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CRISPR and RNAi Systems

CRISPR and RNAi Systems Book
Author : Kamel A. Abd-Elsalam,Ki-Taek Lim
Publisher : Elsevier
Release : 2021-02-27
ISBN : 0128219114
Language : En, Es, Fr & De

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Book Description :

Plants are vulnerable to pathogens including fungi, bacteria, and viruses, which cause critical problems and deficits. Crop protection by plant breeding delivers a promising solution with no obvious effect on human health or the local ecosystem. Crop improvement has been the most powerful approach for producing unique crop cultivars since domestication occurred, making possible the main innovations in feeding the globe and community development. Genome editing is one of the genetic devices that can be implemented, and disease resistance is frequently cited as the most encouraging application of CRISPR/Cas9 technology in agriculture. Nanobiotechnology has harnessed the power of genome editing to develop agricultural crops. Nanosized DNA or RNA nanotechnology approaches could contribute to raising the stability and performance of CRISPR guide RNAs. This book brings together the latest research in these areas. CRISPR and RNAi Systems: Nanobiotechnology Approaches to Plant Breeding and Protection presents a complete understanding of the RNAi and CRISPR/Cas9 techniques for controlling mycotoxins, fighting plant nematodes, and detecting plant pathogens. CRISPR/Cas genome editing enables efficient targeted modification in most crops, thus promising to accelerate crop improvement. CRISPR/Cas9 can be used for management of plant insects, and various plant pathogens. The book is an important reference source for both plant scientists and environmental scientists who want to understand how nano biotechnologically based approaches are being used to create more efficient plant protection and plant breeding systems. Shows how nanotechnology is being used as the basis for new solutions for more efficient plant breeding and plant protection Outlines the major techniques and applications of both CRISPR and RNAi technologies Assesses the major challenges of escalating these technologies on a mass scale

RNA Interference and CRISPR Technologies

RNA Interference and CRISPR Technologies Book
Author : Mouldy Sioud
Publisher : Unknown
Release : 2020
ISBN : 9781071602904
Language : En, Es, Fr & De

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Book Description :

This volume explores the uses of RNAi and CRISPR interferences as a general method for inhibiting gene expression, with focus on their biological functions, design, chemical modifications, delivery, and preclinical/clinical applications. In addition to relevant backgrounds, the chapters in this book discuss simple and accurate protocols dealing with the latest advances in RNAi and CRISPR applications and look at how these methods differ from one another. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, RNA Interference and CRISPR Technologies: Technical Advances and New Therapeutic Opportunities is a valuable resource for any scientist, teachers, graduate student, postdoc, and clinician interested in this field. This book also benefits anyone in research and development in biotech and pharmaceutical companies who want to understand more about these technologies, and their applications in biology and medicine. .

Modulating Gene Expression

Modulating Gene Expression Book
Author : Aditi Singh,Mohammad W. Khan
Publisher : BoD – Books on Demand
Release : 2019-05-29
ISBN : 1838806962
Language : En, Es, Fr & De

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Book Description :

RNA interference (RNAi) is a widely used technology for gene silencing and has become a key tool in a myriad of research and lead discoveries. In recent years, the mechanism of RNAi agents has been well investigated, and the technique has been optimized for better effectiveness and safety. On the other hand, the clustered regularly interspaced short palindromic repeats (CRISPR)-associated Cas9/gRNA system is a recent, novel, targeted genome-editing technique derived from the bacterial immune system. Recent advances in gene-editing research and technologies have enabled the CRISPR Cas9 system to become a popular tool for sequence-specific gene editing to correct and modify eukaryotic systems. In this book, we will focus on the mechanisms, applications, regulations (their pros and cons), and various ways in which RNAi-based methods and CRIPSR-Cas9 technology have stimulated the modulation of gene expression, thereby making them a promising therapeutic tool to treat and prevent complex diseases and disorders.

CRISPR Cas Systems

CRISPR Cas Systems Book
Author : Rodolphe Barrangou,John van der Oost
Publisher : Springer Science & Business Media
Release : 2012-12-13
ISBN : 364234657X
Language : En, Es, Fr & De

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Book Description :

CRISPR/Cas is a recently described defense system that protects bacteria and archaea against invasion by mobile genetic elements such as viruses and plasmids. A wide spectrum of distinct CRISPR/Cas systems has been identified in at least half of the available prokaryotic genomes. On-going structural and functional analyses have resulted in a far greater insight into the functions and possible applications of these systems, although many secrets remain to be discovered. In this book, experts summarize the state of the art in this exciting field.

Epigenetics in Health and Disease

Epigenetics in Health and Disease Book
Author : Igor Kovalchuk,Olga Kovalchuk
Publisher : FT Press
Release : 2012-05-22
ISBN : 0132599511
Language : En, Es, Fr & De

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Book Description :

This is the first comprehensive, authoritative, and easy-to-understand introduction to modern epigenetics. Authored by two active researchers in the field, it introduces key concepts one step at a time, enabling students at all levels to benefit from it. The authors begin by presenting a historical overview that places epigenetics in context, and makes it clear that the field is not (as some presume) completely new. Next, they introduce and explain key epigenetic mechanisms, and discuss the roles these mechanisms may play in inheritance, organism development, health and disease, behavior, evolution, ecology, and the interaction of individual organisms with their environments. Coverage includes: non-coding RNAs in each kingdom; allelic interactions; CRYSPR; gene silencing; epigenetics of germline and epigenetic memory; epigenetic regulation of genome stability and plant stress response; and much more. The authors conclude by offering significant new insights into how knowledge of epigenetics and epigenomics may promote the development of technologies and solutions in areas ranging from behavioral neuroscience to cancer treatment, toxicology to the development of hardier crops.

The CRISPR Cas Tool Kit for Genome Editing

The CRISPR Cas Tool Kit for Genome Editing Book
Author : Aftab Ahmad
Publisher : Springer Nature
Release : 2022-10-04
ISBN : 981166305X
Language : En, Es, Fr & De

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Book Description :

Download The CRISPR Cas Tool Kit for Genome Editing book written by Aftab Ahmad, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

The Date Palm Genome Vol 2

The Date Palm Genome  Vol  2 Book
Author : Jameel M. Al-Khayri,S. Mohan Jain,Dennis V. Johnson
Publisher : Springer Nature
Release : 2021-06-12
ISBN : 3030737500
Language : En, Es, Fr & De

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Book Description :

This book is the first comprehensive assemblage of contemporary knowledge relevant to genomics and other omics in date palm. Volume 2 consists of 11 chapters. Part I, Nutritional and Pharmaceuticals Properties, covers the utilization of date palm as an ingredient of various food products, a source of bioactive compounds and the production of nanomaterials. Part II, Omics Technologies, addresses omics resources, proteomics and metabolomics. Part III, Molecular Breeding and Genome Modification, focuses on genetic improvement technologies based on mutagenesis, quantitative traits loci and genome editing. Part IV, Genomics of Abiotic and Biotic Stress, covers metagenomics of beneficial microbes to enhance tolerance to abiotic stress and the various genomics advances as they apply to insect control. This volume represents the efforts of 34 international scientists from 12 countries and contains 65 figures and 19 tables to illustrate presented concepts. Volume 1 is published under the title: Phylogeny, Biodiversity and Mapping.

Plant Abiotic Stress Physiology

Plant Abiotic Stress Physiology Book
Author : Khalid Rehman Hakeem,Tariq Aftab
Publisher : CRC Press
Release : 2022-02-17
ISBN : 1000400980
Language : En, Es, Fr & De

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Book Description :

This two-volume set highlights the various innovative and emerging techniques and molecular applications that are currently being used in plant abiotic stress physiology. Volume 1: Responses and Adaptations focuses on the responses and adaptations of plants to stress factors at the cellular and molecular levels and offers a variety of advanced management strategies and technologies. Volume 2: Molecular Advancements introduces a range of state-of-the-art molecular advances for the mitigation of abiotic stress in plants. With contributions from specialists in the field, Volume 1 first discusses the physiology and defense mechanisms of plants and the various kinds of stress, such as from challenging environments, climate change, and nutritional deficiencies. It goes on to discuss trailblazing management techniques that include genetics approaches for improving abiotic stress tolerance in crop plants along with CRISPR/CAS-mediated genome editing technologies. Volume 2 discusses how plants have developed diverse physiological and molecular adjustments to safeguard themselves under challenging conditions and how emerging new technologies can utilize these plant adaptations to enhance plant resistance. These include using plant-environment interactions to develop crop species that are resilient to climate change, applying genomics and phenomics approaches from the study of abiotic stress tolerance and more. Agriculture today faces countless challenges to meet the rising need for sustainable food supplies and guarantees of high-quality nourishment for a quickly increasing population. To ensure sufficient food production, it is necessary to address the difficult environmental circumstances that are causing cellular oxidative stress in plants due to abiotic factors, which play a defining role in shaping yield of crop plants. These two volumes help to meet these challenges by providing a rich source of information on plant abiotic stress physiology and effective management techniques.

RNA Interference

RNA Interference Book
Author : Ibrokhim Y. Abdurakhmonov
Publisher : BoD – Books on Demand
Release : 2016-04-06
ISBN : 953512272X
Language : En, Es, Fr & De

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Book Description :

RNA interference (RNAi), a hallmark of all biological sciences of twenty-first century, is an evolutionarily conserved and double-stranded RNA-dependent eukaryotic cell defense process. Opportunity to utilize an organisms own gene and to systematically induce and trigger RNAi for any desired sequence made RNAi an efficient approach for functional genomics, providing a solution for conventional longstanding obstacles in life sciences. RNAi research and application have significantly advanced during past two decades. This book RNA interference provides an updated knowledge and progress on RNAi in various organisms, explaining basic principles, types, and property of inducers, structural modifications, delivery systems/methodologies, and various successful bench-to-field or clinic applications and disease therapies with some aspects of limitations, alternative tools, safety, and risk assessment.

Asian Citrus Psyllid

Asian Citrus Psyllid Book
Author : Jawwad A. Qureshi,Philip A. Stansly
Publisher : CABI
Release : 2020-06-11
ISBN : 1786394081
Language : En, Es, Fr & De

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Book Description :

This book, written by a team of experts on the Asian citrus psyllid, gathers together everything currently known about the biology and ecology of this important pest species, examines the transmission and acquisition processes of the pathogen, and looks at current management practices and their effectiveness. The potential for new, innovative management techniques are also described, along with the economic implications of managing this rapidly establishing disease.

The Science of Stem Cells

The Science of Stem Cells Book
Author : Jonathan M. W. Slack
Publisher : John Wiley & Sons
Release : 2018-01-16
ISBN : 1119235154
Language : En, Es, Fr & De

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Book Description :

Introduces all of the essential cell biology and developmental biology background for the study of stem cells This book gives you all the important information you need to become a stem cell scientist. It covers the characterization of cells, genetic techniques for modifying cells and organisms, tissue culture technology, transplantation immunology, properties of pluripotent and tissue specific stem cells and, in particular, the relevant aspects of mammalian developmental biology. It dispels many misconceptions about stem cells—especially that they can be miracle cells that can cure all ills. The book puts emphasis on stem cell behavior in its biological context and on how to study it. Throughout, the approach is simple, direct, and logical, and evidence is given to support conclusions. Stem cell biology has huge potential for advancing therapies for many distressing and recalcitrant diseases, and its potential will be realized most quickly when as many people as possible have a good grounding in the science of stem cells. Content focused on the basic science underpinning stem cell biology Covers techniques of studying cell properties and cell lineage in vivo and in vitro Explains the basics of embryonic development and cell differentiation, as well as the essential cell biology processes of signaling, gene expression, and cell division Includes instructor resources such as further reading and figures for downloading Offers an online supplement summarizing current clinical applications of stem cells Written by a prominent leader in the field, The Science of Stem Cells is an ideal course book for advanced undergraduates or graduate students studying stem cell biology, regenerative medicine, tissue engineering, and other topics of science and biology.

Genome Engineering via CRISPR Cas9 System

Genome Engineering via CRISPR Cas9 System Book
Author : Vijai Singh,Pawan K. Dhar
Publisher : Academic Press
Release : 2020-02-18
ISBN : 0128181419
Language : En, Es, Fr & De

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Book Description :

Genome Engineering via CRISPR-Cas9 Systems presents a compilation of chapters from eminent scientists from across the globe who have established expertise in working with CRISPR-Cas9 systems. Currently, targeted genome engineering is a key technology for basic science, biomedical and industrial applications due to the relative simplicity to which they can be designed, used and applied. However, it is not easy to find relevant information gathered in a single source. The book contains a wide range of applications of CRISPR in research of bacteria, virus, algae, plant and mammalian and also discusses the modeling of drosophila, zebra fish and protozoan, among others. Other topics covered include diagnosis, sensor and therapeutic applications, as well as ethical and regulatory issues. This book is a valuable source not only for beginners in genome engineering, but also researchers, clinicians, stakeholders, policy makers, and practitioners interested in the potential of CRISPR-Cas9 in several fields. Provides basic understanding and a clear picture on how to design, use and implement the CRISPR-Cas9 system in different organisms Explains how to create an animal model for disease research and screening purposes using CRISPR Discusses the application of CRISPR-Cas9 systems in basic sciences, biomedicine, virology, bacteriology, molecular biology, neurology, cancer, industry, and many more

A Complete Guide to Gene Cloning

A Complete Guide to Gene Cloning Book
Author : Nayana Patil,Aruna Sivaram
Publisher : Springer Nature
Release : 2022
ISBN : 3030968510
Language : En, Es, Fr & De

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Book Description :

This comprehensive guide to gene cloning provides beginning and advanced readers with the background, standard techniques, practical applications, and ethical and safety considerations in the field. A one-stop reference for students, researchers in academia and industry, and anyone interested in a thorough but accessible overview.

Biotechnologies of Crop Improvement Volume 2

Biotechnologies of Crop Improvement  Volume 2 Book
Author : Satbir Singh Gosal,Shabir Hussain Wani
Publisher : Springer
Release : 2018-07-09
ISBN : 331990650X
Language : En, Es, Fr & De

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Book Description :

During the past 15 years, cellular and molecular approaches have emerged as valuable adjuncts to supplement and complement conventional breeding methods for a wide variety of crop plants. Biotechnology increasingly plays a role in the creation, conservation, characterization and utilization of genetic variability for germplasm enhancement. For instance, anther/microspore culture, somaclonal variation, embryo culture and somatic hybridization are being exploited for obtaining incremental improvement in the existing cultivars. In addition, genes that confer insect- and disease-resistance, abiotic stress tolerance, herbicide tolerance and quality traits have been isolated and re-introduced into otherwise sensitive or susceptible species by a variety of transgenic techniques. Together these transformative methodologies grant access to a greater repertoire of genetic diversity as the gene(s) may come from viruses, bacteria, fungi, insects, animals, human beings, unrelated plants or even be artificially derived. Remarkable achievements have been made in the production, characterization, field evaluation and commercialization of transgenic crop varieties worldwide. Likewise, significant advances have been made towards increasing crop yields, improving nutritional quality, enabling crops to be raised under adverse conditions and developing resistance to pests and diseases for sustaining global food and nutritional security. The overarching purpose of this 3-volume work is to summarize the history of crop improvement from a technological perspective but to do so with a forward outlook on further advancement and adaptability to a changing world. Our carefully chosen “case studies of important plant crops” intend to serve a diverse spectrum of audience looking for the right tools to tackle complicated local and global issues.

Diversity and Evolution of Butterfly Wing Patterns

Diversity and Evolution of Butterfly Wing Patterns Book
Author : Toshio Sekimura,H. Frederik Nijhout
Publisher : Springer
Release : 2017-08-29
ISBN : 9811049564
Language : En, Es, Fr & De

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Book Description :

This book facilitates an integrative understanding of the development, genetics and evolution of butterfly wing patterns. To develop a deep and realistic understanding of the diversity and evolution of butterfly wing patterns, it is essential and necessary to approach the problem from various kinds of key research fields such as “evo-devo,” “eco-devo,” ”developmental genetics,” “ecology and adaptation,” “food plants,” and “theoretical modeling.” The past decade-and-a-half has seen a veritable revolution in our understanding of the development, genetics and evolution of butterfly wing patterns. In addition, studies of how environmental and climatic factors affect the expression of color patterns has led to increasingly deeper understanding of the pervasiveness and underlying mechanisms of phenotypic plasticity. In recognition of the great progress in research on the biology, an international meeting titled “Integrative Approach to Understanding the Diversity of Butterfly Wing Patterns (IABP-2016)” was held at Chubu University, Japan in August 2016. This book consists of selected contributions from the meeting. Authors include main active researchers of new findings of corresponding genes as well as world leaders in both experimental and theoretical approaches to wing color patterns. The book provides excellent case studies for graduate and undergraduate classes in evolution, genetics/genomics, developmental biology, ecology, biochemistry, and also theoretical biology, opening the door to a new era in the integrative approach to the analysis of biological problems. This book is open access under a CC BY 4.0 license.

Systems and Synthetic Biotechnology for Production of Nutraceuticals

Systems and Synthetic Biotechnology for Production of Nutraceuticals Book
Author : Long Liu,Jian Chen
Publisher : Springer Nature
Release : 2020-01-03
ISBN : 9811504466
Language : En, Es, Fr & De

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Book Description :

This book discusses systems and synthetic biotechnologies for the production of nutraceuticals, and summarizes recent advances in nutraceutical research in terms of the physiological effects on health, potential applications, drawbacks of traditional production processes, characteristics of production strains, and advances in microbial production based on systems and synthetic biotechnology. It also examines future directions in the microbial production of nutraceuticals using systems and synthetic biology. The book is intended for researchers and graduate students in the field of molecular biology and industrial biotechnology as well as staff working in the nutraceutical industry.

RNAi Technology

RNAi Technology Book
Author : R. K. Gaur,Yedidya Gafni,P. Sharma,V. K. Gupta
Publisher : CRC Press
Release : 2016-04-19
ISBN : 1439873259
Language : En, Es, Fr & De

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Book Description :

RNAi technology is used for large-scale screens that systematically shut down each gene in the cell, which can help identify the components necessary for a particular cellular process or an event such as cell division. Exploitation of the pathway is also a promising tool in biotechnology and medicine. Introducing new technology in the study of RNA

Bacteriophages

Bacteriophages Book
Author : Malgorzata Lobocka,Waclaw T. Szybalski
Publisher : Academic Press
Release : 2012
ISBN : 0123946212
Language : En, Es, Fr & De

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Book Description :

This volume, the first of a two-part series, covers topics including historical, ecological and evolutionary considerations, genomics and molecular biology, and interaction of phages with their hosts. Contributions from leading authorities Informs and updates on all the latest developments in the field

Prokaryotic Diversity

Prokaryotic Diversity Book
Author : N. A. Logan,H. M. Lappin-Scott,P. C. F Oyston
Publisher : Cambridge University Press
Release : 2006-04-20
ISBN : 1139452797
Language : En, Es, Fr & De

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Book Description :

The true extent of prokaryote diversity, encompassing the spectrum of variability among bacteria, remains unknown. Current research efforts focus on understanding why prokaryote diversification occurs, its underlying mechanisms, and its likely impact. The dynamic nature of the prokaryotic world, and continuing advances in the technological tools available make this an important area and hence this book will appeal to a wide variety of microbiologists. Its coverage ranges from studies of prokaryotes in specialized environmental niches to broad examinations of prokaryote evolution and diversity, and the mechanisms underlying them. Topics include: bacteria of the gastrointestinal tract, unculturable organisms in the mouth and in the soil, organisms from extreme environments, the diversity of archaea and their phages, comparative genomics and the emergence of pathogens, the spread of genomic islands between clinical and environmental organisms, minimal genomes needed for life, horizontal gene transfer, phenotypic innovation, and patterns and extent of biodiversity.

Reprogramming the Genome Applications of CRISPR Cas in non mammalian systems part A

Reprogramming the Genome  Applications of CRISPR Cas in non mammalian systems part A Book
Author : Anonim
Publisher : Academic Press
Release : 2021-03-28
ISBN : 0323853226
Language : En, Es, Fr & De

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Book Description :

Reprogramming the Genome: Applications of CRISPR-Cas in Non-mammalian Systems, Part A presents a collation of chapters written by global, eminent scientists. CRISPR-Cas9 system is an RNA-mediated immune system of bacteria and archaea that protects from bacteriophage infections. It is one of the revolutionized technologies to uplift biology to the next stages. Chapters in this release include An Introduction and applications of CRISPR-Cas Systems, History, evolution and classification of CRISPR-Cas associated systems, CRISPR based bacterial genome editing and removal of pathogens, CRISPR based genome editing and removal of human viruses, CRISPR based development of RNA editing and diagnostic platform, and much more. Additional sections cover Genome engineering in insects for control of vector borne diseases, Development of insect cell line using CRISPR technology, CRISPRing protozoan parasites to better understand the biology of diseases, CRISPR based genome editing of Caenorhabditis elegans, and a variety of other important topics. Offers a basic understanding and clear picture of genome editing CRISPR-Cas systems in different organisms Explains how to create an animal model for disease diagnosis/research and reprogram CRISPR for removal of virus, bacteria, fungi, protozoan, and many more Discusses the advances, patents, applications, challenges and opportunities in CRISPR-Cas9 systems in basic sciences, biomedicine, virology, bacteriology, molecular biology, and many more