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Fluorescence Microscopy

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Fluorescence Microscopy

Fluorescence Microscopy Book
Author : Ulrich Kubitscheck
Publisher : John Wiley & Sons
Release : 2017-05-08
ISBN : 3527338373
Language : En, Es, Fr & De

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Book Description :

While there are many publications on the topic written by experts for experts, this text is specifically designed to allow advanced students and researchers with no background in physics to comprehend novel fluorescence microscopy techniques. This second edition features new chapters and a subsequent focus on super-resolution and single-molecule microscopy as well as an expanded introduction. Each chapter is written by a renowned expert in the field, and has been thoroughly revised to reflect the developments in recent years.

Fluorescence Microscopy

Fluorescence Microscopy Book
Author : F. W. D. Rost
Publisher : Cambridge University Press
Release : 1992
ISBN : 9780521410885
Language : En, Es, Fr & De

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Book Description :

Fluorescence microscopy is used for studying the distribution of substances which are present in very small amounts, for example in living cells. This magnificent new work provides comprehensive cover of all aspects of fluorescence microscopy - including instrumentation, applications, and the history of the technique. The first volume deals with instrumentation and techniques for fluorescence microscopy, and includes a chapter on quantification and scanning. The second volume deals with the applications of fluorescence microscopy in many fields. It includes information on autofluorescence, and an invaluable appendix provides an alphabetical list of fluorochromes, giving information concerning chemical structure, fluorescence properties, applications and suitable filter combinations.

Fluorescence Microscopy and Fluorescent Probes

Fluorescence Microscopy and Fluorescent Probes Book
Author : J. Slavík
Publisher : Springer Science & Business Media
Release : 2013-11-11
ISBN : 1489918663
Language : En, Es, Fr & De

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Book Description :

Fluorescence microscopy images can be easily integrated into current video and computer image processing systems. People like visual observation; they like to watch a television or computer screen, and fluorescence techniques are thus becoming more and more popular. Since true in vivo experiments are simple to perform, samples can be directly seen and there is always the possibility of manipulating the samples during the experiments; it is an ideal technique for biology and medicine. Images are obtained by a classical (now called wide-field) fluorescence microscope, a confocal scanning microscope, upright or inverted, with epifluorescence or transmission. Computerized image processing may improve definition, and remove glare and scattered light signal. It also makes it possible to compute ratio images (ratio imaging both in excitation and in emission) or lifetime imaging. Image analysis programs may supply a great deal of additional data of various types, starting with calculations of the number of fluorescent objects, their shapes, brightness, etc. Fluorescence microscopy data may be complemented by classical measurement in the cuvette yr by flow cytometry.

Introduction to Confocal Fluorescence Microscopy

Introduction to Confocal Fluorescence Microscopy Book
Author : Michiel Muller
Publisher : SPIE Press
Release : 2006
ISBN : 9780819460431
Language : En, Es, Fr & De

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Book Description :

This book provides a comprehensive account of the theory of image formation in a confocal fluorescence microscope as well as a practical guideline to the operation of the instrument, its limitations, and the interpretation of confocal microscopy data. The appendices provide a quick reference to optical theory, microscopy-related formulas and definitions, and Fourier theory.

Optical Fluorescence Microscopy

Optical Fluorescence Microscopy Book
Author : Alberto Diaspro
Publisher : Springer Science & Business Media
Release : 2010-11-25
ISBN : 9783642151750
Language : En, Es, Fr & De

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Book Description :

In the last decade, fluorescence microscopy has evolved from a classical “retrospective” microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world of optical microscopy, this book covers topics such as the concept of white confocal, nonlinear optical microscopy, fluctuation spectroscopies, site-specific labeling of proteins in living cells, imaging molecular physiology using nanosensors, measuring molecular dynamics, muscle braking and stem cell differentiation.

Fluorescence Microscopy

Fluorescence Microscopy Book
Author : Ulrich Kubitscheck
Publisher : John Wiley & Sons
Release : 2013-04-02
ISBN : 3527671617
Language : En, Es, Fr & De

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Book Description :

A comprehensive introduction to advanced fluorescence microscopy methods and their applications. This is the first title on the topic designed specifically to allow students and researchers with little background in physics to understand both microscopy basics and novel light microscopy techniques. The book is written by renowned experts and pioneers in the field with a rather intuitive than formal approach. It always keeps the nonexpert reader in mind, making even unavoidable complex theoretical concepts readily accessible. All commonly used methods are covered. A companion website with additional references, examples and video material makes this a valuable teaching resource: http://www.wiley-vch.de/home/fluorescence_microscopy/

Quantitative Fluorescence Microscopy

Quantitative Fluorescence Microscopy Book
Author : F. W. D. Rost,Rost
Publisher : Cambridge University Press
Release : 1991-07-26
ISBN : 9780521394222
Language : En, Es, Fr & De

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Book Description :

Quantitative fluorescence microscopy is concerned with making measurements from fluorescent specimens in a fluorescence microscope, by measuring fluorescence emission from a defined area or areas of a specimen. This technique is most commonly used to determine the amount of some specific substance, such as DNA, in some particular area of a cell. But it has many other uses; for example, it can be used to identify certain substances in the cell by examining their fluorescence characteristics. This book is a complete guide to this technique for all biologists. It describes the principles and applications of quantitative fluorescence microscopy and also gives much practical information about the instrumentation required. There is also a discussion of the exciting developments in confocal fluorescence microscopy which allows the three dimensional distribution of particular substances to be determined. Everyone presently using this technique, or wishing to start using it will need to read this book.

Fluorescence Microscopy in Life Sciences

Fluorescence Microscopy in Life Sciences Book
Author : Juan Carlos Stockert ,Alfonso Blazquez-Castro
Publisher : Bentham Science Publishers
Release : 2017-12-15
ISBN : 1681085186
Language : En, Es, Fr & De

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Book Description :

Fluorescence Microscopy is a precise and widely employed technique in many research and clinical areas nowadays. Fluorescence Microscopy In Life Sciences introduces readers to both the fundamentals and the applications of fluorescence microscopy in the biomedical field as well as biological research. Readers will learn about physical and chemical mechanisms giving rise to the phenomenon of luminescence and fluorescence in a comprehensive way. Also, the different processes that modulate fluorescence efficiency and fluorescence features are explored and explained.

Protein Localization by Fluorescence Microscopy

Protein Localization by Fluorescence Microscopy Book
Author : Viki Allan
Publisher : OUP Oxford
Release : 1999-11-18
ISBN : 9780191565915
Language : En, Es, Fr & De

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Book Description :

There is an ever-increasing number of genes that have been sequenced but are of completely unknown function. The ability to determine the location of such gene products within the cell, either by the use of antibodies or by the production of chimeras with green fluorescent protein, is a vital step towards understanding what they do. This is one major reason why fluorescence microscopy is enjoying a revival. This no-nonsense guide provides detailed, practical advice on all aspects of the subject: from choosing the right equipment, to interpreting results. It balances the advantages of a wide range of techniques - including live cell work - against the potential pitfalls, offering invaluable "tricks of the trade" along the way. Protein Localization by Fluorescence Light Microscopy: A Practical Approach has something to offer all microscopists, giving a solid grounding to the novice whilst extending the range of the experienced user.

Fluorescence Microscopy

Fluorescence Microscopy Book
Author : B. Herman
Publisher : Garland Science
Release : 2020-08-27
ISBN : 1000141004
Language : En, Es, Fr & De

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Book Description :

This book covers the fundamental principles of fluorescence and their application to fluorescence microscopy, and presents applications to immunofluorescence, in situ hybridization, and photomicrography. It provides troubleshooting guidance to guide the user through commonly encountered problems.

Fluorescence Microscopy

Fluorescence Microscopy Book
Author : Anda Cornea,P. Michael Conn
Publisher : Elsevier
Release : 2014-02-24
ISBN : 0124167136
Language : En, Es, Fr & De

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Book Description :

Fluorescence Microscopy: Super-Resolution and other Novel Techniques delivers a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science. With contributions selected for clarity, utility, and reproducibility, the work provides practical tools for investigating these ground-breaking developments. Emphasizing super-resolution techniques, light sheet microscopy, sample preparation, new labels, and analysis techniques, this work keeps pace with the innovative technical advances that are increasingly vital to biological and biomedical researchers. With its extensive graphics, inter-method comparisons, and tricks and approaches not revealed in primary publications, Fluorescence Microscopy encourages readers to both understand these methods, and to adapt them to other systems. It also offers instruction on the best visualization to derive quantitative information about cell biological structure and function, delivering crucial guidance on best practices in related laboratory research. Presents a timely and comprehensive review of novel techniques in fluorescence imaging as applied to biological and biomedical research Offers insight into common challenges in implementing techniques, as well as effective solutions

Nanoscopy and Multidimensional Optical Fluorescence Microscopy

Nanoscopy and Multidimensional Optical Fluorescence Microscopy Book
Author : Alberto Diaspro
Publisher : CRC Press
Release : 2010-04-26
ISBN : 1420078895
Language : En, Es, Fr & De

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Book Description :

"Alberto Diaspro has been choreographing light’s dance for over 20 years, and in Nanoscopy and Multidimensional Optical Fluorescence Microscopy, he has assembled a diverse group of experts to explain the methods they use to coax light to reveal biology’s secrets." — From the Foreword by Daniel Evanko, editor, Nature Methods Nanoscopy and Multidimensional Optical Fluorescence Microscopy demonstrates that the boundaries between sciences do blur at the bottom, especially those that might separate the optical work of physicists and the cellular work of microbiologists. In 18 chapters written by pioneering researchers, this work offers the first comprehensive and current documentation of the cutting-edge research being accomplished in a wide range of photonic devices with revolutionary application. The highlight of the book is its coverage of optical nanoscopy and super-resolution microscopy. The rapid advances in this area over the past few years offer researchers in both photonics and molecular biologya wealth of accomplishment upon which they can build. Offering a complete treatment of this emerging field, this volume: Describes how scientists have exploited the properties of light and its fluorophore partners to overcome the resolution limit of conventional light microscopy Delves into recent ways to minimize the photobleaching that has long hampered many methods including those that have the potential to capture previously unobtainable information on the movements of single molecules Discusses the principles, benefits, and implementation of fluorescence correlation spectroscopy and related methods, which simplifies analysis by limiting light to stationary focal points in a sample Considers the most basic as well as emerging methods for improving three-dimensional optical sectioning microscopy Reviews the basics of FRET (fluorescence resonance energy transfer) and considers its new use for investigating protein complexes The text also introduces those emerging nonfluorescence microscopy methods that can actually exert mechanical forces to trap and move a variety of objects ranging from beads to living cells and cellular organelles. Combining this technique with fluorescence microscopy provides an unparalleled ability to manipulate and visualize biological samples. In the half-century since Richard Feynman challenged scientists to come up with the tools to investigate and manipulate our world at the nanoscale, we have succeeded in placing tools in the hands of biophysicists that are leading to major breakthroughs in our understanding of life and our ability to diagnose, treat, and prevent many challenges to human health. This book reflects what has been accomplished to date while pointing the way to what still needs to be done.

Fluorescence Microscopy with Tailored Illumination Light

Fluorescence Microscopy with Tailored Illumination Light Book
Author : Jialei Tang
Publisher :
Release : 2020
ISBN :
Language : En, Es, Fr & De

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Book Description :

Fluorescence microscopy has long been a valuable tool for biological and medical imaging. Control of optical parameters such as the amplitude, phase, polarization and propagation angle of light gives fluorescence imaging great capabilities ranging from single molecule imaging to long-term observation of living organisms. While numerous fluorescence imaging techniques have been developed over the past decades, there is always an inevitable tradeoff among the spatial resolution, imaging speed, contrast, photodamage and the total cost when it comes to choose the appropriate microscope. A main goal of my dissertation research is to develop state-of-the-art microscope systems that exhibit unprecedented performance in single-molecule fluorescence imaging and live-cell imaging for broader biomedical applications by tailoring the optical illumination beams. In details, I have designed and prototyped: 1) a highly inclined swept illumination for wide-field fluorescence microscope, which greatly improves the sectioning capability with a large field of view and ultrasensitivity; 2) dual inclined line-scan confocal microscope, which reduces photodamage while maintaining the background rejection capability compared to conventional line-scan confocal microscope; 3) a static non-diffracting light-sheet generation by controlling the spatial coherence of light emitting diodes and laser.

Fundamentals of Fluorescence Microscopy

Fundamentals of Fluorescence Microscopy Book
Author : Partha Pratim Mondal,Alberto Diaspro
Publisher : Springer Science & Business Media
Release : 2013-12-12
ISBN : 9400775458
Language : En, Es, Fr & De

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Book Description :

This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices. The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.

Fluorescence microscopy for detecting adhesives on fracture surfaces

Fluorescence microscopy for detecting adhesives on fracture surfaces Book
Author : John T. Quirk
Publisher :
Release : 1968
ISBN :
Language : En, Es, Fr & De

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Book Description :

When wood-adhesive joints fail it is sometimes difficult or almost impossible to judge whether failure is in adhesion or in cohesion. Failure in adhesion implies separation between the adhesive film and the wood substrate. Failure in cohesion implies that separation occurs within the wood specimen or within the glue film; thus it is necessary to assess the location and type of failure. It is particularly important to be able to detect the presence of very thin films of adhesive on the wood surface. Several survey methods using a compound research microscope and various staining techniques were investigated to select an applicable method for detecting these films. Fluorescence microscopy utilizing ultraviolet (UV) radiation proved to be the fastest and most efficient way of examining surfaces for location of fracture. (Author).

Ultra high Resolution Fluorescence Microscopy and Its Application in Biology

Ultra high Resolution Fluorescence Microscopy and Its Application in Biology Book
Author : Lin Shao
Publisher :
Release : 2005
ISBN :
Language : En, Es, Fr & De

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Book Description :

Several new resolution enhancement schemes for fluorescence microscopy are experimentally implemented and tested using beads and biological samples. The first scheme, 3D structured illumination microscopy (SIM), makes use of the simple fact that fluorescence emission is the multiplication of the illumination and the sample's dye structure. If the illumination is structured such as a sinusoidal pattern, the recorded image would contain high-frequency components encoded in terms of low frequencies. In so doing, normally undetectable high-resolution information is given a chance to appear in the observable region, and is thus made effectively detectable. We are able to double the resolution both laterally and axially of the conventional microscope with 3D SIM. The second scheme, called I5S, also uses structured illumination, but with a Sagnac-like interferometer setup consisting of two opposing objective lenses used for illumination and fluorescence detection. Interference in both illumination, which gives rise to more complicated structured illumination pattern than 3D SIM, and detection can be achieved. The combined interferometric effect is seven-fold enhancement in axial resolution as well as doubled lateral resolution. Finally, we introduce a more complicated I5S scheme in which both emission wavefronts, instead of just one of them, are recorded by two CCD cameras simultaneously. The complementary phase relationship between the two wavefronts can be utilized in estimating and then correcting the phase error often difficult to avoid in I5S experiments.

Principles of Fluorescence Spectroscopy

Principles of Fluorescence Spectroscopy Book
Author : Joseph R. Lakowicz
Publisher : Springer Science & Business Media
Release : 2007-12-05
ISBN : 9780387463124
Language : En, Es, Fr & De

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Book Description :

The third edition of this established classic text reference builds upon the strengths of its very popular predecessors. Organized as a broadly useful textbook Principles of Fluorescence Spectroscopy, 3rd edition maintains its emphasis on basics, while updating the examples to include recent results from the scientific literature. The third edition includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering. Includes a link to Springer Extras to download files reproducing all book artwork, for easy use in lecture slides. This is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology and medicine.