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3D Bioprinting

3D Bioprinting Book
Author : Jeremy M. Crook
Publisher : Humana
Release : 2021-03-24
ISBN : 9781071605226
Language : En, Es, Fr & De

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Book Description :

This volume explores the latest developments and contributions to the field of 3D bioprinting, and discusses its use for quality R&D and translation. The chapters in this book are divided into two parts: Part one covers generic themes in bioprinting to introduce novice readers to the field, while also providing experts with new and helpful information. Part two discusses protocols used to prepare, characterize, and print a variety of biomaterials, cells, and tissues. These chapters also emphasize methods used for printing defined and humanized constructs suitable for human tissue modelling in research and applicable to clinical product development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, 3D Bioprinting: Methods and Protocols is a valuable resource for researchers and bioprinting laboratories/facilities interested in learning more about this rapidly evolving technology.

3D Bioprinting

3D Bioprinting Book
Author : Ibrahim Tarik Ozbolat
Publisher : Academic Press
Release : 2016-11-21
ISBN : 0128030305
Language : En, Es, Fr & De

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Book Description :

3D Bioprinting: Fundamentals, Principles and Applications provides the latest information on the fundamentals, principles, physics, and applications of 3D bioprinting. It contains descriptions of the various bioprinting processes and technologies used in additive biomanufacturing of tissue constructs, tissues, and organs using living cells. The increasing availability and decreasing costs of 3D printing technologies are driving its use to meet medical needs, and this book provides an overview of these technologies and their integration. Each chapter discusses current limitations on the relevant technology, giving future perspectives. Professor Ozbolat has pulled together expertise from the fields of bioprinting, tissue engineering, tissue fabrication, and 3D printing in his inclusive table of contents. Topics covered include raw materials, processes, machine technology, products, applications, and limitations. The information in this book will help bioengineers, tissue and manufacturing engineers, and medical doctors understand the features of each bioprinting process, as well as bioink and bioprinter types. In addition, the book presents tactics that can be used to select the appropriate process for a given application, such as tissue engineering and regenerative medicine, transplantation, clinics, or pharmaceutics. Describes all aspects of the bioprinting process, from bioink processing through design for bioprinting, bioprinting techniques, bioprinter technologies, organ printing, applications, and future trends Provides a detailed description of each bioprinting technique with an in-depth understanding of its process modeling, underlying physics and characteristics, suitable bioink and cell types printed, and major accomplishments achieved thus far Explains organ printing technology in detail with a step-by-step roadmap for the 3D bioprinting of organs from isolating stem cells to the post-transplantation of organs Presents tactics that can be used to select the appropriate process for a given application, such as tissue engineering and regenerative medicine, transplantation, clinics, or pharmaceutics

Bioprinting

Bioprinting Book
Author : Maika G. Mitchell
Publisher : Academic Press
Release : 2017-02-17
ISBN : 0128094036
Language : En, Es, Fr & De

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Book Description :

Innovation is added value to a known process. Bioprinting: Techniques and Risks for Regenerative Medicine aims to stimulate a scientifically grounded, interdisciplinary, multiscale debate and exchange of ideas using the techniques described in the book. 3D printing and additive manufacturing evolved from within the field of Cell Biology will have the ability to recreate cells queried from large amounts of phenotypic and molecular data. Stem Cell biologists, biotechnologists and material engineers, as well as graduate students will greatly benefit from the practical knowledge and case examples provided throughout this book. Shows the possible risk of rejection of 3D printed cells. Contains bioprinting techniques in literature plus actual 3D files adapted and created by the author using several types of 3d printers Provides information on how to convert an existing 3-D printer to bioprinter using currently available techniques Describes the increased complexity of bioprinting compared to 3D- printing Discussion on how 3D printing and additive manufacturing offers the opportunity to 3D print an entire organ, reducing the associated costs of this process when using cells as bioink

Bioprinting

Bioprinting Book
Author : Chee Kai Chua,Wai Yee Yeong
Publisher : World Scientific Publishing Co Inc
Release : 2014-11-27
ISBN : 9814612138
Language : En, Es, Fr & De

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Book Description :

At labs around the world, researchers have been experimenting with bioprinting, first just to see whether it was possible to push cells through a printhead without killing them (in most cases it is), and then trying to make cartilage, bone, skin, blood vessels, small bits of liver and other tissues. There are other ways to try to “engineer” tissue — one involves creating a scaffold out of plastics or other materials and adding cells to it. In theory, at least, a bioprinter has advantages in manipulating control of the placement of cells and other components to mimic natural structures. But just as the claims made for 3-D printing technology sometimes exceed the reality, the field of bioprinting has seen its share of hype. The reality is that, although bioprinting researchers have made great strides, there are many formidable obstacles to overcome. Nobody who has any credibility claims they can print organs, or believes in their heart of hearts that that will happen in the next 20 years, but for operations like hip replacement, advance in Bio-printing has made customization of certain body parts possible. This book will start from the concept of Tissue Engineering, covering various approaches in Scaffolds for tissue engineering, Bioprinting techniques and Materials for bioprinting, Cell processing, 3D cell culture techniques, Computational design and simulation, multi-disciplinary approaches in bioprinting and finally cover the applications of bioprinting.

Bioprinting

Bioprinting Book
Author : Kenneth Douglas
Publisher : Unknown
Release : 2021
ISBN : 9780190943554
Language : En, Es, Fr & De

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Book Description :

"Bioprinting: To Make Ourselves Anew describes how bioprinting emerged from 3D printing and details the accomplishments and challenges in bioprinting tissues of cartilage, skin, bone, muscle, neuromuscular junctions, liver, heart, lung, and kidney. It explains how scientists are attempting to provide these bioprinted tissues with a blood supply and the ability to carry nerve signals so that the tissues might be used for transplantation into persons with diseased or damaged organs. The book presents all the common terms in the bioprinting field and clarifies their meaning using plain language. The reader will learn about bioink-a bioprinting material containing living cells and supportive biomaterials. Additionally, readers will become at ease with concepts such as fugitive inks (sacrificial inks used to make channels for blood flow), extracellular matrices (the biological environment surrounding cells), decellularization (the process of isolating cells from their native environment), hydrogels (water-based substances that can substitute for the extracellular matrix), rheology (the flow properties of a bioink), bioreactors (containers to provide the environment cells need to thrive and multiply). Further vocabulary that will become familiar includes diffusion (passive movement of oxygen and nutrients from regions of high concentration to regions of low concentration), stem cells (cells with the potential to develop into different bodily cell types), progenitor cells (early descendants of stem cells), gene expression (the process by which proteins develop from instructions in our DNA), and growth factors (substances-often proteins-that stimulate cell growth, proliferation, and differentiation). The book contains an extensive glossary for quick reference"--

Bioprinting

Bioprinting Book
Author : Michele Conti,Michele Marino
Publisher : Academic Press
Release : 2022-05-05
ISBN : 0323854311
Language : En, Es, Fr & De

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Book Description :

Bioprinting: From Multidisciplinary Design to Emerging Opportunities describes state-of-the-art techniques and highlights open issues of different aspects that affect the efficiency of bioprinting protocols. Starting from a description of the main bioprinting technologies, the book addresses the most advanced approaches for the design of "on-demand" biomaterials suitable for incorporating biological components, along with the challenges associated with the development of a cellular model, and with the biological read-out. Coverage includes intelligent process design techniques, emerging technologies, and specific applications. Written by a highly interdisciplinary team of authors and presenting a unified approach to bioprinting, this book is ideal for doctoral and postdoctoral researchers in biotechnology, engineering, and physics, as well as industrial researchers interested in the applications of bioprinting. • Presents the basic methodological aspects in common between different applications of bioprinting • Covers the most advanced approaches, including novel technologies, printable chemical strategies for 3D biomaterials, multi-criteria bioinks evaluation, bioprinting for skeletal tissue regeneration, and disease modeling • Provides protocols, global perspectives, and up-to-date techniques by leading experts in the field

3D Bioprinting in Medicine

3D Bioprinting in Medicine Book
Author : Murat Guvendiren
Publisher : Springer
Release : 2019-08-02
ISBN : 3030239063
Language : En, Es, Fr & De

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Book Description :

This book provides current and emerging developments in bioprinting with respect to bioprinting technologies, bioinks, applications, and regulatory pathways. Topics covered include 3D bioprinting technologies, materials such as bioinks and bioink design, applications of bioprinting complex tissues, tissue and disease models, vasculature, and musculoskeletal tissue. The final chapter is devoted to clinical applications of bioprinting, including the safety, ethical, and regulatory aspects. This book serves as a go-to reference on bioprinting and is ideal for students, researchers and professionals, including those in academia, government, the medical industry, and healthcare.

Injectable Hydrogels for 3D Bioprinting

Injectable Hydrogels for 3D Bioprinting Book
Author : Insup Noh,Xiumei Wang,Sandra van Vlierberghe
Publisher : Royal Society of Chemistry
Release : 2021-07-30
ISBN : 1788018834
Language : En, Es, Fr & De

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Book Description :

Hydrogels represent one of the cornerstones in tissue engineering and regenerative medicine, due to their biocompatibility and physiologically relevant properties. These inherent characteristics mean that they can be widely exploited as bioinks in 3D bioprinting for tissue engineering applications as well as injectable gels for cell therapy and drug delivery purposes. The research in these fields is booming and this book provides the reader with a terrific introduction to the burgeoning field of injectable hydrogel design, bioprinting and tissue engineering. Edited by three leaders in the field, users of this book will learn about different classes of hydrogels, properties and synthesis strategies to produce bioinks. A section devoted to the key processing and design challenges at the hydrogel/3D bioprinting/tissue interface is also covered. The final section of the book closes with pertinent clinical applications. Tightly edited, the reader will find this book to be a coherent resource to learn from. It will appeal to those working across biomaterials science, chemical and biomedical engineering, tissue engineering and regenerative medicine.

Bioprinting

Bioprinting Book
Author : Kenneth Douglas
Publisher : Oxford University Press
Release : 2021-04-30
ISBN : 0190943564
Language : En, Es, Fr & De

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Book Description :

Of the 121,000 people on donor lists in the U.S., over 100,000 need kidney transplants and thousands die each year while waiting. Bioprinting aspires to build healthy kidney tissue from a patient's own cells and transplant this to boost failing kidneys without fear of rejection... As the 21st century dawned, a handful of inspired scientists tried to use 3D printing to create living human tissue. Their vision was to restore the health of people with intractable injuries, such as worn out cartilage, severed nerves, ailing kidneys, failing heartsthe gamut of human frailties. Their modest success energized others to join the quest. Now, after two decades of ingenious effort and hard work, they have carved out a vibrant new discipline: bioprinting. In Bioprinting: To Make Ourselves Anew, physicist Kenneth Douglas casts an eye over the achievements and future of bioprinting. He explains the science with rigor but with a minimum of technical baggage. This is the first book on the subject written expressly for the lay audience: accessible and even entertaining. Douglas interviewed two dozen bioprinting researchers from around the world, and he enriches the narrative by sharing stories from the scientists behind the science. These contemporary vignettes are complemented by historical accounts of the women and men whose prescient contributions were foundational to the development of bioprinting. The book describes the challenges and accomplishments in the bioprinting of blood vessels, cartilage, skin, bone, skeletal muscle, neuromuscular junctions, liver, heart, lung, kidney, and so-called organs-on-a-chip, as well as the challenges of providing a blood supply and nerves to bioprinted tissues. This is a compelling tale of a work in progress: to imitate nature and help heal people with debilitating afflictions.

3D Bioprinting

3D Bioprinting Book
Author : Jeremy M. Crook
Publisher : Springer Nature
Release : 2020-03-23
ISBN : 1071605208
Language : En, Es, Fr & De

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Book Description :

This volume explores the latest developments and contributions to the field of 3D bioprinting, and discusses its use for quality R&D and translation. The chapters in this book are divided into two parts: Part one covers generic themes in bioprinting to introduce novice readers to the field, while also providing experts with new and helpful information. Part two discusses protocols used to prepare, characterize, and print a variety of biomaterials, cells, and tissues. These chapters also emphasize methods used for printing defined and humanized constructs suitable for human tissue modelling in research and applicable to clinical product development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, 3D Bioprinting: Methods and Protocols is a valuable resource for researchers and bioprinting laboratories/facilities interested in learning more about this rapidly evolving technology.

3D Bioprinting in Tissue and Organ Regeneration

3D Bioprinting in Tissue and Organ Regeneration Book
Author : Yang Wu,Jerry Fuh,Ibrahim Tarik Ozbolat
Publisher : Academic Press
Release : 2022-09-10
ISBN : 0323859054
Language : En, Es, Fr & De

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Book Description :

3D Bioprinting in Tissue and Organ Regeneration covers state-of-the-art advances and applications in bioprinting. Beginning with an introduction that considers techniques, bioinks and construct design, the authors then move onto a detailed review of applications of bioprinting in different biomedical fields (skin, cartilage, bone, vascularized tissue, etc.). This is followed by a chapter overview of intraoperative bioprinting, which is widely considered one of the important future trends in this area. Finally, the authors tackle ethical and regulation concerns regarding the utilization of bioprinting. The book is written by three global experts for an audience of students and professionals with some basic knowledge of bioprinting, but who seek a deeper understanding of the biomedical applications involved in bioprinting. Introduces readers to bioprinting modalities, as well as pre-bioprinting, bioprinting and post-bioprinting procedures Focuses on biomedical applications used in bioprinting in chapters specific to skin, cartilage, bone and vascularized tissue Provides readers with original ideas from engineering and clinical points-of-view that are based on the authors’ extensive experience in this field, as well as the possibilities of future translation of bioprinting technologies from bench to bedside

Cell Assembly with 3D Bioprinting

Cell Assembly with 3D Bioprinting Book
Author : Yong He,Qing Gao,Yifei Jin
Publisher : John Wiley & Sons
Release : 2022-03-14
ISBN : 3527347968
Language : En, Es, Fr & De

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Book Description :

Provides an up-to-date outline of cell assembly methods and applications of 3D bioprinting Cell Assembly with 3D Bioprinting provides an accesible overview of the layer-by-layer manufacturing of living structures using biomaterials. Focusing on technical implemention in medical and bioengineering applications, this practical guide summarize each key aspect of the 3D bioprinting process. Contributions from a team of leading researchers describe bioink preparation, printing method selection, experimental protocols, integration with specific applications, and more. Detailed, highly illustrated chapters cover different bioprinting approaches and their applications, including coaxial bioprinting, digital light projection, direct ink writing, liquid support bath-assisted 3D printing, and microgel-, microfiber-, and microfluidics-based biofabrication. The book includes practical examples of 3D bioprinting, a protocol for typical 3D bioprinting, and relevant experimental data drawn from recent research. * Highlights the interdisciplinary nature of 3D bioprinting and its applications in biology, medicine, and pharmaceutical science * Summarizes a variety of commonly used 3D bioprinting methods * Describes the design and preparation of various types of bioinks * Discusses applications of 3D bioprinting such as organ development, toxicological research, clinical transplantation, and tissue repair Covering a wide range of topics, Cell Assembly with 3D Bioprinting is essential reading for advanced students, academic researchers, and industry professionals in fields including biomedicine, tissue engineering, bioengineering, drug development, pharmacology, bioglogical screening, and mechanical engineering.

Towards 4D Bioprinting

Towards 4D Bioprinting Book
Author : Adrian Neagu
Publisher : Academic Press
Release : 2022-12-01
ISBN : 012818745X
Language : En, Es, Fr & De

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Book Description :

Towards 4D Printing presents the current state of three-dimensional (3D) bioprinting and its recent offspring, 4D bioprinting. These are attractive approaches to tissue engineering because they hold the promise of building bulky tissue constructs with incorporated vasculature. Starting with the discussion of 3D and 4D printing of inanimate objects, the book presents several 3D bioprinting techniques and points out the challenges imposed by living cells on the bioprinting process. It argues that, in order to fine-tune the bioprinter, one needs a quantitative analysis of the conditions experienced by cells during printing. Once the printing is over, the construct evolves according to mechanisms known from developmental biology. These are described in the book along with computer simulations that aim to predict the outcome of 3D bioprinting. In addition, the book provides the latest information on the principles and applications of 4D bioprinting, such as for medical devices and assistive technology. The last chapter discusses the perspectives of the field. This book provides an up-to date description of the theoretical tools developed for the optimization of 3D bioprinting, presents the morphogenetic mechanisms responsible for the post-printing evolution of the bioprinted construct and describing computational methods for simulating this evolution, and discusses the leap from 3D to 4D bioprinting in the light of the latest developments in the field. Most importantly, Towards 4D Printing explains the importance of theoretical modeling for the progress of 3D and 4D bioprinting. Presents theoretical tools needed for the optimization of the bioprinting process Describes the principles and implementation of computer simulations needed to predict the outcome of 3D bioprinting Analyzes the distinctive features of 4D bioprinting along with its applications and perspectives

3D Bioprinting for Reconstructive Surgery

3D Bioprinting for Reconstructive Surgery Book
Author : Daniel Thomas,Zita M. Jessop,Iain S. Whitaker
Publisher : Woodhead Publishing
Release : 2017-11-14
ISBN : 0081012160
Language : En, Es, Fr & De

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Book Description :

3D Bioprinting for Reconstructive Surgery: Techniques and Applications examines the combined use of materials, procedures and tools necessary for creating structural tissue constructs for reconstructive purposes. Offering a broad analysis of the field, the first set of chapters review the range of biomaterials which can be used to create 3D-printed tissue constructs. Part Two looks at the techniques needed to prepare biomaterials and biological materials for 3D printing, while the final set of chapters examines application-specific examples of tissues formed from 3D printed biomaterials. 3D printing of biomaterials for tissue engineering applications is becoming increasingly popular due to its ability to offer unique, patient-specific parts—on demand—at a relatively low cost. This book is a valuable resource for biomaterials scientists, biomedical engineers, practitioners and students wishing to broaden their knowledge in the allied field. Discusses new possibilities in tissue engineering with 3D printing Presents a comprehensive coverage of the materials, techniques and tools needed for producing bioprinted tissues Reviews emerging technologies in addition to commercial techniques

3D Bioprinting Revolution

3D Bioprinting Revolution Book
Author : Dr. Sabrie Soloman
Publisher : KHANNA PUBLISHING HOUSE
Release : 2022-12-03
ISBN : 9389139082
Language : En, Es, Fr & De

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Book Description :

This book provide a detailed guide and optimum implementations to each of the stated 3D printing technology, the basic understanding of its operation, and the similarity as well as the dissimilarity functions of each printer. School Students, University undergraduates =, and ost graduate students will find the book if immense value to equip them not only with the fundamental in design and implementation but also will encourage them to acquire a system and practice creating their own innovative samples. Furthermore, professionals and educators will be well prepared to use the knowledge and the expertise to practice and advance the technology for the ultimate good of their respective organizations.

3D Bioprinting and Nanotechnology in Tissue Engineering and Regenerative Medicine

3D Bioprinting and Nanotechnology in Tissue Engineering and Regenerative Medicine Book
Author : Lijie Grace Zhang,Kam Leong,John P. Fisher
Publisher : Academic Press
Release : 2022-02-18
ISBN : 0128245530
Language : En, Es, Fr & De

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Book Description :

3D Bioprinting and Nanotechnology in Tissue Engineering and Regenerative Medicine, Second Edition provides an in-depth introduction to bioprinting and nanotechnology and their industrial applications. Sections cover 4D Printing Smart Multi-responsive Structure, Cells for Bioprinting, 4D Printing Biomaterials, 3D/4D printing functional biomedical devices, 3D Printing for Cardiac and Heart Regeneration, Integrating 3D printing with Ultrasound for Musculoskeletal Regeneration, 3D Printing for Liver Regeneration, 3D Printing for Cancer Studies, 4D Printing Soft Bio-robots, Clinical Translation and Future Directions. The book's team of expert contributors have pooled their expertise in order to provide a summary of the suitability, sustainability and limitations of each technique for each specific application. The increasing availability and decreasing costs of nanotechnologies and 3D printing technologies are driving their use to meet medical needs. This book provides an overview of these technologies and their integration. Includes clinical applications, regulatory hurdles, and a risk-benefit analysis of each technology Assists readers in selecting the best materials and how to identify the right parameters for printing Includes the advantages of integrating 3D printing and nanotechnology in order to improve the safety of nano-scale materials for biomedical applications

3D Bioprinting in Regenerative Engineering

3D Bioprinting in Regenerative Engineering Book
Author : Ali Khademhosseini,Gulden Camci-Unal
Publisher : CRC Press
Release : 2018-04-17
ISBN : 1315280485
Language : En, Es, Fr & De

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Book Description :

Regenerative engineering is the convergence of developmental biology, stem cell science and engineering, materials science, and clinical translation to provide tissue patches or constructs for diseased or damaged organs. Various methods have been introduced to create tissue constructs with clinically relevant dimensions. Among such methods, 3D bioprinting provides the versatility, speed and control over location and dimensions of the deposited structures. Three-dimensional bioprinting has leveraged the momentum in printing and tissue engineering technologies and has emerged as a versatile method of fabricating tissue blocks and patches. The flexibility of the system lies in the fact that numerous biomaterials encapsulated with living cells can be printed. This book contains an extensive collection of papers by world-renowned experts in 3D bioprinting. In addition to providing entry-level knowledge about bioprinting, the authors delve into the latest advances in this technology. Furthermore, details are included about the different technologies used in bioprinting. In addition to the equipment for bioprinting, the book also describes the different biomaterials and cells used in these approaches. This text: Presents the principles and applications of bioprinting Discusses bioinks for 3D printing Explores applications of extrusion bioprinting, including past, present, and future challenges Includes discussion on 4D Bioprinting in terms of mechanisms and applications

Bioprinting in Regenerative Medicine

Bioprinting in Regenerative Medicine Book
Author : Kursad Turksen
Publisher : Springer
Release : 2015-09-02
ISBN : 3319213865
Language : En, Es, Fr & De

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Book Description :

This volume presents the current state of laser-assisted bioprinting, a cutting edge tissue engineering technology. Nineteen chapters discuss the most recent developments in using this technology for engineering different types of tissue. Beginning with an overview, the discussion covers bioprinting in cell viability and pattern viability, tissue microfabrication to study cell proliferation, microenvironment for controlling stem cell fate, cell differentiation, zigzag cellular tubes, cartilage tissue engineering, osteogenesis, vessel substitutes, skin tissue and much more. Because bioprinting is on its way to becoming a dominant technology in tissue-engineering, Bioprinting in Regenerative Medicine is essential reading for those researching or working in regenerative medicine, tissue engineering or translational research. Those studying or working with stem cells who are interested in the development of the field will also find the information invaluable.

3D Bioprinting in Regenerative Engineering

3D Bioprinting in Regenerative Engineering Book
Author : Ali Khademhosseini,Gulden Camci-Unal
Publisher : CRC Press
Release : 2018-04-17
ISBN : 1315280477
Language : En, Es, Fr & De

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Book Description :

Regenerative engineering is the convergence of developmental biology, stem cell science and engineering, materials science, and clinical translation to provide tissue patches or constructs for diseased or damaged organs. Various methods have been introduced to create tissue constructs with clinically relevant dimensions. Among such methods, 3D bioprinting provides the versatility, speed and control over location and dimensions of the deposited structures. Three-dimensional bioprinting has leveraged the momentum in printing and tissue engineering technologies and has emerged as a versatile method of fabricating tissue blocks and patches. The flexibility of the system lies in the fact that numerous biomaterials encapsulated with living cells can be printed. This book contains an extensive collection of papers by world-renowned experts in 3D bioprinting. In addition to providing entry-level knowledge about bioprinting, the authors delve into the latest advances in this technology. Furthermore, details are included about the different technologies used in bioprinting. In addition to the equipment for bioprinting, the book also describes the different biomaterials and cells used in these approaches. This text: Presents the principles and applications of bioprinting Discusses bioinks for 3D printing Explores applications of extrusion bioprinting, including past, present, and future challenges Includes discussion on 4D Bioprinting in terms of mechanisms and applications

3D Bioprinting

3D Bioprinting Book
Author : Dong-Woo Cho,Byoung Soo Kim,Jinah Jang,Ge Gao,Wonil Han,Narendra K. Singh
Publisher : Springer Nature
Release : 2019-12-04
ISBN : 303032222X
Language : En, Es, Fr & De

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Book Description :

This text advances fundamental knowledge in modeling in vitro tissues/organs as an alternative to 2D cell culture and animal testing. Prior to engineering in vitro tissues/organs,the descriptions of prerequisites (from pre-processing to post-processing) in modeling in vitro tissues/organs are discussed. The most prevalent technologies that have been widely used for establishing the in vitro tissue/organ models are also described, including transwell, cell spheroids/sheets, organoids, and microfluidic-based chips. In particular, the authors focus on 3D bioprinting in vitro tissue/organ models using tissue-specific bioinks. Several representative bioprinting methods and conventional bioinks are introduced. As a bioink source, decellularized extracellular matrix (dECM) are importantly covered, including decellularization methods, evaluation methods for demonstrating successful decellularization, and material safety. Taken together, the authors delineate various application examples of 3D bioprinted in vitro tissue/organ models especially using dECM bioinks.