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Introduction To Protein Mass Spectrometry

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Introduction to Protein Mass Spectrometry

Introduction to Protein Mass Spectrometry Book
Author : Pradip Kumar Ghosh
Publisher : Academic Press
Release : 2015-12-10
ISBN : 0128021128
Language : En, Es, Fr & De

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Book Description :

Introduction to Protein Mass Spectrometry provides a comprehensive overview of this increasingly important, yet complex, analytical technique. Unlike many other methods which automatically yield an absolutely unique protein name as output, protein mass spectrometry generally requires a deduction of protein identity from determination of peptide fragmentation products. This book enables readers to both understand, and appreciate, how determinations about protein identity from mass spectrometric data are made. Coverage begins with the technical basics, including preparations, instruments, and spectrometric analysis of peptides and proteins, before exploring applied use in biological applications, bioinformatics, database, and software resources. Citing the most recent and relevant work in the field of biological mass spectrometry, the book is written for researchers and scientists new to the field, but is also an ideal resource for those hoping to hone their analytical abilities. Offers introductory information for scientists and researchers new to the field, as well as advanced insight into the critical assessment of computer-analyzed mass spectrometric results and their current limitations Provides examples of commonly-used MS instruments from Bruker, Applied Biosystems, JEOL, Thermo Scientific/Thermo Fisher Scientific, IU, and Waters Includes biological applications and exploration of analytical tools and databases for bioinformatics

Introduction to Protein Mass Spectrometry

Introduction to Protein Mass Spectrometry Book
Author : Pradip Kumar Ghosh
Publisher : Academic Press
Release : 2015-12-15
ISBN : 9780128051238
Language : En, Es, Fr & De

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Book Description :

Introduction to Protein Mass Spectrometry provides a comprehensive overview of this increasingly important, yet complex, analytical technique. Unlike many other methods which automatically yield an absolutely unique protein name as output, protein mass spectrometry generally requires a deduction of protein identity from determination of peptide fragmentation products. This book enables readers to both understand, and appreciate, how determinations about protein identity from mass spectrometric data are made. Coverage begins with the technical basics, including preparations, instruments, and spectrometric analysis of peptides and proteins, before exploring applied use in biological applications, bioinformatics, database, and software resources. Citing the most recent and relevant work in the field of biological mass spectrometry, the book is written for researchers and scientists new to the field, but is also an ideal resource for those hoping to hone their analytical abilities. Offers introductory information for scientists and researchers new to the field, as well as advanced insight into the critical assessment of computer-analyzed mass spectrometric results and their current limitations Provides examples of commonly-used MS instruments from Bruker, Applied Biosystems, JEOL, Thermo Scientific/Thermo Fisher Scientific, IU, and Waters Includes biological applications and exploration of analytical tools and databases for bioinformatics

Introduction to Mass Spectrometry

Introduction to Mass Spectrometry Book
Author : J. Throck Watson,O. David Sparkman
Publisher : John Wiley & Sons
Release : 2013-07-09
ISBN : 1118681584
Language : En, Es, Fr & De

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Book Description :

Completely revised and updated, this text provides an easy-to-read guide to the concept of mass spectrometry and demonstrates its potential and limitations. Written by internationally recognised experts and utilising "real life" examples of analyses and applications, the book presents real cases of qualitative and quantitative applications of mass spectrometry. Unlike other mass spectrometry texts, this comprehensive reference provides systematic descriptions of the various types of mass analysers and ionisation, along with corresponding strategies for interpretation of data. The book concludes with a comprehensive 3000 references. This multi-disciplined text covers the fundamentals as well as recent advance in this topic, providing need-to-know information for researchers in many disciplines including pharmaceutical, environmental and biomedical analysis who are utilizing mass spectrometry

Introduction to Proteomics

Introduction to Proteomics Book
Author : Daniel Liebler
Publisher : Springer Science & Business Media
Release : 2001-12-04
ISBN : 9780896039919
Language : En, Es, Fr & De

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Book Description :

Daniel C. Liebler masterfully introduces the science of proteomics by spelling out the basics of how one analyzes proteins and proteomes, and just how these approaches are then employed to investigate their roles in living systems. He explains the key concepts of proteomics, how the analytical instrumentation works, what data mining and other software tools do, and how these tools can be integrated to study proteomes. Also discussed are how protein and peptide separation techniques are applied in proteomics, how mass spectrometry is used to identify proteins, and how data analysis software enables protein identification and the mapping of modifications. In addition, there are proteomic approaches for analyzing differential protein expression, characterizing proteomic diversity, and dissecting protein-protein interactions and networks.

Introducing Proteomics

Introducing Proteomics Book
Author : Josip Lovric
Publisher : John Wiley & Sons
Release : 2011-06-17
ISBN : 1119957192
Language : En, Es, Fr & De

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Book Description :

Introducing Proteomics gives a concise and coherent overview of every aspect of current proteomics technology, which is a rapidly developing field that is having a major impact within the life and medical sciences. This student-friendly book, based on a successful course developed by the author, provides its readers with sufficient theoretical background to be able to plan, prepare, and analyze a proteomics study. The text covers the following: Separation Technologies Analysis of Peptides/Proteins by Mass Spectrometry Strategies in Proteomics This contemporary text also includes numerous examples and explanations for why particular strategies are better than others for certain applications. In addition, Introducing Proteomics includes extensive references and a list of relevant proteomics information sources; essential for any student. This no-nonsense approach to the subject tells students exactly what they need to know, leaving out unnecessary information. The student companion site enhances learning and provides answers to the end of chapter problems. "I think this book will be a popular and valuable resource for students and newcomers to the field who would like to have an overview and initial understanding of what proteomics is about. The contents are well organized and address the major issues." —Professor Walter Kolch, Director, Systems Biology Ireland & Conway Institute, University College Dublin Companion Website www.wiley.com/go/lovric

Introduction to Proteomics

Introduction to Proteomics Book
Author : Nawin C. Mishra
Publisher : John Wiley & Sons
Release : 2011-09-19
ISBN : 047092084X
Language : En, Es, Fr & De

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Book Description :

Proteomics provides an introductory insight on proteomics, discussing the basic principles of the field, how to apply specific technologies and instrumentation, and example applications in human health and diseases. With helpful study questions, this textbook presents an easy to grasp and solid overview and understanding of the principles, guidelines, and especially the complex instrumentation operations in proteomics for new students and research scientists. Written by a leader in proteomics studies, Proteomics offers an expert perspective on the field and the future of proteomics.

Mass Spectrometry Developmental Approaches to Answer Biological Questions

Mass Spectrometry  Developmental Approaches to Answer Biological Questions Book
Author : Gwenael Pottiez
Publisher : Springer
Release : 2015-02-05
ISBN : 3319130870
Language : En, Es, Fr & De

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Book Description :

The understanding of the events taking place in a cell, a biological fluid or in any biological system is the main goal of biology research. Many fields of research use different technology to assess those events. Mass spectrometry is one of those techniques and this undergoes constant evolution and adaptation to always enhance the accuracy of the information provided. Proteomics provides a large panel of data on protein identity and protein expression that were made possible by mass spectrometry. For several years now mass spectrometry has become central to performing proteomic research, however this powerful tool is under constant evolution to be more sensitive and more resolute. More importantly mass spectrometry became a field of research focusing on new applications. Indeed, the complexity in biological systems relies on the changes of expression of transcription of proteins but also on the post-translational modification of proteins, the structure of proteins and the interaction between proteins, amongst others. As of now, several investigations tried to improve the quantification of proteins by mass spectrometry, the determination of post-translational modifications, the protein-protein and protein-nucleic acids interaction or the proteins structures. This book is structured as follows: after a brief introduction of the usual and most popular applications for mass spectrometry in proteomics, the most recent research and developments in mass spectrometry-based methodologies will be explored.

Introduction to Protein Science

Introduction to Protein Science Book
Author : Arthur M. Lesk,Lesk,Professor of Biochemistry and Molecular Biology Arthur M Lesk
Publisher : Oxford University Press, USA
Release : 2004
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Useful for students on biosciences degrees, this book provides an introduction to the study of proteins. It contains the aspects related to genomics and proteomics that have paved the way for an explosion of interest in protein structure and function.

Characterization of Protein Therapeutics using Mass Spectrometry

Characterization of Protein Therapeutics using Mass Spectrometry Book
Author : Guodong Chen
Publisher : Springer Science & Business Media
Release : 2014-07-08
ISBN : 1441978623
Language : En, Es, Fr & De

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Book Description :

This book highlights current approaches and future trends in the use of mass spectrometry to characterize protein therapies. As one of the most frequently utilized analytical techniques in pharmaceutical research and development, mass spectrometry has been widely used in the characterization of protein therapeutics due to its analytical sensitivity, selectivity, and specificity. This book begins with an overview of mass spectrometry techniques as related to the analysis of protein therapeutics, structural identification strategies, quantitative approaches, followed by studies involving characterization of process related protein drug impurities/degradants, metabolites, higher order structures of protein therapeutics. Both general practitioners in pharmaceutical research and specialists in analytical sciences will benefit from this book that details step-by-step approaches and new strategies to solve challenging problems related to protein therapeutics research and development.

Fundamentals of Contemporary Mass Spectrometry

Fundamentals of Contemporary Mass Spectrometry Book
Author : Chhabil Dass
Publisher : John Wiley & Sons
Release : 2007-05-11
ISBN : 0470118482
Language : En, Es, Fr & De

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Book Description :

Modern mass spectrometry - the instrumentation and applications in diverse fields Mass spectrometry has played a pivotal role in a variety of scientific disciplines. Today it is an integral part of proteomics and drug discovery process. Fundamentals of Contemporary Mass Spectrometry gives readers a concise and authoritative overview of modern mass spectrometry instrumentation, techniques, and applications, including the latest developments. After an introduction to the history of mass spectrometry and the basic underlying concepts, it covers: Instrumentation, including modes of ionization, condensed phase ionization techniques, mass analysis and ion detection, tandem mass spectrometry, and hyphenated separation techniques Organic and inorganic mass spectrometry Biological mass spectrometry, including the analysis of proteins and peptides, oligosaccharides, lipids, oligonucleotides, and other biological materials Applications to quantitative analysis Based on proven teaching principles, each chapter is complete with a concise overview, highlighted key points, practice exercises, and references to additional resources. Hints and solutions to the exercises are provided in an appendix. To facilitate learning and improve problem-solving skills, several worked-out examples are included. This is a great textbook for graduate students in chemistry, and a robust, practical resource for researchers and scientists, professors, laboratory managers, technicians, and others. It gives scientists in diverse disciplines a practical foundation in modern mass spectrometry.

Computational Methods for Mass Spectrometry Proteomics

Computational Methods for Mass Spectrometry Proteomics Book
Author : Ingvar Eidhammer,Kristian Flikka,Lennart Martens,Svein-Ole Mikalsen
Publisher : John Wiley & Sons
Release : 2008-02-28
ISBN : 9780470724293
Language : En, Es, Fr & De

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Book Description :

Proteomics is the study of the subsets of proteins present in different parts of an organism and how they change with time and varying conditions. Mass spectrometry is the leading technology used in proteomics, and the field relies heavily on bioinformatics to process and analyze the acquired data. Since recent years have seen tremendous developments in instrumentation and proteomics-related bioinformatics, there is clearly a need for a solid introduction to the crossroads where proteomics and bioinformatics meet. Computational Methods for Mass Spectrometry Proteomics describes the different instruments and methodologies used in proteomics in a unified manner. The authors put an emphasis on the computational methods for the different phases of a proteomics analysis, but the underlying principles in protein chemistry and instrument technology are also described. The book is illustrated by a number of figures and examples, and contains exercises for the reader. Written in an accessible yet rigorous style, it is a valuable reference for both informaticians and biologists. Computational Methods for Mass Spectrometry Proteomics is suited for advanced undergraduate and graduate students of bioinformatics and molecular biology with an interest in proteomics. It also provides a good introduction and reference source for researchers new to proteomics, and for people who come into more peripheral contact with the field.

Proteomics

Proteomics Book
Author : Timothy Palzkill
Publisher : Springer Science & Business Media
Release : 2007-05-08
ISBN : 0306468956
Language : En, Es, Fr & De

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Book Description :

Proteomics is an introduction to the exciting new field of proteomics, an interdisciplinary science that includes biology, bioinformatics, and protein chemistry. The purpose of this book is to provide the active researcher with an overview of the types of questions being addressed in proteomics studies and the technologies used to address those questions. Key subjects covered in this book include: an assessment of the limitations of this approach and outlines new developments in mass spectrometry that will advance future research high-throughput recombinant DNA cloning methods used to systematically clone all of the open reading frames of an organism into plasmid vectors for large scale protein expression and functional studies such as protein-protein interactions with the two-hybrid system protein structure an overview of large-scale experimental attempts to determine the three-dimensional structures of representative sets of proteins computational approaches to determining the three-dimensional structure of proteins. Proteomics provides a starting point for researchers who would like a theoretical understanding of the new technologies in the field, and obtain a solid grasp of the fundamentals before integrating new tools into their experiments. Written with attention to detail, but without being overwhelmingly technical, Proteomics is a user-friendly guide needed by most biologists today.

Mass Spectrometry

Mass Spectrometry Book
Author : Jürgen H Gross
Publisher : Springer
Release : 2017-06-13
ISBN : 3319543989
Language : En, Es, Fr & De

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Book Description :

This book offers a balanced mixture of practice-oriented information and theoretical background as well as numerous references, clear illustrations, and useful data tables. Problems and solutions are accessible via a special website. This new edition has been completely revised and extended; it now includes three new chapters on tandem mass spectrometry, interfaces for sampling at atmospheric pressure, and inorganic mass spectrometry.

Introduction to Protein Sequence Analysis

Introduction to Protein Sequence Analysis Book
Author : L. R. Croft
Publisher : John Wiley & Sons
Release : 1980
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Introduction to Protein Sequence Analysis book written by L. R. Croft, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Protein Identification and Characterization by Mass Spectrometry

Protein Identification and Characterization by Mass Spectrometry Book
Author : Joy M. Ginter
Publisher : Unknown
Release : 2008
ISBN : 9780549754404
Language : En, Es, Fr & De

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Book Description :

This work examines the use of mass spectrometry for protein identification and characterization. Although techniques from the three current proteomic approaches, bottom-up, shotgun, and top-down, were studied, the main research goal was to develop improved top-down methodology in light of the shortcomings found within other techniques. Mass spectrometry instrumentation not previously suited for top-down analysis of relatively large, intact proteins was used in a modified format. A pseudo MS3 approach was developed using a commercially available quadrupole-time-of-flight mass spectrometer (Q-TOF) through the use of in-source collision induced dissociation (CID) coupled to a more traditional CID experiment utilizing the collision cell of the mass spectrometer. With this approach, sequence tags originating from the termini of the proteins were sequenced that led to unique protein identifications. Experiments showed that for the method to be successful proteins first needed to be in a reduced state as disulfide bonds within the protein's primary structure inhibited fragmentation. This technique was demonstrated with simple protein standard mixtures and then applied to a more complex protein mixture, the proteome of E. Coli . Complex protein mixtures require significant separation prior to introduction into the mass spectrometer, and methods for introducing intact proteins are not as prevalent. Solution isoelectric focusing (sIEF) of whole proteins was studied using a modified commercial isoelectric focusing (IEF) device, and the resulting fractions electrosprayed directly into the mass spectrometer after separation by liquid chromatography (LC). The focus of this work was to develop a method that would be analogous to the traditional 2D gel, but amenable to use with the developed top-down MS approach. The proteome of E. Coli was separated using the sIEF-LC approach, and the resulting fractions were subjected to two different MS based approaches. In the first approach, the intact masses of soluble proteins within each sIEF fraction were determined through the deconvolution of each protein's MS spectrum. The pseudo MS3 approach was then applied to generate sequence tags for the proteins to aid in the identification of the proteins. In this first approach a researcher has a more accurate mass, a pI range, and a sequence tag all available for use when determining a protein's identification. In the second approach used the sIEF fractions were globally digested with the enzyme trypsin, and the resulting tryptic digest solutions were analyzed using a shotgun proteomic method. In this approach, proteins were identified through the sequencing of their tryptic fragments and the pI range from the sIEF. This approach was used to determine if any mass limitations existed within the LC separation of the intact proteins. In the first approach, the highest molecular mass observed was approximately 40kDa whereas in the second approach tryptic fragments were found to match to proteins up to 52kDa. Top-down proteomic approaches have gained a lot of attention as researchers shift their focus from simply identifying proteins to characterizing them in light of a specific question. The pseudo-MS3 approach described herein is advantageous since it allows for characterizing relatively large intact proteins with instrumentation that is already common in most proteomic laboratories. Previous top-down approaches have mainly relied on the use of more advanced MS instrumentation such as a Fourier Transform mass spectrometer (FT-MS). Additionally, the sIEF-LC approach described allows for fractionated proteins that can be used for any of a number of proteomic approaches including a top-down approach where solution-based separations are necessary.

Proteomics of Biological Systems

Proteomics of Biological Systems Book
Author : Bryan M. Ham
Publisher : John Wiley & Sons
Release : 2011-11-15
ISBN : 1118028961
Language : En, Es, Fr & De

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Book Description :

Phosphorylation is the addition of a phosphate (PO 4 ) group to a protein or other organic molecule. Phosphorylation activates or deactivates many protein enzymes, causing or preventing the mechanisms of diseases such as cancer and diabetes. This book shows how to use mass spectrometry to determine whether or not a protein has been correctly modified by the addition of a phosphate group. It also provides a combination of detailed, step–by–step methodology for phosphoproteomic sample preparation, mass spectral instrumental analysis, and data interpretation approaches. Furthermore, it includes the use of bioinformatic Internet tools such as the Blast2GO gene ontology (GO) tool, used to help understand and interpret complex data collected in these studies.

Protein and Peptide Analysis by Mass Spectrometry

Protein and Peptide Analysis by Mass Spectrometry Book
Author : John R. Chapman
Publisher : Humana Press
Release : 2013-08-11
ISBN : 9781489940735
Language : En, Es, Fr & De

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Book Description :

The purpose of the preface is to explain the book's objectives and how to use it; give warnings, disclaimers, and the like.* The main objective of Protein and Peptide Analysis by Mass Spec trometry is quite straightforward—to present authoritative, up-to-date, and practical accounts of the use of mass spectrometry in the analysis of pep tides and proteins. How to use it? Every reader will have their own particular interests and will surely be drawn toward the chapters that cover these interests. Within the remaining chapters, however, techniques are described with analytical possibilities that such a reader can then only guess at. So, read the book fully. Again, as is customary in the Methods in Molecular Biology series, the chapter format (Introduction, Materials, Methods, and Notes) allows the authors to introduce the techniques, to explain their relevance and applicability, and, above all, to provide detail—detail that represents each author's accumulated experience and enables the reader to use and benefit from these methods. So, read the book fully, and read it diligently. Warnings and disclaimers: Mass spectrometry today offers the pro tein chemist ready access to a wealth of information that is otherwise avail able only with great difficulty, or perhaps not at all. With this goal in sight, any warnings and disclaimers will almost surely be ignored. So, a warning anyway; the use of mass spectrometry might be habit forming.

Genetic Engineering Biotechnology News

Genetic Engineering   Biotechnology News Book
Author : Anonim
Publisher : Unknown
Release : 2007
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Genetic Engineering Biotechnology News book written by , available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Introduction to Mass Spectrometry

Introduction to Mass Spectrometry Book
Author : J. Throck Watson
Publisher : Lippincott Williams & Wilkins
Release : 1997
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Completely revised and updated, this third edition text aims to provide an easy-to-read guide to the concept of mass spectrometry, demonstrating its potential and limitations. Utilizing real life examples of analyses and applications, the text presents 18 realistic cases of qualitative and quantitative applications of mass spectrometry. It provides systematic references of various types of mass analyzers and ionization, along with corresponding strategies for interpretation of data. Detailed coverage of inlet systems, vacuum systems, detectors, data systems, and specialized techniques such as MS/MS and selected ion monitoring for quantitative analyses is included.

Clinical Proteomics

Clinical Proteomics Book
Author : Antonia Vlahou
Publisher : Springer Science & Business Media
Release : 2008-01-18
ISBN : 158829837X
Language : En, Es, Fr & De

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Book Description :

In this book, a select group of researchers has contributed their state-of-the-art methodologies on protein profiling and identification of disease biomarkers in tissues, microdissected cells and body fluids. The book integrates biochemistry, pathology, analytical technology, bioinformatics, and proteome informatics. Experimental approaches are thoroughly detailed and explained through a step-by-step instructional format that ensures successful results.