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Essential Enzyme Kinetics

Essential Enzyme Kinetics Book
Author : Daniel L. Purich
Publisher : Academic Press
Release : 2020-06-01
ISBN : 0128218096
Language : En, Es, Fr & De

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Book Description :

Essential Enzyme Kinetics: A Textbook for Molecular Life Scientists describes the theoretical basis and best-practice approaches for using initial-rate, fast reaction, and kinetic isotope effect experiments to define enzyme catalysis. Because a detailed knowledge of enzyme transition-states is the main driver for the rational design of slow, tight-binding inhibitors destined to become tomorrow's small-molecule drugs, Essential Enzyme Kinetics is the must-have reference for chemists, biochemists, and pharmacologists intent on pursuing careers in Big Pharma. Given the interdisciplinary nature of contemporary drug development, this book provides a lucid short-course that will also benefit nonspecialists seeking to understand the scope and reach of modern enzyme kinetics. Provides practical information about how to work with enzymes and design experiments to identify new inhibitors or activators Includes detailed step-by-step derivations of rate equations, showing tried-and-true ways to confirm that the equations obtained are correct Arranged for use both as a desk reference (with over 200 equations and more than 400 key references) or as a textbook (with 8-10 problems/exercises at the end of each chapter)

Fundamentals of Enzyme Kinetics

Fundamentals of Enzyme Kinetics Book
Author : Athel Cornish-Bowden
Publisher : Elsevier
Release : 2014-05-20
ISBN : 1483161196
Language : En, Es, Fr & De

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Book Description :

Fundamentals of Enzyme Kinetics details the rate of reactions catalyzed by different enzymes and the effects of varying the conditions on them. The book includes the basic principles of chemical kinetics, especially the order of a reaction and its rate constraints. The text also gives an introduction to enzyme kinetics - the idea of an enzyme-substrate complex; the Michaelis-Menten equation; the steady state treatment; and the validity of its assumption. Practical considerations, the derivation of steady-state rate equations, inhibitors and activators, and two-substrate reactions are also explained. Problems after the end of each chapter have also been added, as well as their solutions at the end of the book, to test the readers' learning. The text is highly recommended for undergraduate students in biochemistry who wish to study about enzymes or focus completely on enzymology, as most of the mathematics used in this book, which have been explained in detail to remove most barriers of understanding, is elementary.

Essentials of Enzymology

Essentials of Enzymology Book
Author : Rufus O. Okotore
Publisher : Xlibris Corporation
Release : 2015-03-13
ISBN : 1503527220
Language : En, Es, Fr & De

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Book Description :

Essentials of Enzymology provides concise information on an important area of the subject, Biochemistry. This may serve as course material for an advanced treatise in Enzymology designed for undergraduate science degree programs, especially B.Sc. (Hons ) Biochemistry and Chemistry. The book is in 12 chapters which has been divided into four distinct sections, thus (1) Basic enzyme chemistry and physiology. (2) Enzyme Kinetics, (3) Enzyme catalysis, Mechanisms and Regulation,(4)Applications of Enzymology. The Part 1 consists of four chapters that deal with the nature of enzymes- (history, properties and classifi cation), enzyme physiology; structure of enzymes, and analytical enzymology. Part 2 deals with Enzyme Kinetics which is treated in three chapters, and Part 3, made up of three chapters discuss Enzyme catalysis, mechanisms and regulation. Lastly, Part 4 consisting of two chapters deal with the applications of enzymology. Signifi cantly, the kinetics of enzyme catalyzed reactions in diverse experimental conditions, and also under various inhibition types are presented in a simple, mathematical lucid approach. The mechanisms of action for two atypical proteins-chymotrypsin and lysozyme, so also the identifi cation of active sites of enzymes by specifi c labels are discussed concisely. Lastly, the specifi c applications of enzymes in diagnostic medicine, industry, and also the new emerging area of enzyme biotechnology and enzyme bioinformatics are presented

Principles of Enzyme Kinetics

Principles of Enzyme Kinetics Book
Author : Athel Cornish-Bowden
Publisher : Elsevier
Release : 2014-05-20
ISBN : 1483164675
Language : En, Es, Fr & De

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Book Description :

Principles of Enzyme Kinetics discusses the principles of enzyme kinetics at an intermediate level. It is primarily written for first-year research students in enzyme kinetics. The book is composed of 10 chapters. Chapter 1 provides the basic principles of enzyme kinetics with a brief discussion of dimensional analysis. Subsequent chapters cover topics on the essential characteristics of steady-state kinetics, temperature dependence, methods for deriving steady-state rate equations, and control of enzyme activity. Integrated rate equations, and introductions to the study of fast reactions and the statistical aspects of enzyme kinetics are provided as well. Chemists and biochemists will find the book invaluable.

Enzyme Kinetics and Regulation

Enzyme Kinetics and Regulation Book
Author : Aaren Bennett
Publisher : Scientific e-Resources
Release : 2018-07-07
ISBN : 183947176X
Language : En, Es, Fr & De

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Book Description :

We live in the age of science-the human and numerous other living beings' genomes have been sequenced and we are beginning to understand the capacity of the metabolic machinery responsible for life on our planet. A huge number of new genes have been discovered, a significant number of these coding for enzymes of yet obscure capacity. Understanding the kinetic behavior of an enzyme provides clues to its possible physiological role. From a biotechnological perspective, knowledge of the reactant properties of an enzyme is required for the design of immobilized enzyme-based modern processes. Biotransformations are of key importance to the pharmaceutical and sustenance industries, and knowledge of the reactant properties of enzymes, essential. This book is tied in with understanding the principles of enzyme kinetics and knowing how to use mathematical models to describe the reactant capacity of an enzyme. Coverage of the material is in no way, shape or form exhaustive. There exist many books on enzyme kinetics that offer intensive, in-depth treatises of the subject. Intracellular and extracellular physiological cascades are regulated by initiation and hindrance of different enzymes involved in these pathways. Investigating and understanding the mechanism of enzyme hindrance has become the premise of development of pharmaceutical agents. Organically active regular and synthetic inhibitors have been developed and special emphasis has been placed on investigations that define their structure-work relationships in an effort to understand the inception of their natural properties. A powerful complement to the assessment of these agents is the preparation and subsequent examination of key fractional structures, deep-seated auxiliary adjustments and the corresponding unnatural enantiomers of characteristic items. We sincerely hope that this book will represent an element in the tool kit of graduate students in applied science and chemical and biochemical engineering and furthermore of undergraduate students with formal preparing in natural chemistry, biochemistry, thermodynamics and chemical reaction kinetics.

Kinetics of Enzyme Action

Kinetics of Enzyme Action Book
Author : Ross L. Stein
Publisher : John Wiley and Sons
Release : 2011-05-18
ISBN : 111808439X
Language : En, Es, Fr & De

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Book Description :

Few scientists have the knowledge to perform the studies that are necessary to discover and characterize enzyme inhibitors, despite the vested interest the pharmaceutical industry has in this field. Beginning with the most basic principles pertaining to simple, one-substrate enzyme reactions and their inhibitors, and progressing to a thorough treatment of two-substrate enzymes, Kinetics of Enzyme Action: Essential Principles for Drug Hunters provides biochemists, medicinal chemists, and pharmaceutical scientists with numerous case study examples to outline the tools and techniques necessary to perform, understand, and interpret detailed kinetic studies for drug discovery.

Contemporary Enzyme Kinetics and Mechanism

Contemporary Enzyme Kinetics and Mechanism Book
Author : N.A
Publisher : Academic Press
Release : 2009-10-24
ISBN : 9780123847447
Language : En, Es, Fr & De

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Book Description :

Kinetic studies of enzyme action provide powerful insights into the underlying mechanisms of catalysis and regulation. These approaches are equally useful in examining the action of newly discovered enzymes and therapeutic agents. Contemporary Enzyme Kinetics and Mechanism, Second Edition presents key articles from Volumes 63, 64, 87, 249, 308 and 354 of Methods in Enzymology. The chapters describe the most essential and widely applied strategies. A set of exercises and problems is included to facilitate mastery of these topics. The book will aid the reader to design, execute, and analyze kinetic experiments on enzymes. Its emphasis on enzyme inhibition will also make it attractive to pharmacologists and pharmaceutical chemists interested in rational drug design. Of the seventeen chapters presented in this new edition, ten did not previously appear in the first edition. Transient kinetic approaches to enzyme mechanisms Designing initial rate enzyme assay Deriving initial velocity and isotope exchange rate equations Plotting and statistical methods for analyzing rate data Cooperativity in enzyme function Reversible enzyme inhibitors as mechanistic probes Transition-state and multisubstrate inhibitors Affinity labeling to probe enzyme structure and function Mechanism-based enzyme inactivators Isotope exchange methods for elucidating enzymatic catalysis Kinetic isotope effects in enzyme catalysis Site-directed mutagenesis in studies of enzyme catalysis

Enzyme Kinetics and Mechanism Part A Initial Rate and Inhibitor Methods

Enzyme Kinetics and Mechanism  Part A  Initial Rate and Inhibitor Methods Book
Author : N.A
Publisher : Academic Press
Release : 1979-12-12
ISBN : 9780121819637
Language : En, Es, Fr & De

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Book Description :

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.

Enzyme Kinetics and Mechanisms Part E Energetics of Enzyme Catalysis

Enzyme Kinetics and Mechanisms  Part E  Energetics of Enzyme Catalysis Book
Author : N.A
Publisher : Academic Press
Release : 1999-09-10
ISBN : 9780121822095
Language : En, Es, Fr & De

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Book Description :

This volume supplements Volumes 63, 64, 87, and 249 of Methods in Enzymology. These volumes provide a basic source for the quantitative interpretation of enzyme rate data and the analysis of enzyme catalysis. Among the major topics covered are Engergetic Coupling in Enzymatic Reactions, Intermediates and Complexes in Catalysis, Detection and Properties of Low Barrier Hydrogen Bonds, Transition State Determination, and Inhibitors. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Essentials of Enzymology

Essentials of Enzymology Book
Author : John Herald
Publisher :
Release : 2016-06-03
ISBN : 9781682862285
Language : En, Es, Fr & De

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Book Description :

Enzymology deals with in-depth study and analysis of enzymes and is crucial for the understanding of many physiological processes. The aim of this book is to provide an understanding of the multiple aspects of enzymology through discussions on topics like metabolism, enzyme kinetics, industrial applications, etc. A number of latest researches have been included to keep the readers up-to-date with the global progress in this area of study. This book is an essential guide for both researchers and students who wish to delve deeper into the scientific study of enzymes.

Enzyme Kinetics Catalysis and Control

Enzyme Kinetics  Catalysis and Control Book
Author : Daniel L. Purich
Publisher : Elsevier
Release : 2010-06-16
ISBN : 0123809258
Language : En, Es, Fr & De

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Book Description :

Far more than a comprehensive treatise on initial-rate and fast-reaction kinetics, this one-of-a-kind desk reference places enzyme science in the fuller context of the organic, inorganic, and physical chemical processes occurring within enzyme active sites. Drawing on 2600 references, Enzyme Kinetics: Catalysis & Control develops all the kinetic tools needed to define enzyme catalysis, spanning the entire spectrum (from the basics of chemical kinetics and practical advice on rate measurement, to the very latest work on single-molecule kinetics and mechanoenzyme force generation), while also focusing on the persuasive power of kinetic isotope effects, the design of high-potency drugs, and the behavior of regulatory enzymes. Historical analysis of kinetic principles including advanced enzyme science Provides both theoretical and practical measurements tools Coverage of single molecular kinetics Examination of force generation mechanisms Discussion of organic and inorganic enzyme reactions

Kinetics of Enzyme Modifier Interactions

Kinetics of Enzyme Modifier Interactions Book
Author : Antonio Baici
Publisher : Springer
Release : 2015-06-24
ISBN : 3709114020
Language : En, Es, Fr & De

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Book Description :

The kinetic mechanisms by which enzymes interact with inhibitors and activators, collectively called modifiers, are scrutinized and ranked taxonomically into autonomous species in a way similar to that used in the biological classification of plants and animals. The systematization of the mechanisms is based on two fundamental characters: the allosteric linkage between substrate and modifier and the factor by which a modifier affects the catalytic constant of the enzyme. Combinations of the physically significant states of these two characters in an ancestor-descendant-like fashion reveal the existence of seventeen modes of interaction that cover the needs of total, partial and fine-tuning modulation of enzyme activity. These interactions comprise five linear and five hyperbolic inhibition mechanisms, five nonessential activation mechanisms and two hybrid species that manifest either hyperbolic inhibition or nonessential activation characteristics depending on substrate concentration. Five essential activation mechanisms, which are taxonomically independent of the mentioned basic species, complete the inventory of enzyme modifiers. Often masked under conventional umbrella terms or treated as anomalous cases, all seventeen basic inhibition and nonessential activation mechanisms are represented in the biochemical and pharmacological literature of this and the past century, either in the form of rapid or slow-onset reversible interactions, or as irreversible modification processes. The full potential of enzyme inhibitors and activators can only be appreciated after elucidating the details of their kinetic mechanisms of action exploring the entire range of physiologically significant reactant concentrations. This book highlights the wide spectrum of allosteric enzyme modification in physiological occurrences as well as in pharmacological and biotechnological applications that embrace simple and multiple enzyme-modifier interactions. The reader is guided in the journey through this still partly uncharted territory with the aid of mechanistically-oriented criteria aimed at showing the logical way towards the identification of a particular mechanism.

Enzyme Kinetics and Mechanisms

Enzyme Kinetics and Mechanisms Book
Author : Kenneth B. Taylor
Publisher : Springer Science & Business Media
Release : 2007-05-08
ISBN : 0306480255
Language : En, Es, Fr & De

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Book Description :

Enzyme Kinetics and Mechanisms takes the reader through the experimental techniques and the logic by which the mechanisms of enzyme-catalyzed reactions can be elucidated by the results of steady-state kinetics and related experiments. It is meant to make these investigations both satisfying and effective. In distinction to other available descriptions, the descriptions in enzyme Kinetics and Mechanisms are limited to more commonly utilized and useful models and techniques. The logic relating the chemical models to the mathematical models and the logic of relating the mathematical models to data is presented in rather concise text, figures and equations. The development of mathematical models from chemical models is done by a unique algorithm that is both simple and quick, and the same concept are utilized to develop models for the effects of a variety of reaction conditions on the initial velocity. In addition, the various relationships of data, mathematical models and the chemical models is illustrated with examples from the scientific literature.

Enzyme Kinetics and Mechanism Part C Intermediates Stereochemistry and Rate Studies

Enzyme Kinetics and Mechanism  Part C  Intermediates  Stereochemistry  and Rate Studies Book
Author : N.A
Publisher : Academic Press
Release : 1982-10-12
ISBN : 9780121819873
Language : En, Es, Fr & De

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Book Description :

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.

Enzyme Kinetics

Enzyme Kinetics Book
Author : Alejandro G. Marangoni
Publisher : John Wiley & Sons
Release : 2003-04-23
ISBN : 0471461415
Language : En, Es, Fr & De

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Book Description :

Practical Enzyme Kinetics provides a practical how-to guide for beginning students, technicians, and non-specialists for evaluating enzyme kinetics using common software packages to perform easy enzymatic analyses.

Evaluation of Enzyme Inhibitors in Drug Discovery

Evaluation of Enzyme Inhibitors in Drug Discovery Book
Author : Robert A. Copeland
Publisher : John Wiley & Sons
Release : 2013-01-31
ISBN : 111854028X
Language : En, Es, Fr & De

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Book Description :

Offers essential guidance for discovering and optimizingnovel drug therapies Using detailed examples, Evaluation of Enzyme Inhibitors inDrug Discovery equips researchers with the tools needed toapply the science of enzymology and biochemistry to the discovery,optimization, and preclinical development of drugs that work byinhibiting specific enzyme targets. Readers will applaud this bookfor its clear and practical presentations, including its expertadvice on best practices to follow and pitfalls to avoid. This Second Edition brings the book thoroughly up to datewith the latest research findings and practices. Updates exploreadditional forms of enzyme inhibition and special treatments forenzymes that act on macromolecular substrates. Readers will alsofind new discussions detailing the development and application ofthe concept of drug-target residence time. Evaluation of Enzyme Inhibitors in Drug Discovery beginsby explaining why enzymes are such important drug targets and thenexamines enzyme reaction mechanisms. The book covers: Reversible modes of inhibitor interactions with enzymes Assay considerations for compound library screening Lead optimization and structure-activity relationships forreversible inhibitors Slow binding and tight binding inhibitors Drug-target residence time Irreversible enzyme inactivators The book ends with a new chapter exploring the application ofquantitative biochemical principles to the pharmacologic evaluationof drug candidates during lead optimization and preclinicaldevelopment. The Second Edition of Evaluation of Enzyme Inhibitorsin Drug Discovery continues to offer a treatment of enzymologyapplied to drug discovery that is quantitative and mathematicallyrigorous. At the same time, the clear and simple presentationsdemystify the complex science of enzymology, making the bookaccessible to many fields— from pharmacology to medicinalchemistry to biophysics to clinical medicine.

Enzyme Kinetics

Enzyme Kinetics Book
Author : Hans Bisswanger
Publisher : John Wiley & Sons
Release : 2008-06-25
ISBN : 3527622039
Language : En, Es, Fr & De

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Book Description :

This new, expanded and updated edition of the user-friendly and comprehensive treatise on enzyme kinetics expertly balances theory and practice. This is an indispensable aid for advanced students and professionals working with enzymes, whether biochemists, biotechnologists, chemical biologists, pharmacologists or bioengineers in academia, industry and clinical research.

Enzyme Kinetics and Mechanism Part B Isotopic Probes and Complex Enzyme Systems

Enzyme Kinetics and Mechanism  Part B  Isotopic Probes and Complex Enzyme Systems Book
Author : N.A
Publisher : Academic Press
Release : 1980-04-11
ISBN : 9780121819644
Language : En, Es, Fr & De

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Book Description :

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.

Enzyme Kinetics and Mechanism Part D Developments in Enzyme Dynamics

Enzyme Kinetics and Mechanism  Part D  Developments in Enzyme Dynamics Book
Author : N.A
Publisher : Academic Press
Release : 1995-04-05
ISBN : 9780121821500
Language : En, Es, Fr & De

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Book Description :

General Description of the Volume: This volume, as do the other Enzyme Kinetics and Mechanism volumes in the Methods in Enzymology series, provides treatment of dynamic and chemical approaches for investigating enzyme catalysis and regulation, as well as designing metabolic inhibitors. It will greatly interest those involved in enzyme chemistry, metabolic control, and drug design. It should also interest those developing commercial applications for enzymes whose properties have been re-engineered using recombinant DNA technology and site-directed mutagenesis. General Description of the Series: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. Mechanisms of enzyme catalysis and inhibition Enzyme structure and function Regulatory control of enzymes Action of catalytic antibodies and ribozymes

Enzymes

Enzymes Book
Author : Malcolm Dixon,Edwin C. Webb
Publisher : Elsevier
Release : 2014-06-05
ISBN : 1483225607
Language : En, Es, Fr & De

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Book Description :

Enzymes, Second Edition provides information pertinent to the developments in the field of enzymology. This book presents the properties of enzymes as chemical catalysts or simply as chemical substances. Organized into 13 chapters, this edition begins with an overview of the range of action or specificity of enzymes. This text then discusses the special techniques employed in the isolation of enzymes and explores the considerable progress in the study of the properties and functions of enzymes. Other chapters consider the mechanism of enzyme catalysis by more direct methods, including the use of isotopes. This book discusses as well the mechanism of the biosynthesis of enzymes and the means by which their chemical structure is determined by the genetic material of the chromosomes. The final chapter deals with the essential aspects of the enzymatic system linking energy-producing processes with energy-utilizing processes. This book is a valuable resource for biochemists, physical chemists, and research workers.