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Laboratory Methods in Microfluidics

Laboratory Methods in Microfluidics Book
Author : Basant Giri
Publisher : Elsevier
Release : 2017-06-01
ISBN : 9780128132357
Language : En, Es, Fr & De

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Book Description :

Laboratory Methods in Microfluidics features a range of lab methods and techniques necessary to fully understanding microfluidic technology applications. Microfluidics deals with the manipulation of small volume of fluids at sub-millimeter scale domain channels. This exciting new field is becoming an increasingly popular subject both for research and education in various disciplines of science, including chemistry, chemical engineering, and environmental science. The unique properties of microfluidic technologies, such as rapid sample processing and precise control of fluids in assay, have made them attractive candidates to replace traditional experimental approaches. Practical for students, instructors, and researchers, Laboratory Methods in Microfluidics provides a much-needed, comprehensive new laboratory reference in this rapidly growing and exciting new field of research. Provides a number of detailed methods and instructions for experiments in microfluidics Features an appendix that highlights several standard laboratory techniques, including reagent preparation plus a list of materials vendors for quick reference Authored by a microfluidics expert with nearly a decade of research on the subject

Laboratory Methods in Microfluidics

Laboratory Methods in Microfluidics Book
Author : Basant Giri
Publisher : Elsevier
Release : 2017-05-15
ISBN : 0128132361
Language : En, Es, Fr & De

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Book Description :

Laboratory Methods in Microfluidics features a range of lab methods and techniques necessary to fully understand microfluidic technology applications. Microfluidics deals with the manipulation of small volumes of fluids at sub-millimeter scale domain channels. This exciting new field is becoming an increasingly popular subject both for research and education in various disciplines of science, including chemistry, chemical engineering and environmental science. The unique properties of microfluidic technologies, such as rapid sample processing and precise control of fluids in assay have made them attractive candidates to replace traditional experimental approaches. Practical for students, instructors, and researchers, this book provides a much-needed, comprehensive new laboratory reference in this rapidly growing and exciting new field of research. Provides a number of detailed methods and instructions for experiments in microfluidics Features an appendix that highlights several standard laboratory techniques, including reagent preparation plus a list of materials vendors for quick reference Authored by a microfluidics expert with nearly a decade of research on the subject

Microfluidic Techniques

Microfluidic Techniques Book
Author : Shelley D. Minteer
Publisher : Springer Science & Business Media
Release : 2006
ISBN : 1592599974
Language : En, Es, Fr & De

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Book Description :

This volume highlights recent important advances in microfluidic techniques for biological applications. Opening chapters focus on the most popular techniques for fabrication of microchips (photolithography, laser ablation, and soft lithography), while further sections focus on microfluidic techniques for bioanalytical assays and bioprocesses, such as DNA analysis, PCR, immunoassays, and cell reactors. Microfluidic Techniques will provide molecular biologists and biochemists with the state-of-the-art technical information required to perform microscale bioassays and bioprocessing in the laboratory.

Laboratory Methods in Dynamic Electroanalysis

Laboratory Methods in Dynamic Electroanalysis Book
Author : M. Teresa Fernández Abedul
Publisher : Elsevier
Release : 2019-10-13
ISBN : 0128159332
Language : En, Es, Fr & De

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Book Description :

Laboratory Methods in Dynamic Electroanalysis is a useful guide to introduce analytical chemists and scientists of related disciplines to the world of dynamic electroanalysis using simple and low-cost methods. The trend toward decentralization of analysis has made this fascinating field one of the fastest-growing branches of analytical chemistry. As electroanalytical devices have moved from conventional electrochemical cells (10-20 mL) to current cells (e.g. 5-50 mL) based on different materials such as paper or polymers that integrate thick- or thin-film electrodes, interesting strategies have emerged, such as the combination of microfluidic cells and biosensing or nanostructuration of electrodes. This book provides detailed, easy procedures for dynamic electroanalysis and covers the main trends in electrochemical cells and electrodes, including microfluidic electrodes, electrochemical detection in microchip electrophoresis, nanostructuration of electrodes, development of bio (enzymatic, immuno, and DNA) assays, paper-based electrodes, interdigitated array electrodes, multiplexed analysis, and combination with optics. Different strategies and techniques (amperometric, voltammetric, and impedimetric) are presented in a didactic, practice-based way, and a bibliography provides readers with additional sources of information. Provides easy-to-implement experiments using low-cost, simple equipment Includes laboratory methodologies that utilize both conventional designs and the latest trends in dynamic electroanalysis Goes beyond the fundamentals covered in other books, focusing instead on practical applications of electroanalysis

Microfluidics and Lab on a Chip

Microfluidics and Lab on a Chip Book
Author : Andreas Manz,Giuseppina Simone,Jonathan S. O'Connor,Pavel Neuzil
Publisher : Royal Society of Chemistry
Release : 2020-09-24
ISBN : 1782628339
Language : En, Es, Fr & De

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Book Description :

Responding to the need for an affordable, easy-to-read textbook that introduces microfluidics to undergraduate and postgraduate students, this concise book will provide a broad overview of the important theoretical and practical aspects of microfluidics and lab-on-a-chip, as well as its applications.

Microfluidic Electrophoresis

Microfluidic Electrophoresis Book
Author : Debashis Dutta
Publisher : Humana Press
Release : 2018-11-29
ISBN : 9781493989638
Language : En, Es, Fr & De

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Book Description :

This detailed book provides a set of protocols necessary for the development of a variety of microchip-based electrophoretic assays. It compiles a range of such electrophoretic methods by leading researchers in the field, covering subjects such as microfluidic device fabrication, on-chip sample preparation, theoretical/simulation protocols for assessing these separation methods, as well as common practices followed when applying them to important real world applications. The contents of the book range from protocols for classical assays to those involving pioneering separation techniques recently developed by the scientific community for advancing our analytical capabilities. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Microfluidic Electrophoresis: Methods and Protocols serves as an convenient text for academic researchers as well as practicing engineers, biochemists, and analytical laboratory professionals.

Lab on a Chip Devices and Micro Total Analysis Systems

Lab on a Chip Devices and Micro Total Analysis Systems Book
Author : Jaime Castillo-León,Winnie E. Svendsen
Publisher : Springer
Release : 2014-11-05
ISBN : 3319086871
Language : En, Es, Fr & De

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Book Description :

This book covers all the steps in order to fabricate a lab-on-a-chip device starting from the idea, the design, simulation, fabrication and final evaluation. Additionally, it includes basic theory on microfluidics essential to understand how fluids behave at such reduced scale. Examples of successful histories of lab-on-a-chip systems that made an impact in fields like biomedicine and life sciences are also provided. This book also: · Provides readers with a unique approach and toolset for lab-on-a-chip development in terms of materials, fabrication techniques, and components · Discusses novel materials and techniques, such as paper-based devices and synthesis of chemical compounds on-chip · Covers the four key aspects of development: basic theory, design, fabrication, and testing · Provides readers with a comprehensive list of the most important journals, blogs, forums, and conferences where microfluidics and lab-on-a-chip news, methods, techniques and challenges are presented and discussed, as well as a list of companies providing design and simulation support, components, and/or developing lab-on-a-chip and microfluidic devices.

Electrokinetics in Microfluidics

Electrokinetics in Microfluidics Book
Author : Dongqing Li
Publisher : Elsevier
Release : 2004-08-20
ISBN : 9780080530741
Language : En, Es, Fr & De

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Book Description :

A lab-on-a-chip device is a microscale laboratory on a credit-card sized glass or plastic chip with a network of microchannels, electrodes, sensors and electronic circuits. These labs on a chip can duplicate the specialized functions as performed by their room-sized counterparts, such as clinical diagnoses, PCR and electrophoretic separation. The advantages of these labs on a chip include significant reduction in the amounts of samples and reagents, very short reaction and analysis time, high throughput and portability. Generally, a lab-on-a-chip device must perform a number of microfluidic functions: pumping, mixing, thermal cycling/incubating, dispensing, and separating. Precise manipulation of these microfluidic processes is key to the operation and performance of labs on a chip. The objective of this book is to provide a fundamental understanding of the interfacial electrokinetic phenomena in several key microfluidic processes, and to show how these phenomena can be utilised to control the microfluidic processes. For this purpose, this book emphasises the theoretical modelling and the numerical simulation of these electrokinetic phenomena in microfluidics. However, experimental studies of the electrokinetic microfluidic processes are also highlighted in sufficient detail. The first book which systematically reviews electrokinetic microfluidics processes for lab-on-a chip applications Covers modelling and numerical simulation of the electrokinetic microfluidics processes Providing information on experimental studies and details of experimental techniques, which are essential for those who are new to this field

Methods in Bioengineering

Methods in Bioengineering Book
Author : Jeffrey D. Zahn
Publisher : Artech House
Release : 2009-10
ISBN : 1596934018
Language : En, Es, Fr & De

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Book Description :

This unique volume presents leading-edge microfluidics methods used to handle, manipulate, and analyze cells, particles, and biological components (e.g., proteins and DNA) for microdiagnostics. The authors offer clear and detailed guidance on microfabrication techniques utilized to create microfluidic devices and on-chip flow control and mixing Microsystems, protein and DNA handling devices for electrophoretic and isoelectric separations in microchromatography columns, microfluidic manipulations of droplets via electrowetting and particles via dielectrophoresis for separations and chemical reactions, integrated optical characterization of microfluidic devices, controlling chemical gradients within devices, microimmunoassay diagnostics, multiphase microfluidics used in droplet formation for controlled chemical reactions, particle separation and analysis in Micro-FACS systems, flow characterization techniques in microfluidic devices and patterning and utilizing cytoskeletal filaments and cellular transport protein within microstructures.

Microfluidics for Single Cell Analysis

Microfluidics for Single Cell Analysis Book
Author : Jin-Ming Lin
Publisher : Springer Nature
Release : 2019-08-28
ISBN : 9813297298
Language : En, Es, Fr & De

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Book Description :

This book summarizes the various microfluidic-based approaches for single-cell capture, isolation, manipulation, culture and observation, lysis, and analysis. Single-cell analysis reveals the heterogeneities in morphology, functions, composition, and genetic performance of seemingly identical cells, and advances in single-cell analysis can overcome the difficulties arising due to cell heterogeneity in the diagnostics for a targeted model of disease. This book provides a detailed review of the state-of-the-art techniques presenting the pros and cons of each of these methods. It also offers lessons learned and tips from front-line investigators to help researchers overcome bottlenecks in their own studies. Highlighting a number of techniques, such as microfluidic droplet techniques, combined microfluidics-mass-spectrometry systems, and nanochannel sampling, it describes in detail a new microfluidic chip-based live single-cell extractor (LSCE) developed in the editor’s laboratory, which opens up new avenues to use open microfluidics in single-cell extraction, single-cell mass spectrometric analysis, single-cell adhesion analysis and subcellular operations. Serving as both an elementary introduction and advanced guidebook, this book interests and inspires scholars and students who are currently studying or wish to study microfluidics-based cell analysis methods.

Microfluidics and Lab on a chip

Microfluidics and Lab on a chip Book
Author : Andreas Manz,Pavel Neužil,Jonathan S O'Connor,Giuseppina Simone
Publisher : Royal Society of Chemistry
Release : 2020-09-24
ISBN : 1788019385
Language : En, Es, Fr & De

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Book Description :

Microfluidic technology is revolutionising a number of scientific fields, including chemistry, biology, diagnostics, and engineering. The ability to manipulate fluids and objects within networks of micrometre-scale channels allows reductions in processing and analysis times, reagent and sample consumption, and waste production, whilst allowing fine control and monitoring of chemical or biological processes. The integration of multiple components and processes enable “lab-on-a-chip” devices and “micro total analysis systems” that have applications ranging from analytical chemistry, organic synthesis, and clinical diagnostics to cell biology and tissue engineering. This concise, easy-to-read book is perfectly suited for instructing newcomers on the most relevant and important aspects of this exciting and dynamic field, particularly undergraduate and postgraduate students embarking on new studies, or for those simply interested in learning about this widely applicable technology. Written by a team with more than 20 years of experience in microfluidics research and teaching, the book covers a range of topics and techniques including fundamentals (e.g. scaling laws and flow effects), microfabrication and materials, standard operations (e.g. flow control, detection methods) and applications. Furthermore, it includes questions and answers that provide for the needs of students and teachers in the area.

Microfluidic Devices for Biomedical Applications

Microfluidic Devices for Biomedical Applications Book
Author : Xiujun James Li,Yu Zhou
Publisher : Elsevier
Release : 2013-10-31
ISBN : 0857097040
Language : En, Es, Fr & De

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Book Description :

Microfluidics or lab-on-a-chip (LOC) is an important technology suitable for numerous applications from drug delivery to tissue engineering. Microfluidic devices for biomedical applications discusses the fundamentals of microfluidics and explores in detail a wide range of medical applications. The first part of the book reviews the fundamentals of microfluidic technologies for biomedical applications with chapters focussing on the materials and methods for microfabrication, microfluidic actuation mechanisms and digital microfluidic technologies. Chapters in part two examine applications in drug discovery and controlled-delivery including micro needles. Part three considers applications of microfluidic devices in cellular analysis and manipulation, tissue engineering and their role in developing tissue scaffolds and stem cell engineering. The final part of the book covers the applications of microfluidic devices in diagnostic sensing, including genetic analysis, low-cost bioassays, viral detection, and radio chemical synthesis. Microfluidic devices for biomedical applications is an essential reference for medical device manufacturers, scientists and researchers concerned with microfluidics in the field of biomedical applications and life-science industries. Discusses the fundamentals of microfluidics or lab-on-a-chip (LOC) and explores in detail a wide range of medical applications Considers materials and methods for microfabrication, microfluidic actuation mechanisms and digital microfluidic technologies Considers applications of microfluidic devices in cellular analysis and manipulation, tissue engineering and their role in developing tissue scaffolds and stem cell engineering

Microfluidics

Microfluidics Book
Author : Yujun Song,Daojian Cheng,Liang Zhao
Publisher : John Wiley & Sons
Release : 2018-05-07
ISBN : 3527341064
Language : En, Es, Fr & De

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Book Description :

The first book offering a global overview of fundamental microfluidics and the wide range of possible applications, for example, in chemistry, biology, and biomedical science. As such, it summarizes recent progress in microfluidics, including its origin and development, the theoretical fundamentals, and fabrication techniques for microfluidic devices. The book also comprehensively covers the fluid mechanics, physics and chemistry as well as applications in such different fields as detection and synthesis of inorganic and organic materials. A useful reference for non-specialists and a basic guideline for research scientists and technicians already active in this field or intending to work in microfluidics.

Microfluidics for Medical Applications

Microfluidics for Medical Applications Book
Author : Albert van den Berg,Loes Segerink
Publisher : Royal Society of Chemistry
Release : 2014-11-19
ISBN : 1849737592
Language : En, Es, Fr & De

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Book Description :

Lab-on-a-chip devices for point of care diagnostics have been present in clinics for several years now. Alongside their continual development, research is underway to bring the organs and tissue on-a-chip to the patient, amongst other medical applications of microfluidics. This book provides the reader with a comprehensive review of the latest developments in the application of microfluidics to medicine and is divided into three main sections. The first part of the book discusses the state-of-the-art in organs and tissue on a chip; the second provides a thorough background to microfluidics for medicine, and the third (and largest) section provides numerous examples of point-of-care diagnostics. Written with students and practitioners in mind, and with contributions from the leaders in the field across the globe, this book provides a complete digest of the state-of-the-art in microfluidics medical devices and will provide a handy resource for any laboratory or clinic involved in the development or application of such devices.

Nanofluidics and Microfluidics

Nanofluidics and Microfluidics Book
Author : Shaurya Prakash,Junghoon Yeom
Publisher : William Andrew
Release : 2014-01-16
ISBN : 1437744702
Language : En, Es, Fr & De

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Book Description :

To provide an interdisciplinary readership with the necessary toolkit to work with micro- and nanofluidics, this book provides basic theory, fundamentals of microfabrication, advanced fabrication methods, device characterization methods and detailed examples of applications of nanofluidics devices and systems. Case studies describing fabrication of complex micro- and nanoscale systems help the reader gain a practical understanding of developing and fabricating such systems. The resulting work covers the fundamentals, processes and applied challenges of functional engineered nanofluidic systems for a variety of different applications, including discussions of lab-on-chip, bio-related applications and emerging technologies for energy and environmental engineering. The fundamentals of micro- and nanofluidic systems and micro- and nanofabrication techniques provide readers from a variety of academic backgrounds with the understanding required to develop new systems and applications. Case studies introduce and illustrate state-of-the-art applications across areas, including lab-on-chip, energy and bio-based applications. Prakash and Yeom provide readers with an essential toolkit to take micro- and nanofluidic applications out of the research lab and into commercial and laboratory applications.

Fundamentals of Microfluidics and Lab on a Chip for Biological Analysis and Discovery

Fundamentals of Microfluidics and Lab on a Chip for Biological Analysis and Discovery Book
Author : Paul C.H. Li
Publisher : CRC Press
Release : 2010-02-24
ISBN : 1439882177
Language : En, Es, Fr & De

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Book Description :

Lab-on-a-chip technology permits us to make many important discoveries that can only be observed at the microscale or the nanoscale. Using this technology, biological and biochemical analyses translate into greater sensitivity, more accurate results, and more valuable findings. Authored by one of the field’s pioneering researchers, Fundamentals of Microfluidics and Lab on a Chip for Biological Analysis and Discovery focuses on all key aspects of microfluidic lab-on-a-chip technologies to offer an exceptionally cohesive overview of the science, its limitations, breakthroughs made over the years, and currently emerging advances. The book emphasizes analytical applications of microfluidic technology and offers in-depth coverage of micromachining methods, microfluidic operations, chemical separations, sample preparation and injection methods, detection technology, and various chemical and biological analyses. Other topics of interest include the use of polymeric chips, fluid flow valve and control, single-cell analysis, DNA and RNA amplification techniques, DNA hybridization, immunoassays, and enzymatic assays. The book includes more than 300 figures that depict novel chip functions and breakthroughs and 16 tables summarize materials and refer readers to additional resources. An appendix compiles extensive analytical applications from emerging and established research groups. Beginners in the field will find the book useful for navigating the vast literature related to the technology, while experienced researchers will rely on the compiled information for easy comparison and references for further study. Derived from the highly popular Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery (2006), this volume is also readily adaptable for classroom use. Problem sets in each chapter help students test their assimilation of the material and clarify challenging concepts. The book contains a comprehensive glossary, a complete index, and extensive references. A solutions manual is available with qualifying course adoption.

Lab on a chip

Lab on a chip Book
Author : Albert Berg
Publisher : Elsevier
Release : 2003
ISBN : 0444511008
Language : En, Es, Fr & De

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Book Description :

In the past ten years there has been a rapid growth of the research and application area known as Lab-on-a-Chip. After an initial focus on electrokinetic separation techniques on chip, the scope of the field has widened to include topics like microfluidics, DNA analysis, cell analysis, microreactors and mass spectrometer interfacing. As well as the analytical chemistry community, synthetic chemists, chemical engineers, biochemists and biomedical engineers are now also becoming more and more interested in using new micro- and nanotechnological techniques. This first Lab-on-a-Chip book contains a broad collection of papers on microtechnology, microfluidics, analytical methods and applications. All contributions are written by leading researchers in their respective fields, and provide new scientists with an overview of the field, to make him/her aware of the enormous opportunities offered by modern technology. The work presented in this book will definitely stimulate readers to new ideas and concepts, and lead to further innovations in this area. - Provides a quick introduction into the different aspects of this field - Describes technology that has already revolutionized the world of chemical and biochemical analysis and synthesis - All contributions are written by leading researchers in their respective fields

Microfluidics for Biologists

Microfluidics for Biologists Book
Author : Chandra K. Dixit,Ajeet Kaushik
Publisher : Springer
Release : 2016-10-13
ISBN : 3319400363
Language : En, Es, Fr & De

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Book Description :

This book describes novel microtechnologies and integration strategies for developing a new class of assay systems to retrieve desired health information from patients in real-time. The selection and integration of sensor components and operational parameters for developing point-of-care (POC) are also described in detail. The basics that govern the microfluidic regimen and the techniques and methods currently employed for fabricating microfluidic systems and integrating biosensors are thoroughly covered. This book also describes the application of microfluidics in the field of cell and molecular biology, single cell biology, disease diagnostics, as well as the commercially available systems that have been either introduced or have the potential of being used in research and development. This is an ideal book for aiding biologists in understanding the fundamentals and applications of microfluidics. This book also: Describes the preparatory methods for developing 3-dimensional microfluidic structures and their use for Lab-on-a-Chip design Explains the significance of miniaturization and integration of sensing components to develop wearable sensors for point-of-care (POC) Demonstrates the application of microfluidics to life sciences and analytical chemistry, including disease diagnostics and separations Motivates new ideas related to novel platforms, valving technology, miniaturized transduction methods, and device integration to develop next generation sequencing Discusses future prospects and challenges of the field of microfluidics in the areas of life sciences in general and diagnostics in particular

Diagnostic Devices with Microfluidics

Diagnostic Devices with Microfluidics Book
Author : Francesco Piraino,Šeila Selimović
Publisher : CRC Press
Release : 2017-06-26
ISBN : 1351650483
Language : En, Es, Fr & De

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Book Description :

This book provides a current view of the research and commercial landscape of diagnostics devices, particularly those that utilize microscale technologies, intended for both patient and laboratory use. Common diagnostic devices that are based on microfluidic principles include glucose sensors for diabetic patients and over-the-counter pregnancy tests. Other diagnostic devices are being developed to quickly test a patient for bacterial and viral infections, and other diseases. The chapters, written by experts from around the world, discuss how to fabricate, apply, and market microfluidic diagnostic chips – for lab and at-home use. Most importantly, the book also contains a discussion of topics relevant to the private sector, including patient-focused, market-oriented development of diagnostics devices.

Alternative Methods for Fabricating Lab on a Chip Microfluidic Platforms

Alternative Methods for Fabricating Lab on a Chip Microfluidic Platforms Book
Author : Hari Prasath Shanmugam
Publisher : Unknown
Release : 2007
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Alternative Methods for Fabricating Lab on a Chip Microfluidic Platforms book written by Hari Prasath Shanmugam, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.