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In Situ Molecular Pathology And Co Expression Analyses

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In Situ Molecular Pathology and Co expression Analyses

In Situ Molecular Pathology and Co expression Analyses Book
Author : Gerard J. Nuovo
Publisher : Academic Press
Release : 2020-09-03
ISBN : 0128206543
Language : En, Es, Fr & De

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Book Description :

Major advancements in the field of in situ molecular pathology have occurred since publication of the first edition. In Situ Molecular Pathology and Co-expression Analyses, Second Edition, continues to teach both the molecular basis for the improvements and the actual protocols. This is the unique feature that separates it from the pack of other "cook-book" type approaches. The fields of in situ hybridization and immunohistochemistry have expanded rapidly where computer-based analyses systems have greatly expanded the power of these methods. Further, knowledge of the marked improvements in the reagents themselves since the first edition can make the difference of excellent versus misleading data. The automated platforms require that researchers and diagnostic biomedical investigators have a good understanding of the basics of in situ based tests, protocols, and biochemistry for troubleshooting in order to maximize the use of these platforms. This second edition focuses attention on straightforward protocols used to simultaneously detect two or more proteins/nucleic acids within intact tissue by doing co-expression analyses. Practicing molecular pathologists, diagnostic pathologists, laboratory directors, and toxicologists, as well as clinicians and researchers in training, will benefit from this clear presentation of protocols and theoretical framework. Data derived from in situ hybridization and immunohistochemistry. Explains the theory and foundation of immunohistochemistry and in situ hybridization and presents easy-to-follow experimental protocols with tricks of the trade Includes two new chapters: Recent improvements in immunohistochemistry and in situ hybridization, Quality control for immunohistochemistry and in situ hybridization: How to know if the color change is signal or background The second edition also includes a detailed test to help one learn the basics of histologic interpretation of tissues and a separate detailed test in how to differentiate signal from background Includes chapter-ending summaries of Key Points to Remember, bringing beginners up to speed with any seasoned veteran in the field Thoughtfully tackles the molecular basis if IHC and ISH, along with application of that knowledge to improving the techniques is significant

In Situ Molecular Pathology and Co Expression Analyses

In Situ Molecular Pathology and Co Expression Analyses Book
Author : Gerard J. Nuovo
Publisher : Academic Press
Release : 2013-02-07
ISBN : 0124160018
Language : En, Es, Fr & De

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Book Description :

In Situ Molecular Pathology and Co-Expression Analyses explains, in easy-to-understand language, simplified ways of understanding and performing in situ hybridization and immunohistochemistry tests. The book also focuses on straightforward protocols used to simultaneously detect two or more proteins/nucleic acids within intact tissue by doing co-expression analyses. The fields of in situ hybridization and immunohistochemistry have expanded rapidly due to the use of computer-based analysis. To get the most out of these automated platforms, researchers and diagnostic biomedical investigators must have a solid understanding of the basics of in situ-based tests, protocols, and regimens for troubleshooting. Practicing molecular pathologists, clinical chemists, and toxicologists, as well as clinicians and researchers in training, will benefit from this book's clear presentation of protocols and theoretical framework. Includes over 200 easy-to-follow experimental protocols Features chapter-ending summaries of "Key Points to Remember" to bring beginners up to speed with any seasoned veteran in the field Offers two chapters written by industry leaders in the fields of in situ hybridization, immunohistochemistry, and computer software for co-expression analyses

Molecular Biology in Cellular Pathology

Molecular Biology in Cellular Pathology Book
Author : John Crocker,Paul G. Murray
Publisher : John Wiley & Sons
Release : 2003-09-12
ISBN : 0470864982
Language : En, Es, Fr & De

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Book Description :

The latest edition of this highly successful text, covers the major advances in the methods used in cellular and molecular pathology. In recent years, knowledge of the molecular organization of the cell has led to the development of powerful new techniques that bring greater accuracy and objectives to the diagnosis, prognosis and management of many diseases and to the study of pathological states. This book describes the latest molecular techniques available for the analysis of diseases. In particular it includes new techniques using fluorescent dyes, DNA microarrays, protein chemistry, and mass spectrometry. It also incorporates information from the Human Genome Project, and the new disciplines of genomics and proteomics, where relevant to pathology. Color plates are a new feature of this edition, illustrating the advances in fluorescence labeling of cells.

Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas

Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas Book
Author : M. A. Hayat
Publisher : Elsevier
Release : 2005-01-31
ISBN : 0080495206
Language : En, Es, Fr & De

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Book Description :

Immunohistochemistry is the use of specific antibodies to stain particular molecular species in situ. This technique has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the endocrine system, and has the potential to improve diagnosis, prognosis and therapeutic options of cancer. Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step-by-step instructions on the methods of additional molecular technologies such as DNA microarrays, and microdissection, along with the benefits and limitations of each method. The topics of region-specific gene expression, its role in cancer development and the techniques that assist in the understanding of the molecular basis of disease are relevant and necessary in science today. This book is the second volume of three planned, individually-sold volumes on this topic. Like Volume 1, this book fully explains the principles and applications of modern techniques used in the field of molecular genetics. It will be of particular interest to pathologists and molecular pathologists conducting both academic and/or clinical research. The only book available that translates molecular genetics into cancer diagnosis The results of each Immunohistochemical and in situ hybridization method are presented in the form of color illustrations Methods discussed were either developed or refined by expert contributors in their own laboratories

Gene Probes

Gene Probes Book
Author : P. Michael Conn
Publisher : Academic Press
Release : 2014-04-24
ISBN : 1483267857
Language : En, Es, Fr & De

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Book Description :

Methods in Neurosciences, Volume 1: Gene Probes is a compendium of papers that deals with the developments in molecular biology, cell biology, and electrophysiology. Section I deals with gene expression using the Xenopus Oocyte system for expression and cloning of neuroreceptors and channels. One paper presents a method in studying the molecules in the brain related to neurotransmitter receptors and to the voltage channels in the brain by ""transplanting"" functional neurotransmitter receptors into the membrane of frog oocytes. Section II describes in situ and solution hybridization and the continuation of in situ hybridization with immunohistochemistry. One paper discusses the benefits of using alkaline phosphatase-Dig-dUTP-labeled oligonucleotide probes in high-resolution in situ in terms of ease of performance, safety, and fast detection rendering. Section III addresses the screening, sequencing, and cloning, the process of which includes the rapid identification of DNA clones. One paper outlines the methods and materials that are used in such identification. Section IV explains lineage analysis and Section V discusses molecular pathology, including the molecular pathology of Alzheimer's disease. This collection can prove useful for geneticists, molecular scientists, and academicians involved in neuroscience and pharmacological sciences, as well as researchers in geriatrics.

Archives of Pathology Laboratory Medicine

Archives of Pathology   Laboratory Medicine Book
Author : Anonim
Publisher : Unknown
Release : 2002
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Archives of Pathology Laboratory Medicine book written by , available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Modern Surgical Pathology

Modern Surgical Pathology Book
Author : Noel Weidner
Publisher : W B Saunders Company
Release : 2003
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

The electronic image collection consists of JPG and PDF files containing graphics from Modern surgical pathology.

Precision Molecular Pathology of Breast Cancer

Precision Molecular Pathology of Breast Cancer Book
Author : Ashraf Khan,Ian O. Ellis,Andrew M. Hanby,Ediz F. Cosar,Emad A. Rakha,Dina Kandil
Publisher : Springer
Release : 2015-08-12
ISBN : 1493928864
Language : En, Es, Fr & De

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Book Description :

This volume provides a comprehensive review of the molecular mechanisms involved in precancerous lesions and benign and malignant breast tumors. Given the complex molecular pathways in breast cancer biology, the book simplifies these complex mechanisms and highlights the practical issues important for daily practice. Sections are structured to review breast carcinogenesis and the role of familial predisposition and stem cells in initiation and progression of breast cancer. In-depth chapters present morphologic and molecular correlations in precancerous and malignant breast lesions, while outlining highly practical issues that are in practice today in breast pathology, such as evaluation of estrogen, progesterone receptors, and HER-2. Written by experts in the field, Precision Molecular Pathology of Breast Cancer is a valuable resource that covers the current practice of breast pathology and looks into the future with an emphasis on the molecular basis of breast cancer.

Orbital Tumors

Orbital Tumors Book
Author : John Warren Henderson
Publisher : Raven Press (ID)
Release : 1994
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

An introductory text for orbitologists, completely rewritten to reflect major developments pertaining to orbital tumors in the 13 years since publication of the second edition. Foremost in this regard is the routine use of soft tissue imaging methods for the preoperative clinical assessment of patients with orbital tumors, and the application of immunohistochemistry, monoclonal antibodies, and molecular genetic analysis to the classification and histopathological identification of many of the orbital lesions. Likewise, adjuvant chemotherapy has enlarged the management options of selected cases, and orbitectomy, rather than orbitotomy, has evolved for some of the malignant neoplasias. Annotation copyright by Book News, Inc., Portland, OR

Pancreatic Cancer

Pancreatic Cancer Book
Author : Fazlul H. Sarkar,Michael C. Dugan
Publisher : Eaton Publishing Company
Release : 1998
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Pancreatic Cancer book written by Fazlul H. Sarkar,Michael C. Dugan, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Molecular Pathology of Gastroenterological Cancer

Molecular Pathology of Gastroenterological Cancer Book
Author : Eiichi Tahara
Publisher : Springer
Release : 1997-10
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Twenty years have passed since I became a professor in the First Department of Pathology, Hiroshima University School of Medicine. It is my great pleas ure that Molecular Pathology o[ Gastroenterological Cancer-Application to Clinical Practice has been published by Springer-Verlag Tokyo to commemo rate the 20th anniversary of my professorship. Seeing the academic achievements of our department during these 20 years, I am confident that we could establish a department of oncology to research the pathogenesis of human cancer through systemic application of a variety of molecular techniques. We have demonstrated that the develop me nt and progression of esophageal, gastric, and colon cancer require mul tiple alterations affecting DNA mismatch repair genes, oncogenes, and tumorsuppressor genes, and that common and uncommon genetic changes exist for esophageal, gastric, and colorectal carcinomas. In addition to these genetic changes, the majority of gastrointestinal cancers express telomerase activity, with overexpression of telomerase RNA, indicating a powerful addi tional tool for early detection of gastrointestinal cancer. By transferring these basic observations to the clinic, we now are able to make accurate cancer diagnoses, thus determing the grade of malignancy and patient prognosis. We also can identify patients at high risk for developing cancer and create new therapeutic approaches. In fact, we have routinely implemented a new molecular diagnosis strategy at the Hiroshima City Medi cal Association Clinical Laboratory since August 1993.

Molecular Analysis of Cancer

Molecular Analysis of Cancer Book
Author : Jacqueline Boultwood,Carrie Fidler
Publisher : Springer Science & Business Media
Release : 2001
ISBN : 1592591353
Language : En, Es, Fr & De

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Book Description :

Molecular biotechnology has in recent years produced significant advances in finding tumor markers useful in diagnosis and in the assessment of disease progression and prognosis. In Molecular Analysis of Cancer, leading researchers in the field describe in step-by-step detail their best state-of-the-art molecular techniques for elucidating the biochemical processes associated with human cancer. Among the techniques presented for identifying chromosomal rearrangements are fluorescent in situ hybridization (FISH), spectral karyotyping (SKY), comparative genomic hybridization (CGH), microsatellite analysis, PCR and RT-PCR, and DNA fingerprinting. Additional methods include SSCP, DGGE, the nonisotopic RNase cleavage assay, and the protein truncation assay and DNA sequencing for identifying mutations. Techniques for the analysis of gene expression include microarray technology and differential display. All these techniques are of great value for identifying genes that are central to cancer development and progression. Comprehensive and state-of-the-art, Molecular Analysis of Cancer not only offers today's researcher a deeper understanding of cancer's molecular pathogenesis, but also provides a detailed examination of each of the major methodologies needed to detect and analyze its associated genetic abnormalities.

Molecular Genetic Testing in Surgical Pathology

Molecular Genetic Testing in Surgical Pathology Book
Author : John D. Pfeifer
Publisher : Lippincott Williams & Wilkins
Release : 2006
ISBN : 9780781747486
Language : En, Es, Fr & De

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Book Description :

Written by experts from Washington University School of Medicine, this text is a thorough review of the specific molecular genetic techniques that can provide diagnostically useful molecular genetic information on tissue samples—including cytogenetics, fluorescence in situ hybridization (FISH), PCR, electrophoresis and hybridization analysis, DNA sequence analysis, and microarrays. The first part of the book describes each technique, indicates its advantages, disadvantages, capabilities, and limitations, and systematically addresses sensitivity and specificity issues. Subsequent chapters, organized by organ system, detail the specific applications of these tests in surgical pathology. More than 150 full-color and black-and-white illustrations complement the text.

Surgical Pathology Update 2001

Surgical Pathology Update 2001 Book
Author : Steffen Hauptmann,Manfred Dietel,Manuel Sobrinho-Simões
Publisher : Unknown
Release : 2001
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Surgical Pathology Update 2001 book written by Steffen Hauptmann,Manfred Dietel,Manuel Sobrinho-Simões, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

In Situ Hybridization

In Situ Hybridization Book
Author : James O'Donnell
Publisher : Oxford University Press, USA
Release : 1990
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Molecular biology and genetic engineering techniques have revolutionized our concepts of gene activity in cellular function. In situ hybridization has contributed to this revolution. It is the only technique which gives precise data on the content and distribution of DNA and mRNA in intactcells and tissues. It is now an invaluable test in understanding the pathophysiology of cellular functions. This book covers all aspects of in situ hybridization. It describes practical procedures and protocols, the scientific background, areas of application, and the limitations of the technology. The contributing authors are all experts. Each chapter contains up-to-date references, many colourillustrations, the latest advances, and prospects for the future. This practical guide will be invaluable to: molecular, cell, and developmental biologists; cyto- and molecular geneticists; clinicians, especially pathologists, biologists, and microbiologists; and scientists seriously interested inthe pathophysiology of cell function and its regulation.

Acta Histochemica Et Cytochemica

Acta Histochemica Et Cytochemica Book
Author : Anonim
Publisher : Unknown
Release : 2002
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Acta Histochemica Et Cytochemica book written by , available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Morphology Methods

Morphology Methods Book
Author : Ricardo V. Lloyd
Publisher : Springer Science & Business Media
Release : 2001-06-15
ISBN : 1592591906
Language : En, Es, Fr & De

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Book Description :

The past several decades have witnessed an impressive array of conceptual and techno logical advances in the biomedical sciences. Much of the progress in this area has developed directly as a result of new morphology-based methods that have permitted the assessment of chemical, enzymatic, immunological, and molecular parameters at the cellular and tissue levels. Additional novel approaches including laser capture microdissection have also emerged for the acquisition of homogeneous cell popula tions for molecular analyses. These methodologies have literally reshaped the approaches to fundamental biological questions and have also had a major impact in the area of diagnostic pathology. Much of the groundwork for the development of morphological methods was estab th lished in the early part of the 19 century by Francois-Vincent Raspail, generally acknowledged as the founder of the science of histochemistry. The earliest work in the field was primarily in the hands of botanists and many of the approaches to the under standing of the chemical composition of cells and tissues involved techniques such as microincineration, which destroyed structural integrity. The development of aniline th dyes in the early 20 century served as a major impetus to studies of the structural rather than chemical composition of tissue. Later in the century, however, the focus returned to the identification of chemical constituents in the context of intact cell and tissue structure.

Annual report

Annual report Book
Author : National Cancer Institute (U.S.). Division of Cancer Biology, Diagnosis, and Centers
Publisher : Unknown
Release : 1994
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Annual report book written by National Cancer Institute (U.S.). Division of Cancer Biology, Diagnosis, and Centers, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Anticancer Research

Anticancer Research Book
Author : Anonim
Publisher : Unknown
Release : 2003
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

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Science Culture

Science   Culture Book
Author : Anonim
Publisher : Unknown
Release : 2004
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Science Culture book written by , available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.