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Immunosensing For Detection Of Protein Biomarkers

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Immunosensing for Detection of Protein Biomarkers

Immunosensing for Detection of Protein Biomarkers Book
Author : Huangxian Ju,Guosong Lai,Feng Yan
Publisher : Elsevier
Release : 2017-05-18
ISBN : 0081020007
Language : En, Es, Fr & De

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Book Description :

Immunosensing for Detection of Protein Biomarkers not only introduces the principles, methods, and classification of immunoassay, but also presents the latest achievements in areas such as electrochemical immunosensors, nanoprobe-based immunoassay, chemiluminescence immunoassay, electrochemiluminescent immunoassay, multianalyte immunoassay, optical imaging for immunoassay, signal amplification for immunoassay, and so on. In recent years, immunosensing and immunoassay methods have attracted considerable interest due to their applications in different fields, particularly clinical diagnosis. Although a large number of academic papers in immunosensing and immunoassay have been published in different journals recently, it is still a difficult and time-consuming task for researchers, especially those new to the area, to understand the principles, methods, and research progress of immunosensing. Based on the research experience of the authors and their research groups, this book offers readers with new research ideas to develop immunosensing methodology. As a monograph, it offers deeper and more complete coverage than review papers, which only report certain aspects of progress. Grounded in the research experience of Professor Ju’s research group, the book focuses on immunosensing for detection of protein biomarkers, summarizing understanding, research, and practice on immunosensing methodology in detection of protein biomarkers. Presents the latest research and thinking on immunosensing for detection of protein biomarkers Offers current techniques, and looks to the development of new methodologies Offers the latest developments in various aspects of immunosensing, including electrochemiluminescent immunoassay, multianalyte immunoassay, optical imaging immunoassay, and signal amplification immunoassay Offers readers new ideas to research and develop immunosensing methodologies for the future

Development of Amperometric Immunosensors for Protein Biomarkers Using Single Wall Carbon Nanotube Forest and Electrochemical Detection of Enzyme Labels

Development of Amperometric Immunosensors for Protein Biomarkers Using Single Wall Carbon Nanotube Forest and Electrochemical Detection of Enzyme Labels Book
Author : Xin Yu
Publisher : Unknown
Release : 2006
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Development of Amperometric Immunosensors for Protein Biomarkers Using Single Wall Carbon Nanotube Forest and Electrochemical Detection of Enzyme Labels book written by Xin Yu, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Electrochemical Detection of Multiple Cancer Protein Biomarkers Using Microfluidic Device

Electrochemical Detection of Multiple Cancer Protein Biomarkers Using Microfluidic Device Book
Author : Bhaskara V. Chikkaveeraiah
Publisher : Unknown
Release : 2011
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Electrochemical Detection of Multiple Cancer Protein Biomarkers Using Microfluidic Device book written by Bhaskara V. Chikkaveeraiah, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Advanced Biosensors for Health Care Applications

Advanced Biosensors for Health Care Applications Book
Author : Dr. Inamuddin,Raju Khan,Ali Mohammad,Abdullah M. Asiri
Publisher : Elsevier
Release : 2019-06-15
ISBN : 0128165227
Language : En, Es, Fr & De

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Book Description :

Advanced Biosensors for Health Care Applications highlights the different types of prognostic and diagnostic biomarkers associated with cancer, diabetes, Alzheimer's disease, brain and retinal diseases, cardiovascular diseases, bacterial infections, as well as various types of electrochemical biosensor techniques used for early detection of the potential biomarkers of these diseases. Many advanced nanomaterials have attracted intense interests with their unique optical and electrical properties, high stability, and good biocompatibility. Based on these properties, advanced nanoparticles have been used as biomolecular carriers, signal producers, and signal amplifiers in biosensor design. Recent studies reported that there are several diagnostic methods available, but the major issue is the sensitivity and selectivity of these approaches. This book outlines the need of novel strategies for developing new systems to retrieve health information of patients in real time. It explores the potential of nano-multidisciplinary science in the design and development of smart sensing technology using micro-nanoelectrodes, novel sensing materials, integration with MEMS, miniaturized transduction systems, novel sensing strategy, that is, FET, CMOS, System-on-a-Chip (SoC), Diagnostic-on-a-Chip (DoC), and Lab-on-a-Chip (LOC), for diagnostics and personalized health-care monitoring. It is a useful handbook for specialists in biotechnology and biochemical engineering. Describes advanced nanomaterials for biosensor applications Relates the properties of available nanomaterials to specific biomarkers applications Includes diagnosis and electrochemical studies based on biosensors Explores the potential of nano-multidisciplinary science to design and develop smart sensing technologies Describes novel strategies for developing a new class of assay systems to retrieve the desired health information

Fabrication of Immunosensor Microwell Arrays from Gold Compact Discs for Detection of Cancer Biomarker Proteins

Fabrication of Immunosensor Microwell Arrays from Gold Compact Discs for Detection of Cancer Biomarker Proteins Book
Author : Chi Kin Tang
Publisher : Unknown
Release : 2013
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Fabrication of Immunosensor Microwell Arrays from Gold Compact Discs for Detection of Cancer Biomarker Proteins book written by Chi Kin Tang, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

A Multiplexing Immunosensor for the Quantification of Cytokine Biomarkers

A Multiplexing Immunosensor for the Quantification of Cytokine Biomarkers Book
Author : Aaron Fairchild
Publisher : Unknown
Release : 2012
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Biosensors offer excellent diagnostic methods through precise quantification of bodily fluid biomarkers and could fill an important niche in diagnostic screening. The long term goal of this research is the development of an impedance immunosensor for easy-to-use, rapid, sensitive and selective simultaneously multiplexed quantification of bodily fluid disease biomarkers. To test the hypothesis that various cytokines induce empirically determinable response frequencies when captured by printed circuit board (PCB) impedance immunosensor surface, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) methods were used to test PCB biosensors versus multiple cytokine biomarkers to determine limits of detection, background interaction and response at all sweep frequencies. Results indicated that sensors for cytokine Interleukin-12 (IL-12) detected their target over three decades of concentration and were tolerant to high levels of background protein. Further, the hypothesis that cytokine analytes may be rapidly detected via constant frequency impedance immunosensing without sacrificing undue sensitivity, CV, EIS, impedance-time (Zt) methods and modeling were used to test CHITM gold electrodes versus IL-12 over different lengths of time to determine limits of detection, detection time, frequency of response and consistent cross-platform sensor performance. Modeling and Zt studies indicate interrogation of the electrode with optimum frequency could be used for detection of different target concentrations within 90 seconds of sensor exposure and that interrogating the immunosensor with fixed, optimum frequency could be used for sensing target antigen. This informs usability of fixed-frequency impedance methods for biosensor research and particularly for clinical biosensor use. Finally, a multiplexing impedance immunosensor prototype for quantification of biomarkers in various body fluids was designed for increased automation of sample handling and testing. This enables variability due to exogenous factors and increased rapidity of assay with eased sensor fabrication. Methods were provided for simultaneous multiplexing through multisine perturbation of a sensor, and subsequent data processing. This demonstrated ways to observe multiple types of antibody-antigen affinity binding events in real time, reducing the number of sensors and target sample used in the detection and quantification of multiple biomarkers. These features would also improve the suitability of the sensor for clinical multiplex detection of disease biomarkers.

Ultrasensitive Arrays for Multiplexed Detection of Oral Cancer Serum Biomarker Proteins in the Clinic

Ultrasensitive Arrays for Multiplexed Detection of Oral Cancer Serum Biomarker Proteins in the Clinic Book
Author : Ruchika Malhotra
Publisher : Unknown
Release : 2012
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

The occurrence of cancer involves altered protein expression, known as biomarkers of disease. The serum levels of biomarker proteins are used as indicators to differentiate between diseased and healthy states, and to monitor disease progression. Sensitive measurement of proteins over-expressed in individuals with cancer holds excellent promise for early disease detection and personalized therapies. However, the required point-of-care measurement has yet to be broadly realized. Thus, there is an urgent need for simpler, faster and inexpensive detection of serum biomarkers with high sensitivity and accuracy. In this dissertation, new methodologies for ultrasensitive multiplexed detection of serum biomarker proteins for oral cancer, as well as other malignancies, utilizing different aspects of nanotechnology in conjunction with electrochemical detection have been developed and characterized. The first part of the approach addresses the study of interleukin-6 (IL-6) using single-walled carbon nanotube forests based electrochemical immunosensor on pyrolytic graphite (PG) electrodes. With two levels of multi-enzyme labeling in a sandwich immunoassay format, 25 fM human IL-6 in serum, and experimental samples, was reproducibly measured. The second part features 8-sensor microfluidic array equipped with four specific capture antibodies to detect ultralow levels of interleukin-6 (IL-6), interleukin-8 (IL-8), vascular endothelial growth factor (VEGF) and vascular endothelial growth factor C (VEGF-C) proteins, and these proteins when captured off-line using magnetic beads derivatized with 120,000 antibodies and 400,000 enzyme labels, greatly decreased non-specific binding and enhanced sensitivity. This approach provided unprecedented detection limits in low fg mL−1 range for all four biomarkers in calf serum, essentially used as a human serum surrogate. This oral cancer biomarker panel was validated by detecting proteins in sera of oral cancer patients and cancer-free controls. Receiver operating characteristic (ROC) analysis showed that a normalized combination of the levels of all 4 biomarkers enhanced the clinical discrimination of oral cancer from non-cancer conditions compared to single biomarkers, with 89% sensitivity and 98% specificity. The use of microfluidic immunoarrays for multiplexed detection of biomarker proteins offers distinct advantages. The capability and versatility of these methodologies for simultaneous, sensitive, accurate, low cost real-time monitoring of protein biomarkers with high clinical selectivity and sensitivity holds excellent promise for diagnosis and prognosis of cancer with potential for rationalizing treatment options. Also described in this dissertation is the development of electrochemical sensors for peanut allergen Ara h2 in serum, along with various analytical techniques to characterize the sensors to establish the primary reasons for high sensitivity and selectivity.

Programmable Bio nano chip Immunosensor for Multiplexed Detection of Ovarian Cancer Biomarkers

Programmable Bio nano chip Immunosensor for Multiplexed Detection of Ovarian Cancer Biomarkers Book
Author : Archana Raamanathan
Publisher : Unknown
Release : 2011
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Ovarian cancer is a high mortality disease where early stage detection may have significant survival benefits. Promising next-generation non-invasive, biomarker-based screening modalities involve longitudinal monitoring of serum biomarkers and multi-marker panel detection. Here, rapid, sensitive, precise and multiplexable diagnostic platforms can facilitate biomarker validation along with early detection and screening, and this work attempts to exploit the programmable bio-nano-chip (p-BNC) immunosensor to address these specific translational needs in ovarian cancer. First, the p-BNC was adapted for Cancer Antigen 125 (CA125) quantitation, the current FDA standard, with prominent implications in novel early detection and screening modalities. Antibody pairs binding to distinct epitopes on CA125 were identified and the p-BNC operating variables (incubation times, flow rates and reagent concentrations) were attuned to deliver optimal analytical performance (inter- and intra-assay precision of 1.2% and 1.9% and Limit-of-Detection (LOD) 1.0 U/mL), competitive with current gold standards, but with a short analysis time of 43 minutes. Further validation of the system with advanced stage patient sera (n=20) demonstrated good correlation with 'gold standard' ELISA (R2 = 0.97). Next, the p-BNC was adapted for concomitant analysis of CA125 and Human Epididymis Protein 4 (HE4), a novel multiplexed biomarker panel for early detection and screening. The HE4 immunoassay was developed to perform optimally with the 'rate determining' CA125 assay. Cross-reactivity analysis demonstrated high specificity multiplexing. The dose-response curves for the multiplexed CA125 and HE4 immunoassays were congruous with their singleplex counterparts with respective LODs of 0.51 U/mL and 4.18 pM and a total analysis time of 44 minutes. A small pilot scale clinical study was conducted to discriminate between surgically confirmed patient sera (n=8) and corresponding age-matched healthy controls (n=8) utilizing the multiplexed p-BNC, interpreted with a risk of ovarian malignancy algorithm. Successful discrimination was achieved between the groups with Receiver Operating Characteristic (ROC) curve AUC (Area Under the Curve) values of 1.00, 0.984 and 1.00 respectively for CA125, HE4 and the composite marker combination. Taken together, the analytical and clinical performance, multiplexing capabilities and the short turn-around times on the p-BNC offer methodological advancements over current gold standard techniques, indicating strong promise for ovarian cancer diagnostics.

Biomarkers of Human Exposure to Pesticides

Biomarkers of Human Exposure to Pesticides Book
Author : Mahmoud A. Saleh,Jerry N. Blancato,Charles H. Nauman,American Chemical Society. Division of Agrochemicals,American Chemical Society. Meeting
Publisher : Amer Chemical Society
Release : 1994
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Explores the role of biomarker data in evaluating the impact of human environmental and occupational exposure to pesticides. Focuses on two types of biomarkers: residue analysis of parent compounds or metabolites and end points representing interactions between xenobiotic and endogenous components. Characterizes the molecular basis of various biomarker methods and examines structure-activity relationships. Surveys biomarker measurement methods currently in use and examines methods for biomarker data analysis and applications of these methods to risk assessment.

Dissertation Abstracts International

Dissertation Abstracts International Book
Author : Anonim
Publisher : Unknown
Release : 2008
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Dissertation Abstracts International book written by , available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Chemical Abstracts

Chemical Abstracts Book
Author : Anonim
Publisher : Unknown
Release : 2002
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Chemical Abstracts book written by , available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Electrocatalytic Nanoparticle Based Sensing for Diagnostics

Electrocatalytic Nanoparticle Based Sensing for Diagnostics Book
Author : Marisa Maltez da Costa
Publisher : Unknown
Release : 2012
ISBN : 9788449033438
Language : En, Es, Fr & De

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Book Description :

An early and accurate diagnosis is the key to the effective and ultimately successful treatment of a large number of diseases, and only sensitive detection methods allow an early diagnosis. Current methods, employed in the clinical area, are often time-consuming, expensive, and require advanced instrumentation and high skilled professionals. Thus, more cost effective methods requiring user-friendly instrumentation that can provide an adequate sensitivity and accuracy would be ideal, and the most important challenge in biosensing is to combine the advances in nanomaterials and molecular biology, with new diagnosis methods in order to overcome the diagnosis difficulties Electrochemical biosensors can fulfil these requirements once they gather the selective biochemical recognition with the high sensitivity of electrochemical detection plus, they can be easily integrated in fluidic systems that enhance their overall manageability. To improve the electrochemical assay sensitivity and to achieve a better and more reliable analysis there is a great demand for labels with higher specific activity. The most used labels for electrochemical sensors up to date have been enzymes as well as small molecules like electro-active indicators. Nanoparticles can provide a novel platform for improving the specific activity of a label as well as its affinity to the tracer biomolecules (DNA probes, proteins and other biomolecules). They are within the same size range as biomolecules and in solution they present a similar behaviour. Therefore they can be used as electrochemical labels allowing more assay-flexibility, faster binding kinetics, high sensitivity and high reaction rates for many types of assays, ranging from protein immunoassays to DNA and cell analysis. The main objective of this thesis is the development of novel and improved electrochemical sensing systems for biomarker detection, using the electrocatalytic effects of nanoparticles. Several approaches were developed using gold nanoparticles as electrocatalytic labels in immunosensor and cell sensing systems, for the detection of proteins and cells with interest for the detection of biomarkers.

Electrochemical Immunosensors and Aptasensors

Electrochemical Immunosensors and Aptasensors Book
Author : Paolo Ugo,Ligia M. Moretto
Publisher : MDPI
Release : 2018-03-23
ISBN : 3038424064
Language : En, Es, Fr & De

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Book Description :

This book is a printed edition of the Special Issue "Electrochemical Immunosensors and Aptasensors" that was published in Chemosensors

American Biotechnology Laboratory

American Biotechnology Laboratory Book
Author : Anonim
Publisher : Unknown
Release : 2008
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download American Biotechnology Laboratory book written by , available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Carbon Nanotube Biosensors for Detection of Biomarkers in Breast Cancer Cells

Carbon Nanotube Biosensors for Detection of Biomarkers in Breast Cancer Cells Book
Author : Benjamin C. King
Publisher : Unknown
Release : 2012
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Detection and profiling of circulating tumor cells (CTCs) is useful for cancer screening and for managing treatment of carcinoma patients. Label-free technologies aim to accomplish detection rapidly with small, simple micro and nano devices. Carbon nanotubes are favorable molecular sensors due to their unique properties. They have been widely investigated for immunosensing of cancer biomarkers as free proteins, but very little has been done to detect biomarkers in intact cells and much remains to be understood regarding the mechanism of their sensing. We have developed a simple carbon nanotube biosensor for epithelial cell adhesion molecule (EpCAM) for sensing EpCAM positive cells. Sensor fabrication steps involving minimal air exposure were employed which reduced random noise upon sample introduction to the device. Optimized sensors recognized specific interactions with EpCAM positive MCF-7 cells and did not recognize EpCAM negative MCF-10A cells, producing the same characteristic signal as for blank phosphate buffered saline samples (no interaction). A two sample t-test found that the specific and nonspecific signals were significantly different, p = 0.0235. Specific binding signals are attributed to the combined binding events and negative cell membrane potential.

Detection of Tau Biomarkers Using Tau Specific Antibodies Immobilized on a Gold Surface

Detection of Tau Biomarkers Using Tau Specific Antibodies Immobilized on a Gold Surface Book
Author : Anonim
Publisher : Unknown
Release : 2015
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Tau protein belongs to a class of microtubule-binding proteins (MAP) which all originate from the common MAPT gene located on chromosome (21). Tau proteins are primarily found in neurons, where they interact with microtubules and serve to stabilize microtubules in neural axons. The malfunction of tau is one of the major causes of Alzheimer's Disease (AD), however, the early detection and treatment of AD are still elusive. The detection of tau species and their levels was achieved through the use of electrochemical immunoassay based on anti-tau antibodies immobilized on a gold surface for potential use in real-time detection of tau as a biomarker for AD. The anti-tau antibodies were bound to a carboxylic acid-modified gold surface, and EIS was used to characterize the affinity of antibody for full-length tau (Tau-441). The modulation in the electrochemical signal was due to antibody binding to tau with high specificity and selectivity. The electrochemical immunosensor did not significantly respond with a sensor surface absent of detecting antibodies, or with the non-tau-specific antibody MOPC-21. The electroimmunosensor was tested to determine dynamic range of detection, and compared to the ELISA and PCR methodologies.

Genetic Engineering Biotechnology News

Genetic Engineering   Biotechnology News Book
Author : Anonim
Publisher : Unknown
Release : 2008
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Genetic Engineering Biotechnology News book written by , available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Science

Science Book
Author : John Michels
Publisher : Unknown
Release : 2008
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Since Jan. 1901 the official proceedings and most of the papers of the American Association for the Advancement of Science have been included in Science.

Cancer Research

Cancer Research Book
Author : Anonim
Publisher : Unknown
Release : 2009
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Cancer Research book written by , available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.