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Analytical Techniques In Biosciences

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Analytical Techniques in Biosciences

Analytical Techniques in Biosciences Book
Author : Chukwuebuka Egbuna,Kingsley C. Patrick-Iwuanyanwu,Muhammad Ajmal Shah,Jonathan C. Ifemeje,Azhar Rasul
Publisher : Academic Press
Release : 2021-10-01
ISBN : 0128227990
Language : En, Es, Fr & De

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Book Description :

Analytical Techniques in Biosciences: From Basics to Applications presents comprehensive and up-to-date information on the various analytical techniques obtainable in bioscience research laboratories across the world. This book contains chapters that discuss the basic bioanalytical protocols and sample preparation guidelines. Commonly encountered analytical techniques, their working principles, and applications were presented. Techniques, considered in this book, include centrifugation techniques, electrophoretic techniques, chromatography, titrimetry, spectrometry, and hyphenated techniques. Subsequent chapters emphasize molecular weight determination and electroanalytical techniques, biosensors, and enzyme assay protocols. Other chapters detail microbial techniques, statistical methods, computational modeling, and immunology and immunochemistry. The book draws from experts from key institutions around the globe, who have simplified the chapters in a way that will be useful to early-stage researchers as well as advanced scientists. It is also carefully structured and integrated sequentially to aid flow, consistency, and continuity. This is a must-have reference for graduate students and researchers in the field of biosciences. • Presents basic analytical protocols and sample-preparation guidelines • Details the various analytical techniques, including centrifugation, spectrometry, chromatography, and titrimetry • Describes advanced techniques such as hyphenated techniques, electroanalytical techniques, and the application of biosensors in biomedical research • Presents biostatistical tools and methods and basic computational models in biosciences

Bioanalytics

Bioanalytics Book
Author : Friedrich Lottspeich,Joachim W. Engels
Publisher : John Wiley & Sons
Release : 2018-03-08
ISBN : 3527694463
Language : En, Es, Fr & De

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Book Description :

Analytical methods are the essential enabling tools of the modern biosciences. This book presents a comprehensive introduction into these analytical methods, including their physical and chemical backgrounds, as well as a discussion of the strengths and weakness of each method. It covers all major techniques for the determination and experimental analysis of biological macromolecules, including proteins, carbohydrates, lipids and nucleic acids. The presentation includes frequent cross-references in order to highlight the many connections between different techniques. The book provides a bird's eye view of the entire subject and enables the reader to select the most appropriate method for any given bioanalytical challenge. This makes the book a handy resource for students and researchers in setting up and evaluating experimental research. The depth of the analysis and the comprehensive nature of the coverage mean that there is also a great deal of new material, even for experienced experimentalists. The following techniques are covered in detail: - Purification and determination of proteins - Measuring enzymatic activity - Microcalorimetry - Immunoassays, affinity chromatography and other immunological methods - Cross-linking, cleavage, and chemical modification of proteins - Light microscopy, electron microscopy and atomic force microscopy - Chromatographic and electrophoretic techniques - Protein sequence and composition analysis - Mass spectrometry methods - Measuring protein-protein interactions - Biosensors - NMR and EPR of biomolecules - Electron microscopy and X-ray structure analysis - Carbohydrate and lipid analysis - Analysis of posttranslational modifications - Isolation and determination of nucleic acids - DNA hybridization techniques - Polymerase chain reaction techniques - Protein sequence and composition analysis - DNA sequence and epigenetic modification analysis - Analysis of protein-nucleic acid interactions - Analysis of sequence data - Proteomics, metabolomics, peptidomics and toponomics - Chemical biology

Fundamental Analytical Techniques for Biosciences

Fundamental Analytical Techniques for Biosciences Book
Author : Miriam Dwek
Publisher : Unknown
Release : 2015-05-08
ISBN : 9780470035023
Language : En, Es, Fr & De

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Book Description :

Download Fundamental Analytical Techniques for Biosciences book written by Miriam Dwek, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Analytical Biotechnology

Analytical Biotechnology Book
Author : Thomas G.M. Schalkhammer
Publisher : Birkhäuser
Release : 2012-11-28
ISBN : 3034881010
Language : En, Es, Fr & De

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Book Description :

Modern analytical biotechnology is focused on the use of a set of enabling platform technologies that provide contemporary, state-of-the-art tools for genomics, proteomics, metabolomics, drug discovery, screening, and analysis of natural product molecules. Thus, analytical biotechnology covers all areas of bioanalysis from biochips and nano-chemistry to biology and high throughput screening. Moreover, it aims to apply advanced automation and micro fabrica tion technology to the development of robotic and fluidic devices as well as integrated systems. This book focuses on enhancement technology development by promoting cross-disciplinary approaches directed toward solving key problems in biology and medicine. The scope thus brings under one umbrella many different techniques in allied areas. The purpose is to support and teach the fundamental principles and practical uses of major instrumental techniques. Major platforms are the use of immobilized molecules in biotechnology and bioanalysis, im munological techniques, immunological strip tests, fluorescence detection and confocal techniques, optical and electrochemical biosensors, biochips, micro dotting, novel transducers such as nano clusters, atomic force microscopy based techniques and analysis in complex media such as fermentation broth, plasma and serum. Techniques related to HPLC, capillary electrophoresis, gel electrophoresis, and mass spectrometry have not been included in this book but will be covered by further publications. Fundamentals in analytical biotechnology include basic and practical aspects of characterizing and analyzing DNA, proteins, and small metabolites.

Wilson and Walker s Principles and Techniques of Biochemistry and Molecular Biology

Wilson and Walker s Principles and Techniques of Biochemistry and Molecular Biology Book
Author : Andreas Hofmann,Samuel Clokie
Publisher : Cambridge University Press
Release : 2018-04-19
ISBN : 1108365256
Language : En, Es, Fr & De

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Book Description :

Bringing this best-selling textbook right up to date, the new edition uniquely integrates the theories and methods that drive the fields of biology, biotechnology and medicine, comprehensively covering both the techniques students will encounter in lab classes and those that underpin current key advances and discoveries. The contents have been updated to include both traditional and cutting-edge techniques most commonly used in current life science research. Emphasis is placed on understanding the theory behind the techniques, as well as analysis of the resulting data. New chapters cover proteomics, genomics, metabolomics, bioinformatics, as well as data analysis and visualisation. Using accessible language to describe concepts and methods, and with a wealth of new in-text worked examples to challenge students' understanding, this textbook provides an essential guide to the key techniques used in current bioscience research.

Instrumental Analysis in the Biological Sciences

Instrumental Analysis in the Biological Sciences Book
Author : M. H. Gordon
Publisher : Springer Science & Business Media
Release : 2012-12-06
ISBN : 1468415212
Language : En, Es, Fr & De

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Book Description :

Instrumental techniques of analysis have now moved from the confines of the chemistry laboratory to form an indispensable part of the analytical armoury of many workers involved in the biological sciences. It is now quite out of the question to considcr a laboratory dealing with the analysis of biological materials that is not equipped with an extensive range of instrumentation. Recent years have also seen a dramatic improvement in the ease with which such instruments can be used, and the quality and quantity of the analytical data that they can produce. This is due in no sm all part to the ubiquitous use of microprocessors and computers for instrumental control. However, under these circumstances there is areal danger of the analyst adopting a 'black box' mentality and not treating the analytical data produced in accordance with the limitations that may be inherent in the method used. Such a problem can only be overcome if the operator is fully aware of both the theoretical and instrumental constraints relevant to the technique in question. As the complexity and sheer volume of material in undergraduate courses increases, there is a tendency to reduce the amount of fundamental material that is taught prior to embarking on the more applied aspects. This is nowhere more apparent than in the teaching of instrumental techniques of analysis.

ANALYTICAL TECHNIQUES IN CHEMICAL AND BIOLOGICAL SCIENCES

ANALYTICAL TECHNIQUES IN CHEMICAL AND BIOLOGICAL SCIENCES Book
Author : Deep Mala
Publisher : Unknown
Release : 2018-04
ISBN : 9789386841599
Language : En, Es, Fr & De

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Book Description :

Download ANALYTICAL TECHNIQUES IN CHEMICAL AND BIOLOGICAL SCIENCES book written by Deep Mala, available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Statistical Methods in Biology

Statistical Methods in Biology Book
Author : S.J. Welham,S.A. Gezan,S.J. Clark,A. Mead
Publisher : CRC Press
Release : 2014-08-22
ISBN : 1439808783
Language : En, Es, Fr & De

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Book Description :

Written in simple language with relevant examples, Statistical Methods in Biology: Design and Analysis of Experiments and Regression is a practical and illustrative guide to the design of experiments and data analysis in the biological and agricultural sciences. The book presents statistical ideas in the context of biological and agricultural sciences to which they are being applied, drawing on relevant examples from the authors’ experience. Taking a practical and intuitive approach, the book only uses mathematical formulae to formalize the methods where necessary and appropriate. The text features extended discussions of examples that include real data sets arising from research. The authors analyze data in detail to illustrate the use of basic formulae for simple examples while using the GenStat® statistical package for more complex examples. Each chapter offers instructions on how to obtain the example analyses in GenStat and R. By the time you reach the end of the book (and online material) you will have gained: A clear appreciation of the importance of a statistical approach to the design of your experiments, A sound understanding of the statistical methods used to analyse data obtained from designed experiments and of the regression approaches used to construct simple models to describe the observed response as a function of explanatory variables, Sufficient knowledge of how to use one or more statistical packages to analyse data using the approaches described, and most importantly, An appreciation of how to interpret the results of these statistical analyses in the context of the biological or agricultural science within which you are working. The book concludes with a guide to practical design and data analysis. It gives you the understanding to better interact with consultant statisticians and to identify statistical approaches to add value to your scientific research.

Principles and Techniques of Biochemistry and Molecular Biology

Principles and Techniques of Biochemistry and Molecular Biology Book
Author : Keith Wilson,John Walker
Publisher : Cambridge University Press
Release : 2010-03-04
ISBN : 1107393493
Language : En, Es, Fr & De

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Book Description :

This best-selling undergraduate textbook provides an introduction to key experimental techniques from across the biosciences. It uniquely integrates the theories and practices that drive the fields of biology and medicine, comprehensively covering both the methods students will encounter in lab classes and those that underpin recent advances and discoveries. Its problem-solving approach continues with worked examples that set a challenge and then show students how the challenge is met. New to this edition are case studies, for example, that illustrate the relevance of the principles and techniques to the diagnosis and treatment of individual patients. Coverage is expanded to include a section on stem cells, chapters on immunochemical techniques and spectroscopy techniques, and additional chapters on drug discovery and development, and clinical biochemistry. Experimental design and the statistical analysis of data are emphasised throughout to ensure students are equipped to successfully plan their own experiments and examine the results obtained.

Advanced Chromatographic and Electromigration Methods in BioSciences

Advanced Chromatographic and Electromigration Methods in BioSciences Book
Author : I. Mikšík,F. Tagliaro,E. Tesarová,Z. Deyl
Publisher : Elsevier
Release : 1998-09-28
ISBN : 9780080858685
Language : En, Es, Fr & De

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Book Description :

This book deals with chromatographic and electrophoretic methods applied for the separation (quantitation and identification) of biologically relevant compounds. It is assumed that the potential reader is familiar with the basics of chromatographic and electromigration methods. Individual separation modes are dealt with to an extent which follows their applicability for biomedical purposes: liquid chromatography and electromigration methods are therefore highlighted. Each chapter is completed with a list of recent literature covering the 1987-1997 period, which can be used for further guidance of the reader in his/her own field. The chapters have been written by specialists in a particular area and with an emphasis on applications to the biomedical field. This implies that theoretical and instrumental aspects are kept to a minimum which allows the reader to understand the text. Considerable attention is paid to method selection, detection and derivatization procedures and troubleshooting. The majority of examples given represent the analyses of typical naturally-occurring mixtures. Adequate attention is paid to the role of the biological matrix and sample pretreatment, and special attention is given to forensic, toxicological and clinical applications. The book is completed with an extensive Index of Compounds Separated.

Techniques and Methods in Biology

Techniques and Methods in Biology Book
Author : Ghatak K. L.
Publisher : PHI Learning Pvt. Ltd.
Release : 2021-09-20
ISBN : 8120341716
Language : En, Es, Fr & De

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Book Description :

Download Techniques and Methods in Biology book written by Ghatak K. L., available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Ana Techniqs in Biotechnology

Ana Techniqs in Biotechnology Book
Author : Goutam Bhowmik
Publisher : Tata McGraw-Hill Education
Release : 2011
ISBN : 9780070700130
Language : En, Es, Fr & De

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Book Description :

'Analytical Techniques in Biotechnology' by Bhowmik and Bose offers a unique compendium of fundamental experiments, which forms the crucial foundation to understand this contemporary subject that has enormous impact on many other branches of life sciences. In addition to its simple and lucid language, the main focus of the book is to equip a beginner with the skill and ability required to conduct independent experimentation and research in laboratories. Carefully structured to cover the complete spectra of subjects under the umbrella of 'Biotechnology', this book is sure to prove a ready reference for students, faculties and researchers, all alike!

Advanced Mathematical Methods in Biosciences and Applications

Advanced Mathematical Methods in Biosciences and Applications Book
Author : Faina Berezovskaya,Bourama Toni
Publisher : Springer Nature
Release : 2019-09-19
ISBN : 3030157156
Language : En, Es, Fr & De

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Book Description :

Featuring contributions from experts in mathematical biology and biomedical research, this edited volume covers a diverse set of topics on mathematical methods and applications in the biosciences. Topics focus on advanced mathematical methods, with chapters on the mathematical analysis of the quasispecies model, Arnold’s weak resonance equation, bifurcation analysis, and the Tonnelier-Gerstner model. Special emphasis is placed on applications such as natural selection, population heterogeneity, polyvariant ontogeny in plants, cancer dynamics, and analytical solutions for traveling pulses and wave trains in neural models. A survey on quasiperiodic topology is also presented in this book. Carefully peer-reviewed, this volume is suitable for students interested in interdisciplinary research. Researchers in applied mathematics and the biosciences will find this book an important resource on the latest developments in the field. In keeping with the STEAM-H series, the editors hope to inspire interdisciplinary understanding and collaboration.

Research Techniques in Biological Sciences

Research Techniques in Biological Sciences Book
Author : G. S. Sandhu
Publisher : Unknown
Release : 1990
ISBN : 9788170413967
Language : En, Es, Fr & De

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Book Description :

This Is A Concise Laboratory Hand Book, Useful To All Those With An Interest In Histology, Histochemistry, Cytology, Endocrinology And Ecology. It Is One Of The Few Books That Give Practical Laboratory Methodology. The Methods Are Detailed Completely In A Step By Step Format That Allows The Laboratory Workers To Perform Any Procedure Reference To Secondary Source.There Are Numerous Techniques- Each Specially Detailing A Biochemical Or Microbiological Method Of Preparation Of Analysis To Enable The Researchers, Technicians, Student, Without The Need Of Library Search To Analyse A Biological Preparation. Not Only All Reagents Are Specified, But The Exact Methods Of Preparation Are Delineated So That No Further Calculations Are Necessary.

HPLC of Peptides and Proteins

HPLC of Peptides and Proteins Book
Author : Marie-Isabel Aguilar
Publisher : Humana
Release : 2003-12-15
ISBN : 9780896039773
Language : En, Es, Fr & De

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Book Description :

The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained. Today, HPLC in its various modes has become the pivotal technique in the characterization of peptides and proteins and currently plays a critical role in both our understanding of biological processes and in the development of peptide- and protein-based pharmaceuticals. The number of applications of HPLC in peptide and protein purification continues to expand at an extremely rapid rate. Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides and proteins that need to be highly purified. HPLC techniques are also used extensively in the isolation and characterization of novel proteins that will become increasingly important in the postgenomic age. The design of multidimensional purification schemes to achieve high levels of product purity further demonstrates the power of HPLC techniques not only in the characterization of cellular events, but also in the production of pepti- and protein-based therapeutics. HPLC continues to be at the heart of the analytical techniques with which scientists in both academia and in industry must arm themselves to be able to fully characterize the identity, purity, and potency of peptides and proteins.

Electrochemical Detection Techniques in the Applied Biosciences Fermentation and bioprocess control hygiene and environmental sciences

Electrochemical Detection Techniques in the Applied Biosciences  Fermentation and bioprocess control  hygiene and environmental sciences Book
Author : Anonim
Publisher : Unknown
Release : 1988
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

Download Electrochemical Detection Techniques in the Applied Biosciences Fermentation and bioprocess control hygiene and environmental sciences book written by , available in PDF, EPUB, and Kindle, or read full book online anywhere and anytime. Compatible with any devices.

Analytical Chemistry

Analytical Chemistry Book
Author : Séamus Higson,Séamus P J Higson
Publisher : Oxford University Press
Release : 2003-12-11
ISBN : 0987650XXX
Language : En, Es, Fr & De

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Book Description :

This clear and thorough introduction to modern analytical chemistry is essential for readers from all disciplines--including chemistry, forensic science, and the biosciences--where a familiarity with analytical techniques is required. Providing extensive coverage, it ranges from basic principles to the latest emerging techniques in the field. Numerous diagrams, worked examples, and self-assessment questions help readers test their understanding. (Midwest).

Laboratory Protocols in Applied Life Sciences

Laboratory Protocols in Applied Life Sciences Book
Author : Prakash Singh Bisen
Publisher : CRC Press
Release : 2014-02-26
ISBN : 1466553154
Language : En, Es, Fr & De

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Book Description :

As applied life science progresses, becoming fully integrated into the biological, chemical, and engineering sciences, there is a growing need for expanding life sciences research techniques. Anticipating the demands of various life science disciplines, Laboratory Protocols in Applied Life Sciences explores this development. This book covers a wide spectrum of areas in the interdisciplinary fields of life sciences, pharmacy, medical and paramedical sciences, and biotechnology. It examines the principles, concepts, and every aspect of applicable techniques in these areas. Covering elementary concepts to advanced research techniques, the text analyzes data through experimentation and explains the theory behind each exercise. It presents each experiment with an introduction to the topic, concise objectives, and a list of necessary materials and reagents, and introduces step-by-step, readily feasible laboratory protocols. Focusing on the chemical characteristics of enzymes, metabolic processes, product and raw materials, and on the basic mechanisms and analytical techniques involved in life science technological transformations, this text provides information on the biological characteristics of living cells of different origin and the development of new life forms by genetic engineering techniques. It also examines product development using biological systems, including pharmaceutical, food, and beverage industries. Laboratory Protocols in Applied Life Sciences presents a nonmathematical account of the underlying principles of a variety of experimental techniques in disciplines, including: Biotechnology Analytical biochemistry Clinical biochemistry Biophysics Molecular biology Genetic engineering Bioprocess technology Industrial processes Animal Plant Microbial biology Computational biology Biosensors Each chapter is self-contained and written in a style that helps students progress from basic to advanced techniques, and eventually design and execute their own experiments in a given field of biology.

Techniques in Molecular Systematics and Evolution

Techniques in Molecular Systematics and Evolution Book
Author : Rob DeSalle,Gonzalo Giribet,Ward Wheeler
Publisher : Birkhäuser
Release : 2013-12-01
ISBN : 3034881258
Language : En, Es, Fr & De

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Book Description :

The amount of information that can be obtained by using molecular techniques in evolution, systematics and ecology has increased exponentially over the last ten years. The need for more rapid and efficient methods of data acquisition and analysis is growing accordingly. This manual presents some of the most important techniques for data acquisition developed over the last years. The choice and justification of data analysis techniques is also an important and critical aspect of modern phylogenetic and evolutionary analysis and so a considerable part of this volume addresses this important subject. The book is mainly written for students and researchers from evolutionary biology in search for methods to acquire data, but also from molecular biology who might be looking for information on how data are analyzed in an evolutionary context. To aid the user, information on web-located sites is included wherever possible. Approaches that will push the amount of information which systematics will gather in the

Basic Bioscience Laboratory Techniques

Basic Bioscience Laboratory Techniques Book
Author : Philip L.R. Bonner,Alan J. Hargreaves
Publisher : John Wiley & Sons
Release : 2011-08-24
ISBN : 1119956447
Language : En, Es, Fr & De

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Book Description :

This unique, practical, pocket-sized guide and reference provides every first year bioscience student with all they need to know to prepare reagents correctly and perform fundamental laboratory techniques. It also helps them to analyse their data and present their findings, in addition to directing the reader, via a comprehensive list of references, to relevant further reading All of the core bioscience laboratory techniques are covered including: basic calculations and the preparation of solutions; aseptic techniques; microscopy techniques; cell fractionation ; spectrophotometry; chromatography of small and large molecules: electrophoresis of proteins and nucleic acids and data analysis. In addition the book includes clear, relevant diagrams and worked examples of calculations. In short, this is a 'must-have' for all first year bioscience students struggling to get to grips with this vitally important element of their course.