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Protein Liquid Chromatography

Protein Liquid Chromatography Book
Author : M. Kastner
Publisher : Elsevier
Release : 2000
ISBN : 9780444502117
Language : En, Es, Fr & De

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Book Description :

Protein Liquid Chromatography is a handbook-style guide to liquid chromatography as a tool for isolating and purifying proteins, consisting of 25 individual chapters divided into three parts: Part A covers commonly-used, classic modes of chromatography such as ion-exchange, size-exclusion, and reversed-phase; Part B deals with various target protein classes such as membrane proteins, recombinant proteins, and glycoproteins; and Part C looks at various miscellaneous related topics, including coupling reaction, buffer solution additives, and software. The text as a whole can be viewed as a systematic survey of available methods and how best to use them, but also attempts to provide an exhaustive coverage of each facet. How to solve a specific problem using a chosen method is the overall essence of the volume. The principle philosophy of this compilation is that practical application is everything; therefore, both classical and modern methods are presented in detail, with examples involving conventional, medium- and high-pressure techniques. Over-exposure to history, concept, and theory has deliberately been avoided. The reader will find a wealth of tips and tricks from users for users, including advice on the advantages and disadvantages of each method. Easy-to-read sections on "Getting started now" and "Where to go from here" attempt to provide hands-on, fool-proof detailed practical procedures with complete and even standard model runs for any scientist or technician at work in this area.

Introduction to Modern Liquid Chromatography

Introduction to Modern Liquid Chromatography Book
Author : Lloyd R. Snyder,Joseph J. Kirkland,John W. Dolan
Publisher : John Wiley & Sons
Release : 2011-09-20
ISBN : 1118210395
Language : En, Es, Fr & De

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Book Description :

The latest edition of the authoritative reference to HPLC High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. This carefully considered Third Edition maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics as: The basis of HPLC separation and the general effects of different experimental conditions Equipment and detection The column—the "heart" of the HPLC system Reversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniques Computer simulation, qualitative and quantitative analysis, and method validation and quality control The separation of large molecules, including both biological and synthetic polymers Chiral separations, preparative separations, and sample preparation Systematic development of HPLC separations—new to this edition Troubleshooting tricks, techniques, and case studies for both equipment and chromatograms Designed to fulfill the needs of the full range of HPLC users, from novices to experts, Introduction to Modern Liquid Chromatography, Third Edition offers the most up-to-date, comprehensive, and accessible survey of HPLC methods and applications available.

Liquid Chromatography of Natural Pigments and Synthetic Dyes

Liquid Chromatography of Natural Pigments and Synthetic Dyes Book
Author : Tibor Cserháti
Publisher : Elsevier
Release : 2006-12-07
ISBN : 9780080465760
Language : En, Es, Fr & De

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Book Description :

This latest volume in the series entitled Liquid Chromatography of Natural Pigments and Synthetic Dyes presents an overview of the latest developments in the field while critically evaluating this method of analysis and providing comparisons of the various liquid chromatographic separation techniques that are currently available. Natural pigments and synthetic dyes are extensively used in various fields of everyday life including food production, textile industry, paper production, agricultural practice and research and water science and technology. Besides their capacity for increasing the marketability of products, natural pigments have shown advantageous biological activity as antioxidants and anticancer agents. On the negative side, synthetic pigments have a significant impact on the environment and can cause adverse toxicological side effects. Both pigment classes exhibit considerable structural diversity. As the stability of the pigments against hydrolysis, oxidation and other environmental and technological conditions is markedly different, the exact determination of the pigment composition may help for the prediction of the shelf-life of products and the assessment of the influence of technological steps on the pigment fractions resulting in more consumer friend processing methods. Furthermore, the qualitative determination and identification of the pigments may contribute to the establishment of the provenance of the product. The unique separation capacity of liquid chromatographic (LC) techniques makes it a method of preference for the analysis of pigments in any complicated accompanying matrices. * an overview of the latest developments in the field * a critical evaluation of results from this form of analysis * a comparison of the various LC (liquid chromatographic) separation techniques * future trends in the LC analysis of pigments

Applications of High Performance Liquid Chromatography

Applications of High Performance Liquid Chromatography Book
Author : S. Pryde,M.T. Gilbert
Publisher : Springer Science & Business Media
Release : 1979-01-31
ISBN : 9780412142208
Language : En, Es, Fr & De

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Book Description :

Download Applications of High Performance Liquid Chromatography book written by S. Pryde,M.T. Gilbert, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Liquid Chromatography

Liquid Chromatography Book
Author : Salvatore Fanali,Paul R. Haddad,Colin Poole,Marja-Liisa Riekkola
Publisher : Elsevier
Release : 2017-06-23
ISBN : 0128093447
Language : En, Es, Fr & De

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Book Description :

Liquid Chromatography: Applications, Second Edition,is a single source of authoritative information on all aspects of the practice of modern liquid chromatography. It gives those working in both academia and industry the opportunity to learn, refresh, and deepen their knowledge of the wide variety of applications in the field. In the years since the first edition was published, thousands of papers have been released on new achievements in liquid chromatography, including the development of new stationary phases, improvement of instrumentation, development of theory, and new applications in biomedicine, metabolomics, proteomics, foodomics, pharmaceuticals, and more. This second edition addresses these new developments with updated chapters from the most expert researchers in the field. Emphasizes the integration of chromatographic methods and sample preparation Explains how liquid chromatography is used in different industrial sectors Covers the most interesting and valuable applications in different fields, e.g., proteomic, metabolomics, foodomics, pollutants and contaminants, and drug analysis (forensic, toxicological, pharmaceutical, biomedical) Includes references and tables with commonly used data to facilitate research, practical work, comparison of results, and decision-making

Liquid Chromatography

Liquid Chromatography Book
Author : Salvatore Fanali,Paul R. Haddad,Colin Poole,Marja-Liisa Riekkola
Publisher : Elsevier
Release : 2017-06-22
ISBN : 0128093455
Language : En, Es, Fr & De

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Book Description :

Liquid Chromatography: Fundamentals and Instrumentation, Second Edition, is a single source of authoritative information on all aspects of the practice of modern liquid chromatography. It gives those working in both academia and industry the opportunity to learn, refresh, and deepen their understanding of new fundamentals and instrumentation techniques in the field. In the years since the first edition was published, thousands of papers have been released on new achievements in liquid chromatography, including the development of new stationary phases, improvement of instrumentation, development of theory, and new applications in biomedicine, metabolomics, proteomics, foodomics, pharmaceuticals, and more. This second edition addresses these new developments with updated chapters from the most expert researchers in the field. Emphasizes the integration of chromatographic methods and sample preparation Explains how liquid chromatography is used in different industrial sectors Covers the most interesting and valuable applications in different fields, e.g., proteomic, metabolomics, foodomics, pollutants and contaminants, and drug analysis (forensic, toxicological, pharmaceutical, biomedical) Includes references and tables with commonly used data to facilitate research, practical work, comparison of results, and decision-making

High Performance Liquid Chromatography

High Performance Liquid Chromatography Book
Author : Mary T. Gilbert
Publisher : Elsevier
Release : 2013-10-22
ISBN : 1483281299
Language : En, Es, Fr & De

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Book Description :

High Performance Liquid Chromatography focuses on the developments, operating techniques, practices, equipment, and packing materials involved in High Performance Liquid Chromatography (HPLC). The book first offers information on basic chromatographic theory, equipment, and the column. Topics include resolution, efficiency, pumps and gradient systems, connectors, detectors, injectors, column packing and testing, packing materials, and coupling of columns. The text also ponders on sample treatment and separation methods, as well as trace analysis, reversed phase chromatography, and selection/optimization conditions. The publication examines adjustment of selectivity by the use of eluent additives and preparative liquid chromatography. Discussions focus on chromatography on dynamically modified oxide gels, metal complexation, crown ethers, ion pair chromatography, materials for preparative chromatography, and separation strategy. The text also reviews the trends in the practice of HPLC and chiral chromatography. The book is a dependable reference for readers interested in High Performance Liquid Chromatography.

High Performance Liquid Chromatography

High Performance Liquid Chromatography Book
Author : Csaba Horváth
Publisher : Elsevier
Release : 2013-10-22
ISBN : 1483261832
Language : En, Es, Fr & De

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Book Description :

High-Performance Liquid Chromatography: Advances and Perspectives, Volume 2 presents the fundamental aspects of high-performance liquid chromatography, laboratory technique for chemical analysis with a wide range of applications. The book consists of three chapters discussing the optimization of the column and the operating conditions of the chromatographic system; use of polar adsorbents and nonpolar eluents; and reversed-phase chromatography, the main branch of high-performance liquid chromatography. Chromatographers, chemists, and researchers in the field of chemical analysis will find this book a good reference material.

High Performance Liquid Chromatography in Phytochemical Analysis

High Performance Liquid Chromatography in Phytochemical Analysis Book
Author : Monika Waksmundzka-Hajnos,Joseph Sherma
Publisher : CRC Press
Release : 2010-11-08
ISBN : 9781420092615
Language : En, Es, Fr & De

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Book Description :

The powerful, efficient technique of high performance liquid chromatography (HPLC) is essential to the standardization of plant-based drugs, identification of plant material, and creation of new herbal medicines. Filling the void in this critical area, High Performance Liquid Chromatography in Phytochemical Analysis is the first book to give a comp

Liquid Chromatography

Liquid Chromatography Book
Author : L.R. Snyder,J.W. Dolan
Publisher : Elsevier Inc. Chapters
Release : 2013-01-08
ISBN : 0128071346
Language : En, Es, Fr & De

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Book Description :

Liquid-solid or normal-phase chromatography is employed for the separation of non-ionic solutes by adsorption onto the surface of an inorganic oxide stationary phase and displacement by competition with the components of the mobile phase. It has particular advantages for compound-class separations and the separation of diasteromers and geometrical isomers soluble in organic solvents. Common stationary phases for high-performance liquid chromatography include silica and chemically-bonded silica phases with 3-cyanopropyl, 3-aminopropyl, and spacer bonded propanediol groups. The use of eluotropic series for mobile phase selection are described as well as general strategies for method development.

High Performance Liquid Chromatography

High Performance Liquid Chromatography Book
Author : Sandie Lindsay,Barnes,John Barnes,ACOL (Project)
Publisher : John Wiley & Sons
Release : 1992-07-27
ISBN : 9780471931157
Language : En, Es, Fr & De

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Book Description :

Part of a series of textbooks concerned with the training, continuing education and updating of all technical staff concerned with analytical chemistry, this volume explores a powerful chromatographic technique which has arisen from research carried out on liquid and gas chromatography.

Liquid Chromatography Mass Spectrometry

Liquid Chromatography Mass Spectrometry Book
Author : Alfred L. Yergey,Charles G. Edmonds,Ivor A.S. Lewis,Marvin L. Vestal
Publisher : Springer Science & Business Media
Release : 2013-12-14
ISBN : 148993605X
Language : En, Es, Fr & De

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Book Description :

This book is intended both to be an introduction to techniques and applications of liquid chromatography/mass spectrometry and to serve as a reference for future workers. When we undertook its writing, we chose not to cover the field, particularly applications, exhaustively. Rather we wished to produce a book that would be of use to people just beginning to use the technique as well as to more advanced practitioners. In this regard, we have sought to highlight techniques and applications that are of current importance, while not neglecting descriptions of approaches that may be of significance in the future. We hope that we have succeeded in this. At the same time we hope that the bibliography, with indexes classified by author and title, will make this book of value to those who may disagree with our emphasis. ACKNOWLEDGMENTS. One of us (C. G. E. ) wishes to acknowledge the en couragment of Professor J. A. McCloskey in undertaking this project. All four of us are grateful for the continuous and expert assistance of V. A. Edmonds in the preparation of the Bibliography. Alfred L. Y ergey Bethesda, Maryland Charles G. Edmonds Richland, Washington lvor A. S. Lewis London, England Marvin L. Vestal Houston, Texas v Contents 1. Introduction . . . . . . . . . . . . . . . . . . . . . . . . . 1 . . . . . . . . . . . 2. Direct Liquid Introduction Interfaces . . . . . . . . . . 5 . . . 2. 1. Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 2. 2. Operating Principles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 2. 3. Specific DLI Interfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 2. 3. 1. Capillary Inlets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 2. 3. 2. Diaphragm Interfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 2. 3. 3. Nebulizing Interfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Practical Liquid Chromatography

Practical Liquid Chromatography Book
Author : S. Perry
Publisher : Springer
Release : 2012-03-17
ISBN : 9781468419375
Language : En, Es, Fr & De

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Book Description :

This book is intended to provide a practical introduction to high-speed, high-efficiency liquid chromatography. It covers modern column technology (which has leapt into prominence only in the last five years) and relates this to the well-established thin-layer techniques. The development of liquid chromatography has proceeded in fits and starts over many years and in alliance with various scientific disciplines. Liquid chromatography has for years fulfilled an effective role in various fields. Ion-exchange chromatography, for example, is particularly associated with the separation of the rare earths, and exclusion chromatography with the fractionation of naturally occurring materials like proteins and of syn thetic polymers. Partition chromatography, especially in the form of paper chromatography, has been an indispensable tool in the study of biochemical systems, while its more recent adsorption counterpart, thin-layer chromatog raphy, developed most rapidly within the pharmacognosic and pharma ceutical fields. Until recently, however, liquid chromatography has not played a prominent role in the field of industrial organic analysis.

Ultra High Performance Liquid Chromatography and Its Applications

Ultra High Performance Liquid Chromatography and Its Applications Book
Author : Q. Alan Xu
Publisher : John Wiley & Sons
Release : 2013-04-01
ISBN : 1118533984
Language : En, Es, Fr & De

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Book Description :

Explores both the benefits and limitations of new UHPLCtechnology High performance liquid chromatography (HPLC) has been widelyused in analytical chemistry and biochemistry to separate,identify, and quantify compounds for decades. The science of liquidchromatography, however, was revolutionized a few years ago withthe advent of ultra-high performance liquid chromatography (UHPLC),which made it possible for researchers to analyze sample compoundswith greater speed, resolution, and sensitivity. Ultra-High Performance Liquid Chromatography and ItsApplications enables readers to maximize the performance ofUHPLC as well as develop UHPLC methods tailored to their particularresearch needs. Readers familiar with HPLC methods will learn howto transfer these methods to a UHPLC platform and vice versa. Inaddition, the book explores a variety of UHPLC applicationsdesigned to support research in such fields as pharmaceuticals,food safety, clinical medicine, and environmental science. The book begins with discussions of UHPLC method development andmethod transfer between HPLC and UHPLC platforms. It then examinespractical aspects of UHPLC. Next, the book covers: Coupling UHPLC with mass spectrometry Potential of shell particles in fast liquid chromatography Determination of abused drugs in human biological matrices Analyses of isoflavones and flavonoids Therapeutic protein characterization Analysis of illicit drugs The final chapter of the book explores the use of UHPLC in drugmetabolism and pharmacokinetics studies for traditional Chinesemedicine. With its frank discussions of UHPLC's benefits and limitations,Ultra-High Performance Liquid Chromatography and ItsApplications equips analytical scientists with the skills andknowledge needed to take full advantage of this new separationtechnology.

High Performance Liquid Chromatography

High Performance Liquid Chromatography Book
Author : W.J. Lough,I.W. Wainer
Publisher : CRC Press
Release : 1995-09-30
ISBN : 9780751400762
Language : En, Es, Fr & De

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Book Description :

High performance liquid chromatography (HPLC) has long been recognized as one of the most useful and versatile analytical techniques. It has now progressed from being a highly expensive method of analysis to a routine technique with wide applications. Consequently there is a requirement in many chemistry and chemistry-related courses for students to acquire a detailed understanding of the principles and practice of HPLC. Written in a manner suitable for undergraduate students studying analytical chemistry and learning about chromatographic analytical techniques applied to pharmaceutical analysis, biochemistry and related disciplines, High-performance Liquid Chromatography: Fundamental Principles and Practice introduces the fundamentals of HPLC. Loosely structured in three parts, the text begins with a thorough introduction of the subject and then progresses through the essential knowledge of the instrumentation needed for HPLC. The final part covers with the applications of HPLC in real-world situations. Developed by a team of international experts from a wide cross-section of disciplines, the text is relevant to a wide range of courses.

Liquid Chromatography

Liquid Chromatography Book
Author : Wilfried M.A. Niessen
Publisher : CRC Press
Release : 1998-09-01
ISBN : 9780824719364
Language : En, Es, Fr & De

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Book Description :

This volume comprehensively relates developments, principles, and applications of combined liquid chromatography-mass spectrometry and other techniques such as capillary electrophoresis and supercritical fluid chromatography combined with mass spectrometry. It covers historical developments, currently important interfaces and technologies, and LC-MS applications in environmental analysis, pharmaceuticals and bioanalysis, and additional fields. It offers in-depth coverage of interfaces and technologies currently important in the laboratory, especially electrospray and APCI, contains an expanded applications section, and provides over 2200 references, tables, equations, and drawings.