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Microbial Cell Factories

Microbial Cell Factories Book
Author : Deepansh Sharma,Baljeet Singh Saharan
Publisher : CRC Press
Release : 2018-03-22
ISBN : 1351668056
Language : En, Es, Fr & De

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Book Description :

Microbial Cell Factories is a conceptual, reference-based source including chapters covering microbial cell factories for industrial developments, microbial biotechnology, sustainable environmental solutions, agriculture practices, microorganisms in food processing, metabolites as next generation food additives/food processing, and microbial cell factories in alternative energy fuel generation. The book highlights trends and developments in the field of microbial products, written by an international team of leading academic and research scholars. Key Selling Features: Highlights trends and developments in microbial biotechnology Systematically reviews microbial cell factories Explores the potential of microbial cell derived industrial production Synthesizes information on environmental and agricultural uses of microbial biotechnology Contributions from an international team of leading scholars

DNA Replication Control in Microbial Cell Factories

DNA Replication Control in Microbial Cell Factories Book
Author : Monika Glinkowska,Lidia Boss,Grzegorz Wegrzyn
Publisher : Springer
Release : 2014-09-23
ISBN : 3319105337
Language : En, Es, Fr & De

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Book Description :

This work describes the current knowledge of biochemical mechanisms regulating initiation of DNA replication in Escherichia coli, which focuses on the control of activity of the DnaA protein. Examples of direct linkages between DNA replication and other cellular processes are provided. In addition, similarities of the mechanisms of regulation of DNA replication operating in prokaryotic and eukaryotic cells are identified, and implications for understanding more complex processes, like carcinogenesis are suggested. Studies of recent years provided evidence that regulation of DNA replication in bacteria is more complex than previously anticipated. Multiple layers of control seem to ensure coordination of this process with the increase of cellular mass and the division cycle. Metabolic processes and membrane composition may serve as points where integration of genome replication with growth conditions occurs. It is also likely that coupling of DNA synthesis with cellular metabolism may involve interactions of replication proteins with other macromolecular complexes, responsible for various cellular processes. Thus, the exact set of factors participating in triggering the replication initiation may differ depending on growth conditions. Therefore, understanding the regulation of DNA duplication requires placing this process in the context of the current knowledge on bacterial metabolism, as well as cellular and chromosomal structure. Moreover, in both Escherichia coli and eukaryotic cells, replication initiator proteins were shown to play other roles in addition to driving the assembly of replication complexes, which constitutes another, yet not sufficiently understood, layer of coordinating DNA replication with the cell cycle.

Microbial Cell Factories

Microbial Cell Factories Book
Author : Deepansh Sharma,Baljeet Singh Saharan
Publisher : CRC Press
Release : 2021-03-30
ISBN : 9780367657307
Language : En, Es, Fr & De

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Book Description :

Microbial Cell Factories is a conceptual, reference-based source including chapters covering microbial cell factories for industrial developments, microbial biotechnology, sustainable environmental solutions, agriculture practices, microorganisms in food processing, metabolites as next generation food additives/food processing, and microbial cell factories in alternative energy fuel generation. The book highlights trends and developments in the field of microbial products, written by an international team of leading academic and research scholars. Key Selling Features: Highlights trends and developments in microbial biotechnology Systematically reviews microbial cell factories Explores the potential of microbial cell derived industrial production Synthesizes information on environmental and agricultural uses of microbial biotechnology Contributions from an international team of leading scholars

Systems Metabolic Engineering of Microbial Cell Factories for the Synthesis of Value added Chemicals

Systems Metabolic Engineering of Microbial Cell Factories for the Synthesis of Value added Chemicals Book
Author : Arul Mozhy Varman
Publisher : Unknown
Release : 2013
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Microbial cell factories offer us an excellent opportunity for the conversion of many different cheaply available raw materials into valuable chemicals. Systems metabolic engineering aims at developing rational strategies for the engineering of microbial hosts by providing global level information of a cell. This dissertation focuses on metabolic engineering, bioprocess modeling and pathway analysis, to develop robust microbial cell factories for the synthesis of value-added chemicals. The following research tasks were completed in this regard. First, statistical models were developed for the prediction of product yields in engineered microbial cell factories - cerevisiae and Escherichia coli (Chapter 2). A large space of experimental data for chemical production from recent references was collected and a statistics-based model was developed to calculate production yield. The input variables (numerical or categorical variables) for the model represented the number of enzymatic steps in the biosynthetic pathway of interest, metabolic modifications, cultivation modes, nutrition and oxygen availability. In addition, the use of 13C-isotopomer analysis method was proposed for the accurate determination of product yields in engineered microbes under complex cultivation conditions (Chapter 3). Second, metabolic engineering of the cyanobacterium, Synechocystis sp. PCC 6803 was performed for synthesizing isobutanol under phototrophic conditions (Chapter 4). With the expression of the heterologous genes from the Ehrlich Pathway, by incorporating an in situ isobutanol harvesting system, and also by employing mixotrophic conditions, the engineered Synechocystis 6803 strain accumulated a maximum of ~300 mg/L of isobutanol in a 21 day culture. In addition, Synechocystis 6803 was engineered for the synthesis of D-lactic acid (Chapter 5), via overexpression of a novel D-lactate dehydrogenase (encoded by gldA101). The production of D-lactate was further improved by employing three strategies: (i) cofactor balancing, (ii) codon optimization, and (iii) process optimization. The engineered Synechocystis 6803 produced 2.2 g/L D-lactate under photoautotrophic conditions with acetate, the highest reported lactate titer among all known cyanobacterial strains. Finally, an E. coli cell factory was engineered to study the fermentation kinetics for scaled-up isobutanol production (Chapter 6). Through kinetic modeling (to describe the dynamics of biomass, products and glucose concentration) and isotopomer analysis, we have also offered metabolic insights into the performance trade-off between two engineered isobutanol producing E. coli strains (a high performance and a low performance strain). The kinetic model can also predict isobutanol production under different fermentation conditions. I and my colleagues have also demonstrated that E. coli cell factory can also be used for converting waste acetate into free fatty acids through metabolic engineering. In conclusion, the opportunities and commercial limitations with current biotechnology as well as the role of systems metabolic engineering for the development of high performance microbial cell factories were discussed (Chapter 7).

Microbial Cell Factories Engineering for Production of Biomolecules

Microbial Cell Factories Engineering for Production of Biomolecules Book
Author : Vijai Singh
Publisher : Academic Press
Release : 2021-02-26
ISBN : 0128214783
Language : En, Es, Fr & De

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Book Description :

Microbial Cell Factories Engineering for Production of Biomolecules presents a compilation of chapters written by eminent scientists worldwide. Sections cover major tools and technologies for DNA synthesis, design of biosynthetic pathways, synthetic biology tools, biosensors, cell-free systems, computer-aided design, OMICS tools, CRISPR/Cas systems, and many more. Although it is not easy to find relevant information collated in a single volume, the book covers the production of a wide range of biomolecules from several MCFs, including Escherichia coli, Bacillus subtilis, Pseudomonas putida, Streptomyces, Corynebacterium, Cyanobacteria, Saccharomyces cerevisiae, Pichia pastoris and Yarrowia lipolytica, and algae, among many others. This will be an excellent platform from which scientific knowledge can grow and widen in MCF engineering research for the production of biomolecules. Needless to say, the book is a valuable source of information not only for researchers designing cell factories, but also for students, metabolic engineers, synthetic biologists, genome engineers, industrialists, stakeholders and policymakers interested in harnessing the potential of MCFs in several fields. Offers basic understanding and a clear picture of various MCFs Explains several tools and technologies, including DNA synthesis, synthetic biology tools, genome editing, biosensors, computer-aided design, and OMICS tools, among others Harnesses the potential of engineered MCFs to produce a wide range of biomolecules for industrial, therapeutic, pharmaceutical, nutraceutical and biotechnological applications Highlights the advances, challenges, and future opportunities in designing MCFs

Population dynamics in microbial cell factories

Population dynamics in microbial cell factories Book
Author : Anonim
Publisher : Unknown
Release : 2022-01-22
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Population dynamics in microbial cell factories book written by , available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Metabolomics Investigation of Microbial Cell Factories

Metabolomics Investigation of Microbial Cell Factories Book
Author : Howbeer Muhamad Ali
Publisher : Unknown
Release : 2015
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Metabolomics Investigation of Microbial Cell Factories book written by Howbeer Muhamad Ali, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Optimization of Microbial Cell Factories with Systems Biology

Optimization of Microbial Cell Factories with Systems Biology Book
Author : Zachary Andrew King
Publisher : Unknown
Release : 2016
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Microbial cell factories can have a transformative impact the chemical industry, but, first, we must meet the challenges of designing and optimizing high-yield cell factory strains. The most popular conceptual model for cell factory optimization is the design-build-test-learn cycle. I present methods that use systems biology to improve the optimization process in each of these steps. First, the build step requires a parts list for a host organism and any heterologous pathways. I present BiGG Models, a database of more than 75 high-quality, manually-curated genome-scale metabolic models that comprise a standardized metabolic parts list. BiGG Models has become the most popular resource in the community for gold-standard genome-scale metabolic models. For the test step, contextualization of omics data is an enormous challenge, and I developed a visualization tool to address this challenge. Escher is a web application for visualizing data on biological pathways. With Escher, users can identify trends in common genomic data types (e.g. RNA-Seq, proteomics, ChIP) and metabolite- and reaction-oriented data types (e.g. metabolomics, fluxomics). For the learn step, genome-scale models can be used to identify general trends in cell factories performance. I introduce a computational method--OptSwap--to predict bioprocessing strain designs by identifying optimal modifications of the cofactor binding specificities of oxidoreductase enzyme and identifying complementary reaction knockouts. I also present an optimization procedure that identifies optimal cofactor- specificity "swaps" for improving theoretical yield in genome-scale metabolic models. Swapping the cofactor specificity of central metabolic enzymes is shown to increase NADPH production and increase theoretical yields for many native and non-native products. Last, the design step requires models that can successfully predict phenotype from genotype. I assess the predictive capabilities of existing models of E. coli through literature mining. I simulate strains from the literature in six historical genome-scale models of E. coli and report that the predictive power of the models has increased as they have expanded in size and scope. Together, these studies provide a path toward successfully applying systems biology methods to optimizing microbial cell factories.

Analysis Design and Synthesis of Microbial Cell Factories Producing N butanol

Analysis  Design and Synthesis of Microbial Cell Factories Producing N butanol Book
Author : Kirsten Anna Katrin Köhler
Publisher : Unknown
Release : 2014
ISBN : 9783844030525
Language : En, Es, Fr & De

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Book Description :

Download Analysis Design and Synthesis of Microbial Cell Factories Producing N butanol book written by Kirsten Anna Katrin Köhler, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Biotechnology of Terpenoid Production from Microbial Cell Factories

Biotechnology of Terpenoid Production from Microbial Cell Factories Book
Author : Pratima Gupta,Suresh Phulara
Publisher : Academic Press
Release : 2021-04-17
ISBN : 0128231785
Language : En, Es, Fr & De

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Book Description :

Biotechnology of Terpenoid Production from Microbial Cell Factories is a unique reference to help researchers, scientists and scholars explore available strategies involved in the production of terpenoid-based, value-added compounds from GRAS status (Generally Recognized as Safe by FDA) microbes to address the associated challenges for the industry. The book covers the most up-to-date information about microbial terpenoid production, including culture condition modulation for the improved and high-specificity production of terpenoid and their in-situ extraction. Each class of terpenoid is explained in detail, including their nutritional and pharmaceutical information and their molecular aspects. Provides an in-depth look at both the natural and metabolic engineering aspects of microbial terpenoid production Includes production strategies and existing challenges for each class of terpenoid from microbial sources Presents broad application opportunities of terpenoids such as food additives, pharmaceuticals and fragrances Includes brief chapter summaries and a glossary for each chapter to simplify technical applications

Current Developments in Biotechnology and Bioengineering

Current Developments in Biotechnology and Bioengineering Book
Author : Ashok Pandey,Swati Joshi,Ranjna Sirohi,Sung Hoon Park
Publisher : Elsevier
Release : 2022-07-15
ISBN : 9780323885041
Language : En, Es, Fr & De

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Book Description :

Designer Microbial Cell Factories: Metabolic Engineering and Applications, the latest release in the Current Developments in Biotechnology and Bioengineering series, provides a detailed overview of the biotechnological approaches and strategies used to generate engineered microbes and to facilitate the acceleration, modulation and diversion of metabolic pathways to get desired output such as production of value-added compound or biodegradation of xenobiotic pollutant. The book also highlights applied aspects of designer microbes in fields as diverse as agriculture, pharmaceuticals and bioremediation. Designer microbes generated through reprogramming the microbial cell factories (MCFs) provide an edge over their natural counterparts in terms of increased molecular diversity and selective chemistry. These bugs are becoming instrumental in several areas, including agriculture, environment and human health. Engineering microbes through directed evolution not only gives freedom from evolutionary constrains but also allow introduction of regulated and foreseeable functions into MCFs. Dedicated to the designing of microbes, covering state-of-the-art technological advancements in the field Includes applications of metabolic engineering in the field of agriculture, bioremediation, value-added products, therapeutics, and more Contains chapters dedicated to innovative approaches surrounding engineered microbial consortia Provides comprehensive details and helps users understand concepts

Systems Metabolic Engineering

Systems Metabolic Engineering Book
Author : Christoph Wittmann,Sang Yup Lee
Publisher : Springer Science & Business Media
Release : 2012-06-15
ISBN : 9400745346
Language : En, Es, Fr & De

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Book Description :

Systems Metabolic Engineering is changing the way microbial cell factories are designed and optimized for industrial production. Integrating systems biology and biotechnology with new concepts from synthetic biology enables the global analysis and engineering of microorganisms and bioprocesses at super efficiency and versatility otherwise not accessible. Without doubt, systems metabolic engineering is a major driver towards bio-based production of chemicals, materials and fuels from renewables and thus one of the core technologies of global green growth. In this book, Christoph Wittmann and Sang-Yup Lee have assembled the world leaders on systems metabolic engineering and cover the full story – from genomes and networks via discovery and design to industrial implementation practises. This book is a comprehensive resource for students and researchers from academia and industry interested in systems metabolic engineering. It provides us with the fundaments to targeted engineering of microbial cells for sustainable bio-production and stimulates those who are interested to enter this exiting research field.

Revelation of Yin Yang Balance in Microbial Cell Factories by Data Mining Flux Modeling and Metabolic Engineering

Revelation of  Yin Yang  Balance in Microbial Cell Factories by Data Mining  Flux Modeling  and Metabolic Engineering Book
Author : Gang Wu
Publisher : Unknown
Release : 2016
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

The long-held assumption of never-ending rapid growth in biotechnology and especially in synthetic biology has been recently questioned, due to lack of substantial return of investment. One of the main reasons for failures in synthetic biology and metabolic engineering is the metabolic burdens that result in resource losses. Metabolic burden is defined as the portion of a host cell's resources -- either energy molecules (e.g., NADH, NADPH and ATP) or carbon building blocks (e.g., amino acids) -- that is used to maintain the engineered components (e.g., pathways). As a result, the effectiveness of synthetic biology tools heavily dependents on cell capability to carry on the metabolic burden. Although genetic modifications can effectively engineer cells and redirect carbon fluxes toward diverse products, insufficient cell ATP powerhouse is limited to support diverse microbial activities including product synthesis. Here, I employ an ancient Chinese philosophy (Yin-Yang) to describe two contrary forces that are interconnected and interdependent, where Yin represents energy metabolism in the form of ATP, and Yang represents carbon metabolism. To decipher "Yin-Yang" balance and its implication to microbial cell factories, this dissertation applied metabolic engineering, flux analysis, data mining tools to reveal cell physiological responses under different genetic and environmental conditions. Firstly, a combined approach of FBA and 13C-MFA was employed to investigate several engineered isobutanol-producing strains and examine their carbon and energy metabolism. The result indicated isobutanol overproduction strongly competed for biomass building blocks and thus the addition of nutrients (yeast extract) to support cell growth is essential for high yield of isobutanol. Based on the analysis of isobutanol production, 'Yin-Yang' theory has been proposed to illustrate the importance of carbon and energy balance in engineered strains. The effects of metabolic burden and respiration efficiency (P/O ratio) on biofuel product were determined by FBA simulation. The discovery of energy cliff explained failures in bioprocess scale-ups. The simulation also predicted that fatty acid production is more sensitive to P/O ratio change than alcohol production. Based on that prediction, fatty acid producing strains have been engineered with the insertion of Vitreoscilla hemoglobin (VHb), to overcome the intracellular energy limitation by improving its oxygen uptake and respiration efficiency. The result confirmed our hypothesis and different level of trade-off between the burden and the benefit from various introduced genetic components. On the other side, a series of computational tools have been developed to accelerate the application of fluxomics research. Microbesflux has been rebuilt, upgraded, and moved to a commercial server. A platform for fluxomics study as well as an open source 13C-MFA tool (WUFlux) has been developed. Further, a computational platform that integrates machine learning, logic programming, and constrained programming together has been developed. This platform gives fast predictions of microbial central metabolism with decent accuracy. Lastly, a framework has been built to integrate Big Data technology and text mining to interpret concepts and technology trends based on the literature survey. Case studies have been performed, and informative results have been obtained through this Big Data framework within five minutes. In summary, 13C-MFA and flux balance analysis are only tools to quantify cell energy and carbon metabolism (i.e., Yin-Yang Balance), leading to the rational design of robust high-producing microbial cell factories. Developing advanced computational tools will facilitate the application of fluxomics research and literature analysis.

Evaluating and Deploying Genome scale Metabolic Models for Microbial Cell Factories

Evaluating and Deploying Genome scale Metabolic Models for Microbial Cell Factories Book
Author : Anonim
Publisher : Unknown
Release : 2021
ISBN : 9789463956550
Language : En, Es, Fr & De

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Book Description :

Chapter 1 describes two paradigms: reductionism and holism in life sciences, Systems biology, genome-scale constraint-based metabolic models, Synthetic biology and the design build test learn cycle. Chapter 1 provides the background for all other chapters.

Metabolomics

Metabolomics Book
Author : Ute Roessner
Publisher : IntechOpen
Release : 2012-02-10
ISBN : 9789535100461
Language : En, Es, Fr & De

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Book Description :

Metabolomics is a rapidly emerging field in life sciences, which aims to identify and quantify metabolites in a biological system. Analytical chemistry is combined with sophisticated informatics and statistics tools to determine and understand metabolic changes upon genetic or environmental perturbations. Together with other 'omics analyses, such as genomics and proteomics, metabolomics plays an important role in functional genomics and systems biology studies in any biological science. This book will provide the reader with summaries of the state-of-the-art of technologies and methodologies, especially in the data analysis and interpretation approaches, as well as give insights into exciting applications of metabolomics in human health studies, safety assessments, and plant and microbial research.

Systems Metabolic Engineering

Systems Metabolic Engineering Book
Author : Christoph Wittmann,Sang Yup Lee
Publisher : Springer
Release : 2012-06-15
ISBN : 9789400745353
Language : En, Es, Fr & De

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Book Description :

Systems Metabolic Engineering is changing the way microbial cell factories are designed and optimized for industrial production. Integrating systems biology and biotechnology with new concepts from synthetic biology enables the global analysis and engineering of microorganisms and bioprocesses at super efficiency and versatility otherwise not accessible. Without doubt, systems metabolic engineering is a major driver towards bio-based production of chemicals, materials and fuels from renewables and thus one of the core technologies of global green growth. In this book, Christoph Wittmann and Sang-Yup Lee have assembled the world leaders on systems metabolic engineering and cover the full story – from genomes and networks via discovery and design to industrial implementation practises. This book is a comprehensive resource for students and researchers from academia and industry interested in systems metabolic engineering. It provides us with the fundaments to targeted engineering of microbial cells for sustainable bio-production and stimulates those who are interested to enter this exiting research field.

Transforming Lactococcus Lactis Into a Microbial Cell Factory

Transforming Lactococcus Lactis Into a Microbial Cell Factory Book
Author : Kia Vest Petersen
Publisher : Unknown
Release : 2014
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Transforming Lactococcus Lactis Into a Microbial Cell Factory book written by Kia Vest Petersen, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Fermentation Microbiology and Biotechnology Fourth Edition

Fermentation Microbiology and Biotechnology  Fourth Edition Book
Author : E. M. T. El-Mansi,Jens Nielsen,David Mousdale,Ross P. Carlson
Publisher : CRC Press
Release : 2018-12-17
ISBN : 0429015968
Language : En, Es, Fr & De

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Book Description :

Fermentation Microbiology and Biotechnology, 4th Edition explores and illustrates the broad array of metabolic pathways employed for the production of primary and secondary metabolites, as well as biopharmaceuticals. This updated and expanded edition addresses the whole spectrum of fermentation biotechnology, from fermentation kinetics and dynamics to protein and co-factor engineering. It also sheds light on the new strategies employed by industrialist for increasing tolerance and endurance of microorganisms to the accumulation of toxic wastes in microbial-cell factories. The new edition builds upon the fine pedigree of its earlier predecessors and extends the spectrum of the book to reflect the multidisciplinary and buoyant nature of this subject area. Key Features Covers the whole spectrum of the field from fermentation kinetics to control of fermentation and protein engineering. Includes case studies specifically designed to illustrate industrial applications and current state-of-the-art technologies. Presents the contributions of eminent international academics and industrial experts. Offers new chapters addressing: The prospects and the role of bio-fuels refineries, Control of metabolic efflux to product formation in microbial-cell factories and Improving tolerance of microorganisms to toxic byproduct accumulation in the fermentation vessel.

Production of a Functional Human Milk Oligosaccharide 2 fucosyllactose Using Microbial Cell Factories

Production of a Functional Human Milk Oligosaccharide  2  fucosyllactose  Using Microbial Cell Factories Book
Author : Panchalee Pathanibul
Publisher : Unknown
Release : 2015
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Production of a Functional Human Milk Oligosaccharide 2 fucosyllactose Using Microbial Cell Factories book written by Panchalee Pathanibul, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Pseudomonas Putida as a Microbial Cell Factory

Pseudomonas Putida as a Microbial Cell Factory Book
Author : Vinoth Wigneswaran
Publisher : Unknown
Release : 2016
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Pseudomonas Putida as a Microbial Cell Factory book written by Vinoth Wigneswaran, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.