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Mammalian Transcription Analysis And Methods

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Mammalian Transcription Analysis and Methods

Mammalian Transcription Analysis and Methods Book
Author : Ananda Roy
Publisher : Unknown
Release : 2021-06-20
ISBN : 9780444520166
Language : En, Es, Fr & De

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Download Mammalian Transcription Analysis and Methods book written by Ananda Roy, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Mammalian Transcription Analysis and Methods

Mammalian Transcription Analysis and Methods Book
Author : Anonim
Publisher : Unknown
Release : 2020
ISBN : 9780080931135
Language : En, Es, Fr & De

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Gene Transcription

Gene Transcription Book
Author : K. Docherty
Publisher : Wiley
Release : 1996
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

The major recent advances in our understanding of the regulation of gene transcription have stemmed largely from our ability to quantify and characterize mRNA. This book describes the methods used for the analysis of RNA, for DNA transfection into mammalian cells, and reporter technology for mapping transcription control elements. Other techniques used in cloning and characterizing transcription factors and in detailed analysis of regulatory sequences are provided in the companion volume to this book, Gene Transcription: DNA Binding Proteins. The Essential Techniques Series books are designed to provide you with immediate access to the protocols you require every day. These handy pocket-sized manuals are easy to carry around, and conveniently spiral bound making them ideal for lab bench work. Written by experienced laboratory researchers, each book in the Essential Techniques Series gives up-to-date, tried and tested practical information for the life scientist. For each key technique these books: introduce the most commonly used methods, explain the advantages and disadvantages of the methods, and give advice on which procedure to use, provide easy to follow step-by-step protocols, with experimental notes and tips on where to pause, plus information on safety and suppliers.

The Individual Microbe Single Cell Analysis and Agent Based Modelling

The Individual Microbe  Single Cell Analysis and Agent Based Modelling Book
Author : Johan H. J. Leveau,Ferdi L. Hellweger,Jan-Ulrich Kreft,Clara Prats,Weiwen Zhang
Publisher : Frontiers Media SA
Release : 2019-02-19
ISBN : 2889457494
Language : En, Es, Fr & De

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Book Description :

Recent technological advances in single-cell microbiology, using flow cytometry, microfluidics, x-ray fluorescence microprobes, and single-cell -omics, allow for the observation of individuals within populations. Simultaneously, individual-based models (or more generally agent-based models) allow for individual microbes to be simulated. Bridging these techniques forms the foundation of individual-based ecology of microbes (µIBE). µIBE has elucidated genetic and phenotypic heterogeneity that has important consequences for a number of human interests, including antibiotic or biocide resistance, the productivity and stability of industrial fermentations, the efficacy of food preservatives, and the potential of pathogens to cause disease. Individual-based models can help us to understand how these sets of traits of individual microbes influence the above. This eBook compiles all publications from a recent Research Topic in Frontiers in Microbiology. It features recent research where individual observational and/or modelling techniques are applied to gain unique insights into the ecology of microorganisms. The Research Topic “The Individual Microbe: Single-Cell Analysis and Agent-Based Modelling” arose from the 2016 @ASM conference of the same name hosted by the American Society for Microbiology at its headquarters in Washington, D.C. We are grateful to ASM for funding and hosting this conference.

Models and Methods Used in the Analysis of Microarray Expression Data

Models and Methods Used in the Analysis of Microarray Expression Data Book
Author : Dominik Lutter
Publisher : Sudwestdeutscher Verlag Fur Hochschulschriften AG
Release : 2010-06
ISBN : 9783838117638
Language : En, Es, Fr & De

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Book Description :

Modern biology extensively deals with high throughput gene expression data. Although these datasets provide information about the expression of thousands of transcripts, the extraction of information desired by the researcher is a challenging task. Up to now, a variety of different analysis methods and tools have been developed. This book provides an overview of the mechanisms of gene regulation and discusses several of the expression data analysis methods based on the underlying model conceptions. On the basis of three different case studies, distinct analysis approaches are presented and comprehensive discussion of the results is provided.

Sexual Dimorphism in Caenorhabditis Elegans

Sexual Dimorphism in Caenorhabditis Elegans Book
Author : Kara Renee Thoemke
Publisher : Unknown
Release : 2004
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Sexual Dimorphism in Caenorhabditis Elegans book written by Kara Renee Thoemke, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Engineering Mammalian Cells by Exploring Physiological Landscapes

Engineering Mammalian Cells by Exploring Physiological Landscapes Book
Author : Gargi Seth
Publisher : Unknown
Release : 2006
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Engineering Mammalian Cells by Exploring Physiological Landscapes book written by Gargi Seth, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Transcriptional Regulation in Eukaryotes

Transcriptional Regulation in Eukaryotes Book
Author : Michael Carey,Michael F. Carey,Stephen T. Smale
Publisher : CSHL Press
Release : 2000
ISBN : 9780879696351
Language : En, Es, Fr & De

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Book Description :

In the genome era, the analysis of gene expression has become a critical requirement in many laboratories. But there has been no comprehensive source of strategic, conceptual, and technical information to guide this often complex task. Transcriptional Regulation in Eukaryotes answers that need. Written by two experienced investigators, Michael Carey and Stephen Smale at the UCLA School of Medicine, and based in part on the Gene Expression course taught at Cold Spring Harbor Laboratory, this book directly addresses all the concerns of a laboratory studying the regulation of a newly isolated gene and the biochemistry of a new transcription factor. This important and unique book is essential reading for anyone pursuing the analysis of gene expression in model systems or disease states.

Analysis of a Transcriptional Repressor in Drosophila Melanogaster

Analysis of a Transcriptional Repressor in Drosophila Melanogaster Book
Author : Yifan Mao
Publisher : Unknown
Release : 2000
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Analysis of a Transcriptional Repressor in Drosophila Melanogaster book written by Yifan Mao, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Human Molecular Genetics

Human Molecular Genetics Book
Author : Anonim
Publisher : Academic Press
Release : 1996-05-23
ISBN : 0080536417
Language : En, Es, Fr & De

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Book Description :

Human Molecular Genetics is a practical guide to the applications of molecular biology and genetics techniques to human cells. A wide range of experimental procedures for investigating human genes and genomes are presented. * * Mutation Detection in Human Genes - chemical mismatch cleavage, DNA mini-sequencing, SSCP method, RT-PCR, electrophoretic mobility shift assay (EMSA), protein truncation test, chromosome deletion analysis. * Gene Mapping, Cloning, Sequencing - gene linkage determination, large-capacity cloning system, cDNA isolation, differential display method, primer-based DNA sequencing. * Transcription: Promoters, Transcription Factors, mRNA, - promotor mutation analysis, transcription factor identification, mRNA-protein interaction characterization. * RNA Editing, Ribozymes, Antisense RNA-mammalian RNA editing assays, ribozymes as genetic tools, antisense RNA technology. * Genome Recombination, Amplification - recombination assays for mammalian cells, gene amplification measurement. * Receptors, Signal Transduction - intra-cellular receptor characterization, analysis of signal transduction genes. * The Mouse as a Model System for Human Molecular Genetics - mouse genome methods (mouse crosses, somatic cell hybrids, YACs), mouse model for cardiovascular disease.

Handbook of Molecular and Cellular Methods in Biology and Medicine Second Edition

Handbook of Molecular and Cellular Methods in Biology and Medicine  Second Edition Book
Author : Leland J. Cseke,Peter B. Kaufman,Gopi K. Podila,Chung-Jui Tsai
Publisher : CRC Press
Release : 2003-11-24
ISBN : 9780849308154
Language : En, Es, Fr & De

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Book Description :

Since the publication of the best-selling Handbook of Molecular and Cellular Methods in Biology and Medicine, the field of biology has experienced several milestones. Genome sequencing of higher eukaryotes has progressed at an unprecedented speed. Starting with baker's yeast (Saccharomyces cerevisiae), organisms sequenced now include human (Homo sapiens), model crucifer (Arabidopsis thaliana), and rice (Oryza sativa). The invention of DNA microarray technology and advances in bioinformatics have generated vast amounts of genomic data. Reflecting these revolutionary advances Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition documents conventional and modern approaches to tackle scientific research in the post-genomics era. Maintaining the step-by-step format that popularized the first edition, each chapter provides the principles behind the featured method, a detailed description of each protocol, applications of the protocol to different systems, and references for further study. Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition now includes: New protocols in all chapters, including alternative protocols In vitro transcription methods Analysis of DNA sequences New bioseparation techniques New chapters covering: mRNA differential display Inhibition of gene expression In situ hybridization (Localization of gene expression) Combinatorial techniques Computational data mining methods applied to combinatorial chemistry libraries With this book at hand, researchers, teachers, and students can understand and utilize the major techniques and methods currently employed in cellular and molecular biology.

Decoding Mammalian Gene Regulatory Programs Through Efficient Microarray ChIP chip and Sequence Analysis

Decoding Mammalian Gene Regulatory Programs Through Efficient Microarray  ChIP chip and Sequence Analysis Book
Author : Hongkai Ji
Publisher : Unknown
Release : 2007
ISBN : 9780549039952
Language : En, Es, Fr & De

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Book Description :

An empirical hierarchical Bayes approach was proposed to analyze gene expression data collected from microarray experiments. Through a closed-form variance shrinkage estimator, information from multiple genes is pooled to increase the statistical power of multiple hypothesis testing. The approach allows various types of subject matter knowledge to be incorporated conveniently. Caveats in controlling false discovery rate (FDR) will be discussed. When variance shrinkage estimator is employed, inappropriate use of permutations may result in underestimation of FDR.

Analysis of Microarray Data

Analysis of Microarray Data Book
Author : Matthias Dehmer,Frank Emmert-Streib
Publisher : John Wiley & Sons
Release : 2008-09-08
ISBN : 3527622829
Language : En, Es, Fr & De

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Book Description :

This book is the first to focus on the application of mathematical networks for analyzing microarray data. This method goes well beyond the standard clustering methods traditionally used. From the contents: * Understanding and Preprocessing Microarray Data * Clustering of Microarray Data * Reconstruction of the Yeast Cell Cycle by Partial Correlations of Higher Order * Bilayer Verification Algorithm * Probabilistic Boolean Networks as Models for Gene Regulation * Estimating Transcriptional Regulatory Networks by a Bayesian Network * Analysis of Therapeutic Compound Effects * Statistical Methods for Inference of Genetic Networks and Regulatory Modules * Identification of Genetic Networks by Structural Equations * Predicting Functional Modules Using Microarray and Protein Interaction Data * Integrating Results from Literature Mining and Microarray Experiments to Infer Gene Networks The book is for both, scientists using the technique as well as those developing new analysis techniques.

Analysis of the Controlling Elements for Transcription of the CHO APRT Gene

Analysis of the Controlling Elements for Transcription of the CHO APRT Gene Book
Author : Bin-Ru She
Publisher : Unknown
Release : 1993
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Analysis of the Controlling Elements for Transcription of the CHO APRT Gene book written by Bin-Ru She, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Omics Approaches in Breast Cancer

Omics Approaches in Breast Cancer Book
Author : Debmalya Barh
Publisher : Springer
Release : 2014-10-13
ISBN : 8132208439
Language : En, Es, Fr & De

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Book Description :

Breast cancer is the most common cancer in females that accounts for highest cancer specific deaths worldwide. In the last few decades research has proven that breast cancer can be treated if diagnosed at early stages and proper therapeutic strategy is adopted. Omics-based recent approaches have unveiled the molecular mechanism behind the breast tumorigenesis and aid in identification of next-generation molecular markers for early diagnosis, prognosis and even the effective targeted therapy. Significant development has taken place in the field of omics in breast cancer in the last decade. The most promising omics approaches and their outcomes in breast cancer have been presented in this book for the first time. The book covers omics technologies and budding fields such as breast cancer miRNA, lipidomics, epigenomics, proteomics, nutrigenomics, stem cell, pharmacogenomics and personalized medicine and many more along with conventional topics such as breast cancer management etc. It is a research-based reference book useful for clinician-scientists, researchers, geneticists and health care industries involved in various aspects of breast cancer. The book will also be useful for students of biomedicine, pathology and pharmacy.

Transcription and Translation

Transcription and Translation Book
Author : B. D. Hames,S. J. Higgins,Steve J. Higgins
Publisher : Irl Press
Release : 1984
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Transcription and Translation book written by B. D. Hames,S. J. Higgins,Steve J. Higgins, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Proceedings of the National Academy of Sciences of the United States of America

Proceedings of the National Academy of Sciences of the United States of America Book
Author : National Academy of Sciences (U.S.)
Publisher : Unknown
Release : 2007
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Proceedings of the National Academy of Sciences of the United States of America book written by National Academy of Sciences (U.S.), available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.