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Essential Enzyme Kinetics

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Essential Enzyme Kinetics

Essential Enzyme Kinetics Book
Author : Daniel L. Purich
Publisher : Academic Press
Release : 2020-06
ISBN : 9780128218082
Language : En, Es, Fr & De

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Book Description :

Essentials of Enzyme Kinetics: A Textbook for Molecular Life Scientists discusses best-practice approaches for using site-directed mutagenesis to explore enzyme catalysis. The book presents a balance of initial-rate methods, fast reaction techniques, and kinetic isotope effect approaches, providing a must-have reference for bioscience workers and investigators who seek a better understanding of enzyme kinetics. Non-biochemists entering careers in Big Pharma will also benefit from the book's content as covered topics have become main drivers in small-molecule drug development. Offers practical information on how to work with enzymes and design experiments to identify new inhibitors or activators Provides detailed, step-by-step derivations of rate equations, showing tried-and-true ways to confirm that equations are correct Includes 10-12 problems for each chapter

Enzyme Kinetics and Regulation

Enzyme Kinetics and Regulation Book
Author : Aaren Bennett
Publisher : Scientific e-Resources
Release : 2018-07-07
ISBN : 183947176X
Language : En, Es, Fr & De

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Book Description :

We live in the age of science-the human and numerous other living beings' genomes have been sequenced and we are beginning to understand the capacity of the metabolic machinery responsible for life on our planet. A huge number of new genes have been discovered, a significant number of these coding for enzymes of yet obscure capacity. Understanding the kinetic behavior of an enzyme provides clues to its possible physiological role. From a biotechnological perspective, knowledge of the reactant properties of an enzyme is required for the design of immobilized enzyme-based modern processes. Biotransformations are of key importance to the pharmaceutical and sustenance industries, and knowledge of the reactant properties of enzymes, essential. This book is tied in with understanding the principles of enzyme kinetics and knowing how to use mathematical models to describe the reactant capacity of an enzyme. Coverage of the material is in no way, shape or form exhaustive. There exist many books on enzyme kinetics that offer intensive, in-depth treatises of the subject. Intracellular and extracellular physiological cascades are regulated by initiation and hindrance of different enzymes involved in these pathways. Investigating and understanding the mechanism of enzyme hindrance has become the premise of development of pharmaceutical agents. Organically active regular and synthetic inhibitors have been developed and special emphasis has been placed on investigations that define their structure-work relationships in an effort to understand the inception of their natural properties. A powerful complement to the assessment of these agents is the preparation and subsequent examination of key fractional structures, deep-seated auxiliary adjustments and the corresponding unnatural enantiomers of characteristic items. We sincerely hope that this book will represent an element in the tool kit of graduate students in applied science and chemical and biochemical engineering and furthermore of undergraduate students with formal preparing in natural chemistry, biochemistry, thermodynamics and chemical reaction kinetics.

Enzyme Kinetics

Enzyme Kinetics Book
Author : Paul C. Engel
Publisher : Springer
Release : 1981
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

The student of biological science in his final years as an undergraduate and his first years as a graduate is expected to gain some familiarity with current research at the fron tiers of his discipline. New research work is published in a perplexing diversity of publications and is inevitably concerned with the minutiae of the subject. The sheer number of research journals and papers also causes confusion and difficulties of assimilation. Review articles usually presuppose a background knowledge of the field and are inevitably rather restricted in scope. There is thus a need for short but authoritative introductions to those areas of modern biological research which are either not dealt with in standard introductory text books or are not dealt with in sufficient detail to enable the student to go on from them to read scholarly reviews with profit. This series of books is designed to satisfy this need. The authors have been asked to produce abrief outline of their subject assuming that their readers will have read and remembered much of a standard introductory textbook of biology.

Essentials of Enzymology

Essentials of Enzymology Book
Author : Rufus O. Okotore
Publisher : Xlibris Corporation
Release : 2015-03-13
ISBN : 1503527220
Language : En, Es, Fr & De

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Book Description :

Essentials of Enzymology provides concise information on an important area of the subject, Biochemistry. This may serve as course material for an advanced treatise in Enzymology designed for undergraduate science degree programs, especially B.Sc. (Hons ) Biochemistry and Chemistry. The book is in 12 chapters which has been divided into four distinct sections, thus (1) Basic enzyme chemistry and physiology. (2) Enzyme Kinetics, (3) Enzyme catalysis, Mechanisms and Regulation,(4)Applications of Enzymology. The Part 1 consists of four chapters that deal with the nature of enzymes- (history, properties and classifi cation), enzyme physiology; structure of enzymes, and analytical enzymology. Part 2 deals with Enzyme Kinetics which is treated in three chapters, and Part 3, made up of three chapters discuss Enzyme catalysis, mechanisms and regulation. Lastly, Part 4 consisting of two chapters deal with the applications of enzymology. Signifi cantly, the kinetics of enzyme catalyzed reactions in diverse experimental conditions, and also under various inhibition types are presented in a simple, mathematical lucid approach. The mechanisms of action for two atypical proteins-chymotrypsin and lysozyme, so also the identifi cation of active sites of enzymes by specifi c labels are discussed concisely. Lastly, the specifi c applications of enzymes in diagnostic medicine, industry, and also the new emerging area of enzyme biotechnology and enzyme bioinformatics are presented

Principles of Enzyme Kinetics

Principles of Enzyme Kinetics Book
Author : Athel Cornish-Bowden
Publisher : Elsevier
Release : 2014-05-20
ISBN : 1483164675
Language : En, Es, Fr & De

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Book Description :

Principles of Enzyme Kinetics discusses the principles of enzyme kinetics at an intermediate level. It is primarily written for first-year research students in enzyme kinetics. The book is composed of 10 chapters. Chapter 1 provides the basic principles of enzyme kinetics with a brief discussion of dimensional analysis. Subsequent chapters cover topics on the essential characteristics of steady-state kinetics, temperature dependence, methods for deriving steady-state rate equations, and control of enzyme activity. Integrated rate equations, and introductions to the study of fast reactions and the statistical aspects of enzyme kinetics are provided as well. Chemists and biochemists will find the book invaluable.

Kinetics of Enzyme Action

Kinetics of Enzyme Action Book
Author : Ross L. Stein
Publisher : John Wiley & Sons
Release : 2011-08-08
ISBN : 9781118084403
Language : En, Es, Fr & De

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Book Description :

Few scientists have the knowledge to perform the studies that are necessary to discover and characterize enzyme inhibitors, despite the vested interest the pharmaceutical industry has in this field. Beginning with the most basic principles pertaining to simple, one-substrate enzyme reactions and their inhibitors, and progressing to a thorough treatment of two-substrate enzymes, Kinetics of Enzyme Action: Essential Principles for Drug Hunters provides biochemists, medicinal chemists, and pharmaceutical scientists with numerous case study examples to outline the tools and techniques necessary to perform, understand, and interpret detailed kinetic studies for drug discovery.

Fundamentals of Enzyme Kinetics

Fundamentals of Enzyme Kinetics Book
Author : Athel Cornish-Bowden
Publisher : John Wiley & Sons
Release : 2013-02-22
ISBN : 3527665498
Language : En, Es, Fr & De

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Book Description :

Now in its fourth edition, this textbook is one of the few titles worldwide to cover enzyme kinetics in its entire scope and the only one to include its implications for bioinformatics and systems biology. Multi-enzyme complexes and cooperativity are therefore treated in more detail than in any other textbook on the market. The respected and well known author is one of the most experienced researchers into the topic and writes with outstanding style and didactic clarity. As with the previous editions, he presents here steady-state kinetics and fast reactions, supplementing each chapter with problems and solutions. For the first time, this edition features a companion website providing all figures in colour www.wiley-vch.de/home/fundenzykinet

Essentials of Enzymology

Essentials of Enzymology Book
Author : John Herald
Publisher : Unknown
Release : 2016-06-03
ISBN : 9781682862285
Language : En, Es, Fr & De

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Book Description :

Enzymology deals with in-depth study and analysis of enzymes and is crucial for the understanding of many physiological processes. The aim of this book is to provide an understanding of the multiple aspects of enzymology through discussions on topics like metabolism, enzyme kinetics, industrial applications, etc. A number of latest researches have been included to keep the readers up-to-date with the global progress in this area of study. This book is an essential guide for both researchers and students who wish to delve deeper into the scientific study of enzymes.

Kinetics of Enzyme Modifier Interactions

Kinetics of Enzyme Modifier Interactions Book
Author : Antonio Baici
Publisher : Springer
Release : 2015-06-24
ISBN : 3709114020
Language : En, Es, Fr & De

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Book Description :

The kinetic mechanisms by which enzymes interact with inhibitors and activators, collectively called modifiers, are scrutinized and ranked taxonomically into autonomous species in a way similar to that used in the biological classification of plants and animals. The systematization of the mechanisms is based on two fundamental characters: the allosteric linkage between substrate and modifier and the factor by which a modifier affects the catalytic constant of the enzyme. Combinations of the physically significant states of these two characters in an ancestor-descendant-like fashion reveal the existence of seventeen modes of interaction that cover the needs of total, partial and fine-tuning modulation of enzyme activity. These interactions comprise five linear and five hyperbolic inhibition mechanisms, five nonessential activation mechanisms and two hybrid species that manifest either hyperbolic inhibition or nonessential activation characteristics depending on substrate concentration. Five essential activation mechanisms, which are taxonomically independent of the mentioned basic species, complete the inventory of enzyme modifiers. Often masked under conventional umbrella terms or treated as anomalous cases, all seventeen basic inhibition and nonessential activation mechanisms are represented in the biochemical and pharmacological literature of this and the past century, either in the form of rapid or slow-onset reversible interactions, or as irreversible modification processes. The full potential of enzyme inhibitors and activators can only be appreciated after elucidating the details of their kinetic mechanisms of action exploring the entire range of physiologically significant reactant concentrations. This book highlights the wide spectrum of allosteric enzyme modification in physiological occurrences as well as in pharmacological and biotechnological applications that embrace simple and multiple enzyme-modifier interactions. The reader is guided in the journey through this still partly uncharted territory with the aid of mechanistically-oriented criteria aimed at showing the logical way towards the identification of a particular mechanism.

Contemporary Enzyme Kinetics and Mechanism

Contemporary Enzyme Kinetics and Mechanism Book
Author : Anonim
Publisher : Academic Press
Release : 2009-10-24
ISBN : 9780123847447
Language : En, Es, Fr & De

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Book Description :

Kinetic studies of enzyme action provide powerful insights into the underlying mechanisms of catalysis and regulation. These approaches are equally useful in examining the action of newly discovered enzymes and therapeutic agents. Contemporary Enzyme Kinetics and Mechanism, Second Edition presents key articles from Volumes 63, 64, 87, 249, 308 and 354 of Methods in Enzymology. The chapters describe the most essential and widely applied strategies. A set of exercises and problems is included to facilitate mastery of these topics. The book will aid the reader to design, execute, and analyze kinetic experiments on enzymes. Its emphasis on enzyme inhibition will also make it attractive to pharmacologists and pharmaceutical chemists interested in rational drug design. Of the seventeen chapters presented in this new edition, ten did not previously appear in the first edition. Transient kinetic approaches to enzyme mechanisms Designing initial rate enzyme assay Deriving initial velocity and isotope exchange rate equations Plotting and statistical methods for analyzing rate data Cooperativity in enzyme function Reversible enzyme inhibitors as mechanistic probes Transition-state and multisubstrate inhibitors Affinity labeling to probe enzyme structure and function Mechanism-based enzyme inactivators Isotope exchange methods for elucidating enzymatic catalysis Kinetic isotope effects in enzyme catalysis Site-directed mutagenesis in studies of enzyme catalysis

Contemporary Enzyme Kinetics and Mechanism

Contemporary Enzyme Kinetics and Mechanism Book
Author : Daniel L. Purich
Publisher : Academic Press
Release : 1996-08-20
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Kinetic studies of enzyme action provide insights into the underlying mechanisms of catalysis and regulation. This volume presents key articles from Volumes 63, 64, 87 and 249 of "Methods in Enzymology". The chapters describe the most essential and widely applied strategies.

Enzyme Kinetics and Mechanisms Part E Energetics of Enzyme Catalysis

Enzyme Kinetics and Mechanisms  Part E  Energetics of Enzyme Catalysis Book
Author : Anonim
Publisher : Academic Press
Release : 1999-09-10
ISBN : 9780121822095
Language : En, Es, Fr & De

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Book Description :

This volume supplements Volumes 63, 64, 87, and 249 of Methods in Enzymology. These volumes provide a basic source for the quantitative interpretation of enzyme rate data and the analysis of enzyme catalysis. Among the major topics covered are Engergetic Coupling in Enzymatic Reactions, Intermediates and Complexes in Catalysis, Detection and Properties of Low Barrier Hydrogen Bonds, Transition State Determination, and Inhibitors. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Enzyme Biotechnology

Enzyme Biotechnology Book
Author : G. Tripathi
Publisher : Unknown
Release : 2009-01-01
ISBN : 9788183761246
Language : En, Es, Fr & De

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Book Description :

The book provides basic and relevant information on all essential aspects of enzyme biotechnology. In-depth and up-to-date description has been given on the topics of current interests such as mechanism of enzyme catalysis and immobilization, emzyme therapy, genetic engineering of enzyme and enzyme bioelectronics. In addition, the facts on enzyme kinetics, industrial applications of enzyme in organic solvents and stabilization, anzymes in clinical diagnosis, ageing and and pollution will adequately supplement the knowledge of biotechnology today and tomorrow.

Enzyme Kinetics and Mechanisms

Enzyme Kinetics and Mechanisms Book
Author : Kenneth B. Taylor
Publisher : Springer Science & Business Media
Release : 2002-07-31
ISBN : 9781402007286
Language : En, Es, Fr & De

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Book Description :

Enzyme Kinetics and Mechanisms takes the reader through the experimental techniques and the logic by which the mechanisms of enzyme-catalyzed reactions can be elucidated by the results of steady-state kinetics and related experiments. It is meant to make these investigations both satisfying and effective. In distinction to other available descriptions, the descriptions in enzyme Kinetics and Mechanisms are limited to more commonly utilized and useful models and techniques. The logic relating the chemical models to the mathematical models and the logic of relating the mathematical models to data is presented in rather concise text, figures and equations. The development of mathematical models from chemical models is done by a unique algorithm that is both simple and quick, and the same concept are utilized to develop models for the effects of a variety of reaction conditions on the initial velocity. In addition, the various relationships of data, mathematical models and the chemical models is illustrated with examples from the scientific literature. Enzyme Kinetics and Mechanisms is intended for research workers, graduate students, post doctoral associates, and faculty in biochemistry and molecular biology who are interested in the techniques and logic by which mechanisms of enzymes-catalyzed reactions can be elucidated by investigation of steady-state kinetic experiments.

Enzyme Kinetics and Mechanisms Part E Energetics of Enzyme Catalysis

Enzyme Kinetics and Mechanisms  Part E  Energetics of Enzyme Catalysis Book
Author : Anonim
Publisher : Elsevier
Release : 1999-09-06
ISBN : 0080496660
Language : En, Es, Fr & De

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Book Description :

This volume supplements Volumes 63, 64, 87, and 249 of Methods in Enzymology. These volumes provide a basic source for the quantitative interpretation of enzyme rate data and the analysis of enzyme catalysis. Among the major topics covered are Engergetic Coupling in Enzymatic Reactions, Intermediates and Complexes in Catalysis, Detection and Properties of Low Barrier Hydrogen Bonds, Transition State Determination, and Inhibitors. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Steady state Applications in Enzyme Kinetics

Steady state Applications in Enzyme Kinetics Book
Author : Charles Walter
Publisher : Unknown
Release : 1965
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Steady state Applications in Enzyme Kinetics book written by Charles Walter, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

The Theoretical Foundations of Cancer Chemotherapy Introduced by Computer Models

The Theoretical Foundations of Cancer Chemotherapy Introduced by Computer Models Book
Author : Robert C. Jackson
Publisher : Unknown
Release : 1992
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Describes the principles of cancer chemotherapy quantitatively, using computer modelling. The book includes a computer program on disk to allow the user to model tumour biology and drug effects. This allows a trial and error approach to understanding the principles of chemotherapy.

Stochastic Chemical Kinetics

Stochastic Chemical Kinetics Book
Author : Péter Érdi,Gábor Lente
Publisher : Springer
Release : 2014-05-06
ISBN : 149390387X
Language : En, Es, Fr & De

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Book Description :

This volume reviews the theory and simulation methods of stochastic kinetics by integrating historical and recent perspectives, presents applications, mostly in the context of systems biology and also in combustion theory. In recent years, due to the development in experimental techniques, such as optical imaging, single cell analysis, and fluorescence spectroscopy, biochemical kinetic data inside single living cells have increasingly been available. The emergence of systems biology brought renaissance in the application of stochastic kinetic methods.

Enzyme Kinetics and Mechanism Part F Detection and Characterization of Enzyme Reaction Intermediates

Enzyme Kinetics and Mechanism  Part F  Detection and Characterization of Enzyme Reaction Intermediates Book
Author : Daniel L. Purich
Publisher : Elsevier
Release : 2002-11-04
ISBN : 9780080489384
Language : En, Es, Fr & De

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Book Description :

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences. Spectroscopic Detection of Reaction Intermediates Isotopic and Kenetic Detection of Reaction Intermediates Chemical Trapping and Inhibitor Methods for Detecting Reaction Intermediates