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Livestock Genome Editing Tools

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Genome Editing Tools and Gene Drives

Genome Editing Tools and Gene Drives Book
Author : Reagan Mudziwapasi,Taylor & Francis Group,Ringisai Chekera,Clophas Zibusiso Ncube,Irvonnie Shoko,Berlinda Ncube,Thandanani Moyo,Jeffrey Godfrey Chimbo,Jemethious Dube,Farai Faustos Mashiri,Moira Amanda Mubani,Duncan Maruta,Charity Chimbo,Mpumuzi Masuku,Ryman Shoko,Rutendo Patricia Nyamusamba,Fortune Ntengwa Jomane
Publisher : CRC Press
Release : 2021-10-19
ISBN : 9780367760663
Language : En, Es, Fr & De

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Book Description :

Genome editing methods are becoming routine tools for molecular and cell biologists. Such tools include ZFNs, CRISPR, megaTALs and TALENs. These tools are revolutionizing the creation of precisely manipulated genomes to modify the characteristics of organisms or cells. Additionally, gene drives have altered the way we understand inheritance laws. They give us the ability to have total control of the inheritance of traits of choice and importance. This succinct volume summarizes the history, principles, and applications - as well as the advantages and disadvantages - of each of these tools and various kinds of gene drives. The book is part of a program to produce books helpful to students and faculties of science at colleges and universities. This volume in the Pocket Guides to Biomedical Sciences series will help demystify these technologies. The book fills the gap between established conventional methods and the novel and exciting newly introduced tools of genome editing and gene drives. It will help young scientists understand the emerging genome editing tools and gene drives thereby promoting related research and adoption. Key Features The book extensively reviews the current genome editing tools and gene drives. Clarifies their targeting mechanisms and specificity of genome editing tools. Details many different types of natural and synthetic gene drives. Highlights concerns with gene drives and genome editing tools. Related Titles Brown, T. A. Genomes 4 (ISBN 978-0-8153-4508-4) Soh, J., et al. Genome Annotation (ISBN 978-1-4398-4117-4) Samuelsson, T. The Human Genome in Health and Disease: A Story in Four Letters (ISBN 978-0-8153-4591-6)

Targeted Genome Editing Using Site Specific Nucleases

Targeted Genome Editing Using Site Specific Nucleases Book
Author : Takashi Yamamoto
Publisher : Springer
Release : 2015-01-05
ISBN : 4431552278
Language : En, Es, Fr & De

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Book Description :

This book serves as an introduction to targeted genome editing, beginning with the background of this rapidly developing field and methods for generation of engineered nucleases. Applications of genome editing tools are then described in detail, in iPS cells and diverse organisms such as mice, rats, marine invertebrates, fish, frogs, and plants. Tools that are mentioned include zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR/Cas9, all of which have received much attention in recent years as breakthrough technologies. Genome editing with engineered nucleases allows us to precisely change the target genome of living cells and is a powerful way to control functional genes. It is feasible in almost all organisms ranging from bacteria to plants and animals, as well as in cultured cells such as ES and iPS cells. Various genome modifications have proven successful, including gene knockout and knock-in experiments with targeting vectors and chromosomal editing. Genome editing technologies hold great promise for the future, for example in biomedical research, clinical medicine, and generation of crops and livestock with desirable traits. A wide range of readers will find this book interesting, and with its focus on applications in a variety of organisms and cells, the book will be valuable for life scientists in all fields.

Advances in Animal Genomics

Advances in Animal Genomics Book
Author : Sukanta Mondal,Ram Lakhan Singh
Publisher : Academic Press
Release : 2020-11-25
ISBN : 0128206128
Language : En, Es, Fr & De

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Book Description :

Advances in Animal Genomics provides an outstanding collection of integrated strategies involving traditional and modern - omics (structural, functional, comparative and epigenomics) approaches and genomics-assisted breeding methods which animal biotechnologists can utilize to dissect and decode the molecular and gene regulatory networks involved in the complex quantitative yield and stress tolerance traits in livestock. Written by international experts on animal genomics, this book explores the recent advances in high-throughput, next-generation whole genome and transcriptome sequencing, array-based genotyping, and modern bioinformatics approaches which have enabled to produce huge genomic and transcriptomic resources globally on a genome-wide scale. This book is an important resource for researchers, students, educators and professionals in agriculture, veterinary and biotechnology sciences that enables them to solve problems regarding sustainable development with the help of current innovative biotechnologies. Integrates basic and advanced concepts of animal biotechnology and presents future developments Describes current high-throughput next-generation whole genome and transcriptome sequencing, array-based genotyping, and modern bioinformatics approaches for sustainable livestock production Illustrates integrated strategies to dissect and decode the molecular and gene regulatory networks involved in complex quantitative yield and stress tolerance traits in livestock Ensures readers will gain a strong grasp of biotechnology for sustainable livestock production with its well-illustrated discussion

Animal Biotechnology 2

Animal Biotechnology 2 Book
Author : Heiner Niemann,Christine Wrenzycki
Publisher : Springer
Release : 2018-08-01
ISBN : 331992348X
Language : En, Es, Fr & De

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Book Description :

This two-volume textbook provides a comprehensive overview on the broad field of Animal Biotechnology with a special focus on livestock reproduction and breeding. The reader will be introduced to a variety of state-of-the-art technologies and emerging genetic tools and their applications in animal production. Also, ethics and legal aspects of animal biotechnology will be discussed and new trends and developments in the field will be critically assessed. The two-volume work is a must-have for graduate students, advanced undergraduates and researchers in the field of veterinary medicine, genetics and animal biotechnology. This second volume is dedicated to genetic tools in animal biotechnology such as somatic cloning, transgenic technologies and the application of stem cells in livestock breeding. Also, ethics and legal aspects are discussed.

Genome Editing Tools and Gene Drives

Genome Editing Tools and Gene Drives Book
Author : Reagan Mudziwapasi,Ringisai Chekera,Clophas Zibusiso Ncube,Irvonnie Shoko,Berlinda Ncube,Thandanani Moyo,Jeffrey Godfrey Chimbo,Jemethious Dube,Farai Faustos Mashiri,Moira Amanda Mubani,Duncan Maruta,Charity Chimbo,Mpumuzi Masuku,Ryman Shoko,Rutendo Patricia Nyamusamba,Fortune Ntengwa Jomane
Publisher : CRC Press
Release : 2021-10-19
ISBN : 1000463109
Language : En, Es, Fr & De

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Book Description :

Genome-editing methods are becoming routine tools for molecular and cell biologists. Such tools include ZFNs, CRISPR, megaTALs and TALENs. These tools are revolutionizing the creation of precisely manipulated genomes to modify the characteristics of organisms or cells. Additionally, gene drives have altered the way we understand inheritance laws. They give us the ability to have total control of the inheritance of traits of choice and importance. This succinct volume summarizes the history, principles and applications – as well as the advantages and disadvantages – of each of these tools and various kinds of gene drives. The book is part of a program to produce books helpful to students and faculties of science at colleges and universities. This volume in the Pocket Guides to Biomedical Sciences series will help demystify these technologies. The book fills the gap between established conventional methods and the novel and exciting newly introduced tools of genome editing and gene drives. It will help young scientists understand the emerging genome-editing tools and gene drives, thereby promoting related research and adoption. Key Features Extensively reviews the current genome-editing tools and gene drives Clarifies the targeting mechanisms and specificity of genome-editing tools Details many different types of natural and synthetic gene drives Highlights concerns with gene drives and genome-editing tools Related Titles Brown, T. A. Genomes 4 (ISBN 978-0-8153-4508-4) Samuelsson, T. The Human Genome in Health and Disease: A Story in Four Letters (ISBN 978-0-8153-4591-6) Soh, J., et al. Genome Annotation (ISBN 978-1-4398-4117-4)

CRISPR Cas Systems

CRISPR Cas Systems Book
Author : Rodolphe Barrangou,John van der Oost
Publisher : Springer Science & Business Media
Release : 2012-12-13
ISBN : 364234657X
Language : En, Es, Fr & De

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Book Description :

CRISPR/Cas is a recently described defense system that protects bacteria and archaea against invasion by mobile genetic elements such as viruses and plasmids. A wide spectrum of distinct CRISPR/Cas systems has been identified in at least half of the available prokaryotic genomes. On-going structural and functional analyses have resulted in a far greater insight into the functions and possible applications of these systems, although many secrets remain to be discovered. In this book, experts summarize the state of the art in this exciting field.

Emerging Issues in Climate Smart Livestock Production

Emerging Issues in Climate Smart Livestock Production Book
Author : Sukanta Mondal,Ram Lakhan Singh
Publisher : Academic Press
Release : 2021-12-15
ISBN : 0128222662
Language : En, Es, Fr & De

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Book Description :

Emerging Issues in Climate Smart Livestock Production: Biological Tools and Techniques furnishes a detailed reference on livestock sustainability and the role of biotechnology for creating more sustainable livestock production systems. The book is a collection of scientific techniques, including genetic engineering used to modify and improve animals, fishes, and microorganisms for human benefit. The book is particularly attractive for scientists, researchers, students, educators, and professionals in agriculture, veterinary, and biotechnology science. This book promotes several biotechnological approaches that can easily be evaluated in the field for quality assurance programs beneficial to producing livestock products and overall public health. Biotechnology has the potential to improve the productivity of animals via increased growth, carcass quality and reproduction, improved nutrition and feed utilization, improved food quality and safety, improved animal health and welfare, and reduced waste through more efficient utilization of resources. Identifies and explores biotechnological approaches for sustainable livestock and fish production Focuses on strategies for enhancing livestock and fishery productivity and sustainability Presents the latest research on modern methods and technologies

Genome Editing Applications in Animal Research

Genome Editing Applications in Animal Research Book
Author : Jun Song,Bradford Daigneault,Zhanjun Li,Huaqiang Yang
Publisher : Frontiers Media SA
Release : 2022-02-18
ISBN : 2889744426
Language : En, Es, Fr & De

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Book Description :

Download Genome Editing Applications in Animal Research book written by Jun Song,Bradford Daigneault,Zhanjun Li,Huaqiang Yang, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Genome Editing in Animals

Genome Editing in Animals Book
Author : Izuho Hatada
Publisher : Humana Press
Release : 2017-06-23
ISBN : 9781493971275
Language : En, Es, Fr & De

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Book Description :

This volume details protocols that can be used for generation of knockout animals. Chapters guide the reader through basic protocols for three genome editing technologies, target design tools, and specific protocols for each animal. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Genome Editing in Animals: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Sustainable Agriculture Reviews 57

Sustainable Agriculture Reviews 57 Book
Author : Vinod Kumar Yata,Ashok Kumar Mohanty,Eric Lichtfouse
Publisher : Springer Nature
Release : 2022-09-23
ISBN : 3031074963
Language : En, Es, Fr & De

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Book Description :

This 2nd book provides fundamental concepts and recent applications of biotechnological methods, such as genetic selection, breeding methods and genetic engineering tools. Biotechnology has remarkably improved the productivity of livestock by increasing the reproduction efficiency and decreasing the generation time. The chapters detail the mechanisms of methods for animal reproduction and breeding methods. This book focus on the impact of minerals, steroids metabolic stress, nutritional stress and anti-nutritional factors on the livestock reproduction.

Gene Editing Stem Cells and Chimeras in Sheep Embryos

Gene Editing  Stem Cells and Chimeras in Sheep Embryos Book
Author : Marcela Vilarino Pareda
Publisher : Unknown
Release : 2018
ISBN : 9780438628861
Language : En, Es, Fr & De

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Book Description :

Genome editing has become the most used tool to produce genetically engineered animals. Specifically in livestock species, the CRISPR/Cas9 system has allowed hundreds of animal models in the last three years to be generated. Despite the high efficiency of this genetic tool, there are still deficiencies of the system that compromise the infinite number of possibilities to induce complex genetic modifications. In vitro studies, as well as intense mouse embryo work paved the way for all the experiments that are currently performed in livestock. However, specific studies in livestock species are required to optimize and explore different possibilities of the system. The sheep is an accepted biomedical model for the scientific community, it also has a comparable size to humans and a well understood physiology that is reflected in the efficient platform for assisted reproductive technologies that are established for this species. The possibility of using sheep as hosts for human organ generation is the main drive of this dissertation. In order to explore this idea, a series of experiments were performed approaching the two critical steps that would be required: generating the “niche” in the host animals and efficiently producing chimeras. We first sought to confirm the possibility of creating a developmental niche by inducing a PDX1-KO using the CRISPR/Cas9 system for generating an apancreatic sheep (Chapter 2). Next, we did a series of experiments to refine the system intending to improve the production of KO sheep using PDX1 as the target gene (Chapter 3). To test the possibility of creating human-sheep chimeras we tried different human iPSCs conditions in sheep embryos. From these studies we found that inhibition of apoptosis increases human chimera contribution in sheep embryos (Chapter 4). Additionally, we developed sheep ESCs that maintain a stable morphology, karyotype and pluripotency markers expression for over 40 passages. These cells represent a promising tool for gene editing experiments as well as further chimera studies that will help to understand pluripotency in livestock species as well as conditions in sheep that lead to chimera contribution (Chapter 5). Collectively, this work intended to improve the use of livestock species as biomedical and agricultural models.

Safety of Genetically Engineered Foods

Safety of Genetically Engineered Foods Book
Author : National Research Council,Institute of Medicine,Board on Agriculture and Natural Resources,Food and Nutrition Board,Board on Life Sciences,Committee on Identifying and Assessing Unintended Effects of Genetically Engineered Foods on Human Health
Publisher : National Academies Press
Release : 2004-07-08
ISBN : 9780309166157
Language : En, Es, Fr & De

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Book Description :

Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.

Advances in Animal Biotechnology

Advances in Animal Biotechnology Book
Author : Birbal Singh,Gorakh Mal,Sanjeev K. Gautam,Manishi Mukesh
Publisher : Springer
Release : 2019-07-09
ISBN : 3030213099
Language : En, Es, Fr & De

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Book Description :

This book entitled, “Advances in Animal Biotechnology,” is a compilation of state-of-the-art in the field of Animal Biotechnology including fishery, that are not sheltered in depth in earlier publications. It offers an update on avant-garde technologies and advances in key aspects of genetic engineering, metagenomics, assisted reproduction, animal genomics, biotechnology in veterinary health, as well as the role of gut and marine microbial ecosystems in livestock and industrial development. The book is divided broadly into five different sections, viz., Gut Microbiome and Nutritional Biotechnology, Assisted Reproduction Biotechnology, Livestock Genomics, Health Biotechnology, and Animal Biotechnology in Global Perspective. The book covers the syllabi of Animal Biotechnology courses in various universities, academia and competitive examinations at various levels. Researchers, Continuing Graduates, and Academicians, Research Institutions, and Biotech Companies will be benefited from this valuable compilation of research. Its broad spectrum makes this work a valuable resource for professionals, researchers, academics and students in the field of veterinary and animal production as well as the biotechnology industry.

Heritable Human Genome Editing

Heritable Human Genome Editing Book
Author : The Royal Society,National Academy of Sciences,National Academy of Medicine,International Commission on the Clinical Use of Human Germline Genome Editing
Publisher : National Academies Press
Release : 2021-01-16
ISBN : 0309671132
Language : En, Es, Fr & De

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Book Description :

Heritable human genome editing - making changes to the genetic material of eggs, sperm, or any cells that lead to their development, including the cells of early embryos, and establishing a pregnancy - raises not only scientific and medical considerations but also a host of ethical, moral, and societal issues. Human embryos whose genomes have been edited should not be used to create a pregnancy until it is established that precise genomic changes can be made reliably and without introducing undesired changes - criteria that have not yet been met, says Heritable Human Genome Editing. From an international commission of the U.S. National Academy of Medicine, U.S. National Academy of Sciences, and the U.K.'s Royal Society, the report considers potential benefits, harms, and uncertainties associated with genome editing technologies and defines a translational pathway from rigorous preclinical research to initial clinical uses, should a country decide to permit such uses. The report specifies stringent preclinical and clinical requirements for establishing safety and efficacy, and for undertaking long-term monitoring of outcomes. Extensive national and international dialogue is needed before any country decides whether to permit clinical use of this technology, according to the report, which identifies essential elements of national and international scientific governance and oversight.

CRISPR in Animals and Animal Models

CRISPR in Animals and Animal Models Book
Author : Anonim
Publisher : Academic Press
Release : 2017-11-10
ISBN : 0128125071
Language : En, Es, Fr & De

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Book Description :

CRISPR in Animals and Animal Models, Volume 152, the latest release in the Progress in Molecular Biology and Translational Science series, explores the genome editing CRISPR system in cells and animal models, its applications, the uses of the CRISPR system, and the past, present and future of CRISPR genome editing. Topics of interest in this updated volume include a section on CRISPR history, The genome editing revolution, Programming CRISPR and its applications, CRISPR Delivery methods, CRISPR libraries and screening, CRISPR investigation in haploid cells, CRISPR in the generation of transgenic animals, CRISPR therapeutics, and Promising strategies and present challenges. Accessible to students and researchers alike Written by leading authorities in the field

Animal Genomics

Animal Genomics Book
Author : Bhanu P. Chowdhary
Publisher : S Karger Ag
Release : 2003
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

This publication provides an update on the current status of gene maps in different livestock and pet/companion animal species. The findings summarized in species specific commentaries and original articles testify the rapid advances made in the field of animal genomics. Of significant interest is the fact that current investigations are providing headways for two important and exciting research fronts: targeted high-resolution mapping leading to the application of genomic information in addressing questions of economic and biological significance in animals, and the initiation of whole genome sequencing projects for some of the animal species. Like in humans and mice, this will set the stage for a new level of research and real time complex analysis of the genomes of these species. Animal Genomics signifies the beginning of a new era in this field and celebrates the achievements of the past 20 years of genomics research. It will be of special interest to researchers involved in genome analysis - both gross chromosomal as well as molecular - in various animal species, and to comparative and evolutionary geneticists.

Gene Editing

Gene Editing Book
Author : Yuan-Chuan Chen,Shiu-Jau Chen
Publisher : BoD – Books on Demand
Release : 2019-05-29
ISBN : 1789845084
Language : En, Es, Fr & De

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Book Description :

Gene-editing technologies (e.g., ZFNs, TALENs, and CRISPRs/Cas9) have been extensively used as tools in basic research. They are further applied in manufacturing agricultural products, food, industrial products, medicinal products, etc. Particularly, the discovery of medicinal products using gene-editing technologies will open a new era for human therapeutics. Though there are still many technical and ethical challenges ahead of us, more and more products based on gene-editing technologies have been approved for marketing. These technologies are promising for multiple applications. Their development and implications should be explored in the broadest context possible. Future research directions should also be highlighted. In this book, the applications, perspectives, and challenges of gene-editing technologies are significantly demonstrated and discussed.

Applications of Genome Modulation and Editing

Applications of Genome Modulation and Editing Book
Author : Paul John Verma,Huseyin Sumer,Jun Liu
Publisher : Springer Nature
Release : 2022-06-13
ISBN : 107162301X
Language : En, Es, Fr & De

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Book Description :

This volume details generation of gene-edited cell lines and organisms as models for human diseases, pest control, and large animal welfare and production outcomes. Chapters guide readers through gene regulation, editing, screening of cell lines, genome editing, and an overview of the tools for efficient genome editing including; ZFNs, TALENs, and CRISPR. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step- by-step, readily reproducible protocols. Authoritative and cutting-edge, Applications of Genome Modulation and Editing aims to be a useful and practical guide for researchers to commence or advance their study in this field.

Agricultural Biotechnology Biodiversity and Bioresources Conservation and Utilization

Agricultural Biotechnology  Biodiversity and Bioresources Conservation and Utilization Book
Author : Olawole O. Obembe,Emmanuel Olufemi Ekundayo,Arinze Stanley Okoli,Abubakar Gidado,Charles Oluwaseun Adetunji,Abdulrazak B. Ibrahim,Benjamin Ewa Ubi
Publisher : CRC Press
Release : 2022-05-10
ISBN : 1000550923
Language : En, Es, Fr & De

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Book Description :

This book covers a range of important topics on crop and animal genetics, breeding and genomics, as well as biodiversity and genetic resources conservation and utilization reflecting three thematic sections of working groups of the Biotechnology Society of Nigeria. The topics range from agricultural biotechnology, including genetically modified organisms and gene-editing for agronomically important traits in tropical crops, to Nigeria’s mega biodiversity and genetic resources conservation. This book will engender a deeper understanding of underpinning mechanisms, technologies, processes and science–policy nexus that has placed Nigeria as a leader in biotechnology in Africa. The book will be useful reference material for scientists and researchers working in the fields of food and agricultural biotechnology, bioinformatics, plant and animal genetics, breeding and genomics, genetic resources conservation and enhancement. Emphasizes recent advances in biotechnologies that could ameliorate the high-level global food and nutrition insecurity through plant and animal genetics, breeding, as well as genomics Provides detailed information towards harnessing indigenous bioresources for food and nutrition security and climate change adaptation Introduces new frontiers in the area of genomics, most especially their relevant applications in crop and animal breeding Reviews biotechniques that could enhance plant genetic resources conservation and utilization Discusses current biotechnological approaches to exploit genetic resources including the development of synthetic hexaploid wheat (SHW) for crop adaptation to the increasingly changing global climate Olawole O. Obembe, Ph.D., is a Professor of Plant Biotechnology and UNESCO Chair, Plant Biotechnology, Covenant University Ota, Nigeria. Emmanuel Olufemi Ekundayo, Ph.D., is Associate Professor of Medical Microbiology and Microbial Genetics, Michael Okpara University of Agriculture, Umudike, Nigeria. Arinze Stanley Okoli, Ph.D., is Associate Professor at Genoek – Centre for Biosafety, Universitetet II, Breivika, Tromsoe, Norway. Abubakar Gidado, Ph.D., is Professor of Biochemistry and Director North-East Zonal Biotechnology Centre of Excellence at the University of Maiduguri, Nigeria. Charles Oluwaseun Adetunji, Ph.D., is Associate Professor of Microbiology and Biotechnology and Director of Intellectual Property and Technology Transfer, Edo State University, Uzairue, Nigeria. Abdulrazak B. Ibrahim, Ph.D., is a Capacity Development Expert at the Forum for Agricultural Research in Africa (FARA) and Associate Professor of Biochemistry, Ahmadu Bello University, Zaria, Nigeria. Benjamin Ewa Ubi, Ph.D., is a Professor of Plant Breeding and Biotechnology and Director, Biotechnology Research and Development Centre, Ebonyi State University Abakaliki, Nigeria.

Genome Editing Tools and Gene Drives

Genome Editing Tools and Gene Drives Book
Author : Reagan Mudziwapasi
Publisher : Unknown
Release : 2021-10-19
ISBN : 9780367745967
Language : En, Es, Fr & De

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Book Description :

This book succinctly reviews and describes genome editing tools such as ZFNs, CRISPR, megaTALs and TALENs. It covers the history, principles, applications and the advantages and disadvantages of each tool. The book provides an approachable outline enhancing science education in schools and universities by demystifying genome editing technologies.