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Advanced Methods In Molecular Biology And Biotechnology

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Advanced Methods in Molecular Biology and Biotechnology

Advanced Methods in Molecular Biology and Biotechnology Book
Author : Khalid Z. Masoodi,Sameena Maqbool Lone,Rovidha Saba Rasool
Publisher : Academic Press
Release : 2020-11-10
ISBN : 012824450X
Language : En, Es, Fr & De

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Book Description :

Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology Features clear, step-by-step instruction for applying the techniques covered Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment

Molecular Biology and Biotechnology

Molecular Biology and Biotechnology Book
Author : John M. Walker,Ralph Rapley,E. B. Gingold
Publisher : Royal Society of Chemistry
Release : 2000
ISBN : 9780854046065
Language : En, Es, Fr & De

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Book Description :

This volume provides a revised and updated introduction to the techniques of molecular biology and its industrial applications. It should be of particular benefit to undergraduates and researchers in other biological areas.

Molecular Biology and Biotechnology

Molecular Biology and Biotechnology Book
Author : Robert A. Meyers
Publisher : John Wiley & Sons
Release : 1995-06-29
ISBN : 9780471186342
Language : En, Es, Fr & De

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Book Description :

Essential for the top of every desk and laboratory bench, this single volume reference is the first and last resource required to answer any question relating to molecular biology, biotechnology, or molecular medicine. This work contains the theory and techniques for understanding, modifying, manipulating, expressing, and synthesizing biological molecules, conformations, and aggregates. Over 250 carefully reviewed articles written by 378 eminent scientists in the field, provide detailed and readable coverage of today's most relevant topics including life processes at the molecular level, genetic disease diagnosis and genetic therapy, the theory and techniques for understanding, manipulating, and synthesizing biological molecules and their aggregates.

Molecular Biology Techniques

Molecular Biology Techniques Book
Author : Sue Carson,Heather Miller,Melissa C. Srougi,D. Scott Witherow
Publisher : Academic Press
Release : 2019-03-05
ISBN : 0128157755
Language : En, Es, Fr & De

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Book Description :

Molecular Biology Techniques: A Classroom Laboratory Manual, Fourth Edition is a must-have collection of methods and procedures on how to create a single, continuous, comprehensive project that teaches students basic molecular techniques. It is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology—or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students will gain hands-on experience on subcloning a gene into an expression vector straight through to the purification of the recombinant protein. Presents student-tested labs proven successful in real classroom laboratories Includes a test bank on a companion website for additional testing and practice Provides exercises that simulate a cloning project that would be performed in a real research lab Includes a prep-list appendix that contains necessary recipes and catalog numbers, providing staff with detailed instructions

Computational Methods in Molecular Biology

Computational Methods in Molecular Biology Book
Author : S.L. Salzberg,D.B. Searls,S. Kasif
Publisher : Elsevier
Release : 1998-06-19
ISBN : 9780080860930
Language : En, Es, Fr & De

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Book Description :

Computational biology is a rapidly expanding field, and the number and variety of computational methods used for DNA and protein sequence analysis is growing every day. These algorithms are extremely valuable to biotechnology companies and to researchers and teachers in universities. This book explains the latest computer technology for analyzing DNA, RNA, and protein sequences. Clear and easy to follow, designed specifically for the non-computer scientist, it will help biologists make better choices on which algorithm to use. New techniques and demonstrations are elucidated, as are state-of-the-art problems, and more advanced material on the latest algorithms. The primary audience for this volume are molecular biologists working either in biotechnology companies or academic research environments, individual researchers and the institutions they work for, and students. Any biologist who relies on computers should want this book. A secondary audience will be computer scientists developing techniques with applications in biology. An excellent reference for leading techniques, it will also help introduce computer scientists to the biology problems. This is an outstanding work which will be ideal for the increasing number of scientists moving into computational biology.

Molecular Biology Techniques

Molecular Biology Techniques Book
Author : Walt Ream,Katharine G. Field
Publisher : Academic Press
Release : 1998-11-17
ISBN : 0080536824
Language : En, Es, Fr & De

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Book Description :

This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. Key Features * Provides well-tested experimental protocols for each technique * Lists the reagents and preparation of each experiment separately * Contains a complete schedule of experiments and the preparation required * Includes study questions at the end of each chapter

Advanced Biotechnology

Advanced Biotechnology Book
Author : R C Dubey
Publisher : S. Chand Publishing
Release : 2014
ISBN : 812194290X
Language : En, Es, Fr & De

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Book Description :

The book embodies 22 chapters covering various important disciplines of biotechnology, such as cell biology, molecular biology, molecular genetics, biophysical methods, genomics and proteomics, metagenomics, enzyme technology, immune-technology, transgenic plants and animals, industrial microbiology and environmental biotechnology. The book is illustrative. It is written in a simple language

Advanced Molecular Genetics

Advanced Molecular Genetics Book
Author : Alfred Pühler,Kenneth N. Timmis
Publisher : Springer Science & Business Media
Release : 2012-12-06
ISBN : 3642693059
Language : En, Es, Fr & De

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Book Description :

The development of powerful new techniques and refmements of tech niques in molecular genetics in recent years, and the surge in interest in biotechnology based on genetic methods, have heralded a new golden age in molecular genetics, and stimulated in diverse disciplines much interest in the technologies themselves and their potential uses in basic and applied biomedical sciences. Although some excellent specialist laboratory manuals (especially the Cold Spring Harbor Laboratory manuals by I. H. Miller; R. W. Davies et al. ; and T. Maniatis et al. ) on certain chapters of molecular genetics exist, no general text that covers a broad spectrum of the sub ject has thus far been published. The purpose of this manual is to pre sent most, though of necessity not all of the important methods of molecular genetics, in a series of simple experiments, many of which can be readily accomplished by the microbiologist, biochemist or biotechnologist that has had only limited exposure to genetics. The remainder of the experiments require either greater familiarity with the subject, or guidance by someone with such experience. The book should, therefore, not only enable individuals to acquire new proce dures for ongoing projects, but also serve as a basis for the teaching of molecular genetic techniques in formal predoctoral and postdoctoral laboratory courses.

Methods in Plant Molecular Biology and Biotechnology

Methods in Plant Molecular Biology and Biotechnology Book
Author : Bernard R. Glick
Publisher : CRC Press
Release : 2018-05-04
ISBN : 1351091395
Language : En, Es, Fr & De

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Book Description :

Methods in Plant Molecular Biology and Biotechnology emphasizes a variety of well-tested methods in plant molecular biology and biotechnology. For each detailed and tested protocol presented, a brief overview of the methodology is provided. This overview considers why the protocol is used, what other comparable methods are available, and what limitations can be expected with the protocol. Other chapters in the book present overviews regarding how to approach particular problems and introduce unique methods - such as how to use computer methodology to study isolated genes. The book will be a practical reference for plant physiologists, plant molecular biologists, phytopathologists, and microbiologists.

Molecular Biology Techniques

Molecular Biology Techniques Book
Author : Sue Carson,Susan Carson,Heather Miller,D. Scott Witherow
Publisher : Academic Press
Release : 2012
ISBN : 0123855446
Language : En, Es, Fr & De

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Book Description :

This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project" approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions

Calculations for Molecular Biology and Biotechnology

Calculations for Molecular Biology and Biotechnology Book
Author : Frank H. Stephenson
Publisher : Academic Press
Release : 2010-07-30
ISBN : 9780123756916
Language : En, Es, Fr & De

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Book Description :

Calculations for Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory, Second Edition, provides an introduction to the myriad of laboratory calculations used in molecular biology and biotechnology. The book begins by discussing the use of scientific notation and metric prefixes, which require the use of exponents and an understanding of significant digits. It explains the mathematics involved in making solutions; the characteristics of cell growth; the multiplicity of infection; and the quantification of nucleic acids. It includes chapters that deal with the mathematics involved in the use of radioisotopes in nucleic acid research; the synthesis of oligonucleotides; the polymerase chain reaction (PCR) method; and the development of recombinant DNA technology. Protein quantification and the assessment of protein activity are also discussed, along with the centrifugation method and applications of PCR in forensics and paternity testing. Topics range from basic scientific notations to complex subjects like nucleic acid chemistry and recombinant DNA technology Each chapter includes a brief explanation of the concept and covers necessary definitions, theory and rationale for each type of calculation Recent applications of the procedures and computations in clinical, academic, industrial and basic research laboratories are cited throughout the text New to this Edition: Updated and increased coverage of real time PCR and the mathematics used to measure gene expression More sample problems in every chapter for readers to practice concepts

Analytical Techniques in Biochemistry and Molecular Biology

Analytical Techniques in Biochemistry and Molecular Biology Book
Author : Rajan Katoch
Publisher : Springer Science & Business Media
Release : 2011-07-19
ISBN : 9781441997852
Language : En, Es, Fr & De

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Book Description :

Advances in biochemistry now allow us to control living systems in ways that were undreamt of a decade ago. This volume guides researchers and students through the full spectrum of experimental protocols used in biochemistry, plant biology and biotechnology.

An Introduction to Molecular Biotechnology

An Introduction to Molecular Biotechnology Book
Author : Michael Wink,Renate FitzRoy
Publisher : John Wiley & Sons
Release : 2006-10-02
ISBN : 9783527314126
Language : En, Es, Fr & De

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Book Description :

On 800 pages this textbook provides students and professionals in life sciences, pharmacy and biochemistry with a very detailed introduction to molecular and cell biology, including standard techniques, key topics, and biotechnology in industry.

Molecular Techniques in Food Biology

Molecular Techniques in Food Biology Book
Author : Aly Farag El Sheikha,Robert E. Levin,Jianping Xu
Publisher : John Wiley & Sons
Release : 2018-04-09
ISBN : 111937460X
Language : En, Es, Fr & De

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Book Description :

Molecular Techniques in Food Biology: Safety, Biotechnology, Authenticity and Traceability explores all aspects of microbe-food interactions, especially as they pertain to food safety. Traditional morphological, physiological, and biochemical techniques for the detection, differentiation, and identification of microorganisms have severe limitations. As an alternative, many of those responsible for monitoring food safety are turning to molecular tools for identifying foodborne microorganisms. This book reviews the latest molecular techniques for detecting, identifying, and tracing microorganisms in food, addressing both good foodborne microbes, such as those used for fermentation and in probiotics, and harmful ones responsible for foodborne illness and food quality control problems. Molecular Techniques in Food Biology: Safety, Biotechnology, Authenticity and Traceability brings together contributions by leading international authorities in food biology from academe, industry, and government. Chapters cover food microbiology, food mycology, biochemistry, microbial ecology, food biotechnology and bio-processing, food authenticity, food origin traceability, and food science and technology. Throughout, special emphasis is placed on novel molecular techniques relevant to food biology research and for monitoring and assessing food safety and quality. Brings together contributions from scientists at the leading edge of the revolution in molecular food biology Explores how molecular techniques can satisfy the dire need to deepen our understanding of how microbial communities develop in foods of all types and in all forms Covers all aspects of food safety and hygiene, microbial ecology, food biotechnology and bio-processing, food authenticity, food origin traceability, and more Fills a yawning gap in the world literature on food traceability using molecular techniques This book is an important working resource for professionals in the agricultural, food and biomedical sciences, as well as government personnel involved in food regulation and safety. It is also an excellent reference for advanced students in agriculture, food science and food technology, biochemistry, microbiology, and biotechnology, as well as academic researchers in those fields.

Trends in Insect Molecular Biology and Biotechnology

Trends in Insect Molecular Biology and Biotechnology Book
Author : Dhiraj Kumar,Chengliang Gong
Publisher : Springer
Release : 2018-04-03
ISBN : 331961343X
Language : En, Es, Fr & De

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Book Description :

This book provides an overview on the basics in insect molecular biology and presents the most recent developments in several fields such as insect genomics and proteomics, insect pathology and applications of insect derived compounds in modern research. The book aims to provide a common platform for the molecular entomologist to stimulate further research in insect molecular biology and biotechnology. Insects are one of the most versatile groups of the animal kingdom. Due to their large population sizes and adaptability since long they attract researchers’ interest as efficient resource for agricultural and biotechnological purposes. Several economically important insects such as Silkworms, Honey Bee, Lac and Drosophila or Termites were established as invertebrate model organisms. Starting with the era of genetic engineering, a broad range of molecular and genetic tools have been developed to study the molecular biology of these insects in detail and thus opened up a new horizon for multidisciplinary research. Nowadays, insect derived products are widely used in biomedical and biotechnology industries. The book targets researchers from both academia and industry, professors and graduate students working in molecular biology, biotechnology and entomology.

Advanced Methods in Protein Microsequence Analysis

Advanced Methods in Protein Microsequence Analysis Book
Author : Brigitte Wittmann-Liebold,Johann Salnikow
Publisher : Springer Science & Business Media
Release : 2012-12-06
ISBN : 3642715346
Language : En, Es, Fr & De

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Book Description :

Much of the recent spectacular progress in the biological sciences can be at tributed ot the ability to isolate, analyze, and structurally characterize proteins and peptides which are present in cells and cellular organelles in only very small amounts. Recent advances in protein chemistry and in particular the application of new micromethods have led to fruitful advances in the understanding of basic cellular processes. Areas where protein-chemical studies have resulted in interest ing discoveries include the peptide hormones and their release factors, growth factors and oncogenes, bioenergetics, proton pumps and ion pumps and chan nels, topogenesis and protein secretion, molecular virology and immunology, membrane protein analysis, and receptor research. In fact, the key methods are now on hand to unravel many of the major outstanding problems of molecular biology and in particular questions of fundamental interest which relate to devel opmental biology and specificity in cell-cell interaction. In this volume we have assembled descriptions of procedures which have re cently been shown to be efficaceous for the isolation, purification, and chemical characterization of proteins and peptides that are only available in minute amounts. Emphasis is placed on well-established micromethods which have been tested and found useful in many laboratories by experienced investigators. The chapters are written by specialists, and describe a range of sensitive techniques which can be used by researchers working in laboratories with only modest resources and equipment.

Principles and Techniques of Biochemistry and Molecular Biology

Principles and Techniques of Biochemistry and Molecular Biology Book
Author : Keith Wilson,John Walker
Publisher : Cambridge University Press
Release : 2010-03-04
ISBN : 0521516358
Language : En, Es, Fr & De

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Book Description :

Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.

Biotechnology 3 Including Molecular Biology Biophysics

Biotechnology 3  Including Molecular Biology Biophysics Book
Author : S. Mahesh
Publisher : New Age International
Release : 2007
ISBN : 9788122414998
Language : En, Es, Fr & De

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Book Description :

The Present Book Covers The Syllabus Of Biotechnology-3 Prescribed By Bangalore University And Second Year Degree, Biotechnology Vocational Course (Ugc), New Delhi. The Book Endeavours To Furnish A Simple, Understandable Text For Students. This Book Has Been Divided Into Two Major Parts, Part A Includes Molecular Biology And Part B Includes Biophysics.One Of The Highlights Of This Book Is That, Part B (Biophysics) Elaborates The Information On Biological Science At The Backdrop Of Physics Concepts.