Skip to main content

Drosophila Cells In Culture

Download Drosophila Cells In Culture Full eBooks in PDF, EPUB, and kindle. Drosophila Cells In Culture is one my favorite book and give us some inspiration, very enjoy to read. you could read this book anywhere anytime directly from your device.

Drosophila Cells in Culture

Drosophila Cells in Culture Book
Author : Guy Echalier,Norbert Perrimon,Stephanie E Mohr
Publisher : Academic Press
Release : 2017-11-30
ISBN : 0128097043
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

Drosophila Cells in Culture, Second Edition, includes comprehensive coverage of cell lines, methods for creating cell lines, methods for genome engineering, and the use of cell lines for genome wide rNAi screens. This publication summarizes over thirty years of experience in the handling of in vitro cultured Drosophila cells alongside recent methods and functional screens. Early and experienced researchers studying drosophila in developmental biology, genetics, neuroscience, and across the biological and biomedical sciences will benefit from this expert knowledge. Offers full coverage of cell lines and primary cultures Provides a go-to resource for methods and studies completed with drosophila cells in culture Presents a wide spectrum of experimental techniques

New Insights into Cell Culture Technology

New Insights into Cell Culture Technology Book
Author : Sivakumar Joghi Thatha Gowder
Publisher : BoD – Books on Demand
Release : 2017-05-10
ISBN : 9535131338
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

The book "New Insights into Cell Culture Technology" focuses on many advanced methods and techniques concerned with cell culture. The contributing authors have discussed various developments in cell culture methods, the application of insect cells for the efficient production of heterologous proteins, the expansion of human mesenchymal stromal cells for different clinical applications, the remote sensing of cell culture experiments and concepts for the development of cell culture bioprocess, continuous production of retroviral pseudotype vectors, and the production of oncolytic measles virus vectors for cancer therapy. This book is an original contribution of experts from different parts of the globe, and the in-depth information will be a significant resource for students, scientists, and physicians who are directly dealing with cells.["Culture" is essential for human life and also the life of a cell. - Sivakumar Gowder]

Invertebrate Cell System Applications

Invertebrate Cell System Applications Book
Author : Jun Mitsuhashi
Publisher : CRC Press
Release : 2018-01-18
ISBN : 1351090747
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

A useful reference for those using or interested in cultured invertebrate cells, this two-volume text provides information about techniques and advances in invertebrate tissue culture. Cell lines for Insecta, Crustacea, Mollusca, and Nematoda are introduces along with their characterizations. Developments in insect biotechnology, including foreign protein production by insect cells infected with recombinant virus are described. Fundamental studies for introducing foreign genes into cultured insect cells is also presented. Wide information on studies -at cellular levels-on pathogens of insects, plants, and vertebrates is given.

Drosophila melanogaster Practical Uses in Cell and Molecular Biology

Drosophila melanogaster  Practical Uses in Cell and Molecular Biology Book
Author : Anonim
Publisher : Academic Press
Release : 1995-01-25
ISBN : 9780080859422
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

Drosophila melanogaster: Practical Uses in Cell and Molecular Biology is a compendium of mostly short technical chapters designed to provide state-of-the art methods to the broad community of cell biologists, and to put molecular and cell biological studies of flies into perspective. The book makes the baroque aspects of genetic nomenclature and procedure accessible to cell biologists. It also contains a wealth of technical information for beginning or advanced Drosophila workers. Chapters, written within a year of publication, make this topical volume a valuable laboratory guide today and an excellent general reference for the future. Key Features * Collection of ready-to-use, state-of-the art methods for modern cell biological and related research using Drosophila melanogaster * Accessible to both experienced Drosophila researchers and to others who wish to join in at the cutting edge of this system * Drosophila offers an easily managed life cycle, inexpensive lifestyle, extraordinarily manipulable molecular and classical genetics, now combined with powerful new cell biology techniques * Introduction and overview sections orient the user to the Drosophila literature and lore * Six full-color plates and over 100 figures and tables enhance the understanding of these cell biology techniques

Vectors

Vectors Book
Author : Raymond L. Rodriguez,David T. Denhardt
Publisher : Butterworth-Heinemann
Release : 2014-05-20
ISBN : 1483103080
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

Vectors: A Survey of Molecular Cloning Vectors and Their Uses focuses on the functions of molecular cloning vectors. The book first discusses bacterial plasmid pBR322. Topics include criteria for plasmid vector design, construction and structure, transcriptional signals, DNA replication, recombination, mobilization, and plasmid stability. The text also examines bacteriophage lambda cloning vectors; filamentous phages as cloning vectors; chimeric single-stranded DNA phage-plasmid cloning vectors; and phage-plasmid hybrid vectors. The selection discusses cosmids and plasmid positive selection vectors, including library and construction, cosmid rescue, and positive selection vectors using plasmid-encoded lethal function. The text also examines vectors for regulating expression of cloned DNA, including lambda promoters, secretion vectors, and protein fusion vectors. The book takes a look at vectors with adjustable copy numbers. Copy number and protein production; adjustable copy number vectors; future expression vectors; rate-limiting steps of protein production; and promoters and ribosome binding sites are explained. The text puts emphasis on vectors for the synthesis of specific RNAs in vitro and cloning vectors for gram-positive bacteria. The selection is a valuable source of data for readers interested in molecular cloning vectors.

Circadian Rhythms

Circadian Rhythms Book
Author : Ezio Rosato
Publisher : Springer Science & Business Media
Release : 2008-02-04
ISBN : 1597452572
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

This is an in-depth examination of circadian biology, presented by leading researchers in the field. Methods for analysis of rhythmic readouts in select model organisms are included. This cutting-edge collection of protocols is adaptable for research at every level, and represents the huge strides that chronobiologists have made over the past two decades. Circadian biologists at all research levels will realize tremendous benefit from this extraordinary collection.

Muscle Development in Drosophilia

Muscle Development in Drosophilia Book
Author : Helen Sink
Publisher : Springer Science & Business Media
Release : 2007-02-26
ISBN : 0387329633
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

The different aspects of muscle development are considered from cellular, molecular and genetic viewpoints, and the text is supported by black/white and color illustrations. The book will appeal to those studying muscle development and muscle biology in any organism.

Invertebrate Tissue Culture

Invertebrate Tissue Culture Book
Author : Noriaki Agui
Publisher : Unknown
Release : 1976
ISBN : 0987650XXX
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

Download Invertebrate Tissue Culture book written by Noriaki Agui, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Drosophila Protocols

Drosophila Protocols Book
Author : William Sullivan,M. Ashburner,R. Scott Hawley
Publisher : CSHL Press
Release : 2000
ISBN : 9780879695866
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

This exceptional laboratory manual describes thirty-seven procedures most likely to be used in the next decade for molecular, biochemical, and cellular studies on Drosophila. They were selected after extensive consultation with the research community and rigorously edited for clarity, uniformity, and conciseness.The methods included permit investigation of chromosomes, cell biology, molecular biology, genomes, biochemistry, and development. Each protocol includes the basic information needed by novices, with sufficient detail to be valuable to experienced investigators. Each method is carefully introduced and illustrated with figures, tables, illustrations, and examples of the data obtainable. The book's appendices include key aspects of Drosophila biology, essential solutions, buffers, and recipes.An evolution of Michael Ashburner's 1989 classic Drosophila: A Laboratory Manual, this book is an essential addition to the personal library of Drosophila investigators and an incomparable resource for other research groups with goals likely to require fly-based technical approaches.

Invertebrate Tissue Culture

Invertebrate Tissue Culture Book
Author : Edouard Kurstak
Publisher : Elsevier
Release : 2012-12-02
ISBN : 0323151469
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

Invertebrate Tissue Culture: Applications in Medicine, Biology, and Agriculture comprises the proceedings of the IV International Conference on Invertebrate Tissue Culture, held on June 5-8, 1975 at Mont Gabriel, Quebec, Canada. The conference focuses on invertebrate organ, tissue, and cell culture, as well as cell culture limitations, pitfalls, and applications in medicine, biology, agriculture, neurophysiology, and studies of morphogenesis, differentiation, viruses, symbionts, and parasites. This reference material specifically provides information on sophisticated laboratory methods and on numerous utilizations of invertebrate cell culture techniques in medicine and biology. This book also elucidates the nutritional requirements and the establishment of cell lines. The study of viruses and protozoa of agricultural and forest importance is also shown. This book will be useful and stimulating to the readers and will provide in a single volume the results obtained in the diverse areas of research pursued by the leading exponents of invertebrate tissue culture from America, Europe, Asia, and Australia.

Molecular Biology of the Cell

Molecular Biology of the Cell Book
Author : Bruce Alberts
Publisher : Unknown
Release : 2004
ISBN : 9780815332183
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

Download Molecular Biology of the Cell book written by Bruce Alberts, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Drosophila Methods and Protocols

Drosophila  Methods and Protocols Book
Author : Christian Dahmann
Publisher : Methods in Molecular Biology
Release : 2018-10-26
ISBN : 9781493981793
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

This second edition volume expands on the previous edition by presenting updated protocols for several of the techniques described in the first edition of Drosophila: Methods and Protocols and current methods that cover recent breakthroughs in Drosophila research. The book begins with a description of FlyBase--a database of genes and genomes--followed by the presentation of systems for versatile gene expression in the fly. The first few chapters in this book detail gene knockdown and editing, including CRISPR-Cas9 and protein knockdown. The next few chapters are devoted to methods describing live imaging of different tissues and organs, followed by chapters on how to quantify image data and how to probe tissue mechanics by laser ablation. The next two chapters provide methods for analyzing transcription followed by protocols to study growth, metabolism, ageing, and behavior in Drosophila. This volume concludes with chapters on electrophysiological recordings and methods to establish cell lines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Drosophila: Methods and Protocols, Second Edition is a valuable source of hands-on protocols and reviews for molecular, cell, and developmental biologists using Drosophila as model systems in their work.

Biology of Drosophila

Biology of Drosophila Book
Author : Milislav Demerec
Publisher : CSHL Press
Release : 1994
ISBN : 9780879694418
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

Biology of Drosophila was first published by John Wiley and Sons in 1950. Until its appearance, no central, synthesized source of biological data on Drosophila melanogaster was available, despite the fly's importance to science for three decades. Ten years in the making, it was an immediate success and remained in print for two decades. However, original copies are now very hard to find. This facsimile edition makes available to the fly community once again its most enduring work of reference.

Repair of Double Strand DNA Breaks in Drosophila

Repair of Double Strand DNA Breaks in Drosophila Book
Author : Wendy Susan Dezzani
Publisher : Unknown
Release : 1981
ISBN : 0987650XXX
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

Download Repair of Double Strand DNA Breaks in Drosophila book written by Wendy Susan Dezzani, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Drosophila melanogaster

Drosophila melanogaster Book
Author : Farzana Khan Perveen
Publisher : BoD – Books on Demand
Release : 2018-02-28
ISBN : 9535138537
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

This book contains 12 chapters divided into two sections. Section 1 is "Drosophila - Model for Genetics." It covers introduction, chromosomal polymorphism, polytene chromosomes, chromosomal inversion, chromosomal evolution, cell cycle regulators in meiosis and nongenetic transgenerational inheritance in Drosophila. It also includes ecological genetics, wild-type strains, morphometric analysis, cytostatics, frequencies of early and late embryonic lethals (EEL and LEL) and mosaic imaginal discs of Drosophila for genetic analysis in biomedical research. Section 2 is "Drosophila - Model for Therapeutics." It explains Drosophila as model for human diseases, neurodegeneration, heart-kidney metabolic disorders, cancer, pathophysiology of Parkinson's disease, dopamine, neuroprotective therapeutics, mitochondrial dysfunction and translational research. It also covers Drosophila role in ubiquitin-carboxyl-terminal hydrolase-L1 (UCH-L1) protein, eye development, anti-dUCH antibody, neuropathy target esterase (NTE), organophosphorous compound-induced delayed neuropathy (OPIDN) and hereditary spastic paraplegia (HSP). It also includes substrate specificities, kinetic parameters of recombinant glutathione S-transferases E6 and E7 (DmGSTE6 and DmGSTE7), detoxification and insecticidal resistance and antiviral immunity in Drosophila.

Insect Cell Culture Engineering

Insect Cell Culture Engineering Book
Author : Mattheus F. A. Goosen
Publisher : CRC Press
Release : 2020-07-24
ISBN : 1000105660
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

Consolidating and expanding current, fundamental notions of virology and animal cell cultivation, this practical reference examines the development of insect cell culture techniques for the production of recombinant proteins and insect pathogenic viruses.;Resolving on-the-job problems such as sparging cell damage and reduced infectivity cells, Insect Cell Culture Engineering: includes special introductory material as well as background information on insect pathogenic viruses, the molecular biology of baculoviruses and bioreactor design; offers advice on how to save time when deciding which insect cell line, bioreactor and medium to exploit; discusses the preparation of mathematical modelling in animal cell culture; addresses the concerns associated with insect cell immobilization and the use of serum-free culture media; provides insights into the protective effects of polymer additives and insect cell gene expression in pharmaceutical research; and analyzes process scale-up and reactor design.;Bridging the gap between laboratory research and pilot plant scale insect culture/baculovirus technology, Insect Cell Culture Engineering is designed as a reference for biochemical and bioprocess engineers, bioprocess technologists, biochemists, molecular and cell biologists, microbiologists, and upper-level undergraduate and graduate students in these disciplines.

Drosophila Genetics

Drosophila Genetics Book
Author : Ulrich Graf,Nancy van Schaik,Friedrich E. Würgler
Publisher : Springer Science & Business Media
Release : 2012-12-06
ISBN : 3642768059
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

The Biological Sciences are in the midst of a scientific rev olution. During the past decade under the rubric of molecu lar biology, chemistry and physics have assumed an integral role in biological research. This is especially true in ge netics, where the cloning of genes and the manipulation of genomic DNA have become in many organisms routine laboratory procedures. These noteworthy advances, it must be empha sized, especially in molecular genetics, are not autonomous. Rather, they have been accomplished with those organisms whose formal genetics has been documented in great detail. For the beginning student or the established investigator who is interested in pursuing eukaryote molecular genetic re search, Drosophila melanogaster, with its rich body of formal genetic information is one organism of choice. The book "Drosophila Genetics. A Practical Course" is an indispens able source of information for the beginner in the biology and formal genetics of Drosophila melanogaster. The scope of this guide, a revision and enlargement of the original German language version, is broad and instructive. The information included ranges from the simple, but necessary, details on how to culture and manipulate Drosophila flies to a series of more sophisticated genetic experiments. After completing the experiments detailed in the text, all students - neophyte or experienced - will be richly rewarded by having acquired a broad base of classical genetics information relevant for the biologist in its own right and prerequisite to Drosophila genetics research - formal and/or molecular. Davis, California, Melvin M.

Epithelial Cell Culture Protocols

Epithelial Cell Culture Protocols Book
Author : Scott H. Randell,M. Leslie Fulcher
Publisher : Humana Press
Release : 2016-08-23
ISBN : 9781493956791
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

Fascinating biology occurs at epithelial interfaces, whether between organism and environment or within body compartments, and many diseases inflicting huge personal and societal burdens result from dysfunction of epithelial systems, e.g., carcinomas. Epithelial cell cultures have been an integral and crucial part of the biomedical research enterprise, adding unique capabilities and enabling mechanistic approaches. In the past decade there have been many research advances, such as directed differentiation of embryonic stem cells and induced pluripotent stem cells, robotic high throughput screening, whole genome siRNA and shRNA libraries, massively parallel sequencing at low cost, identification of somatic stem cells in key organs, to name a few. Epithelial Cell Culture Protocols, Second Edition provides a cross section of up-to-date culture protocols for the most heavily studied cell systems and featured supporting technologies. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Epithelial Cell Culture Protocols, Second Edition will serve outstanding investigators with the best possible information for the advancement of biomedical science.

Cytoskeleton Methods and Protocols

Cytoskeleton Methods and Protocols Book
Author : Ray H. Gavin
Publisher : Unknown
Release : 2016
ISBN : 9781493931255
Language : En, Es, Fr & De

DOWNLOAD

Book Description :

The third edition of this volume focuses on experimental models that are useful for investigating various aspects of cytoskeleton structure and function. Animal, plant, protist, and fungal models highlight twenty-four chapters that provide detailed protocols for live and fixed-cell imaging, dynamics of cytoskeleton components, cell and organelle motility, and genetics and proteomics. Written in the highly successful Methods in Molecular Biology series format, protocols in each chapter are up-to-date menus organized in a useful step-by-step format appropriate for novice and established investigators. Each chapter is equipped with a valuable notes section that provides a troubleshooting guide and helpful, and often unpublished, technical information aimed at ensuring success with implementation of the protocols. Authoritative and thorough, Cytoskeleton Methods and Protocols, Third Edition helps researchers expand their understanding of cytoskeleton structure and function.