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Cardiovascular Genome Editing

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Investigating Genetic Risk Factors of Coronary Artery Disease Using Genome Editing

Investigating Genetic Risk Factors of Coronary Artery Disease Using Genome Editing Book
Author : David Graham McVey
Publisher : Unknown
Release : 2016
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Investigating Genetic Risk Factors of Coronary Artery Disease Using Genome Editing book written by David Graham McVey, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Genome Editing

Genome Editing Book
Author : Kursad Turksen
Publisher : Springer
Release : 2016-08-09
ISBN : 3319341480
Language : En, Es, Fr & De

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Book Description :

This timely volume explores the use of CRISPR-Cas9 for genome editing, presenting cutting-edge techniques and their applications in treatment of disease. The chapters describe latest methods such as use of targetable nucleases, investigation of the non-coding genome, mouse genome editing, increasing of knock-in efficiency in mouse zygotes, and generation of reporter stem cells; the text contextualizes these methods in treatment of cardiovascular disease, diabetes mellitus, retinitis pigmentosa, and others. The final chapters round out the book with a discussion of controversies and future directions. Genome Editing is an essential, of-the-moment contribution to this rapidly growing field. Drawing from a wealth of international perspectives, it presents novel techniques and applications for the engineering of the human genome. This book is essential reading for all clinicians and researchers in stem cells, regenerative medicine, genomics, biochemical and biomedical engineering- especially those interested in learning more about genome editing and applying it in a targeted, specific way.

Genome Editing

Genome Editing Book
Author : Kiran Musunuru
Publisher : Academic Press
Release : 2021-03-05
ISBN : 0128236477
Language : En, Es, Fr & De

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Book Description :

Genome Editing: A Practical Guide to Research and Clinical Applications is geared towards investigators interested in learning how to use CRISPR-Cas9-based technologies, with a focus on cardiovascular research and clinical applications. Covering a range of topics from the basics of genome editing to design considerations, to assessments and applications, this reference allows readers to get started and establish a full workflow from the beginning of the project to its full completion. With worked examples drawn from real-life experiments, as well as troubleshooting and pitfalls to avoid, the book serves as an essential reference for researchers and investigators in both cardiovascular and biomedical research. Help readers familiarise with the variety of genome-editing approaches that are being applied in cardiovascular research and medicine, i.e., both research applications and clinical applications Understand the use of genome editing through worked examples (based on real-life experiments) in which CRISPR-Cas9 is employed, online tools to design CRISPR-Cas9 reagents, methods to interpret data from genome-editing experiments, the downsides of genome-editing technology - both the scientific and ethical pitfalls to avoid Written in an easy-to-follow manner, guiding readers from the design of the project to its completion Includes unpublished and new methods

Genome Editing

Genome Editing Book
Author : Kiran Musunuru
Publisher : Elsevier
Release : 2021-03-29
ISBN : 0128234849
Language : En, Es, Fr & De

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Book Description :

Genome Editing: A Practical Guide to Research and Clinical Applications is geared towards investigators interested in learning how to use CRISPR-Cas9-based technologies, with a focus on cardiovascular research and clinical applications. Covering a range of topics from the basics of genome editing to design considerations, to assessments and applications, this reference allows readers to get started and establish a full workflow from the beginning of the project to its full completion. With worked examples drawn from real-life experiments, as well as troubleshooting and pitfalls to avoid, the book serves as an essential reference for researchers and investigators in both cardiovascular and biomedical research. Help readers familiarise with the variety of genome-editing approaches that are being applied in cardiovascular research and medicine, i.e., both research applications and clinical applications Understand the use of genome editing through worked examples (based on real-life experiments) in which CRISPR-Cas9 is employed, online tools to design CRISPR-Cas9 reagents, methods to interpret data from genome-editing experiments, the downsides of genome-editing technology - both the scientific and ethical pitfalls to avoid Written in an easy-to-follow manner, guiding readers from the design of the project to its completion Includes unpublished and new methods

Isogenic Human Pluripotent Stem Cell Models of Cardiovascular Disease Associated Genetic Variation

Isogenic Human Pluripotent Stem Cell Models of Cardiovascular Disease Associated Genetic Variation Book
Author : Derek Tilghman Peters
Publisher : Unknown
Release : 2016
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

The properties of human pluripotent stem cells (hPSCs) make them uniquely suited to address this challenge - they retain a normal human genome in culture, can be genetically modified, and can be differentiated into multiple cell types. The work presented in this thesis demonstrates the use of hPSCs and genome editing technology to generate human disease models in vitro. We developed a genome editing system optimized for hPSCs that can be used to efficiently generate hPSC lines with targeted genetic modifications. Modified and isogenic control hPSCs are then differentiated into a relevant cell type for phenotypic characterization. We applied this approach to investigate the functional effects of human genetic variation underlying plasma lipid concentrations.

It s Complicated

It s Complicated Book
Author : Jeff Hsu
Publisher : Unknown
Release : 2013
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Coronary artery disease (CAD) and atrial fibrillation (AF) are two common complex diseases of the heart that both have strong genetic components. Although genome wide association studies in humans and genetic mapping studies in mouse models have identified genomic loci associated with these diseases, the functional genetic variants and the mechanisms by which they alter disease susceptibility are largely unknown. In this thesis we use mouse models and human atrial tissues to perform functional genomic studies to gain insight into these disease associated loci, with the central hypothesis that many disease associated variants function by regulating the expression of nearby genes. The major methods used in these studies are transcriptome profiling by expression microarrays and RNA sequencing followed by rigorous statistical analysis. Transcript expression can be considered as an intermediate phenotype to disease susceptibility; and, the identification of genetic variants that alter transcript expression may lead to the mechanism of disease association. In the first study we performed an intercross between AKR and DBA/2 inbred mouse lines to identify and replicate atherosclerosis modifier genes. Quantitative trait loci (QTL) for atherosclerosis on chromosome 2, 15, and 17 were replicated between studies. We quantified whole-genome expression of bone marrow derived macrophages and endothelial cells and found that much of the gene expression was under genetic control, including at the atherosclerosis QTLs, and that this was genetic regulation of gene expression was reproducible between experiments. In the second study we identified genes that are regulated differentially in macrophages from these two mouse strains upon cholesterol loading. We found that the atherosclerosis resistant strain had much higher up regulation of genes in the lysosomal pathway. Combining these two studies, we identified several atherosclerosis candidate modifier genes, such as \textit{Sys1}. In the third study we quantified the differences in gene expression between human left and right atria using RNA sequencing and found many genes differentially expressed at loci associated with AF in genome-wide association studies. In addition we identified a long non-coding RNA adjacent to \textit{PITX2}, at the locus with that has the highest association with AF. In the future these loci can be studied further using reporter assays to identify the causal variant as well as using genomic editing techniques to study the gene regulatory mechanism.

Translational Cardiometabolic Genomic Medicine

Translational Cardiometabolic Genomic Medicine Book
Author : Annabelle Rodriguez-Oquendo
Publisher : Academic Press
Release : 2015-09-07
ISBN : 0128004746
Language : En, Es, Fr & De

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Book Description :

Translational Cardiometabolic Genomic Medicine, edited by Dr. Annabelle Rodriguez-Oquendo, is an important resource to postgraduate (medical, dental and graduate) students, postdoctoral fellows, basic scientists, and physician scientists seeking to understand and expand their knowledge base in the field of genomic medicine as it is applied to cardiometabolic diseases. This handbook integrates cutting-edge experimental approaches such as chromatin immunoprecipitation paired end tagging (CHIA-PET), to population studies such as the Multi-Ethnic Study of Atherosclerosis. It encompasses a range of book chapters that highlight bioinformatic approaches to better understanding functionality of the noncoding regions of the human genome to the use of molecular diagnostic testing in predicting increased risk of cardiovascular diseases. Where applicable, this reference also includes chapters related to therapeutic options specifically aligned to molecular targets. Provides comprehensive research on translational genomic medicine Explains state-of-the-art genome editing for stem cells and mouse models with significant relevance to human cardiometabolic disease Includes discussions on the functional effects of single nucleotide polymorphisms and cardiometabolic diseases, stratified by sex and race Encompasses a range of book chapters that highlight bioinformatic approaches to better understanding functionality of the noncoding regions of the human genome

Genome Editing and Engineering

Genome Editing and Engineering Book
Author : Krishnarao Appasani
Publisher : Cambridge University Press
Release : 2018-07-31
ISBN : 1107170370
Language : En, Es, Fr & De

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Book Description :

A complete guide to endonuclease-based genomic engineering, from basic science to application in disease biology and clinical treatment.

Congestive Heart Failure and Cardiac Transplantation

Congestive Heart Failure and Cardiac Transplantation Book
Author : Daniel J. Garry,Robert F. Wilson,Zeev Vlodaver
Publisher : Springer
Release : 2017-06-01
ISBN : 3319445774
Language : En, Es, Fr & De

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Book Description :

This book is a comprehensive overview of heart failure and cardiac transplantation and integrates scientific and clinical information about the physiology, pathophysiology, diagnosis, and treatment of this disorder. Organized into five parts, it reviews the history and basic mechanisms of heart failure; etiology of heart failure; heart failure disease progression; advanced therapies for heart failure; and cardiac transplantation. The book presents basic concepts in the physiology, molecular biology, pathology, and epidemiology of the normal and failing heart; known causes of heart failure, such as right heart failure, valvular cardiomyopathy, molecular mechanisms of sarcomeric cardiomyopathies, and neuromuscular cardiomyopathy; cardiorenal syndrome; neurohormonal activation; cardiac resynchronization, ventricular assist devices; regenerative mechanisms; orthotopic heart transplantation; early and late management of the post-transplant patient; heart transplantation and antibody-mediated rejections; heart-lung transplantation; and cardiac xenotransplantation. Featuring contributions from leaders in the fields of heart failure, cardiac transplantation, cardiac pathology, and cardiovascular molecular research, Congestive Heart Failure and Cardiac Transplantation is a valuable compendium for cardiologists, cardiothoracic surgeons, researchers, trainees, and students.

Genetics of Hypertension

Genetics of Hypertension Book
Author : Xi Cheng,Bina Joe
Publisher : Biota Publishing
Release : 2015-08-01
ISBN : 161504695X
Language : En, Es, Fr & De

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Book Description :

Hypertension, or elevated blood pressure, is a major risk factor for various cardiovascular, renal diseases, and stroke. The form of hypertension with no identifiable cause is referred to as Essential Hypertension. Familial studies indicate that Essential Hypertension is heritable and, thereby, classical genetic approaches have been applied on both human and other mammalian models of hypertension to map the locations of the allelic variants within quantitative trait loci for blood pressure. The post genome era has further elevated this area of research into large-scale genome-wide association studies of hypertension in humans. Collectively, these studies have resulted in the prioritization and cataloging of several genomic regions containing allelic variants as candidates linked or associated with essential hypertension. Further, they are providing evidence to suggest that the inheritance of hypertension is rather complex, encompassing multiple variants both within protein-coding and non-coding annotations, each of which may act independently or interactively with other genes and/or environmental factors to differentially regulate blood pressure. This book provides an overview of the various methods employed to study the genetics of hypertension and discuss the progress and prospects of this area of research that may contribute towards individualized clinical management of hypertension in the future.

Clinical and Molecular Aspects of Cardiomyopathies On the road from gene to therapy An Issue of Heart Failure Clinics E Book

Clinical and Molecular Aspects of Cardiomyopathies  On the road from gene to therapy  An Issue of Heart Failure Clinics  E Book Book
Author : Giuseppe Limongelli
Publisher : Elsevier Health Sciences
Release : 2018-04-02
ISBN : 0323610471
Language : En, Es, Fr & De

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Book Description :

This issue of Heart Failure Clinics--edited by Drs. Giuseppe Limongelli, Sharlene Day, and Perry Elliott--will cover Clinical and Molecular Aspects of Cardiomyopathies: On the Road from Gene to Therapy. Topics include, but are not limited to, Epidemiology and Clinical aspects of Genetic Cardiomyopathies, Existing and New therapies, Gene editing and gene-based therapeutics, Controversies surrounding exercise in genetic cardiomyopathies, Genetic infiltrative cardiomyopathies, LV Noncompaction, Clinical presentation and natural history of hypertrophic cardiomyopathy in Rasopathies, and Moelcular basis and new treatments of cardiac diseases in Rasopathies.

Genome Editing and Engineering

Genome Editing and Engineering Book
Author : Krishnarao Appasani
Publisher : Cambridge University Press
Release : 2018-06-30
ISBN : 1316762084
Language : En, Es, Fr & De

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Book Description :

Recent advances in genome editing tools using endonucleases such as TALENs, ZFNs, and CRISPRs, combined with genomic engineering technologies, have opened up a wide range of opportunities from applications in the basic sciences and disease biology research, to the potential for clinical applications and the development of new diagnostic tools. This complete guide to endonuclease-based genomic engineering gives readers a thorough understanding of this rapidly expanding field. Chapters cover the discovery, basic science, and application of these techniques, focusing particularly on their potential relevance to the treatment of cancer, and cardiovascular and immunological disease. The final section discusses the legal and ethical issues which accompany the technology. Providing authoritative coverage of the potential that genome editing and engineering have, this is an ideal reference for researchers and graduate students and those working in the biotechnology and pharmaceutical industries, as well as in a clinical setting.

Research on Cardiac Differentiation from Human Pluripotent Stem Cells

Research on Cardiac Differentiation from Human Pluripotent Stem Cells Book
Author : Lorena de Oñate Monje
Publisher : Unknown
Release : 2015
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Probably, the gain in organ complexity and cell function has led to a decrease in healing capacities in the adult mammalian heart. In an effort to generate new venues for the generation of functional cardiac cells, in the present work we have explored the possibility to manipulate cell fate and plasticity making use of different technologies. First, and taking advantage of somatic cell reprogramming we have generated induced pluripotent stem cells (iPSCs) from umbilical cord derived mesenchymal stem cells (ucMSCs), an amenable source of stem cells in the clinical setting. In a very simple manner we have converted ucMSCs into iPSCs with either four transcription factors (OCT4, SOX2, KLF4 and c-MYC) or two transcription factors (OCT4 and SOX2). Second, by a cell conversion approach, we have been able to produce cells expressing cardiac-related markers at the protein level from human post-natal dermal fibroblasts. Our protocol induces cell de-differentiation by the overexpression of OCT4 and SOX2 pluripotent-related transcription factors first, and the specific meso-cardiac transcriptional factor GATA4 afterwards. Finally, in order to define new conditions for the generation of cardiac-like cells from either pluripotent stem cells or somatic cells, we have developed a reporter cell line for the cardiac gene alpha Myosin Heavy Chain (MYH6) by means of TALEN and CRISPR/CAS9 genome editing technologies. In this manner we have been able to explore culture conditions promoting cardiac differentiation from human embryonic stem cells. The work presented here shows different approaches aiming to generate cardiac-like cells with an impact in Regenerative Medicine.

Establishment of the Human Cardiac Models Using Gene Editing and Reprogramming in Human Pluripotent Stem Cells to Understand the Putative Functions of the G protein Coupled Receptor Kinase 5 Polymorphism GRK5 L41

Establishment of the Human Cardiac Models Using Gene Editing and Reprogramming in Human Pluripotent Stem Cells to Understand the Putative Functions of the G protein Coupled Receptor Kinase 5 Polymorphism  GRK5 L41   Book
Author : Minh Duc Hoang
Publisher : Unknown
Release : 2017
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Establishment of the Human Cardiac Models Using Gene Editing and Reprogramming in Human Pluripotent Stem Cells to Understand the Putative Functions of the G protein Coupled Receptor Kinase 5 Polymorphism GRK5 L41 book written by Minh Duc Hoang, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Engineering and Application of Pluripotent Stem Cells

Engineering and Application of Pluripotent Stem Cells Book
Author : Ulrich Martin,Robert Zweigerdt,Ina Gruh
Publisher : Springer
Release : 2018-02-28
ISBN : 3319735918
Language : En, Es, Fr & De

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Book Description :

​This book reviews the latest biotechnological advances with pluripotent stem cells, exploring their application in tissue engineering and medicinal chemistry. Chapters from expert contributors cover topics such as the production of transgene-free induced pluripotent stem cells (iPSCs), expansion, controlled differentiation and programming of pluripotent stem cells, and their genetic instability. Particular attention is given to the application of the pluripotent stem cells for vascularision of engineered tissue and for drug screening. This book will appeal to researchers working in regenerative medicine and drug discovery, and to bioengineers and professionals interested in stem cell research.

Cardiovascular Engineering A Protective Approach

Cardiovascular Engineering  A Protective Approach Book
Author : Shu Liu
Publisher : McGraw Hill Professional
Release : 2020-06-12
ISBN : 1260457656
Language : En, Es, Fr & De

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Book Description :

Publisher's Note: Products purchased from Third Party sellers are not guaranteed by the publisher for quality, authenticity, or access to any online entitlements included with the product. Cardiovascular engineering principles and practices with a focus on disease prevention Aimed at advanced students taking cardiovascular, regenerative, and tissue engineering courses, Cardiovascular Engineering: A Protective Approach explores applications of protective engineering strategies and technologies to common cardiovascular disorders. A protective approach to cardiovascular engineering involves studying the naturally occurring mechanisms that protect molecules, cells, and organs from injury and dysfunction. The goal is to use this understanding to design and develop engineering-based therapeutic strategies and technologies that prevent cardiovascular injury and disease. Readers will learn the fundamental and applied aspects of cardiovascular engineering. Coverage includes: • Foundations of cardiovascular protective engineering • Development of the heart, blood vessels, and blood cells • Stem cells and regeneration • Structure and function of the heart and blood vessels • Cytokines and growth factors in cardiovascular disease • Mechanisms of disease • Systems protective mechanisms against injury • Protective engineering strategies • Systemic hypertension • Atherosclerosis and arterial aneurysms • Ischemic heart disease and ischemic stroke • Cardiomyopathies and congenital heart disease

Textbook of Gene Therapy

Textbook of Gene Therapy Book
Author : Kewal K. Jain
Publisher : Seattle ; Toronto : Hogrefe & Huber
Release : 1998
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

During the past eight years following initial gene therapy experiments, more than 200 clinical protocols have been submitted worldwide and more than 2000 patients have been treated. Although a lot remains to be accomplished before gene therapy becomes a standard medical practice, enough information has accumulated to justify a textbook on the subject. Some of the procedures and products are expected to enter the marketplace and medical practice by the year 2000. The public is very much aware of the potential of gene therapy and the medical profession should prepare itself for the new developments by learning about the basics and applications of these techniques. Much of there-search has been conducted in the bio-pharmaceutical industry by gene therapy companies and the commercial opportunities for gene therapy are enormous. A large segment of the society thus has an interest in gene therapy. This book presents a unique critical review of the tremendous progress which has been made in this field. The textbook is an effort to bring a wide range of important developments together in an accessible format. This book is aimed at physicians, gene therapists, molecular biologists, nurse practitioners and students in these fields, as well as other healthcare professionals interested in developments in the field of gene therapy and its impact, both short- and long-term, on the practice of medicine. Industrial executives planning long-term strategies in gene therapy will find this handy textbook to be a comprehensive source of information on the subject and the companies involved in developing it. Prof. K. K. Jain is a neurosurgeon trained in Canada and the United States. He has heldacademic positions in major medical centers around the world. For the last decade, he has been a consultant to a number of major biopharmaceutical companies and now runs his own biotechnology company specializing in gene therapy research and documentation.

Application of Genetics and Genomics in Poultry Science

Application of Genetics and Genomics in Poultry Science Book
Author : Xiaojun Liu
Publisher : BoD – Books on Demand
Release : 2018-09-05
ISBN : 1789236304
Language : En, Es, Fr & De

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Book Description :

Genetics and genomics in poultry have been the most rapidly advancing subjects since the completion of the chicken genome sequence in 2004 and have been extensively used to understand the genetic determinants of complex traits. This book intends to provide readers with a comprehensive overview of the current progress in the application of genetic and genomic science in the poultry field. The contents cover genetic variation detection, selection methods for breeding, transgenesis and genome editing, genetic basis of disease resistance, control of gene expression and regulation, reproduction and meat quality, etc. The book should prove useful to researchers and students working in related fields.

Progress in Molecular Biology and Translational Science

Progress in Molecular Biology and Translational Science Book
Author : David B. Teplow
Publisher : Academic Press
Release : 2018-10-16
ISBN : 0128162368
Language : En, Es, Fr & De

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Book Description :

Progress in Molecular Biology and Translational Science, Volume 159, provides the most topical, informative and exciting monographs available on a wide variety of research topics related to prions, viruses, bacteria and eukaryotes. The series includes in-depth knowledge on molecular biological aspects of organismal physiology, along with insights on how this knowledge may be applied to understand and ameliorate human disease. New chapters in this release discuss timely topics, such as Targeting recently deorphanized GPR83 for the treatment of infection, stress, and drug addiction, Arrestin Structure-Function, Arrestins in the Cardiovascular System, Analysis of biased agonism, and more. Includes comprehensive coverage of molecular biology Presents ample use of tables, diagrams, schemata, and color figures to enhance the reader's ability to rapidly grasp the information provided Contains contributions from renowned experts in the field

Proceedings of 7th International Conference and Exhibition on Cell and Gene Therapy 2018

Proceedings of 7th International Conference and Exhibition on Cell and Gene Therapy 2018 Book
Author : ConferenceSeries
Publisher : ConferenceSeries
Release : 2021-06-13
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

March 15-16, 2018 London,UK Key Topics : Stem Cell Therapies, Cell Culture and Bioprocessing, Viral Gene Therapy, Gene and Cell Therapy for Rare & Common Diseases, Tissue Science & Regenerative Medicine, Molecular Basis of Epigenetics, Clinical Trials on Cell & Gene Therapy, Cell Science and Stem Cell Research, Bioengineering Therapeutics, Nano Therapy, Gene Editing Technology, Advanced Gene Therapeutics, Genetics & Genomic Medicine, Ethical Issues in Cell and Gene Therapy, Markets & Future Prospects for Cell & Gene Therapy, Cell Therapy, Gene Therapy, Cell Therapy of Cardiovascular Disorders, Cell Therapy for Neurological Disorders, Regulatory and Safety Aspects of Cell and Gene Therapy, Commercialization,