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Cellular Imaging Techniques For Neuroscience And Beyond

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Cellular Imaging Techniques for Neuroscience and Beyond

Cellular Imaging Techniques for Neuroscience and Beyond Book
Author : Floris G. Wouterlood
Publisher : Academic Press
Release : 2012
ISBN : 0123858720
Language : En, Es, Fr & De

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Book Description :

The imaging of small cellular components requires powerful instruments, and an entire family of equipment and techniques based on the confocal principle has been developed over the past 30 years. Such methods are commonly used by neuroscience researchers, but the majority of these users do not have a microscopy or a cell biology backgrounds and do can encounter difficulties in obtaining and interpreting results. This volume brings experts in high-resolution optical microscopy applications in neuroscience and cell biology together to document the state of the art. Outlining what is currently possible, the volume also discusses promising developments for the future and aids readers in selecting the most scientifically meaningful approach to solve their questions. Each chapter discusses instrumentation and technology in relationship to application in research. All of the common and cutting edge trends are covered - fluorescence / laser electron / nonlinear microscopy, infrared fluorescence, multiphoton imaging, tomography, FRAP, live imaging, STED, PALM/STORM, etc. * The first comprehensive volume on cellular imaging with a focus for its application in neuroscience * Concluding chapter compares the merits of various techniques * Full color throughout, maximizing users comprehension of the results obtainable via various methods * Features outstanding and truly international scholarship, with chapters written by leading experts in neuroscience and cell biology * Discusses cutting edge methods such as STED, PALM/STORM, nonlinear microscopy and more

Cellular Imaging Techniques for Neuroscience and Beyond

Cellular Imaging Techniques for Neuroscience and Beyond Book
Author : Floris G. Wouterlood
Publisher : Academic Press
Release : 2012-12-06
ISBN : 0123858739
Language : En, Es, Fr & De

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Book Description :

The imaging of small cellular components requires powerful instruments, and an entire family of equipment and techniques based on the confocal principle has been developed over the past 30 years. Such methods are commonly used by neuroscience researchers, but the majority of these users do not have a microscopy or a cell biology backgrounds and do can encounter difficulties in obtaining and interpreting results. This volume brings experts in high-resolution optical microscopy applications in neuroscience and cell biology together to document the state of the art. Outlining what is currently possible, the volume also discusses promising developments for the future and aids readers in selecting the most scientifically meaningful approach to solve their questions. Each chapter discusses instrumentation and technology in relationship to application in research. All of the common and cutting edge trends are covered - fluorescence / laser electron / nonlinear microscopy, infrared fluorescence, multiphoton imaging, tomography, FRAP, live imaging, STED, PALM/STORM, etc. Single and multiphoton confocal microscopy, and 4-pi confocal microscopy Obtaining nanoresolution via photoactivation localization microscopy (PALM) Several procedures that correlate observations in optical fluorescence microscopy and electron microscopy Study of morphology and function via high-resolution fluorescence procedures Additional high-resolution microscopic techniques

Optical Probes in Biology

Optical Probes in Biology Book
Author : Jin Zhang,Sohum Mehta,Carsten Schultz
Publisher : CRC Press
Release : 2016-04-19
ISBN : 1466510129
Language : En, Es, Fr & De

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Book Description :

Optical probes, particularly the fluorescent varieties, enable researchers to observe cellular events in real time and with great spatial resolution. Optical Probes in Biology explores the diverse capabilities of these powerful and versatile tools and presents various approaches used to design, develop, and implement them. The book examines the use of optical probes to detect and track numerous molecular processes in living cells, including GTPase and kinase activities, membrane lipids, voltage, metal ions, metabolic signals, RNA, and histone modifications. It critically reviews the different probe designs and delves into the strategies for developing new fluorescent protein varieties with enhanced capabilities. It also covers sophisticated imaging techniques and equipment, such as intensity and lifetime-based fluorescence microscopy methods, used to visualize and track optical probes. In addition, the book goes beyond live-cell tracking to discuss the growing application of activity-based probes for performing pharmacological drug screening and probing molecular processes in living animals. It also discusses emerging techniques that are expanding optical probe-based approaches into new biological frontiers. With contributions from top international scientists, this book offers a thorough overview of the latest optical probes in cell biology and biochemistry. Both newcomers and established researchers will discover how to incorporate state-of-the-art optical probes and fluorescence imaging into their research.

Neurohistology and Imaging Techniques

Neurohistology and Imaging Techniques Book
Author : Radek Pelc,Wolfgang Walz,Ronald Doucette
Publisher : Humana
Release : 2020-06-25
ISBN : 9781071604267
Language : En, Es, Fr & De

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Book Description :

This volume explores major light microscopic imaging modalities that can be used to view nervous tissue, and discusses the steps needed to use each of them, and ways to interpret the data. The chapters in this book cover topics such as atlasing of insect brain; neuroanatomical tracing through fluorochrome expression; fluorescent probes for amyloids; or optical clearing for ultramicroscopy of GFP-expressing tissues. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Authoritative and cutting-edge, Neurohistology and Imaging Techniques is a valuable resource for both expert and novice users of major light microscopic imaging techniques, and those interested in exploring alternate imaging tools.

Optogenetics

Optogenetics Book
Author : Hiromu Yawo,Hideki Kandori,Amane Koizumi,Ryoichiro Kageyama
Publisher : Springer Nature
Release : 2021
ISBN : 9811587639
Language : En, Es, Fr & De

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Book Description :

This book, now in a thoroughly revised second edition, offers a comprehensive review of the rapidly growing field of optogenetics, in which light-sensing proteins are genetically engineered into cells in order to acquire information on cellular physiology in optical form or to enable control of specific network in the brain upon activation by light. Light-sensing proteins of various living organisms are now available to be exogenously expressed in neurons and other target cells both in vivo and in vitro. Cellular functions can thus be manipulated or probed by light. The new edition documents fully the extensive progress since publication of the first edition to provide an up-to-date overview of the physical, chemical, and biological properties of light-sensing proteins and their application in biological systems, particularly in neuroscience but also in medicine and the optical sciences. Underlying principles are explained and detailed information provided on a wide range of optogenetic tools for the observation and control of cellular signaling and physiology, gene targeting technologies, and optical methods for biological applications. In presenting the current status of optogenetics and emerging directions, this milestone publication will be a "must read" for all involved in research in any way related to optogenetics.

Visualizing Chemistry

Visualizing Chemistry Book
Author : National Research Council,Division on Earth and Life Studies,Board on Chemical Sciences and Technology,Committee on Revealing Chemistry through Advanced Chemical Imaging
Publisher : National Academies Press
Release : 2006-06-01
ISBN : 9780309164634
Language : En, Es, Fr & De

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Book Description :

Scientists and engineers have long relied on the power of imaging techniques to help see objects invisible to the naked eye, and thus, to advance scientific knowledge. These experts are constantly pushing the limits of technology in pursuit of chemical imaging—the ability to visualize molecular structures and chemical composition in time and space as actual events unfold—from the smallest dimension of a biological system to the widest expanse of a distant galaxy. Chemical imaging has a variety of applications for almost every facet of our daily lives, ranging from medical diagnosis and treatment to the study and design of material properties in new products. In addition to highlighting advances in chemical imaging that could have the greatest impact on critical problems in science and technology, Visualizing Chemistry reviews the current state of chemical imaging technology, identifies promising future developments and their applications, and suggests a research and educational agenda to enable breakthrough improvements.

In Vivo Optical Imaging of Brain Function Second Edition

In Vivo Optical Imaging of Brain Function  Second Edition Book
Author : Ron D. Frostig
Publisher : CRC Press
Release : 2009-05-06
ISBN : 142007685X
Language : En, Es, Fr & De

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Book Description :

These are exciting times for the field of optical imaging of brain function. Rapid developments in theory and technology continue to considerably advance understanding of brain function. Reflecting changes in the field during the past five years, the second edition of In Vivo Optical Imaging of Brain Function describes state-of-the-art techniques and their applications for the growing field of functional imaging in the live brain using optical imaging techniques. New in the Second Edition: Voltage-sensitive dyes imaging in awake behaving animals Imaging based on genetically encoded probes Imaging of mitochondrial auto-fluorescence as a tool for cortical mapping Using pH-sensitive dyes for functional mapping Modulated imaging Calcium imaging of neuronal activity using 2-photon microscopy Fourier approach to optical imaging Fully updated chapters from the first edition Leading Authorities Explore the Latest Techniques Updated to reflect continuous development in this emerging research area, this new edition, as with the original, reaches across disciplines to review a variety of non-invasive optical techniques used to study activity in the living brain. Leading authorities from such diverse areas as biophysics, neuroscience, and cognitive science present a host of perspectives that range from a single neuron to large assemblies of millions of neurons, captured at various temporal and spatial resolutions. Introducing techniques that were not available just a few years ago, the authors describe the theory, setup, analytical methods, and examples that highlight the advantages of each particular method.

Optogenetics

Optogenetics Book
Author : Sophie Vriz,Takeaki Ozawa
Publisher : Royal Society of Chemistry
Release : 2018-09-18
ISBN : 1788012372
Language : En, Es, Fr & De

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Book Description :

Optogenetic tools have allowed significant advances in the understanding of biological problems, particularly in the neurosciences field. Biological tools as well as optical set-ups have evolved and a wide range of probes and light-controllable modules are now available. The aim of this book is to give a flavour of illumination strategies and imaging with an overview of the different optogenetic tools and their main applications in cell biology. Based on examples covering the different aspects of cell biology, this book provides a practical approach for using light-emitting sensors and light-driven actuators.

Live Cell Imaging

Live Cell Imaging Book
Author : Robert D. Goldman,David L. Spector
Publisher : CSHL Press
Release : 2005
ISBN : 9780879696832
Language : En, Es, Fr & De

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Book Description :

Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a DVD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book. The editors, David Spector and Robert Goldman, whose previous book was Cells: A Laboratory Manual,are highly respected investigators who have taught microscopy courses at Cold Spring Harbor Laboratory, the Marine Biology Laboratory at Woods Hole, and Northwestern University.

Discovering the Brain

Discovering the Brain Book
Author : National Academy of Sciences,Institute of Medicine,Sandra Ackerman
Publisher : National Academies Press
Release : 1992-01-01
ISBN : 0309045290
Language : En, Es, Fr & De

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Book Description :

The brain ... There is no other part of the human anatomy that is so intriguing. How does it develop and function and why does it sometimes, tragically, degenerate? The answers are complex. In Discovering the Brain, science writer Sandra Ackerman cuts through the complexity to bring this vital topic to the public. The 1990s were declared the "Decade of the Brain" by former President Bush, and the neuroscience community responded with a host of new investigations and conferences. Discovering the Brain is based on the Institute of Medicine conference, Decade of the Brain: Frontiers in Neuroscience and Brain Research. Discovering the Brain is a "field guide" to the brain--an easy-to-read discussion of the brain's physical structure and where functions such as language and music appreciation lie. Ackerman examines How electrical and chemical signals are conveyed in the brain. The mechanisms by which we see, hear, think, and pay attention--and how a "gut feeling" actually originates in the brain. Learning and memory retention, including parallels to computer memory and what they might tell us about our own mental capacity. Development of the brain throughout the life span, with a look at the aging brain. Ackerman provides an enlightening chapter on the connection between the brain's physical condition and various mental disorders and notes what progress can realistically be made toward the prevention and treatment of stroke and other ailments. Finally, she explores the potential for major advances during the "Decade of the Brain," with a look at medical imaging techniques--what various technologies can and cannot tell us--and how the public and private sectors can contribute to continued advances in neuroscience. This highly readable volume will provide the public and policymakers--and many scientists as well--with a helpful guide to understanding the many discoveries that are sure to be announced throughout the "Decade of the Brain."

Guide to Research Techniques in Neuroscience

Guide to Research Techniques in Neuroscience Book
Author : Matt Carter,Rachel Essner,Nitsan Goldstein,Manasi Iyer
Publisher : Academic Press
Release : 2022-04-08
ISBN : 0323915612
Language : En, Es, Fr & De

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Book Description :

Modern neuroscience research is inherently multidisciplinary, with a wide variety of cutting edge new techniques to explore multiple levels of investigation. This Third Edition of Guide to Research Techniques in Neuroscience provides a comprehensive overview of classical and cutting edge methods including their utility, limitations, and how data are presented in the literature. This book can be used as an introduction to neuroscience techniques for anyone new to the field or as a reference for any neuroscientist while reading papers or attending talks. • Nearly 200 updated full-color illustrations to clearly convey the theory and practice of neuroscience methods • Expands on techniques from previous editions and covers many new techniques including in vivo calcium imaging, fiber photometry, RNA-Seq, brain spheroids, CRISPR-Cas9 genome editing, and more • Clear, straightforward explanations of each technique for anyone new to the field • A broad scope of methods, from noninvasive brain imaging in human subjects, to electrophysiology in animal models, to recombinant DNA technology in test tubes, to transfection of neurons in cell culture • Detailed recommendations on where to find protocols and other resources for specific techniques • “Walk-through boxes that guide readers through experiments step-by-step

From Neurons to Neighborhoods

From Neurons to Neighborhoods Book
Author : Division of Behavioral and Social Sciences and Education,Institute of Medicine,Board on Children, Youth, and Families,Committee on Integrating the Science of Early Childhood Development
Publisher : National Academies Press
Release : 2000-11-13
ISBN : 0309069882
Language : En, Es, Fr & De

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Book Description :

How we raise young children is one of today's most highly personalized and sharply politicized issues, in part because each of us can claim some level of "expertise." The debate has intensified as discoveries about our development-in the womb and in the first months and years-have reached the popular media. How can we use our burgeoning knowledge to assure the well-being of all young children, for their own sake as well as for the sake of our nation? Drawing from new findings, this book presents important conclusions about nature-versus-nurture, the impact of being born into a working family, the effect of politics on programs for children, the costs and benefits of intervention, and other issues. The committee issues a series of challenges to decision makers regarding the quality of child care, issues of racial and ethnic diversity, the integration of children's cognitive and emotional development, and more. Authoritative yet accessible, From Neurons to Neighborhoods presents the evidence about "brain wiring" and how kids learn to speak, think, and regulate their behavior. It examines the effect of the climate-family, child care, community-within which the child grows.

Microscopy Techniques

Microscopy Techniques Book
Author : Jens Rietdorf
Publisher : Springer Science & Business Media
Release : 2005-06-23
ISBN : 9783540236986
Language : En, Es, Fr & De

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Book Description :

With contributions by numerous experts

Neuroethics

Neuroethics Book
Author : Judy Illes
Publisher : Oxford University Press
Release : 2017-07-20
ISBN : 0198786832
Language : En, Es, Fr & De

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Book Description :

Over the last decade, there have been unparalleled advances in our understanding of brain sciences. But with the development of tools that can manipulate brain function, there are pressing ethical implications to this newfound knowledge of how the brain works. In Neuroethics: Anticipating theFuture, a distinguished group of contributors tackle current and critical ethical questions and offer forward-looking insights. What new balances should be struck between diagnosis and prediction, or invasive and non-invasive interventions, given the rapid advances in neuroscience? Are new criteria needed for the clinical definition of death for those eligible for organ donation? As data from emerging technologies are madeavailable on public databases, what frameworks will maximize benefits while ensuring privacy of health information? These challenging questions, along with numerous other neuroethical concerns, are discussed in depth. Written by eminent scholars from diverse disciplines including neurology and neuroscience, ethics and law, public health and philosophy, this new volume on neuroethics sets out the many necessary considerations for the future. It is essential reading for the field of neuroethics, neurosciences andpsychology, and an invaluable resource for physicians in neurological medicine, academics in humanities and law, and health policy makers.

Fundamentals of Light Microscopy and Electronic Imaging

Fundamentals of Light Microscopy and Electronic Imaging Book
Author : Douglas B. Murphy,Michael W. Davidson
Publisher : John Wiley & Sons
Release : 2012-08-22
ISBN : 1118382935
Language : En, Es, Fr & De

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Book Description :

Fundamentals of Light Microscopy and Electronic Imaging, Second Edition provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags. The book is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. This revision includes new chapters on live cell imaging, measurement of protein dynamics, deconvolution microscopy, and interference microscopy. PowerPoint slides of the figures as well as other supplementary materials for instructors are available at a companion website: www.wiley.com/go/murphy/lightmicroscopy

Electrochemical Methods for Neuroscience

Electrochemical Methods for Neuroscience Book
Author : Adrian C. Michael,Laura Borland
Publisher : CRC Press
Release : 2006-12-13
ISBN : 1420005863
Language : En, Es, Fr & De

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Book Description :

Since the first implant of a carbon microelectrode in a rat 35 years ago, there have been substantial advances in the sensitivity, selectivity and temporal resolution of electrochemical techniques. Today, these methods provide neurochemical information that is not accessible by other means. The growing recognition of the versatility of electrochemical techniques indicates a need for a greater understanding of the scientific foundation and use of these powerful tools. Electrochemical Methods for Neuroscience provides an updated summary of the current, albeit evolving, state of the art and lays the scientific foundation for incorporating electrochemical techniques into on-going or newly emerging research programs in the neuroscience disciplines. With contributions from pioneers in the field, the text outlines the applications and benefits of a wide range of electrochemical techniques. It explores the methodology behind the acquisition of neurochemical and neurobiological data through continuous amperometry, fast scan cyclic voltammetry, high-speed chronoamperometry, ion-selective microelectrodes, enzyme based microelectrodes, and in vivo voltammetry with telemetry. The text also introduces emerging concepts in the field such as the correlation of electrochemical recordings with information obtained from patch clamp, electrophysiological, and behavioral techniques. By presenting up-to-date information on the growing collection of electrochemical methods, microsensors, and research techniques, Electrochemical Methods for Neuroscience assists seasoned researchers and newcomers to the field in making sound decisions about adopting the most appropriate of these tools for their future research objectives.

Quantitative Imaging in Cell Biology

Quantitative Imaging in Cell Biology Book
Author : Anonim
Publisher : Academic Press
Release : 2014-06-25
ISBN : 0124202012
Language : En, Es, Fr & De

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Book Description :

This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material

Network Neuroscience

Network Neuroscience Book
Author : Flavio Fröhlich
Publisher : Academic Press
Release : 2016-09-20
ISBN : 0128015861
Language : En, Es, Fr & De

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Book Description :

Studying brain networks has become a truly interdisciplinary endeavor, attracting students and seasoned researchers alike from a wide variety of academic backgrounds. What has been lacking is an introductory textbook that brings together the different fields and provides a gentle introduction to the major concepts and findings in the emerging field of network neuroscience. Network Neuroscience is a one-stop-shop that is of equal use to the neurobiologist, who is interested in understanding the quantitative methods employed in network neuroscience, and to the physicist or engineer, who is interested in neuroscience applications of mathematical and engineering tools. The book spans 27 chapters that cover everything from individual cells all the way to complex network disorders such as depression and autism spectrum disorders. An additional 12 toolboxes provide the necessary background for making network neuroscience accessible independent of the reader’s background. Dr. Flavio Frohlich (www.networkneuroscientist.org) wrote this book based on his experience of mentoring dozens of trainees in the Frohlich Lab, from undergraduate students to senior researchers. The Frohlich lab (www.frohlichlab.org) pursues a unique and integrated vision that combines computer simulations, animal model studies, human studies, and clinical trials with the goal of developing novel brain stimulation treatments for psychiatric disorders. The book is based on a course he teaches at UNC that has attracted trainees from many different departments, including neuroscience, biomedical engineering, psychology, cell biology, physiology, neurology, and psychiatry. Dr. Frohlich has consistently received rave reviews for his teaching. With this book he hopes to make his integrated view of neuroscience available to trainees and researchers on a global scale. His goal is to make the book the training manual for the next generation of (network) neuroscientists, who will be fusing biology, engineering, and medicine to unravel the big questions about the brain and to revolutionize psychiatry and neurology. Easy-to-read, comprehensive introduction to the emerging field of network neuroscience Includes 27 chapters packed with information on topics from single neurons to complex network disorders such as depression and autism Features 12 toolboxes serve as primers to provide essential background knowledge in the fields of biology, mathematics, engineering, and physics

Behavioral Neuroscience

Behavioral Neuroscience Book
Author : Stéphane Gaskin
Publisher : SAGE Publications
Release : 2019-12-04
ISBN : 1506391478
Language : En, Es, Fr & De

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Book Description :

Behavioral Neuroscience: Essentials and Beyond shows students the basics of biological psychology using a modern and research-based perspective. With fresh coverage of applied topics and complex phenomena, including social neuroscience and consciousness, author Stéphane Gaskin delivers the most current research and developments surrounding the brain′s functions through student-centered pedagogy. Carefully crafted features introduce students to challenging biological and neuroscience-based concepts through illustrations of real-life application, exploring myths and misconceptions, and addressing students′ assumptions head on. INSTRUCTORS: Behavioral Neuroscience: Essentials and Beyond is accompanied by a complete teaching and learning package! Contact your rep to request a demo. SAGE Premium Video Figures Brought to Life animations in the Interactive eBook boost student comprehension and bolster analysis. Watch a sample video. Interactive eBook Your students save when you bundle the print loose-leaf book with the Interactive eBook (Bundle ISBN: 978-1-0718-1347-8), which includes access to SAGE Premium Video and other multimedia tools. Learn more. SAGE Coursepacks SAGE Coursepacks makes it easy to import our quality instructor and student resource content into your school’s learning management system (LMS). Learn more. SAGE Edge This open-access site offers students an impressive array of learning tools and resources. Learn more.

IBM Systems Journal

IBM Systems Journal Book
Author : International Business Machines Corporation
Publisher : Unknown
Release : 2007
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download IBM Systems Journal book written by International Business Machines Corporation, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.