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Drosophila Cells In Culture

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Drosophila Cells in Culture

Drosophila Cells in Culture Book
Author : Guy Echalier,Norbert Perrimon,Stephanie E Mohr
Publisher : Academic Press
Release : 2017-11-30
ISBN : 0128097043
Language : En, Es, Fr & De

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Book Description :

Drosophila Cells in Culture, Second Edition, includes comprehensive coverage of cell lines, methods for creating cell lines, methods for genome engineering, and the use of cell lines for genome wide rNAi screens. This publication summarizes over thirty years of experience in the handling of in vitro cultured Drosophila cells alongside recent methods and functional screens. Early and experienced researchers studying drosophila in developmental biology, genetics, neuroscience, and across the biological and biomedical sciences will benefit from this expert knowledge. Offers full coverage of cell lines and primary cultures Provides a go-to resource for methods and studies completed with drosophila cells in culture Presents a wide spectrum of experimental techniques

The Culture in Vitro of Cells from Lethal Embryos of Drosophila Melanogaster

The Culture in Vitro of Cells from Lethal Embryos of Drosophila Melanogaster Book
Author : N.A
Publisher :
Release : 1975
ISBN :
Language : En, Es, Fr & De

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Book Description :

The merits of utilising the Drosophila melanogaster embryo for the genetic analysis of cellular differentiation are discussed. The advantages are seen to lie in the ease of isolation and manipulation of lethal mutations, and the possibility that mutants of specific developmental processes can be selected. A major disadvantage of the Drosophila embryo is considered to be the problem of discovering whether a lethal mutant directly affects a particular cell type or not; in the whole embryo, the specific effects of a lethal factor may often be confused with their developmental ramifications. It is proposed that in vitro culture might provide a straightforward method of assessing the effects of a lethal mutation on individual cell types.In vitro cultures were made from dispersed cells of gastrula-stage lethal embryos. In the early part of the work a number of temperature-sensitive embryonic lethals were isolated and one of them was cultured by a technique utilising bulk quantities of embryos. The major conclusion from this work was that especially careful attention would have to be paid to genetic background when culturing lethal mutants in vitro. Following this, a technique for the production of high quality cultures from single embryos was developed. Whereas a previously- reported technique had been claimed to give the differentiation of nerve and rnusclo cells, the new technique gavexiiithe differentiation of five identified larval cell types, and, furthermore, the cultueres could continue to develop for several weeks. This new technique of culturing sing le embryos was applied to a number of embryonic lethals, both unconditional and temperature-sensitive.The results of culturing experiments were consist ent with, and extended, those from earlier studies of the lethal mutants which had utilised other techniques (examination of live embryos and stained sections; in vivo culture). Whilst the results show that in vitro culture limits the possibility of deleterious interaction between different cell types, and therefore renders the identific ation of specific genetic effects more straightforward, they provide strong circumstantial evidence that some interactions do take place in vitro. The consequences of such interactions are discussed, and means for the assessment of their importance in the development of cultures of mutant cells are suggested. It is pointed out that it may be possible to use in vitro culture systems to experimentally analyse mutants of known cellular interactions.

Drosophila melanogaster Practical Uses in Cell and Molecular Biology

Drosophila melanogaster  Practical Uses in Cell and Molecular Biology Book
Author : N.A
Publisher : Academic Press
Release : 1995-01-25
ISBN : 9780080859422
Language : En, Es, Fr & De

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Book Description :

Drosophila melanogaster: Practical Uses in Cell and Molecular Biology is a compendium of mostly short technical chapters designed to provide state-of-the art methods to the broad community of cell biologists, and to put molecular and cell biological studies of flies into perspective. The book makes the baroque aspects of genetic nomenclature and procedure accessible to cell biologists. It also contains a wealth of technical information for beginning or advanced Drosophila workers. Chapters, written within a year of publication, make this topical volume a valuable laboratory guide today and an excellent general reference for the future. Key Features * Collection of ready-to-use, state-of-the art methods for modern cell biological and related research using Drosophila melanogaster * Accessible to both experienced Drosophila researchers and to others who wish to join in at the cutting edge of this system * Drosophila offers an easily managed life cycle, inexpensive lifestyle, extraordinarily manipulable molecular and classical genetics, now combined with powerful new cell biology techniques * Introduction and overview sections orient the user to the Drosophila literature and lore * Six full-color plates and over 100 figures and tables enhance the understanding of these cell biology techniques

Heat Shock and the Cytoskeleton in Drosophila Tissue Culture Cells

Heat Shock and the Cytoskeleton in Drosophila Tissue Culture Cells Book
Author : N.A
Publisher :
Release : 1986
ISBN :
Language : En, Es, Fr & De

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Book Description :

This thesis represents a contribution to recent work on the involvement of the cytoskeleton and in particular the intermediate filaments in the early heat shock response.Following a discussion of key works in the fields of heat shock and the cytoskeleton, the thesis reports on a series of experiments designed primarily to investigate further the heat dependent association of a 46K protein (related immunologically to vertebrate vimentin) with the nuclear fraction in a Drosophila melanogaster Dl cell line.The main findings are: (1) the association of the 46K protein with the nuclear fraction appears to depend on whether the protein is present in an intermediate filamentous form, in non-heat shock cells grown as a monolayer culture, the 46K protein fractionates with the nuclear fraction and form part of the Triton X-100 high salt intermediate filament insoluble fraction. in non-heat shock cells grown in suspension the 46K protein associates with the nuclear and intermediate filament insoluble fractions only if the cells have been subjected to physical stress (including heat shock) prior to fractionation. This suggests therefore that heat shock causes the formation (rather than the disruption) of an intermediate filament network. The reported perinuclear localization of the intermediate filaments during heat shock suggests that the filaments might have a function in anchoring the nucleus in the cell during heat shock. (2) A protein of mwt 68K related immunologically to the 46K protein continues to be synthesized under heat shock conditions. This protein is distinct from hsp 70. Interestingly hsp 70 (and the small heat shock proteins) fractionate with the intermediate filament insoluble fraction and hsp 70 is also shown to cross react with a monoclonal antibody that recognizes a shared epitope on all intermediate filaments. (3) Different populations of Drosophila Dl cells, transfected cells, and different cell lines produce variable amounts of 46K protein. Culture conditions also effect the amount of protein made by the cells.

Drosophila Cytogenetics Protocols

Drosophila Cytogenetics Protocols Book
Author : Daryl S. Henderson
Publisher : Springer Science & Business Media
Release : 2004
ISBN : 1592596657
Language : En, Es, Fr & De

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Book Description :

In this book leading drosophilists describe, in step-by-step detail, all the essential techniques for studying Drosophila chromosomes and suggest new avenues for scientific exploration. It provides a comprehensive cytogenetics laboratory manual for investigatiors, one suitable not only for novices, but also highly informative for seasoned investigators.

Advances in Cell Culture

Advances in Cell Culture Book
Author : Karl Maramorosch
Publisher : Elsevier
Release : 2014-12-01
ISBN : 1483215024
Language : En, Es, Fr & De

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Book Description :

Advances in Cell Culture, Volume 1 is a compendium of critical reviews in the field of cell culture. The chapters in the book are prepared by recognized authorities in their specialized fields. The text deals with all the aspects of cell culture, which includes the growth of individual cells or cell populations, the growth of small fragments of explanted tissue, the growth of organs, and the growth of obligate parasites in cell culture systems. This volume contains research papers that discuss vertebrate, invertebrate, and plant cell culture, as well as the cultivation of obligate parasites. The book also focuses on specific topics in cell culture such as the past and future developments in the field of genetic manipulation; evaluation of antiviral compounds in cell cultures with acute or persistent virus infections; propagation of malaria parasites in vitro; regulation of plant organogenesis; and insect cells for insect virus production. Cell biologists and researchers who use in vitro techniques will find the book highly informative and insightful.

Cell Biology

Cell Biology Book
Author : Julio E. Celis,Nigel Carter,Kai Simons,J. Victor Small,Tony Hunter,David Shotton
Publisher : Elsevier
Release : 2005-11-16
ISBN : 0080454240
Language : En, Es, Fr & De

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Book Description :

This four-volume laboratory manual contains comprehensive state-of-the-art protocols essential for research in the life sciences. Techniques are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls. The important steps and results are beautifully illustrated for further ease of use. This collection enables researchers at all stages of their careers to embark on basic biological problems using a variety of technologies and model systems. This thoroughly updated third edition contains 165 new articles in classical as well as rapidly emerging technologies. Topics covered include: Cell and Tissue Culture: Associated Techniques, Viruses, Antibodies, Immunocytochemistry (Volume 1) Organelle and Cellular Structures, Assays (Volume 2) Imaging Techniques, Electron Microscopy, Scanning Probe and Scanning Electron Microscopy, Microdissection, Tissue Arrays, Cytogenetics and In Situ Hybridization, Genomics and Transgenic Knockouts and Knock-down Methods (Volume 3) Transfer of Macromolecules, Expression Systems, Gene Expression Profiling (Volume 4) Indispensable bench companion for every life science laboratory Provides the latest information on the plethora of technologies needed to tackle complex biological problems Includes numerous illustrations, some in full color, supporting steps and results

Arthropod Cell Culture Systems

Arthropod Cell Culture Systems Book
Author : Karl Maramorosch,Arthur H. McIntosh
Publisher : CRC Press
Release : 1994-06-03
ISBN : 9780849376429
Language : En, Es, Fr & De

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Book Description :

Invertebrate cell culture is increasingly being used in various areas of biological research. Research in cellular biology and pathology that previously depended primarily on in vitro investigations of vertebrate animal cell systems is now being conducted using invertebrate cells. Specialists and pioneers from the United States, Japan, Switzerland, Slovakia, and China have presented original contributions to create a well-balanced cross-section of current developments. Topics discussed include the preparation of cell culture media; cultivation of mosquito, lepidopteran, grasshopper, and tick cells; the application of such cells to mammalian and plant virus research; and diverse applications in medicine, biology, and agriculture. A special chapter devoted to the work of Japanese cell culture pioneers is also featured. All chapters are supported by tables, photographs, and up-to-date bibliographies.

Invertebrate Cell Culture Applications

Invertebrate Cell Culture Applications Book
Author : Karl Maramorosch
Publisher : Elsevier
Release : 2012-12-02
ISBN : 0323158838
Language : En, Es, Fr & De

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Book Description :

Invertebrate Cell Culture Applications assesses the status of invertebrate cell culture at a time when this method can be used to solve problems in a number of diverse disciplines. Organized into seven chapters, this book begins by discussing the development and amino acid requirements of insect cell culture. It then describes the Drosophila tissue culture and chromosomal phenomena in cell lines of this organism. This book also explains the culture conditions regulating the infection of cells by an intracellular microorganism, as well as the replication of arboviruses in arthropod in vitro systems. Lastly, the characteristics, growth requirements, and applications of tick cell culture to parasitology are explored. This book will contribute in solving biomedical and agricultural problems. This reference material will be of special interest to parasitologists, virologists, microbiologists, entomologists, geneticists, medical researchers, and graduate students in related fields of biomedical research.

Drosophila Protocols

Drosophila Protocols Book
Author : William Sullivan,M. Ashburner,R. Scott Hawley
Publisher : CSHL Press
Release : 2000
ISBN : 9780879695866
Language : En, Es, Fr & De

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Book Description :

This exceptional laboratory manual describes thirty-seven procedures most likely to be used in the next decade for molecular, biochemical, and cellular studies on Drosophila. They were selected after extensive consultation with the research community and rigorously edited for clarity, uniformity, and conciseness.The methods included permit investigation of chromosomes, cell biology, molecular biology, genomes, biochemistry, and development. Each protocol includes the basic information needed by novices, with sufficient detail to be valuable to experienced investigators. Each method is carefully introduced and illustrated with figures, tables, illustrations, and examples of the data obtainable. The book's appendices include key aspects of Drosophila biology, essential solutions, buffers, and recipes.An evolution of Michael Ashburner's 1989 classic Drosophila: A Laboratory Manual, this book is an essential addition to the personal library of Drosophila investigators and an incomparable resource for other research groups with goals likely to require fly-based technical approaches.

Laminins

Laminins Book
Author : Peter Ekblom,R. Timpl
Publisher : CRC Press
Release : 1996-09-01
ISBN : 9783718658077
Language : En, Es, Fr & De

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Book Description :

The Insect Viruses

The Insect Viruses Book
Author : Professor Departments of Entomology and Genetics Lois K Miller,Lois K. Miller,Laurence Andrew Ball
Publisher : Springer Science & Business Media
Release : 1998
ISBN : 9780306458811
Language : En, Es, Fr & De

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Book Description :

This book focuses on those virus families that are found primarily or exclusively in insects, covering all major families of insect-selective viruses except for the baculoviruses which were described in a previous volume of The Viruses series. Topics include: the large DNA viruses; the small DNA densoviruses; the RNA viruses; and the arbovirus expression systems and their potential employment in the future. Ninety-eight illustrations supplement the text.

Molecular Embryology

Molecular Embryology Book
Author : Michael J. Barry
Publisher : CRC Press
Release : 2002-04-02
ISBN : 9781560329367
Language : En, Es, Fr & De

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Book Description :

Molecular Embryology explains in simple terms the molecular interactions that transform an egg to a complex embryo that in the end gives rise to a fully-formed animal. In doing so, the book covers one hundred and fifty years of experiments that have led to our present understanding of these molecular interactions. As the text progresses, the reader will gain a sense of the developmental similarities and differences between organisms. Students studying developmental biology and embryology will find this book an extremely useful introduction to the subject and will also appeal to anyone with an interest in the most recent advances in this largely undiscovered territory.

RNA Interference Editing and Modification

RNA Interference  Editing  and Modification Book
Author : Jonatha M. Gott
Publisher : Springer Science & Business Media
Release : 2004
ISBN : 1592597750
Language : En, Es, Fr & De

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Book Description :

This volume presents a comprehensive collection of cuttingedge methods for elucidating the function of new genes and altering gene expression. These readily reproducible techniques can be used either in transient and stable gene splicing applied to worms, flies, trypanosomes, mammals, and plants, or in studying RNA editing mechanisms in a wide range of organisms, including systems that involve the conversion of one base to another and insertion/deletion editing. Topics of interest include stable and transient RNA interference, gene silencing, RNA editing, bioinformatics, small noncoding RNAs, and RNomics. Special attention is given to methods for the identification and characterization of small RNAs involved in RNA interference or modification. Readily reproducible protocols for discovering new genes or altering gene expression.

Transposable Elements

Transposable Elements Book
Author : Esra Galun
Publisher : Springer Science & Business Media
Release : 2003-07-31
ISBN : 9781402014581
Language : En, Es, Fr & De

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Book Description :

OY (])PONEOYLI TOIA YTA IIOAAOI OKOEOJII Many fail to grasp what they have seen, and cannot judge what they have learned, ErKYPEOYLI OYL1E MA®ONTEE ITINOEKOYIT although they tell themselves they know. EQYTOJII L1E L10KEOYLI Heraclitus of Ephesus, 500 BC " ... everyone that is not speckled and C~T~v: N,;~, 'T,ji~ ,~~~N ,tuN '= spotted among the goats and brown among the sheep, that shall be counted ~~N N,n =,~, c~w=== c,m stolen with me." Genesis Chapter 30 From Heraclitus of Ephesus and later philosophers, we can deduce that observation of natural phenomena, even when keen and accurate, will not result in meaningful knowledge unless combined with analysis of the mind; just as analysis of the mind without acquaintance with natural phenomena will not suffice to grasp the perceivable world. Only familiarity with phenomena combined with mental analysis will lead to additional knowledge. The citation from Genesis, Chapter 30, is part of an unusual story. It tells how Jacob received, as payment for his service to Laban, the bulk of Laban's herds. By agreement, Jacob was to receive "only" the newborn speckled and spotted goats and the newborn brown sheep that differed completely from their parents. Did Jacob know that there was instability (transposable elements?) in the pigmentation of Laban's herd? It is reasonable to assume that Jacob combined his keen observation with analysis of his mind in order to predict the outcome: most of the newborns were indeed speckled, spotted or brown.