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Drosophila Cells In Culture

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Drosophila Cells in Culture

Drosophila Cells in Culture Book
Author : Guy Echalier,Norbert Perrimon,Stephanie E Mohr
Publisher : Academic Press
Release : 2017-11-30
ISBN : 0128097043
Language : En, Es, Fr & De

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Book Description :

Drosophila Cells in Culture, Second Edition, includes comprehensive coverage of cell lines, methods for creating cell lines, methods for genome engineering, and the use of cell lines for genome wide rNAi screens. This publication summarizes over thirty years of experience in the handling of in vitro cultured Drosophila cells alongside recent methods and functional screens. Early and experienced researchers studying drosophila in developmental biology, genetics, neuroscience, and across the biological and biomedical sciences will benefit from this expert knowledge. Offers full coverage of cell lines and primary cultures Provides a go-to resource for methods and studies completed with drosophila cells in culture Presents a wide spectrum of experimental techniques

Drosophila Cells in Culture

Drosophila Cells in Culture Book
Author : Guy Echalier,Norbert Perrimon,Stephanie E. Mohr
Publisher : Academic Press
Release : 2017-11-24
ISBN : 9780128094730
Language : En, Es, Fr & De

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Book Description :

Drosophila Cells in Culture, Second Edition, includes comprehensive coverage of cell lines, methods for creating cell lines, methods for genome engineering, and the use of cell lines for genome wide rNAi screens. This publication summarizes over thirty years of experience in the handling of in vitro cultured Drosophila cells alongside recent methods and functional screens. Early and experienced researchers studying drosophila in developmental biology, genetics, neuroscience, and across the biological and biomedical sciences will benefit from this expert knowledge. Offers full coverage of cell lines and primary cultures Provides a go-to resource for methods and studies completed with drosophila cells in culture Presents a wide spectrum of experimental techniques

Drosophila Cells in Culture as a Model for the Study of Ecdysteroid Action

Drosophila Cells in Culture as a Model for the Study of Ecdysteroid Action Book
Author : F. Peronnet
Publisher : Unknown
Release : 1986
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Drosophila Cells in Culture as a Model for the Study of Ecdysteroid Action book written by F. Peronnet, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Infectivity of Black Beetle Virus in Cultured Drosophila Cells

Infectivity of Black Beetle Virus in Cultured Drosophila Cells Book
Author : Bernard H. Selling
Publisher : Unknown
Release : 1986
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Infectivity of Black Beetle Virus in Cultured Drosophila Cells book written by Bernard H. Selling, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Analysis of a Transformation System for Drosophila Tissue Culture Cells

Analysis of a Transformation System for Drosophila Tissue Culture Cells Book
Author : Robert Eric Moss
Publisher : Unknown
Release : 1985
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Analysis of a Transformation System for Drosophila Tissue Culture Cells book written by Robert Eric Moss, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

New Insights into Cell Culture Technology

New Insights into Cell Culture Technology Book
Author : Sivakumar Joghi Thatha Gowder
Publisher : BoD – Books on Demand
Release : 2017-05-10
ISBN : 9535131338
Language : En, Es, Fr & De

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Book Description :

The book "New Insights into Cell Culture Technology" focuses on many advanced methods and techniques concerned with cell culture. The contributing authors have discussed various developments in cell culture methods, the application of insect cells for the efficient production of heterologous proteins, the expansion of human mesenchymal stromal cells for different clinical applications, the remote sensing of cell culture experiments and concepts for the development of cell culture bioprocess, continuous production of retroviral pseudotype vectors, and the production of oncolytic measles virus vectors for cancer therapy. This book is an original contribution of experts from different parts of the globe, and the in-depth information will be a significant resource for students, scientists, and physicians who are directly dealing with cells.["Culture" is essential for human life and also the life of a cell. - Sivakumar Gowder]

Invertebrate Cell System Applications

Invertebrate Cell System Applications Book
Author : Jun Mitsuhashi
Publisher : CRC Press
Release : 2021-09-21
ISBN : 1351090755
Language : En, Es, Fr & De

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Book Description :

A useful reference for those using or interested in cultured invertebrate cells, this two-volume text provides information about techniques and advances in invertebrate tissue culture. Cell lines for Insecta, Crustacea, Mollusca, and Nematoda are introduces along with their characterizations. Developments in insect biotechnology, including foreign protein production by insect cells infected with recombinant virus are described. Fundamental studies for introducing foreign genes into cultured insect cells is also presented. Wide information on studies -at cellular levels-on pathogens of insects, plants, and vertebrates is given.

Expression of Drosophila Tissue Polarity Genes in Tissue Culture

Expression of Drosophila Tissue Polarity Genes in Tissue Culture Book
Author : Qing Shi
Publisher : Unknown
Release : 1998
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Expression of Drosophila Tissue Polarity Genes in Tissue Culture book written by Qing Shi, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Somatic Homolog Pairing in Drosophila Cell Culture

Somatic Homolog Pairing in Drosophila Cell Culture Book
Author : Benjamin Richard Williams
Publisher : Unknown
Release : 2008
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Somatic Homolog Pairing in Drosophila Cell Culture book written by Benjamin Richard Williams, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

In Vitro Screen for Roles of Drosophila Melanogaster Tumor Suppressors in Cell Line Establishment and Differential Expression Analysis of Drosophila Melanogaster Cell Lines

In Vitro Screen for Roles of Drosophila Melanogaster Tumor Suppressors in Cell Line Establishment and Differential Expression Analysis of Drosophila Melanogaster Cell Lines Book
Author : Steven E. Justiniano
Publisher : Unknown
Release : 2008
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Abstract: Research using the fruit fly Drosophila melanogaster has enhanced our understanding of many biological processes, notably the mechanisms of heredity and the genetics of early embryonic development. These breakthroughs led to additional discoveries, including the mechanisms of body patterning, organogenesis and control of proliferation. Although great progress has been made using the whole fly as a model for development and disease, in vitro experiments have also contributed to our understanding of biological processes. Cell lines are important tools for biologists primarily because millions of homogeneous cells can be cultured under defined conditions and analyzed. This is particularly important for biochemical analysis. More recently the use of RNAi in cell lines, a mechanism for gene silencing, has allowed the systematic examination of the entire genome in vitro. These RNAi screens have resulted in the discovery of functions for uncharacterized genes. These assays depend critically on the existence of appropriate cell lines and only a few relatively uncharacterized Drosophila cell lines exist. This is in part because the mechanisms that lead to the establishment of cell lines are not defined. Currently, cell lines are established by homogenization of embryos or tissues to produce primary cultures, and subsequently subculturing these cells to produce lines. During this time, cultures presumably acquire mutations, leading to the establishment of cells that can survive indefinitely in vitro. Work done on mammalian cells have revealed that genetic modifications can alter cellular activity in vitro. Activation of telomerase, and genetic alteration of oncogenes and tumor suppressors profoundly alters the behavior of cells in culture and leads these cells to acquire characteristics that allow cells to thrive in vitro. In order to identify genetic elements with similar roles in the establishment of Drosophila cell lines, I have used two approaches. The first approach was an in vitro screen of Drosophila tumor suppressors. Tumor suppressors regulate the proliferation of cells through control of the cell cycle or apoptosis, and the loss of these genes usually results in overgrowth of tissues and the formation of tumors. Homologs of mammalian tumor suppressors have been identified in the fruit fly and the loss of function of these genes often results in increased cell proliferation and/or decreased apoptosis, and consequently the growth of tumors. Because of the importance of tumor suppressors in the regulation of cell cycle and/or control of apoptosis, and because it has been observed that loss of tumor suppressors can cause tumor formation in vivo in flies, I examined the effects of loss of various tumor suppressors in vitro. I hypothesize that the loss of tumor suppressor activity in Drosophila cells in vitro results in increased cell proliferation and may help establish immortal cell cultures. I observed a significant decrease in time to confluency in primary cultures with loss of function tumor suppressor genes. Additionally, I observed a very dramatic decrease in time to confluency in primary cultures with the Pten loss of function mutation. The results of loss of Pten on cells in vitro were examined further through the use of western blotting and differential expression analysis of microarrays on a loss of Pten cell line. Pten cell lines show increased activation of Akt and upregulation of genes involved in metabolism of carbohydrates, lipids and amino acids. The second approach I used was to identify genes involved in the establishment of cell lines, and was designed to take advantage of the wealth of information generated from microarray experiments. Comparison of the transcriptional profiles of various cell types was used to identify differentially regulated genes. We identified 52 genes with differential expression between cell lines and embryos/imaginal discs. Among these 52 genes we identified 18 genes, which are differentially expressed as part of the adaptation to the new tissue culture environment. Of the 34 remaining genes 6 genes were identified as being differentially expressed exclusively in cell lines. Additional experiments are underway to determine the significance of the differential expression of these genes and identify genes underlie the immortalization and/or transformation of cell line cells.

Search for a Diffusible Antiviral Factor in Drosophila Cell Cultures Persistently Infected by Black Beetle Virus

Search for a Diffusible Antiviral Factor in Drosophila Cell Cultures Persistently Infected by Black Beetle Virus Book
Author : Neil Alan Miller-Rhoads
Publisher : Unknown
Release : 1984
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Search for a Diffusible Antiviral Factor in Drosophila Cell Cultures Persistently Infected by Black Beetle Virus book written by Neil Alan Miller-Rhoads, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Techniques in in Vitro Invertebrate Hormones and Genes

Techniques in in Vitro Invertebrate Hormones and Genes Book
Author : F. Peronnet
Publisher : Unknown
Release : 1986
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Techniques in in Vitro Invertebrate Hormones and Genes book written by F. Peronnet, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Cell Surface Proteins of Drosophila

Cell Surface Proteins of Drosophila Book
Author : Daniel F. Woods
Publisher : Unknown
Release : 1983
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Cell Surface Proteins of Drosophila book written by Daniel F. Woods, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Advances in Cell Culture

Advances in Cell Culture Book
Author : Karl Maramorosch
Publisher : Elsevier
Release : 2014-12-01
ISBN : 1483215024
Language : En, Es, Fr & De

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Book Description :

Advances in Cell Culture, Volume 1 is a compendium of critical reviews in the field of cell culture. The chapters in the book are prepared by recognized authorities in their specialized fields. The text deals with all the aspects of cell culture, which includes the growth of individual cells or cell populations, the growth of small fragments of explanted tissue, the growth of organs, and the growth of obligate parasites in cell culture systems. This volume contains research papers that discuss vertebrate, invertebrate, and plant cell culture, as well as the cultivation of obligate parasites. The book also focuses on specific topics in cell culture such as the past and future developments in the field of genetic manipulation; evaluation of antiviral compounds in cell cultures with acute or persistent virus infections; propagation of malaria parasites in vitro; regulation of plant organogenesis; and insect cells for insect virus production. Cell biologists and researchers who use in vitro techniques will find the book highly informative and insightful.

Insect Cell Biotechnology

Insect Cell Biotechnology Book
Author : Gordon D. O. Maramorosch
Publisher : CRC Press
Release : 2018-01-18
ISBN : 135109047X
Language : En, Es, Fr & De

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Book Description :

Insect Cell Biotechnology provides a lucid, up-to-date description of recent major advances in the field. A number of significant topics are addressed, including the use and production of baculoviruses in insect cells, baculovirus specificity, bacterial toxin studies in cultured insect cells, scale-up operations required in the production of recombinant protein and insect viruses propagated in insect cells, growth and nervous system interactions, and the physiological and developmental capacities of cell lines. Transfection in Drosophila cells and a chapter on the theoretical and practical implications of stress produced by x-rays, ultraviolet light, chemicals, psoralens, and heat are discussed as well.

Heat Shock and the Cytoskeleton in Drosophila Tissue Culture Cells

Heat Shock and the Cytoskeleton in Drosophila Tissue Culture Cells Book
Author : Anonim
Publisher : Unknown
Release : 1986
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

This thesis represents a contribution to recent work on the involvement of the cytoskeleton and in particular the intermediate filaments in the early heat shock response.Following a discussion of key works in the fields of heat shock and the cytoskeleton, the thesis reports on a series of experiments designed primarily to investigate further the heat dependent association of a 46K protein (related immunologically to vertebrate vimentin) with the nuclear fraction in a Drosophila melanogaster Dl cell line.The main findings are: (1) the association of the 46K protein with the nuclear fraction appears to depend on whether the protein is present in an intermediate filamentous form, in non-heat shock cells grown as a monolayer culture, the 46K protein fractionates with the nuclear fraction and form part of the Triton X-100 high salt intermediate filament insoluble fraction. in non-heat shock cells grown in suspension the 46K protein associates with the nuclear and intermediate filament insoluble fractions only if the cells have been subjected to physical stress (including heat shock) prior to fractionation. This suggests therefore that heat shock causes the formation (rather than the disruption) of an intermediate filament network. The reported perinuclear localization of the intermediate filaments during heat shock suggests that the filaments might have a function in anchoring the nucleus in the cell during heat shock. (2) A protein of mwt 68K related immunologically to the 46K protein continues to be synthesized under heat shock conditions. This protein is distinct from hsp 70. Interestingly hsp 70 (and the small heat shock proteins) fractionate with the intermediate filament insoluble fraction and hsp 70 is also shown to cross react with a monoclonal antibody that recognizes a shared epitope on all intermediate filaments. (3) Different populations of Drosophila Dl cells, transfected cells, and different cell lines produce variable amounts of 46K protein. Culture conditions also effect the amount of protein made by the cells.

Heat Response and the Cytoskeleton in Drosophila Tissue Culture Cells

Heat Response and the Cytoskeleton in Drosophila Tissue Culture Cells Book
Author : S. Saunders,University of Sussex
Publisher : Unknown
Release : 1986
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Heat Response and the Cytoskeleton in Drosophila Tissue Culture Cells book written by S. Saunders,University of Sussex, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Studies on DNA Transfection and Cell Fusion in Drosophila Tissue Culture Cells

Studies on DNA Transfection and Cell Fusion in Drosophila Tissue Culture Cells Book
Author : Catherine J. C. Wardle
Publisher : Unknown
Release : 1991
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Studies on DNA Transfection and Cell Fusion in Drosophila Tissue Culture Cells book written by Catherine J. C. Wardle, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Primary Culture of Drosophila Melanogaster Glial Cells

Primary Culture of Drosophila Melanogaster Glial Cells Book
Author : Uzma Hussain
Publisher : Unknown
Release : 2014
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Glial cells are one of the most abundant cell types in human brains, yet there is very little that we actually know about these cells. We do know that glial cells are important and vital regulators of the function of the nervous system, and thus further research on the abilities and capabilities of glial cells will expand our breadth of knowledge about the nervous system. According to past research, the morphology and basic function of glial cells is quite similar in humans and in the fruitfly, Drosophila melanogaster. Thus, further research and investigation into the potential of glial cells in Drosophila melanogaster can potentially add to our understanding of glial cells and the nervous system (Parker et al. 2006). In this project, embryonic cells from Drosophila melanogaster are cultured in a specified medium followed a 24-hour period of egg laying. The culture is then incubated for a 24-hour period, followed immediately by immunohistochemistry. Through antibody staining using anti-Repo to detect for the presence of glial cells, it was confirmed that glial cells were present and thriving. This protocol could be applied to future research, which requires the culturing of glial cells.