forefront of discussion, Algorithms in Computational Molecular Biology studies the most important and useful algorithms currently being used in the field, and provides related problems. It also succeeds where other titles have failed, in offering a wide range of information from the introductory fundamentals right up to the latest, most advanced levels of study.

Articles on Molecular Biology Techniques Including Northern Blot Polymerase Chain Reaction Plasmid Southern Blot Gene Knockout Western Blot DN

Articles on Molecular Biology Techniques  Including  Northern Blot  Polymerase Chain Reaction  Plasmid  Southern Blot  Gene Knockout  Western Blot  DN Book
Author : Hephaestus Books
Publisher : Unknown
Release : 2011-08-31
ISBN : 9781243242846
Language : En, Es, Fr & De

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Book Description :

Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online. Hephaestus Books represents a new publishing paradigm, allowing disparate content sources to be curated into cohesive, relevant, and informative books. To date, this content has been curated from Wikipedia articles and images under Creative Commons licensing, although as Hephaestus Books continues to increase in scope and dimension, more licensed and public domain content is being added. We believe books such as this represent a new and exciting lexicon in the sharing of human knowledge. This particular book is a collaboration focused on Molecular biology techniques.

Computational Methods in Molecular Biology

Computational Methods in Molecular Biology Book
Author : S.L. Salzberg,D.B. Searls,S. Kasif
Publisher : Elsevier
Release : 1998-06-19
ISBN : 9780080860930
Language : En, Es, Fr & De

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Book Description :

Computational biology is a rapidly expanding field, and the number and variety of computational methods used for DNA and protein sequence analysis is growing every day. These algorithms are extremely valuable to biotechnology companies and to researchers and teachers in universities. This book explains the latest computer technology for analyzing DNA, RNA, and protein sequences. Clear and easy to follow, designed specifically for the non-computer scientist, it will help biologists make better choices on which algorithm to use. New techniques and demonstrations are elucidated, as are state-of-the-art problems, and more advanced material on the latest algorithms. The primary audience for this volume are molecular biologists working either in biotechnology companies or academic research environments, individual researchers and the institutions they work for, and students. Any biologist who relies on computers should want this book. A secondary audience will be computer scientists developing techniques with applications in biology. An excellent reference for leading techniques, it will also help introduce computer scientists to the biology problems. This is an outstanding work which will be ideal for the increasing number of scientists moving into computational biology.

Pattern Recognition in Computational Molecular Biology

Pattern Recognition in Computational Molecular Biology Book
Author : Mourad Elloumi,Costas Iliopoulos,Albert Y. Zomaya,Jason T. L. Wang
Publisher : John Wiley & Sons
Release : 2015-12-29
ISBN : 1118893689
Language : En, Es, Fr & De

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Book Description :

A comprehensive overview of high-performance pattern recognition techniques and approaches to Computational Molecular Biology This book surveys the developments of techniques and approaches on pattern recognition related to Computational Molecular Biology. Providing a broad coverage of the field, the authors cover fundamental and technical information on these techniques and approaches, as well as discussing their related problems. The text consists of twenty nine chapters, organized into seven parts: Pattern Recognition in Sequences, Pattern Recognition in Secondary Structures, Pattern Recognition in Tertiary Structures, Pattern Recognition in Quaternary Structures, Pattern Recognition in Microarrays, Pattern Recognition in Phylogenetic Trees, and Pattern Recognition in Biological Networks. Surveys the development of techniques and approaches on pattern recognition in biomolecular data Discusses pattern recognition in primary, secondary, tertiary and quaternary structures, as well as microarrays, phylogenetic trees and biological networks Includes case studies and examples to further illustrate the concepts discussed in the book Pattern Recognition in Computational Molecular Biology: Techniques and Approaches is a reference for practitioners and professional researches in Computer Science, Life Science, and Mathematics. This book also serves as a supplementary reading for graduate students and young researches interested in Computational Molecular Biology. Mourad Elloumi, PhD, is Professor in Computer Science at the University of Tunis-El Manar, Tunisia. Dr. Elloumi is the author/co-author of more than 50 publications in international journals and conference proceedings related to Algorithmics, Computational Molecular Biology, and Knowledge Discovery and Data Mining. Costas S. Iliopoulos, PhD, is Professor of AlgorithmDesign at King's College London, UK. Dr. Iliopoulos co-authored over 300 peer-reviewed articles in pattern matching and combinatorics of strings. He serves on the editorial board of the Journal of Discrete Algorithms, Computer Mathematics & Combinatorial Computing, and System Biology & Biomedical Technologies. Jason T. L. Wang, PhD, is Professor of Computer Science at the New Jersey Institute of Technology, USA. Dr. Wang has published extensively on Data Mining and Computational Molecular Biology, and has been a member of program committees for over 200 conferences and workshops in these and related areas. Albert Y. Zomaya, PhD, is the Chair Professor of High Performance Computing & Networking in the School of Information Technologies, University of Sydney, Australia. Dr. Zomaya published more than 500 scientific papers and articles and is author, co-author or editor of more than 20 books. Dr. Zomaya is Fellow of AAAS, IEEE, and IET.

Molecular Biology

Molecular Biology Book
Author : Susan J. Karcher
Publisher : Academic Press
Release : 1995-11-28
ISBN : 9780080536835
Language : En, Es, Fr & De

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Book Description :

This course manual instructs students in recombinant DNA techniques and other essential molecular biology techniques in the context of projects. The project approach inspires and captivates students; it involves them in the scientific experience, providing continuity to laboratory bench time and an understanding of the principles underlying the techniques presented. Molecular Biology is a must for any department, operating under budgetary constraints that offers or plans to offer a course in molecular cloning. Includes a glossary of over 200 terms important for understanding molecular biology Uses an inexpensive source of eukaryotic cells - great for schools on a budget Includes Methods Locator that provides instant access to the latest methods Contain clearly written, easy-to-follow, student-tested instructions: Sterile techniques Phage titration Gel electrophoresis of DNA Restriction enzyme digestion Plasmid isolation Transformation of E. Coli Recombinant DNA cloning Nick translation labeling Nonradioactive primer labelling Nonradioactive DNA detection Southern blotting Colony hybridization Purification of plant DNA RNA purification Northern blotting Purification of poly A+ RNA Polymerase chain reaction (PCR)