Genetic And Metabolic Engineering For Improved Biofuel Production From Lignocellulosic Biomass

Book Description :

Genetic and Metabolic Engineering for Improved Biofuel Production from Lignocellulosic Biomass describes the different aspects of biofuel production from lignocellulosic biomass. Each chapter presents different technological approaches for cost effective liquid biofuel production from agroresidues/biomass. Two chapters cover future direction and the possibilities of biomass-based biofuel production at the industrial level. The book provides a genetic and metabolic engineering approach for improved cellulase production and the potential of strains that can ferment both pentose and hexose sugars. The book also gives direction on how to overcome challenges for the further advancement of lignocellulosic biomass-based biofuel production. Covers genetic engineering approaches for higher cellulase production from fungi Includes genetic and metabolic engineering approaches for development of potential pentose and hexose fermenting strain which can tolerate high ethanol and toxic phenolic compounds Describe different bioreactors used in different steps of biomass-based biofuel production Outlines future prospects and potential of biofuel production from lignocellulosic biomass

Book Description :

Production of Top 12 Biochemicals Selected by USDOE from Renewable Resources: Status and Innovation covers all important technological aspects of the production of biochemicals from renewable feedstock. All the important technological aspects of biomass conversion for example biomass pretreatment, enzymatic hydrolysis for cellulosic sugars production followed by the fermentation into chemicals and downstream recovery of the products is reviewed. Recent technological advancements in suitable microorganism development, bioprocess engineering for biomass conversion for cellulosic sugars production and various fermentation strategies and downstream recovery of these top 12 products is presented. Each bio-chemical selected by US Department of Energy i.e. ethanol, xylitol/sorbitol, furans (5-HMF, 2,5-FDCA,), glycerol & its derivatives, hydrocarbons) isoprene, iso-butadienes and others), lactic acid, succinic acid, 3-hydroxy propionic acid, levulinic acid and biohydrogen/biogas is included in a single book chapter. In addition to the technical aspects of these 12 biochemicals, general technological challenges dealing with lignocellulose refining, perspectives and solutions are elaborated in the book. Also, life cycle analysis, techno-economic viability, and sustainability index of biofuels/biochemicals are comprehensively reviewed in the book. covers uniquely designed scientific and technical literature on USDOE top listed biochemicals production with clear images and tables in the context of biomass valorisation Includes the clear and simplistic illustration of technological updates on biomass processing, system biology, microbial fermentation, catalysis, regeneration and monitoring of renewable energy and chemicals production Presents fast and reliable source of information on techno-economic analysis, life cycle analysis, technological scouting at industrial scale Entails fundamental aspects, recent developments in production of renewable chemicals as building block materials for commodity chemicals production

Book Description :

Current Status and Future Scope of Microbial Cellulases not only explores the present and future of cellulase production, it also compares solid state fermentation (SSF) and submerged fermentation (SMF) for cellulase production. Chapters explore bioprocess engineering, metabolic engineering and genetic engineering approaches for enhanced cellulase production, including the application of cellulase for biofuel production. This important resource presents current technical status and the future direction of advances in cellulase production, including application of cellulases in different sectors. Covers the present industrial scenarios and future prospect of cellulase production Describes the molecular structure of cellulase Explores genetic engineering, metabolic engineering and other approaches for improved cellulase production Includes different applications of cellulases, including their application in the bioenergy sector

Book Description :

This new book, Bioethanol: Biochemistry and Biotechnological Advances, presents some insightful perspectives and important advances in the bioethanol industry. The volume goes into detail on the biochemical and physiological parameters carried out by the main bioethanol-producing microorganisms as well as the discusses the potential applications that bioproducts can have and the advantages they generate. The chapter authors discuss a variety of issues, including the physiology of ethanol production by yeasts, by Zymomonas mobilis, and by Clostridium thermocellum. Other sources of biofuel, such as sweet sorghum, Agave americana L. leaves waste, and fungi are included as well. Chapters also discuss the genetic regulation and genetic engineering of principal microorganisms and then go on to address ways to increase ethanol tolerance in industrially important ethanol fermenting organisms, methods for developing sustainable fermentable substrates, and new strategies for ethanol purification. Chapters explore the design and engineering requirements for bioreactors, bioelectrosynthesis of ethanol via bioelectrochemical systems, and more. The book will be a valuable resource for faculty and students in this area as well as for scientists, researchers, and managers in the biofuel industry in the area of biofuel production, fermentation process, environmental engineering and all other related scientific areas.

Book Description :

This book presents the select proceedings of the International Symposium entitled “Materials of the Millennium: Emerging Trends and Future Prospects” (MMETFP 2021). It discusses the synthesis, tailoring, and characterization of different materials for functional applications in various sectors which include but not limited to energy, environment, biomedical/ health care, construction, transportation etc. Topics covered in this book are synthesis and characterization of polymers, ceramics, composites, biomaterials, carbon-based nanostructures as well as materials for green environment, structural materials, modeling and simulation of materials. The book also covers the topic of emerging trends in nanostructured materials, thin films, and devices. The book is useful for students, researchers, and professionals working in the various areas of materials science and engineering.

Book Description :

The search for alternative sources of energy to offset diminishing resources of easy and cost-effective fossil fuels has become a global initiative, and fuel generated from biomass is a leading competitor in this arena. Large-scale introduction of biofuels into the energy mix could contribute to environmentally and economicaly sustainable development on a global scale. The processes and methodologies presented in this volume will offer a cutting-edge and comprehensive approach to the production of biofuels, for engineers, researchers, and students.

Book Description :

This book presents the most up-to-date technologies for the transformation of biomass into valuable fuels, chemicals, materials, and products. It provides comprehensive coverage of the characterization and fractionation of various types of biomass and details the many challenges that are currently encountered during this process. Divided into two sections, this book discusses timely topics such as the characterization of biomass feedstock, pretreatment and fractionation of biomass, and describes the process for conversion of biomass to value-added commodities. The authors bring biomass transformational strategies that are yet to be explored to the forefront, making this innovative book useful for graduate students and researchers in academia, government, and industry.

Book Description :

Learn more about foundational and advanced topics in metabolic engineering in this comprehensive resource edited by leaders in the field Metabolic Engineering: Concepts and Applications delivers a one-stop resource for readers seeking a complete description of the concepts, models, and applications of metabolic engineering. This guide offers practical insights into the metabolic engineering of major cell lines, including E. Coli, Bacillus and Yarrowia Lipolytica, and organisms, including human, animal, and plant). The distinguished editors also offer readers resources on microbiome engineering and the use of metabolic engineering in bioremediation. Written in two parts, Metabolic Engineering begins with the essential models and strategies of the field, like Flux Balance Analysis, Quantitative Flux Analysis, and Proteome Constrained Models. It also provides an overview of topics like Pathway Design, Metabolomics, and Genome Editing of Bacteria and Eukarya. The second part contains insightful descriptions of the practical applications of metabolic engineering, including specific examples that shed light on the topics within. In addition to subjects like the metabolic engineering of animals, humans, and plants, you’ll learn more about: Metabolic engineering concepts and a historical perspective on their development The different modes of analysis, including flux balance analysis and quantitative flux analysis An illuminating and complete discussion of the thermodynamics of metabolic pathways The Genome architecture of E. coli, as well as genome editing of both bacteria and eukarya An in-depth treatment of the application of metabolic engineering techniques to organisms including corynebacterial, bacillus, and pseudomonas, and more Perfect for students of biotechnology, bioengineers, and biotechnologists, Metabolic Engineering: Concepts and Applications also has a place on the bookshelves of research institutes, biotechnological institutes and industry labs, and university libraries. It's comprehensive treatment of all relevant metabolic engineering concepts, models, and applications will be of use to practicing biotechnologists and bioengineers who wish to solidify their understanding of the field.

Book Description :

Biotechnology for Biofuel Production and Optimization is the compilation of current research findings that cover the entire process of biofuels production from manipulation of genes and pathways to organisms and renewable feedstocks for efficient biofuel production as well as different cultivation techniques and process scale-up considerations. This book captures recent breakthroughs in the interdisciplinary areas of systems and synthetic biology, metabolic engineering, and bioprocess engineering for renewable, cleaner sources of energy. Describes state-of-the-art engineering of metabolic pathways for the production of a variety of fuel molecules Discusses recent advances in synthetic biology and metabolic engineering for rational design, construction, evaluation of novel pathways and cell chassis Covers genome engineering technologies to address complex biofuel-tolerant phenotypes for enhanced biofuel production in engineered chassis Presents the use of novel microorganisms and expanded substrate utilization strategies for production of targeted fuel molecules Explores biohybrid methods for harvesting bioenergy Discusses bioreactor design and optimization of scale-up

Book Description :

The global warming problem is becoming critical year by year, causing climate disaster all over the world, where it has been believed that the CO2 gas emitted from the factories and the burning of fossil fuels may be one of the reasons of global warming. Moreover, the global stock of fossil fuels is limited, and may run out soon within several tens of years. Although wind, geo-thermal, and tide energies have been considered as clean energy sources, those depend on the land or sea locations and subject to the climate change. Biofuel and biochemical production from renewable bio-resources has thus been paid recent attention from environmental protection and energy production points of view, where the current chemical and energy producing plants can be also utilized with slight modification. The so-called 1st generation biofuels have been produced from corn starch and sugarcane in particular in USA and Brazil. However, this causes the problem of the so-called "food and energy issues" as the production scale increases. The 2nd generation biofuel production from lingo-cellulosic biomass or wastes has thus been paid recent attention. However, it requires energy intensive pretreatment for the degradation of lingo-cellulosic biomass, and the fermentation is slow due to low growth rate, and thus the productivity of biofuels and bio-chemicals is low. The 3rd generation biofuel production from photosynthetic organisms such as cyanobacteria and algae has been also paid attention, because such organisms can grow with only sun light and CO2 in the air, but the cell growth rate and thus the productivity of the fuels is significantly low. The main part or core of such production processes is the fermentation by micro-organisms. In particular, it is critical to properly understand the cell metabolism followed by the efficient metabolic engineering. The book gives comprehensive explanation of the cell metabolism and the metabolic regulation mechanisms of a variety of micro-organisms. Then the efficient metabolic engineering approaches are explained to properly design the microbial cell factories for the efficient cell growth and biofuel and biochemical production.

Book Description :

This book covers the present and future of bioethanol biorefinery technologies. It discusses the efficient use of feedstock in bioethanol production, and critically reviews the environmental sustainability of bioethanol production. In addition, it describes the integrated production of bioelectricity, biopolymers, organic acids, and other biomolecules, as well as the use of process-related liquid and solid byproducts and/or wastes during bioethanol generation. Since the bioethanol industry has also led the automotive industry to explore new avenues, this book summarizes the various aspects of ethanol motorization, hybrid engine development, and biofuel electrification. For decades, clean and renewable alternatives have been sought to reduce dependence on petroleum-based fossil fuels and CO2 emissions. Bioethanol appears as one of the best solutions for the production of biofuels, bioenergy and biochemicals, along with the establishment of new biorefinery concepts and a circular bioeconomy. Therefore, the ideas and technologies presented in this book contribute to the UN Sustainable Development Goal 7: Affordable and Clean Energy. This book is a useful reference for postgraduate students and researchers interested in biorefinery and biofuel technologies, both in academia- and commercial laboratories. Early career scientists can use it to fast track into the field. Advanced scientists will find it helpful to gain a broader overview of the field beyond their area of specialization.

Book Description :

For researchers already familiar with biomass conversion technologies and for professionals in other fields, such as agriculture, food, and chemical industries, here is a comprehensive review of the emerging biorefinery industry. The book's content has been conveniently organized according to technologies (biomass feedstock and pretreatment, hydrolytic enzymes in biorefinery, and biofuels), with each chapter highlighting an important biobased industrial product. For undergraduate and graduate students, the book is a thorough introduction to biorefinery technologies.

Book Description :

This book addresses waste generation problems from various sectors, including industries, agriculture, and household. It focuses on how modern biotechnological approaches could help manage waste in an eco-friendly manner and generate precious bioenergy. It discusses the inadequate waste management systems damaging the environment and its adverse impacts on climate change-related problems. This book covers all the essential information regarding various types of waste and their management. It is a comprehensive compilation for understanding the efficient generation of bioenergy. It is a relevant reading material (resource) for anyone who wishes to study waste management as Chemist, Biologist, Biotechnologist, Industrialist, Ecologist, Microbiologist, Economist, and all disciplines related to the environment.

Book Description :

This book focuses on the different kinds of biofuels and biofuel resources. Biofuels represent a major type of renewable energy. As part of a larger bio-economy, they are closely linked to agriculture, forestry and manufacturing. Biofuels have the potential to improve regional energy access, reduce dependence on fossil fuels and contribute to climate protection. Further, this alternative form of energy could revitalize the forestry and agricultural sector and promote the increased use of renewable resources as raw materials in a range of industrial processes. Efforts are continuously being made to develop economically competitive biofuels, and microbes play important roles in the production of biofuels from various bioresources. This book elaborates on recent advances in existing microbial technologies and on sustainable approaches to improving biofuel production processes. Additionally, it examines trends in, and the limitations of, existing processes and technologies. The book offers a comprehensive overview of microbial bioresources, microbial technologies, advances in bioconversion and biorefineries, as well as microbial and metabolic engineering for efficient biofuel production. Readers will also learn about the environmental impacts and the influence of climate change on the sustainability of biofuel production. This book is intended for researchers and students whose work involves biorefinery technologies, microbiology, biotechnology, agriculture, environmental biology and related fields.

Book Description :

The global mandate for safer, cleaner and renewable energy has accelerated research on microbes that convert carbon sources to end-products serving as biofuels of the so-called first, second or third generation – e.g., bioethanol or biodiesel derived from starchy, sugar-rich or oily crops; bioethanol derived from composite lignocellulosic biomass; and biodiesels extracted from oil-producing algae and cyanobacteria, respectively. Recent advances in ‘omics’ applications are beginning to cast light on the biological mechanisms underlying biofuel production. They also unravel mechanisms important for organic solvent or high-added-value chemical production, which, along with those for fuel chemicals, are significant to the broader field of Bioenergy. The Frontiers in Microbial Physiology Research Topic that led to the current e-book publication, operated from 2013 to 2014 and welcomed articles aiming to better understand the genetic basis behind Bioenergy production. It invited genetic studies of microbes already used or carrying the potential to be used for bioethanol, biobutanol, biodiesel, and fuel gas production, as also of microbes posing as promising new catalysts for alternative bioproducts. Any research focusing on the systems biology of such microbes, gene function and regulation, genetic and/or genomic tool development, metabolic engineering, and synthetic biology leading to strain optimization, was considered highly relevant to the topic. Likewise, bioinformatic analyses and modeling pertaining to gene network prediction and function were also desirable and therefore invited in the thematic forum. Upon e-book development today, we, at the editorial, strongly believe that all articles presented herein – original research papers, reviews, perspectives and a technology report – significantly contribute to the emerging insights regarding microbial-derived energy production. Katherine M. Pappas, 2016

Book Description :

This volume scopes several aspects of non-conventional yeast research prepared by the leading specialists in the field. An introduction on taxonomy and systematics enhances the reader’s knowledge on yeasts beyond established ones such as Saccharomyces cerevisiae. Biotechnological approaches that involve fungal utilization of unusual substrates, production of biofuels and useful chemicals as citric acid, glutathione or erythritol are discussed. Further, strategies for metabolic engineering based on knowledge on regulation of gene expression as well as sensing and signaling pathways are presented. The book targets researchers and advanced students working in Microbiology, Microbial Biotechnology and Biochemistry.

Book Description :

Comprehensive Biotechnology, Third Edition unifies, in a single source, a huge amount of information in this growing field. The book covers scientific fundamentals, along with engineering considerations and applications in industry, agriculture, medicine, the environment and socio-economics, including the related government regulatory overviews. This new edition builds on the solid basis provided by previous editions, incorporating all recent advances in the field since the second edition was published in 2011. Offers researchers a one-stop shop for information on the subject of biotechnology Provides in-depth treatment of relevant topics from recognized authorities, including the contributions of a Nobel laureate Presents the perspective of researchers in different fields, such as biochemistry, agriculture, engineering, biomedicine and environmental science

Book Description :

This book aspires to be a comprehensive summary of current biofuels issues and thereby contribute to the understanding of this important topic. Readers will find themes including biofuels development efforts, their implications for the food industry, current and future biofuels crops, the successful Brazilian ethanol program, insights of the first, second, third and fourth biofuel generations, advanced biofuel production techniques, related waste treatment, emissions and environmental impacts, water consumption, produced allergens and toxins. Additionally, the biofuel policy discussion is expected to be continuing in the foreseeable future and the reading of the biofuels features dealt with in this book, are recommended for anyone interested in understanding this diverse and developing theme.

Book Description :

New and Future Developments in Microbial Biotechnology and Bioengineering: From Cellulose to Cellulase: Strategies to Improve Biofuel Production outlines new methods for the industrial production of the cellulose enzyme. The book compares the various processes for the production of biofuels, including the cost of cellulose production and availability. Biofuels are considered to be the main alternatives to fossil fuels in reducing environmental pollution and climate change. Currently, all existing biofuel production is suffering because of the high costs of production processes. As a result, cost effective practical implementation is needed to make this a viable energy alternative. Introduces new and innovative strategies for cellulase enzyme production at industrial scale Provides sustainable approaches to produce cellulase at low cost Covers all aspect and possible factors for economical, low cost, cellulase mediated biofuels production

Book Description :

Amongst concerns about climate change, energy security decline and depletion of fossil fuels, this book explores the high importance of and interests in alternative energy resources. Many studies have shown that biomass fuels are sustainable, environmentally friendly and can be the most appropriate replacement to the depleting crude oil. Additionally, they can expand green landscapes, create new job opportunities, be directly utilised in standard power systems and improve combustion performance. Biomass fuels can be limited due to production cost and competition with food. Therefore, plant and food wastes play an important role in reducing these costs and recycling dump bio-materials. Production of biofuels from non-food biomass has emerged as a sustainable option to tackle the problems associated with growing demands for energy.