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Introduction to Modern Liquid Chromatography

Introduction to Modern Liquid Chromatography Book
Author : Lloyd R. Snyder,Joseph J. Kirkland,John W. Dolan
Publisher : John Wiley & Sons
Release : 2011-09-20
ISBN : 1118210395
Language : En, Es, Fr & De

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Book Description :

The latest edition of the authoritative reference to HPLC High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. This carefully considered Third Edition maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics as: The basis of HPLC separation and the general effects of different experimental conditions Equipment and detection The column—the "heart" of the HPLC system Reversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniques Computer simulation, qualitative and quantitative analysis, and method validation and quality control The separation of large molecules, including both biological and synthetic polymers Chiral separations, preparative separations, and sample preparation Systematic development of HPLC separations—new to this edition Troubleshooting tricks, techniques, and case studies for both equipment and chromatograms Designed to fulfill the needs of the full range of HPLC users, from novices to experts, Introduction to Modern Liquid Chromatography, Third Edition offers the most up-to-date, comprehensive, and accessible survey of HPLC methods and applications available.

Liquid Chromatography

Liquid Chromatography Book
Author : Salvatore Fanali,Paul R. Haddad,Colin Poole,Marja-Liisa Riekkola
Publisher : Elsevier
Release : 2017-06-22
ISBN : 0128093455
Language : En, Es, Fr & De

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Book Description :

Liquid Chromatography: Fundamentals and Instrumentation, Second Edition, is a single source of authoritative information on all aspects of the practice of modern liquid chromatography. It gives those working in both academia and industry the opportunity to learn, refresh, and deepen their understanding of new fundamentals and instrumentation techniques in the field. In the years since the first edition was published, thousands of papers have been released on new achievements in liquid chromatography, including the development of new stationary phases, improvement of instrumentation, development of theory, and new applications in biomedicine, metabolomics, proteomics, foodomics, pharmaceuticals, and more. This second edition addresses these new developments with updated chapters from the most expert researchers in the field. Emphasizes the integration of chromatographic methods and sample preparation Explains how liquid chromatography is used in different industrial sectors Covers the most interesting and valuable applications in different fields, e.g., proteomic, metabolomics, foodomics, pollutants and contaminants, and drug analysis (forensic, toxicological, pharmaceutical, biomedical) Includes references and tables with commonly used data to facilitate research, practical work, comparison of results, and decision-making

Protein Liquid Chromatography

Protein Liquid Chromatography Book
Author : M. Kastner
Publisher : Elsevier
Release : 1999-12-14
ISBN : 0080929249
Language : En, Es, Fr & De

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Book Description :

Protein Liquid Chromatography is a handbook-style guide to liquid chromatography as a tool for isolating and purifying proteins, consisting of 25 individual chapters divided into three parts: Part A covers commonly-used, classic modes of chromatography such as ion-exchange, size-exclusion, and reversed-phase; Part B deals with various target protein classes such as membrane proteins, recombinant proteins, and glycoproteins; and Part C looks at various miscellaneous related topics, including coupling reaction, buffer solution additives, and software. The text as a whole can be viewed as a systematic survey of available methods and how best to use them, but also attempts to provide an exhaustive coverage of each facet. How to solve a specific problem using a chosen method is the overall essence of the volume. The principle philosophy of this compilation is that practical application is everything; therefore, both classical and modern methods are presented in detail, with examples involving conventional, medium- and high-pressure techniques. Over-exposure to history, concept, and theory has deliberately been avoided. The reader will find a wealth of tips and tricks from users for users, including advice on the advantages and disadvantages of each method. Easy-to-read sections on "Getting started now" and "Where to go from here" attempt to provide hands-on, fool-proof detailed practical procedures with complete and even standard model runs for any scientist or technician at work in this area.

Liquid Chromatography

Liquid Chromatography Book
Author : Salvatore Fanali,Paul R. Haddad,Colin Poole,Marja-Liisa Riekkola
Publisher : Elsevier
Release : 2017-06-23
ISBN : 0128093447
Language : En, Es, Fr & De

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Book Description :

Liquid Chromatography: Applications, Second Edition,is a single source of authoritative information on all aspects of the practice of modern liquid chromatography. It gives those working in both academia and industry the opportunity to learn, refresh, and deepen their knowledge of the wide variety of applications in the field. In the years since the first edition was published, thousands of papers have been released on new achievements in liquid chromatography, including the development of new stationary phases, improvement of instrumentation, development of theory, and new applications in biomedicine, metabolomics, proteomics, foodomics, pharmaceuticals, and more. This second edition addresses these new developments with updated chapters from the most expert researchers in the field. Emphasizes the integration of chromatographic methods and sample preparation Explains how liquid chromatography is used in different industrial sectors Covers the most interesting and valuable applications in different fields, e.g., proteomic, metabolomics, foodomics, pollutants and contaminants, and drug analysis (forensic, toxicological, pharmaceutical, biomedical) Includes references and tables with commonly used data to facilitate research, practical work, comparison of results, and decision-making

High Performance Liquid Chromatography in Phytochemical Analysis

High Performance Liquid Chromatography in Phytochemical Analysis Book
Author : Monika Waksmundzka-Hajnos,Joseph Sherma
Publisher : CRC Press
Release : 2010-11-08
ISBN : 9781420092615
Language : En, Es, Fr & De

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Book Description :

The powerful, efficient technique of high performance liquid chromatography (HPLC) is essential to the standardization of plant-based drugs, identification of plant material, and creation of new herbal medicines. Filling the void in this critical area, High Performance Liquid Chromatography in Phytochemical Analysis is the first book to give a comp

Liquid Chromatography

Liquid Chromatography Book
Author : M.D. Marazuela,S. Bogialli
Publisher : Elsevier Inc. Chapters
Release : 2013-01-08
ISBN : 0128071222
Language : En, Es, Fr & De

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Book Description :

Food safety has emerged as a global issue with public health and international trade implications. Food safety programs include the control of veterinary residues, posed by the use of banned and authorized substances in animal food production. Veterinary drugs play a significant role, since they may be harmful for human health if exposure is not controlled. Nowadays, food safety laboratories face a competitive market, where government regulations mandate increase sample testing. In particular, it is necessary to develop and validate analytical methods to monitor drug residues in animal-derived food that allow the simultaneous analysis of a vast number of residues, reducing analytical costs and increasing sample throughput. Current analytical methods for confirmatory analysis and multiresidue screening are generally based on liquid chromatography–mass spectrometry (LC–MS). This chapter addresses the contribution of different LC–MS techniques to the field of veterinary drug analysis in food safety, with particular emphasis On their possibilities, limitations, and related applications.

Liquid Chromatography

Liquid Chromatography Book
Author : B. Paull,P.N. Nesterenko
Publisher : Elsevier Inc. Chapters
Release : 2013-01-08
ISBN : 0128071354
Language : En, Es, Fr & De

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Book Description :

The basic principles and fundamentals of modern ion chromatography are presented with focus on a key separation mechanism, including ion exchange, ion exclusion, chelation, and zwitterionic interactions. The most common types of ion chromatographic columns, eluents, and detection modes are briefly discussed, together with possible applications of ion chromatography for the determination of inorganic and organic anions and cations.

High Performance Liquid Chromatography Capillary Electrophoresis

High Performance Liquid Chromatography   Capillary Electrophoresis Book
Author : Andrea Weston,Phyllis R. Brown
Publisher : Elsevier
Release : 1997-07-21
ISBN : 9780080534169
Language : En, Es, Fr & De

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Book Description :

HPLC and CE: Principles and Practice presents the latest information on the most powerful separation techniques available: high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE). Fundamental theory, instrumentation, modes of operation, and optimization of separations are presented in a concise, non-technical style to help the user in choosing the appropriate technique quickly and accurately. Well- illustrated and containing convenientend-of-chapter summaries of the major concepts, the book provides in-depth coverage of trouble-shooting, improvement of resolution, data manipulation, selectivity, and sensitivity. Graduate students, technicians, and researchers who must use separations with little or no background in analytical chemistry can overcome separation anxiety and get started in obtaining the best possible separations in minimal time. The book will alsobe useful to analytical chemists who need a better understanding of theory and processes. Fully up-to-date information on both HPLC and CE includes troubleshooting and comparisons of the two techniques Applicable to a wide variety of separation problems Covers basic concepts governing any separation as well as instrumentation and how to use it Helps the user to obtain optimal resolution in minimal time Contains information on special procedures such as chiral separations, affinity chromatography, and sample preparation Includes information on upcoming trends such as miniaturization Major concepts in each chapter are organized to allow access to information easily and quickly Contains practical bibliography for accessing the literature

Polymer Characterization by Liquid Chromatography

Polymer Characterization by Liquid Chromatography Book
Author : G. Glöckner
Publisher : Elsevier
Release : 1987-02-01
ISBN : 9780080858371
Language : En, Es, Fr & De

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Book Description :

The main subject of this book is the characterization of plastics. To a high degree the properties of these polymers depend on the distribution of the molar mass and of other structural features, and small deviations frequently have a great effect. Therefore the characterization of polymers cannot be restricted to the determination of mean values but must yield information on these distributions. Using classical methods, the analytical fractionation of polymer homologues and structurally isomeric polymers is extremely time-consuming. Therefore, efficient chromatographic techniques are being increasingly employed in modern polymer characterization. In the first place, high-performance liquid chromatography is applied, in the form of size exclusion chromatography. It is also possible, however, to use other separation modes. More space is devoted to these other possibilities in this volume than is merited by their current range of applications, as the author believes that many separation problems will be solved by separation techniques of the non-exclusion type. Nevertheless, much emphasis is placed on size exclusion chromatography. Not only because of its current wide range of applications, but also because its relative importance, as a complement to other chromatographic techniques may even increase in the forthcoming years. This book is the first to cover all phenomena related to the above considerations. Starting with an introduction to basic liquid chromatography and to polymer science, it deals with the adsorption behaviour of polymers, with gradient techniques, with the kinetic band broadening in liquid chromatography, with instrumental features and packing materials. The book consists of four balanced sections and related information from about 1800 references is compiled in the tables. Some 250 figures and 30 tables will help give the reader a clear insight of the topics discussed. The book is aimed at helping the analyst or polymer chemist who is looking for information about chromatographic methods for the characterization of polymers.

High Performance Liquid Chromatography

High Performance Liquid Chromatography Book
Author : Csaba Horváth
Publisher : Elsevier
Release : 2013-10-22
ISBN : 1483262022
Language : En, Es, Fr & De

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Book Description :

High-Performance Liquid Chromatography: Advances and Perspectives, Volume 1 deals with the fundamental aspects of high-performance liquid chromatography, a technique used in chemical analysis. The publication provides accounts, presented by experts in the field, of a variety of topics in high-performance liquid chromatography. Each chapter covers interesting subjects such as the evolution of liquid chromatography; the use of bonded phases in high-performance chromatography; effects of ionization and complex formation on retention and selectivity in reversed-phase chromatography; and gradient elution. Chromatographers, chemists, and researchers in the field of chemical analysis will find this book a valuable reference material.

High Performance Liquid Chromatography

High Performance Liquid Chromatography Book
Author : Mary T. Gilbert
Publisher : Elsevier
Release : 2013-10-22
ISBN : 1483281299
Language : En, Es, Fr & De

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Book Description :

High Performance Liquid Chromatography focuses on the developments, operating techniques, practices, equipment, and packing materials involved in High Performance Liquid Chromatography (HPLC). The book first offers information on basic chromatographic theory, equipment, and the column. Topics include resolution, efficiency, pumps and gradient systems, connectors, detectors, injectors, column packing and testing, packing materials, and coupling of columns. The text also ponders on sample treatment and separation methods, as well as trace analysis, reversed phase chromatography, and selection/optimization conditions. The publication examines adjustment of selectivity by the use of eluent additives and preparative liquid chromatography. Discussions focus on chromatography on dynamically modified oxide gels, metal complexation, crown ethers, ion pair chromatography, materials for preparative chromatography, and separation strategy. The text also reviews the trends in the practice of HPLC and chiral chromatography. The book is a dependable reference for readers interested in High Performance Liquid Chromatography.

Basic Liquid Chromatography

Basic Liquid Chromatography Book
Author : Nina Hadden
Publisher : Unknown
Release : 1971
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Basic Liquid Chromatography book written by Nina Hadden, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Liquid Chromatography Mass Spectrometry

Liquid Chromatography Mass Spectrometry Book
Author : Wilfried M.A. Niessen
Publisher : CRC Press
Release : 2006-08-09
ISBN : 1420014544
Language : En, Es, Fr & De

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Book Description :

A constructive evaluation of the most significant developments in liquid chromatography-mass spectrometry (LC-MS) and its uses for quantitative bioanalysis and characterization for a diverse range of disciplines, Liquid Chromatography-Mass Spectrometry, Third Edition offers a well-rounded coverage of the latest technological developments and

Trace Analysis with Microcolumn Liquid Chromatography

Trace Analysis with Microcolumn Liquid Chromatography Book
Author : Milos Krejci
Publisher : CRC Press
Release : 1992-04-15
ISBN : 9780824786410
Language : En, Es, Fr & De

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Book Description :

This book provides a detailed description of technical elements of a microbore column liquid chromatograph suitable for use in trace analysis. It presents examples of analyses, especially from the spheres of biochemistry, pharmacology, and environmental analytical chemistry.

Modern Size Exclusion Liquid Chromatography

Modern Size Exclusion Liquid Chromatography Book
Author : W. W. Yau,Wallace Wen-Chuan Yau,Joseph J. Kirkland,Joseph Jack Kirkland,Donald D. Bly,Donald D.. Bly
Publisher : Wiley-Interscience
Release : 1979-09-27
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

A critical treatment of all aspects, containing definitive examples, detailed description of data handling, compilations of information on columns and column packings, and tables of important solvents and standard reference materials. Combines a basic, unified theory with practical know-how, integrating methods of gel permeation and gel filtration chromatography. Provides an understanding of molecular characterization problems as well as a background for developing new and improved characterization methods and apparatus for HPSEC performed with columns of small particles at high pressures.

High speed Liquid Chromatography

High speed Liquid Chromatography Book
Author : Peter M. Rajcsanyi,Elisabeth Rajcsanyi
Publisher : Unknown
Release : 1975
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download High speed Liquid Chromatography book written by Peter M. Rajcsanyi,Elisabeth Rajcsanyi, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

High Performance Liquid Chromatography HPLC

High Performance Liquid Chromatography  HPLC  Book
Author : Yuegang Zuo
Publisher : Nova Science Pub Incorporated
Release : 2014
ISBN : 9781629488547
Language : En, Es, Fr & De

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Book Description :

High-performance liquid chromatography (HPLC) has emerged as the most powerful and versatile separation and analytical method. This book covers not only the conventional HPLC techniques but also the new developments, novel separation modes, column technology, as well as procedures and practices, particularly the advanced applications of HPLC in the fields of pharmaceutical, clinical, bioanalytical and food sciences.

Separation of Fullerenes by Liquid Chromatography

Separation of Fullerenes by Liquid Chromatography Book
Author : Kiyokatsu Jinno
Publisher : Unknown
Release : 1999
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Since 1970, when their existence was first predicted, fullerenes have attracted the attention of scientists in a wide variety of scientific fields. Numerous different compounds are needed to evaluate and estimate their chemical and physical properties, thus the isolation and purification of fullerenes and their derivatives from carbon soot mixtures is a key process. Separation of Fullerenes contains the most up-to-date innovations from the world's leaders in their rapidly developing field. Uniquely devoted to separation techniques, this book describes the different stationary phases currently used which can isolate fullerenes by liquid chromatography, and the challenges of finding or developing new stationary phases. Also discussed is the extension of the separation technique to large scale preparations, enabling scientists to obtain considerable quantities of fullerenes from one chromatographic run. The use of fullerenes as the stationary phase is also described. Covering C60, C70 and higher than C70 fullerenes, this book will be welcomed by graduates and researchers in many areas of chemistry.