Skip to main content

Bacterial Cellular Metabolic Systems

In Order to Read Online or Download Bacterial Cellular Metabolic Systems Full eBooks in PDF, EPUB, Tuebl and Mobi you need to create a Free account. Get any books you like and read everywhere you want. Fast Download Speed ~ Commercial & Ad Free. We cannot guarantee that every book is in the library!

Bacterial Cellular Metabolic Systems

Bacterial Cellular Metabolic Systems Book
Author : K. Shimizu
Publisher : Elsevier
Release : 2013-03-26
ISBN : 1908818204
Language : En, Es, Fr & De

GET BOOK

Book Description :

The metabolic regulation of a cell system is of critical importance in systems biology, and a robust model of these mechanisms is essential in predicting the effects on the metabolism of both the culture environment and the knockout of specific genes. Bacterial cellular metabolic systems focuses on this highly topical subject in relation to culture environment and provides a detailed analysis from gene level to metabolic level regulation, as well as offering a discussion of the most recent modelling approaches. The book begins with an introduction to metabolic mechanisms and to the metabolic regulation of a cell, before moving on to discussing the action of global regulators in response to a specific culture environment. The second half of the book examines conventional flux balance analysis and its applications, 13C-metabolic flux analysis, and the effect of a specific gene knockout on the metabolism. Comprehensive account of metabolic regulation via global regulators in response to changes in the culture environment Basic formulation of 13C-metabolic flux analysis based on 13C-labelling experiments Systems biology approach for the modelling and computer simulation of the main metabolic pathways of a cell system

Bacterial Metabolism

Bacterial Metabolism Book
Author : Gerhard Gottschalk
Publisher : Springer Science & Business Media
Release : 2012-12-06
ISBN : 1461210720
Language : En, Es, Fr & De

GET BOOK

Book Description :

This concise yet comprehensive text surveys the field of bacterial metabolism in terms useful to students and researchers. Emphasis is on those metabolic reactions occurring only in bacteria. Thus, the book describes in detail the energy metabolism of the various groups of bacteria. In addition, it examines pathways used by bacteria for the degradation of organic compounds, the synthesis of cellular constituents, the regulation of bacterial metabolism and the fixation of molecular nitrogen.

Engineering Microbial Metabolism For Chemical Synthesis Reviews And Perspectives

Engineering Microbial Metabolism For Chemical Synthesis  Reviews And Perspectives Book
Author : Yan Yajun
Publisher : World Scientific
Release : 2017-12-26
ISBN : 1786344319
Language : En, Es, Fr & De

GET BOOK

Book Description :

Microbial metabolism refers to how microbes obtain energy and nutrients needed to live and reproduce. Its uses in chemical synthesis are multiple, and, when engineered, can provide reliable and reproducible reactions for application in industrial or biogeochemical processes. This book provides expert reviews and perspectives on how to engineer microbial metabolism for chemical synthesis. Major metabolic pathways or networks in microbial systems, including glycolysis, citric acid and photosynthesis, are briefly summarized. Following this, the metabolic engineering efforts of extending these pathways and networks for the biosynthesis of various chemicals are reviewed with the emphasis on the biochemical reactions and engineering strategies. The potential of these pathways for further metabolic engineering are also discussed. From graduate to professional level, cellular metabolism and metabolic engineering applications are introduced to the readers gradually and systematically, making it perfect for students, researchers and practitioners of chemistry, biochemistry and metabolic engineering. Contents: Glycolysis and Its Metabolic Engineering Applications (Jian Wang and Yajun Yan)Citric Acid Cycle and Its Metabolic Engineering Applications (Jia Wang and Xiaolin Shen)Amino Acid Biosynthesis and Its Metabolic Engineering Applications (Yi-Xin Huo)Fatty Acid Biosynthesis and Its Metabolic Engineering Applications (Yi Liu and Tiangang Liu)Photosynthesis and Its Metabolic Engineering Applications (Jason T Ku and Ethan I Lan)Pentose Phosphate Pathway and Its Metabolic Engineering Applications (Ying Wang and Chun Li)Mevalonate/2-Methylerythritol 4-Phosphate Pathways and Their Metabolic Engineering Applications (Xinxiao Sun and Qipeng Yuan)Xylose Metabolism and Its Metabolic Engineering Applications (Maria K McClintock and Kechun Zhang)Engineering Metabolism for the Synthesis of Polyhydroxyalkanoate Biopolymers (Guo-Qiang Chen and Xiao-Ran Jiang) Readership: Graduate students, researchers and practitioners of chemistry, biochemistry and metabolic engineering. Keywords: Microbial Metabolism;Chemical Synthesis;Biosynthesis;Biochemical Reactions;Photosynthesis;Metabolic Engineering;Cellular Metabolism;GlycolysisReview:0

Systems and Synthetic Metabolic Engineering

Systems and Synthetic Metabolic Engineering Book
Author : Yanfeng Liu,Guocheng Du,Long Liu
Publisher : Academic Press
Release : 2020-07-10
ISBN : 0128217529
Language : En, Es, Fr & De

GET BOOK

Book Description :

Systems and Synthetic Metabolic Engineering provides an overview of the development of metabolic engineering within medicine that is fueled by systems and synthetic biology. These newly developed, successful strategies of metabolic engineering guide the audience on how to propose and test proper strategies for metabolic engineering research. In addition to introductory, regulatory and challenges in the field, the book also covers dynamic control and autonomous regulation to control cell metabolism, along with computational modeling and industrial applications. The book is written by leaders in the field, making it ideal for synthetic biologists, researchers, students and anyone working in this area. Discusses the current progress of metabolic engineering, focusing on systems biology and synthetic biology Covers introductory, regulatory, strategies, production and challenges in the field Written technically for synthetic biologists, researchers, students, industrialists, policymakers and stakeholders

Systems Metabolic Engineering

Systems Metabolic Engineering Book
Author : Christoph Wittmann,Sang Yup Lee
Publisher : Springer Science & Business Media
Release : 2012-06-15
ISBN : 9400745346
Language : En, Es, Fr & De

GET BOOK

Book Description :

Systems Metabolic Engineering is changing the way microbial cell factories are designed and optimized for industrial production. Integrating systems biology and biotechnology with new concepts from synthetic biology enables the global analysis and engineering of microorganisms and bioprocesses at super efficiency and versatility otherwise not accessible. Without doubt, systems metabolic engineering is a major driver towards bio-based production of chemicals, materials and fuels from renewables and thus one of the core technologies of global green growth. In this book, Christoph Wittmann and Sang-Yup Lee have assembled the world leaders on systems metabolic engineering and cover the full story – from genomes and networks via discovery and design to industrial implementation practises. This book is a comprehensive resource for students and researchers from academia and industry interested in systems metabolic engineering. It provides us with the fundaments to targeted engineering of microbial cells for sustainable bio-production and stimulates those who are interested to enter this exiting research field.

Developing an Individual based Model to Study the Bacterial Denitrification Process

Developing an Individual based Model to Study the Bacterial Denitrification Process Book
Author : Pablo Alejandro Araujo Granda
Publisher : Unknown
Release : 2017
ISBN : 0987650XXX
Language : En, Es, Fr & De

GET BOOK

Book Description :

It is crucial to study the denitrification process driven by bacteria as it is one of the most important environmental processes for several reasons: i) it has an application in the removal of nitrogen (N) from high-N waste materials ii), it is one of the mechanisms to N-fertilizer¿s loss, iii) it contributes to the emissions of gasses with large global warming potential, and iv) it is the mechanism by which the global nitrogen cycle is balanced. Many models have been developed in the framework of continuous models to deal with the complexity of the denitrification process in order to become predictive models, but some of the assumptions contained in them are not realistic enough in those contexts, and also they have their own constraints and limitations. On the other hand, the researchers have paid more attention to the role of microbial activity with the advance of experimental techniques. Discrete models, such as individual-based models (IBMs), can be developed and applied to microbial systems due to the fact that they allow representation of some intracellular characteristics regarding the complexity of the micro-organisms, which constitutes a key advantage of this modelling approach in the study of the different biotechnological processes. The IBM is able to incorporate and accommodate the behaviour of denitrifying bacteria, and investigate their metabolism from different and attractive perspectives. A key factor in modelling the microbial activity is the methodology followed to represent metabolic pathways. A cellular metabolic model could be based on a non-equilibrium thermodynamic approach such as the Thermodynamic Electron Equivalents Model (TEEM), which is developed for biomass yield prediction using the associated standard Gibbs free energies and the bioenergetics growth efficiency between cell anabolism and catabolism. The main objective of this doctoral thesis is to develop an IBM to study denitrification processes driven by denitrifying bacteria, using TEEM to write microbial metabolic reactions (10Rs) which represent the metabolic pathways as the center of the individual sub-model. Two new computational models of the INDISIM family, INDISIM-Paracoccus and INDISIM-Denitrification have been designed, implemented on the NetLogo platform, parameterized and calibrated with experimental data to analyze the system dynamics in a bioreactor in batch and continuous culture with denitrifying bacteria growing in it. The bioreactor conditions can be aerobic and/or anaerobic, and the growing media is liquid medium with an electron donor, C-source, N-source, and oxygen and all N-oxides as electron acceptors. An open access and open source tool has been developed to write 10Rs based on TEEM. It is called MbT-tool which stands for Metabolism-based on Thermodynamics. Using MbT-Tool three sets of 10Rs have been written, which are the centre of the individual sub-model for INDISIM-Paracoccus and INDISIM-denitrification, representing reactions involved in: i) cellular maintenance, ii) individual mass synthesis, and iii) individual mass degradation to reduce cytotoxic products. The simulation results obtained with INDISIM-Paracoccus and INDISIM-Denitrification have been compared with experimental data published by Felgate et al. (2012) regarding the growth of Paracoccus denitrificans and Achromobacter xylosoxidans in a bioreactor. According to the statistical analysis of the simulations results, for both denitrifying bacteria tested, the IBMs developed show better adjustments in the assays with electron donor limited than in the assays with electron acceptor limited.

Metabolism and Bacterial Pathogenesis

Metabolism and Bacterial Pathogenesis Book
Author : Tyrrell Conway,Paul S. Cohen
Publisher : John Wiley & Sons
Release : 2020-07-24
ISBN : 1555818889
Language : En, Es, Fr & De

GET BOOK

Book Description :

Groundbreaking thinking on how bacterial metabolism is foundational to pathogenesis For too long, bacterial metabolism and bacterial pathogenesis have been studied as separate entities. However, the scientific community is beginning to realize that not only are bacterial nutrient acquisition and utilization essential for pathogenesis, but that interfering with the pathogen-specific metabolic pathways used during infection can regulate virulence factor expression and might lead to effective breakthroughs in a variety of treatments. Editors Paul Cohen and Tyrrell Conway, who pioneered the use of metabolic mutants in competitive colonization assays, an approach now widely used to investigate the nutrition of pathogens in vivo, are uniquely qualified to advance our knowledge of this integrative field of research. They convened a group of contributors who are breaking new ground in understanding how bacterial metabolism is foundational to pathogenesis to share their expert perspectives and outlook for the future. Beginning with overviews, Metabolism and Bacterial Pathogenesis covers a wide range of diseases and both Gram-positive and -negative bacteria that serve as model systems for in vitro and in vivo investigations intracellular, respiratory, and enteric pathogens pathogen-specific nutrient acquisition in hosts mechanisms of host-driven metabolic adaptation by pathogens metabolic regulation of virulence gene expression Useful for specialists in bacterial pathogenesis and specialists in metabolism as well as molecular biologists, physicians, veterinarians, dentists, graduate and undergraduate students, and laboratory technicians, Metabolism and Bacterial Pathogenesis is also essential reading for scientists studying the microbiome.

The Living Cell as an Open Thermodynamic System Bacteria and Irreversible Thermodynamics

The Living Cell as an Open Thermodynamic System  Bacteria and Irreversible Thermodynamics Book
Author : Anonim
Publisher : Unknown
Release : 1971
ISBN : 0987650XXX
Language : En, Es, Fr & De

GET BOOK

Book Description :

Little is known about the fundamental cause of cell division of bacteria, even though much information concerning cellular metabolism is available. An hypothesis is presented, considering the living cell as an open thermodynamic system, that provides a general description in terms of nonequilibrium thermodynamics of initiation, continuation of growth, and division of these organisms. The same hypothesis can be applied to yeasts and possibly to mammalian cells. The proposed hypothesis accounts simply for the commonly observed characteristics of the bacterial life cycle. It provides reasons for certain observed anomalies, showing them to be logical events in the proposed theoretical context. Other commonly observed events are shown to be artifacts of the usual investigational techniques.

Metabolic Engineering

Metabolic Engineering Book
Author : Sang Yup Lee,E. Terry Papoutsakis
Publisher : CRC Press
Release : 1999-09-29
ISBN : 9780824773908
Language : En, Es, Fr & De

GET BOOK

Book Description :

This unique reference/text presents the basic theory and practical applications of metabolic engineering (ME). It offers systematic analysis of complex metabolic pathways and ways of employing recombinant DNA techniques to alter cell behavior, metabolic patterns, and product formation. Treating ME as a distinct subfield of genetic engineering, the book demonstrates new means of enabling cells to produce valuable proteins, polypeptides, and primary and secondary metabolites. Written by more than 35 leading international experts in the field, this book discusses metabolic engineering in plant and mammalian cells, bacteria, and yeasts and assesses metabolic engineering applications in agriculture, pharmaceuticals, and environmental systems. It illuminates the potential of the "cell factory" model for production of chemicals and therapeutics and examines methods for developing new antiviral and antibacterial molecules and effective gene and somatic-cell therapies. Metabolic Engineering also addresses the use of metabolic flux analysis, metabolic control analysis, and online metabolic flux analysis.

Biodegradative Bacteria

Biodegradative Bacteria Book
Author : Hideaki Nojiri,Masataka Tsuda,Masao Fukuda,Yoichi Kamagata
Publisher : Springer Science & Business Media
Release : 2013-11-01
ISBN : 4431545204
Language : En, Es, Fr & De

GET BOOK

Book Description :

Biodegradative Bacteria highlights the novel nature of bacterial cell functions in the field of biodegradation by putting them into three parts: (1) Genetic and genomic systems, (2) Degradative enzyme systems, and (3) Bacterial behavior in natural environmental systems. The first part of the book includes cell functions as degradative machinery, genome systems for effective degradation, and the evolution of degradative systems by mobile genetic elements. The second part deals with the structure, function, evolution, diversity, and application of degradative and related enzymes. The third part presents cell or genomic behaviors of biodegradative bacteria in natural ecosystems. Bacterial metabolic capacity, which plays an important role in the global material cycle, contributes significantly to the buffering capacity for the huge and unintended release of various chemicals. Recently, however, the prosperity and globalization of material civilization has led not only to severe local contamination by hazardous chemicals, but also to continuous increment of contaminant concentrations worldwide. To solve such urgent global issues, bacterial functions that are involved in biodegradation of hazardous chemicals have been analyzed. The term “biodegradative bacteria” refers to those bacteria that have the ability to degrade such xenobiotic (man-made) and/or hazardous chemicals. Analyses of biodegradative bacteria include diverse areas of study, such as genetics, enzymology, genomics, cell physiology, ecology, and evolutionary biology. In other words, the targets investigated in research on biodegradative bacteria include single molecules, single cell systems, bacterial consortia (interaction with surrounding microorganisms), and interaction with surrounding biotic and abiotic materials. Such complexity makes the research on biodegradative bacteria difficult but quite interesting.

Bacterial Metabolism

Bacterial Metabolism Book
Author : H. W. Doelle
Publisher : Academic Press
Release : 2014-06-28
ISBN : 1483272370
Language : En, Es, Fr & De

GET BOOK

Book Description :

Bacterial Metabolism, Second Edition describes microbial systematics and microbial chemistry and focuses on catabolic events. This book deals with the progress made in bacterial metabolism that includes data on regulatory mechanisms; comparison of bacterial growth kinetics with enzyme kinetics; aerobic amino acid catabolism; and the glucose transport mechanism. This text also emphasizes the development of photosynthetic phosphorylation in the different bacterial families. This book explains anaerobic respiration and carbohydrate metabolism—glucose, fructose, lactose, mannose, allose, and sorbitol. This text then describes aerobic respiration including the "Nitroso" and "Nitro" groups of genera, and the Knallgas bacteria, which use the reaction between molecular hydrogen and molecular oxygen as their source of energy. This book also explains the microbial transformation of iron as caused by either specific organisms (e.g. Ferrobacillus ferrooxidans) or nonspecific organisms. This selection also explains the process of fermentation by Enterobacteriaceae, lactic acid bacteria, and proteolytic clostridia. This text can be valuable for microchemists, microbiologists, students, and academicians whose disciplines are in biological chemistry and cellular biology.

Microbiology For Dummies

Microbiology For Dummies Book
Author : Jennifer Stearns,Michael Surette
Publisher : John Wiley & Sons
Release : 2019-02-28
ISBN : 1119544416
Language : En, Es, Fr & De

GET BOOK

Book Description :

Microbiology For Dummies (9781119544425) was previously published as Microbiology For Dummies (9781118871188). While this version features a new Dummies cover and design, the content is the same as the prior release and should not be considered a new or updated product. Microbiology is the study of life itself, down to the smallest particle Microbiology is a fascinating field that explores life down to the tiniest level. Did you know that your body contains more bacteria cells than human cells? It's true. Microbes are essential to our everyday lives, from the food we eat to the very internal systems that keep us alive. These microbes include bacteria, algae, fungi, viruses, and nematodes. Without microbes, life on Earth would not survive. It's amazing to think that all life is so dependent on these microscopic creatures, but their impact on our future is even more astonishing. Microbes are the tools that allow us to engineer hardier crops, create better medicines, and fuel our technology in sustainable ways. Microbes may just help us save the world. Microbiology For Dummies is your guide to understanding the fundamentals of this enormously-encompassing field. Whether your career plans include microbiology or another science or health specialty, you need to understand life at the cellular level before you can understand anything on the macro scale. Explore the difference between prokaryotic and eukaryotic cells Understand the basics of cell function and metabolism Discover the differences between pathogenic and symbiotic relationships Study the mechanisms that keep different organisms active and alive You need to know how cells work, how they get nutrients, and how they die. You need to know the effects different microbes have on different systems, and how certain microbes are integral to ecosystem health. Microbes are literally the foundation of all life, and they are everywhere. Microbiology For Dummies will help you understand them, appreciate them, and use them.

Current Challenges in Modeling Cellular Metabolism

Current Challenges in Modeling Cellular Metabolism Book
Author : Daniel Machado,Kai H. Zhuang,Nikolaus Sonnenschein,Markus J. Herrgård
Publisher : Frontiers Media SA
Release : 2016-01-21
ISBN : 2889197549
Language : En, Es, Fr & De

GET BOOK

Book Description :

Mathematical and computational models play an essential role in understanding the cellular metabolism. They are used as platforms to integrate current knowledge on a biological system and to systematically test and predict the effect of manipulations to such systems. The recent advances in genome sequencing techniques have facilitated the reconstruction of genome-scale metabolic networks for a wide variety of organisms from microbes to human cells. These models have been successfully used in multiple biotechnological applications. Despite these advancements, modeling cellular metabolism still presents many challenges. The aim of this Research Topic is not only to expose and consolidate the state-of-the-art in metabolic modeling approaches, but also to push this frontier beyond the current edge through the introduction of innovative solutions. The articles presented in this e-book address some of the main challenges in the field, including the integration of different modeling formalisms, the integration of heterogeneous data sources into metabolic models, explicit representation of other biological processes during phenotype simulation, and standardization efforts in the representation of metabolic models and simulation results.

Bacteria and Viruses

Bacteria and Viruses Book
Author : Britannica Educational Publishing
Publisher : Britannica Educational Publishing
Release : 2010-10-01
ISBN : 1615303766
Language : En, Es, Fr & De

GET BOOK

Book Description :

The sometimes insidious effects of bacterial diseases and viral infections can obscure the incredible significance of the microscopic organisms that cause them. Bacteria and viruses are among the oldest agents on Earth and reveal much about the planet’s past and evolution. Moreover, their utility in the development of new cures and treatments signals much about the future of biotechnology and medicine. This penetrating volume takes readers under the lens of a microscope to explore the structure, nature, and role of both bacteria and viruses as well as all other aspects of microbiology.

Systems Biology and Biotechnology of Escherichia coli

Systems Biology and Biotechnology of Escherichia coli Book
Author : Sang Yup Lee
Publisher : Springer Science & Business Media
Release : 2009-03-20
ISBN : 1402093942
Language : En, Es, Fr & De

GET BOOK

Book Description :

Systems biology is changing the way biological systems are studied by allowing us to examine the cell and organism as a whole. Systems biotechnology allows optimal design and development of upstream to downstream bioprocesses by taking a systems-approach. E. coli has been a model organism for almost all biological and biotechnological studies. This book brings together for the first time the state-of-the-art reviews by the world-leading experts on systems biology and biotechnological applications of E. coli. The topics covered include genomics and functional genomics, resources for systems biology, network analysis, genome-scale metabolic reconstruction, modelling and simulation, dynamic modelling and simulation, systems-level analysis of evolution, plasmids and expression systems, protein synthesis, production and export, engineering the central metabolism, synthetic biology, and systems metabolic engineering of E. coli. This book provides readers with guidance on how a complex biological system can be studied using E. coli as a model organism. It also presents how to perform synthetic biology and systems metabolic engineering studies on E. coli with successful examples, the approaches of which can be extended to other organisms. This book will be a complete resource for anyone interested in systems biology and biotechnology.

Metabolic Regulation

Metabolic Regulation Book
Author : Henry J. Vogel
Publisher : Academic Press
Release : 2014-05-10
ISBN : 1483265544
Language : En, Es, Fr & De

GET BOOK

Book Description :

Metabolic Pathways, Third Edition, Volume V: Metabolic Regulation presents the regulation of metabolism in terms of the control of protein synthesis. This book discusses the significance of the control of metabolism in regard to the remarkable mechanisms involved and to the provision of an effective conceptual framework for appreciating the workings of the cell. Organized into 15 chapters, this edition begins with an overview of the numerous and diversified activities of a living cell. This text then examines the ability of living organisms to regulate the storage and mobilization of metabolizable energy. Other chapters consider the cytochrome system that represents the final common path of oxidation and coupled phosphorylation in the eukaryotic cell. This book discusses as well the profound changes that cell metabolism undergoes during differentiation. The final chapter deals with the chemical and genetic bases of the induction response in Gram-positive bacteria. This book is a valuable resource for biologists and physicians.

Understanding Bacteria

Understanding Bacteria Book
Author : S. Srivastava
Publisher : Springer Science & Business Media
Release : 2013-03-14
ISBN : 9401701296
Language : En, Es, Fr & De

GET BOOK

Book Description :

The discipline of microbiology that deals with an amazingly diverse group of simple organisms, such as viruses, archaea, bacteria, algae, fungi, and protozoa, is an exciting field of Science. Starting as a purely descriptive field, it has transformed into a truly experimental and interdisciplinary science inspiring a number of investigators to generate th a wealth of information on the entire gamut of microbiology. The later part of 20 century has been a golden era with molecular information coming in to unravel interesting insights ofthe microbial world. Ever since they were brought to light through a pair of ground glasses by the Dutchman, Antony van Leeuwenhoek, in later half of 17th century, they have been studied most extensively throughout the next three centuries, and are still revealing new facets of life and its functions. The interest in them, therefore, continues even in the 21 st century. Though they are simple, they provide a wealth of information on cell biology, physiology, biochemistry, ecology, and genetics and biotechnology. They, thus, constitute a model system to study a whole variety of subjects. All this provided the necessary impetus to write several valuable books on the subject of microbiology. While teaching a course of Microbial Genetics for the last 35 years at Delhi University, we strongly felt the need for authentic compiled data that could give exhaustive background information on each of the member groups that constitute the microbial world.

Bioelectrosynthesis

Bioelectrosynthesis Book
Author : Aijie Wang,Wenzong Liu,Bo Zhang,Weiwei Cai
Publisher : John Wiley & Sons
Release : 2020-07-15
ISBN : 3527343814
Language : En, Es, Fr & De

GET BOOK

Book Description :

Introduces basic principles and mechanisms, covers new developments, and provides a different view of the main facets of bioelectrosynthesis Bioelectrosynthesis represents a promising approach for storing renewable energy or producing target chemicals in an energy-sustainable and low-cost way. This timely and important book systemically introduces the hot issues surrounding bioelectrosynthesis, including potential value-added products via bioelectrochemical system, reactor development of bioelectrosynthesis, and microbial biology on biofilm communities and metabolism pathways. It presents readers with unique viewpoints on basic principles and mechanisms along with new developments on reactor and microbial ecology. Beginning with a principle and products overview of bioelectrosynthesis, Bioelectrosynthesis: Principles and Technologies for Value-Added Products goes on to offer in-depth sections on: biogas production and upgrading technology via bioelectrolysis; organic synthesis on cathodes; chemical products and nitrogen recovery; external electron transfer and electrode material promotion; and the microbiology of bioelectrosynthesis. Topics covered include: hydrogen production from waste stream with microbial electrolysis cell; microbial electrolysis cell; inorganic compound synthesis in bioelectrochemical system; microbial growth, ecological, and metabolic characteristics in bioelectrosynthesis systems; microbial metabolism kinetics and interactions in bioelectrosynthesis system; and more. * Comprehensively covers all of the key issues of biolelectrosynthesis * Features contributions from top experts in the field * Examines the conversion of organic wastes to methane via electromethanogenesis; methane production at biocathodes; extracellular electron transport of electroactive biofilm; and more Bioelectrosynthesis: Principles and Technologies for Value-Added Products will appeal to chemists, electrochemists, environmental chemists, water chemists, microbiologists, biochemists, and graduate students involved in the field.

Systems Metabolic Engineering of Microbial Cell Factories for the Synthesis of Value added Chemicals

Systems Metabolic Engineering of Microbial Cell Factories for the Synthesis of Value added Chemicals Book
Author : Arul Mozhy Varman
Publisher : Unknown
Release : 2013
ISBN : 0987650XXX
Language : En, Es, Fr & De

GET BOOK

Book Description :

Microbial cell factories offer us an excellent opportunity for the conversion of many different cheaply available raw materials into valuable chemicals. Systems metabolic engineering aims at developing rational strategies for the engineering of microbial hosts by providing global level information of a cell. This dissertation focuses on metabolic engineering, bioprocess modeling and pathway analysis, to develop robust microbial cell factories for the synthesis of value-added chemicals. The following research tasks were completed in this regard. First, statistical models were developed for the prediction of product yields in engineered microbial cell factories - cerevisiae and Escherichia coli (Chapter 2). A large space of experimental data for chemical production from recent references was collected and a statistics-based model was developed to calculate production yield. The input variables (numerical or categorical variables) for the model represented the number of enzymatic steps in the biosynthetic pathway of interest, metabolic modifications, cultivation modes, nutrition and oxygen availability. In addition, the use of 13C-isotopomer analysis method was proposed for the accurate determination of product yields in engineered microbes under complex cultivation conditions (Chapter 3). Second, metabolic engineering of the cyanobacterium, Synechocystis sp. PCC 6803 was performed for synthesizing isobutanol under phototrophic conditions (Chapter 4). With the expression of the heterologous genes from the Ehrlich Pathway, by incorporating an in situ isobutanol harvesting system, and also by employing mixotrophic conditions, the engineered Synechocystis 6803 strain accumulated a maximum of ~300 mg/L of isobutanol in a 21 day culture. In addition, Synechocystis 6803 was engineered for the synthesis of D-lactic acid (Chapter 5), via overexpression of a novel D-lactate dehydrogenase (encoded by gldA101). The production of D-lactate was further improved by employing three strategies: (i) cofactor balancing, (ii) codon optimization, and (iii) process optimization. The engineered Synechocystis 6803 produced 2.2 g/L D-lactate under photoautotrophic conditions with acetate, the highest reported lactate titer among all known cyanobacterial strains. Finally, an E. coli cell factory was engineered to study the fermentation kinetics for scaled-up isobutanol production (Chapter 6). Through kinetic modeling (to describe the dynamics of biomass, products and glucose concentration) and isotopomer analysis, we have also offered metabolic insights into the performance trade-off between two engineered isobutanol producing E. coli strains (a high performance and a low performance strain). The kinetic model can also predict isobutanol production under different fermentation conditions. I and my colleagues have also demonstrated that E. coli cell factory can also be used for converting waste acetate into free fatty acids through metabolic engineering. In conclusion, the opportunities and commercial limitations with current biotechnology as well as the role of systems metabolic engineering for the development of high performance microbial cell factories were discussed (Chapter 7).

Regulation of Cell Metabolism

Regulation of Cell Metabolism Book
Author : G. E. W. Wolstenholme,Cecilia M. O'Connor
Publisher : John Wiley & Sons
Release : 2009-09-16
ISBN : 0470716630
Language : En, Es, Fr & De

GET BOOK

Book Description :

The Novartis Foundation Series is a popular collection of the proceedings from Novartis Foundation Symposia, in which groups of leading scientists from a range of topics across biology, chemistry and medicine assembled to present papers and discuss results. The Novartis Foundation, originally known as the Ciba Foundation, is well known to scientists and clinicians around the world.