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Biophysical Mass Spectrometry

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Mass Spectrometry in Biophysics

Mass Spectrometry in Biophysics Book
Author : Igor A. Kaltashov,Stephen J. Eyles
Publisher : John Wiley & Sons
Release : 2005-05-06
ISBN : 0471705160
Language : En, Es, Fr & De

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Book Description :

The first systematic summary of biophysical mass spectrometrytechniques Recent advances in mass spectrometry (MS) have pushed the frontiersof analytical chemistry into the biophysical laboratory. As aresult, the biophysical community's acceptance of MS-based methods,used to study protein higher-order structure and dynamics, hasaccelerated the expansion of biophysical MS. Despite this growing trend, until now no single text has presentedthe full array of MS-based experimental techniques and strategiesfor biophysics. Mass Spectrometry in Biophysics expertly closesthis gap in the literature. Covering the theoretical background and technical aspects of eachmethod, this much-needed reference offers an unparalleled overviewof the current state of biophysical MS. Mass Spectrometry inBiophysics begins with a helpful discussion of general biophysicalconcepts and MS-related techniques. Subsequent chaptersaddress: * Modern spectrometric hardware * High-order structure and dynamics as probed by various MS-basedmethods * Techniques used to study structure and behavior of non-nativeprotein states that become populated under denaturingconditions * Kinetic aspects of protein folding and enzyme catalysis * MS-based methods used to extract quantitative information onprotein-ligand interactions * Relation of MS-based techniques to other experimental tools * Biomolecular properties in the gas phase Fully referenced and containing a helpful appendix on the physicsof electrospray mass spectrometry, Mass Spectrometry in Biophysicsalso offers a compelling look at the current challenges facingbiomolecular MS and the potential applications that will likelyshape its future.

Mass Spectrometry in Structural Biology and Biophysics

Mass Spectrometry in Structural Biology and Biophysics Book
Author : Igor A. Kaltashov,Stephen J. Eyles
Publisher : John Wiley & Sons
Release : 2012-04-03
ISBN : 0470937793
Language : En, Es, Fr & De

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Book Description :

The definitive guide to mass spectrometry techniques in biology and biophysics The use of mass spectrometry (MS) to study the architecture and dynamics of proteins is increasingly common within the biophysical community, and Mass Spectrometry in Structural Biology and Biophysics: Architecture, Dynamics, and Interaction of Biomolecules, Second Edition provides readers with detailed, systematic coverage of the current state of the art. Offering an unrivalled overview of modern MS-based armamentarium that can be used to solve the most challenging problems in biophysics, structural biology, and biopharmaceuticals, the book is a practical guide to understanding the role of MS techniques in biophysical research. Designed to meet the needs of both academic and industrial researchers, it makes mass spectrometry accessible to professionals in a range of fields, including biopharmaceuticals. This new edition has been significantly expanded and updated to include the most recent experimental methodologies and techniques, MS applications in biophysics and structural biology, methods for studying higher order structure and dynamics of proteins, an examination of other biopolymers and synthetic polymers, such as nucleic acids and oligosaccharides, and much more. Featuring high-quality illustrations that illuminate the concepts described in the text, as well as extensive references that enable the reader to pursue further study, Mass Spectrometry in Structural Biology and Biophysics is an indispensable resource for researchers and graduate students working in biophysics, structural biology, protein chemistry, and related fields.

Biophysical Characterization of Proteins in Developing Biopharmaceuticals

Biophysical Characterization of Proteins in Developing Biopharmaceuticals Book
Author : Damian J. Houde,Steven A. Berkowitz
Publisher : Elsevier
Release : 2019-11-13
ISBN : 0444641742
Language : En, Es, Fr & De

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Book Description :

Biophysical Characterization of Proteins in Developing Biopharmaceuticals, Second Edition, presents the latest on the analysis and characterization of the higher-order structure (HOS) or conformation of protein based drugs. Starting from the very basics of protein structure, this book explains the best way to achieve this goal using key methods commonly employed in the biopharmaceutical industry. This book will help today’s industrial scientists plan a career in this industry and successfully implement these biophysical methodologies. This updated edition has been fully revised, with new chapters focusing on the use of chromatography and electrophoresis and the biophysical characterization of very large biopharmaceuticals. In addition, best practices of applying statistical analysis to biophysical characterization data is included, along with practical issues associated with the concept of a biopharmaceutical’s developability and the technical decision-making process needed when dealing with biophysical characterization data. Presents basic protein characterization methods and tools applicable to (bio)pharmaceutical research and development Highlights the capabilities and limitations of each technique Discusses the underlining science of each tool Empowers industrial biophysical chemists by providing a roadmap for applying biophysical tools Outlines the needs for new characterization and analytical tools in the biopharmaceutical industry

Biophysical Techniques in Photosynthesis

Biophysical Techniques in Photosynthesis Book
Author : Thijs Aartsma,Jörg Matysik
Publisher : Springer Science & Business Media
Release : 2008-02-01
ISBN : 1402082509
Language : En, Es, Fr & De

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Book Description :

Since the first volume on Biophysical Techniques in Photosynthesis Research, published in 1996, new experimental techniques and methods have been devised at a rapid pace. The present book is a sequel which complements the publication of the first volume by providing a comprehensive overview of the most important new techniques developed over the past ten years, especially those that are relevant for research on the mechanism and fundamental aspects of photosynthesis.

Methods in Modern Biophysics

Methods in Modern Biophysics Book
Author : Bengt Nölting
Publisher : Springer Science & Business Media
Release : 2013-03-09
ISBN : 3662053675
Language : En, Es, Fr & De

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Book Description :

Incorporating dramatic recent advances, "Methods in Modern Biophysics" presents a fresh and timely introduction to modern biophysical methods. This innovative text surveys and explains the ten key biophysical methods, including those related to biophysical nanotechnology, scanning probe microscopy, X-ray crystallography, ion mobility spectrometry, mass spectrometry, and proteomics. Containing much information previously unavailable in tutorial form, "Methods in Modern Biophysics" employs worked examples and more than 260 illustrations to fully detail the techniques and their underlying mechanisms. The book was written for advanced undergraduate and graduate students, postdocs, researchers, lecturers and professors in biophysics, biochemistry, general biology and related fields.

Introduction to Biophysical Methods for Protein and Nucleic Acid Research

Introduction to Biophysical Methods for Protein and Nucleic Acid Research Book
Author : Jay A. Glasel,Murray P. Deutscher
Publisher : Academic Press
Release : 1995-11-20
ISBN : 9780080534985
Language : En, Es, Fr & De

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Book Description :

The first of its kind, Introduction to Biophysical Methods for Protein and Nucleic Acid Research serves as a text for the experienced researcher and student requiring an introduction to the field. Each chapter presents a description of the physical basis of the method, the type of information that may be obtained with the method, how data should be analyzed and interpreted and, where appropriate, practical tips about procedures and equipment. Key Features * Modern Use of Mass Spectroscopy * NMR Spectroscopy * Molecular Modeling and Graphics * Macintosh and DOS/Windows 3.x disks

Biophysical Techniques in Drug Discovery

Biophysical Techniques in Drug Discovery Book
Author : Angeles Canales
Publisher : Royal Society of Chemistry
Release : 2017-11-14
ISBN : 1788012860
Language : En, Es, Fr & De

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Book Description :

Biophysical techniques are used in many key stages of the drug discovery process including in screening for new receptor ligands, in characterising drug mechanisms, and in validating data from biochemical and cellular assays. This book provides an overview of the biophysical methods applied in drug discovery today, including traditional techniques and newer developments. Perspectives from academia and industry across a spectrum of techniques are brought together in a single volume. Small and biotherapeutic approaches are covered and strengths and limitations of each technique are presented. Case studies illustrate the application of each technique in real applied examples. Finally, the book covers recent developments in areas such as electron microscopy with discussions of their possible impact on future drug discovery. This is a go-to volume for biophysicists, analytical chemists and medicinal chemists providing a broad overview of techniques of contemporary interest in drug discovery.

Biophysics

Biophysics Book
Author : Mark C. Leake
Publisher : CRC Press
Release : 2016-09-15
ISBN : 1498702511
Language : En, Es, Fr & De

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Book Description :

An Up-to-Date Toolbox for Probing Biology Biophysics: Tools and Techniques covers the experimental and theoretical tools and techniques of biophysics. It addresses the purpose, science, and application of all physical science instrumentation and analysis methods used in current research labs. The book first presents the historical background, concepts, and motivation for using a physical science toolbox to understand biology. It then familiarizes students from the physical sciences with essential biological knowledge. The text subsequently focuses on experimental biophysical techniques that primarily detect biological components or measure/control biological forces. The author describes the science and application of key tools used in imaging, detection, general quantitation, and biomolecular interaction studies, which span multiple length and time scales of biological processes both in the test tube and in the living organism. Moving on to theoretical biophysics tools, the book presents computational and analytical mathematical methods for tackling challenging biological questions. It concludes with a discussion of the future of this exciting field. Future innovators will need to be trained in multidisciplinary science to be successful in industry, academia, and government support agencies. Addressing this challenge, this textbook educates future leaders on the development and application of novel physical science approaches to solve complex problems linked to biological questions.

Methods in Molecular Biophysics

Methods in Molecular Biophysics Book
Author : Igor N. Serdyuk,Nathan R. Zaccai,Joseph Zaccai,Giuseppe Zaccai
Publisher : Cambridge University Press
Release : 2017-05-18
ISBN : 1107056373
Language : En, Es, Fr & De

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Book Description :

A comprehensive graduate textbook explaining key physical methods in biology, reflecting the very latest research in this fast-moving field.

Molecular Biophysics for the Life Sciences

Molecular Biophysics for the Life Sciences Book
Author : Norma Allewell,Linda O. Narhi,Ivan Rayment
Publisher : Springer Science & Business Media
Release : 2013-09-28
ISBN : 1461485487
Language : En, Es, Fr & De

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Book Description :

This volume provides an overview of the development and scope of molecular biophysics and in-depth discussions of the major experimental methods that enable biological macromolecules to be studied at atomic resolution. It also reviews the physical chemical concepts that are needed to interpret the experimental results and to understand how the structure, dynamics, and physical properties of biological macromolecules enable them to perform their biological functions. Reviews of research on three disparate biomolecular machines—DNA helicases, ATP synthases, and myosin--illustrate how the combination of theory and experiment leads to new insights and new questions.

Hydrogen Exchange Mass Spectrometry of Proteins

Hydrogen Exchange Mass Spectrometry of Proteins Book
Author : David D. Weis
Publisher : John Wiley & Sons
Release : 2016-01-12
ISBN : 1118703731
Language : En, Es, Fr & De

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Book Description :

Hydrogen exchange mass spectrometry is widely recognized for its ability to probe the structure and dynamics of proteins. The application of this technique is becoming widespread due to its versatility for providing structural information about challenging biological macromolecules such as antibodies, flexible proteins and glycoproteins. Although the technique has been around for 25 years, this is the first definitive book devoted entirely to the topic. Hydrogen Exchange Mass Spectrometry of Proteins: Fundamentals, Methods and Applications brings into one comprehensive volume the theory, instrumentation and applications of Hydrogen Exchange Mass Spectrometry (HX-MS) - a technique relevant to bioanalytical chemistry, protein science and pharmaceuticals. The book provides a solid foundation in the basics of the technique and data interpretation to inform readers of current research in the method, and provides illustrative examples of its use in bio- and pharmaceutical chemistry and biophysics In-depth chapters on the fundamental theory of hydrogen exchange, and tutorial chapters on measurement and data analysis provide the essential background for those ready to adopt HX-MS. Expert users may advance their current understanding through chapters on methods including membrane protein analysis, alternative proteases, millisecond hydrogen exchange, top-down mass spectrometry, histidine exchange and method validation. All readers can explore the diversity of HX-MS applications in areas such as ligand binding, membrane proteins, drug discovery, therapeutic protein formulation, biocomparability, and intrinsically disordered proteins.

Methods in Modern Biophysics

Methods in Modern Biophysics Book
Author : Bengt Nölting
Publisher : Springer Science & Business Media
Release : 2009-10-05
ISBN : 3642030211
Language : En, Es, Fr & De

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Book Description :

Incorporating dramatic recent advances, "Methods in Modern Biophysics" presents a fresh and timely introduction to modern biophysical methods. This innovative text surveys and explains the ten key biophysical methods, including those related to biophysical nanotechnology, scanning probe microscopy, X-ray crystallography, ion mobility spectrometry, mass spectrometry, and proteomics. Containing much information previously unavailable in tutorial form, "Methods in Modern Biophysics" employs worked examples and more than 260 illustrations to fully detail the techniques and their underlying mechanisms. The book was written for advanced undergraduate and graduate students, postdocs, researchers, lecturers and professors in biophysics, biochemistry, general biology and related fields.

Biophysical Methods for Biotherapeutics

Biophysical Methods for Biotherapeutics Book
Author : Tapan K. Das
Publisher : John Wiley & Sons
Release : 2014-02-27
ISBN : 1118354680
Language : En, Es, Fr & De

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Book Description :

With a focus on practical applications of biophysical techniques, this book links fundamental biophysics to the process of biopharmaceutical development. • Helps formulation and analytical scientists in pharma and biotech better understand and use biophysical methods • Chapters organized according to the sequential nature of the drug development process • Helps formulation, analytical, and bioanalytical scientists in pharma and biotech better understand and usestrengths and limitations of biophysical methods • Explains how to use biophysical methods, the information obtained, and what needs to be presented in a regulatory filing, assess impact on quality and immunogenicity • With a focus on practical applications of biophysical techniques, this book links fundamental biophysics to the process of biopharmaceutical development.

Biophysics for Therapeutic Protein Development

Biophysics for Therapeutic Protein Development Book
Author : Linda O. Narhi
Publisher : Springer Science & Business Media
Release : 2013-02-26
ISBN : 1461443164
Language : En, Es, Fr & De

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Book Description :

This book can be used to provide insight into this important application of biophysics for those who are planning a career in protein therapeutic development, and for those outside this area who are interested in understanding it better. The initial chapters describe the underlying theory, and strengths and weaknesses of the different techniques commonly used during therapeutic development. The majority of the chapters discuss the applications of these techniques, including case studies, across the product lifecycle from early discovery, where the focus is on identifying targets, and screening for potential drug product candidates, through expression and purification, large scale production, formulation development, lot-to-lot comparability studies, and commercial support including investigations.

Single Molecule Cellular Biophysics

Single Molecule Cellular Biophysics Book
Author : Mark C. Leake
Publisher : Cambridge University Press
Release : 2013-01-31
ISBN : 1139619349
Language : En, Es, Fr & De

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Book Description :

Recent advances in single molecule science have presented a new branch of science: single molecule cellular biophysics, combining classical cell biology with cutting-edge single molecule biophysics. This textbook explains the essential elements of this new discipline, from the state-of-the-art single molecule techniques to real-world applications in unravelling the inner workings of the cell. Every effort has been made to ensure the text can be easily understood by students from both the physical and life sciences. Mathematical derivations are kept to a minimum whilst unnecessary biological terminology is avoided and text boxes provide readers from either background with additional information. 100 end-of-chapter exercises are divided into those aimed at physical sciences students, those aimed at life science students and those that can be tackled by students from both disciplines. The use of case studies and real research examples make this textbook indispensable for undergraduate students entering this exciting field.

Hydrogen Exchange Mass Spectrometry of Proteins

Hydrogen Exchange Mass Spectrometry of Proteins Book
Author : David D. Weis
Publisher : John Wiley & Sons
Release : 2016-01-11
ISBN : 1118703693
Language : En, Es, Fr & De

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Book Description :

Hydrogen exchange mass spectrometry is widely recognized for its ability to probe the structure and dynamics of proteins. The application of this technique is becoming widespread due to its versatility for providing structural information about challenging biological macromolecules such as antibodies, flexible proteins and glycoproteins. Although the technique has been around for 25 years, this is the first definitive book devoted entirely to the topic. Hydrogen Exchange Mass Spectrometry of Proteins: Fundamentals, Methods and Applications brings into one comprehensive volume the theory, instrumentation and applications of Hydrogen Exchange Mass Spectrometry (HX-MS) - a technique relevant to bioanalytical chemistry, protein science and pharmaceuticals. The book provides a solid foundation in the basics of the technique and data interpretation to inform readers of current research in the method, and provides illustrative examples of its use in bio- and pharmaceutical chemistry and biophysics In-depth chapters on the fundamental theory of hydrogen exchange, and tutorial chapters on measurement and data analysis provide the essential background for those ready to adopt HX-MS. Expert users may advance their current understanding through chapters on methods including membrane protein analysis, alternative proteases, millisecond hydrogen exchange, top-down mass spectrometry, histidine exchange and method validation. All readers can explore the diversity of HX-MS applications in areas such as ligand binding, membrane proteins, drug discovery, therapeutic protein formulation, biocomparability, and intrinsically disordered proteins.

Methods in Modern Biophysics

Methods in Modern Biophysics Book
Author : Bengt Nölting
Publisher : Springer
Release : 2003-09-11
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Incorporating dramatic recent advances, this textbook presents a fresh and timely introduction to modern biophysical methods. An array of new, faster and structurally higher-resolving power biophysical methods now enables scientists to examine the examination of the mysteries of life at a molecular level. So students and researchers alike need to know the technological details behind the latest methods so they can choose appropriate tools and make optimal use of them. This innovative text surveys and explains the ten key biophysical methods, including those related to biophysical nanotechnology, scanning probe microscopy, X-ray crystallography, ion mobility spectrometry, mass spectrometry, and proteomics. Containing much information previously unavailable in tutorial form, Methods in Modern Biophysics employs worked examples and more than 260 illustrations to fully detail the techniques and their underlying mechanisms. The book was written for advanced undergraduate and graduate students, postdocs, researchers, lecturers and professors in biophysics, biochemistry, general biology and related fields.

Progress in Biophysics and Biophysical Chemistry

Progress in Biophysics and Biophysical Chemistry Book
Author : J. A. V. Butler,B. Katz
Publisher : Elsevier
Release : 2016-06-06
ISBN : 1483157032
Language : En, Es, Fr & De

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Book Description :

Progress in Biophysics and Biophysical Chemistry, Volume 7 focuses on the applications of physical or physicochemical ideas and methods to biological problems, including the use of isotopes to investigate metabolic processes. Other subjects discussed in detail are the electric organs of fishes; the thermodynamics of agglutination of red cells; muscle structure and function; and the structure of bone. This book is comprised of seven chapters and begins with a review of the mechanisms of discharge of electric organs in fish in the contexts of general and comparative electrophysiology, paying particular attention to synaptic excitability and the involvement of several electrogenic components in the reflex discharge. The evolution of electric organs in fish is also discussed. The following chapters explore the thermodynamics of isohemagglutinins; use of labeled plasma proteins to study nutritional problems; use of isotopes to analyze intermediary metabolism; and X-crystal analysis of bone. The final two chapters are devoted to muscle structure and theories of contraction, chloroplast structure, and energy conversion in photosynthesis. This volume will be of interest to biophysicists, physicists, and physical chemists working with biological materials.

Applied Biophysics for Drug Discovery

Applied Biophysics for Drug Discovery Book
Author : Donald Huddler,Edward R. Zartler
Publisher : John Wiley & Sons
Release : 2017-07-14
ISBN : 1119099498
Language : En, Es, Fr & De

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Book Description :

Applied Biophysics for Drug Discovery is a guide to new techniques and approaches to identifying and characterizing small molecules in early drug discovery. Biophysical methods are reasserting their utility in drug discovery and through a combination of the rise of fragment-based drug discovery and an increased focus on more nuanced characterisation of small molecule binding, these methods are playing an increasing role in discovery campaigns. This text emphasizes practical considerations for selecting and deploying core biophysical method, including but not limited to ITC, SPR, and both ligand-detected and protein-detected NMR. Topics covered include: • Design considerations in biophysical-based lead screening • Thermodynamic characterization of protein-compound interactions • Characterizing targets and screening reagents with HDX-MS • Microscale thermophoresis methods (MST) • Screening with Weak Affinity Chromatography • Methods to assess compound residence time • 1D-NMR methods for hit identification • Protein-based NMR methods for SAR development • Industry case studies integrating multiple biophysical methods This text is ideal for academic investigators and industry scientists planning hit characterization campaigns or designing and optimizing screening strategies.

Biophysical Chemistry

Biophysical Chemistry Book
Author : Alan Cooper
Publisher : Royal Society of Chemistry
Release : 2004
ISBN : 9780854044801
Language : En, Es, Fr & De

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Book Description :

This book will be ideal for early undergraduates studying chemical or physical sciences and will act as a basis for more advanced study.