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Microfluidic Cell Culture Systems

Microfluidic Cell Culture Systems Book
Author : Jeffrey T Borenstein,Vishal Tandon,Sarah L Tao,Joseph L. Charest
Publisher : Elsevier
Release : 2018-09-12
ISBN : 0128136723
Language : En, Es, Fr & De

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Book Description :

Techniques for microfabricating intricate microfluidic structures that mimic the microenvironment of tissues and organs, combined with the development of biomaterials with carefully engineered surface properties, have enabled new paradigms in and cell culture-based models for human diseases. The dimensions of surface features and fluidic channels made accessible by these techniques are well-suited to the size scale of biological cells. Microfluidic Cell Culture Systems applies design and experimental techniques used in in microfluidics, and cell culture technologies to organ-on-chip systems. This book is intended to serve as a professional reference, providing a practical guide to design and fabrication of microfluidic systems and biomaterials for use in cell culture systems and human organ models. The book covers topics ranging from academic first principles of microfluidic design, to clinical translation strategies for cell culture protocols. The goal is to help professionals coming from an engineering background to adapt their expertise for use in cell culture and organ models applications, and likewise to help biologists to design and employ microfluidic technologies in their cell culture systems. This 2nd edition contains new material that strengthens the focus on in vitro models useful for drug discovery and development. One new chapter reviews liver organ models from an industry perspective, while others cover new technologies for scaling these models and for multi-organ systems. Other new chapters highlight the development of organ models and systems for specific applications in disease modeling and drug safety. Previous chapters have been revised to reflect the latest advances. Provides design and operation methodology for microfluidic and microfabricated materials and devices for organ-on-chip disease and safety models. This is a rapidly expanding field that will continue to grow along with advances in cell biology and microfluidics technologies. Comprehensively covers strategies and techniques ranging from academic first principles to industrial scale-up approaches. Readers will gain insight into cell-material interactions, microfluidic flow, and design principles. Offers three fundamental types of information: 1) design principles, 2) operation techniques, and 3) background information/perspectives. The book is carefully designed to strike a balance between these three areas, so it will be of use to a broad range of readers with different technical interests and educational levels.

Multiplexed Microfabricated Cell Culture Device for Stem Cell Process Development

Multiplexed Microfabricated Cell Culture Device for Stem Cell Process Development Book
Author : M. Reichen
Publisher : Unknown
Release : 2012
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

In this thesis, a multiplexed micro-fabricated cell culture device is presented to probe the soluble micro-environment of stem cells. A large number of biological, physical and chemical variables determine the micro-environment of stem cells and affect therefore their fate. The soluble micro-environment is believed to play a pivotal role in controlling stem cell fate and its optimisation may therefore allow exploitation of applications such as regenerative medicine or drug discovery. However, novel tools are required to probe and optimise the soluble micro-environment. The ability to move small volumes of liquid and the minimal use of resources are important characteristics of microfluidic systems and thus, they are perfectly suited to study the micro-environment of stem cells. A microfluidic cell culture device must ful fil three important requirements to be of utility in stem cell bioprocess development. The first aspect addresses the need for adaptability and flexibility to implement changes in microfluidic designs to take account of the rapid progress in stem cell research. To this end, a packaging solution specifi cally designed for a microfluidic cell culture device has been developed. The packaging system has thus been complemented with a rapid fabrication method using a micro-milling machine to quickly fabricate disposable and easily reconfi gurable microfluidic chips. The packaging solution has a maximum burst pressure of approximately 7.5 bar and the fabrication method has a dimensional fidelity with less than 10% deviation from the nominal value. The second aspect focuses on the scalability and comparability of results and the feasibility of continuous culture of stem cells - both critical elements for the success of a microfluidic cell culture system for stem cell bioprocess development. A novel cell seeding method has been developed using a pipette to directly and carefully seed cells into a culture chamber within a microfluidic cell culture device. To prevent a wash-out of viable cells during continuous culture, a low hydrodynamic shear stress microfluidic chip has been developed. The cell culture device has been successfully tested using human embryonic stem cells (hESC) on feeder cells. The third aspect concerns the automated monitoring of stem cell bioprocesses in the cell culture device. A multiplexed micro fluidic bioreactor platform with time-lapse imaging has been developed to obtain data-rich experimental sets. The platform consisting of a cooled media reservoir and a pumping mechanism has been characterised and tested using mouse embryonic stem cells (mESC) as a proof of concept. The combination of these three aspects provides a basis towards a multiplexed microfabricated cell culture device, which allows data-rich experimentation and comparability with current benchscale culture vessels for stem cell expansion and di fferentiation.

Cardiac Cell Culture Technologies

Cardiac Cell Culture Technologies Book
Author : Zbigniew Brzozka,Elzbieta Jastrzebska
Publisher : Springer
Release : 2017-11-21
ISBN : 3319706853
Language : En, Es, Fr & De

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Book Description :

This book provides an introduction to the biological background of heart functioning and analyzes the various materials and technologies used for the development of microfluidic systems dedicated to cell culture, with an emphasis on cardiac cells. The authors describe the characterization of microfluidic systems for cardiac cell culture and center their discussion of the use of stem cell stimulation based on four different types: electrical, biochemical, physical, and mechanical. This book is appropriate for researchers focused on on-chip technologies and heart studies, students in bioengineering and microengineering courses, and a variety of professionals, such as biotechnologists, biomedical engineers, and clinicians working in the cardiac diseases field.

Microfluidic Cell Culture Systems

Microfluidic Cell Culture Systems Book
Author : Christopher Bettinger,Jeffrey T Borenstein,Sarah L Tao
Publisher : William Andrew
Release : 2012-12-31
ISBN : 143773460X
Language : En, Es, Fr & De

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Book Description :

The fields of microfluidics and BioMEMS are significantly impacting cell biology research and applications through the application of engineering solutions to human disease and health problems. The dimensions of microfluidic channels are well suited to the physical scale of biological cells, and the many advantages of microfluidics make it an attractive platform for new techniques in biology. This new professional reference applies the techniques of microsystems to cell culture applications. The authors provide a thoroughly practical guide to the principles of microfluidic device design and operation and their application to cell culture techniques. The resulting book is crammed with strategies and techniques that can be immediately deployed in the lab. Equally, the insights into cell culture applications will provide those involved in traditional microfluidics and BioMEMS with an understanding of the specific demands and opportunities presented by biological applications. The goal is to guide new and interested researchers and technology developers to the important areas and state-of-the-practice strategies that will enhance the efficiency and value of their technologies, devices and biomedical products. Provides insights into the design and development of microfluidic systems with a specific focus on cell culture applications Focuses on strategies and techniques for the design and fabrication of microfluidic systems and devices for cell culture Provides balanced coverage of microsystems engineering and bioengineering

Microfluidic Systems for Neuronal Cell Culture

Microfluidic Systems for Neuronal Cell Culture Book
Author : Alex Johnstone
Publisher : Unknown
Release : 2016
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Microfluidic Systems for Neuronal Cell Culture book written by Alex Johnstone, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Cardiac Cell Culture Technologies

Cardiac Cell Culture Technologies Book
Author : Zbigniew Brzozka,Elzbieta Jastrzebska
Publisher : Springer
Release : 2018-09-04
ISBN : 9783319889795
Language : En, Es, Fr & De

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Book Description :

This book provides an introduction to the biological background of heart functioning and analyzes the various materials and technologies used for the development of microfluidic systems dedicated to cell culture, with an emphasis on cardiac cells. The authors describe the characterization of microfluidic systems for cardiac cell culture and center their discussion of the use of stem cell stimulation based on four different types: electrical, biochemical, physical, and mechanical. This book is appropriate for researchers focused on on-chip technologies and heart studies, students in bioengineering and microengineering courses, and a variety of professionals, such as biotechnologists, biomedical engineers, and clinicians working in the cardiac diseases field.

Medical and Industrial Applications of Microfluidic based Cell Tissue Culture and Organs on a Chip Advances in Organs on a Chip and Organoids Technologies

Medical and Industrial Applications of Microfluidic based Cell Tissue Culture and Organs on a Chip  Advances in Organs on a Chip and Organoids Technologies Book
Author : Qasem Ramadan,Massimo Alberti,Martin Dufva,Yi-Chung Tung
Publisher : Frontiers Media SA
Release : 2019-10-16
ISBN : 2889631141
Language : En, Es, Fr & De

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Book Description :

Recent developments in microfluidics have demonstrated enormous potential of microscale cell culture for biology studies and recognized as instrumental in performing rapid and efficient experiments on small-sample volumes. Microfluidic-based cell culture is an area of research that keeps growing and gaining importance as a prominent technology, able to link scientific disciplines with industrial and clinical applications. In particular, organotypic cell culture and its integration in microfluidic devices would enable the realization of “in vivo-like” cell microenvironment within systems that are more amenable to automation and integration. Such remarkable advancement forms the foundation and motivation to transfer research from the laboratory to the field. Although the microfluidics and cell culture technologies have influenced many areas of science, significant research efforts are currently focus on finding methods to transform drug screening and toxicity testing from a system reliant on high-dose animal studies to one based primarily on human-relevant in vitro models. In line with regulatory developments precluding the use of animal testing, as well as fundamental differences in animal versus human, human in vitro methodologies are required to replace the animal-based testes while permitting physiologically relevant model equivalents for superior prediction. Organs-on-a-chip is an ambitious and rapidly growing technology that promise to bridge the gap between in vivo and in vitro studies and open wide possibilities in medical and industrial applications. However, many challenges are still ahead. This eBook present recent state-of-the-art works and critical reviews in organs-on-a-chip technology which highlight the new advances in this growing field with an emphasis on the interface between technological advancements and high impact applications.

Microfluidic Systems for Automatic Cell Culture and Precise Sampling

Microfluidic Systems for Automatic Cell Culture and Precise Sampling Book
Author : 黃俊維
Publisher : Unknown
Release : 2007
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Microfluidic Systems for Automatic Cell Culture and Precise Sampling book written by 黃俊維, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Studying Intercellular Signaling Underlying Human Diseases Using Open Microfluidic Coculture

Studying Intercellular Signaling Underlying Human Diseases Using Open Microfluidic Coculture Book
Author : Tianzi Zhang
Publisher : Unknown
Release : 2020
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

This dissertation focuses on the development of innovative open microfluidic cell culture platforms and their application in studying intercellular signaling underlying human diseases in controlled ex vivo microenvironments. Chapter 1 introduces the background of open microfluidic capillary systems, including the design considerations and current fabrication techniques, and addresses the advantages of using open microfluidic cell culture systems to study intercellular signaling. Chapter 2 presents a new open microfluidic capillary platform, which patterns biocompatible hydrogel walls along a rail insert set inside established cultureware. The permeable hydrogel walls provide segregation for the cells and support diffusion of soluble factors. Chapter 3 discusses a microscale collagen gel contraction assay with an engineered well plate insert that uses surface tension forces to load and manipulate small volumes of cell-laden collagen. The system is easily operated with two pipetting steps and the microscale device moves dynamically as a result of cellular forces. Chapter 4 presents an open microfluidic coculture platform consisting of two independent cell culture regions separated by a half wall. The cell types are selectively seeded into the regions and connected with cell culture media. Using the device, we show that human kidney tubular epithelial cells can tune organ-specificity in other endothelial cell types. Chapter 5 shows a preliminary asthma disease coculture model of human lung fibroblasts and primary alveolar macrophages using a similar open microfluidic coculture device to that described in Chapter 4. The preliminary cell culture result indicates that lung fibroblasts exhibit elevated cell contractility when cultured separately with alveolar macrophages in shared cell culture media. Chapter 6 concludes the thesis and proposes some future research directions and interesting metabolomic studies using existing or alternative open microfluidic systems.

Organs on chips for the pharmaceutical development process

Organs on chips for the pharmaceutical development process Book
Author : Jonas Christoffersson
Publisher : Linköping University Electronic Press
Release : 2019-01-22
ISBN : 9176853594
Language : En, Es, Fr & De

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Book Description :

Organs-on-chips are dynamic cell culture devices created with the intention to mimic organ function in vitro. Their purpose is to assess the toxicity and efficacy of drugs and, as early as possible in the pharmaceutical development process, predict the outcome of clinical trials. The aim of this thesis is to explain and discuss these cell culture devices from a design perspective and to experimentally exemplify some of the specific functions that characterize organs-on-chips. The cells in our body reside in complex environments with chemical and mechanical cues that affect their function and purpose. Such a complex environment is difficult to recreate in the laboratory and has therefore been overlooked in favor of more simple models, i.e. static twodimensional (2D) cell cultures. Numerous recent reports have shown cell culture systems that can resemble the cell’s natural habitat and enhance cell functionality and thereby potentially provide results that better reflects animal and human trials. The way these organs-on-chips improve in vitro cell culture assays is to include e.g. a three-dimensional cell architecture (3D), mechanical stimuli, gradients of oxygen or nutrients, or by combining several relevant cell types that affect each other in close proximity. The research conducted for this thesis shows how cells in 3D spheroids or in 3D hydrogels can be cultured in perfused microbioreactors. Furthermore, a pump based on electroosmosis, and a method for an objective conceptual design process, is introduced to the field of organs-on-chips.

Development of Microfluidic Systems for Micro scale Animal Cell Culture from Cell Separation Microencapsulation Micro dispensing to Perfusion 3 dimensional Cell Culture

Development of Microfluidic Systems for Micro scale Animal Cell Culture  from Cell Separation  Microencapsulation  Micro dispensing to Perfusion 3 dimensional Cell Culture Book
Author : 黃菘斌
Publisher : Unknown
Release : 2010
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Development of Microfluidic Systems for Micro scale Animal Cell Culture from Cell Separation Microencapsulation Micro dispensing to Perfusion 3 dimensional Cell Culture book written by 黃菘斌, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Cell Analysis on Microfluidics

Cell Analysis on Microfluidics Book
Author : Jin-Ming Lin
Publisher : Springer
Release : 2017-10-25
ISBN : 9811053944
Language : En, Es, Fr & De

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Book Description :

This book presents a detailed overview of the design, formatting, application, and development of microfluidic chips in the context of cell biology research, enumerating each element involved in microfluidics-based cell analysis, discussing its history, status quo, and future prospects, It also offers an extensive review of the research completed in the past decade, including numerous color figures. The individual chapters are based on the respective authors' studies and experiences, providing tips from the frontline to help researchers overcome bottlenecks in their own work. It highlights a number of cutting-edge techniques, such as 3D cell culture, microfluidic droplet technique, and microfluidic chip-mass spectrometry interfaces, offering a first-hand impression of the latest trends in the field and suggesting new research directions. Serving as both an elementary introduction and advanced guidebook, the book interests and inspires scholars and students who are currently studying microfluidics-based cell analysis methods as well as those who wish to do so.

Small Volume Cell Cultures

Small Volume Cell Cultures Book
Author : Pantea Gheibi
Publisher : Unknown
Release : 2017
ISBN : 9780355150483
Language : En, Es, Fr & De

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Book Description :

In vitro cell cultures are used widely in cell biology, drug discovery and toxicology. In the past two decades, there has been a steady shift in focus from simplistic 2D monolayer cultures to more sophisticated cultures introducing heterotypic cellular interactions, mechanical cues or a third dimension. In general, the trend is to recapitulate aspects of in vivo microenvironment into in vitro culture systems. The emphasis of my research has been on creating cell culture conditions for accumulation and retention of endogenous signals secreted by cells. Indeed, in vivo cells are lodged within gel-like matrix that limits diffusion of secreted signals whereas in a typical in vitro culture system cells reside in a large volume of media that serves as a sink for secreted signals. To remedy this, I have been developing small volume culture systems that create high cell number per volume ratio. My dissertation describes cultivation of primary hepatocytes in microfluidic channels under diffusion-controlled transport regime. Hepatocytes in such devices exhibited remarkable enhancement in phenotype and function, in large part due to accumulation of endogenous inductive signals including hepatocyte growth factor (HGF). Microfluidic systems are typically associated with small numbers of cells and relatively difficult to handle. Therefore, I developed low volume multi-well plates which recreated volume-induced phenotype enhancement in a format more amenable to scale-up and high throughput screening. In addition to hepatocytes, my dissertation highlights the utility of low volume cultures for long-term maintenance of primary bladder cancer cells. I demonstrate that these cells may be cultured in spheroid format inside microfluidic chambers for over 30 days without loss of viability or phenotype and that these cancer cells exhibit a pattern of drug responsiveness/resistance observed in vivo. Overall, my research has highlighted the importance of local volume and geometry in development of effective cell culture systems.

Microfluidics in Cell BiologyPart A Microfluidics for multicellular systems

Microfluidics in Cell BiologyPart A  Microfluidics for multicellular systems Book
Author : Anonim
Publisher : Academic Press
Release : 2018-07-20
ISBN : 0128142812
Language : En, Es, Fr & De

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Book Description :

Microfluidics in Cell Biology Part A: Volume 146, the latest release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are sections on Cell monolayers/spheroids, Collective migration in microtubes, Leukocyte adhesion dynamics on endothelial monolayers under flow, Constrained spheroid for perfusion culture, Cells in droplet arrays, Heart on chips, Kidney on chips, Liver on chips and hepatic immune responses, Gut on chips, 3D microvascular model-based lymphoma model, Blood brain barrier on chips, Multi-organ-on-a-chip for pharmacokinetic analysis, Cancer immunotherapy on chips, and more. Contains contributions from experts in the field from across the globe Covers a wide array of topics on both mitosis and meiosis Includes relevant, analysis based topics

Microfluidic Platforms for Cell Culture and Microenvironment Control

Microfluidic Platforms for Cell Culture and Microenvironment Control Book
Author : Yandong Gao
Publisher : Unknown
Release : 2011
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Microfluidic Platforms for Cell Culture and Microenvironment Control book written by Yandong Gao, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Microfluidics Based Microsystems

Microfluidics Based Microsystems Book
Author : S. Kakaç,B. Kosoy,D. Li,A. Pramuanjaroenkij
Publisher : Springer
Release : 2010-06-30
ISBN : 9048190290
Language : En, Es, Fr & De

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Book Description :

This volume contains an archival record of the NATO Advanced Study Institute on Microfluidics Based Microsystems – Fundamentals and App- cations held in Çe ?me-Izmir, Turkey, August 23–September 4, 2009. ASIs are intended to be high-level teaching activity in scientific and technical areas of current concern. In this volume, the reader may find interesting chapters and various microsystems fundamentals and applications. As the world becomes increasingly concerned with terrorism, early - spot detection of terrorist’s weapons, particularly bio-weapons agents such as bacteria and viruses are extremely important. NATO Public Diplomacy division, Science for Peace and Security section support research, Advanced Study Institutes and workshops related to security. Keeping this policy of NATO in mind, we made such a proposal on Microsystems for security. We are very happy that leading experts agreed to come and lecture in this important NATO ASI. We will see many examples that will show us Microfluidics usefulness for rapid diagnostics following a bioterrorism attack. For the applications in national security and anti-terrorism, microfluidic system technology must meet the challenges. To develop microsystems for security and to provide a comprehensive state-of-the-art assessment of the existing research and applications by treating the subject in considerable depth through lectures from eminent professionals in the field, through discussions and panel sessions are very beneficial for young scientists in the field.

Microfluidic Technologies for Human Health

Microfluidic Technologies for Human Health Book
Author : Utkan Demirci,Ali Khademhosseini,Robert Langer,Jeffrey Blander
Publisher : World Scientific
Release : 2012-12-26
ISBN : 9814405531
Language : En, Es, Fr & De

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Book Description :

The field of microfluidics has in the last decade permeated many disciplines, from physics to biology and chemistry, and from bioengineering to medical research. One of the most important applications of lab-on-a-chip devices in medicine and related disciplines is disease diagnostics, which involves steps from biological sample/analyte loading to storage, detection, and analysis. The chapters collected in this book detail recent advances in these processes using microfluidic devices and systems. The reviews of portable devices for diagnostic purposes are likely to evoke interest and raise new research questions in interdisciplinary fields (e.g., efficient MEMS/microfluidic engineering driven by biological and medical applications). The variety of the selected topics (general relevance of microfluidics in medical and bioengineering research, fabrication, advances in on-chip sample detection and analysis, and specific disease models) ensures that each of them can be viewed in the larger context of microfluidic-mediated diagnostics. Contents:A Microscale Bioinspired Cochlear-like Sensor (Robert D White, Robert Littrell, and Karl Grosh)Systematic Evaluation of the Efficiencies of Proteins and Chemicals in Pharmaceutical Applications (Morgan Hamon and Jong Wook Hong)Microfluidic Glucose Sensors (Jithesh V Veetil, Sruthi Ravindranathan, Sha Jin, and Kaiming Ye)Applications of Microfabrication and Microfluidic Techniques in Mesenchymal Stem Cell Research (Abhijit Majumder, Jyotsna Dhawan, Oren Levy, and Jeffrey M Karp)Patient-Specific Modeling of Low-Density Lipoprotein Transport in Coronary Arteries (Ufuk Olgac)Point-of-Care Microdevices for Global Health Diagnostics of Infectious Diseases (Sau Yin Chin, Tassaneewan Laksanasopin, Curtis D Chin, and Samuel K Sia)Integrated Microfluidic Sample Preparation for Chip-based Molecular Diagnostics (Jane Y Zhang, Qingqing Cao, Madhumita Mahalanabis, and Catherine Klapperich)Microfluidic Devices for Cellular Proteomic Studies (Yihong Zhan and Chang Lu)Microfluidics for Neuroscience: Novel Tools and Future Implications (Vivian M Hernandez and P Hande Özdinler)Microfluidics: On-Chip Platforms as In Vitro Disease Models (Shan Gao, Erkin Şeker, and Martin L Yarmush)Application of Microfluidics in Stem Cell and Tissue Engineering (Sasha H Bakhru, Christopher Highley, and Stefan Zappe)Microfluidic “On-the-Fly” Fabrication of Microstructures for Biomedical Applications (Edward Kang, Sau Fung Wong, and Sang-Hoon Lee)Microfluidics as a Promising Tool Toward Distributed Viral Detection (Elodie Sollier and Dino Di Carlo)Electrophoresis and Dielectrophoresis for Lab-on-a-Chip (LOC) Analyses (Yağmur Demircan, Gürkan Yilmaz, and Haluk Külah)Ultrasonic Embossing of Carbon Nanotubes for the Fabrication of Polymer Microfluidic Chips for DNA Sample Purification (Puttachat Khuntontong, Min Gong, and Zhiping Wang)Ferrofluidics (A Rezzan Kose and Hur Koser)Antibody-based Blood Bioparticle Capture and Separation Using Microfluidics for Global Health (ZhengYuan Luo, ShuQi Wang, Utkan Demirci, TianJian Lu, Feng Xu, and BoFeng Bai)Applications of Quantum Dots for Fluorescence Imaging in Biomedical Research (ShuQi Wang, Matin Esfahani, Dusan Sarenac, Bettina Cheung, Aishwarya Vasudevan, Fatih Inci, and Utkan Demirci) Readership: Engineers, academic researchers and instructors, and industry researchers involved in microfluidic technologies. Keywords:Point-of-Care;Rapid Detection and Monitoring;Infectious Diseases;Biomedical Engineering / Bioengineering;Stem Cells Research;Biotechnology;Tissue Engineering

Nanobiomaterials

Nanobiomaterials Book
Author : Dong Yi,Georgia C. Papaefthymiou
Publisher : CRC Press
Release : 2013-08-14
ISBN : 1439876428
Language : En, Es, Fr & De

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Book Description :

There is a continuous exchange of ideas taking place at the border of the biological and physical sciences in many areas of nanoscience. Nanotechnology uses biomimetic or bio-inspired processes to produce nanosized materials for applications in biology and other fields. In return, the fruits of nanotechnology are applied to expanding areas of biome