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The Metabolic Pathway Engineering Handbook Two Volume Set

The Metabolic Pathway Engineering Handbook  Two Volume Set Book
Author : Christina Smolke
Publisher : CRC Press
Release : 2009-08-18
ISBN : 1420005626
Language : En, Es, Fr & De

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Book Description :

Christina Smolke, who recently developed a novel way to churn out large quantities of drugs from genetically modified brewer's yeast, is regarded as one of the most brilliant minds in biomedical engineering. In this handbook, she brings together pioneering scientists from dozens of disciplines to provide a complete record of accomplishment in metab

Metabolic Pathway Design

Metabolic Pathway Design Book
Author : Pablo Carbonell
Publisher : Springer Nature
Release : 2019-11-05
ISBN : 3030298655
Language : En, Es, Fr & De

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Book Description :

This textbook presents solid tools for in silico engineering biology, offering students a step-by-step guide to mastering the smart design of metabolic pathways. The first part explains the Design-Build-Test-Learn-cycle engineering approach to biology, discussing the basic tools to model biological and chemistry-based systems. Using these basic tools, the second part focuses on various computational protocols for metabolic pathway design, from enzyme selection to pathway discovery and enumeration. In the context of industrial biotechnology, the final part helps readers understand the challenges of scaling up and optimisation. By working with the free programming language Scientific Python, this book provides easily accessible tools for studying and learning the principles of modern in silico metabolic pathway design. Intended for advanced undergraduates and master’s students in biotechnology, biomedical engineering, bioinformatics and systems biology students, the introductory sections make it also useful for beginners wanting to learn the basics of scientific coding and find real-world, hands-on examples.

The Metabolic Pathway Engineering Handbook

The Metabolic Pathway Engineering Handbook Book
Author : Christina Smolke
Publisher : CRC Press
Release : 2009-07-28
ISBN : 9781439802977
Language : En, Es, Fr & De

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Book Description :

This first volume of the Metabolic Pathway Engineering Handbook provides an overview of metabolic pathway engineering with a look towards the future. It discusses cellular metabolism, including transport processes inside the cell and energy generating reactions, as well as rare metabolic conversions. This volume also explores balances and reaction

Metabolic Pathway Engineering

Metabolic Pathway Engineering Book
Author : Jean F. Challacombe
Publisher : CRC Press
Release : 2021-07-15
ISBN : 1000260542
Language : En, Es, Fr & De

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Book Description :

Metabolic systems engineering combines the tools and approaches of systems biology, synthetic biology, and evolutionary engineering. This book reviews studies on metabolism, from the earliest work of Lavoisier and Buchner to current cutting-edge research in metabolic systems engineering. This technology has been used in bioengineering applications to create high-performing microbes and plants that produce important chemicals, pharmaceuticals, crops, and other natural products. Current applications include optimizing metabolic pathways to enhance degradation of biomass for biofuel production and accelerated processing of environmental waste products and contaminants. The book includes examples to illustrate the applications of this technology in the optimization of metabolic pathways to create robust industrial strains as well as in the engineering of biological processes involving health and diseases of humans, animals, and plants. Written by a seasoned computational biologist with many years of experience in genomics, bioinformatics, and systems biology, this book will appeal to anyone interested in metabolic systems analysis and metabolic pathway engineering.

Metabolic Pathway Engineering

Metabolic Pathway Engineering Book
Author : Michael E. Himmel,Yannick J. Bomble
Publisher : Humana
Release : 2021-08-11
ISBN : 9781071601976
Language : En, Es, Fr & De

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Book Description :

This book illustrates experimental and computational methodologies used to achieve cost effective biological processes for the production of fuels and biochemicals through multiple approaches to increasing yield, titers, and productivity in a robust host. The volume includes the most recent and cutting-edge aspects of pathway engineering, flux analysis, and metabolic enzyme engineering. Each chapter highlights the complexity and challenges of the problem as well as the methods used to solve this problem or changes needed in current methods. As a part of the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice that gives researchers a much needed boost. Authoritative and practical, Metabolic Pathway Engineering benefits not only scientists working on more fundamental aspects of this endeavor but also those in the biochemical industry working on strain engineering for robust industrial processes.

The Metabolic Pathway Engineering Handbook

The Metabolic Pathway Engineering Handbook Book
Author : Anonim
Publisher : Unknown
Release : 2010
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download The Metabolic Pathway Engineering Handbook book written by , available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Pathway Analysis and Optimization in Metabolic Engineering

Pathway Analysis and Optimization in Metabolic Engineering Book
Author : Néstor V. Torres,Eberhard O. Voit
Publisher : Cambridge University Press
Release : 2002-12-19
ISBN : 9781139437615
Language : En, Es, Fr & De

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Book Description :

Facility in the targeted manipulation of the genetic and metabolic composition of organisms, combined with unprecedented computational power, is forging a niche for a new subspecialty of biotechnology called metabolic engineering. First published in 2002, this book introduces researchers and advanced students in biology and engineering to methods of optimizing biochemical systems of biotechnological relevance. It examines the development of strategies for manipulating metabolic pathways, demonstrates the need for effective systems models, and discusses their design and analysis, while placing special emphasis on optimization. The authors propose power-law models and methods of biochemical systems theory toward these ends. All concepts are derived from first principles, and the text is richly illustrated with numerous graphs and examples throughout. Special features include: nontechnical and technical introductions to models of biochemical systems; a review of basic methods of model design and analysis; concepts of optimization; and detailed case studies.

A Synthetic Biology Approach to Metabolic Pathway Engineering

A Synthetic Biology Approach to Metabolic Pathway Engineering Book
Author : Christine Merrick
Publisher : Unknown
Release : 2015
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download A Synthetic Biology Approach to Metabolic Pathway Engineering book written by Christine Merrick, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Metabolic Pathway Engineering of the Toluene Degradation Pathway

Metabolic Pathway Engineering of the Toluene Degradation Pathway Book
Author : Lucy Regan
Publisher : Unknown
Release : 1995
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Metabolic Pathway Engineering of the Toluene Degradation Pathway book written by Lucy Regan, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Metabolic Engineering

Metabolic Engineering Book
Author : G. Stephanopoulos,Aristos A. Aristidou,Jens Høiriis Nielsen,Jens Nielsen
Publisher : Unknown
Release : 1998
ISBN : 9780126662603
Language : En, Es, Fr & De

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Book Description :

Metabolic engineering is a new field with applications in the production of chemicals, fuels, materials, pharmaceuticals, and medicine at the genetic level. The field's novelty is in the synthesis of molecular biology techniques and the tools of mathematical analysis, which allow rational selection of targets for genetic modification through measurements and control of metabolic fluxes. The objective is to identify specific genetics or environmental manipulations that result in improvements in yield and productivities of biotechnological processes. Key features of the book are pathway integration and the focus on metabolic flux as a fundamental determinant of cell physiology. The book keeps mathematical complexity to a minimum, and provides a glossary of biological terms to facilitate use of the book by a broader spectrum of readers. A web page exists to communicate updates of the codes and homework problems. Key Features * Demonstrates metabolic engineering in action with numerous examples of pathway modification * Includes methods for identifying key enzymes in metabolic networks * Contains a comprehensive review of metabolic biochemistry * Discusses metabolic regulation at the gene, enzyme, operon, and cell levels * Explains concepts of stoichiometry, kinetics, and thermodynamics of metabolic pathways * Minimizes mathematical complexity * Links to a Web page to communicate updates of the software code and homework problems

Metabolic Engineering

Metabolic Engineering Book
Author : Jens Høiriis Nielsen,Jens Nielsen,L. Eggeling
Publisher : Springer Science & Business Media
Release : 2001
ISBN : 9783540418481
Language : En, Es, Fr & De

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Book Description :

Metabolic engineering is a rapidly evolving field that is being applied for the optimization of many different industrial processes. In this issue of Advances in Biochemical Engineering/Biotechnology, developments in different areas of metabolic engineering are reviewed. The contributions discuss the application of metabolic engineering in the improvement of yield and productivity - illustrated by amino acid production and the production of novel compounds - in the production of polyketides and extension of the substrate range - and in the engineering of S. cerevisiae for xylose metabolism, and the improvement of a complex biotransformation process.

Metabolic Pathway Engineering and Compartmentalization Strategies in Yeast for Biotechnological Purposes

Metabolic Pathway Engineering and Compartmentalization Strategies in Yeast for Biotechnological Purposes Book
Author : Mara Elisa Reifenrath
Publisher : Unknown
Release : 2018
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Metabolic Pathway Engineering and Compartmentalization Strategies in Yeast for Biotechnological Purposes book written by Mara Elisa Reifenrath, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

23 European Symposium on Computer Aided Process Engineering

23 European Symposium on Computer Aided Process Engineering Book
Author : Carlos A.M. Riascos,Andreas K. Gombert,Luiziane F. Silva,Marilda K. Taciro,José G.C. Gomez,Galo A.C. Le Roux
Publisher : Elsevier Inc. Chapters
Release : 2013-06-10
ISBN : 0128085193
Language : En, Es, Fr & De

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Book Description :

Metabolic flux analysis is a useful tool for metabolism characterization and verification of genetic modification effects; it is a support for decisions on biotechnological process improvement. Commercial production of biodegradable polymers, specifically polyhydroxyalkanoates PHAs, is restricted by production costs, which may be cut by increasing yield from substrate to product, since carbon source for PHAs production accounts up to 50% of the total production costs; additionally, in Pseudomonas sp. LMF046 the experimental yield is between 60-70% of theoretical maximum yield. This work presents metabolic pathways identification, flux quantification and analysis on this strain, employing position isotopomer balancing and measurements of labeling patterns in the polymer by GC-MS. Initial results –using [1-13C]glucose-allow to rule out carbohydrate catabolism by EMP pathway, whereas final ones –using a mixture of [U-13C]glucose and natural glucose– allow to estimate fraction of glucose metabolized by ED and PP pathways. Metabolic network includes eight intracellular metabolite and 324 isotopomer balances, and it is solved in 1.3 seconds in a Core i5 PC. Sensitivity analysis shows inclusion of carbon natural labeling improves the prediction. The estimated ratio for sugar metabolism into PP and ED pathways, 1.35:0.55, that corresponds to 77% of the theoretical yield, leads to the conclusion that the glucose metabolism in larger proportion by the Pentoses pathway is the main reason for a low yield. The problem has been solved satisfactorily, and a sensitivity analysis shows that it is necessary to reduce uncertainty on labeling measurements.

Metabolic Engineering

Metabolic Engineering Book
Author : Prerna Pandey
Publisher : Delve Publishing
Release : 2017-11
ISBN : 9781773611488
Language : En, Es, Fr & De

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Book Description :

Metabolic engineering is the process of modifying biochemical networks and regulatory processes within cells to improve the production of a desired substances. The process involves analyzing and engineering metabolic pathways by the use of experimental and modeling techniques, as well as by several genome-wide analysis techniques. Briefly, the process involves identifying a metabolic pathway for obtaining the desired productivity, mathematical analysis of the pathway to find the theoretical yield, followed by genetic manipulations of those reactions to maximize the yield, and finally, experimental measurements and fine tuning of the pathways.The information compiled in this book will help the readers have a better knowledge of the basic process of metabolic engineering, the latest developments and its applications in plants and microorganisms for overproduction and isolation of high-value chemicals. We have also included case studies for a better understanding of the concept. We look forward to feedback/suggestions on this humble attempt.

Metabolic Engineering

Metabolic Engineering Book
Author : Sang Yup Lee,Jens Nielsen,Gregory Stephanopoulos
Publisher : John Wiley & Sons
Release : 2021-06-02
ISBN : 352782345X
Language : En, Es, Fr & De

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Book Description :

Learn more about foundational and advanced topics in metabolic engineering in this comprehensive resource edited by leaders in the field Metabolic Engineering: Concepts and Applications delivers a one-stop resource for readers seeking a complete description of the concepts, models, and applications of metabolic engineering. This guide offers practical insights into the metabolic engineering of major cell lines, including E. Coli, Bacillus and Yarrowia Lipolytica, and organisms, including human, animal, and plant). The distinguished editors also offer readers resources on microbiome engineering and the use of metabolic engineering in bioremediation. Written in two parts, Metabolic Engineering begins with the essential models and strategies of the field, like Flux Balance Analysis, Quantitative Flux Analysis, and Proteome Constrained Models. It also provides an overview of topics like Pathway Design, Metabolomics, and Genome Editing of Bacteria and Eukarya. The second part contains insightful descriptions of the practical applications of metabolic engineering, including specific examples that shed light on the topics within. In addition to subjects like the metabolic engineering of animals, humans, and plants, you’ll learn more about: Metabolic engineering concepts and a historical perspective on their development The different modes of analysis, including flux balance analysis and quantitative flux analysis An illuminating and complete discussion of the thermodynamics of metabolic pathways The Genome architecture of E. coli, as well as genome editing of both bacteria and eukarya An in-depth treatment of the application of metabolic engineering techniques to organisms including corynebacterial, bacillus, and pseudomonas, and more Perfect for students of biotechnology, bioengineers, and biotechnologists, Metabolic Engineering: Concepts and Applications also has a place on the bookshelves of research institutes, biotechnological institutes and industry labs, and university libraries. It's comprehensive treatment of all relevant metabolic engineering concepts, models, and applications will be of use to practicing biotechnologists and bioengineers who wish to solidify their understanding of the field.

Computational Design and Optimization of Metabolic Pathways

Computational Design and Optimization of Metabolic Pathways Book
Author : Chiam Ng
Publisher : Unknown
Release : 2017
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Microbial production of chemicals and fuels has often been cited as the highly viable solution to the current energy crisis. It has also been widely exploited to produce value-added products such as pharmaceuticals, nutraceuticals, food flavorings and fine chemicals. Knowledge gained from genome sequencing and functional annotations, as well as advances in genetic engineering techniques, enable us to re-code the genetic blueprint of a microbial cell to endow them with new functions such as a non-native metabolic pathway that make a specific biochemical. As the cells are often not optimized to synthesize the target product, significant rewiring of their metabolic networks is required to re-apportion carbon flux towards the target product. This should be performed with careful consideration of cellular protein resource allocation as well as energy and redox balance. However, the task is often complicated by the intricate interplay of the cellular gene-protein-reaction network. In this dissertation, we aim to address several challenges in designing and engineering metabolic pathways by combining techniques in computational biology, metabolic engineering, and synthetic biology. Each project relies extensively on optimization-based computational design, from genetic constructs to pathways and finally the entire metabolic network, to achieve the associated metabolic engineering objectives. In Chapter 1, we review the state-of-the-art metabolic engineering and synthetic biology tools for pathway and strain design. These tools lay the foundation for the development of the subsequent chapters, which deal in-depth with specific challenges in metabolic pathway design and strain optimization. One of the key challenges in metabolic engineering is to ensure a consistent supply of cofactor, which is often shared across over 100 reactions in a cell, to the desirable biosynthesis reactions. In Chapter 2, we describe the design and optimization of a synthetic metabolic pathway in E. coli to improve the availability of an essential redox cofactor NADPH. The synthetic metabolic pathway was derived from the highly active Entner-Doudoroff (ED) pathway of another prokaryote Zymomonas mobilis. Upon optimizing the expression of the multi-enzyme pathway, we obtained strains with improved NADPH production when compared to the wild-type strain. In addition to NADPH production, the ED pathway also simultaneously generates pyruvate and glyceraldehyde-3-phopshate, which are the precursors for the methylerythritol phosphate (MEP) pathway. As a proof-of-concept, we demonstrated that combination of the synthetic ED pathway and the MEP pathway improved the production of an NADPH-dependent terpenoid by up to 97%. Motivated by the intriguing roles that glycolytic pathways play in a cell and the effects of their perturbation, in Chapter 3, we switch focus to uncover the reason why specific glycolytic pathways prevail in nature despite the presence of alternatives. Although the ED pathway and the Embden-Meyerhof-Parnas (EMP) glycolytic pathways both convert glucose to pyruvate, they take different routes with different intermediates and yield one or two moles of ATP per mole of glucose. Theoretically, one could construct a thermodynamically feasible (i.e., rG

Synthetic Biology and Metabolic Engineering in Plants and Microbes Part B Metabolism in Plants

Synthetic Biology and Metabolic Engineering in Plants and Microbes Part B  Metabolism in Plants Book
Author : Anonim
Publisher : Elsevier
Release : 2016-07-29
ISBN : 0128111232
Language : En, Es, Fr & De

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Book Description :

Synthetic Biology and Metabolic Engineering in Plants and Microbes, Part B, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods, synthetic biology, and metabolic engineering in plants and microbes, and includes sections on such topics as the usage of integrases in microbial engineering, biosynthesis, and engineering of tryptophan derived metabolites, regulation and discovery of fungal natural products, and elucidation and localization of plant pathways. Continues the legacy of this premier serial with quality chapters authored by leaders in the field of enzymology Contains two volumes covering research methods in synthetic biology and metabolic engineering in plants and microbes Includes sections on such topics as the uses of integrases in microbial engineering, biosynthesis and engineering of tryptophan derived metabolites, regulation and discovery of fungal natural products, and elucidation and localization of plant pathways

Biohydrogen

Biohydrogen Book
Author : You-Kwan Oh,Subramanian Mohan Raj,Gyoo Yeol Jung,Sunghoon Park
Publisher : Elsevier Inc. Chapters
Release : 2013-06-11
ISBN : 0128083530
Language : En, Es, Fr & De

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Book Description :

Metabolic engineering has been studied extensively to improve microbial hydrogen (H2) production. Elimination or modification of carbon metabolic pathways, incorporation of nonnative metabolic pathways leading to H2 production, utilization of various carbon substrates, and improvement of H2-producing enzymes (hydrogenase and nitrogenase) were the major focuses of these studies. This chapter highlights recent developments with metabolic engineering toward improving the yield and rate of microbial biohydrogen production.

Engineering Rumen Metabolic Pathways Where We Are and Where Are We Heading

Engineering Rumen Metabolic Pathways  Where We Are  and Where Are We Heading Book
Author : Emilio M. Ungerfeld,C. James Newbold
Publisher : Frontiers Media SA
Release : 2018-03-13
ISBN : 2889454266
Language : En, Es, Fr & De

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Book Description :

Ruminants were domesticated in the Middle East about 10,000 years ago and have since become an inseparable part of human diet, society, and culture. Ruminants can transform inedible plant fiber and non-protein nitrogen into meat, milk, wool and traction, thus allowing human utilization of non-tillable land and industrial by-products. The nutritional flexibility of ruminants is conferred by the rumen´s complex microbial community. Driven by rising income and population growth in emergent economies, the global demand for livestock products, including milk and meat from ruminants, has been increasingly growing, and is predicted to continue growing in the next few decades. The increase in production necessary to satisfy this rising demand is putting much pressure on already dwindling natural resources. There are also concerns about the emissions of methane and nitrous oxide, potent greenhouse gases associated to ruminant production. The need to make ruminant production more efficient in the use of natural resources poses a big challenge to ruminant science, and within it, rumen microbiology. Recent years have seen important advances in basic and applied rumen microbiology and biochemistry. The knowledge generated has significant implications for the efficiency and sustainability of ruminant production and the quality of ruminant products for human health. The present compilation is an update of recent advances in rumen microbiology and ruminant digestion and fermentation, including original research, reviews, and hypothesis and theory articles. We hope that the experimental results, discussion, models and ideas presented herein are useful to foster future research contributing to sustainable ruminant production.

Improved Tools for Engineering Metabolic Pathways in Escherichia Coli

Improved Tools for Engineering Metabolic Pathways in Escherichia Coli Book
Author : Brian Frederick Pfleger
Publisher : Unknown
Release : 2005
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Improved Tools for Engineering Metabolic Pathways in Escherichia Coli book written by Brian Frederick Pfleger, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.