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Genome Engineering Via Crispr Cas9 System

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Genome Engineering via CRISPR Cas9 System

Genome Engineering via CRISPR Cas9 System Book
Author : Vijai Singh,Pawan K. Dhar
Publisher : Academic Press
Release : 2020-02-18
ISBN : 0128181419
Language : En, Es, Fr & De

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Book Description :

Genome Engineering via CRISPR-Cas9 Systems presents a compilation of chapters from eminent scientists from across the globe who have established expertise in working with CRISPR-Cas9 systems. Currently, targeted genome engineering is a key technology for basic science, biomedical and industrial applications due to the relative simplicity to which they can be designed, used and applied. However, it is not easy to find relevant information gathered in a single source. The book contains a wide range of applications of CRISPR in research of bacteria, virus, algae, plant and mammalian and also discusses the modeling of drosophila, zebra fish and protozoan, among others. Other topics covered include diagnosis, sensor and therapeutic applications, as well as ethical and regulatory issues. This book is a valuable source not only for beginners in genome engineering, but also researchers, clinicians, stakeholders, policy makers, and practitioners interested in the potential of CRISPR-Cas9 in several fields. Provides basic understanding and a clear picture on how to design, use and implement the CRISPR-Cas9 system in different organisms Explains how to create an animal model for disease research and screening purposes using CRISPR Discusses the application of CRISPR-Cas9 systems in basic sciences, biomedicine, virology, bacteriology, molecular biology, neurology, cancer, industry, and many more

Reprogramming the Genome Applications of CRISPR Cas in non mammalian systems part B

Reprogramming the Genome  Applications of CRISPR Cas in non mammalian systems part B Book
Author : Anonim
Publisher : Academic Press
Release : 2021-04-29
ISBN : 032385298X
Language : En, Es, Fr & De

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Book Description :

Reprogramming the Genome: Applications of CRISPR-Cas in Non-mammalian Systems Part B, represents the collation of chapters written by eminent scientists worldwide. CRISPR-Cas9 system is an RNA-mediated immune system of bacteria and archaea that protects from bacteriophage infections. It is one of the revolutionized technologies to uplift biology to the next stages. It is a simple, rapid, precise, and cost-effective tool for genome editing and regulation of a wide range of organisms. It has gained scientific and public attention worldwide. This volume mainly covers insect cell line, protozoans, zebrafish, drosophila, CRISPRi, patents as well as technology transfer, and many more. This book is a key source of information available in a single volume. This book will be useful for not only beginners in genome engineering, but also students, researchers, scientists, policymakers, and stakeholders interested in harnessing the potential of reprogramming of the genomes in several areas. Offers basic understanding and a clear picture of genome editing CRISPR-Cas systems in different organisms Explains how to create an animal model for disease diagnosis/research and reprogram CRISPR for insect cell line, protozoans, zebrafish, drosophila, and many more Discusses the advances, patents, applications, challenges and opportunities in CRISPR-Cas9 systems in basic sciences, biomedicine, molecular biology and many more

Genome editing technologies and their impact

Genome editing technologies and their impact Book
Author : Institut d'Estudis Catalans
Publisher : Institut d'Estudis Catalans
Release : 2020-11-26
ISBN : 8499655645
Language : En, Es, Fr & De

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Book Description :

Download Genome editing technologies and their impact book written by Institut d'Estudis Catalans, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Abiotic Stress Signaling in Plants Functional Genomic Intervention

Abiotic Stress Signaling in Plants  Functional Genomic Intervention Book
Author : Girdhar K. Pandey,Manoj Prasad,Amita Pandey,Maik Boehmer
Publisher : Frontiers Media SA
Release : 2016-08-08
ISBN : 288919891X
Language : En, Es, Fr & De

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Book Description :

Abiotic stresses such as high temperature, low-temperature, drought and salinity limit crop productivity worldwide. Understanding plant responses to these stresses is essential for rational engineering of crop plants. In Arabidopsis, the signal transduction pathways for abiotic stresses, light, several phytohormones and pathogenesis have been elucidated. A significant portion of plant genomes (Arabidopsis and rice were mostly studied) encodes for proteins involves in signaling such as receptor, sensors, kinases, phosphatases, transcription factors and transporters/channels. Despite decades of physiological and molecular effort, knowledge pertaining to how plants sense and transduce low and high temperature, low-water availability (drought), water-submergence, microgravity and salinity signals is still a major question for plant biologist. One major constraint hampering our understanding of these signal transduction processes in plants has been the lack or slow pace of application of molecular genomic and genetics knowledge in the form of gene function. In the post-genomic era, one of the major challenges is investigation and understanding of multiple genes and gene families regulating a particular physiological and developmental aspect of plant life cycle. One of the important physiological processes is regulation of stress response, which leads to adaptation or adjustment in response to adverse stimuli. With the holistic understanding of the signaling pathways involving not only one gene family but multiple genes or gene families, plant biologist can lay a foundation for designing and generating future crops, which can withstand the higher degree of environmental stresses (especially abiotic stresses, which are the major cause of crop loss throughout the world) without losing crop yield and productivity. Therefore, in this e-Book, we intend to incorporate the contribution from leading plant biologists to elucidate several aspects of stress signaling by functional genomics approaches.

Biopolymer Based Formulations

Biopolymer Based Formulations Book
Author : Dr. Kunal Pal,Indranil Banerjee,Preetam Sarkar,Doman Kim,Win-Ping Deng,Navneet Kumar Dubey,Kaustav Majumder
Publisher : Elsevier
Release : 2020-01-18
ISBN : 0128168986
Language : En, Es, Fr & De

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Book Description :

Biopolymer-Based Formulations: Biomedical and Food Applications presents the latest advances in the synthesis and characterization of advanced biopolymeric formulations and their state-of-the-art applications across biomedicine and food science. Sections cover the fundamentals, applications, future trends, environmental, ethical and medical considerations, and biopolymeric architectures that are organized in nano, micro and macro scales. The final section of the book focuses on novel applications and recent developments. This book is an essential resource for researchers, scientists and advanced students in biopolymer science, polymer science, polymer chemistry, polymer composites, plastics engineering, biomaterials, materials science, biomedical engineering, and more. It will also be of interest to R&D professionals, scientists and engineers across the plastics, food, biomedical and pharmaceutical industries. Provides in-depth coverage of methods for the characterization of the physical properties of biopolymeric architectures Supports a range of novel applications, including scaffolds, implant coatings, drug delivery, and nutraceutical encapsulation systems Includes the use of experimental data and mathematical modeling, thus enabling the reader to analyze and compare the properties of different polymeric gels

Development and Application of Novel Genome Engineering Tools in Microbial Biotechnology

Development and Application of Novel Genome Engineering Tools in Microbial Biotechnology Book
Author : Jiazhang Lian,Yi Wang,Yunzi Luo,Chun Li
Publisher : Frontiers Media SA
Release : 2021-01-20
ISBN : 2889664058
Language : En, Es, Fr & De

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Book Description :

Download Development and Application of Novel Genome Engineering Tools in Microbial Biotechnology book written by Jiazhang Lian,Yi Wang,Yunzi Luo,Chun Li, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

SCHWARTZ S PRINCIPLES OF SURGERY 2 volume set 11th edition

SCHWARTZ S PRINCIPLES OF SURGERY 2 volume set 11th edition Book
Author : F. Charles Brunicardi,Dana K. Andersen,Timothy R. Billiar,David L. Dunn,John G. Hunter,Lillian Kao,Jeffrey B. Matthews,Raphael E. Pollock
Publisher : McGraw Hill Professional
Release : 2019-05-29
ISBN : 1259835375
Language : En, Es, Fr & De

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Book Description :

Publisher's Note: Products purchased from Third Party sellers are not guaranteed by the publisher for quality, authenticity, or access to any online entitlements included with the product. The BEST EDITION yet of the #1 text for surgical practice and education For half-a-century, no other text has provided such a solid grounding in basic science, anatomy, operative techniques, and more recently, professional development and leadership training, as Schwartz’s Principles of Surgery. Written by the world’s foremost surgeons, this landmark reference offers distinctly modern and all-encompassing coverage of every important topic in general surgery. Enhanced by a new two volume presentation, the Eleventh Edition has been completely updated and refreshed with an emphasis on state-of-the-art, evidence-based surgical care. You will find an exciting array of new contributors from around the world, new chapters on cutting-edge topics, plus the acclaimed learning aids that make the material easier to understand and memorize. This outstanding content is bolstered by more than 800 photographs and 1,300 line drawings, most in full color, as well as online videos demonstrating key operations. Here’s why the Eleventh Edition is the best edition yet: Six timely new chapters on important topics such as enhanced recovery after surgery (ERAS), ambulatory/outpatient surgery, evidence for surgery practice, skills and simulation, and web-based education and social media High-quality full-color design showcases an unsurpassed illustration program Emphasis on high-yield discussion of diagnosis and treatment of surgical disease, arranged by organ system and surgical specialty Acclaimed learning aids (many new to this edition), including an abundance of completely up-to-date tables that summarize the most current evidence, boxed key points, detailed anatomical figures, diagnostic and management algorithms, and an abundance of completely up-to-date tables, and key references More than the field’s cornerstone textbook, Schwartz’s Principles of Surgery is an international compendium of the knowledge and technique of the world’s leading surgeons.

Zwitterlation Improves Efficacy and Safety of Therapeutic Proteins

Zwitterlation Improves Efficacy and Safety of Therapeutic Proteins Book
Author : Yanjiao Han
Publisher : Unknown
Release : 2020
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

During the past decades, engineered proteins have been widely used as biopharmaceutical drugs (e.g., recombinant therapeutic proteins, enzymes, and monoclonal antibodies) and gene editing tools (e.g., zinc fingers, TALENs and CRISPR/Cas9). This has significantly transformed the pharmaceutical industry. Compared with conventional small molecule drugs, these biomacromolecules offer the advantages of higher specificity and potency. However, safety and efficacy concerns have been raised from different aspects. In this thesis, the author discusses the tactics to improve efficacy and safety of protein therapeutics. First, to protect the protein from body clearance and immune system, a poly(carboxybetaine) (pCB) polymer is conjugated to the protein surface. Compared with PEG conjugation, the zwitterionic coating more effectively shields protein epitopes, rendering the whole protein invisible to the body immune system. This strategy is proved to be effective in increasing protein stability, prolonging in vivo circulation half-life and reducing immunogenicity, and is successfully applied to interferon-alpha (IFN-alpha). Compared with PEGylated IFN-alpha, Zwitterlated IFN-alpha largely retains its in vitro efficacy. In addition, the author further discusses the tactics to improve the safety and efficacy of nuclease for gene editing. To reduce the off-target effect of CRISPR/Cas9 system, the author discusses a novel method that conjugating CRISPR/Cas9 with zwitterionic pCB polymer to address the mismatch issue at the off-target sites. The author demonstrates the conjugation of CRISPR/Cas9 system with pCB polymer can both increase the efficacy of Cas9 nuclease and reduce non-specific interactions between gRNA and DNA (off-target DNA break). Finally, we converted the strategy to the mRNA version via a new strategy for reducing off-target effect of CRISPR/Cas9 systems that relies on fusing zwitterionic peptide poly(EK) extensions to the terminus of Cas9 via genetic engineering. The poly(EK) peptide is biodegradable and potentially safer for clinical applications. This technology was realized through mRNA delivery and will provide a robust and easily used strategy to reduce "off-target" mutations on a genome-wide scale and to improve the efficiency of a wide range of CRISPR/Cas9-based biological and clinical applications.

Engineering Agrobacterium Tumefaciens to Facilitate and Improve Knock in Efficiency in Plants Via Cas9 Editing Strategies

Engineering Agrobacterium Tumefaciens to Facilitate and Improve Knock in Efficiency in Plants Via Cas9 Editing Strategies Book
Author : Adam John Kirby
Publisher : Unknown
Release : 2020
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

The development of efficient tools for plant genome engineering is key to dramatically expedite basic research approaches and, at the same time, facilitate translational and biotechnological strategies in agriculture to improve crops and meet future food demand. For decades, technologies enabling precise Gene Targeting (GT) and efficient DNA knock-in or sequence replacement via Homology-Directed Repair (HDR) in plants has remained challenging, and although some recent reports suggest that progress is being made, no efficient and reproducible protocols have yet been established. Agrobacterium tumefaciens-mediated insertion entails the mobilization of the T-DNA into the host plant genome. However, this process occurs randomly and the system is very inefficient for targeted knock-ins. For this project, we capitalize on the extensive knowledge of Agrobacterium tumefaciens biology and combine it with CRISPR-Cas9 genome editing technologies to develop a novel strategy for high efficiency targeted knock-in. To do so, we decided to engineer Agrobacterium tumefaciens to help us reduce the distance between the T-DNA (harboring the cassette for knock-in) and the Cas9 cut site that marks the location where specifically the targeted insertion will occur. The set of tools we are presenting here will serve not to only accelerate basic research but also assist in engineering crop genomes in order to meet our future food demand in an efficient and sustainable manner.

Genome Editing in Kenaf

Genome Editing in Kenaf Book
Author : Kangqi Li
Publisher : Unknown
Release : 2020
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

The potential for Hibiscus cannabinus L. (kenaf) improvement via genome editing using the CRISPR/Cas9 system to generate gene knock-outs was explored. Studies included target gene identification, target guide RNA (gRNA) selection, plant tissue (explant) choice and media composition for plant regeneration. A putative kenaf phytoene desaturase gene (pds, GEED01047592.1) was identified in the kenaf transcriptome, and molecularly confirmed. Kenaf seedling tissues were transformed via Agrobacterium tumefaciens containing the cas9 gene (endonuclease required for gene knock-out) and each gRNA separately; putative transgenic calli and adventitious shoots arose on a medium containing 1-naphthaleneacetic acid, thidiazuron and silver nitrate. Tissues appeared chlorotic/albino and shoots remained diminutive/dwarf-like. These unique morphologies had also been noted by researchers who successfully knocked out the pds gene in other plant species. Cas9 DNA was detected in these putative transgenic kenaf tissues, but initial DNA sequencing analysis did not confirm knock-out/mutations in targeted areas of the pds gene.

Cas9 Ribonucleoprotein Delivery Via Microfluidic Cell Deformation Chip for Human T Cell Genome Editing and Immunotherapy

Cas9 Ribonucleoprotein Delivery Via Microfluidic Cell   Deformation Chip for Human T   Cell Genome Editing and Immunotherapy Book
Author : Anonim
Publisher : Unknown
Release : 2017
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Abstract : This study reports a microfluidic cell deformation‐based method to deliver the Cas9 ribonucleoprotein (RNP) complexes to different cell types for efficient genome editing, including hard‐to‐transfect human primary CD4+ T cells. The RNP based CRISPR‐Cas9 system has great advantage in shortening reaction time and reducing off‐target problems, which holds great potential in future gene therapy applications.

Williams Hematology 10th Edition

Williams Hematology  10th Edition Book
Author : Kenneth Kaushansky,Marshall A. Lichtman,Josef T. Prchal,Marcel M. Levi,Linda J. Burns
Publisher : McGraw Hill Professional
Release : 2021-01-14
ISBN : 126046413X
Language : En, Es, Fr & De

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Book Description :

The landmark text that has guided generations of hematologists and related practitioners―updated with the latest research findings and improved format and presentation Long revered for its comprehensiveness and extraordinary depth of detail, Williams Hematology provides essential coverage of the origins, pathophysiological mechanisms, and management of benign and malignant disorders of blood and marrow cells and coagulation proteins. The text contains a wealth of basic science and translational pathophysiology for optimal, lifelong learning. Experts in research and clinical hematology, the editors are known worldwide for their contributions to the field. This new edition contains everything that has made Williams Hematology the go-to resource for decades and has been updated with new chapters and critical new research into the molecular mechanisms responsible for hematological disorders and the impact on diagnosis and treatment. And the new format enables you to access each chapter via content modules covering key topics, with summaries, infographics, and cases―all linked to review questions for self-assessment. The full-color presentation integrates images of blood and tissue findings where they are cited in the text. NEW TO THIS EDITION: Updated and revised content reflecting the latest research and developments Convenient format that streamlines the learning process and improves retention Additional chapters added on: Immune Checkpoint Inhibitors Immune Cell Therapy: Chimeric Antigen Receptor T Cell Therapy Immune Cell Therapy Dendritic Cell and Natural Killer Cell Therapy The processes of cell death and survival Application of Big Data and Deep Learning in Hematology Williams Hematology Cases with multiple-choice questions including detailed explanations—perfect preparation for the boards Continuously updated online content with comprehensive drug therapy database and other resources

Epigenetic Shaping of Sociosexual Interactions From Plants to Humans

Epigenetic Shaping of Sociosexual Interactions  From Plants to Humans Book
Author : Anonim
Publisher : Academic Press
Release : 2014-09-03
ISBN : 9780128002223
Language : En, Es, Fr & De

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Book Description :

Epigenetic Shaping of Sociosexual Interactions: From Plants to Humans is the first attempt to interpret the higher social functions of organisms. This volume covers an extraordinarily wide range of biological research and provides a novel framework for understanding human-specific brain functions. Covers an extraordinarily wide range of biological research Provides a novel framework for understanding human-specific brain functions.

Goodman and Gilman s The Pharmacological Basis of Therapeutics 13th Edition

Goodman and Gilman s The Pharmacological Basis of Therapeutics  13th Edition Book
Author : Laurence Brunton,Bjorn Knollman,Randa Hilal-Dandan
Publisher : McGraw Hill Professional
Release : 2017-10-26
ISBN : 1259584747
Language : En, Es, Fr & De

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Book Description :

The gold-standard of pharmacology texts – completely updated to reflect the latest research and developments A Doody’s Core Title for 2019! Goodman & Gilman’s: The Pharmacological Basis of Therapeutics, Thirteenth Edition represents the pinnacle of authority and accuracy in describing the actions and uses of therapeutic agents in relation to physiology and pathophysiology. Goodman & Gilman’s careful balance of basic science and clinical application has guided thousands of practitioners and students to a clear understanding of the drugs essential to preventing, diagnosing, and treating disease. The Thirteenth Edition includes more than 500 color illustrations, with many new figures emphasizing mechanisms of drug action. More than 30 new contributors have added to this edition, while the focus on basic principles is undiminished. This edition is enhanced by timely new content: •NEW chapters including Treatment of Pulmonary Arterial Hypertension, Immunity and Inflammation, Immunoglobulins and Vaccines, and Treatment of Viral Hepatitis •Expanded coverage of cardiovascular disease, with separate chapters on myocardial ischemia, hypertension, and heart failure •Increased emphasis on cellular signaling pathways involved in drug action •Summary tables at the end of each chapter that organize drugs discussed in that chapter into relevant categories and detail therapeutic usage, clinical pharmacology, and tips •Chapter Content Outlines at the beginning of each chapter •Abbreviation boxes in every chapter to easily identify the abbreviations appearing in that chapter More than a textbook, Goodman & Gilman’s is a working template for the effective and rational prescribing of drugs in daily practice.

Cell Culture Engineering

Cell Culture Engineering Book
Author : Gyun Min Lee,Helene Faustrup Kildegaard
Publisher : John Wiley & Sons
Release : 2020-01-13
ISBN : 3527343342
Language : En, Es, Fr & De

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Book Description :

Offers a comprehensive overview of cell culture engineering, providing insight into cell engineering, systems biology approaches and processing technology In Cell Culture Engineering: Recombinant Protein Production, editors Gyun Min Lee and Helene Faustrup Kildegaard assemble top class authors to present expert coverage of topics such as: cell line development for therapeutic protein production; development of a transient gene expression upstream platform; and CHO synthetic biology. They provide readers with everything they need to know about enhancing product and bioprocess attributes using genome-scale models of CHO metabolism; omics data and mammalian systems biotechnology; perfusion culture; and much more. This all-new, up-to-date reference covers all of the important aspects of cell culture engineering, including cell engineering, system biology approaches, and processing technology. It describes the challenges in cell line development and cell engineering, e.g. via gene editing tools like CRISPR/Cas9 and with the aim to engineer glycosylation patterns. Furthermore, it gives an overview about synthetic biology approaches applied to cell culture engineering and elaborates the use of CHO cells as common cell line for protein production. In addition, the book discusses the most important aspects of production processes, including cell culture media, batch, fed-batch, and perfusion processes as well as process analytical technology, quality by design, and scale down models. -Covers key elements of cell culture engineering applied to the production of recombinant proteins for therapeutic use -Focuses on mammalian and animal cells to help highlight synthetic and systems biology approaches to cell culture engineering, exemplified by the widely used CHO cell line -Part of the renowned "Advanced Biotechnology" book series Cell Culture Engineering: Recombinant Protein Production will appeal to biotechnologists, bioengineers, life scientists, chemical engineers, and PhD students in the life sciences.

Efficient and Multiplex Genome Editing Using GeneArt CRISPR Nuclease MRNA

Efficient and Multiplex Genome Editing Using GeneArt   CRISPR Nuclease MRNA Book
Author : Johan Garza,Jon Chesnut,Sanjay Kumar,Ravinder Namritha,Shantanu Kumar
Publisher : Unknown
Release : 2015
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system is derived from a prokaryotic adaptive immune system that uses an RNA-guided DNA nuclease to mutate and silence viral DNA infections (Jinek et al., 2012). These nucleases have been re-engineered to function as gene editing tools in various organisms, including mammalian cells (Cong et al., 2013). Thermo Fisher Scientific has developed the GeneArt® CRISPR Nuclease mRNA and in vitro transcribed gRNA from GeneArt® T7 string DNA that function together to efficiently and conveniently modify the genomic loci of choice, while circumventing promoter-associated restrictions, avoiding random DNA integrations from previous DNA vector-based solutions and show potential for in vivo applications. The goal of the Biotech Master's Semester In Residence (SIR) project is to demonstrate product and application data of GeneArt® CRISPR Nuclease mRNA (Cas9 mRNA) system coupled with in vitro transcribed (IVT) guide RNAs (gRNAs) showing efficient editing in a broad range of cell types including human and mouse derived cell lines, as well as induced pluripotent stem cells (iPSCs). Further, to demonstrate its capabilities, we used GeneArt® CRISPR nuclease mRNA and a set of gRNAs to simultaneously target several genomic loci at once in a multiplexed fashion and to result in clonal populations with mutations at chosen loci. Such clones were screened using the gel-based Genomic Cleavage Detection assay and confirmed through Sanger sequencing of PCR amplified targeted DNA fragments via targeted DNA via TOPO® TA Cloning kit. The report within demonstrates how the GeneArt® CRISPR nuclease mRNA (Catalog # A25640) can be used for performing CRISPR-Cas9 mediated genome editing. Coupled with IVT gRNA, where one can efficiently and conveniently modify their target genomic sequences. Because of eliminating promoter-associated constraints, the complete RNA format combined with RNA-specific transfection reagents, provides superior delivery combined with high genome editing efficiency in broad cell types including stem cells. GeneArt® CRISPR Nuclease mRNA system along with the complete work flow solutions described in the report can address both small and large scale cell engineering needs.

SaCas9 Requires 5 NNGRRT 3 PAM for Sufficient Cleavage and Possesses Higher Cleavage Activity Than SpCas9 Or FnCpf1 in Human Cells

SaCas9 Requires 5      NNGRRT   3    PAM for Sufficient Cleavage and Possesses Higher Cleavage Activity Than SpCas9 Or FnCpf1 in Human Cells Book
Author : Anonim
Publisher : Unknown
Release : 2018
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Abstract: CRISPR/Cas9‐mediated gene therapy holds great promise for the treatment of human diseases. The protospacer adjacent motif (PAM), the sequence adjacent to the target sequence, is an essential targeting component for the design of CRISPR/Cas9‐mediated gene editing. However, currently, very few studies have attempted to directly study the PAM sequence in human cells. To address this issue, the authors develop a dual fluorescence reporter system that could be harnessed for identifying functional PAMs for genome editing endonuclease, including Cas9. With this system, the authors investigate the effects of different PAM sequences for SaCas9, which is small and has the advantage of allowing in vivo genome editing, and found only 5′‐NNGRRT‐3′ PAM could induced sufficient target cleavage with multi‐sites. The authors also found SaCas9 possesses higher activity than SpCas9 or FnCpf1 via plasmids (episomal) and chromosomes with integrated eGFP‐based comparison. Taken together, the authors show that a dual fluorescence reporter system is a means to identifying a functional PAM and quantitatively comparing the efficiency of different genome editing endonucleases with the similar or identical target sequence in human cells. Abstract : Currently, very few studies have attempted to study the PAM sequence of CRISPR/Cas9 directly in human cells. To address this issue, the authors develop a dual fluorescence reporter system (pmT/mG), and found SaCas9 requires 5′‐NNGRRT‐3' for sufficient cleavage in human cells. The authors also found SaCas9 possesses higher activity than SpCas9 or FnCpf1 in human cells via plasmids (episomal) and chromosomes based comparison.

Harpers Illustrated Biochemistry 30th Edition

Harpers Illustrated Biochemistry 30th Edition Book
Author : Victor W. Rodwell,David Bender,Kathleen M. Botham,Peter J. Kennelly,P. Anthony Weil
Publisher : McGraw Hill Professional
Release : 2015-03-22
ISBN : 0071825371
Language : En, Es, Fr & De

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Book Description :

Gain a thorough understanding of the principles ofbiochemistry as they relate to the study of clinical medicine A Doody's Core Title for 2017! THE BEST REVIEW FOR THE USMLE! The Thirtieth Edition of Harper’s Illustrated Biochemistry combines outstanding full-colorillustrations with authoritative integrated coverage of biochemical disease and clinical information. Using brevity and numerous medically relevant examples, Harper's presents a clear, succinct review of the fundamentals of biochemistry that every student must understand in order to succeed in medical school. All fifty-eight chapters emphasize the medical relevance of biochemistry Full-color presentation includes more than 600 illustrations Each chapter includes a section on BiomedicalImportance and a summary of the topics covered Review questions follow each of the eleven sections Case studies in every chapter emphasize the clinical relevance to biochemistry NEW coverage of toxic naturally-occurring amino acids; extraterrestrial biomolecules; computer-aided drug design; the role of complement cascade in bacterial and viral infection; secreted mediators of cell-cell signaling between leukocytes; the role of mast cells, basophils, andeosinophils; and the hazard of antioxidants that down-regulate radical signaling for apoptosis and increase risk of cancer Applauded by medical students for its current and engaging style, Harper's Illustrated Biochemistry is an essential for USMLE review and the single best reference for learning the clinical relevance of any biochemistry topic.

First Aid for the USMLE Step 1 2018 28th Edition

First Aid for the USMLE Step 1 2018  28th Edition Book
Author : Tao Le,Vikas Bhushan,Matthew Sochat,Yash Chavda,Andrew Zureick
Publisher : McGraw Hill Professional
Release : 2017-12-21
ISBN : 1260116131
Language : En, Es, Fr & De

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Book Description :

Publisher's Note: Products purchased from Third Party sellers are not guaranteed by the publisher for quality, authenticity, or access to any online entitlements included with the product. SCORE YOUR HIGHEST ON THE USMLE® STEP 1 WITH HELP FROM THE WORLD’S MOST POPULAR MEDICAL REVIEW BOOK An unmatched collection of more than 1,300 must-know facts and mnemonics provide a complete framework for your USMLE® preparation and coursework! This annually updated review delivers a comprehensive collection of high-yield facts and mnemonics that pinpoint exactly what you need to know to pass the exam. Co-authored by medical students who recently took the boards, it provides a complete framework to help you prepare for the most stressful exam of your career. Here's why this is the ultimate USMLE Step 1 review:•A complete framework for USMLE® Step 1 preparation, annually updated with crowdsourced contributions from thousands of students •Written by students who excelled on their Step 1 exam and reviewed by top faculty for accuracy•1,300 must-know topics with mnemonics to focus your study•1,000+ color photos and illustrations – 100 new or revised -- help you visualize processes, disorders, and clinical findings•Student-proven study and exam prep tips backed by learning science•Reorganized Rapid Review section for efficient, last-minute preparation•Revised and expanded recommendations for high-yield print and digital study resources s•Bonus material and real-time updates exclusively at FirstAidTeam.com

Williams Hematology 9E

Williams Hematology  9E Book
Author : Kenneth Kaushansky,Marshall A. Lichtman,Josef Prchal,Marcel M. Levi,Oliver W Press,Linda J Burns,Michael Caligiuri
Publisher : McGraw Hill Professional
Release : 2015-12-23
ISBN : 0071833013
Language : En, Es, Fr & De

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Book Description :

Publisher's Note: Products purchased from Third Party sellers are not guaranteed by the publisher for quality, authenticity, or access to any online entitlements included with the product. The world's most highly regarded reference text on the mechanisms and clinical management of blood diseases A Doody's Core Title for 2019! Edition after edition, Williams Hematology has guided generations of clinicians, biomedical researchers, and trainees in many disciplines through the origins, pathophysiological mechanisms, and management of benign and malignant disorders of blood cells and coagulation proteins. It is acknowledged worldwide as the leading hematology resource, with editors who are internationally regarded for their research and clinical achievements and authors who are luminaries in their fields. The Ninth Edition of Williams Hematology is extensively revised to reflect the latest advancements in basic science, translational pathophysiology, and clinical practice. In addition to completely new chapters, it features a full-color presentation that includes 700 photographs, 300 of which are new to this edition, and 475 illustrations. Recognizing that blood and marrow cell morphology is at the heart of diagnostic hematology, informative color images of the relevant disease topics are conveniently integrated into each chapter, allowing easy access to illustrations of cell morphology important to diagnosis. Comprehensive in its depth and breath, this go-to textbook begins with the evaluation of the patient and progresses to the molecular and cellular underpinnings of normal and pathological hematology. Subsequent sections present disorders of the erythrocyte, granulocytes and monocytes, lymphocytes and plasma cells, malignant myeloid and lymphoid diseases, hemostasis and thrombosis, and transfusion medicine.