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Introductory Experiments On Biomolecules And Their Interactions

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Introductory Experiments on Biomolecules and their Interactions

Introductory Experiments on Biomolecules and their Interactions Book
Author : Robert K. Delong,Qiongqiong Zhou
Publisher : Academic Press
Release : 2015-03-06
ISBN : 0128010126
Language : En, Es, Fr & De

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Book Description :

Introductory Experiments on Biomolecules and their Interactions provides a novel approach to teaching biomolecules in the lab. While featuring the requisite fundamentals, it also captures the author’s experience in industry, thus providing unique, up-to-date experiments which take the learning experience one-step further. The text parallels lectures using a standard biochemistry undergraduate text. Unlike most current lab manuals available in the market which simply emphasize an introduction of techniques, this lab manual provides students with opportunities to demonstrate and prove the knowledge and theories they learn from class. Features quantitative analysis of RNA degradation by RNase Contains problem sets, calculations, and references for each lab fully immersing students in the learning process Includes instruction on how to maintain a lab notebook and write a formal lab report Provides hands-on engagement with the four major types of biomolecules and “real-life and better applied examples of molecular interactions

An Introduction to Single Molecule Biophysics

An Introduction to Single Molecule Biophysics Book
Author : Yuri L. Lyubchenko
Publisher : CRC Press
Release : 2017-11-22
ISBN : 1351333097
Language : En, Es, Fr & De

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Book Description :

This book gives an accessible, detailed overview on techniques of single molecule biophysics (SMB), showing how they are applied to numerous biological problems associated with understanding the molecular mechanisms of DNA replication, transcription, and translation, as well as functioning of molecular machines. It covers major single molecule imaging and probing techniques, highlighting key strengths and limitations of each method using recent examples. The chapters begin with a discussion of single molecule fluorescence techniques followed by an overview of the atomic force microscope and its use for direct time-lapse visualization of dynamics of molecular complexes at the nanoscale, as well as applications in measurements of interactions between molecules and mechanical properties of isolated molecules and their complexes. The next chapters address magnetic tweezers and optical tweezers, including instrumentation, fundamentals of operation, and applications. A final chapter turns to nanopore transport and nanopore-based DNA sequencing technology that will play a major role in next-generation genomics and healthcare applications. Covers the most widely used single molecule biophysics techniques Provides numerous examples of current biological applications Includes practical tips on measurement optimization, resolution limits, etc. Yuri L. Lyubchenko is Professor of Pharmaceutical Sciences at the University of Nebraska Medical Center.

The Smallest Biomolecules Diatomics and their Interactions with Heme Proteins

The Smallest Biomolecules  Diatomics and their Interactions with Heme Proteins Book
Author : Abhik Ghosh
Publisher : Elsevier
Release : 2011-10-13
ISBN : 9780080556321
Language : En, Es, Fr & De

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Book Description :

This is not a book on NO biology, nor about hemoglobin, nor about heme-based sensors per se. Of course, it covers all these topics and more, but above all, it aims at providing a truly multidisciplinary perspective of heme-diatomic interactions. The overarching goal is to build bridges among disciplines, to bring about a meeting of minds. The contributors to this book hail from diverse university departments and disciplines – chemistry, biochemistry, molecular biology, microbiology, zoology, physics, medicine and surgery, bringing with them very different views of heme-diatomic interactions. The hope is that the juxtaposition of this diversity will lead to increased exchanges of ideas, approaches, and techniques across traditional disciplinary boundaries. The authors represent a veritable Who’s Who of heme protein research and include John Olson, Tom Spiro, Walter Zumft, F. Ann Walker, Teizo Kitagawa, W. Robert Scheidt, Pat Farmer, Marie-Alda Gilles-Gonzalez, and many other equally distinguished scientists. Extremely distinguished list of authors Multidisciplinary character – equally suitable for chemists and biochemists Covers the hottest topics in heme protein research: sensors, NO biology, new roles of hemoglobin, etc.

Real Time Analysis of Biomolecular Interactions

Real Time Analysis of Biomolecular Interactions Book
Author : K. Nagata,H. Handa
Publisher : Springer Science & Business Media
Release : 2013-12-11
ISBN : 4431669701
Language : En, Es, Fr & De

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Book Description :

The metabolism of all living organisms consists of elaborate and complex interactions among numbers of biomolecules, including protein-protein interactions. BIACORE is a new device that uses surface plasmon resonance for kinetic analysis of molecular interactions and automates whole analytical processes. The number of users of BIACORE is increasing worldwide, but until now nothing had been published in English to explain the principles of this new methodology and the kinds of applications it makes available. This updated and revised version of the Japanese original explains the underlying principles of BIACORE, providing concrete examples that utilize the methodology for analysis in areas ranging from basic to applied sciences. With its generous use of illustrations, this book is a valuable source of information for all users of BIACORE.

Handbook of Biophotonics

Handbook of Biophotonics Book
Author : Jürgen Popp,Valery V. Tuchin,Arthur Chiou,Stefan H. Heinemann
Publisher : John Wiley & Sons
Release : 2011-11-30
ISBN : 3527410481
Language : En, Es, Fr & De

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Book Description :

This debut edition of Visualizing Physical Geography encompasses the science of physical geography from local to global scales, using a uniquely visual approach to take students on a journey from the top layers of the Earth’s atmosphere to the rocks underlying the ocean basins to the forests of the farthest continents. As students explore the critical topics of physical geography, their study of the climate, surface features, and habitats around the world uses basic concepts of ecology, geology, chemistry, environmental science, biology, and physics and many other disciplines. Visualizing Physical Geography reinforces these interacting components and, with its premier art program, vividly illustrates the interconnectedness of physical processes that weave together to create our planet’s dynamic surface and atmosphere. Visualizing Geography relies heavily on other visuals with text to elucidate concepts for students and solidify their understanding of them. The goal is to help students understand the world around them and interpret what they see in a meaningful, accurate and exciting way. The content, design and layout of the text takes advantage of the full capacity in which students process information – visual as well as verbal. Visualizing Geography also helps students examine their own personal studying and learning styles with several new pedagogical aids –– encouraging students to apply what they are learning to their everyday lives. Visualizing Geography continues to offer ongoing study tips and psychological techniques for mastering the material. Most importantly students are provided with numerous opportunities to immediately access their understanding.

Metallic Nanocrystallites and their Interaction with Microbial Systems

Metallic Nanocrystallites and their Interaction with Microbial Systems Book
Author : Anil K. Suresh
Publisher : Springer Science & Business Media
Release : 2012-03-02
ISBN : 9400742312
Language : En, Es, Fr & De

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Book Description :

Although interactions between nanoparticles and microorganisms in the environment are unavoidable and commonplace, it is still not clear what potential effects they may have. Metallic Nanocrystallites and their Interface with Microbial Systems not only illustrates how microbes and these particular nanoparticles interact but also it describes the consequences of these interactions. This brief discusses the impact of gold, silver, zinc oxide, and cerium oxide nanoparticles on the growth and viability of both Gram-negative and Gram-positive bacterium. Moreover, it analyses the relationship between bacterial growth inhibition, reactive oxygen species generation, the regulation of transcriptional stress genomes, and the toxicity of these materials. Finally, it reviews the specific metallic nanomaterials and highlights their modes of synthesis, reactivity at surfaces, and the importance of assay procedures in determining their toxicity levels. Various microscopy techniques used to determine their mechanisms of action are also presented. Metallic Nanocrystallites and their Interface with Microbial Systems will be a valuable source to the scientific and industrial community as well as to students and researchers in microbiology, biotechnology, nanotechnology, toxicology, materials science, biomedical engineering, cell and molecular biology.

Single Molecule Enzymology Fluorescence Based and High Throughput Methods

Single Molecule Enzymology  Fluorescence Based and High Throughput Methods Book
Author : Anonim
Publisher : Academic Press
Release : 2016-10-28
ISBN : 0128095474
Language : En, Es, Fr & De

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Book Description :

Single-Molecule Enzymology, Part A, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, nanopores, and tethered particle motion. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in single-molecule enzymology Contains sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, nanopores, and tethered particle motion

Introduction to Computational Health Informatics

Introduction to Computational Health Informatics Book
Author : Arvind Kumar Bansal,Javed Iqbal Khan,S. Kaisar Alam
Publisher : CRC Press
Release : 2020-01-08
ISBN : 1000761592
Language : En, Es, Fr & De

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Book Description :

This class-tested textbook is designed for a semester-long graduate or senior undergraduate course on Computational Health Informatics. The focus of the book is on computational techniques that are widely used in health data analysis and health informatics and it integrates computer science and clinical perspectives. This book prepares computer science students for careers in computational health informatics and medical data analysis. Features Integrates computer science and clinical perspectives Describes various statistical and artificial intelligence techniques, including machine learning techniques such as clustering of temporal data, regression analysis, neural networks, HMM, decision trees, SVM, and data mining, all of which are techniques used widely used in health-data analysis Describes computational techniques such as multidimensional and multimedia data representation and retrieval, ontology, patient-data deidentification, temporal data analysis, heterogeneous databases, medical image analysis and transmission, biosignal analysis, pervasive healthcare, automated text-analysis, health-vocabulary knowledgebases and medical information-exchange Includes bioinformatics and pharmacokinetics techniques and their applications to vaccine and drug development

Analyzing Biomolecular Interactions by Mass Spectrometry

Analyzing Biomolecular Interactions by Mass Spectrometry Book
Author : Jeroen Kool,Wilfried M. A. Niessen
Publisher : John Wiley & Sons
Release : 2015-02-09
ISBN : 3527673423
Language : En, Es, Fr & De

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Book Description :

This monograph reviews all relevant technologies based on mass spectrometry that are used to study or screen biological interactions in general. Arranged in three parts, the text begins by reviewing techniques nowadays almost considered classical, such as affinity chromatography and ultrafiltration, as well as the latest techniques. The second part focusses on all MS-based methods for the study of interactions of proteins with all classes of biomolecules. Besides pull down-based approaches, this section also emphasizes the use of ion mobility MS, capture-compound approaches, chemical proteomics and interactomics. The third and final part discusses other important technologies frequently employed in interaction studies, such as biosensors and microarrays. For pharmaceutical, analytical, protein, environmental and biochemists, as well as those working in pharmaceutical and analytical laboratories.

Circular Dichroism and Linear Dichroism

Circular Dichroism and Linear Dichroism Book
Author : Alison Rodger,Bengt Nordén,University Lecturer Department of Chemistry Alison Rodger,Professor of Physical Chemistry Department of Physical Chemistry Bengt Nordén
Publisher : Oxford University Press, USA
Release : 1997
ISBN : 9780198558972
Language : En, Es, Fr & De

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Book Description :

Circular dichroism (CD) and linear dichroism (LD) are spectroscopic techniques that are becoming increasingly important probes of molecular structure, especially with proteins and DNAs. This text, the first in the new Oxford Chemistry Masters series, is designed to show how these two techniques can be used to provide the reader with both an intuitive and a quantitative understanding of how the techniques can be used.

Physical Methods for Inorganic Biochemistry

Physical Methods for Inorganic Biochemistry Book
Author : John R. Wright,Wayne A. Hendrickson,Shigemasa Osaki,Gordon T. James
Publisher : Springer Science & Business Media
Release : 2012-12-06
ISBN : 1468449974
Language : En, Es, Fr & De

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Book Description :

This volume is intended for students and professionals in diverse areas of the biological and biochemical sciences. It is oriented to those who are unfamiliar with the use of physical methods in studies of the biological elements. We hope the reader will find the material a helpful reference for other volumes of this series as well as the general literature, and some may see ways to adopt these techniques in their own pursuits. Every effort has been made to avoid an abstruse presentation. It should be clear that one individual cannot be expert in all the disciplines considered here (and the authors recognize that fact with sin cere humility). As may be expected of an introductory reference, most of our attention was focused on the commonly used methods. To balance this, we have included a few examples of approaches which are promising but relatively undeveloped at this time. Also, an emphasis has been placed on element selectivity. It is impossible to envision the course of future events, and a volume which deals with instrumentation is especially prone to become outdated. Nevertheless, any valid approach to a scientific question should be applicable indefinitely.

Book of abstracts PHOTONICA2017 The Sixth International School and Conference on Photonics COST actions MP1406 and MP1402 H2020 MSCA RISE 2015 CARDIALLY workshop

Book of abstracts PHOTONICA2017 The Sixth International School and Conference on Photonics   COST actions  MP1406 and MP1402  H2020 MSCA RISE 2015 CARDIALLY workshop Book
Author : Marina Lekić,Aleksandar Krmpot
Publisher : Institut za nuklearne nauke VINČA
Release : 2017-08-28
ISBN : 8682441462
Language : En, Es, Fr & De

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Book Description :

Download Book of abstracts PHOTONICA2017 The Sixth International School and Conference on Photonics COST actions MP1406 and MP1402 H2020 MSCA RISE 2015 CARDIALLY workshop book written by Marina Lekić,Aleksandar Krmpot, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Biomolecules in Organic Solvents

Biomolecules in Organic Solvents Book
Author : Armando Gomez-Puyou
Publisher : CRC Press
Release : 1992-01-27
ISBN : 9780849348235
Language : En, Es, Fr & De

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Book Description :

Biomolecules and Organic Solvents discusses the behavior of enzymes and multi-enzyme complexes in organic solvents, in addition to the problem of water-protein interactions and the characteristics of water at interphases. It attempts to bridge the gap between the all water and the organic solvents systems from the point of view of enzyme structure and function. The mechanism of action of enzymes dispersed in anhydrous organic solvents and the biotechnological perspectives of the field are evaluated, and new information regarding the function and characteristics of complex multi-enzymatic systems and whole cells in organic solvents are examined. New developments regarding the nature of the water pool and interphases of reverse micelles and their enzyme kinetics are also explored. The characteristics and properties of enzymes trapped in reverse micelles are discussed throughout the book. Biomolecules in Organic Solvents is essential reading for biochemists, molecular biologists, and others working in related fields.

Photophysics of Ionic Biochromophores

Photophysics of Ionic Biochromophores Book
Author : Steen Brøndsted Nielsen,Jean Ann Wyer
Publisher : Springer Science & Business Media
Release : 2013-10-16
ISBN : 3642401902
Language : En, Es, Fr & De

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Book Description :

This book provides a concise overview of the photophysics and spectroscopy of bio chromophore ions. The book "Photophysics of Ionic Biochromophores" summarizes important recent advances in the spectroscopy of isolated biomolecular ions in vacuo, which has within the last decade become a highly active research field. Advanced instrumental apparatus and the steady increase in more and more powerful computers have made this development possible, both for experimentalists and theoreticians. Applied techniques described here include absorption and fluorescence spectroscopy, which are excellent indicators of environmental effects and can thus shed light on the intrinsic electronic structures of ions without perturbations from e.g. water molecules, counter ions, nearby charges, and polar amino acid residues. When compared with spectra of the chromophores in their natural environment, such spectra allow to identify possible perturbations. At the same time gas-phase spectra provide important benchmarks for quantum chemistry calculations of electronically excited states. This volume focuses on biological systems from protein biochromophores, e.g. the protonated Schiff-base retinal responsible for vision, and individual aromatic amino acids to peptides and whole proteins, studied using visible, ultraviolet and vacuum ultraviolet light. Work on DNA nucleotides and strands that are amenable to mass spectrometric studies because of the negatively charged sugarphosphate backbone are also presented. DNA strands represent an example of the interplay between multiple chromophores, which is even harder to model correctly than just single chromophores due to spatially extended excited states and weak coupling terms. The experimental techniques used to measure spectra and commonly used theoretical methods are described with a discussion on limitations and advantages. The volume includes an updated status of the field and interesting future directions such as cold ion spectroscopy.

Biomolecular Spectroscopy Advances from Integrating Experiments and Theory

Biomolecular Spectroscopy  Advances from Integrating Experiments and Theory Book
Author : Anonim
Publisher : Elsevier
Release : 2013-09-04
ISBN : 0124165974
Language : En, Es, Fr & De

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Book Description :

Published continuously since 1944, Advances in Protein Chemistry and Structural Biology has been a continuous, essential resource for protein chemists. Covering reviews of methodology and research in all aspects of protein chemistry, including purification/expression, proteomics, modeling and structural determination and design, each volume brings forth new information about protocols and analysis of proteins while presenting the most recent findings from leading experts in a broad range of protein-related topics. Covers reviews of methodology and research in all aspects of protein chemistry Brings forth new information about protocols and analysis of proteins while presenting the most recent findings from leading experts in a broad range of protein-related topics

Aspects of Physical Biology

Aspects of Physical Biology Book
Author : Giancarlo Franzese,Miguel Rubi
Publisher : Springer Science & Business Media
Release : 2008-07-10
ISBN : 354078764X
Language : En, Es, Fr & De

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Book Description :

The application to Biology of the methodologies developed in Physics is attracting an increasing interest from the scientific community. It has led to the emergence of a new interdisciplinary field, called Physical Biology, with the aim of reaching a better understanding of the biological mechanisms at molecular and cellular levels. Statistical Mechanics in particular plays an important role in the development of this new field. For this reason, the XXth session of the famous Sitges Conference on Statistical Physics was dedicated to "Physical Biology: from Molecular Interactions to Cellular Behavior". As is by now tradition, a number of lectures were subsequently selected, expanded and updated for publication as lecture notes, so as to provide both a state-of-the-art introduction and overview to a number of subjects of broader interest and to favor the interchange and cross-fertilization of ideas between biologists and physicists. The present volume focuses on three main subtopics (biological water, protein solutions as well as transport and replication), presenting for each of them the on-going debates on recent results. The role of water in biological processes, the mechanisms of protein folding, the phases and cooperative effects in biological solutions, the thermodynamic description of replication, transport and neural activity, all are subjects that are revised in this volume, based on new experiments and new theoretical interpretations.

Single Molecule Tools Part B Super Resolution Particle Tracking Multiparameter and Force Based Methods

Single Molecule Tools  Part B  Super Resolution  Particle Tracking  Multiparameter  and Force Based Methods Book
Author : Anonim
Publisher : Academic Press
Release : 2010-07-09
ISBN : 9780123814838
Language : En, Es, Fr & De

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Book Description :

Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. Single Molecule Tools, Part B: Super-Resolution, Particle Tracking, Multiparameter, and Force Based Methods captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools. Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools Methods included are useful to both established researchers and newcomers to the field Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines

Annual Reports on NMR Spectroscopy

Annual Reports on NMR Spectroscopy Book
Author : Graham A. Webb
Publisher : Academic Press
Release : 2010-04-12
ISBN : 0123813549
Language : En, Es, Fr & De

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Book Description :

In recent years, no other technique has gained such significance as NMR spectroscopy. It is used in all branches of science in which precise structural determination is required and in which the nature of interactions and reactions in solution is being studied. Annual Reports on NMR Spectroscopy has established itself as a premier means for the specialist and non-specialist alike to become familiar with new techniques and applications of NMR spectroscopy. * Provides updates on the latest developments in NMR spectroscopy * Includes comprehensive review articles * Highlights the increasing importance of NMR spectroscopy as a technique for structural determination

Concepts of Biology

Concepts of Biology Book
Author : Samantha Fowler,Rebecca Roush,James Wise
Publisher : Unknown
Release : 2018-01-07
ISBN : 9789888407453
Language : En, Es, Fr & De

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Book Description :

Concepts of Biology is designed for the single-semester introduction to biology course for non-science majors, which for many students is their only college-level science course. As such, this course represents an important opportunity for students to develop the necessary knowledge, tools, and skills to make informed decisions as they continue with their lives. Rather than being mired down with facts and vocabulary, the typical non-science major student needs information presented in a way that is easy to read and understand. Even more importantly, the content should be meaningful. Students do much better when they understand why biology is relevant to their everyday lives. For these reasons, Concepts of Biology is grounded on an evolutionary basis and includes exciting features that highlight careers in the biological sciences and everyday applications of the concepts at hand.We also strive to show the interconnectedness of topics within this extremely broad discipline. In order to meet the needs of today's instructors and students, we maintain the overall organization and coverage found in most syllabi for this course. A strength of Concepts of Biology is that instructors can customize the book, adapting it to the approach that works best in their classroom. Concepts of Biology also includes an innovative art program that incorporates critical thinking and clicker questions to help students understand--and apply--key concepts.

Imaging from Cells to Animals In Vivo

Imaging from Cells to Animals In Vivo Book
Author : Margarida Barroso,Xavier Intes
Publisher : CRC Press
Release : 2020-12-03
ISBN : 1351704494
Language : En, Es, Fr & De

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Book Description :

This book offers an overview of imaging techniques used to investigate cells and tissue in their native environment. It covers the range of imaging approaches used, as well as the application of those techniques to the study of biological processes in cells and whole tissues within living organisms.