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Carbohydrate Active Enzymes

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Carbohydrate Active Enzymes

Carbohydrate Active Enzymes Book
Author : Stefano Benini
Publisher : MDPI
Release : 2020-06-18
ISBN : 3039360906
Language : En, Es, Fr & De

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Book Description :

Carbohydrate-active enzymes are responsible for both biosynthesis and the breakdown of carbohydrates and glycoconjugates. They are involved in many metabolic pathways; in the biosynthesis and degradation of various biomolecules, such as bacterial exopolysaccharides, starch, cellulose and lignin; and in the glycosylation of proteins and lipids. Carbohydrate-active enzymes are classified into glycoside hydrolases, glycosyltransferases, polysaccharide lyases, carbohydrate esterases, and enzymes with auxiliary activities (CAZy database, www.cazy.org). Glycosyltransferases synthesize a huge variety of complex carbohydrates with different degrees of polymerization, moieties and branching. On the other hand, complex carbohydrate breakdown is carried out by glycoside hydrolases, polysaccharide lyases and carbohydrate esterases. Their interesting reactions have attracted the attention of researchers across scientific fields, ranging from basic research to biotechnology. Interest in carbohydrate-active enzymes is due not only to their ability to build and degrade biopolymers—which is highly relevant in biotechnology—but also because they are involved in bacterial biofilm formation, and in glycosylation of proteins and lipids, with important health implications. This book gathers new research results and reviews to broaden our understanding of carbohydrate-active enzymes, their mutants and their reaction products at the molecular level.

Carbohydrate Active Enzymes

Carbohydrate Active Enzymes Book
Author : K.-H. Park
Publisher : Elsevier
Release : 2008-09-23
ISBN : 1845695755
Language : En, Es, Fr & De

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Book Description :

Recent advances in biochemistry and biotechnology have enabled significant progress in basic research on carbohydrate-active enzymes and advances in their effective application. The mechanism of catalytic reaction of carbohydrate-active enzymes is not fully understood, though, as they often show unusual substrate specificity and modes of action. This comprehensive collection summarises some of the most important research in the field of carbohydrate-active enzymes, focusing on the enzymatic reaction mechanism, structure-function relationship and role in the living organism The book is based on papers presented in the 2008 Agricultural Biotechnology Symposium Carbohydrate-active enzymes: structure, function and applications held on September 26th-27th 2008 in Seoul National University, Korea. This symposium was organized by the Center for Agricultural Biomaterials, Seoul National University, Korea, which has organized symposia on agricultural biotechnology annually since 1990. Many important results on new types of carbohydrate-active enzymes and their applications have been reported at these meetings. Papers in Part one of this collection focus on structure-function relationships of carbohydrate-active enzymes. Papers in Part two discuss functions and applications of carbohydrate-active enzymes, such as enzymes for grain processing and glycosidases and their mutants as useful tools for glycoside synthesis. With its distinguished editor and international team of contributors, Carbohydrate-active enzymes: structure, function and applications is an essential reference for research scientists, post-graduate students and those in the food industry with an interest in enzymes. Summarises some of the most important research in the field of carbohydrate-active enzymes Covers topics ranging from enzyme classification and structural elucidation to applications of enzymes in food processing and other industries

Carbohydrate active Enzymes Metabolising Maltose

Carbohydrate active Enzymes Metabolising Maltose Book
Author : Julia Smirnova
Publisher : Unknown
Release : 2012
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Carbohydrate active Enzymes Metabolising Maltose book written by Julia Smirnova, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Discovery and Characterization of Novel Carbohydrate active Enzymes from Marine Bacteria

Discovery and Characterization of Novel Carbohydrate active Enzymes from Marine Bacteria Book
Author : Lukas Reisky
Publisher : Unknown
Release : 2019
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Half of the global primary production is realized in the ocean and a large proportion of the fixed CO2 is used to produce algal polysaccharides. These carbohydrates are a main carbon and energy source fueling marine food webs where they are degraded into monomeric sugars by carbohydrate active enzymes. As the knowledge about the enzymatic degradation of algal polysaccharides in the ocean is still scarce, this thesis aimed to contribute to a deeper understanding of these processes. O-methylation of sugars is a common and very stable modification in marine and terrestrial polysaccharides. It was shown in Article I that cytochrome P450 monooxygenases are an integral part of agar depolymerization systems from marine bacteria where these enzymes catalyze the oxidative demethylation of 6-O-methyl-D-galactose, a monomer found in red algal polysaccharides. These findings place the P450 subfamily CYP236A into the group of carbohydrate-active enzymes as a second class of monooxygenases. The characterized P450s are highly specific for 6 O methyl D galactose and do not accept typical P450 substrates. To understand the molecular determinants for the specific conversion of the polar substrate, protein crystallography was used (Article II). The crystal structure of the P450 from Z. galactanivorans bound to a substrate molecule showed that hydrogen bonding and hydrophobic interactions are involved in substrate recognition, which was further supported by ITC experiments and mutational studies ...

Structural Studies of Carbohydrate Active Enzymes

Structural Studies of Carbohydrate Active Enzymes Book
Author : Carlos Martinez Fleites
Publisher : Unknown
Release : 2006
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Structural Studies of Carbohydrate Active Enzymes book written by Carlos Martinez Fleites, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

New approaches in starch science and carbohydrate active enzymes

New approaches in starch science and carbohydrate active enzymes Book
Author : Nihon Ōyō Toshitsu Kagakkai
Publisher : Unknown
Release : 2003
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download New approaches in starch science and carbohydrate active enzymes book written by Nihon Ōyō Toshitsu Kagakkai, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Computational Methods in the Study of Carbohydrates and Carbohydrate active Enzymes

Computational Methods in the Study of Carbohydrates and Carbohydrate active Enzymes Book
Author : Anthony David Hill
Publisher : Unknown
Release : 2006
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Computational Methods in the Study of Carbohydrates and Carbohydrate active Enzymes book written by Anthony David Hill, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Functional Characterization of Carbohydrate active Enzymes from Marine Bacteria

Functional Characterization of Carbohydrate active Enzymes from Marine Bacteria Book
Author : Marcus Bäumgen
Publisher : Unknown
Release : 2020
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Functional Characterization of Carbohydrate active Enzymes from Marine Bacteria book written by Marcus Bäumgen, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Heterologous Expression Characterization and Applications of Carbohydrate Active Enzymes and Binding Modules

Heterologous Expression  Characterization and Applications of Carbohydrate Active Enzymes and Binding Modules Book
Author : Åsa Kallas,Kungliga tekniska högskolan. Institutionen för bioteknologi
Publisher : Unknown
Release : 2006
ISBN : 9789171783493
Language : En, Es, Fr & De

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Book Description :

Download Heterologous Expression Characterization and Applications of Carbohydrate Active Enzymes and Binding Modules book written by Åsa Kallas,Kungliga tekniska högskolan. Institutionen för bioteknologi, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Plant Proteinaceous Inhibitors of Carbohydrate active Enzymes

Plant Proteinaceous Inhibitors of Carbohydrate active Enzymes Book
Author : Nathalie Juge
Publisher : Unknown
Release : 2004
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Plant Proteinaceous Inhibitors of Carbohydrate active Enzymes book written by Nathalie Juge, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Carbohydrate active Enzymes that Modify the Cell Wall of Aspergillus Niger

Carbohydrate active Enzymes that Modify the Cell Wall of Aspergillus Niger Book
Author : Marc Jos Elise Cornelis Maarel,Rijksuniversiteit Groningen
Publisher : Unknown
Release : 2014
ISBN : 9789036772372
Language : En, Es, Fr & De

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Book Description :

Download Carbohydrate active Enzymes that Modify the Cell Wall of Aspergillus Niger book written by Marc Jos Elise Cornelis Maarel,Rijksuniversiteit Groningen, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Biochemical and Structural Characterization of Two Thermostable Carbohydrate active Enzymes with Potential in Biotechnological Applications

Biochemical and Structural Characterization of Two Thermostable Carbohydrate active Enzymes with Potential in Biotechnological Applications Book
Author : Anonim
Publisher : Unknown
Release : 2014
ISBN : 9789175950945
Language : En, Es, Fr & De

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Book Description :

Download Biochemical and Structural Characterization of Two Thermostable Carbohydrate active Enzymes with Potential in Biotechnological Applications book written by , available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Carbohydrate Bioengineering

Carbohydrate Bioengineering Book
Author : Tuula T Teeri,B Svensson,H J Gilbert,T Feizi
Publisher : Royal Society of Chemistry
Release : 2007-10-31
ISBN : 1847550320
Language : En, Es, Fr & De

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Book Description :

Carbohydrate bioengineering is a rapidly expanding field with many applications in medicine and industry. Presenting state-of-the-art research, Carbohydrate Bioengineering: Interdisciplinary Approaches brings together international experts on many different aspects of this burgeoning topic. Coverage includes: the engineering of glycosidases for constructive purposes; structure-function studies and protein engineering of carbohydrate-active enzymes; chemo-enzymatic carbohydrate synthesis; and trends emerging from comprehensive work on genomes and glycomes. This timely publication will be welcomed by all those needing access to the latest research in the field, including practitioners in the medicinal, chemical, food and pharmaceutical areas.

Modification of Carbohydrate Active Enzymes in Switchgrass Panicum Virgatum L to Improve Saccharification and Biomass Yields for Biofuels

Modification of Carbohydrate Active Enzymes in Switchgrass  Panicum Virgatum L   to Improve Saccharification and Biomass Yields for Biofuels Book
Author : Jonathan Duran Willis
Publisher : Unknown
Release : 2016
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

The natural recalcitrance of plant cell walls is a major commercial hurdle for plant biomass to be converted into a viable energy source as alternative to fossil fuels. To circumvent this hurdle manipulation of carbohydrate enzymes active in the cellulose and hemicellulose portions of the plant cell wall can be utilized to improve feedstocks. Production of cellulolytic enzymes by plants have been evaluated for reducing the cost associated with lignocellulosic biofuels. Plants have successfully served as bioreactors producing bacterial and fungal glycosyl hydrolases, which have altered plant growth to improve saccharification. A bioprospecting opportunity lies with the utilization of insect glycosyl hydrolases for transgenic production in plants. Lessons learned from microbial hydrolase expression can be applied to insect hydrolase expression along with gene stacking to develop autohydrolysis plant lines. A step toward production of insect cellulases in plants was performed by insertion of the endoglucanase TcEG1 gene, from Tribolium castaneum, into switchgrass. Transgenic lines overexpressing TcEG1 produced a functional enzyme with an optimal alkaline pH activity of 12.0. Recalcitrance was assayed by performing saccharification analysis, in which one line was superior over non-transgenic control; this line also had reduced 9% lignin content. Transgenic lines developed narrow stems, although biomass yield was unchanged due to increased tiller number and cell wall thickness. Grasses contain a relatively high amount of glucoarabinoxylan in their cell walls, which cross links with lignin. By down-regulation of a uridine diphosphate arabinomutase (UAM) gene via RNAi, it was hypothesized that attenuated production of this carbohydrate transferase would increase saccharification of switchgrass biomass from a disruption of cross linking. Transgenic events showed a reduction in arabinose content (up to 58%) and altered arabino-side chains, however saccharification was unchanged. UAM transgenic switchgrass showed a red node phenotype, which could be in response to increased lignin biosynthesis. A model of UAMinfluenced cell wall interactions was proposed and will used to build hypotheses for future - omics research. In summary, switchgrass saccharification and biomass yield can be increased by introduction of carbohydrate active enzymes. Combination of presented transgenic lines with low-lignin germplasm could be utilized to further improve saccharification yield.