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FRET and FLIM Techniques

FRET and FLIM Techniques Book
Author : Theodorus W. J. Gadella
Publisher : Elsevier
Release : 2011-07-29
ISBN : 9780080915128
Language : En, Es, Fr & De

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Book Description :

This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.

FRET and FLIM Techniques

FRET and FLIM Techniques Book
Author : Theodorus W. J. Gadella
Publisher : Elsevier Science
Release : 2009-01-05
ISBN : 9780080549583
Language : En, Es, Fr & De

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Book Description :

This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.

Microscopy Techniques

Microscopy Techniques Book
Author : Jens Rietdorf
Publisher : Springer Science & Business Media
Release : 2005-06-23
ISBN : 9783540236986
Language : En, Es, Fr & De

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Book Description :

With contributions by numerous experts

Methods in Cell Biology

Methods in Cell Biology Book
Author : Anonim
Publisher : Academic Press
Release : 2020-05-16
ISBN : 012820009X
Language : En, Es, Fr & De

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Book Description :

Methods in Cell Biology, Volume 158, the latest release in this series, highlights new advances in the field, with this release covering How to orient cells in micro-cavities for high resolution imaging of cytokinesis and lumen formation, A body-on-a-chip (BOC) system for studying gut-liver interaction, Manipulating cultured mammalian cells for mitosis research, Live-cell FLIM-FRET using a commercially available system, A comparative analysis of methods to measure kinetochore-microtubule attachment stability, A workflow for visualizing human cancer biopsies using large-format electron microscopy, Isolation of stage-specific germ cells using facs in drosophila germarium, Computational analysis of filament polymerization dynamics in cytoskeletal networks, and more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Cell Biology series Updated release includes the latest information in this area of study

Understanding Light Microscopy

Understanding Light Microscopy Book
Author : Jeremy Sanderson
Publisher : Wiley
Release : 2019-05-28
ISBN : 0470973757
Language : En, Es, Fr & De

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Book Description :

Introduces readers to the enlightening world of the modern light microscope There have been rapid advances in science and technology over the last decade, and the light microscope, together with the information that it gives about the image, has changed too. Yet the fundamental principles of setting up and using a microscope rests upon unchanging physical principles that have been understood for years. This informative, practical, full-colour guide fills the gap between specialised edited texts on detailed research topics, and introductory books, which concentrate on an optical approach to the light microscope. It also provides comprehensive coverage of confocal microscopy, which has revolutionised light microscopy over the last few decades. Written to help the reader understand, set up, and use the often very expensive and complex modern research light microscope properly, Understanding Light Microscopy keeps mathematical formulae to a minimum—containing and explaining them within boxes in the text. Chapters provide in-depth coverage of basic microscope optics and design; ergonomics; illumination; diffraction and image formation; reflected-light, polarised-light, and fluorescence microscopy; deconvolution; TIRF microscopy; FRAP & FRET; super-resolution techniques; biological and materials specimen preparation; and more. Gives a didactic introduction to the light microscope Encourages readers to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facility Features full-colour illustrations and workable practical protocols Understanding Light Microscopy is intended for any scientist who wishes to understand and use a modern light microscope. It is also ideal as supporting material for a formal taught course, or for individual students to learn the key aspects of light microscopy through their own study.

Advanced Time Correlated Single Photon Counting Applications

Advanced Time Correlated Single Photon Counting Applications Book
Author : Wolfgang Becker
Publisher : Springer
Release : 2015-04-13
ISBN : 3319149296
Language : En, Es, Fr & De

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Book Description :

This book is an attempt to bridge the gap between the instrumental principles of multi-dimensional time-correlated single photon counting (TCSPC) and typical applications of the technique. Written by an originator of the technique and by sucessful users, it covers the basic principles of the technique, its interaction with optical imaging methods and its application to a wide range of experimental tasks in life sciences and clinical research. The book is recommended for all users of time-resolved detection techniques in biology, bio-chemistry, spectroscopy of live systems, live cell microscopy, clinical imaging, spectroscopy of single molecules, and other applications that require the detection of low-level light signals at single-photon sensitivity and picosecond time resolution.

Fluorescence Microscopy

Fluorescence Microscopy Book
Author : Ulrich Kubitscheck
Publisher : John Wiley & Sons
Release : 2017-03-27
ISBN : 3527687726
Language : En, Es, Fr & De

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Book Description :

While there are many publications on the topic written by experts for experts, this text is specifically designed to allow advanced students and researchers with no background in physics to comprehend novel fluorescence microscopy techniques. This second edition features new chapters and a subsequent focus on super-resolution and single-molecule microscopy as well as an expanded introduction. Each chapter is written by a renowned expert in the field, and has been thoroughly revised to reflect the developments in recent years.

Molecular Imaging

Molecular Imaging Book
Author : Ammasi Periasamy,Richard Day
Publisher : Elsevier
Release : 2011-04-28
ISBN : 9780080536873
Language : En, Es, Fr & De

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Book Description :

The detection and measurement of the dynamic interactions of proteins within the living cell are critical to our understanding of cell physiology and pathophysiology. With FRET microscopy and spectroscopy techniques, basic and clinical scientists can make such measurements at very high spatial and temporal resolution. But sources of background information about these tools are very limited, so this book fills an important gap. It covers both the basic concepts and theory behind the various FRET microscopy and spectroscopy techniques, and the practical aspects of using the techniques and analyzing the results. The critical tricks for obtaining a good FRET image and precisely quantitating the signals from living specimens at the nanomolecular level are explained. Valuable information about the preparation of biological samples used for FRET image analysis is also provided. The methods covered include different types of microscopy systems and detectors (wide-field, confocal, multi-photon) as well as specialized techniques such as photobleaching FRET, FLIM-FRET microscopy, spectral imaging FRET, single molecule FRET, and time and image correlation spectroscopy. Starting from the basics, the chapters guide readers through the choice of probes to be used for FRET experiments and the selection of the most suitable experimental approaches to address specific biological questions. Up-to-date, consistently organized, and well-illustrated, this unique book will be welcomed by all researchers who wish to use FRET microscopy and spectroscopy techniques.

Biomedical Optical Imaging Technologies

Biomedical Optical Imaging Technologies Book
Author : Rongguang Liang
Publisher : Springer Science & Business Media
Release : 2012-09-21
ISBN : 364228390X
Language : En, Es, Fr & De

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Book Description :

This book provides an introduction to design of biomedical optical imaging technologies and their applications. The main topics include: fluorescence imaging, confocal imaging, micro-endoscope, polarization imaging, hyperspectral imaging, OCT imaging, multimodal imaging and spectroscopic systems. Each chapter is written by the world leaders of the respective fields, and will cover: principles and limitations of optical imaging technology, system design and practical implementation for one or two specific applications, including design guidelines, system configuration, optical design, component requirements and selection, system optimization and design examples, recent advances and applications in biomedical researches and clinical imaging. This book serves as a reference for students and researchers in optics and biomedical engineering.

Laboratory techniques in biochemistry and molecular biology

Laboratory techniques in biochemistry and molecular biology Book
Author : Theodorus Wilhelmus Johannes Gadella
Publisher : Unknown
Release : 2009
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Laboratory techniques in biochemistry and molecular biology book written by Theodorus Wilhelmus Johannes Gadella, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Advanced Time Correlated Single Photon Counting Techniques

Advanced Time Correlated Single Photon Counting Techniques Book
Author : Wolfgang Becker
Publisher : Springer Science & Business Media
Release : 2005-12-20
ISBN : 3540288821
Language : En, Es, Fr & De

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Book Description :

In 1984 Desmond O’Connor and David Phillips published their comprehensive book „Time-correlated Single Photon Counting“. At that time time-correlated s- gle photon counting, or TCSPC, was used primarily to record fluorescence decay functions of dye solutions in cuvettes. From the beginning, TCSPC was an am- ingly sensitive and accurate technique with excellent time-resolution. However, acquisition times were relatively slow due to the low repetition rate of the light sources and the limited speed of the electronics of the 70s and early 80s. Moreover, TCSPC was intrinsically one-dimensional, i.e. limited to the recording of the wa- form of a periodic light signal. Even with these limitations, it was a wonderful te- nique. More than 20 years have elapsed, and electronics and laser techniques have made impressive progress. The number of transistors on a single chip has approximately doubled every 18 months, resulting in a more than 1,000-fold increase in compl- ity and speed. The repetition rate and power of pulsed light sources have increased by about the same factor.

Advanced Imaging Techniques in Clinical Pathology

Advanced Imaging Techniques in Clinical Pathology Book
Author : Francesco M. Sacerdoti,Antonio Giordano,Carlo Cavaliere
Publisher : Humana Press
Release : 2016-08-23
ISBN : 1493934694
Language : En, Es, Fr & De

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Book Description :

This text provides a comprehensive, state-of-the-art review of the application of image analysis focusing on the techniques which can be used in every biology and medical laboratory to automate procedures of cell analysis and to create statistics very useful for a comprehension of cell growth dynamics and the effects of drugs on them. This textbook will serve as a very useful resource for physicians and researchers dealing with, and interested in, cell analysis. It will provide a concise yet comprehensive summary of the current status of the field that will help guide patient management and stimulate investigative efforts. All chapters are written by experts in their fields and include the most up-to-date scientific and clinical information. Advanced Imaging Techniques in Clinical Pathology will be of great value to clinical pathologists, biologists, biology researchers, and those working in the clinical and biological laboratory arena.​

Nanoscopy and Multidimensional Optical Fluorescence Microscopy

Nanoscopy and Multidimensional Optical Fluorescence Microscopy Book
Author : Alberto Diaspro
Publisher : CRC Press
Release : 2010-04-26
ISBN : 1420078895
Language : En, Es, Fr & De

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Book Description :

"Alberto Diaspro has been choreographing light’s dance for over 20 years, and in Nanoscopy and Multidimensional Optical Fluorescence Microscopy, he has assembled a diverse group of experts to explain the methods they use to coax light to reveal biology’s secrets." — From the Foreword by Daniel Evanko, editor, Nature Methods Nanoscopy and Multidimensional Optical Fluorescence Microscopy demonstrates that the boundaries between sciences do blur at the bottom, especially those that might separate the optical work of physicists and the cellular work of microbiologists. In 18 chapters written by pioneering researchers, this work offers the first comprehensive and current documentation of the cutting-edge research being accomplished in a wide range of photonic devices with revolutionary application. The highlight of the book is its coverage of optical nanoscopy and super-resolution microscopy. The rapid advances in this area over the past few years offer researchers in both photonics and molecular biologya wealth of accomplishment upon which they can build. Offering a complete treatment of this emerging field, this volume: Describes how scientists have exploited the properties of light and its fluorophore partners to overcome the resolution limit of conventional light microscopy Delves into recent ways to minimize the photobleaching that has long hampered many methods including those that have the potential to capture previously unobtainable information on the movements of single molecules Discusses the principles, benefits, and implementation of fluorescence correlation spectroscopy and related methods, which simplifies analysis by limiting light to stationary focal points in a sample Considers the most basic as well as emerging methods for improving three-dimensional optical sectioning microscopy Reviews the basics of FRET (fluorescence resonance energy transfer) and considers its new use for investigating protein complexes The text also introduces those emerging nonfluorescence microscopy methods that can actually exert mechanical forces to trap and move a variety of objects ranging from beads to living cells and cellular organelles. Combining this technique with fluorescence microscopy provides an unparalleled ability to manipulate and visualize biological samples. In the half-century since Richard Feynman challenged scientists to come up with the tools to investigate and manipulate our world at the nanoscale, we have succeeded in placing tools in the hands of biophysicists that are leading to major breakthroughs in our understanding of life and our ability to diagnose, treat, and prevent many challenges to human health. This book reflects what has been accomplished to date while pointing the way to what still needs to be done.

Methods in Cell Matrix Adhesion

Methods in Cell Matrix Adhesion Book
Author : Josephine Adams
Publisher : Academic Press
Release : 2002-06-12
ISBN : 0080524966
Language : En, Es, Fr & De

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Book Description :

Critically acclaimed for more than 25 years, the Methods in Cell Biology series provides an indispensable tool for the researcher. Each volume is carefully edited by experts to contain state-of-the-art reviews and step-by-step protocols. Techniques are described completely so that methods are made accessible to users. This volume, Methods of Cell-Matrix Adhesion, contains integrated coverage on cell-matrix adhesion methods. It brings the classical methodologies and the latest techniques together in one concise volume. This coverage includes experimental protocols and their conceptual background for all aspects of cell-matrix adhesion research: the extracellular matrix, adhesion receptors, and the growing number of functional applications of matrix-adhesion in molecular cell biology. Also covered is the purification of the extracellular matrix to functional analyses of cellular responses.

Imaging and Spectroscopic Analysis of Living Cells

Imaging and Spectroscopic Analysis of Living Cells Book
Author : Anonim
Publisher : Academic Press
Release : 2012-01-25
ISBN : 0123918693
Language : En, Es, Fr & De

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Book Description :

This volume of Methods in Enzymology is the first of three parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike. Expert authors who are leaders in the field Extensively referenced and useful figures and tables Provides hints and tricks to facilitate reproduction of methods

Reviews in Fluorescence 2005

Reviews in Fluorescence 2005 Book
Author : Chris D. Geddes,Joseph R. Lakowicz
Publisher : Springer Science & Business Media
Release : 2007-12-31
ISBN : 0387236902
Language : En, Es, Fr & De

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Book Description :

Last year we launched Volume 1 of the Reviews in Fluorescence series. The volume was well-received by the fluorescence community, with many e-mails and letters providing valuable feedback, we subsequently thank you all for your continued support. After the volume was published we were most pleased to learn that the volume is to be citable and indexed, appearing on the ISI database. Subsequently, as well as the series having an impact number in due course, individual chapters will appear on the database and be both citable and keyword searchable. We feel that this will be a powerful resource to both authors and readers, further disseminating leading-edge fluorescence based material. Our intention with this new series is to both disseminate and archive the most recent developments in both past and emerging fluorescence based disciplines. While all chapters are invited, we welcome and indeed encourage the fluorescence community to suggest areas of interest that they feel need to be covered by the series. In this new volume. Reviews in Fluorescence 2005, Volume 2, we have invited reviews in areas such as: Multi-dimensional Time-correlated Single Photon Counting; Fluorescence Correlation Spectroscopy; RNA folding; Lanthanide Probes and Fluorescent Biosensors to name but just a few. We hope you find this volume a useful resource and we look forward to receiving any suggestions you may have. Finally we would like to thank the authors for their timely articles, Caroleann Aitken for the fi-ont cover design, Kadir Asian for typesetting and Mary Rosenfeld for administrative support.

Modern Tools for Time Resolved Luminescence Biosensing and Imaging

Modern Tools for Time Resolved Luminescence Biosensing and Imaging Book
Author : Anonim
Publisher : Frontiers Media SA
Release : 2021-09-01
ISBN : 2889712524
Language : En, Es, Fr & De

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Book Description :

Download Modern Tools for Time Resolved Luminescence Biosensing and Imaging book written by , available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Fluorescence Based Biosensors

Fluorescence Based Biosensors Book
Author : Anonim
Publisher : Academic Press
Release : 2012-12-31
ISBN : 012386951X
Language : En, Es, Fr & De

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Book Description :

One of the major challenges of modern biology and medicine consists in finding means to visualize biomolecules in their natural environment with the greatest level of accuracy, so as to gain insight into their properties and behaviour in a physiological and pathological setting. This has been achieved thanks to the design of novel imaging agents, in particular to fluorescent biosensors. Fluorescence Biosensors comprise a large set of tools which are useful for fundamental purposes as well as for applications in biomedicine, drug discovery and biotechnology. These tools have been designed and engineered thanks to the combined efforts of chemists and biologists over the last decade, and developed hand in hand together with imaging technologies. This volume will convey the many exciting developments the field of fluorescent biosensors and reporters has witnessed over the recent years, from concepts to applications, including chapters on the chemistry of fluorescent probes, on technologies for monitoring protein/protein interactions and technologies for imaging biosensors in cultured cells and in vivo. Other chapters are devoted to specific examples of genetically-encoded reporters, or to protein and peptide biosensors, together with examples illustrating their application to cellular and in vivo imaging, biomedical applications, drug discovery and high throughput screening. Contributions from leading authorities Informs and updates on all the latest developments in the field

Bio nanoimaging

Bio nanoimaging Book
Author : Vladimir Uversky,Yuri Lyubchenko
Publisher : Academic Press
Release : 2013-11-05
ISBN : 0123978211
Language : En, Es, Fr & De

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Book Description :

Bio-Nanoimaging: Protein Misfolding & Aggregation provides a unique introduction to both novel and established nanoimaging techniques for visualization and characterization of misfolded and aggregated protein species. The book is divided into three sections covering: - Nanotechnology and nanoimaging technology, including cryoelectron microscopy of beta(2)-microglobulin, studying amyloidogensis by FRET; and scanning tunneling microscopy of protein deposits - Polymorphisms of protein misfolded and aggregated species, including fibrillar polymorphism, amyloid-like protofibrils, and insulin oligomers - Polymorphisms of misfolding and aggregation processes, including multiple pathways of lysozyme aggregation, misfolded intermediate of a PDZ domain, and micelle formation by human islet amyloid polypeptide Protein misfolding and aggregation is a fast-growing frontier in molecular medicine and protein chemistry. Related disorders include cataracts, arthritis, cystic fibrosis, late-onset diabetes mellitus, and numerous neurodegenerative diseases like Alzheimer's and Parkinson's. Nanoimaging technology has proved crucial in understanding protein-misfolding pathologies and in potential drug design aimed at the inhibition or reversal of protein aggregation. Using these technologies, researchers can monitor the aggregation process, visualize protein aggregates and analyze their properties. Provides practical examples of nanoimaging research from leading molecular biology, cell biology, protein chemistry, biotechnology, genetics, and pharmaceutical labs Includes over 200 color images to illustrate the power of various nanoimaging technologies Focuses on nanoimaging techniques applied to protein misfolding and aggregation in molecular medicine

FRET F rster Resonance Energy Transfer

FRET   F  rster Resonance Energy Transfer Book
Author : Igor L. Medintz,Niko Hildebrandt
Publisher : John Wiley & Sons
Release : 2013-10-17
ISBN : 3527656049
Language : En, Es, Fr & De

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Book Description :

Meeting the need for an up-to-date and detailed primer on all aspects of the topic, this ready reference reflects the incredible expansion in the application of FRET and its derivative techniques over the past decade, especially in the biological sciences. This wide diversity is equally mirrored in the range of expert contributors. The book itself is clearly subdivided into four major sections. The first provides some background, theory, and key concepts, while the second section focuses on some common FRET techniques and applications, such as in vitro sensing and diagnostics, the determination of protein, peptide and other biological structures, as well as cellular biosensing with genetically encoded fluorescent indicators. The third section looks at recent developments, beginning with the use of fluorescent proteins, followed by a review of FRET usage with semiconductor quantum dots, along with an overview of multistep FRET. The text concludes with a detailed and greatly updated series of supporting tables on FRET pairs and Förster distances, together with some outlook and perspectives on FRET. Written for both the FRET novice and for the seasoned user, this is a must-have resource for office and laboratory shelves.