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Basic Molecular Protocols In Neuroscience

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Basic Molecular Protocols in Neuroscience

Basic Molecular Protocols in Neuroscience Book
Author : John T. Corthell
Publisher : Academic Press
Release : 2014-04-16
ISBN : 9780128014615
Language : En, Es, Fr & De

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Book Description :

Basic Neuroscience Protocols: Tips, Tricks, and Pitfalls contains explanatory sections that describe the techniques and what each technique really tells the researcher on a scientific level. These explanations describe relevant controls, troubleshooting, and reaction components for some of the most widely used neuroscience protocols that remain difficult for many neuroscientists to implement successfully. Having this additional information will help researchers ensure that their experiments work the first time, and will also minimize the time spent working on a technique only to discover that the problem was them, and not their materials. Describes techniques in very specific detail with Step-by-step instructions, giving researchers in depth understanding Offers many details not present in other protocol books Describes relevant controls for each technique and what those controls mean Chapters include references (key articles, books, protocols) for additional study Describes both the techniques and the habits necessary to get quality results, such as aseptic technique, aliquoting, and general laboratory rules

Basic Molecular Protocols in Neuroscience Tips Tricks and Pitfalls

Basic Molecular Protocols in Neuroscience  Tips  Tricks  and Pitfalls Book
Author : John T. Corthell
Publisher : Academic Press
Release : 2014-04-16
ISBN : 0128015276
Language : En, Es, Fr & De

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Book Description :

Basic Neuroscience Protocols: Tips, Tricks, and Pitfalls contains explanatory sections that describe the techniques and what each technique really tells the researcher on a scientific level. These explanations describe relevant controls, troubleshooting, and reaction components for some of the most widely used neuroscience protocols that remain difficult for many neuroscientists to implement successfully. Having this additional information will help researchers ensure that their experiments work the first time, and will also minimize the time spent working on a technique only to discover that the problem was them, and not their materials. Describes techniques in very specific detail with step-by-step instructions, giving researchers in-depth understanding Offers many details not present in other protocol books Describes relevant controls for each technique and what those controls mean Chapters include references (key articles, books, protocols) for additional study Describes both the techniques and the habits necessary to get quality results, such as aseptic technique, aliquoting, and general laboratory rules

Family Iridoviridae Molecular and Ecological Studies of a Family Infecting Invertebrates and Ectothermic Vertebrates

Family Iridoviridae Molecular and Ecological Studies of a Family Infecting Invertebrates and Ectothermic Vertebrates Book
Author : V. Gregory Chinchar,Amanda LJ Duffus
Publisher : MDPI
Release : 2019-10-01
ISBN : 3039215167
Language : En, Es, Fr & De

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Book Description :

Ranaviruses and other viruses within the family Iridoviridae, infect a wide range of ecologically and commercially important ectothermic vertebrates, i.e., bony fish, amphibians, and reptiles, and invertebrates, including agricultural and medical pests and cultured shrimp and crayfish, and are responsible for considerable morbidity and mortality. Understanding the impact of these various agents on diverse host species requires the combined efforts of ecologists, veterinarians, pathologists, comparative immunologists and molecular virologists. Unfortunately, investigators involved in these studies often work in discipline-specific silos that preclude interaction with others whose insights and approaches are required to comprehensively address problems related to ranavirus/iridovirus disease. Our intent here is to breakdown these silos and provide a forum where diverse researchers with a common interest in ranavirus/iridovirus biology can profitably interact. As a colleague once quipped, “Three people make a genius.” We are hoping to do something along those lines by presenting a collection of research articles dealing with issues of anti-viral immunity, identification of a potentially novel viral genus exemplified by erythrocytic necrosis virus, viral inhibition of innate immunity, identification of novel hosts for lymphocystivirus and invertebrate iridoviruses, and modelling studies of ranavirus transmission. Collectively these and others will exemplify the breadth of ongoing studies focused on this virus family.

Cellular and Molecular Methods in Neuroscience Research

Cellular and Molecular Methods in Neuroscience Research Book
Author : Adalberto Merighi,Giorgio Carmignoto
Publisher : Springer Science & Business Media
Release : 2007-12-14
ISBN : 0387224602
Language : En, Es, Fr & De

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Book Description :

There are numerous books on cellular and molecular protocols for general use in cell biology but very few are exclusively devoted to neurobiology. This book fills this gap and explains in a clear and consistent manner, some of the more commonly used protocols in neuroscience research. Each chapter is written by either the person who invented the procedure or an expert in the field. The format is uniform: "Overview," "Background," "Protocols," and "results and discussion." Each protocol begins with the principle of the technique, studies in cell culture, materials and reagents, and, lastly, step-by-step outline of the procedure itself. This highly practical book is also well illustrated (with 17 four color plates) to make the concepts and procedures easy to understand and perform.

Immunotherapy for Gastrointestinal Malignancies

Immunotherapy for Gastrointestinal Malignancies Book
Author : Ramakrishna Vadde
Publisher : Springer Nature
Release : 2021-10-19
ISBN : 9811564876
Language : En, Es, Fr & De

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Book Description :

Download Immunotherapy for Gastrointestinal Malignancies book written by Ramakrishna Vadde, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

NMDA Receptor Protocols

NMDA Receptor Protocols Book
Author : Min Li
Publisher : Springer Science & Business Media
Release : 1999
ISBN : 1592596835
Language : En, Es, Fr & De

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Book Description :

In NMDA Receptor Protocols, Min Li and a panel of hands-on experimentalists detail state-of-the-art molecular techniques for studying NMDA ligand-gated ion channels and developing assays for nontherapeutic lead selection. The topics range from cDNA cloning to in vitro and in vivo investigation of the channel complex in the mammalian brain. Additional topics include the biochemical analysis of the channel protein and the construction of various heterologous systems for both basic research and high throughput screens (HTS) for pharmaceutical chemicals. Although the focus is on NMDA receptors, the methods are applicable to other ligand-gated ion channels and with some modification may be extended to related membrane receptors or signaling receptors. NMDA Receptor Protocols provides today's scientists at all skill levels a diverse collection of highly reproducible molecular techniques for ion channel research and biotherapeutic development. Eminently practical and reproducible, these techniques offer powerful methods for basic research on NMDA receptor structure and function, as well as enormous opportunities for clinical investigation toward the development of novel bioactive compounds.

Short Protocols in Neuroscience

Short Protocols in Neuroscience Book
Author : Charles R. Gerfen,Michael A. Rogawski,David R. Sibley,Phil Skolnick,Susan Wray
Publisher : Current Protocols
Release : 2006-08-04
ISBN : 9780471783992
Language : En, Es, Fr & De

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Book Description :

Short Protocols in Neuroscience: Cellular and Molecular Methods is an easy-to-use compendium of cellular and molecular methods from Current Protocols in Neuroscience, part of the acclaimed series that has set the standard for excellence for laboratory manuals. The title provides a portable and streamlined at-the-bench resource, covering methods for analysis of neural cell structure and function, isolation and culture of neural cells, and characterization of neural cell gene expression.

Current Protocols in Neuroscience

Current Protocols in Neuroscience Book
Author : Jacqueline N. Crawley,Charles Gerfen,Ron McKay,Michael Rogawski,David R. Sibley
Publisher : Current Protocols
Release : 1997-06-01
ISBN : 9780471163619
Language : En, Es, Fr & De

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Book Description :

Current Protocols in Neuroscience (CPN) draws from techniques in molecular neurobiology, neurophysiology, neuroanatomy, neuropharmacology, and behavioral neuroscience to meet the specific needs of researchers in the full range of disciplines that is involved in studying the brain, nervous system, and corresponding behaviors. The editorial board of CPN have assembled an outstanding range of methods to enable users to explore their fields in greater depth and branch into related areas. The one-volume, looseleaf manual features carefully edited techniques with authors' troubleshooting tips and helpful comments that come from extensive experience in using these procedures. Quarterly updates, filed into the looseleaf, keep you and your laboratory current with the latest developments in this rapidly changing field. The initial purchase includes one year of updates and then subscribers may renew their annual subscriptions. Current Protocols publishes a family of laboratory manuals for bioscientists, including Molecular Biology, Immunology, Human Genetics, Protein Science, Cytometry, Cell Biology, Pharmacology, and Toxicology.

Protocols in Molecular Neurobiology

Protocols in Molecular Neurobiology Book
Author : Alan Longstaff,Patricia Revest
Publisher : Humana Press
Release : 2013-08-15
ISBN : 9781489940193
Language : En, Es, Fr & De

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Book Description :

Neurobiologists are bound to differ in their perceptions of what the discipline of molecular neurobiology should encompass. We have taken the view that molecular neurobiology should cover any aspect of brain science that uses the techniques of modern molecular biology, though we accept the fact that classification of a technique as a "biochemical* or "molecular biological* one is in itself somewhat arbitrary. Each chapter of this volume sets out to identify a clear problem in neurobiology and to place it in its context within the literatu- i.e., indicating how the solution of the problem will advance knowledge in the field. The core of the chapter then details the approaches taken to solve the problem, in sufficient detail that the reader can appre- ate exactly why a specific strategy was adopted and how it was executed. Each chapter also includes detailed protocols providing all the inf- mation necessary to reproduce the technique and its results in any appropriately equipped laboratory.

Protocols used in Molecular Biology

Protocols used in Molecular Biology Book
Author : Sandeep Singh,Dhiraj Kumar
Publisher : Bentham Science Publishers
Release : 2020-01-23
ISBN : 981143929X
Language : En, Es, Fr & De

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Book Description :

Protocols used in Molecular Biology is a compilation of several examples of molecular biology protocols. Each example is presented with a concise introduction, materials and chemicals required, a step-by-step procedure and troubleshooting tips. Information about the application of the protocol is also provided. The techniques included in this book are essential to research in the fields of proteomics, genomics, cell culture, epigenetic modification and structural biology. The protocols can also be used by clinical researchers (neuroscientists and oncologists, for example) for medical applications (diagnostics, therapeutics and multidisciplinary projects).

Guide to Research Techniques in Neuroscience

Guide to Research Techniques in Neuroscience Book
Author : Matt Carter,Jennifer C. Shieh
Publisher : Academic Press
Release : 2015-02-27
ISBN : 0128005971
Language : En, Es, Fr & De

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Book Description :

Neuroscience is, by definition, a multidisciplinary field: some scientists study genes and proteins at the molecular level while others study neural circuitry using electrophysiology and high-resolution optics. A single topic can be studied using techniques from genetics, imaging, biochemistry, or electrophysiology. Therefore, it can be daunting for young scientists or anyone new to neuroscience to learn how to read the primary literature and develop their own experiments. This volume addresses that gap, gathering multidisciplinary knowledge and providing tools for understanding the neuroscience techniques that are essential to the field, and allowing the reader to design experiments in a variety of neuroscience disciplines. Written to provide a "hands-on" approach for graduate students, postdocs, or anyone new to the neurosciences Techniques within one field are compared, allowing readers to select the best techniques for their own work Includes key articles, books, and protocols for additional detailed study Data analysis boxes in each chapter help with data interpretation and offer guidelines on how best to represent results Walk-through boxes guide readers step-by-step through experiments

Methods in Genomic Neuroscience

Methods in Genomic Neuroscience Book
Author : Hemin R. Chin,Steven O. Moldin
Publisher : CRC Press
Release : 2001-09-26
ISBN : 100061171X
Language : En, Es, Fr & De

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Book Description :

The past few years have witnessed extraordinary advances in molecular genetic techniques and the accumulation of structural genomics information and resources in both human and model organisms. With the development of new technologies and the availability of resources like the sequence of eukaryotic genomes, problems of a previously unthinkable sco

Molecular Neuroscience

Molecular Neuroscience Book
Author : Rusty Lansford
Publisher : Unknown
Release : 2014-09-30
ISBN : 9781621820147
Language : En, Es, Fr & De

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Book Description :

A wide variety of powerful molecular techniques have been applied to biology in recent decades, ranging from recombinant DNA technologies to state-of-the-art imaging methods. But the plethora of techniques available combined with the complexities of neurobiological systems can make it difficult for neuroscientists to select and carry out an experimental procedure to effectively address the question at hand. This laboratory manual serves as a comprehensive practical guide to molecular and cellular methods for neuroscientists. It consists of five major sections: Working with Cells, Working with DNA, Working with RNA, Gene Transfer, and Imaging. Each includes step-by-step protocols and discussions of basic and cutting-edge procedures for working in that area. Fundamental techniques include maintaining a sterile working environment, purifying and culturing neural cells, isolating and manipulating DNA and RNA, and understanding and using a microscope. Advanced topics include single-neuron isolation and analysis, in vivo gene delivery and imaging, optogenetics, RNA interference, transgenic technologies, high-throughput analysis of gene expression (e.g., RNA-Seq), and constructing and imaging fluorescent proteins. The manual includes protocols developed in the Advanced Techniques in Molecular Neuroscience course offered annually at Cold Spring Harbor Laboratory, as well as protocols drawn from its best-selling lab manuals. It is an essential resource for all neuroscientists, from graduate students upward, who seek to use molecular techniques to probe the complexities of the nervous system.

Molecular Neuroscience

Molecular Neuroscience Book
Author : Patricia Revest,Alan Longstaff
Publisher : Garland Science
Release : 1998
ISBN : 1859962505
Language : En, Es, Fr & De

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Book Description :

Molecular Neuroscience takes an interesting and original approach to explaining the present and future impact of molecular biology on the study of neuroscience. It provides the reader with a level of understanding which will allow them to delve deeper into the ever expanding neuroscience literature. A good balance is achieved between a didactic style and well-selected examples of experimental approaches. This book is written for 2nd and 3rd year life science undergraduates and MSc students on a range of courses. Medical students and researchers requiring a concise introduction to neuroscience, particularly the latest molecular approaches, will also find it an essential read.

Programmed Cells from Basic Neuroscience to Therapy

Programmed Cells from Basic Neuroscience to Therapy Book
Author : Fred H. Gage,Yves Christen
Publisher : Springer
Release : 2016-10-01
ISBN : 9783662512944
Language : En, Es, Fr & De

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Book Description :

The recent advances in Programming Somatic Cell (PSC) including induced Pluripotent Stem Cells (iPS) and Induced Neuronal phenotypes (iN), has changed our experimental landscape and opened new possibilities. The advances in PSC have provided an important tool for the study of human neuronal function as well as neurodegenerative and neurodevelopmental diseases in live human neurons in a controlled environment. For example, reprogramming cells from patients with neurological diseases allows the study of molecular pathways particular to specific subtypes of neurons such as dopaminergic neurons in Parkinson’s Disease, Motor neurons for Amyolateral Sclerosis or myelin for Multiple Sclerosis. Detecting disease-specific molecular signatures in live human brain cells, opens possibilities for early intervention therapies and new diagnostic tools. Importantly, once the neurological neural phenotype is detected in vitro, the so-called “disease-in-a-dish” approach allows for the screening of drugs that can ameliorate the disease-specific phenotype. New therapeutic drugs could either act on generalized pathways in all patients or be patient-specific and used in a personalized medicine approach. However, there are a number of pressing issues that need to be addressed and resolved before PSC technology can be extensively used for clinically relevant modeling of neurological diseases. Among these issues are the variability in PSC generation methods, variability between individuals, epigenetic/genetic instability and the ability to obtain disease-relevant subtypes of neurons . Current protocols for differentiating PSC into specific subtypes of neurons are under development, but more and better protocols are needed. Understanding the molecular pathways involved in human neural differentiation will facilitate the development of methods and tools to enrich and monitor the generation of specific subtypes of neurons that would be more relevant in modeling different neurological diseases.

Marine Disease Ecology

Marine Disease Ecology Book
Author : Kevin D. Lafferty
Publisher : Oxford University Press, USA
Release : 2020-01-30
ISBN : 0198821638
Language : En, Es, Fr & De

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Book Description :

Whether through loss of habitat or cascading community effects, diseases can shape the very nature of the marine environment. Despite their significant impacts, studies of marine diseases have tended to lag behind their terrestrial equivalents, particularly with regards to their ecological effects. However, in recent decades global research focused on marine disease ecology has expanded at an accelerating rate. This is due in part to increases in disease emergence across many taxa, but can also be attributed to a broader realization that the parasites responsible for disease are themselves important members of marine communities. Understanding their ecological relationships with the environment and their hosts is critical to understanding, conserving, and managing natural and exploited populations, communities, and ecosystems. Courses on marine disease ecology are now starting to emerge and this first textbook in the field will be ideally placed to serve them. Marine Disease Ecology is suitable for graduate students and researchers in the fields of marine disease ecology, aquaculture, fisheries, veterinary science, evolution and conservation. It will also be of relevance and use to a broader interdisciplinary audience of government agencies, NGOs, and marine resource managers.

Protocols for Gene Transfer in Neuroscience

Protocols for Gene Transfer in Neuroscience Book
Author : P. R. Lowenstein,L. W. Enquist
Publisher : John Wiley & Sons Incorporated
Release : 1996-07-25
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Protocols for Gene Transfer in Neuroscience Towards Gene Therapy of Neurological Disorders Edited by P. R. Lowenstein University of Manchester, UK and L. W. Enquist Princeton University, USA The brain and the cells that compose it have always presented unique barriers to any kind of outside interventions, including drug delivery, surgical procedures and the genetic manipulation of cells. However, these barriers are now being bypassed by the new technology of gene delivery. Protocols for Gene Transfer in Neuroscience is the first practical guide to gene delivery, providing over 150 easy to follow, step-by-step protocols, together with troubleshooting hints and the theoretical background needed for neuroscientists to understand, adapt and expand the technology as needed. Written by a team of international expert neuroscientists, geneticists, molecular biologists and biochemists, Protocols for Gene Transfer in Neuroscience describes the latest techniques from the top gene-delivery labs, ranging from physical transfection techniques, through the use of a wide range of viral vectors, to retrovirally transduced cell and bone marrow transplantation. Several variations of a single protocol are given, thus demonstrating how different labs have solved a common problem in different ways. The protocols are also indexed and cross-referenced so that an investigator can find all the details required for a particular problem.

Neuroscience Protocols

Neuroscience Protocols Book
Author : Anonim
Publisher : Unknown
Release : 1993
ISBN : 9780444818027
Language : En, Es, Fr & De

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Book Description :

Download Neuroscience Protocols book written by , available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Neural Development

Neural Development Book
Author : Renping Zhou,Lin Mei
Publisher : Humana Press
Release : 2016-08-23
ISBN : 9781493960187
Language : En, Es, Fr & De

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Book Description :

Understanding the molecular and cellular mechanisms underlying the development of specific neural circuits is not just an intellectual curiosity but also central to our ability to develop therapeutic approaches to repair damaged pathways in the future. In Neural Development: Methods and Protocols, experts in the field contribute commonly used protocols to facilitate future research in developmental neuroscience. Split into four convenient sections, this detailed volume covers techniques of culturing neurons and glia as well as their growth and differentiation, methods of gene delivery and down regulation, protocols for analyzing axon growth and guidance plus synapse formation, and, finally, basic methods to analyze brain morphology and axon pathways in developing animals. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and accessible, Neural Development: Methods and Protocols provides key guidance for students and postdoctoral fellows new to developmental neurobiology.

Receptor Molecular Biology

Receptor Molecular Biology Book
Author : Anonim
Publisher : Elsevier
Release : 1995-03-22
ISBN : 9780080536446
Language : En, Es, Fr & De

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Book Description :

The volumes in this series include contemporary techniques significant to a particular branch of neuroscience. They are an invaluable aid to the student as well as the experienced researcher not only in developing protocols in neuroscience but in disciplines where research is becoming closely related to neuroscience. Each volume of Methods in Neurosciences contains an index, and each chapter includes references. Dr. Conn became Editor-in-Chief of the series beginning with Volume 15, so each subsequent volume could be guest-edited by an expert in that specific field. This further strengthens the depth of coverage in Methods in Neurosciences for students and researchers alike. Cloning Expression systems Signal transduction Structure-function techniques Antireceptor antibodies Regulation 3-D receptor modeling and computational probing