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Centrifugal Separations In Biotechnology

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Centrifugal Separations in Biotechnology

Centrifugal Separations in Biotechnology Book
Author : Wallace Woon-Fong Leung
Publisher : Butterworth-Heinemann
Release : 2020-03-13
ISBN : 0081026358
Language : En, Es, Fr & De

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Book Description :

Centrifugal Separations in Biotechnology, Second Edition, is the only book on the market devoted to centrifugal separation in biotechnology. Key topics covered include a full introduction to centrifugation, sedimentation and separation; detailed coverage of centrifuge types, including batch and semi-batch centrifuges, disk-stack and tubular decanter centrifuges; methods for increasing solids concentration; laboratory and pilot testing of centrifuges; selection and sizing centrifuges; scale-up of equipment, performance prediction and analysis of test results using numerical simulation. Centrifugal Separations in Biotechnology, Second Edition, provides guidance on troubleshooting and optimizing centrifuges, and then goes on to explore the commercial applications of centrifuges in biotechnology. It gives detailed process information and data to assist in the development of particular processes from existing systems. It is of value to professionals in the chemical, bioprocess, and biotech sectors, and all those concerned with bioseparation, bioprocessing, unit-operations and process engineering. Provides a comprehensive guide to centrifuges, their optimal development, and their operation in the biotechnology industry Updated throughout based on developments in industrial applications and advances in our understanding of centrifugal separations in biotechnology Discusses applications for the separation of proteins, DNA, mitochondria, ribosomes, lysosomes and other cellular elements Includes new sections on use of optimal polymer dosage in waste treatment, new centrifuge designs for applications in algae processing, biopharma, and more

Solid Liquid Separation

Solid Liquid Separation Book
Author : Ladislav Svarovsky
Publisher : Butterworth-Heinemann
Release : 2013-10-22
ISBN : 148316280X
Language : En, Es, Fr & De

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Book Description :

Solid-Liquid Separation, Third Edition reviews the equipment and principles involved in the separation of solids and liquids from a suspension. Some important aspects of solid-liquid separation such as washing, flotation, membrane separation, and magnetic separation are discussed. This book is comprised of 23 chapters and begins with an overview of solid-liquid separation processes and the principles involved, including flotation, gravity sedimentation, cake filtration, and deep bed filtration. The following chapters focus on the characterization of particles suspended in liquids; the efficiency of separation of particles from fluids; coagulation and flocculation; gravity thickening; and the operating characteristics, optimum design criteria, and applications of hydrocyclones. The reader is also introduced to various solid-liquid separation processes such as centrifugal sedimentation, screening, and filtration, along with the use of filter aids. Countercurrent washing of solids and problems associated with fine particle recycling are also considered. The final chapter is devoted to the thermodynamics of particle-fluid interaction. This monograph will be useful to chemical engineers and process engineers, particularly those in plant operation, plant design, or equipment testing and commissioning. It can also be used as a textbook for both undergraduate and postgraduate students.

Food Engineering Handbook

Food Engineering Handbook Book
Author : Theodoros Varzakas,Constantina Tzia
Publisher : CRC Press
Release : 2014-11-24
ISBN : 1482261669
Language : En, Es, Fr & De

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Book Description :

Food Engineering Handbook: Food Process Engineering addresses the basic and applied principles of food engineering methods used in food processing operations around the world. Combining theory with a practical, hands-on approach, this book examines the thermophysical properties and modeling of selected processes such as chilling, freezing, and dehydration. A complement to Food Engineering Handbook: Food Engineering Fundamentals, this text: Discusses size reduction, mixing, emulsion, and encapsulation Provides case studies of solid–liquid and supercritical fluid extraction Explores fermentation, enzymes, fluidized-bed drying, and more Presenting cutting-edge information on new and emerging food engineering processes, Food Engineering Handbook: Food Process Engineering is an essential reference on the modeling, quality, safety, and technologies associated with food processing operations today.

Advanced Methods in Molecular Biology and Biotechnology

Advanced Methods in Molecular Biology and Biotechnology Book
Author : Khalid Z. Masoodi,Sameena Maqbool Lone,Rovidha Saba Rasool
Publisher : Academic Press
Release : 2020-11-10
ISBN : 012824450X
Language : En, Es, Fr & De

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Book Description :

Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology Features clear, step-by-step instruction for applying the techniques covered Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment

Principles and Techniques of Biochemistry and Molecular Biology

Principles and Techniques of Biochemistry and Molecular Biology Book
Author : Keith Wilson,John Walker
Publisher : Cambridge University Press
Release : 2010-03-04
ISBN : 1107393493
Language : En, Es, Fr & De

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Book Description :

This best-selling undergraduate textbook provides an introduction to key experimental techniques from across the biosciences. It uniquely integrates the theories and practices that drive the fields of biology and medicine, comprehensively covering both the methods students will encounter in lab classes and those that underpin recent advances and discoveries. Its problem-solving approach continues with worked examples that set a challenge and then show students how the challenge is met. New to this edition are case studies, for example, that illustrate the relevance of the principles and techniques to the diagnosis and treatment of individual patients. Coverage is expanded to include a section on stem cells, chapters on immunochemical techniques and spectroscopy techniques, and additional chapters on drug discovery and development, and clinical biochemistry. Experimental design and the statistical analysis of data are emphasised throughout to ensure students are equipped to successfully plan their own experiments and examine the results obtained.

Separations for Biotechnology 2

Separations for Biotechnology 2 Book
Author : D. Leo Pyle
Publisher : Springer Science & Business Media
Release : 2012-12-06
ISBN : 9400907834
Language : En, Es, Fr & De

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Book Description :

The challenge of bioseparations is to isolate and purify identified products from the dilute product broth produced from cell culture. Innovation in bioseparations technology is increasingly driven by the requirements imposed by the growing importance of production on a process scale of injectable-grade products, and economic pressures to improve the efficiency of downstream processing. As in other areas of technical change, science does not necessarily precede new technology: progress results from a complex and messy mixture of advances in understanding, ingenious ideas, novel techniques and chance discoveries. What is certain is that close interaction between academics and practitioners, biological scientists and process engineers is needed to solve the problems of bioseparations. The Second International Conference on Separations for Biotechnology at Reading, UK, in September 1990 set out to provide a critical multidisciplinary forum for the discussion of bioseparations. This volume contains the papers presented at the meeting. The meeting was organised around six themes with oral and poster presentations on the science and practice of bioseparations technology, and the same structure has been kept for this book. We have also included the texts of the keynote review paper by Professor Alan Michaels and the introductory review papers specially commissioned for the conference. Within each part of this book the review paper is followed by the contributed papers grouped alphabetically by their first author. All the original papers published here were accepted for publication after scientific refereeing.

Countercurrent Chromatography

Countercurrent Chromatography Book
Author : Jean-Michel Menet,Didier Thiebaut
Publisher : CRC Press
Release : 1999-07-29
ISBN : 1482273802
Language : En, Es, Fr & De

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Book Description :

Covering definitions, concepts, and applications, Countercurrent Chromatography recounts the developments in two types of liquid-liquid chromatography termed countercurrent-high-speed countercurrent chromatography (HSCCC) and centrifugal partition chromatography (CPC)-as well as the HSCCC-derived cross-axis CCC, a versatile technique for purificati

Bioseparations Science and Engineering

Bioseparations Science and Engineering Book
Author : Roger G. Harrison,Paul W. Todd,Scott R. Rudge,Demetri P. Petrides
Publisher : Oxford University Press
Release : 2015-01-27
ISBN : 0190213736
Language : En, Es, Fr & De

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Book Description :

Designed for undergraduates, graduate students, and industry practitioners, Bioseparations Science and Engineering fills a critical need in the field of bioseparations. Current, comprehensive, and concise, it covers bioseparations unit operations in unprecedented depth. In each of the chapters, the authors use a consistent method of explaining unit operations, starting with a qualitative description noting the significance and general application of the unit operation. They then illustrate the scientific application of the operation, develop the required mathematical theory, and finally, describe the applications of the theory in engineering practice, with an emphasis on design and scaleup. Unique to this text is a chapter dedicated to bioseparations process design and economics, in which a process simular, SuperPro Designer® is used to analyze and evaluate the production of three important biological products. New to this second edition are updated discussions of moment analysis, computer simulation, membrane chromatography, and evaporation, among others, as well as revised problem sets. Unique features include basic information about bioproducts and engineering analysis and a chapter with bioseparations laboratory exercises. Bioseparations Science and Engineering is ideal for students and professionals working in or studying bioseparations, and is the premier text in the field.

Bioseparation Engineering

Bioseparation Engineering Book
Author : I. Endo,T. Nagamune,S. Katoh,T. Yonemoto
Publisher : Elsevier
Release : 2000-03-17
ISBN : 9780080528151
Language : En, Es, Fr & De

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Book Description :

The bioseparation engineering of today includes downstream process engineering such as waste water, material and gas treatment. Taking this tendency into account, bioseparation engineers gathered in Japan as a special research group under the main theme of "Recovery and Recycle of Resources to Protect the Global Environment". The scope of this book is based on the conference, and deals not only with recent advances in bioseparation engineering in a narrow sence, but also the environmental engineering which includes waste water treatment and bioremediation. The contributors of this book cover many disciplines such as chemical engineering, analytical chemistry, biochemistry, and microbiology. Bioseparation Engineering will stimulate young engineers and scientists who will develop bioseparation engineering further in the 21st century, and contribute to a world-wide attention to the global environment

Introduction to Instrumentation in Life Sciences

Introduction to Instrumentation in Life Sciences Book
Author : Prakash Singh Bisen,Anjana Sharma
Publisher : CRC Press
Release : 2012-09-26
ISBN : 1466512407
Language : En, Es, Fr & De

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Book Description :

Instrumentation is central to the study of physiology and genetics in living organisms, especially at the molecular level. Numerous techniques have been developed to address this in various biological disciplines, creating a need to understand the physical principles involved in the operation of research instruments and the parameters required in using them. Introduction to Instrumentation in Life Sciences fills this need by addressing different aspects of tools that hold the keys to cutting-edge research and innovative applications, from basic techniques to advanced instrumentation. The text describes all topics so even beginners can easily understand the theoretical and practical aspects. Comprehensive chapters encompass well-defined methodology that describes the instruments and their corresponding applications in different scientific fields. The book covers optical and electron microscopy; micrometry, especially in microbial taxonomy; pH meters and oxygen electrodes; chromatography for separation and purification of products from complex mixtures; spectroscopic and spectrophotometric techniques to determine structure and function of biomolecules; preparative and analytical centrifugation; electrophoretic techniques; x-ray microanalysis including crystallography; applications of radioactivity, including autoradiography and radioimmunoassays; and fermentation technology and subsequent separation of products of interest. The book is designed to serve a wide range of students and researchers in diversified fields of life sciences: pharmacy, biotechnology, microbiology, biochemistry, and environmental sciences. It introduces different aspects of basic experimental methods and instrumentation. The book is unique in its broad subject coverage, incorporating fundamental techniques as well as applications of modern molecular and proteomic tools that are the basis for state-of-the-art research. The text emphasizes techniques encountered both in practical classes and in high-throughput environments used in modern industry. As a further aid to students, the authors provide well-illustrated diagrams to explain the principles and theories behind the instruments described.

Industrial Separation Processes

Industrial Separation Processes Book
Author : André B. de Haan,H. Burak Eral,Boelo Schuur
Publisher : Walter de Gruyter GmbH & Co KG
Release : 2020-07-06
ISBN : 3110654806
Language : En, Es, Fr & De

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Book Description :

Separation processes on an industrial scale account for well over half of the capital and operating costs in the chemical industry. Knowledge of these processes is key for every student of chemical or process engineering. This book is ideally suited to university teaching, thanks to its wealth of exercises and solutions. The second edition boasts an even greater number of applied examples and case studies as well as references for further reading.

Wet Cake Filtration

Wet Cake Filtration Book
Author : Harald Anlauf
Publisher : John Wiley & Sons
Release : 2019-10-14
ISBN : 3527346066
Language : En, Es, Fr & De

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Book Description :

Guides readers through the entire process of liquid filtrations, from a basic understanding and lab scale testing to advanced process applications and up-scaling of processes Wet Cake Filtration is a key method in solid-liquid separation and plays an important role in many industrial processes from the separation of solid products from a liquid, to removing contaminants in wastewater treatment. Furthermore, separation processes are rarely isolated and the integration as well as necessary pre-treatments in the process chain must be carefully considered and implemented. Supported by more than 40 years of research, development, and teaching, this book provides a comprehensive treatment of all relevant aspects in wet cake filtration as a key method in solid-liquid-separation. The first part of Wet Cake Filtration: Fundamentals, Equipment, Strategies discusses general principles and applications of wet cake filtration, determination of proper feed streams, and filter cake formation. The next chapters deal with variations of pre-treatment and process conditions, including necessary aspects of lab scale tests, up-scaling, and filter design. This is further strengthened with chapters examining particle purification, yield maximization, and cake deliquoring. Lastly, the filter media is discussed as the central piece of wet cake filtration. Beside the different possibilities of available filter media structures and process relevant aspects of filter media selection, the reliable characterization of pore sizes by porometry and innovative additional functionalities are introduced. -Provides information on wet cake filtration?the necessary pre-treatments and process considerations?to guide the reader to develop or improve their own processes -Offers the necessary tools that allow the engineer to transform a lab scale test into a scaled-up process -Presents cake filtration process-related topics like slurry characterization or slurry pretreatment, and special developments such as hyperbaric filtration or steam pressure filtration -Discusses promising new processes like gasless cake desaturation and shrinkage crack free cake desaturation Wet Cake Filtration is a must-have resource for every engineer working with wet cake filtration, including water chemists, catalytic chemists, food chemists, chemical engineers, biotechnologists, and process engineers.

Membrane Separations in Biotechnology

Membrane Separations in Biotechnology Book
Author : McGregor
Publisher : Marcel Dekker Incorporated
Release : 1986
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Membrane Separations in Biotechnology book written by McGregor, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Industrial Centrifugation Technology

Industrial Centrifugation Technology Book
Author : Wallace Woon-Fong Leung
Publisher : McGraw Hill Professional
Release : 1998
ISBN : 9780070371910
Language : En, Es, Fr & De

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Book Description :

Introduction General Principles of Sedimenting Centrifuges Batch Sedimenting Centrifuges Continous Sedimenting Centrifuges Applications of Sedimenting Centrifuges Continous-Feed Sedimentation General Principles of Filtering Centrifuges Batch Filtering Centrifuges Continuous Filtering Centrifuges Applications of Filtering Centrifuges Feed Acceleration Lab, Pilot, and Production Tests Centrifuge Selection and Sizing Optimization and Troubleshooting Kaolin Processing Dewatering of Compactible Solids Cake compaction theory Appendices Name Index Subject Index.

Downstream Processing of Proteins

Downstream Processing of Proteins Book
Author : Mohamed A. Desai
Publisher : Springer Science & Business Media
Release : 2000
ISBN : 1592590276
Language : En, Es, Fr & De

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Book Description :

In Downstream Processing of Proteins: Methods and Protocols, Mohamed A. Desai and a team of experienced biotechnologists review both conventional and novel isolation techniques used in industrial applications for the downstream processing of protein molecules. These techniques include primary and secondary separations during the isolation of biomolecules, as well as unique laboratory-scale research methods with a potential for scale-up. Also treated are the various strands of the downstream biological process essential for a successful product license application, including both the validation of DSP stages, and the design and validation of viral clearance stages during the purification process. Downstream Processing of Proteins: Methods and Protocols provides scientists everywhere, but particularly in the biopharmaceutical and biotechnology industry, with a much-needed introduction to this critical technology. Every bioprocess scientist and engineer working to design and validate biological processes for novel proteins-and successfully apply for their new product licenses-will find this important book an eminently practical resource.

Membrane Separations in Biotechnology

Membrane Separations in Biotechnology Book
Author : William K. Wang
Publisher : CRC Press
Release : 2001-04-03
ISBN : 1482289881
Language : En, Es, Fr & De

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Book Description :

This text details the relationship between membrane technology and bioprocesses, discussing applications. This second edition refines and optimizes key features of the first edition - and features new illustrative case studies. The book examines advantages and disadvantages of using standard and new membrane technologies; analyzes a wide range of a

Handbook of Downstream Processing

Handbook of Downstream Processing Book
Author : E. Goldberg
Publisher : Springer Science & Business Media
Release : 2012-12-06
ISBN : 9400915632
Language : En, Es, Fr & De

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Book Description :

The last two decades have seen a phenomenal growth of the field of genetic or biochemical engineering and have witnessed the development and ultimately marketing of a variety of products-typically through the manipulation and growth of different types of microorganisms, followed by the recovery and purification of the associated products. The engineers and biotechnologists who are involved in the full-scale process design of such facilities must be familiar with the variety of unit operations and equipment and the applicable regulatory requirements. This book describes current commercial practice and will be useful to those engineers working in this field in the design, construction and operation of pharmaceutical and biotechnology plants. It will be of help to the chemical or pharmaceutical engineer who is developing a plant design and who faces issues such as: Should the process be batch or continuous or a combination of batch and continuous? How should the optimum process design be developed? Should one employ a new revolutionary separation which could be potentially difficult to validate or use accepted technology which involves less risk? Should the process be run with ingredients formulated from water for injection, deionized water, or even filtered tap water? Should any of the separations be run in cold rooms or in glycol jacketed lines to minimize microbial growth where sterilization is not possible? Should the process equipment and lines be designed to be sterilized in-place, cleaned-in-place, or should every piece be broken down, cleaned and autoclaved after every turn?

Calculations for Molecular Biology and Biotechnology

Calculations for Molecular Biology and Biotechnology Book
Author : Frank H. Stephenson
Publisher : Academic Press
Release : 2010-07-30
ISBN : 9780123756916
Language : En, Es, Fr & De

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Book Description :

Calculations for Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory, Second Edition, provides an introduction to the myriad of laboratory calculations used in molecular biology and biotechnology. The book begins by discussing the use of scientific notation and metric prefixes, which require the use of exponents and an understanding of significant digits. It explains the mathematics involved in making solutions; the characteristics of cell growth; the multiplicity of infection; and the quantification of nucleic acids. It includes chapters that deal with the mathematics involved in the use of radioisotopes in nucleic acid research; the synthesis of oligonucleotides; the polymerase chain reaction (PCR) method; and the development of recombinant DNA technology. Protein quantification and the assessment of protein activity are also discussed, along with the centrifugation method and applications of PCR in forensics and paternity testing. Topics range from basic scientific notations to complex subjects like nucleic acid chemistry and recombinant DNA technology Each chapter includes a brief explanation of the concept and covers necessary definitions, theory and rationale for each type of calculation Recent applications of the procedures and computations in clinical, academic, industrial and basic research laboratories are cited throughout the text New to this Edition: Updated and increased coverage of real time PCR and the mathematics used to measure gene expression More sample problems in every chapter for readers to practice concepts

Bioanalytical Techniques

Bioanalytical Techniques Book
Author : Abhilasha Shourie
Publisher : The Energy and Resources Institute (TERI)
Release : 2005-01-01
ISBN : 8179935299
Language : En, Es, Fr & De

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Book Description :

Bioanalytical Techniques form an integral part of applied biology and biomedical sciences. The various principles of bioanalytical techniques used in biomedical sciences, environmental studies, life sciences, pharmaceutical analysis, molecular biology, and biotechnological research are comprehensively discussed in this book. Analytical instrumentation is also explained in as concise a manner as possible. Microscopy, centrifugation, chromatography, electrophoresis, spectroscopy, and radioisotope and immunodiagnostic techniques are the main topics focussed in this book. Techniques in molecular biology and recombinant DNA technology have also been described in detail.

Chromatographic and Membrane Processes in Biotechnology

Chromatographic and Membrane Processes in Biotechnology Book
Author : C.A. Costa,Joaquim S. Cabral
Publisher : Springer Science & Business Media
Release : 2012-12-06
ISBN : 9401134707
Language : En, Es, Fr & De

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Book Description :

Separation processes in biotechnology are of increasing industrial importance since they entail the major costs of bioprocessing especially when high purity is required. Chromatography and membranes are two of the most important technologies used for direct treatment of fermentation broths as well as for high resolution steps in product purification. The theoretical foundations of chromatographic and membrane processes are well understood for the case of small molecules. Nevertheless there is a need to adapt and further develop that knowledge to the processing of large biological molecules. This is being achieved with the contribution of other areas like molecular biology and materials science. The objective of this NATO Advanced Study Institute is to present an updated treatment of the fundamentals of chromatographic and membrane processes with special relevance in bioprocessing.This volume collects the lectures presented at this Institute. The lectures are arranged in five chapters. Chapter I deals with chromatographic processes covering topics like equilibrium, kinetics and contacting devices. Membrane processes and some applications in biotechnology are treated in chapter 2. Chapter 3 is devoted to affinity chromatographic and membrane processes. Chapter 4 considers the current developments on chromatographic supports and membranes both from the constitutive materials and form points of view. Scale-up, optimization and reaction/separation integration are the topics covered in chapter 5. We are very grateful to all lecturers and participants that made possible this Institute. Financial support from NATO Scientific Affairs Division, INIC, JNICT, FLAD, University of Ac;ores and DRT Ac;ores is gratefully acknowledged.